p2x3  (Alomone Labs)


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    Alomone Labs p2x3
    Sonidegib treatment reduces taste buds (TB), SHH ligand and proliferation in rat fungiform papilla (FP) while innervation is retained. ( a ) Immunofluorescent antibody detection of SHH ligand (red) and K19 (green) for TB cells; K18 (red) for TB cells and Ki67 (green) for cell proliferation, after Vehicle, 16d or 28d Sonidegib treatments. SHH is reduced in association with TB, K19+ cell loss. Asterisks (*) indicate nonspecific SHH immunoproduct in cornified surface cells in 16d Sonidegib image. The Vehicle, K18/Ki67 image shows 3 regions positive for Ki67+ cells (Apical, Basal and Perigemmal). Proliferating cells are lost in Apical FP region after 16–28d Sonidegib. ( b ) Number of Ki67+ cells in Apical and Basal regions of FP in Vehicle- and Sonidegib-treated mice. Numbers of tongues analyzed are in parentheses. For each tongue 8–10 FP were analyzed. Sonidegib treatment reduces apical epithelial cell proliferation in FP compared to Vehicle. Statistical analysis was one-way ANOVA with Tukey HSD posthoc comparisons (*p ≤ 0.05, compared to Vehicle, APICAL). ( c ) Immunofluorescent antibody detection of K19 or K18 (red) for TB cells and NF (green) for lingual and CT innervation or <t>P2X3</t> (green) for CT nerve fibers. Innervation was retained after Sonidegib exposure. Asterisks (*) indicate nonspecific P2X3 immunoproduct in surface layer in Vehicle image. ( d ) Enlarged images from 28d Sonidegib papillae. Arrows point to NF+ or P2X3+ fibers in the FP epithelium. Throughout, white dotted lines indicate the basal lamina. Yellow dotted lines indicate surface of epithelium. ( a , c ) Scale bar: 50 μm, applies to all images. ( d ) Scale bar: 25 μm, applies to both images.
    P2x3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x3/product/Alomone Labs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    p2x3 - by Bioz Stars, 2022-11
    93/100 stars

    Images

    1) Product Images from "Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib"

    Article Title: Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-34399-3

    Sonidegib treatment reduces taste buds (TB), SHH ligand and proliferation in rat fungiform papilla (FP) while innervation is retained. ( a ) Immunofluorescent antibody detection of SHH ligand (red) and K19 (green) for TB cells; K18 (red) for TB cells and Ki67 (green) for cell proliferation, after Vehicle, 16d or 28d Sonidegib treatments. SHH is reduced in association with TB, K19+ cell loss. Asterisks (*) indicate nonspecific SHH immunoproduct in cornified surface cells in 16d Sonidegib image. The Vehicle, K18/Ki67 image shows 3 regions positive for Ki67+ cells (Apical, Basal and Perigemmal). Proliferating cells are lost in Apical FP region after 16–28d Sonidegib. ( b ) Number of Ki67+ cells in Apical and Basal regions of FP in Vehicle- and Sonidegib-treated mice. Numbers of tongues analyzed are in parentheses. For each tongue 8–10 FP were analyzed. Sonidegib treatment reduces apical epithelial cell proliferation in FP compared to Vehicle. Statistical analysis was one-way ANOVA with Tukey HSD posthoc comparisons (*p ≤ 0.05, compared to Vehicle, APICAL). ( c ) Immunofluorescent antibody detection of K19 or K18 (red) for TB cells and NF (green) for lingual and CT innervation or P2X3 (green) for CT nerve fibers. Innervation was retained after Sonidegib exposure. Asterisks (*) indicate nonspecific P2X3 immunoproduct in surface layer in Vehicle image. ( d ) Enlarged images from 28d Sonidegib papillae. Arrows point to NF+ or P2X3+ fibers in the FP epithelium. Throughout, white dotted lines indicate the basal lamina. Yellow dotted lines indicate surface of epithelium. ( a , c ) Scale bar: 50 μm, applies to all images. ( d ) Scale bar: 25 μm, applies to both images.
    Figure Legend Snippet: Sonidegib treatment reduces taste buds (TB), SHH ligand and proliferation in rat fungiform papilla (FP) while innervation is retained. ( a ) Immunofluorescent antibody detection of SHH ligand (red) and K19 (green) for TB cells; K18 (red) for TB cells and Ki67 (green) for cell proliferation, after Vehicle, 16d or 28d Sonidegib treatments. SHH is reduced in association with TB, K19+ cell loss. Asterisks (*) indicate nonspecific SHH immunoproduct in cornified surface cells in 16d Sonidegib image. The Vehicle, K18/Ki67 image shows 3 regions positive for Ki67+ cells (Apical, Basal and Perigemmal). Proliferating cells are lost in Apical FP region after 16–28d Sonidegib. ( b ) Number of Ki67+ cells in Apical and Basal regions of FP in Vehicle- and Sonidegib-treated mice. Numbers of tongues analyzed are in parentheses. For each tongue 8–10 FP were analyzed. Sonidegib treatment reduces apical epithelial cell proliferation in FP compared to Vehicle. Statistical analysis was one-way ANOVA with Tukey HSD posthoc comparisons (*p ≤ 0.05, compared to Vehicle, APICAL). ( c ) Immunofluorescent antibody detection of K19 or K18 (red) for TB cells and NF (green) for lingual and CT innervation or P2X3 (green) for CT nerve fibers. Innervation was retained after Sonidegib exposure. Asterisks (*) indicate nonspecific P2X3 immunoproduct in surface layer in Vehicle image. ( d ) Enlarged images from 28d Sonidegib papillae. Arrows point to NF+ or P2X3+ fibers in the FP epithelium. Throughout, white dotted lines indicate the basal lamina. Yellow dotted lines indicate surface of epithelium. ( a , c ) Scale bar: 50 μm, applies to all images. ( d ) Scale bar: 25 μm, applies to both images.

