polyclonal antibody rabbit anti p2x7 receptor  (Alomone Labs)

Journal: The Journal of Neuroscience

Article Title: Upregulation of P2RX7 in Cx3cr1-Deficient Mononuclear Phagocytes Leads to Increased Interleukin-1β Secretion and Photoreceptor Neurodegeneration

doi: 10.1523/JNEUROSCI.3955-14.2015

Figure Lengend Snippet: BMM differentiation in the presence of POSs. A–C , Quantitative RT-PCR of Cd206 ( A ), Il-1Ra ( B ), and P2rx7 ( C ) mRNA normalized with Rps26 mRNA of C57BL/6J- and Cx3cr1 GFP/GFP -BMMs cultured for 18 h with or without POSs ( n = 5 per group, * p

Article Snippet: Retinal and RPE/choroid tissues were dissected intact from the globe, flat mounted, and processed for immunohistochemistry using the following primary antibody: polyclonal rabbit anti-P2RX7 (APR-008; Alomone Labs), polyclonal rabbit anti-IL-1β (ab9722; Abcam), and polyclonal goat anti-IBA1 (ab5076; Abcam).

Techniques: Quantitative RT-PCR, Cell Culture

Journal: The Journal of Neuroscience

Article Title: Upregulation of P2RX7 in Cx3cr1-Deficient Mononuclear Phagocytes Leads to Increased Interleukin-1β Secretion and Photoreceptor Neurodegeneration

doi: 10.1523/JNEUROSCI.3955-14.2015

Figure Lengend Snippet: Subretinal MPs in light-challenged Cx3cr1 GFP/GFP mice express IL-1β and P2RX7. A , IBA1-positive cell density in the subretinal space of C57BL/6J and Cx3cr1 GFP/GFP mice at different time points during the light-challenge model ( n = 5 per group, * p

Article Snippet: Retinal and RPE/choroid tissues were dissected intact from the globe, flat mounted, and processed for immunohistochemistry using the following primary antibody: polyclonal rabbit anti-P2RX7 (APR-008; Alomone Labs), polyclonal rabbit anti-IL-1β (ab9722; Abcam), and polyclonal goat anti-IBA1 (ab5076; Abcam).

Techniques: Mouse Assay

Journal: Signal Transduction and Targeted Therapy

Article Title: Nucleoside reverse transcriptase inhibitors and Kamuvudines inhibit amyloid-β induced retinal pigmented epithelium degeneration

doi: 10.1038/s41392-021-00537-z

Figure Lengend Snippet: P2RX7 expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P

Article Snippet: The RPE flat mounts were stained with Dylight phalloidin 650 (1:10, Cell Signaling) and a rabbit polyclonal anti-P2RX7 (extracellular) antibody (1:100, Alomone Labs), followed by a goat anti-rabbit Alexa-555 antibody (1:200, Invitrogen).

Techniques: Expressing, Injection, Mouse Assay, Staining, Two Tailed Test

Journal: International Journal of Cancer

Article Title: Targeting of the P2X7 receptor in pancreatic cancer and stellate cells

doi: 10.1002/ijc.30380

Figure Lengend Snippet: Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of P2X7 mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with polyclonal C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p

Article Snippet: For the fluorescence detection, the slides were incubated with the relevant secondary antibody conjugated to Alexa Fluor‐568 or Alexa Fluor‐488 (Life Technologies, 1:400) for 1 hr, or directly incubated with the polyclonal antibody rabbit anti‐P2X7 receptor (extracellular)‐ATTO‐488 (Alomone APR‐008‐AG, 1:400) overnight at 4°C.

Techniques: Expressing, Western Blot