p2rx1  (Alomone Labs)


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    Alomone Labs p2rx1
    Repression of <t>P2rx1</t> by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p
    P2rx1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2rx1/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    p2rx1 - by Bioz Stars, 2022-11
    93/100 stars

    Images

    1) Product Images from "Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels"

    Article Title: Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-52811-4

    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p
    Figure Legend Snippet: Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p

    Techniques Used: Polymerase Chain Reaction, Western Blot, Expressing, Immunohistochemistry, Staining

    2) Product Images from "Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels"

    Article Title: Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-52811-4

    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p
    Figure Legend Snippet: Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p

    Techniques Used: Polymerase Chain Reaction, Western Blot, Expressing, Immunohistochemistry, Staining

    3) Product Images from "Enhanced electrical field stimulated nitrergic and purinergic vasoreactivity in distal versus proximal internal pudendal arteries"

    Article Title: Enhanced electrical field stimulated nitrergic and purinergic vasoreactivity in distal versus proximal internal pudendal arteries

    Journal: The journal of sexual medicine

    doi: 10.1016/j.jsxm.2017.09.013

    Effectiveness of P2X1 and P2Y1 antagonists (A) NF449 (10 −5 M) eliminated α,ß-MetATP induced contractions in proximal (n=6) and (B) distal IPA (n=6). (C) MRS2500 (10 −6 M) did not impair ADP induced relaxation in proximal IPA (n=6) but (D) did decrease relaxation in distal IPA (n=6). (E) MRS2500 did not impair 2-MeSADP induced proximal IPA relaxation (n=8) or (F) reduce distal IPA relaxation to 2-MeSADP (n=8). For all values, mean ± SEM and *p
    Figure Legend Snippet: Effectiveness of P2X1 and P2Y1 antagonists (A) NF449 (10 −5 M) eliminated α,ß-MetATP induced contractions in proximal (n=6) and (B) distal IPA (n=6). (C) MRS2500 (10 −6 M) did not impair ADP induced relaxation in proximal IPA (n=6) but (D) did decrease relaxation in distal IPA (n=6). (E) MRS2500 did not impair 2-MeSADP induced proximal IPA relaxation (n=8) or (F) reduce distal IPA relaxation to 2-MeSADP (n=8). For all values, mean ± SEM and *p

    Techniques Used: Indirect Immunoperoxidase Assay

    No differences in P2X1 and P2Y1 receptor protein expression (A) P2X1 receptor expression in proximal and distal IPA normalized to ß-actin. (B) P2Y1 receptor expression in proximal and distal IPA normalized to ß-actin. For all values, n=8/group and mean ± SEM.
    Figure Legend Snippet: No differences in P2X1 and P2Y1 receptor protein expression (A) P2X1 receptor expression in proximal and distal IPA normalized to ß-actin. (B) P2Y1 receptor expression in proximal and distal IPA normalized to ß-actin. For all values, n=8/group and mean ± SEM.

    Techniques Used: Expressing, Indirect Immunoperoxidase Assay

    Purinergic antagonism improves distal IPA NANC relaxation (A) NANC relaxation with addition of selective P2X1 antagonist, NF449, in proximal and (B) distal IPA (n=8/segment). (C) NANC relaxation with addition of selective P2Y1 antagonist, MRS2500, in proximal and (D) distal IPA (n=8/segment). (E) Combined P2X1 and P2Y1 receptor antagonism in NANC relaxation using NF449 and MRS2500 in proximal and (F) distal IPA (n=8/segment). All values are represented as percent decrease from PE precontraction. For all values, mean ± SEM and *p
    Figure Legend Snippet: Purinergic antagonism improves distal IPA NANC relaxation (A) NANC relaxation with addition of selective P2X1 antagonist, NF449, in proximal and (B) distal IPA (n=8/segment). (C) NANC relaxation with addition of selective P2Y1 antagonist, MRS2500, in proximal and (D) distal IPA (n=8/segment). (E) Combined P2X1 and P2Y1 receptor antagonism in NANC relaxation using NF449 and MRS2500 in proximal and (F) distal IPA (n=8/segment). All values are represented as percent decrease from PE precontraction. For all values, mean ± SEM and *p