    Techniques Used: Mouse Assay

    Long term Sonidegib treatment in mouse reduces taste buds (TB) and SHH ligand in circumvallate papilla (CV) whereas proliferation and innervation are retained. Immunofluorescent antibody detection of SHH ligand (red) and K8 (green) for TB cells; K8 (red) for TB cells and Ki67 (green) for cell proliferation; and K8 (red) with NF (green) for GL innervation or P2X3 (green) for GL taste fibers, after Vehicle or 48d Sonidegib treatment. For SHH/K8, large dotted lines indicate the basal lamina. Small dotted lines outline the surface epithelium. Inset (K8/P2X3) shows an image of nerves extending into CV epithelial basal lamina (arrow). Scale bar: 50 μm, applies to all images. Inset at 2×.
    Figure Legend Snippet: Long term Sonidegib treatment in mouse reduces taste buds (TB) and SHH ligand in circumvallate papilla (CV) whereas proliferation and innervation are retained. Immunofluorescent antibody detection of SHH ligand (red) and K8 (green) for TB cells; K8 (red) for TB cells and Ki67 (green) for cell proliferation; and K8 (red) with NF (green) for GL innervation or P2X3 (green) for GL taste fibers, after Vehicle or 48d Sonidegib treatment. For SHH/K8, large dotted lines indicate the basal lamina. Small dotted lines outline the surface epithelium. Inset (K8/P2X3) shows an image of nerves extending into CV epithelial basal lamina (arrow). Scale bar: 50 μm, applies to all images. Inset at 2×.

    Techniques Used:

