fluorophore conjugated antibodies p2x7r atto 633  (Alomone Labs)


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    Alomone Labs fluorophore conjugated antibodies p2x7r atto 633
    Spinal microglia critically contribute to MIA-induced joint pain. ( A ) Representative images (scale bar, 100 μm) and ( B ) quantification of CD11b immunofluorescence in the L3-L5 spinal dorsal horn from MIA (2 mg) and saline CTR rats 7 days after injection (CTR/Ipsi, n = 27; MIA/Ipsi, n = 21; MIA/Contra, n = 21). ( C ) Schematic depicting drug administration paradigm in rats injected with intra-articular (i.a.) MIA (2 mg) or saline (CTR) and intrathecal (i.t.) Mac1-saporin (Sap; 15 μg) or saporin (15 μg). ( D ) Mechanical paw withdrawal threshold (PWT) (CTR/Mac1-Sap, n = 4; MIA/Mac1-Sap, n = 6; MIA/Sap, n = 4). ( E ) ATP levels in rat CSF and ( F and G ) flow cytometric analysis of <t>P2X7R</t> expression in spinal cord cell populations 7 days after injection of MIA ( n = 9) or saline (CTR, n = 9). (F) Representative dot plot from CTR and MIA rats depicting gating parameters for CD11b − (black) and Cd11b + (blue) populations. (G) Histogram of P2X7R mean fluorescence intensity of CD11b − and CD11b + populations (MIA, n = 3; CTR, n = 3). NS, not significant. Effect of intrathecal A740003 on mechanical threshold following ( H ) continuous delivery [CTR/A740003, n = 4; MIA/A740003 10 μM, n = 5; MIA/saline (SA), n = 5] and ( I ) single injection intrathecally on day 7 (arrow) (MIA/A740003 30 μM, n = 5; MIA/SA, n = 6). * P
    Fluorophore Conjugated Antibodies P2x7r Atto 633, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorophore conjugated antibodies p2x7r atto 633/product/Alomone Labs
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    fluorophore conjugated antibodies p2x7r atto 633 - by Bioz Stars, 2022-08
    90/100 stars

    Images

    1) Product Images from "Microglial pannexin-1 channel activation is a spinal determinant of joint pain"

    Article Title: Microglial pannexin-1 channel activation is a spinal determinant of joint pain

    Journal: Science Advances

    doi: 10.1126/sciadv.aas9846

    Spinal microglia critically contribute to MIA-induced joint pain. ( A ) Representative images (scale bar, 100 μm) and ( B ) quantification of CD11b immunofluorescence in the L3-L5 spinal dorsal horn from MIA (2 mg) and saline CTR rats 7 days after injection (CTR/Ipsi, n = 27; MIA/Ipsi, n = 21; MIA/Contra, n = 21). ( C ) Schematic depicting drug administration paradigm in rats injected with intra-articular (i.a.) MIA (2 mg) or saline (CTR) and intrathecal (i.t.) Mac1-saporin (Sap; 15 μg) or saporin (15 μg). ( D ) Mechanical paw withdrawal threshold (PWT) (CTR/Mac1-Sap, n = 4; MIA/Mac1-Sap, n = 6; MIA/Sap, n = 4). ( E ) ATP levels in rat CSF and ( F and G ) flow cytometric analysis of P2X7R expression in spinal cord cell populations 7 days after injection of MIA ( n = 9) or saline (CTR, n = 9). (F) Representative dot plot from CTR and MIA rats depicting gating parameters for CD11b − (black) and Cd11b + (blue) populations. (G) Histogram of P2X7R mean fluorescence intensity of CD11b − and CD11b + populations (MIA, n = 3; CTR, n = 3). NS, not significant. Effect of intrathecal A740003 on mechanical threshold following ( H ) continuous delivery [CTR/A740003, n = 4; MIA/A740003 10 μM, n = 5; MIA/saline (SA), n = 5] and ( I ) single injection intrathecally on day 7 (arrow) (MIA/A740003 30 μM, n = 5; MIA/SA, n = 6). * P
    Figure Legend Snippet: Spinal microglia critically contribute to MIA-induced joint pain. ( A ) Representative images (scale bar, 100 μm) and ( B ) quantification of CD11b immunofluorescence in the L3-L5 spinal dorsal horn from MIA (2 mg) and saline CTR rats 7 days after injection (CTR/Ipsi, n = 27; MIA/Ipsi, n = 21; MIA/Contra, n = 21). ( C ) Schematic depicting drug administration paradigm in rats injected with intra-articular (i.a.) MIA (2 mg) or saline (CTR) and intrathecal (i.t.) Mac1-saporin (Sap; 15 μg) or saporin (15 μg). ( D ) Mechanical paw withdrawal threshold (PWT) (CTR/Mac1-Sap, n = 4; MIA/Mac1-Sap, n = 6; MIA/Sap, n = 4). ( E ) ATP levels in rat CSF and ( F and G ) flow cytometric analysis of P2X7R expression in spinal cord cell populations 7 days after injection of MIA ( n = 9) or saline (CTR, n = 9). (F) Representative dot plot from CTR and MIA rats depicting gating parameters for CD11b − (black) and Cd11b + (blue) populations. (G) Histogram of P2X7R mean fluorescence intensity of CD11b − and CD11b + populations (MIA, n = 3; CTR, n = 3). NS, not significant. Effect of intrathecal A740003 on mechanical threshold following ( H ) continuous delivery [CTR/A740003, n = 4; MIA/A740003 10 μM, n = 5; MIA/saline (SA), n = 5] and ( I ) single injection intrathecally on day 7 (arrow) (MIA/A740003 30 μM, n = 5; MIA/SA, n = 6). * P

