p2y2  (Alomone Labs)


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    Structured Review

    Alomone Labs p2y2
    RT-PCR and sequence analysis of porcine <t>P2Y2</t> , P2Y4 and P2Y6 receptors
    P2y2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2y2/product/Alomone Labs
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    p2y2 - by Bioz Stars, 2022-09
    93/100 stars

    Images

    1) Product Images from "Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries"

    Article Title: Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries

    Journal:

    doi: 10.1038/sj.bjp.0707120

    RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors
    Figure Legend Snippet: RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Sequencing

    RT-PCR and immunoblot analysis of P2Y receptor expression in pig coronary and ear arteries. Agarose gel electrophoresis ( a–c ) showing size marker (MW) and PCR products from coronary (PCA) and ear (PEA) arteries or no template (CON) for p2ry2 (
    Figure Legend Snippet: RT-PCR and immunoblot analysis of P2Y receptor expression in pig coronary and ear arteries. Agarose gel electrophoresis ( a–c ) showing size marker (MW) and PCR products from coronary (PCA) and ear (PEA) arteries or no template (CON) for p2ry2 (

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis, Marker, Polymerase Chain Reaction

    2) Product Images from "Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer"

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer

    Journal: Purinergic Signalling

    doi: 10.1007/s11302-011-9228-9

    Purinergic studies in human CRC. Protein expression levels of CD39, P2X7 and P2Y2 ( a – c ) and localization of P2Y2 ( d , e ) was determined in human CRC. Changes in protein levels were observed for P2Y2 ( c ), which was significantly less in T3N + M0
    Figure Legend Snippet: Purinergic studies in human CRC. Protein expression levels of CD39, P2X7 and P2Y2 ( a – c ) and localization of P2Y2 ( d , e ) was determined in human CRC. Changes in protein levels were observed for P2Y2 ( c ), which was significantly less in T3N + M0

    Techniques Used: Expressing

    CD39 and P2 receptors in CRC. a In orthotopic tumors, CD39, P2X7 and P2Y2 mRNA appeared at comparable levels to those seen in control livers. b As expected, mouse CD39 mRNA was significantly lower expressed in genetically deficient over wild-type and
    Figure Legend Snippet: CD39 and P2 receptors in CRC. a In orthotopic tumors, CD39, P2X7 and P2Y2 mRNA appeared at comparable levels to those seen in control livers. b As expected, mouse CD39 mRNA was significantly lower expressed in genetically deficient over wild-type and

    Techniques Used:

    3) Product Images from "Nitric Oxide-mediated Modulation of Synaptic Activity by Astrocytic P2Y Receptors"

    Article Title: Nitric Oxide-mediated Modulation of Synaptic Activity by Astrocytic P2Y Receptors

    Journal: The Journal of General Physiology

    doi: 10.1085/jgp.200810043

    Representative traces of [Ca 2+ ] i response in solitary neurons and astrocytes in low density cultures stimulated with 30 μM UTP (A); 100 μM ATP (B). The traces shown are from a single neuron and astrocyte representing 15 neurons and 40 astrocytes from eight experiments. (C and D) Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and synaptophysin (red). P2Y2 and P2Y4 receptors are expressed only in astrocytes. P2Y2 and P2Y4 receptor immunofluorescence was not colocalized with synaptophysin fluorescence at synaptic terminals.
    Figure Legend Snippet: Representative traces of [Ca 2+ ] i response in solitary neurons and astrocytes in low density cultures stimulated with 30 μM UTP (A); 100 μM ATP (B). The traces shown are from a single neuron and astrocyte representing 15 neurons and 40 astrocytes from eight experiments. (C and D) Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and synaptophysin (red). P2Y2 and P2Y4 receptors are expressed only in astrocytes. P2Y2 and P2Y4 receptor immunofluorescence was not colocalized with synaptophysin fluorescence at synaptic terminals.

    Techniques Used: Immunofluorescence, Fluorescence

    Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and neuronal markers anti-MAP-2 (red). Both P2Y2 and P2Y4 receptor immunostaining is not seen in neuronal cell bodies or processes but astrocytes are profusely stained. In merged images, some pixels appear yellow because of the neurons (red) overlying the brightly stained (green) astrocytes.
    Figure Legend Snippet: Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and neuronal markers anti-MAP-2 (red). Both P2Y2 and P2Y4 receptor immunostaining is not seen in neuronal cell bodies or processes but astrocytes are profusely stained. In merged images, some pixels appear yellow because of the neurons (red) overlying the brightly stained (green) astrocytes.