    Sonidegib treatment in rat reduces taste buds (TB) and SHH ligand in circumvallate papilla (CV) whereas cell proliferation and GL innervation are retained. Immunofluorescent antibody detection of SHH ligand (red) and K19 (green) for TB cells; K18 (red) for TB cells and Ki67 (green) for cell proliferation; K19 (red) for TB cells and NF (green) for innervation; and, K18 (red) for TB cells and P2X3 (green) for taste nerve fibers, after Vehicle or 36d Sonidegib treatment. White dotted lines outline the epithelium. Asterisk in SHH/K19, 36d Sonidegib indicates nonspecific K19 immunostaining. Arrow points to the P2X3+ nerves extending into CV epithelium after Sonidegib treatment. SHH is reduced in association with TB cells. Cell proliferation is maintained and nerves fibers are retained after Sonidegib treatment. Scale bar: 50μm, applies to all images, except K19/NF.
    Figure Legend Snippet: Sonidegib treatment in rat reduces taste buds (TB) and SHH ligand in circumvallate papilla (CV) whereas cell proliferation and GL innervation are retained. Immunofluorescent antibody detection of SHH ligand (red) and K19 (green) for TB cells; K18 (red) for TB cells and Ki67 (green) for cell proliferation; K19 (red) for TB cells and NF (green) for innervation; and, K18 (red) for TB cells and P2X3 (green) for taste nerve fibers, after Vehicle or 36d Sonidegib treatment. White dotted lines outline the epithelium. Asterisk in SHH/K19, 36d Sonidegib indicates nonspecific K19 immunostaining. Arrow points to the P2X3+ nerves extending into CV epithelium after Sonidegib treatment. SHH is reduced in association with TB cells. Cell proliferation is maintained and nerves fibers are retained after Sonidegib treatment. Scale bar: 50μm, applies to all images, except K19/NF.

    Techniques Used: Immunostaining

    2) Product Images from "Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib"

    Article Title: Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-34399-3

    Sonidegib treatment reduces taste buds (TB), SHH ligand and proliferation in rat fungiform papilla (FP) while innervation is retained. ( a ) Immunofluorescent antibody detection of SHH ligand (red) and K19 (green) for TB cells; K18 (red) for TB cells and Ki67 (green) for cell proliferation, after Vehicle, 16d or 28d Sonidegib treatments. SHH is reduced in association with TB, K19+ cell loss. Asterisks (*) indicate nonspecific SHH immunoproduct in cornified surface cells in 16d Sonidegib image. The Vehicle, K18/Ki67 image shows 3 regions positive for Ki67+ cells (Apical, Basal and Perigemmal). Proliferating cells are lost in Apical FP region after 16–28d Sonidegib. ( b ) Number of Ki67+ cells in Apical and Basal regions of FP in Vehicle- and Sonidegib-treated mice. Numbers of tongues analyzed are in parentheses. For each tongue 8–10 FP were analyzed. Sonidegib treatment reduces apical epithelial cell proliferation in FP compared to Vehicle. Statistical analysis was one-way ANOVA with Tukey HSD posthoc comparisons (*p ≤ 0.05, compared to Vehicle, APICAL). ( c ) Immunofluorescent antibody detection of K19 or K18 (red) for TB cells and NF (green) for lingual and CT innervation or P2X3 (green) for CT nerve fibers. Innervation was retained after Sonidegib exposure. Asterisks (*) indicate nonspecific P2X3 immunoproduct in surface layer in Vehicle image. ( d ) Enlarged images from 28d Sonidegib papillae. Arrows point to NF+ or P2X3+ fibers in the FP epithelium. Throughout, white dotted lines indicate the basal lamina. Yellow dotted lines indicate surface of epithelium. ( a , c ) Scale bar: 50 μm, applies to all images. ( d ) Scale bar: 25 μm, applies to both images.
    Figure Legend Snippet: Sonidegib treatment reduces taste buds (TB), SHH ligand and proliferation in rat fungiform papilla (FP) while innervation is retained. ( a ) Immunofluorescent antibody detection of SHH ligand (red) and K19 (green) for TB cells; K18 (red) for TB cells and Ki67 (green) for cell proliferation, after Vehicle, 16d or 28d Sonidegib treatments. SHH is reduced in association with TB, K19+ cell loss. Asterisks (*) indicate nonspecific SHH immunoproduct in cornified surface cells in 16d Sonidegib image. The Vehicle, K18/Ki67 image shows 3 regions positive for Ki67+ cells (Apical, Basal and Perigemmal). Proliferating cells are lost in Apical FP region after 16–28d Sonidegib. ( b ) Number of Ki67+ cells in Apical and Basal regions of FP in Vehicle- and Sonidegib-treated mice. Numbers of tongues analyzed are in parentheses. For each tongue 8–10 FP were analyzed. Sonidegib treatment reduces apical epithelial cell proliferation in FP compared to Vehicle. Statistical analysis was one-way ANOVA with Tukey HSD posthoc comparisons (*p ≤ 0.05, compared to Vehicle, APICAL). ( c ) Immunofluorescent antibody detection of K19 or K18 (red) for TB cells and NF (green) for lingual and CT innervation or P2X3 (green) for CT nerve fibers. Innervation was retained after Sonidegib exposure. Asterisks (*) indicate nonspecific P2X3 immunoproduct in surface layer in Vehicle image. ( d ) Enlarged images from 28d Sonidegib papillae. Arrows point to NF+ or P2X3+ fibers in the FP epithelium. Throughout, white dotted lines indicate the basal lamina. Yellow dotted lines indicate surface of epithelium. ( a , c ) Scale bar: 50 μm, applies to all images. ( d ) Scale bar: 25 μm, applies to both images.

