p2x7r (Alomone Labs)


Structured Review

P2x7r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p2x7r/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Transient P2X7 Receptor Antagonism Produces Lasting Reductions in Spontaneous Seizures and Gliosis in Experimental Temporal Lobe Epilepsy"
Article Title: Transient P2X7 Receptor Antagonism Produces Lasting Reductions in Spontaneous Seizures and Gliosis in Experimental Temporal Lobe Epilepsy
Journal: The Journal of Neuroscience
doi: 10.1523/JNEUROSCI.4009-15.2016

Figure Legend Snippet: Increased expression of P2X7R in experimental and human epilepsy. A , Photomicrograph showing EGFP immunofluorescence in the hippocampus of a control P2rx7-EGFP mouse. Scale bar, 125 μm. Panels below show higher-power images of CA1 (i) and DG (ii) hippocampal subfields from the P2rx7-EGFP control animal. Scale bar, 50 μm. Note that the constitutive EGFP appears mainly in cells of the DG. B , Photomicrograph showing EGFP immunofluorescence in the hippocampus of an epileptic P2rx7-EGFP mouse 14 d after status epilepticus. Scale bar, 125 μm. Panels below show higher-power images of CA1 (i) and DG (ii) hippocampal subfields from the P2rx7-EGFP epileptic animal. Scale bar, 50 μm. C , Double-stained confocal immunofluorescence images showing EGFP (green, GFP) and neuronal marker NeuN (red), in the CA1 and DG subfields from epileptic P2rx7-EGFP mice. Scale bar, 50 μm. D , Immunofluorescence staining showing EGFP (green, GFP) and microglia marker Iba1 (red) in the CA3 subfield of a P2rx7-EGFP epileptic mouse. Scale bar, 20 μm. Images are representative of data from n = 5 animals per group. E , Graph showing P2rx7 mRNA levels in the CA1, CA3, and DG subfields of control (Con) and epileptic (Epi) mice ( n = 9/group; ANOVA with Bonferroni test, * p
Techniques Used: Expressing, Immunofluorescence, Staining, Marker, Mouse Assay

Figure Legend Snippet: Decreased astrogliosis and microgliosis in JNJ-47965567-treated epileptic mice. A–D , Photomicrographs of representative Iba1 staining of the hippocampus from vehicle- (Veh) and JNJ-47965567 (JNJ)-treated epileptic mice showing field views ( A ) and 20× lens magnifications ( B–D ) of individual subfields from the same animal. Note, the decrease in the number of Iba1 + cells and the immunoreactivity in mice treated with the specific P2X7R inhibitor JNJ-47965567. E , Graph showing the quantification of Iba1 + microglia found in each hippocampal subfield of vehicle- and JNJ-47965567-treated mice killed 6 d after drug washout at the end of recordings ( n = 9/group; ANOVA with Bonferroni test, ** p
Techniques Used: Mouse Assay, Staining