    Techniques Used: Immunofluorescence, Injection, Expressing, Fluorescence

    2) Product Images from "Site-Specific Regulation of P2X7 Receptor Function in Microglia Gates Morphine Analgesic Tolerance"

    Article Title: Site-Specific Regulation of P2X7 Receptor Function in Microglia Gates Morphine Analgesic Tolerance

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.0852-17.2017

    Spinal P2X7Rs are critically involved in the development of morphine tolerance. A – D , Effects of intrathecal injections of A740003 (0.1 nmol), a selective P2X7R antagonist, on the development of morphine tolerance. A , Thermal and ( B ) mechanical nociceptive threshold in CTR- ( n = 7), MS- ( n = 7), MS/A740003- ( n = 7), and A740003- ( n = 6) treated rats. Latency to withdraw from stimulus, tail-flick latency (TFL) and paw withdrawal thresholds (PWTs), are reported as a percentage of the maximum possible effect (MPE). **** p
    Figure Legend Snippet: Spinal P2X7Rs are critically involved in the development of morphine tolerance. A – D , Effects of intrathecal injections of A740003 (0.1 nmol), a selective P2X7R antagonist, on the development of morphine tolerance. A , Thermal and ( B ) mechanical nociceptive threshold in CTR- ( n = 7), MS- ( n = 7), MS/A740003- ( n = 7), and A740003- ( n = 6) treated rats. Latency to withdraw from stimulus, tail-flick latency (TFL) and paw withdrawal thresholds (PWTs), are reported as a percentage of the maximum possible effect (MPE). **** p

    Techniques Used: Tail Flick Test

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    Alomone Labs fluorophore conjugated antibodies p2x7r atto 633
    Spinal microglia critically contribute to MIA-induced joint pain. ( A ) Representative images (scale bar, 100 μm) and ( B ) quantification of CD11b immunofluorescence in the L3-L5 spinal dorsal horn from MIA (2 mg) and saline CTR rats 7 days after injection (CTR/Ipsi, n = 27; MIA/Ipsi, n = 21; MIA/Contra, n = 21). ( C ) Schematic depicting drug administration paradigm in rats injected with intra-articular (i.a.) MIA (2 mg) or saline (CTR) and intrathecal (i.t.) Mac1-saporin (Sap; 15 μg) or saporin (15 μg). ( D ) Mechanical paw withdrawal threshold (PWT) (CTR/Mac1-Sap, n = 4; MIA/Mac1-Sap, n = 6; MIA/Sap, n = 4). ( E ) ATP levels in rat CSF and ( F and G ) flow cytometric analysis of <t>P2X7R</t> expression in spinal cord cell populations 7 days after injection of MIA ( n = 9) or saline (CTR, n = 9). (F) Representative dot plot from CTR and MIA rats depicting gating parameters for CD11b − (black) and Cd11b + (blue) populations. (G) Histogram of P2X7R mean fluorescence intensity of CD11b − and CD11b + populations (MIA, n = 3; CTR, n = 3). NS, not significant. Effect of intrathecal A740003 on mechanical threshold following ( H ) continuous delivery [CTR/A740003, n = 4; MIA/A740003 10 μM, n = 5; MIA/saline (SA), n = 5] and ( I ) single injection intrathecally on day 7 (arrow) (MIA/A740003 30 μM, n = 5; MIA/SA, n = 6). * P
    Fluorophore Conjugated Antibodies P2x7r Atto 633, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorophore conjugated antibodies p2x7r atto 633/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorophore conjugated antibodies p2x7r atto 633 - by Bioz Stars, 2022-08
    90/100 stars
      Buy from Supplier