    Techniques Used: Mouse Assay

    Long term Sonidegib treatment in mouse reduces taste buds (TB) and SHH ligand in circumvallate papilla (CV) whereas proliferation and innervation are retained. Immunofluorescent antibody detection of SHH ligand (red) and K8 (green) for TB cells; K8 (red) for TB cells and Ki67 (green) for cell proliferation; and K8 (red) with NF (green) for GL innervation or P2X3 (green) for GL taste fibers, after Vehicle or 48d Sonidegib treatment. For SHH/K8, large dotted lines indicate the basal lamina. Small dotted lines outline the surface epithelium. Inset (K8/P2X3) shows an image of nerves extending into CV epithelial basal lamina (arrow). Scale bar: 50 μm, applies to all images. Inset at 2×.
    Figure Legend Snippet: Long term Sonidegib treatment in mouse reduces taste buds (TB) and SHH ligand in circumvallate papilla (CV) whereas proliferation and innervation are retained. Immunofluorescent antibody detection of SHH ligand (red) and K8 (green) for TB cells; K8 (red) for TB cells and Ki67 (green) for cell proliferation; and K8 (red) with NF (green) for GL innervation or P2X3 (green) for GL taste fibers, after Vehicle or 48d Sonidegib treatment. For SHH/K8, large dotted lines indicate the basal lamina. Small dotted lines outline the surface epithelium. Inset (K8/P2X3) shows an image of nerves extending into CV epithelial basal lamina (arrow). Scale bar: 50 μm, applies to all images. Inset at 2×.

    Techniques Used:

    Sonidegib treatment in rat reduces taste buds (TB) and SHH ligand in circumvallate papilla (CV) whereas cell proliferation and GL innervation are retained. Immunofluorescent antibody detection of SHH ligand (red) and K19 (green) for TB cells; K18 (red) for TB cells and Ki67 (green) for cell proliferation; K19 (red) for TB cells and NF (green) for innervation; and, K18 (red) for TB cells and P2X3 (green) for taste nerve fibers, after Vehicle or 36d Sonidegib treatment. White dotted lines outline the epithelium. Asterisk in SHH/K19, 36d Sonidegib indicates nonspecific K19 immunostaining. Arrow points to the P2X3+ nerves extending into CV epithelium after Sonidegib treatment. SHH is reduced in association with TB cells. Cell proliferation is maintained and nerves fibers are retained after Sonidegib treatment. Scale bar: 50μm, applies to all images, except K19/NF.
    Figure Legend Snippet: Sonidegib treatment in rat reduces taste buds (TB) and SHH ligand in circumvallate papilla (CV) whereas cell proliferation and GL innervation are retained. Immunofluorescent antibody detection of SHH ligand (red) and K19 (green) for TB cells; K18 (red) for TB cells and Ki67 (green) for cell proliferation; K19 (red) for TB cells and NF (green) for innervation; and, K18 (red) for TB cells and P2X3 (green) for taste nerve fibers, after Vehicle or 36d Sonidegib treatment. White dotted lines outline the epithelium. Asterisk in SHH/K19, 36d Sonidegib indicates nonspecific K19 immunostaining. Arrow points to the P2X3+ nerves extending into CV epithelium after Sonidegib treatment. SHH is reduced in association with TB cells. Cell proliferation is maintained and nerves fibers are retained after Sonidegib treatment. Scale bar: 50μm, applies to all images, except K19/NF.