    Techniques Used: Immunostaining, Staining

    4) Product Images from "Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer"

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer

    Journal: Purinergic Signalling

    doi: 10.1007/s11302-011-9228-9

    Purinergic studies in human CRC. Protein expression levels of CD39, P2X7 and P2Y2 ( a – c ) and localization of P2Y2 ( d , e ) was determined in human CRC. Changes in protein levels were observed for P2Y2 ( c ), which was significantly less in T3N + M0
    Figure Legend Snippet: Purinergic studies in human CRC. Protein expression levels of CD39, P2X7 and P2Y2 ( a – c ) and localization of P2Y2 ( d , e ) was determined in human CRC. Changes in protein levels were observed for P2Y2 ( c ), which was significantly less in T3N + M0

    Techniques Used: Expressing

    CD39 and P2 receptors in CRC. a In orthotopic tumors, CD39, P2X7 and P2Y2 mRNA appeared at comparable levels to those seen in control livers. b As expected, mouse CD39 mRNA was significantly lower expressed in genetically deficient over wild-type and
    Figure Legend Snippet: CD39 and P2 receptors in CRC. a In orthotopic tumors, CD39, P2X7 and P2Y2 mRNA appeared at comparable levels to those seen in control livers. b As expected, mouse CD39 mRNA was significantly lower expressed in genetically deficient over wild-type and

    Techniques Used:

    5) Product Images from "Primary cilia mediate mechanotransduction through control of ATP-induced Ca2+ signaling in compressed chondrocytes"

    Article Title: Primary cilia mediate mechanotransduction through control of ATP-induced Ca2+ signaling in compressed chondrocytes

    Journal: The FASEB Journal

    doi: 10.1096/fj.11-193649

    P2, PC1, and PC2 expression in WT and ORPK cells. A ) Western blots of P2 receptors, P2X4, P2X7, P2Y1, and P2Y2. B ) Quantification of P2 receptor expression reveals no significant differences between WT and ORPK chondrocytes. Data were normalized to β-actin.
    Figure Legend Snippet: P2, PC1, and PC2 expression in WT and ORPK cells. A ) Western blots of P2 receptors, P2X4, P2X7, P2Y1, and P2Y2. B ) Quantification of P2 receptor expression reveals no significant differences between WT and ORPK chondrocytes. Data were normalized to β-actin.

    Techniques Used: Expressing, Western Blot

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    Alomone Labs p2y2
    RT-PCR and sequence analysis of porcine <t>P2Y2</t> , P2Y4 and P2Y6 receptors
    P2y2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2y2/product/Alomone Labs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    p2y2 - by Bioz Stars, 2022-09
    93/100 stars
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    Image Search Results


    RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors

    Journal:

    Article Title: Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries

    doi: 10.1038/sj.bjp.0707120

    Figure Lengend Snippet: RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors

    Article Snippet: Antibodies directed against sequence-specific peptides from P2Y2 , P2Y4 and P2Y6 receptors were from Alomone Labs/Caltag-Medsystems (Buckingham, UK), while HRP-conjugated goat anti-rabbit antibody was from DAKO/Cytomation (Ely, UK).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing

    RT-PCR and immunoblot analysis of P2Y receptor expression in pig coronary and ear arteries. Agarose gel electrophoresis ( a–c ) showing size marker (MW) and PCR products from coronary (PCA) and ear (PEA) arteries or no template (CON) for p2ry2 (

    Journal:

    Article Title: Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries

    doi: 10.1038/sj.bjp.0707120

    Figure Lengend Snippet: RT-PCR and immunoblot analysis of P2Y receptor expression in pig coronary and ear arteries. Agarose gel electrophoresis ( a–c ) showing size marker (MW) and PCR products from coronary (PCA) and ear (PEA) arteries or no template (CON) for p2ry2 (

    Article Snippet: Antibodies directed against sequence-specific peptides from P2Y2 , P2Y4 and P2Y6 receptors were from Alomone Labs/Caltag-Medsystems (Buckingham, UK), while HRP-conjugated goat anti-rabbit antibody was from DAKO/Cytomation (Ely, UK).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis, Marker, Polymerase Chain Reaction

