rabbit polyclonal p2x7 c terminus antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit polyclonal p2x7 c terminus antibody
    The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in  gray , P2X1 aa20–23 and equivalent residues in P2X2  underlined , and the P2X7 residues Lys 17  and Val 18  in  red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7.  C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT:  n  = 14 alone,  n  = 11 MCD,  n  = 3 cholesterol; mutants:  n  = 12 alone,  n  = 11 MCD,  n  = 3 cholesterol; *,  p
    Rabbit Polyclonal P2x7 C Terminus Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal p2x7 c terminus antibody/product/Alomone Labs
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal p2x7 c terminus antibody - by Bioz Stars, 2022-08
    86/100 stars

    Images

    1) Product Images from "Plasma Membrane Cholesterol as a Regulator of Human and Rodent P2X7 Receptor Activation and Sensitization *"

    Article Title: Plasma Membrane Cholesterol as a Regulator of Human and Rodent P2X7 Receptor Activation and Sensitization *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.574699

    The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in  gray , P2X1 aa20–23 and equivalent residues in P2X2  underlined , and the P2X7 residues Lys 17  and Val 18  in  red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7.  C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT:  n  = 14 alone,  n  = 11 MCD,  n  = 3 cholesterol; mutants:  n  = 12 alone,  n  = 11 MCD,  n  = 3 cholesterol; *,  p
    Figure Legend Snippet: The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in gray , P2X1 aa20–23 and equivalent residues in P2X2 underlined , and the P2X7 residues Lys 17 and Val 18 in red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7. C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT: n = 14 alone, n = 11 MCD, n = 3 cholesterol; mutants: n = 12 alone, n = 11 MCD, n = 3 cholesterol; *, p

    Techniques Used: Activity Assay, Sequencing, Mutagenesis, Relative Rate

    2) Product Images from "P2X7 purinoceptors contribute to the death of Schwann cells transplanted into the spinal cord"

    Article Title: P2X7 purinoceptors contribute to the death of Schwann cells transplanted into the spinal cord

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2013.343

    P2X7R-deficient SCs are resistant to ATP-induced cell death and survive better after transplantation. ( a ) Flow cytometry apoptosis assay showing that 5 mM ATP induced significant death of SCs from wild-type (WT) mice, whereas SCs from P2X7R-knockout (KO) mice did not show obvious cell death. *** P
    Figure Legend Snippet: P2X7R-deficient SCs are resistant to ATP-induced cell death and survive better after transplantation. ( a ) Flow cytometry apoptosis assay showing that 5 mM ATP induced significant death of SCs from wild-type (WT) mice, whereas SCs from P2X7R-knockout (KO) mice did not show obvious cell death. *** P

    Techniques Used: Transplantation Assay, Flow Cytometry, Apoptosis Assay, Mouse Assay, Knock-Out

    P2X7R is expressed in isolated SCs and sciatic nerves from rat and mouse. ( a ) Photomicrograph of cultured rat SCs double-immunostained for the SC marker S100 and P2X7R. ( b ) Detection of P2X7R mRNA in cultured rat SCs using PCR. ( c ) Photomicrographs of longitudinal sections through the rat sciatic nerve double-immunostained for S100 and P2X7R or NF200 and P2X7R. Scale bar, 50 μ m. ( d ) Photomicrographs of longitudinal sections through the sciatic nerves from C57Bl/6J wild-type (WT) and P2X7R-knockout (KO) mice double-immunostained for S100 and P2X7R. Scale bar, 100 μ m
    Figure Legend Snippet: P2X7R is expressed in isolated SCs and sciatic nerves from rat and mouse. ( a ) Photomicrograph of cultured rat SCs double-immunostained for the SC marker S100 and P2X7R. ( b ) Detection of P2X7R mRNA in cultured rat SCs using PCR. ( c ) Photomicrographs of longitudinal sections through the rat sciatic nerve double-immunostained for S100 and P2X7R or NF200 and P2X7R. Scale bar, 50 μ m. ( d ) Photomicrographs of longitudinal sections through the sciatic nerves from C57Bl/6J wild-type (WT) and P2X7R-knockout (KO) mice double-immunostained for S100 and P2X7R. Scale bar, 100 μ m