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    Spinal microglia critically contribute to MIA-induced joint pain. ( A ) Representative images (scale bar, 100 μm) and ( B ) quantification of CD11b immunofluorescence in the L3-L5 spinal dorsal horn from MIA (2 mg) and saline CTR rats 7 days after injection (CTR/Ipsi, n = 27; MIA/Ipsi, n = 21; MIA/Contra, n = 21). ( C ) Schematic depicting drug administration paradigm in rats injected with intra-articular (i.a.) MIA (2 mg) or saline (CTR) and intrathecal (i.t.) Mac1-saporin (Sap; 15 μg) or saporin (15 μg). ( D ) Mechanical paw withdrawal threshold (PWT) (CTR/Mac1-Sap, n = 4; MIA/Mac1-Sap, n = 6; MIA/Sap, n = 4). ( E ) ATP levels in rat CSF and ( F and G ) flow cytometric analysis of P2X7R expression in spinal cord cell populations 7 days after injection of MIA ( n = 9) or saline (CTR, n = 9). (F) Representative dot plot from CTR and MIA rats depicting gating parameters for CD11b − (black) and Cd11b + (blue) populations. (G) Histogram of P2X7R mean fluorescence intensity of CD11b − and CD11b + populations (MIA, n = 3; CTR, n = 3). NS, not significant. Effect of intrathecal A740003 on mechanical threshold following ( H ) continuous delivery [CTR/A740003, n = 4; MIA/A740003 10 μM, n = 5; MIA/saline (SA), n = 5] and ( I ) single injection intrathecally on day 7 (arrow) (MIA/A740003 30 μM, n = 5; MIA/SA, n = 6). * P

    Journal: Science Advances

    Article Title: Microglial pannexin-1 channel activation is a spinal determinant of joint pain

    doi: 10.1126/sciadv.aas9846

    Figure Lengend Snippet: Spinal microglia critically contribute to MIA-induced joint pain. ( A ) Representative images (scale bar, 100 μm) and ( B ) quantification of CD11b immunofluorescence in the L3-L5 spinal dorsal horn from MIA (2 mg) and saline CTR rats 7 days after injection (CTR/Ipsi, n = 27; MIA/Ipsi, n = 21; MIA/Contra, n = 21). ( C ) Schematic depicting drug administration paradigm in rats injected with intra-articular (i.a.) MIA (2 mg) or saline (CTR) and intrathecal (i.t.) Mac1-saporin (Sap; 15 μg) or saporin (15 μg). ( D ) Mechanical paw withdrawal threshold (PWT) (CTR/Mac1-Sap, n = 4; MIA/Mac1-Sap, n = 6; MIA/Sap, n = 4). ( E ) ATP levels in rat CSF and ( F and G ) flow cytometric analysis of P2X7R expression in spinal cord cell populations 7 days after injection of MIA ( n = 9) or saline (CTR, n = 9). (F) Representative dot plot from CTR and MIA rats depicting gating parameters for CD11b − (black) and Cd11b + (blue) populations. (G) Histogram of P2X7R mean fluorescence intensity of CD11b − and CD11b + populations (MIA, n = 3; CTR, n = 3). NS, not significant. Effect of intrathecal A740003 on mechanical threshold following ( H ) continuous delivery [CTR/A740003, n = 4; MIA/A740003 10 μM, n = 5; MIA/saline (SA), n = 5] and ( I ) single injection intrathecally on day 7 (arrow) (MIA/A740003 30 μM, n = 5; MIA/SA, n = 6). * P

    Article Snippet: Cells were stained with fluorophore-conjugated antibodies P2X7R-ATTO 633 (1:250, Alomone) and CD11b/c-phycoerythrin (PE) (1:500, eBioscience) for 45 min at 22°C.

    Techniques: Immunofluorescence, Injection, Expressing, Fluorescence

    Spinal P2X7Rs are critically involved in the development of morphine tolerance. A – D , Effects of intrathecal injections of A740003 (0.1 nmol), a selective P2X7R antagonist, on the development of morphine tolerance. A , Thermal and ( B ) mechanical nociceptive threshold in CTR- ( n = 7), MS- ( n = 7), MS/A740003- ( n = 7), and A740003- ( n = 6) treated rats. Latency to withdraw from stimulus, tail-flick latency (TFL) and paw withdrawal thresholds (PWTs), are reported as a percentage of the maximum possible effect (MPE). **** p

    Journal: The Journal of Neuroscience

    Article Title: Site-Specific Regulation of P2X7 Receptor Function in Microglia Gates Morphine Analgesic Tolerance

    doi: 10.1523/JNEUROSCI.0852-17.2017

    Figure Lengend Snippet: Spinal P2X7Rs are critically involved in the development of morphine tolerance. A – D , Effects of intrathecal injections of A740003 (0.1 nmol), a selective P2X7R antagonist, on the development of morphine tolerance. A , Thermal and ( B ) mechanical nociceptive threshold in CTR- ( n = 7), MS- ( n = 7), MS/A740003- ( n = 7), and A740003- ( n = 6) treated rats. Latency to withdraw from stimulus, tail-flick latency (TFL) and paw withdrawal thresholds (PWTs), are reported as a percentage of the maximum possible effect (MPE). **** p

    Article Snippet: For flow cytometric analysis, cells were washed and collected in PBS containing 10% FBS, filtered through a 100 μm cell strainer, and stained with fluorophore-conjugated antibodies P2X7R-ATTO 633 (1:250; Alomone Labs) and cluster of differentiation molecule 11b (CD11b)/c-PE (1:500; eBioscience) for 45 min at 4°C with rotation.

    Techniques: Tail Flick Test