p2x7 anti p2x1 receptor atto 488 180  (Alomone Labs)


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    Structured Review

    Alomone Labs p2x7 anti p2x1 receptor atto 488 180
    The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in gray , P2X1 aa20–23 and equivalent residues in P2X2 underlined , and the P2X7 residues Lys 17 and Val 18 in red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7. C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT: n = 14 alone, n = 11 MCD, n = 3 cholesterol; mutants: n = 12 alone, n = 11 MCD, n = 3 cholesterol; *, p
    P2x7 Anti P2x1 Receptor Atto 488 180, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x7 anti p2x1 receptor atto 488 180/product/Alomone Labs
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    p2x7 anti p2x1 receptor atto 488 180 - by Bioz Stars, 2022-12
    88/100 stars

    Images

    1) Product Images from "Plasma Membrane Cholesterol as a Regulator of Human and Rodent P2X7 Receptor Activation and Sensitization *"

    Article Title: Plasma Membrane Cholesterol as a Regulator of Human and Rodent P2X7 Receptor Activation and Sensitization *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.574699

    The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in gray , P2X1 aa20–23 and equivalent residues in P2X2 underlined , and the P2X7 residues Lys 17 and Val 18 in red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7. C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT: n = 14 alone, n = 11 MCD, n = 3 cholesterol; mutants: n = 12 alone, n = 11 MCD, n = 3 cholesterol; *, p
    Figure Legend Snippet: The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in gray , P2X1 aa20–23 and equivalent residues in P2X2 underlined , and the P2X7 residues Lys 17 and Val 18 in red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7. C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT: n = 14 alone, n = 11 MCD, n = 3 cholesterol; mutants: n = 12 alone, n = 11 MCD, n = 3 cholesterol; *, p

    Techniques Used: Activity Assay, Sequencing, Mutagenesis, Relative Rate

    2) Product Images from "P2X7 purinoceptors contribute to the death of Schwann cells transplanted into the spinal cord"

    Article Title: P2X7 purinoceptors contribute to the death of Schwann cells transplanted into the spinal cord

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2013.343

    P2X7R-deficient SCs are resistant to ATP-induced cell death and survive better after transplantation. ( a ) Flow cytometry apoptosis assay showing that 5 mM ATP induced significant death of SCs from wild-type (WT) mice, whereas SCs from P2X7R-knockout (KO) mice did not show obvious cell death. *** P
    Figure Legend Snippet: P2X7R-deficient SCs are resistant to ATP-induced cell death and survive better after transplantation. ( a ) Flow cytometry apoptosis assay showing that 5 mM ATP induced significant death of SCs from wild-type (WT) mice, whereas SCs from P2X7R-knockout (KO) mice did not show obvious cell death. *** P

    Techniques Used: Transplantation Assay, Flow Cytometry, Apoptosis Assay, Mouse Assay, Knock-Out

    P2X7R is expressed in isolated SCs and sciatic nerves from rat and mouse. ( a ) Photomicrograph of cultured rat SCs double-immunostained for the SC marker S100 and P2X7R. ( b ) Detection of P2X7R mRNA in cultured rat SCs using PCR. ( c ) Photomicrographs of longitudinal sections through the rat sciatic nerve double-immunostained for S100 and P2X7R or NF200 and P2X7R. Scale bar, 50 μ m. ( d ) Photomicrographs of longitudinal sections through the sciatic nerves from C57Bl/6J wild-type (WT) and P2X7R-knockout (KO) mice double-immunostained for S100 and P2X7R. Scale bar, 100 μ m
    Figure Legend Snippet: P2X7R is expressed in isolated SCs and sciatic nerves from rat and mouse. ( a ) Photomicrograph of cultured rat SCs double-immunostained for the SC marker S100 and P2X7R. ( b ) Detection of P2X7R mRNA in cultured rat SCs using PCR. ( c ) Photomicrographs of longitudinal sections through the rat sciatic nerve double-immunostained for S100 and P2X7R or NF200 and P2X7R. Scale bar, 50 μ m. ( d ) Photomicrographs of longitudinal sections through the sciatic nerves from C57Bl/6J wild-type (WT) and P2X7R-knockout (KO) mice double-immunostained for S100 and P2X7R. Scale bar, 100 μ m