    Techniques Used: Immunostaining

    3) Product Images from "P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes"

    Article Title: P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0155107

    Expression of purinergic receptors and pannexin 1 channels is altered in T1D Akita mice bones. (A) Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X3R, P2X4R and P2X7R) and Panx1 in 8-week-old Akita and age-matched wildtype bone tissue (Data are presented as the means ± SEM *P
    Figure Legend Snippet: Expression of purinergic receptors and pannexin 1 channels is altered in T1D Akita mice bones. (A) Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X3R, P2X4R and P2X7R) and Panx1 in 8-week-old Akita and age-matched wildtype bone tissue (Data are presented as the means ± SEM *P

    Techniques Used: Expressing, Mouse Assay

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    Alomone Labs anti p2x3
    OD and ctrl DRGs showed similar proportion of <t>P2X3</t> and CGRP positive neurons. Ai , Representative brightfield (top) and anti-P2X3-antibody stained (bottom) image of a whole-sheep DRG in cross-section along with the percentage of neurons positive for P2X3 ( Aii ). Bi , Representative brightfield (top) and anti-CGRP-antibody stained (bottom) image of a whole-sheep DRG in cross-section along with the percentage of neurons positive for CGRP ( Bii ). Histograms of cross-sectional areas of neurons stained positive by anti-CGRP ( C ) and anti-P2X3 ( D ) antibodies. Numbers on the bars represent neurons positive for the respective antibody staining; * p
    Anti P2x3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p2x3/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p2x3 - by Bioz Stars, 2022-11
    93/100 stars
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    OD and ctrl DRGs showed similar proportion of P2X3 and CGRP positive neurons. Ai , Representative brightfield (top) and anti-P2X3-antibody stained (bottom) image of a whole-sheep DRG in cross-section along with the percentage of neurons positive for P2X3 ( Aii ). Bi , Representative brightfield (top) and anti-CGRP-antibody stained (bottom) image of a whole-sheep DRG in cross-section along with the percentage of neurons positive for CGRP ( Bii ). Histograms of cross-sectional areas of neurons stained positive by anti-CGRP ( C ) and anti-P2X3 ( D ) antibodies. Numbers on the bars represent neurons positive for the respective antibody staining; * p

    Journal: eNeuro

    Article Title: Functional Characterization of Ovine Dorsal Root Ganglion Neurons Reveal Peripheral Sensitization after Osteochondral Defect

    doi: 10.1523/ENEURO.0237-21.2021

    Figure Lengend Snippet: OD and ctrl DRGs showed similar proportion of P2X3 and CGRP positive neurons. Ai , Representative brightfield (top) and anti-P2X3-antibody stained (bottom) image of a whole-sheep DRG in cross-section along with the percentage of neurons positive for P2X3 ( Aii ). Bi , Representative brightfield (top) and anti-CGRP-antibody stained (bottom) image of a whole-sheep DRG in cross-section along with the percentage of neurons positive for CGRP ( Bii ). Histograms of cross-sectional areas of neurons stained positive by anti-CGRP ( C ) and anti-P2X3 ( D ) antibodies. Numbers on the bars represent neurons positive for the respective antibody staining; * p

    Article Snippet: Anti-CGRP (1:5000, Sigma C8189, anti-rabbit polyclonal) and anti-P2X3 (1:1000, Alomone APR016, anti-rabbit polyclonal) primary antibodies were used in combination with an Alexa Fluor 488 anti-rabbit conjugated secondary antibody (1:1000, Invitrogen A21206) and Alexa Fluor 568 anti-mouse conjugated secondary antibody (1:500, Invitrogen A-11031).

    Techniques: Staining

    A) Histogram showing area of each sheep DRG neuron imaged from whole DRG sections and the criteria used in this article for assigning neurons into small, medium and large category. B) Histogram of neuronal diameters on which whole-cell patch clamp was performed. Histograms of cross-sectional areas of neurons stained positive by anti-CGRP (C) and anti-P2X3 (D) antibodies.

    Journal: bioRxiv

    Article Title: Functional characterization of ovine dorsal root ganglion neurons reveals peripheral sensitization after osteochondral defect

    doi: 10.1101/2021.02.26.432434

    Figure Lengend Snippet: A) Histogram showing area of each sheep DRG neuron imaged from whole DRG sections and the criteria used in this article for assigning neurons into small, medium and large category. B) Histogram of neuronal diameters on which whole-cell patch clamp was performed. Histograms of cross-sectional areas of neurons stained positive by anti-CGRP (C) and anti-P2X3 (D) antibodies.

