polyclonal antibody rabbit anti p2x7 receptor  (Alomone Labs)


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    Name:
    Anti P2X7 Receptor extracellular ATTO Fluor 488 Antibody
    Description:
    Anti P2X7 Receptor extracellular Antibody APR 008 is a highly specific antibody directed against an epitope of the mouse protein The antibody can be used in western blot indirect flow cytometry immunohistochemistry live cell imaging and immunocytochemistry applications It has been designed to recognize P2X7 purinergic receptor from mouse rat and human samples nAnti P2X7 Receptor extracellular ATTO Fluor 488 Antibody APR 008 AG is directly labeled with an ATTO 488 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 488 label is analogous to the well known dye fluorescein isothiocyanate FITC and can be used with filters typically used to detect FITC Anti P2X7 Receptor extracellular ATTO Fluor 488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers
    Catalog Number:
    APR-008-AG
    Price:
    686.0
    Category:
    Primary Antibody
    Applications:
    Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Live Cell Imaging
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal ATTO 488 (Green) Conjugated Primary Antibody
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs polyclonal antibody rabbit anti p2x7 receptor
    Anti P2X7 Receptor extracellular ATTO Fluor 488 Antibody
    Anti P2X7 Receptor extracellular Antibody APR 008 is a highly specific antibody directed against an epitope of the mouse protein The antibody can be used in western blot indirect flow cytometry immunohistochemistry live cell imaging and immunocytochemistry applications It has been designed to recognize P2X7 purinergic receptor from mouse rat and human samples nAnti P2X7 Receptor extracellular ATTO Fluor 488 Antibody APR 008 AG is directly labeled with an ATTO 488 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 488 label is analogous to the well known dye fluorescein isothiocyanate FITC and can be used with filters typically used to detect FITC Anti P2X7 Receptor extracellular ATTO Fluor 488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers
    https://www.bioz.com/result/polyclonal antibody rabbit anti p2x7 receptor/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody rabbit anti p2x7 receptor - by Bioz Stars, 2021-09
    88/100 stars

    Images

    1) Product Images from "Targeting of the P2X7 receptor in pancreatic cancer and stellate cells"

    Article Title: Targeting of the P2X7 receptor in pancreatic cancer and stellate cells

    Journal: International Journal of Cancer

    doi: 10.1002/ijc.30380

    Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of P2X7 mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with polyclonal C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p
    Figure Legend Snippet: Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of P2X7 mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with polyclonal C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p

    Techniques Used: Expressing, Western Blot

    Related Articles

    Incubation:

    Article Title: Involvement of the P2X7 Purinergic Receptor in Colonic Motor Dysfunction Associated with Bowel Inflammation in Rats
    Article Snippet: .. After Tris-buffered saline wash with 0.5% bovine serum albumin, samples were permeabilized with 0.3% Triton X-100 and incubated with rabbit anti-P2X7 receptor-ATTO-488 (1∶100; Alomone labs, Jerusalem, Israel) and with either mouse biotin-labelled anti-HuC/D (1∶100; Molecular Probes, Eugene, OR, USA) or mouse monoclonal anti-glial fibrillary acidic protein (GFAP, 1∶800; Sigma-Aldrich, Milan, Italy) for 1 hour at room temperature. ..

    Article Title: Targeting of the P2X7 receptor in pancreatic cancer and stellate cells
    Article Snippet: .. For the fluorescence detection, the slides were incubated with the relevant secondary antibody conjugated to Alexa Fluor‐568 or Alexa Fluor‐488 (Life Technologies, 1:400) for 1 hr, or directly incubated with the polyclonal antibody rabbit anti‐P2X7 receptor (extracellular)‐ATTO‐488 (Alomone APR‐008‐AG, 1:400) overnight at 4°C. ..

    Fluorescence:

    Article Title: Targeting of the P2X7 receptor in pancreatic cancer and stellate cells
    Article Snippet: .. For the fluorescence detection, the slides were incubated with the relevant secondary antibody conjugated to Alexa Fluor‐568 or Alexa Fluor‐488 (Life Technologies, 1:400) for 1 hr, or directly incubated with the polyclonal antibody rabbit anti‐P2X7 receptor (extracellular)‐ATTO‐488 (Alomone APR‐008‐AG, 1:400) overnight at 4°C. ..

