p2y12  (Alomone Labs)


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    Alomone Labs p2y12
    P2y12, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    p2y12 - by Bioz Stars, 2022-08
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    Alomone Labs anti p2y12 receptor extracellular antibody
    Generation and characterization of iMg (a) Timeline for iMg differentiation and HIV infection. (b) Immunostaining for microglia specific markers: CX3CR1, TMEM119, IBA1, and <t>P2RY12.</t> All cultures were stained for DAPI. (c) Bulk RNAseq normalized read counts of specific microglia markers from iMg and MDMs. n=4 iMg, n=3 MDMs. Benjamini-Hochberg, FDR 0.01, **p
    Anti P2y12 Receptor Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p2y12 receptor extracellular antibody/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p2y12 receptor extracellular antibody - by Bioz Stars, 2022-08
    91/100 stars
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    85
    Alomone Labs anti p2y receptor antibodies
    Expression of <t>P2Y</t> receptors in osteoblasts. Using flow cytometry analysis, P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 11 , P2Y 12 , and P2Y 13 receptors were detected in osteoblasts using their respective Alomone Labs antibodies ( open histograms ). Incubating the antibody with the control peptide antigen was used as a negative control ( filled histograms ). Adapted from reference 41 with permission of Elsevier
    Anti P2y Receptor Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p2y receptor antibodies/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p2y receptor antibodies - by Bioz Stars, 2022-08
    85/100 stars
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    Generation and characterization of iMg (a) Timeline for iMg differentiation and HIV infection. (b) Immunostaining for microglia specific markers: CX3CR1, TMEM119, IBA1, and P2RY12. All cultures were stained for DAPI. (c) Bulk RNAseq normalized read counts of specific microglia markers from iMg and MDMs. n=4 iMg, n=3 MDMs. Benjamini-Hochberg, FDR 0.01, **p

    Journal: bioRxiv

    Article Title: Neuroinflammation and EIF2 signaling persist in an HiPSC tri-culture model of HIV infection despite antiretroviral treatment

    doi: 10.1101/779025

    Figure Lengend Snippet: Generation and characterization of iMg (a) Timeline for iMg differentiation and HIV infection. (b) Immunostaining for microglia specific markers: CX3CR1, TMEM119, IBA1, and P2RY12. All cultures were stained for DAPI. (c) Bulk RNAseq normalized read counts of specific microglia markers from iMg and MDMs. n=4 iMg, n=3 MDMs. Benjamini-Hochberg, FDR 0.01, **p

    Article Snippet: Antibodies used for immunofluorescence: Chicken anti-MAP2 (Abcam ab5392, RRID:AB_2138153, 1:500); Mouse anti-PSD-95 clone K28/43 (NeuroMab 75-028, RRID:AB_2292909, 1:500); Mouse anti-Synaptophysin clone SY38 (Millipore MAB5258-20UG, RRID:AB_95185, 1:250); Mouse anti-Nestin clone 10C2 (Millipore MAB5326, RRID:AB_11211837, 1:200); Rabbit anti-Thrombospondin-1 (Abcam ab85762, RRID:AB_10674322, 1:250); Mouse anti-Glutamine Synthetase (Millipore MAB302, RRID:AB_2110656, 1:500); Rabbit anti-SOX9 (Abcam ab185230, RRID:AB_2715497, 1:250); Rabbit anti-CX3CR1 (Abcam ab8021, RRID:AB_306203, 1:500); Rabbit anti-TMEM119 (Abcam ab185333, RRID:AB_2687894, 1:200); Rabbit anti-IBA1 (Wako 019-19741, RRID:AB_839504, 1:500); Rabbit anti-P2RY12 (Alomone Labs APR-020, RRID:AB_11121048, 1:100); Rat anti-LAMP1 (Abcam ab25245, RRID:AB_449893, 1:500); Mouse anti-HIV1 p24 [39/5.4A] (Abcam ab9071, RRID:AB_306981, 1:500); Rabbit anti-Human Nanog (Cell Signaling Technology 3580, RRID:AB_2150399, 1:800); Mouse anti-NCAM Clone 2-2b (Millipore MAB5324, RRID:AB_95211, 1:250); Rabbit anti-OCT-4 (Cell Signaling Technology 2750, RRID:AB_823583, 1:200); Rabbit anti-SOX2 (Millipore AB5603, RRID:AB_2286686, 1:100); Rabbit anti-CCR5 (Thermo Fisher Scientific pa5-29011, RRID:AB_2546487, 1:500); Mouse anti-GLAST (Miltenyi Biotec 130-095-822, RRID:AB_10829302, 1:50); Mouse anti-GFAP (Sigma-Aldrich SAB1405864, RRID:AB_10739114, 1:10,000); Mouse anti-Beta-Actin (Cell Signaling Technology 3700, RRID:AB_2242334, 1:10,000); Rabbit anti-EAAC1 (Santa Cruz Biotechnology sc-25658, RRID:AB_2190727, 1:50); Rabbit GLT-1 (Jeff Rothstein Lab 1:5,000); Goat anti-mouse IgG (H+L) Alexa Fluor 488 (Thermo Fisher Scientific A-11029, RRID:AB_138404, 1:500); Goat anti-rabbit IgG (H+L) Alexa Fluor 488 (Thermo Fisher Scientific A-11034, RRID:AB_2576217, 1:500); Goat anti-mouse IgG (H+L) Alexa Fluor 568 (Thermo Fisher Scientific A-11004, RRID:AB_2534072, 1:500); Goat anti-rabbit IgG (H+L) Alexa Fluor 568 (Thermo Fisher Scientific A-11036, RRID:AB_10563566, 1:500); Goat anti-rat IgG (H+L) Alexa Fluor 568 (Molecular Probes A-11077, RRID:AB_141874, 1:500); Goat anti-rat IgG (H+L) Alexa Fluor 680 (Molecular Probes A-21096, RRID:AB_141554, 1:500); Goat anti-Chicken IgY (H+L) DyLight 680 (Thermo Fisher Scientific SA5-10074, RRID:AB_2556654, 1:500).

