Fluorophore Conjugated Antibodies P2x7r Atto 633, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorophore conjugated antibodies p2x7r atto 633/product/Alomone Labs
Average 90 stars, based on 3 article reviews
Price from $9.99 to $1999.99
1) Product Images from "Microglial pannexin-1 channel activation is a spinal determinant of joint pain"
Article Title: Microglial pannexin-1 channel activation is a spinal determinant of joint pain
Journal: Science Advances
Figure Legend Snippet: Spinal microglia critically contribute to MIA-induced joint pain. ( A ) Representative images (scale bar, 100 μm) and ( B ) quantification of CD11b immunofluorescence in the L3-L5 spinal dorsal horn from MIA (2 mg) and saline CTR rats 7 days after injection (CTR/Ipsi, n = 27; MIA/Ipsi, n = 21; MIA/Contra, n = 21). ( C ) Schematic depicting drug administration paradigm in rats injected with intra-articular (i.a.) MIA (2 mg) or saline (CTR) and intrathecal (i.t.) Mac1-saporin (Sap; 15 μg) or saporin (15 μg). ( D ) Mechanical paw withdrawal threshold (PWT) (CTR/Mac1-Sap, n = 4; MIA/Mac1-Sap, n = 6; MIA/Sap, n = 4). ( E ) ATP levels in rat CSF and ( F and G ) flow cytometric analysis of P2X7R expression in spinal cord cell populations 7 days after injection of MIA ( n = 9) or saline (CTR, n = 9). (F) Representative dot plot from CTR and MIA rats depicting gating parameters for CD11b − (black) and Cd11b + (blue) populations. (G) Histogram of P2X7R mean fluorescence intensity of CD11b − and CD11b + populations (MIA, n = 3; CTR, n = 3). NS, not significant. Effect of intrathecal A740003 on mechanical threshold following ( H ) continuous delivery [CTR/A740003, n = 4; MIA/A740003 10 μM, n = 5; MIA/saline (SA), n = 5] and ( I ) single injection intrathecally on day 7 (arrow) (MIA/A740003 30 μM, n = 5; MIA/SA, n = 6). * P
Techniques Used: Immunofluorescence, Injection, Expressing, Fluorescence
2) Product Images from "Site-Specific Regulation of P2X7 Receptor Function in Microglia Gates Morphine Analgesic Tolerance"
Article Title: Site-Specific Regulation of P2X7 Receptor Function in Microglia Gates Morphine Analgesic Tolerance
Journal: The Journal of Neuroscience
Figure Legend Snippet: Spinal P2X7Rs are critically involved in the development of morphine tolerance. A – D , Effects of intrathecal injections of A740003 (0.1 nmol), a selective P2X7R antagonist, on the development of morphine tolerance. A , Thermal and ( B ) mechanical nociceptive threshold in CTR- ( n = 7), MS- ( n = 7), MS/A740003- ( n = 7), and A740003- ( n = 6) treated rats. Latency to withdraw from stimulus, tail-flick latency (TFL) and paw withdrawal thresholds (PWTs), are reported as a percentage of the maximum possible effect (MPE). **** p
Techniques Used: Tail Flick Test