    Techniques Used: Isolation, Cell Culture, Marker, Polymerase Chain Reaction, Knock-Out, Mouse Assay

    Blockade of P2X7R on SCs increases their survival after transplantation. ( a ) Diagram illustrating the transplantation of GFP-expressing SCs (GFP/SCs) with or without oxATP treatment into either side of the dorsal column of rat T8 spinal cord. ( b ) Photomicrographs showing GFP/SCs transplanted into the spinal cord. Dashed line indicates midline of spinal cord. ( c ) Quantification of the areas occupied by GFP/SCs with or without oxATP pretreatment in the spinal cords of four rats (data from the same animal are linked by colored lines)
    Figure Legend Snippet: Blockade of P2X7R on SCs increases their survival after transplantation. ( a ) Diagram illustrating the transplantation of GFP-expressing SCs (GFP/SCs) with or without oxATP treatment into either side of the dorsal column of rat T8 spinal cord. ( b ) Photomicrographs showing GFP/SCs transplanted into the spinal cord. Dashed line indicates midline of spinal cord. ( c ) Quantification of the areas occupied by GFP/SCs with or without oxATP pretreatment in the spinal cords of four rats (data from the same animal are linked by colored lines)

    Techniques Used: Transplantation Assay, Expressing

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    • rabbit polyclonal anti p2rx7 extracellular antibody  (Alomone Labs)
    93
    Alomone Labs rabbit polyclonal anti p2rx7 extracellular antibody
    <t>P2RX7</t> expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P
    Rabbit Polyclonal Anti P2rx7 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti p2rx7 extracellular antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti p2rx7 extracellular antibody - by Bioz Stars, 2022-12
    93/100 stars
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    p2x7 anti p2x1 receptor atto 488 180  (Alomone Labs)
    88
    Alomone Labs p2x7 anti p2x1 receptor atto 488 180
    <t>P2RX7</t> expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P
    P2x7 Anti P2x1 Receptor Atto 488 180, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x7 anti p2x1 receptor atto 488 180/product/Alomone Labs
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    p2x7 anti p2x1 receptor atto 488 180 - by Bioz Stars, 2022-12
    88/100 stars
    		  Buy from Supplier
    polyclonal antibody rabbit anti p2x7 receptor  (Alomone Labs)
    94
    Alomone Labs polyclonal antibody rabbit anti p2x7 receptor
    Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of <t>P2X7</t> mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with <t>polyclonal</t> C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p
    Polyclonal Antibody Rabbit Anti P2x7 Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody rabbit anti p2x7 receptor/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody rabbit anti p2x7 receptor - by Bioz Stars, 2022-12
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    P2RX7 expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Nucleoside reverse transcriptase inhibitors and Kamuvudines inhibit amyloid-β induced retinal pigmented epithelium degeneration

    doi: 10.1038/s41392-021-00537-z

    Figure Lengend Snippet: P2RX7 expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P

    Article Snippet: The RPE flat mounts were stained with Dylight phalloidin 650 (1:10, Cell Signaling) and a rabbit polyclonal anti-P2RX7 (extracellular) antibody (1:100, Alomone Labs), followed by a goat anti-rabbit Alexa-555 antibody (1:200, Invitrogen).

    Techniques: Expressing, Injection, Mouse Assay, Staining, Two Tailed Test

    Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of P2X7 mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with polyclonal C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p

    Journal: International Journal of Cancer

    Article Title: Targeting of the P2X7 receptor in pancreatic cancer and stellate cells

    doi: 10.1002/ijc.30380

    Figure Lengend Snippet: Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of P2X7 mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with polyclonal C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p

    Article Snippet: For the fluorescence detection, the slides were incubated with the relevant secondary antibody conjugated to Alexa Fluor‐568 or Alexa Fluor‐488 (Life Technologies, 1:400) for 1 hr, or directly incubated with the polyclonal antibody rabbit anti‐P2X7 receptor (extracellular)‐ATTO‐488 (Alomone APR‐008‐AG, 1:400) overnight at 4°C.

    Techniques: Expressing, Western Blot