    Techniques: Infection, Immunostaining, Staining

    Expression of P2Y receptors in osteoblasts. Using flow cytometry analysis, P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 11 , P2Y 12 , and P2Y 13 receptors were detected in osteoblasts using their respective Alomone Labs antibodies ( open histograms ). Incubating the antibody with the control peptide antigen was used as a negative control ( filled histograms ). Adapted from reference 41 with permission of Elsevier

    Journal: Purinergic Signalling

    Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research

    doi: 10.1007/s11302-011-9278-z

    Figure Lengend Snippet: Expression of P2Y receptors in osteoblasts. Using flow cytometry analysis, P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 11 , P2Y 12 , and P2Y 13 receptors were detected in osteoblasts using their respective Alomone Labs antibodies ( open histograms ). Incubating the antibody with the control peptide antigen was used as a negative control ( filled histograms ). Adapted from reference 41 with permission of Elsevier

    Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].

    Techniques: Expressing, Flow Cytometry, Cytometry, Negative Control

    Expression of P2Y receptors in Müller cells of the adult rat and mouse retinal slices. Immunohistochemical staining was accomplished using anti-P2Y 2 receptor (#APR-010) and anti-P2Y 6 receptor (#APR-011) antibodies. a No distinct immunoreactivity for the P2Y 2 receptor ( red ) was observed in Müller cells. However, ganglion cells ( asterisk ) and structures within the inner plexiform layer ( IPL ), inner nuclear layer ( INL ), and outer plexiform layer ( OPL ) display faint staining. b P2Y 4 receptor ( red ) is highly expressed in Müller cell end-feet ( arrows ), as detected using anti-P2Y 4 receptor antibody (#APR-006). Additionally, a band of globular structures, probably representing synapse of bipolar cells in the ON-sublamina ( ON ) of the IPL, is stained for this receptor. c The staining pattern of the P2Y 6 receptor ( red ) is similar to that of P2Y 2 . The insets in ( b ) and ( c ) show the ganglion cell layer ( GCL ) and the INL, respectively, at higher magnification. ONL outer nuclear layer, OFF OFF-sublamina of the IPL, PRS photoreceptor segments. Scale bars , 20 μm; inset scale bars , 10 μm. d Immunohistochemical staining was carried out using anti-P2Y 4 receptor antibody. The slices were co-stained against the glial cell marker cellular retinaldehyde-binding protein ( CRALBP ); co-labeling yielded a yellow-orange merge signal. Arrows , Müller cell end-feet; arrowheads , Müller cell somata. GCL ganglion cell layer, IPL inner plexiform layer, NFL nerve fiber layer, ONL outer nuclear layer, PRS , photoreceptor segments. Scale bars , 20 μm. a–c Adapted from reference 12 with permission of John Wiley and Sons. d Adapted from reference 13 with permission of Wiley-Blackwell