    Subcellular distribution of P2Y2, P2Y4, and P2Y6 receptors in MEFs. WT or KI MEFs were cultured and, after changing the medium, were maintained in the absence or presence of 1 μ M DFU for 2 h. Cells were fixed with paraformaldehyde (4%; pH 7.2) and permeabilized with cold methanol at RT. After incubation with anti-P2Y2, anti-P2Y4 and anti-P2Y6 antibodies (1 : 500) overnight at 4°C, cells were visualized by confocal microscopy using a FITC-conjugated secondary Ab (Alexa-Fluor 488, 1 : 1000). Nuclei were stained with Hoechst 33258. Coverslips were mounted in Prolong Gold antifade reagent (Molecular Probes) and the intensity of the fluorescence was measured using Image J software (NIH, Bethesda, MD, USA). Results show the mean + SD of three experiments. ** P

    Journal: Mediators of Inflammation

    Article Title: Sustained Release of Prostaglandin E2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca2+-Mobilization

    doi: 10.1155/2014/832103

    Figure Lengend Snippet: Subcellular distribution of P2Y2, P2Y4, and P2Y6 receptors in MEFs. WT or KI MEFs were cultured and, after changing the medium, were maintained in the absence or presence of 1 μ M DFU for 2 h. Cells were fixed with paraformaldehyde (4%; pH 7.2) and permeabilized with cold methanol at RT. After incubation with anti-P2Y2, anti-P2Y4 and anti-P2Y6 antibodies (1 : 500) overnight at 4°C, cells were visualized by confocal microscopy using a FITC-conjugated secondary Ab (Alexa-Fluor 488, 1 : 1000). Nuclei were stained with Hoechst 33258. Coverslips were mounted in Prolong Gold antifade reagent (Molecular Probes) and the intensity of the fluorescence was measured using Image J software (NIH, Bethesda, MD, USA). Results show the mean + SD of three experiments. ** P

    Article Snippet: Antibodies against P2Y2, P2Y4, and P2Y6 receptors were from Alomone Labs (Jerusalem, Israel) and other antibodies were from Santa Cruz Biotech (Santa Cruz, CA, USA), from Cell Signaling (Danvers, MA, USA), or from the sources previously described [ ].

    Techniques: Cell Culture, Incubation, Confocal Microscopy, Staining, Fluorescence, Software

    Characterization of EP1–4 and P2Y2–P2Y4 expression and effect of ionophores on Ca 2+ -mobilization in MEF cells. The expression levels of the prostaglandin receptors EP1–4 and the levels of P2Y2 and P2Y4 were determined by qPCR (a-b). The response to 1 μ M ionomycin (c) and 500 nM thapsigargin (d) on Ca 2+ -mobilization was determined in MEFs overexpressing COX-2, using the dual excitation 340/380 nm protocol as described in Section 2 . MEFs KI were washed with fresh medium to remove PGE 2 accumulated and maintained in the absence or presence of 1 μ M DFU and 5 μ M PGE 2 . Different extracellular concentrations of calcium were used. Results show the mean + SD of three experiments (a-b) or a representative trace (c-d). * P

    Journal: Mediators of Inflammation

    Article Title: Sustained Release of Prostaglandin E2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca2+-Mobilization

    doi: 10.1155/2014/832103

    Figure Lengend Snippet: Characterization of EP1–4 and P2Y2–P2Y4 expression and effect of ionophores on Ca 2+ -mobilization in MEF cells. The expression levels of the prostaglandin receptors EP1–4 and the levels of P2Y2 and P2Y4 were determined by qPCR (a-b). The response to 1 μ M ionomycin (c) and 500 nM thapsigargin (d) on Ca 2+ -mobilization was determined in MEFs overexpressing COX-2, using the dual excitation 340/380 nm protocol as described in Section 2 . MEFs KI were washed with fresh medium to remove PGE 2 accumulated and maintained in the absence or presence of 1 μ M DFU and 5 μ M PGE 2 . Different extracellular concentrations of calcium were used. Results show the mean + SD of three experiments (a-b) or a representative trace (c-d). * P

    Article Snippet: Antibodies against P2Y2, P2Y4, and P2Y6 receptors were from Alomone Labs (Jerusalem, Israel) and other antibodies were from Santa Cruz Biotech (Santa Cruz, CA, USA), from Cell Signaling (Danvers, MA, USA), or from the sources previously described [ ].

