p2y12  (Alomone Labs)


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    Name:
    Anti P2Y12 Receptor Antibody
    Description:
    Anti P2Y12 Receptor Antibody APR 012 is a highly specific antibody directed against an intracellular epitope of the human P2RY12 The antibody can be used in western blot immunoprecipitation indirect flow cytometry immunohistochemistry and immunocytochemistry applications It has been designed to recognize P2RY12 from human rat and mouse samples
    Catalog Number:
    APR-012
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs p2y12
    Anti P2Y12 Receptor Antibody
    Anti P2Y12 Receptor Antibody APR 012 is a highly specific antibody directed against an intracellular epitope of the human P2RY12 The antibody can be used in western blot immunoprecipitation indirect flow cytometry immunohistochemistry and immunocytochemistry applications It has been designed to recognize P2RY12 from human rat and mouse samples
    https://www.bioz.com/result/p2y12/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2y12 - by Bioz Stars, 2021-09
    94/100 stars

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    Related Articles

    other:

    Article Title: The Impact of Cold Storage on Adenosine Diphosphate-Mediated Platelet Responsiveness
    Article Snippet: Rabbit polyclonal anti-P2Y1, anti-P2Y12, and anti-P2X1 antibodies were from Alomone Labs (Jerusalem, Israel).

    Article Title: ASK1 facilitates tumor metastasis through phosphorylation of an ADP receptor P2Y12 in platelets
    Article Snippet: The antibody to ASK1 (EP553Y) was purchased from Abcam (Cambridge, UK) and the antibody to P2Y12 (APR-012) was purchased from Alomone Labs (Jerusalem, Israel).

    Incubation:

    Article Title: Long Non-coding RNA Uc.48+ Small Interfering RNA Alleviates Neuroinflammatory Hyperalgesia in Gp120-Treated Rats via the P2Y12 Receptor
    Article Snippet: .. The membrane was blocked for 2 h, then incubated with rabbit anti-P2Y12 (1:2,000 dilutions, Alomone Labs, Israel), rabbit anti-p38 mitogen-activated protein kinase (p38 MAPK), rabbit anti-phosphorylated-p38 (P-p38 MAPK) (1:1,000 dilutions, Cell Signaling Technology, United States), and mouse monoclonal anti-β-actin (1:800 dilutions, ZSGB-Bio) antibodies at 4°C overnight. ..

    Article Title: P2Y12 receptor upregulation in satellite glial cells is involved in neuropathic pain induced by HIV glycoprotein 120 and 2′,3′-dideoxycytidine
    Article Snippet: .. The sections were then incubated with rabbit anti-P2Y12 receptor (1:50, Alomone Lab, Israel) and mouse anti-glial fibrillary acidic protein (GFAP) (1:150, Millipore, USA) overnight at 4 °C. ..

    Purification:

    Article Title: P2Y13 receptors mediate presynaptic inhibition of acetylcholine release induced by adenine nucleotides at the mouse neuromuscular junction.
    Article Snippet: .. It is known that adenosine 5'-triphosphate (ATP) is released along with the neurotransmitter acetylcholine (ACh) from motor nerve terminals.. At mammalian neuromuscular junctions (NMJs), we have previously demonstrated that ATP is able to decrease ACh secretion by activation of P2Y receptors coupled to pertussis toxin-sensitive Gi/o protein. ..

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  • 94
    Alomone Labs anti p2y12
    Expression and function of purinergic receptors. The mean fluorescence intensities (MFI) of basal or 10 μM TRAP-6-stimulated receptor expression, detected by flow cytometry, are shown for P2Y1 ( A ), <t>P2Y12</t> ( B ) and P2X1 ( C ). The function of the P2Y1 ( D ) and of the P2X1 ( E ) receptor was measured by calcium-induced fluorescence after selective stimulation, shown as mean values in relative fluorescence units (RFU). The function of the P2Y12 receptor ( F ) is expressed as platelet reactivity index (PRI) determined by the flow cytometric VASP assay. Mean ± SEM; n = 6; * p
    Anti P2y12, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p2y12/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p2y12 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Expression and function of purinergic receptors. The mean fluorescence intensities (MFI) of basal or 10 μM TRAP-6-stimulated receptor expression, detected by flow cytometry, are shown for P2Y1 ( A ), P2Y12 ( B ) and P2X1 ( C ). The function of the P2Y1 ( D ) and of the P2X1 ( E ) receptor was measured by calcium-induced fluorescence after selective stimulation, shown as mean values in relative fluorescence units (RFU). The function of the P2Y12 receptor ( F ) is expressed as platelet reactivity index (PRI) determined by the flow cytometric VASP assay. Mean ± SEM; n = 6; * p

    Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

    Article Title: The Impact of Cold Storage on Adenosine Diphosphate-Mediated Platelet Responsiveness

    doi: 10.1055/s-0040-1714254

    Figure Lengend Snippet: Expression and function of purinergic receptors. The mean fluorescence intensities (MFI) of basal or 10 μM TRAP-6-stimulated receptor expression, detected by flow cytometry, are shown for P2Y1 ( A ), P2Y12 ( B ) and P2X1 ( C ). The function of the P2Y1 ( D ) and of the P2X1 ( E ) receptor was measured by calcium-induced fluorescence after selective stimulation, shown as mean values in relative fluorescence units (RFU). The function of the P2Y12 receptor ( F ) is expressed as platelet reactivity index (PRI) determined by the flow cytometric VASP assay. Mean ± SEM; n = 6; * p

    Article Snippet: Rabbit polyclonal anti-P2Y1, anti-P2Y12, and anti-P2X1 antibodies were from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Fluorescence, Flow Cytometry

    The activity of platelet P2Y1, but not of P2X1 or P2Y12 receptor, continually decreases in citrated WB. Calcium induced fluorescence curves were generated with Fluo-4AM loaded freshly washed platelets from PB samples (black line) and from WB units on Day 0 (dashed line), on Day 2 (dotted line) and on Day 5 (gray line) after stimulation with the P2Y1 agonist MRS2365 (A) or the P2X1 agonist α,β-MeATP (C). Mean fluorescence curves are presented (expressed in relative fluorescence units, R.F.U.); n = 6. For the P2Y12 receptor activity, mean PRI values (B) were determined in PB samples and in stored WB units at different time points as indicated. Results are presented as mean % (PRI) ± SEM; n = 4.

    Journal: PLoS ONE

    Article Title: The role of adenosine diphosphate mediated platelet responsiveness for the stability of platelet integrity in citrated whole blood under ex vivo conditions

    doi: 10.1371/journal.pone.0188193

    Figure Lengend Snippet: The activity of platelet P2Y1, but not of P2X1 or P2Y12 receptor, continually decreases in citrated WB. Calcium induced fluorescence curves were generated with Fluo-4AM loaded freshly washed platelets from PB samples (black line) and from WB units on Day 0 (dashed line), on Day 2 (dotted line) and on Day 5 (gray line) after stimulation with the P2Y1 agonist MRS2365 (A) or the P2X1 agonist α,β-MeATP (C). Mean fluorescence curves are presented (expressed in relative fluorescence units, R.F.U.); n = 6. For the P2Y12 receptor activity, mean PRI values (B) were determined in PB samples and in stored WB units at different time points as indicated. Results are presented as mean % (PRI) ± SEM; n = 4.

    Article Snippet: Rabbit polyclonal anti-P2Y1, anti-P2Y12 and anti-P2X1 antibodies were from Alomone Labs (Jerusalem, Israel).