    Journal: Purinergic Signalling

    Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research

    doi: 10.1007/s11302-011-9278-z

    Figure Lengend Snippet: Expression of P2Y receptors in Müller cells of the adult rat and mouse retinal slices. Immunohistochemical staining was accomplished using anti-P2Y 2 receptor (#APR-010) and anti-P2Y 6 receptor (#APR-011) antibodies. a No distinct immunoreactivity for the P2Y 2 receptor ( red ) was observed in Müller cells. However, ganglion cells ( asterisk ) and structures within the inner plexiform layer ( IPL ), inner nuclear layer ( INL ), and outer plexiform layer ( OPL ) display faint staining. b P2Y 4 receptor ( red ) is highly expressed in Müller cell end-feet ( arrows ), as detected using anti-P2Y 4 receptor antibody (#APR-006). Additionally, a band of globular structures, probably representing synapse of bipolar cells in the ON-sublamina ( ON ) of the IPL, is stained for this receptor. c The staining pattern of the P2Y 6 receptor ( red ) is similar to that of P2Y 2 . The insets in ( b ) and ( c ) show the ganglion cell layer ( GCL ) and the INL, respectively, at higher magnification. ONL outer nuclear layer, OFF OFF-sublamina of the IPL, PRS photoreceptor segments. Scale bars , 20 μm; inset scale bars , 10 μm. d Immunohistochemical staining was carried out using anti-P2Y 4 receptor antibody. The slices were co-stained against the glial cell marker cellular retinaldehyde-binding protein ( CRALBP ); co-labeling yielded a yellow-orange merge signal. Arrows , Müller cell end-feet; arrowheads , Müller cell somata. GCL ganglion cell layer, IPL inner plexiform layer, NFL nerve fiber layer, ONL outer nuclear layer, PRS , photoreceptor segments. Scale bars , 20 μm. a–c Adapted from reference 12 with permission of John Wiley and Sons. d Adapted from reference 13 with permission of Wiley-Blackwell

    Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].

    Techniques: Expressing, Immunohistochemistry, Staining, Marker, Binding Assay, Labeling

    Differential expression of P2Y receptors in glial following retinal detachment. Immunohistochemical staining of control retinas and from detached areas of porcine retinas 7 days after surgery was done using anti-P2Y 1 receptor (#APR-009), anti-P2Y 2 receptor (#APR-010), and anti-P2Y 4 receptor (#APR-006) antibodies ( red ). Immunoreactivities for P2Y 1 , P2Y 2 , and P2Y 4 receptor proteins ( red ) coincide with that of intermediate filament constituents vimentin ( green and yellow for merging signal). Arrows , vimentin-positive fibers in the outer retina that were also stained for P2Y 2 protein. GCL ganglion cell layer, INL inner nuclear layer, IPL inner plexiform layer, NFL nerve fiber layer, ONL outer nuclear layer. Scale bars , 20 μm. Adapted from reference 15 with permission of Association for Research in Vision and Ophthalmology

    Journal: Purinergic Signalling

    Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research

    doi: 10.1007/s11302-011-9278-z

    Figure Lengend Snippet: Differential expression of P2Y receptors in glial following retinal detachment. Immunohistochemical staining of control retinas and from detached areas of porcine retinas 7 days after surgery was done using anti-P2Y 1 receptor (#APR-009), anti-P2Y 2 receptor (#APR-010), and anti-P2Y 4 receptor (#APR-006) antibodies ( red ). Immunoreactivities for P2Y 1 , P2Y 2 , and P2Y 4 receptor proteins ( red ) coincide with that of intermediate filament constituents vimentin ( green and yellow for merging signal). Arrows , vimentin-positive fibers in the outer retina that were also stained for P2Y 2 protein. GCL ganglion cell layer, INL inner nuclear layer, IPL inner plexiform layer, NFL nerve fiber layer, ONL outer nuclear layer. Scale bars , 20 μm. Adapted from reference 15 with permission of Association for Research in Vision and Ophthalmology

    Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].