    Article Snippet: Anti-CGRP antibody (1:5000, Sigma C8189, anti-rabbit polyclonal), and anti-P2X3 antibody (1:1000, Alomone APR016, anti-rabbit polyclonal) following Alexa-488 conjugated secondary antibody (1:1000, Invitrogen A21206, anti-rabbit) and Alexa-568 conjugated secondary antibody (1:500, Invitrogen A-11031, anti-mouse) were used in staining.

    Techniques: Patch Clamp, Staining

    A) Patch-clamp traces showing a representative DRG neuron from the ctrl side firing AP spikes above baseline and same as baseline in response to capsaicin and α,β me-ATP respectively. B) Bar graph showing percentage of neurons with above baseline firing activity in response to capsaicin (left, orange) and α,β me-ATP (right, green). Numbers on the bars represent number of neurons in each condition firing above baseline. Ci) (top) Representative Ca 2+ trace from a neuron responding to capsaicin and KCl (positive control). (bottom) Magnitude of Ca 2+ influx in response to capsaicin (ctrl, n = 35, defect, n = 48). Cii) Percentage of neurons responding to capsaicin in each condition. Di) (top) Representative Ca 2+ trace from a neuron responding to α,β me-ATP and KCl (positive control), (bottom) magnitude of Ca 2+ influx in response to α,β me-ATP (ctrl, n = 24, defect, n = 23). Dii) Percentage of neurons responding to α,β me-ATP in each condition. Ei) Representative brightfield (top) and anti-P2X3-antibody stained (bottom) image of a whole sheep DRG in cross-section along with the percentage of neurons positive for P2X3 (Eii). Fi) Representative brightfield (top) and anti-CGRP-antibody stained (bottom) image of a whole sheep DRG in cross-section along with the percentage of neurons positive for CGRP (Fii). Numbers on the bars represent neurons positive for the respective antibody staining. *: p

    Journal: bioRxiv

    Article Title: Functional characterization of ovine dorsal root ganglion neurons reveals peripheral sensitization after osteochondral defect

    doi: 10.1101/2021.02.26.432434

    Figure Lengend Snippet: A) Patch-clamp traces showing a representative DRG neuron from the ctrl side firing AP spikes above baseline and same as baseline in response to capsaicin and α,β me-ATP respectively. B) Bar graph showing percentage of neurons with above baseline firing activity in response to capsaicin (left, orange) and α,β me-ATP (right, green). Numbers on the bars represent number of neurons in each condition firing above baseline. Ci) (top) Representative Ca 2+ trace from a neuron responding to capsaicin and KCl (positive control). (bottom) Magnitude of Ca 2+ influx in response to capsaicin (ctrl, n = 35, defect, n = 48). Cii) Percentage of neurons responding to capsaicin in each condition. Di) (top) Representative Ca 2+ trace from a neuron responding to α,β me-ATP and KCl (positive control), (bottom) magnitude of Ca 2+ influx in response to α,β me-ATP (ctrl, n = 24, defect, n = 23). Dii) Percentage of neurons responding to α,β me-ATP in each condition. Ei) Representative brightfield (top) and anti-P2X3-antibody stained (bottom) image of a whole sheep DRG in cross-section along with the percentage of neurons positive for P2X3 (Eii). Fi) Representative brightfield (top) and anti-CGRP-antibody stained (bottom) image of a whole sheep DRG in cross-section along with the percentage of neurons positive for CGRP (Fii). Numbers on the bars represent neurons positive for the respective antibody staining. *: p

    Article Snippet: Anti-CGRP antibody (1:5000, Sigma C8189, anti-rabbit polyclonal), and anti-P2X3 antibody (1:1000, Alomone APR016, anti-rabbit polyclonal) following Alexa-488 conjugated secondary antibody (1:1000, Invitrogen A21206, anti-rabbit) and Alexa-568 conjugated secondary antibody (1:500, Invitrogen A-11031, anti-mouse) were used in staining.

    Techniques: Patch Clamp, Activity Assay, Positive Control, Staining