rabbit polyclonal p2x7 c terminus antibody (Alomone Labs)


Structured Review

Rabbit Polyclonal P2x7 C Terminus Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal p2x7 c terminus antibody/product/Alomone Labs
Average 86 stars, based on 3 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Plasma Membrane Cholesterol as a Regulator of Human and Rodent P2X7 Receptor Activation and Sensitization *"
Article Title: Plasma Membrane Cholesterol as a Regulator of Human and Rodent P2X7 Receptor Activation and Sensitization *
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.M114.574699

Figure Legend Snippet: The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in gray , P2X1 aa20–23 and equivalent residues in P2X2 underlined , and the P2X7 residues Lys 17 and Val 18 in red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7. C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT: n = 14 alone, n = 11 MCD, n = 3 cholesterol; mutants: n = 12 alone, n = 11 MCD, n = 3 cholesterol; *, p
Techniques Used: Activity Assay, Sequencing, Mutagenesis, Relative Rate
2) Product Images from "P2X7 purinoceptors contribute to the death of Schwann cells transplanted into the spinal cord"
Article Title: P2X7 purinoceptors contribute to the death of Schwann cells transplanted into the spinal cord
Journal: Cell Death & Disease
doi: 10.1038/cddis.2013.343

Figure Legend Snippet: P2X7R-deficient SCs are resistant to ATP-induced cell death and survive better after transplantation. ( a ) Flow cytometry apoptosis assay showing that 5 mM ATP induced significant death of SCs from wild-type (WT) mice, whereas SCs from P2X7R-knockout (KO) mice did not show obvious cell death. *** P
Techniques Used: Transplantation Assay, Flow Cytometry, Apoptosis Assay, Mouse Assay, Knock-Out

Figure Legend Snippet: P2X7R is expressed in isolated SCs and sciatic nerves from rat and mouse. ( a ) Photomicrograph of cultured rat SCs double-immunostained for the SC marker S100 and P2X7R. ( b ) Detection of P2X7R mRNA in cultured rat SCs using PCR. ( c ) Photomicrographs of longitudinal sections through the rat sciatic nerve double-immunostained for S100 and P2X7R or NF200 and P2X7R. Scale bar, 50 μ m. ( d ) Photomicrographs of longitudinal sections through the sciatic nerves from C57Bl/6J wild-type (WT) and P2X7R-knockout (KO) mice double-immunostained for S100 and P2X7R. Scale bar, 100 μ m
Techniques Used: Isolation, Cell Culture, Marker, Polymerase Chain Reaction, Knock-Out, Mouse Assay

Figure Legend Snippet: Blockade of P2X7R on SCs increases their survival after transplantation. ( a ) Diagram illustrating the transplantation of GFP-expressing SCs (GFP/SCs) with or without oxATP treatment into either side of the dorsal column of rat T8 spinal cord. ( b ) Photomicrographs showing GFP/SCs transplanted into the spinal cord. Dashed line indicates midline of spinal cord. ( c ) Quantification of the areas occupied by GFP/SCs with or without oxATP pretreatment in the spinal cords of four rats (data from the same animal are linked by colored lines)
Techniques Used: Transplantation Assay, Expressing