Journal: International Journal of Molecular Sciences
Article Title: Effects of Suramin on Polycystic Kidney Disease in a Mouse Model of Polycystin-1 Deficiency
Figure Lengend Snippet: The expression of P2X7 receptors in wild-type and Pkd1- miR Tg mice. ( A ) Representative images of kidney sections showing diffuse immunostaining for P2X7 receptors in renal tubules in wild-type mice (n = 5). ( B ) Strong staining for P2X7 receptors is noted in the cyst-lining epithelial cells in the Pkd1 miR Tg mice at 30 days of postnatal age (n = 5). ( C ) Diffuse staining for P2X7 receptors in cyst walls and non-cystic tubules in the Pkd1 -miR Tg mice at 60 days of age (n = 5). ( D ) Comparison of the renal mRNA expression of P2rx7 and other pro-inflammatory and pro-fibrotic genes in the kidneys of wild-type and Pkd1 miR Tg mice according to different postnatal ages (n = 10, 9, 5, 7, respectively). Data are mean ± SEM. Scale bar, 50 µm.
Article Snippet: Fibronectin and P2X7 receptor immunostaining were performed using a rabbit anti-fibronectin antibody (1:250, ab2413; Abcam, Cambridge, UK) and a rabbit polyclonal anti-P2X7 receptor ATTO 550 antibody (1: 50, APR 004 AO; Alomone Laboratories, Jerusalem, Israel) overnight at 4℃, respectively.
Techniques: Expressing, Mouse Assay, Immunostaining, Staining