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  • 95
    Alomone Labs polyclonal rabbit anti p2rx7
    BMM differentiation in the presence of POSs. A–C , Quantitative RT-PCR of Cd206 ( A ), Il-1Ra ( B ), and <t>P2rx7</t> ( C ) mRNA normalized with Rps26 mRNA of C57BL/6J- and Cx3cr1 GFP/GFP -BMMs cultured for 18 h with or without POSs ( n = 5 per group, * p
    Polyclonal Rabbit Anti P2rx7, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti p2rx7/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti p2rx7 - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    88
    Alomone Labs polyclonal antibody rabbit anti p2x7 receptor
    Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of <t>P2X7</t> mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with <t>polyclonal</t> C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p
    Polyclonal Antibody Rabbit Anti P2x7 Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody rabbit anti p2x7 receptor/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody rabbit anti p2x7 receptor - by Bioz Stars, 2021-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    BMM differentiation in the presence of POSs. A–C , Quantitative RT-PCR of Cd206 ( A ), Il-1Ra ( B ), and P2rx7 ( C ) mRNA normalized with Rps26 mRNA of C57BL/6J- and Cx3cr1 GFP/GFP -BMMs cultured for 18 h with or without POSs ( n = 5 per group, * p

    Journal: The Journal of Neuroscience

    Article Title: Upregulation of P2RX7 in Cx3cr1-Deficient Mononuclear Phagocytes Leads to Increased Interleukin-1β Secretion and Photoreceptor Neurodegeneration

    doi: 10.1523/JNEUROSCI.3955-14.2015

    Figure Lengend Snippet: BMM differentiation in the presence of POSs. A–C , Quantitative RT-PCR of Cd206 ( A ), Il-1Ra ( B ), and P2rx7 ( C ) mRNA normalized with Rps26 mRNA of C57BL/6J- and Cx3cr1 GFP/GFP -BMMs cultured for 18 h with or without POSs ( n = 5 per group, * p

    Article Snippet: Retinal and RPE/choroid tissues were dissected intact from the globe, flat mounted, and processed for immunohistochemistry using the following primary antibody: polyclonal rabbit anti-P2RX7 (APR-008; Alomone Labs), polyclonal rabbit anti-IL-1β (ab9722; Abcam), and polyclonal goat anti-IBA1 (ab5076; Abcam).

    Techniques: Quantitative RT-PCR, Cell Culture

    Subretinal MPs in light-challenged Cx3cr1 GFP/GFP mice express IL-1β and P2RX7. A , IBA1-positive cell density in the subretinal space of C57BL/6J and Cx3cr1 GFP/GFP mice at different time points during the light-challenge model ( n = 5 per group, * p

    Journal: The Journal of Neuroscience

    Article Title: Upregulation of P2RX7 in Cx3cr1-Deficient Mononuclear Phagocytes Leads to Increased Interleukin-1β Secretion and Photoreceptor Neurodegeneration

    doi: 10.1523/JNEUROSCI.3955-14.2015

    Figure Lengend Snippet: Subretinal MPs in light-challenged Cx3cr1 GFP/GFP mice express IL-1β and P2RX7. A , IBA1-positive cell density in the subretinal space of C57BL/6J and Cx3cr1 GFP/GFP mice at different time points during the light-challenge model ( n = 5 per group, * p

    Article Snippet: Retinal and RPE/choroid tissues were dissected intact from the globe, flat mounted, and processed for immunohistochemistry using the following primary antibody: polyclonal rabbit anti-P2RX7 (APR-008; Alomone Labs), polyclonal rabbit anti-IL-1β (ab9722; Abcam), and polyclonal goat anti-IBA1 (ab5076; Abcam).

    Techniques: Mouse Assay

    P2RX7 expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Nucleoside reverse transcriptase inhibitors and Kamuvudines inhibit amyloid-β induced retinal pigmented epithelium degeneration

    doi: 10.1038/s41392-021-00537-z

    Figure Lengend Snippet: P2RX7 expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P

    Article Snippet: The RPE flat mounts were stained with Dylight phalloidin 650 (1:10, Cell Signaling) and a rabbit polyclonal anti-P2RX7 (extracellular) antibody (1:100, Alomone Labs), followed by a goat anti-rabbit Alexa-555 antibody (1:200, Invitrogen).

    Techniques: Expressing, Injection, Mouse Assay, Staining, Two Tailed Test

    Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of P2X7 mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with polyclonal C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p

    Journal: International Journal of Cancer

    Article Title: Targeting of the P2X7 receptor in pancreatic cancer and stellate cells

    doi: 10.1002/ijc.30380

    Figure Lengend Snippet: Expression of P2X7R and cell proliferation with ATP, AZ10606120 and BzATP. ( a ) Representative gel of P2X7 mRNA expression (284 bp) in HPDE (H) and PancTu‐1 Luc (P) cells ( n = 3). ( b ) Western blot on the whole cell lysates with polyclonal C‐terminal antibody for P2X7R shows the A isoform (70 kDa). β actin (42 kDa) was used as loading control ( n = 4). Bar graph shows the level of P2X7R protein as a ratio to β actin. Significant difference in comparison to HPDE cells p

    Article Snippet: For the fluorescence detection, the slides were incubated with the relevant secondary antibody conjugated to Alexa Fluor‐568 or Alexa Fluor‐488 (Life Technologies, 1:400) for 1 hr, or directly incubated with the polyclonal antibody rabbit anti‐P2X7 receptor (extracellular)‐ATTO‐488 (Alomone APR‐008‐AG, 1:400) overnight at 4°C.

    Techniques: Expressing, Western Blot