    Techniques Used: Isolation, Cell Culture, Marker, Polymerase Chain Reaction, Knock-Out, Mouse Assay

    Blockade of P2X7R on SCs increases their survival after transplantation. ( a ) Diagram illustrating the transplantation of GFP-expressing SCs (GFP/SCs) with or without oxATP treatment into either side of the dorsal column of rat T8 spinal cord. ( b ) Photomicrographs showing GFP/SCs transplanted into the spinal cord. Dashed line indicates midline of spinal cord. ( c ) Quantification of the areas occupied by GFP/SCs with or without oxATP pretreatment in the spinal cords of four rats (data from the same animal are linked by colored lines)
    Figure Legend Snippet: Blockade of P2X7R on SCs increases their survival after transplantation. ( a ) Diagram illustrating the transplantation of GFP-expressing SCs (GFP/SCs) with or without oxATP treatment into either side of the dorsal column of rat T8 spinal cord. ( b ) Photomicrographs showing GFP/SCs transplanted into the spinal cord. Dashed line indicates midline of spinal cord. ( c ) Quantification of the areas occupied by GFP/SCs with or without oxATP pretreatment in the spinal cords of four rats (data from the same animal are linked by colored lines)

    Techniques Used: Transplantation Assay, Expressing

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    Alomone Labs rabbit polyclonal p2x7 c terminus antibody
    The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in  gray , P2X1 aa20–23 and equivalent residues in P2X2  underlined , and the P2X7 residues Lys 17  and Val 18  in  red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7.  C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT:  n  = 14 alone,  n  = 11 MCD,  n  = 3 cholesterol; mutants:  n  = 12 alone,  n  = 11 MCD,  n  = 3 cholesterol; *,  p
    Rabbit Polyclonal P2x7 C Terminus Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal p2x7 c terminus antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal p2x7 c terminus antibody - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    93
    Alomone Labs polyclonal rabbit anti p2rx7
    BMM differentiation in the presence of POSs. A–C , Quantitative RT-PCR of Cd206 ( A ), Il-1Ra ( B ), and <t>P2rx7</t> ( C ) mRNA normalized with Rps26 mRNA of C57BL/6J- and Cx3cr1 GFP/GFP -BMMs cultured for 18 h with or without POSs ( n = 5 per group, * p
    Polyclonal Rabbit Anti P2rx7, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti p2rx7/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti p2rx7 - by Bioz Stars, 2022-08
    93/100 stars
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    93
    Alomone Labs rabbit polyclonal anti p2rx7 extracellular antibody
    <t>P2RX7</t> expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P
    Rabbit Polyclonal Anti P2rx7 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti p2rx7 extracellular antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti p2rx7 extracellular antibody - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in  gray , P2X1 aa20–23 and equivalent residues in P2X2  underlined , and the P2X7 residues Lys 17  and Val 18  in  red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7.  C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT:  n  = 14 alone,  n  = 11 MCD,  n  = 3 cholesterol; mutants:  n  = 12 alone,  n  = 11 MCD,  n  = 3 cholesterol; *,  p

    Journal: The Journal of Biological Chemistry

    Article Title: Plasma Membrane Cholesterol as a Regulator of Human and Rodent P2X7 Receptor Activation and Sensitization *

    doi: 10.1074/jbc.M114.574699

    Figure Lengend Snippet: The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in gray , P2X1 aa20–23 and equivalent residues in P2X2 underlined , and the P2X7 residues Lys 17 and Val 18 in red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7. C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT: n = 14 alone, n = 11 MCD, n = 3 cholesterol; mutants: n = 12 alone, n = 11 MCD, n = 3 cholesterol; *, p

    Article Snippet: Rabbit polyclonal P2X7 C terminus antibody was obtained from Alomone Labs (Jerusalem, Israel), and anti-mouse and anti-rabbit HRP-conjugated secondary antibodies were from Thermo Fisher Scientific and Bio-Rad, respectively.