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Purinergic studies in human CRC. Protein expression levels of CD39, P2X7 and P2Y2 ( a – c ) and localization of P2Y2 ( d , e ) was determined in human CRC. Changes in protein levels were observed for P2Y2 ( c ), which was significantly less in T3N + M0

    Journal: Purinergic Signalling

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer

    doi: 10.1007/s11302-011-9228-9

    Figure Lengend Snippet: Purinergic studies in human CRC. Protein expression levels of CD39, P2X7 and P2Y2 ( a – c ) and localization of P2Y2 ( d , e ) was determined in human CRC. Changes in protein levels were observed for P2Y2 ( c ), which was significantly less in T3N + M0

    Article Snippet: To validate P2 receptor protein expression (P2X7, P2Y2), primary antibodies against P2X7 and P2Y2 were blocked with the specific antigen, as provided by the manufacturer (Alomone labs).

    Techniques: Expressing

    CD39 and P2 receptors in CRC. a In orthotopic tumors, CD39, P2X7 and P2Y2 mRNA appeared at comparable levels to those seen in control livers. b As expected, mouse CD39 mRNA was significantly lower expressed in genetically deficient over wild-type and

    Journal: Purinergic Signalling

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer

    doi: 10.1007/s11302-011-9228-9

    Figure Lengend Snippet: CD39 and P2 receptors in CRC. a In orthotopic tumors, CD39, P2X7 and P2Y2 mRNA appeared at comparable levels to those seen in control livers. b As expected, mouse CD39 mRNA was significantly lower expressed in genetically deficient over wild-type and

    Article Snippet: To validate P2 receptor protein expression (P2X7, P2Y2), primary antibodies against P2X7 and P2Y2 were blocked with the specific antigen, as provided by the manufacturer (Alomone labs).

    Techniques:

    a Fold change in expression of P2Y2 mRNA in brain and lung tissue ( n = 3) as well as calvarial osteoblasts ( n = 4) of P2Y2R-Tg rats relative to Wt ( n = 3 (brain and lung); n = 6 (calvarial osteoblasts)). b P2Y2R protein expression in calvarial osteoblasts from WT ( n = 4) and P2Y2R-Tg ( n = 4) rats as measured by Western blotting. All bands are normalized to histone H3 expression, and mean expression in Wt cells is set at 100%. Lower panel shows membrane images of each individual experiment. Error bars represent SEM. TG P2Y2R transgenic, Wt wild-type

    Journal: Purinergic Signalling

    Article Title: Bone turnover is altered in transgenic rats overexpressing the P2Y2 purinergic receptor

    doi: 10.1007/s11302-017-9582-3

    Figure Lengend Snippet: a Fold change in expression of P2Y2 mRNA in brain and lung tissue ( n = 3) as well as calvarial osteoblasts ( n = 4) of P2Y2R-Tg rats relative to Wt ( n = 3 (brain and lung); n = 6 (calvarial osteoblasts)). b P2Y2R protein expression in calvarial osteoblasts from WT ( n = 4) and P2Y2R-Tg ( n = 4) rats as measured by Western blotting. All bands are normalized to histone H3 expression, and mean expression in Wt cells is set at 100%. Lower panel shows membrane images of each individual experiment. Error bars represent SEM. TG P2Y2R transgenic, Wt wild-type

    Article Snippet: They were incubated overnight using rabbit anti-P2Y2 (APR-010, Alomone Labs, 1:1000) or monoclonal rabbit anti-histone H3 (#4499S, Cell Signaling, 1:1000) in blocking buffer at 4 °C on a rotor; briefly washed twice in TBS-T and then incubated with HRP-conjugated donkey anti-rabbit IgG antibody (#NA934V, GE Healthcare, 1:40.000 in blocking buffer) at RT on a rotor; washed in large volumes of TBS-T; and finally developed using ECL Select Western Blotting Detection Kit (GE Healthcare) for 5 min at RT.

    Techniques: Expressing, Western Blot, Transgenic Assay