    Techniques: Activity Assay, Western Blot, Fluorescence, Generated

    Platelet purinergic receptor expression in citrated WB is maintained during storage. The histograms show the mean fluorescence intensities (MFI) of basal (white columns) and 10 μM TRAP-6 stimulated (shaded columns) P2Y1- (A), P2Y12- (B), and P2X1- (C) receptor expression in fresh PB samples and in stored WB units at different time points as indicated. Results are presented in absolute arbitrary units (AU) as mean fluorescence ± SEM; n = 6; *: p

    Journal: PLoS ONE

    Article Title: The role of adenosine diphosphate mediated platelet responsiveness for the stability of platelet integrity in citrated whole blood under ex vivo conditions

    doi: 10.1371/journal.pone.0188193

    Figure Lengend Snippet: Platelet purinergic receptor expression in citrated WB is maintained during storage. The histograms show the mean fluorescence intensities (MFI) of basal (white columns) and 10 μM TRAP-6 stimulated (shaded columns) P2Y1- (A), P2Y12- (B), and P2X1- (C) receptor expression in fresh PB samples and in stored WB units at different time points as indicated. Results are presented in absolute arbitrary units (AU) as mean fluorescence ± SEM; n = 6; *: p

    Article Snippet: Rabbit polyclonal anti-P2Y1, anti-P2Y12 and anti-P2X1 antibodies were from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Western Blot, Fluorescence

    P2X4, P2X7 and P2Y12 immunoexpression in AMC in postnatal rat brain . By immunofluorescence labeling, P2X4 is colocalized with OX42 in the amoeboid microglial cells. Colocalization of OX42 and P2X4 in cells (arrows) is seen in the corpus callosum and subventricular zone (Aa-c, Scale bar = 100 μm). Immunopositive cells are round and have a typical morphology of amoeboid microglial cells (B/Ca-c. Scale bar = 20 μm). The amoeboid microglial cells exhibit a stronger P2X4 immunoreactivity compared with that of P2X7 and P2Y12 (D/Ea-c, Scale bar = 20 μm.).

    Journal: BMC Neuroscience

    Article Title: Hypoxia induced amoeboid microglial cell activation in postnatal rat brain is mediated by ATP receptor P2X4

    doi: 10.1186/1471-2202-12-111

    Figure Lengend Snippet: P2X4, P2X7 and P2Y12 immunoexpression in AMC in postnatal rat brain . By immunofluorescence labeling, P2X4 is colocalized with OX42 in the amoeboid microglial cells. Colocalization of OX42 and P2X4 in cells (arrows) is seen in the corpus callosum and subventricular zone (Aa-c, Scale bar = 100 μm). Immunopositive cells are round and have a typical morphology of amoeboid microglial cells (B/Ca-c. Scale bar = 20 μm). The amoeboid microglial cells exhibit a stronger P2X4 immunoreactivity compared with that of P2X7 and P2Y12 (D/Ea-c, Scale bar = 20 μm.).

    Article Snippet: The sections were then incubated at 22-24°C with rabbit anti-P2X4 polyclonal primary antibody (1: 200, Alomone, Cat: APR-002, Yiselie), rabbit anti-P2X7 polyclonal primary antibody (1: 200, Alomone, Cat: APR-004, Yiselie), and rabbit anti-P2Y12 polyclonal primary antibody (1: 200, Alomone, Cat: APR-012, Yiselie) overnight.

    Techniques: Immunofluorescence, Labeling

    Overexpression of P2Y12 rescued effects of uc.48+ siRNA treatment on downregulated expression of P2Y12 receptor and hyperalgesia in gp120 group. (A) Western blotting results showed that downregulation of DRG P2Y12 protein in gp120-treated rats with uc.48+ siRNA was significantly rescued by overexpression of P2Y12. (B,C) Upregulation of MWT and TWL was rescued by overexpression of P2Y12. n = 10 rats per group. Data are displayed as means ± SEM. ∗∗ p

    Journal: Frontiers in Neuroscience

    Article Title: Long Non-coding RNA Uc.48+ Small Interfering RNA Alleviates Neuroinflammatory Hyperalgesia in Gp120-Treated Rats via the P2Y12 Receptor

    doi: 10.3389/fnins.2021.663962

    Figure Lengend Snippet: Overexpression of P2Y12 rescued effects of uc.48+ siRNA treatment on downregulated expression of P2Y12 receptor and hyperalgesia in gp120 group. (A) Western blotting results showed that downregulation of DRG P2Y12 protein in gp120-treated rats with uc.48+ siRNA was significantly rescued by overexpression of P2Y12. (B,C) Upregulation of MWT and TWL was rescued by overexpression of P2Y12. n = 10 rats per group. Data are displayed as means ± SEM. ∗∗ p

    Article Snippet: The membrane was blocked for 2 h, then incubated with rabbit anti-P2Y12 (1:2,000 dilutions, Alomone Labs, Israel), rabbit anti-p38 mitogen-activated protein kinase (p38 MAPK), rabbit anti-phosphorylated-p38 (P-p38 MAPK) (1:1,000 dilutions, Cell Signaling Technology, United States), and mouse monoclonal anti-β-actin (1:800 dilutions, ZSGB-Bio) antibodies at 4°C overnight.