    Techniques: Activity Assay, Sequencing, Mutagenesis, Relative Rate

    BMM differentiation in the presence of POSs. A–C , Quantitative RT-PCR of Cd206 ( A ), Il-1Ra ( B ), and P2rx7 ( C ) mRNA normalized with Rps26 mRNA of C57BL/6J- and Cx3cr1 GFP/GFP -BMMs cultured for 18 h with or without POSs ( n = 5 per group, * p

    Journal: The Journal of Neuroscience

    Article Title: Upregulation of P2RX7 in Cx3cr1-Deficient Mononuclear Phagocytes Leads to Increased Interleukin-1β Secretion and Photoreceptor Neurodegeneration

    doi: 10.1523/JNEUROSCI.3955-14.2015

    Figure Lengend Snippet: BMM differentiation in the presence of POSs. A–C , Quantitative RT-PCR of Cd206 ( A ), Il-1Ra ( B ), and P2rx7 ( C ) mRNA normalized with Rps26 mRNA of C57BL/6J- and Cx3cr1 GFP/GFP -BMMs cultured for 18 h with or without POSs ( n = 5 per group, * p

    Article Snippet: Retinal and RPE/choroid tissues were dissected intact from the globe, flat mounted, and processed for immunohistochemistry using the following primary antibody: polyclonal rabbit anti-P2RX7 (APR-008; Alomone Labs), polyclonal rabbit anti-IL-1β (ab9722; Abcam), and polyclonal goat anti-IBA1 (ab5076; Abcam).

    Techniques: Quantitative RT-PCR, Cell Culture

    Subretinal MPs in light-challenged Cx3cr1 GFP/GFP mice express IL-1β and P2RX7. A , IBA1-positive cell density in the subretinal space of C57BL/6J and Cx3cr1 GFP/GFP mice at different time points during the light-challenge model ( n = 5 per group, * p

    Journal: The Journal of Neuroscience

    Article Title: Upregulation of P2RX7 in Cx3cr1-Deficient Mononuclear Phagocytes Leads to Increased Interleukin-1β Secretion and Photoreceptor Neurodegeneration

    doi: 10.1523/JNEUROSCI.3955-14.2015

    Figure Lengend Snippet: Subretinal MPs in light-challenged Cx3cr1 GFP/GFP mice express IL-1β and P2RX7. A , IBA1-positive cell density in the subretinal space of C57BL/6J and Cx3cr1 GFP/GFP mice at different time points during the light-challenge model ( n = 5 per group, * p

    Article Snippet: Retinal and RPE/choroid tissues were dissected intact from the globe, flat mounted, and processed for immunohistochemistry using the following primary antibody: polyclonal rabbit anti-P2RX7 (APR-008; Alomone Labs), polyclonal rabbit anti-IL-1β (ab9722; Abcam), and polyclonal goat anti-IBA1 (ab5076; Abcam).

    Techniques: Mouse Assay

    Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of P2X7 mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with polyclonal C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p

    Journal: International Journal of Cancer

    Article Title: Targeting of the P2X7 receptor in pancreatic cancer and stellate cells

    doi: 10.1002/ijc.30380

    Figure Lengend Snippet: Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of P2X7 mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with polyclonal C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p

    Article Snippet: For the fluorescence detection, the slides were incubated with the relevant secondary antibody conjugated to Alexa Fluor‐568 or Alexa Fluor‐488 (Life Technologies, 1:400) for 1 hr, or directly incubated with the polyclonal antibody rabbit anti‐P2X7 receptor (extracellular)‐ATTO‐488 (Alomone APR‐008‐AG, 1:400) overnight at 4°C.

    Techniques: Expressing, Western Blot

    P2RX7 expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Nucleoside reverse transcriptase inhibitors and Kamuvudines inhibit amyloid-β induced retinal pigmented epithelium degeneration

    doi: 10.1038/s41392-021-00537-z

    Figure Lengend Snippet: P2RX7 expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P

    Article Snippet: The RPE flat mounts were stained with Dylight phalloidin 650 (1:10, Cell Signaling) and a rabbit polyclonal anti-P2RX7 (extracellular) antibody (1:100, Alomone Labs), followed by a goat anti-rabbit Alexa-555 antibody (1:200, Invitrogen).

    Techniques: Expressing, Injection, Mouse Assay, Staining, Two Tailed Test