    Techniques: Expressing, Immunohistochemistry, Staining

    Expression of P2Y receptors in rat duodenal epithelium. Immunohistochemical staining was done using anti-P2Y 1 receptor antibody (#APR-009), anti-P2Y 2 receptor antibody (#APR-010), and anti-P2Y 6 receptor antibody (#APR-011). a P2Y 1 is expressed along the brush border membranes of villous cells. P2Y 2 and P2Y 4 receptors are not expressed ( b , c ). d P2Y 6 receptor is diffusely expressed in the villous cells and interstitium. Adapted from reference 31 with permission of John Wiley and Sons

    Journal: Purinergic Signalling

    Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research

    doi: 10.1007/s11302-011-9278-z

    Figure Lengend Snippet: Expression of P2Y receptors in rat duodenal epithelium. Immunohistochemical staining was done using anti-P2Y 1 receptor antibody (#APR-009), anti-P2Y 2 receptor antibody (#APR-010), and anti-P2Y 6 receptor antibody (#APR-011). a P2Y 1 is expressed along the brush border membranes of villous cells. P2Y 2 and P2Y 4 receptors are not expressed ( b , c ). d P2Y 6 receptor is diffusely expressed in the villous cells and interstitium. Adapted from reference 31 with permission of John Wiley and Sons

    Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].

    Techniques: Expressing, Immunohistochemistry, Staining

    Expression of P2Y receptors in rat distal colon. All P2Y immunohistochemical staining was done using Alomone Labs anti-P2Y receptor antibodies. a Double immunolabeling reveals that P2Y 1 is expressed in enteric smooth muscle cells and S100 in enteric glial cells. b In the myenteric plexus, P2Y 2 is detected in neurons. c P2Y 4 is detected in enteric glial cells. d P2Y 6 is expressed in vascular and enteric smooth muscle cells. e P2Y 11 is detected in enteric glial cells and P2Y 12 is expressed in enteric nerves ( f ). Adapted from reference 30 with permission of Elsevier

    Journal: Purinergic Signalling

    Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research

    doi: 10.1007/s11302-011-9278-z

    Figure Lengend Snippet: Expression of P2Y receptors in rat distal colon. All P2Y immunohistochemical staining was done using Alomone Labs anti-P2Y receptor antibodies. a Double immunolabeling reveals that P2Y 1 is expressed in enteric smooth muscle cells and S100 in enteric glial cells. b In the myenteric plexus, P2Y 2 is detected in neurons. c P2Y 4 is detected in enteric glial cells. d P2Y 6 is expressed in vascular and enteric smooth muscle cells. e P2Y 11 is detected in enteric glial cells and P2Y 12 is expressed in enteric nerves ( f ). Adapted from reference 30 with permission of Elsevier

    Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].

    Techniques: Expressing, Immunohistochemistry, Staining, Immunolabeling

    Confocal images of examples of double immunofluorescence labeling to characterize the colocalization of P2X 1,2,4,7 and P2Y 1,6,12 receptor subtypes on a–f OX42- or g–o GSA-B4-labeled microglial cells in the NAc of rats 4 days after stab-wound injury ( thin arrow process-bearing microglial cell; thick arrow activated microglial cell) ( scale bars : a, b = 20 μm; c, d = 100 μm; e–i = 20 μm; j, k = 100 μm; l, m = 20 μm; n, o = 50 μm)

    Journal: Purinergic Signalling

    Article Title: Involvement of P2X and P2Y receptors in microglial activation in vivo

    doi: 10.1007/s11302-007-9082-y

    Figure Lengend Snippet: Confocal images of examples of double immunofluorescence labeling to characterize the colocalization of P2X 1,2,4,7 and P2Y 1,6,12 receptor subtypes on a–f OX42- or g–o GSA-B4-labeled microglial cells in the NAc of rats 4 days after stab-wound injury ( thin arrow process-bearing microglial cell; thick arrow activated microglial cell) ( scale bars : a, b = 20 μm; c, d = 100 μm; e–i = 20 μm; j, k = 100 μm; l, m = 20 μm; n, o = 50 μm)

    Article Snippet: Double-immunofluorescence studies After washing with TBS (0.05 M; pH 7.6) and blocking with normal goat serum (1% NGS) in TBS, slices were incubated in an antibody mixture of mouse anti-OX 42 (CD11b; 1:100) and rabbit anti-P2X receptor antibodies (P2X1 1:400; P2X2 1:400; P2X4 1:400; P2X5 1:400; P2X6 1:400; P2X7 1:1,000) or P2Y receptor antibodies [P2Y1 1:400; P2Y2 1:1,000; P2Y4 1:1,000; P2Y12 1:400 (Alomone Labs); P2Y1 : 1:1,500 (GlaxoSmithKline); P2Y6 1:200 (Santa Cruz Biotechnology, Inc.)] with 0.1% Triton X-100 in 1% NGS in TBS for 48 h at 4°C.

    Techniques: Immunofluorescence, Labeling