Bkα, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Specific BK Channel Activator NS11021 Protects Rat Renal Proximal Tubular Cells from Cold Storage—Induced Mitochondrial Injury In Vitro"
Article Title: Specific BK Channel Activator NS11021 Protects Rat Renal Proximal Tubular Cells from Cold Storage—Induced Mitochondrial Injury In Vitro
Figure Legend Snippet: BK channels are detected in mitochondrial fractions from normal rat kidney proximal tubular epithelial (NRK) cells. Western blot shows expression of the pore-forming BKα subunit in mitochondrial fractions ( a ) and cytosolic fractions ( c ) from control NRK cells and after exposure to cold storage and rewarming (CS + RW). Manganese superoxide dismutase (MnSOD) served as a mitochondrial marker and loading control for mitochondrial fractions. Proteasome subunit beta type-5 (PSMB5) was used as a cytosolic marker and β-actin was used as a standard loading control. Representative blots are shown using n = 3, where each lane is loaded with 25 μg protein corresponding to a separate experiment. Densitometry analyses for the mitochondrial ( b ) and cytosolic ( d ) fractions are next to corresponding blots; densitometry calculated from two separate blots with a total n = 6; no significant differences detected using p
Techniques Used: Western Blot, Expressing, Marker