    Techniques Used: Indirect Immunoperoxidase Assay

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    Alomone Labs anti p2x1 receptor antibody
    EROS regulates P2X7 protein abundance by direct interaction and independently of the NADPH oxidase. (A-C) P2X7 expression analysed by western blotting of macrophages isolated from control, EROS -/- (A) and gp91 phox -/- mice (B) and of control PLB985 cells and an EROS knock-out clone (C). (D-E) P2X7 expression in RAW264.7 cells overexpressing a FLAG-tagged EROS vector (D) and in HEK293 cells transiently expressing the specified constructs (E). (F-G) Interaction between EROS and P2X7 probed by immunoprecipitation of EROS from RAW264.7 EROS-FLAG macrophages followed by immunoblot for P2X7 (F) and by NanoBIT assay in live HEK293 cells expressing the LgBIT-fused EROS vector with a SmBIT-fused P2X7 vector (G). (H) <t>P2X1</t> expression in macrophages isolated from EROS -/- mice compared to control. n= 5 biological replicates. (I) P2X1 abundance upon co-transfection with EROS construct in HEK293 cells. n= representative of 3 independent experiments. See also Figure S3.
    Anti P2x1 Receptor Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p2x1 receptor antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p2x1 receptor antibody - by Bioz Stars, 2022-11
    94/100 stars
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    EROS regulates P2X7 protein abundance by direct interaction and independently of the NADPH oxidase. (A-C) P2X7 expression analysed by western blotting of macrophages isolated from control, EROS -/- (A) and gp91 phox -/- mice (B) and of control PLB985 cells and an EROS knock-out clone (C). (D-E) P2X7 expression in RAW264.7 cells overexpressing a FLAG-tagged EROS vector (D) and in HEK293 cells transiently expressing the specified constructs (E). (F-G) Interaction between EROS and P2X7 probed by immunoprecipitation of EROS from RAW264.7 EROS-FLAG macrophages followed by immunoblot for P2X7 (F) and by NanoBIT assay in live HEK293 cells expressing the LgBIT-fused EROS vector with a SmBIT-fused P2X7 vector (G). (H) P2X1 expression in macrophages isolated from EROS -/- mice compared to control. n= 5 biological replicates. (I) P2X1 abundance upon co-transfection with EROS construct in HEK293 cells. n= representative of 3 independent experiments. See also Figure S3.

    Journal: bioRxiv

    Article Title: EROS-mediated control of NOX2 and P2X7 biosynthesis

    doi: 10.1101/2021.09.14.460103

    Figure Lengend Snippet: EROS regulates P2X7 protein abundance by direct interaction and independently of the NADPH oxidase. (A-C) P2X7 expression analysed by western blotting of macrophages isolated from control, EROS -/- (A) and gp91 phox -/- mice (B) and of control PLB985 cells and an EROS knock-out clone (C). (D-E) P2X7 expression in RAW264.7 cells overexpressing a FLAG-tagged EROS vector (D) and in HEK293 cells transiently expressing the specified constructs (E). (F-G) Interaction between EROS and P2X7 probed by immunoprecipitation of EROS from RAW264.7 EROS-FLAG macrophages followed by immunoblot for P2X7 (F) and by NanoBIT assay in live HEK293 cells expressing the LgBIT-fused EROS vector with a SmBIT-fused P2X7 vector (G). (H) P2X1 expression in macrophages isolated from EROS -/- mice compared to control. n= 5 biological replicates. (I) P2X1 abundance upon co-transfection with EROS construct in HEK293 cells. n= representative of 3 independent experiments. See also Figure S3.

    Article Snippet: Antibodies and reagents The following primary antibodies were used: mouse anti-gp91phox (sc-130543, Santa-Cruz Biotechnology; dilution 1:2000), rabbit anti-C17ORF62/EROS (HPA045696, Atlas; dilution 1:1000), rabbit anti-p22phox (sc20781, Santa-Cruz Biotechnology; dilution 1:1000), mouse anti-p22phox (sc-130550, Santa-Cruz Biotechnology; dilution 1:500), rabbit anti-P2X1 (APR001, Alomone; dilution 1:250), rabbit anti-P2X4 (APR002, Alomone; dilution 1:500), rabbit anti-P2X7 (APR004, Alomone; dilution 1:500), rabbit anti-vinculin (4650, Cell Signalling Technology; dilution 1:1000), rabbit anti-actin (ab8227, Abcam, dilution 1:2000), mouse anti-α-tubulin (ab7291, Abcam, dilution 1:1000), mouse anti-calnexin (MA3-027, Invitrogen, dilution 1:1000) and mouse anti-FLAG-M2 (F3165, Sigma, dilution 1:500).

    Techniques: Expressing, Western Blot, Isolation, Mouse Assay, Knock-Out, Plasmid Preparation, Construct, Immunoprecipitation, Cotransfection