    Techniques: Over Expression, Expressing, Western Blot

    Effects of uc.48+ on P2Y12 receptor expression and activation of P2Y12 downstream P38 MAPK pathway in vivo . Real-time PCR (A) and Western blotting (B) analyses showed siRNA silencing of uc.48+ downregulated P2Y12 receptor expression. n = 10 rats per group. Data are displayed as means ± SEM. ∗∗ p

    Journal: Frontiers in Neuroscience

    Article Title: Long Non-coding RNA Uc.48+ Small Interfering RNA Alleviates Neuroinflammatory Hyperalgesia in Gp120-Treated Rats via the P2Y12 Receptor

    doi: 10.3389/fnins.2021.663962

    Figure Lengend Snippet: Effects of uc.48+ on P2Y12 receptor expression and activation of P2Y12 downstream P38 MAPK pathway in vivo . Real-time PCR (A) and Western blotting (B) analyses showed siRNA silencing of uc.48+ downregulated P2Y12 receptor expression. n = 10 rats per group. Data are displayed as means ± SEM. ∗∗ p

    Article Snippet: The membrane was blocked for 2 h, then incubated with rabbit anti-P2Y12 (1:2,000 dilutions, Alomone Labs, Israel), rabbit anti-p38 mitogen-activated protein kinase (p38 MAPK), rabbit anti-phosphorylated-p38 (P-p38 MAPK) (1:1,000 dilutions, Cell Signaling Technology, United States), and mouse monoclonal anti-β-actin (1:800 dilutions, ZSGB-Bio) antibodies at 4°C overnight.

    Techniques: Expressing, Activation Assay, In Vivo, Real-time Polymerase Chain Reaction, Western Blot

    LncRNA uc.48+ positively regulated expression and activity of P2Y12 receptor. (A) P2Y12 protein content co-transfected with P2Y12 and uc.48+ plasmids was increased compared with transfected with P2Y12 plasmid alone in HEK293 cells. (B) P2Y12 protein content co-transfected with P2Y12 plasmid and uc.48+ siRNA was decreased in HEK293 cells. n = 6 independent cultures. Data are displayed as means ± SEM. ∗∗ p

    Journal: Frontiers in Neuroscience

    Article Title: Long Non-coding RNA Uc.48+ Small Interfering RNA Alleviates Neuroinflammatory Hyperalgesia in Gp120-Treated Rats via the P2Y12 Receptor

    doi: 10.3389/fnins.2021.663962

    Figure Lengend Snippet: LncRNA uc.48+ positively regulated expression and activity of P2Y12 receptor. (A) P2Y12 protein content co-transfected with P2Y12 and uc.48+ plasmids was increased compared with transfected with P2Y12 plasmid alone in HEK293 cells. (B) P2Y12 protein content co-transfected with P2Y12 plasmid and uc.48+ siRNA was decreased in HEK293 cells. n = 6 independent cultures. Data are displayed as means ± SEM. ∗∗ p

    Article Snippet: The membrane was blocked for 2 h, then incubated with rabbit anti-P2Y12 (1:2,000 dilutions, Alomone Labs, Israel), rabbit anti-p38 mitogen-activated protein kinase (p38 MAPK), rabbit anti-phosphorylated-p38 (P-p38 MAPK) (1:1,000 dilutions, Cell Signaling Technology, United States), and mouse monoclonal anti-β-actin (1:800 dilutions, ZSGB-Bio) antibodies at 4°C overnight.

    Techniques: Expressing, Activity Assay, Transfection, Plasmid Preparation