kca2 3 antibodies  (Alomone Labs)


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    Alomone Labs kca2 3 antibodies
    Expression of <t>SK1–SK3</t> channels in the hypothalamic PVN. A : Western blot analysis showing protein expression of SK1–SK3 channels between rats in the NS control and ANG II-salt HTN groups. B : summary data showing no significant statistical difference in protein expression of SK1–SK3 channels in the punched PVN between the NS control group ( n = 7) and ANG II-salt HTN group ( n = 7). All values were normalized to β-actin.
    Kca2 3 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kca2 3 antibodies/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kca2 3 antibodies - by Bioz Stars, 2022-05
    92/100 stars

    Images

    1) Product Images from "Sympathoexcitation in ANG II-salt hypertension involves reduced SK channel function in the hypothalamic paraventricular nucleus"

    Article Title: Sympathoexcitation in ANG II-salt hypertension involves reduced SK channel function in the hypothalamic paraventricular nucleus

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00832.2014

    Expression of SK1–SK3 channels in the hypothalamic PVN. A : Western blot analysis showing protein expression of SK1–SK3 channels between rats in the NS control and ANG II-salt HTN groups. B : summary data showing no significant statistical difference in protein expression of SK1–SK3 channels in the punched PVN between the NS control group ( n = 7) and ANG II-salt HTN group ( n = 7). All values were normalized to β-actin.
    Figure Legend Snippet: Expression of SK1–SK3 channels in the hypothalamic PVN. A : Western blot analysis showing protein expression of SK1–SK3 channels between rats in the NS control and ANG II-salt HTN groups. B : summary data showing no significant statistical difference in protein expression of SK1–SK3 channels in the punched PVN between the NS control group ( n = 7) and ANG II-salt HTN group ( n = 7). All values were normalized to β-actin.

    Techniques Used: Expressing, Western Blot

    2) Product Images from "Mineralocorticoid receptor is involved in rat and human ocular chorioretinopathy"

    Article Title: Mineralocorticoid receptor is involved in rat and human ocular chorioretinopathy

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI61427

    Expression and aldosterone regulation of KCa2.3 in rat eyes.
    Figure Legend Snippet: Expression and aldosterone regulation of KCa2.3 in rat eyes.

    Techniques Used: Expressing

    Aldosterone-induced choroidal thickening depends on KCa2.3 activity in rat eyes.
    Figure Legend Snippet: Aldosterone-induced choroidal thickening depends on KCa2.3 activity in rat eyes.

    Techniques Used: Activity Assay

    3) Product Images from "Immunolocalization and expression of small-conductance calcium-activated potassium channels in human myometrium"

    Article Title: Immunolocalization and expression of small-conductance calcium-activated potassium channels in human myometrium

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/j.1582-4934.2012.01627.x

    Representative sections of human pregnant myometrial preparation. Weak staining for SK3 (A), but strong positive staining for CD34 (B) corresponding to telocytes intermingling muscle bundles (black arrows). Pregnant myometrium showed loss of SK3 in telocytes. SK3 and CD34 were also detected in vascular endothelium (red arrows). Only normal mast cells stained for CD117 (C; magnification × 600). Section overview labelling for smooth muscles actin (D) Magnification × 200 inset ×600.
    Figure Legend Snippet: Representative sections of human pregnant myometrial preparation. Weak staining for SK3 (A), but strong positive staining for CD34 (B) corresponding to telocytes intermingling muscle bundles (black arrows). Pregnant myometrium showed loss of SK3 in telocytes. SK3 and CD34 were also detected in vascular endothelium (red arrows). Only normal mast cells stained for CD117 (C; magnification × 600). Section overview labelling for smooth muscles actin (D) Magnification × 200 inset ×600.

    Techniques Used: Staining

    Representative immunostaining of formalin-fixed paraffin-embedded human non-pregnant myometrial section (4 μm). Strong positive staining for SK3 (A) and CD34 (B) corresponding to telocytes intermingling muscle bundles (black arrows). SK3 and CD34 were also detected in vascular endothelium (red arrows). Only normal mast cells stained for CD117 (C; magnification × 600) and section overview labelling for smooth muscles actin (D) Magnification × 200 inserts × 600.
    Figure Legend Snippet: Representative immunostaining of formalin-fixed paraffin-embedded human non-pregnant myometrial section (4 μm). Strong positive staining for SK3 (A) and CD34 (B) corresponding to telocytes intermingling muscle bundles (black arrows). SK3 and CD34 were also detected in vascular endothelium (red arrows). Only normal mast cells stained for CD117 (C; magnification × 600) and section overview labelling for smooth muscles actin (D) Magnification × 200 inserts × 600.

    Techniques Used: Immunostaining, Formalin-fixed Paraffin-Embedded, Staining

    Double immune-fluorescence labelling for SK3 (red) and smooth muscles actin or CD34 (green) in human non-pregnant (A and B) and pregnant (C and D) myometrium and appendiceal muscularis propria (E and F). interstitial Cajal-like cells (ICLC) displayed co-expression for SK3 and CD34 (yellow reaction product) in human non-pregnant myometrium (B) and appendiceal muscularis propria (F) (arrows), whereas pregnant myometrium lacked SK3 in ICLC and only vascular endothelium displayed co-expression for both SK3 and CD34 (arrow-head). DAPI nuclear labelling in blue. Also, note the lower cellular density in pregnant myometrium. Magnification × 600.
    Figure Legend Snippet: Double immune-fluorescence labelling for SK3 (red) and smooth muscles actin or CD34 (green) in human non-pregnant (A and B) and pregnant (C and D) myometrium and appendiceal muscularis propria (E and F). interstitial Cajal-like cells (ICLC) displayed co-expression for SK3 and CD34 (yellow reaction product) in human non-pregnant myometrium (B) and appendiceal muscularis propria (F) (arrows), whereas pregnant myometrium lacked SK3 in ICLC and only vascular endothelium displayed co-expression for both SK3 and CD34 (arrow-head). DAPI nuclear labelling in blue. Also, note the lower cellular density in pregnant myometrium. Magnification × 600.

    Techniques Used: Fluorescence, Expressing

    KCNN3 expression measured by qRT-PCR. The relative expression of KCNN3 is normalized to the geometric mean of the most stable reference genes, SDHA and TOP1. Bar chart showing expression of KCNN3 in non-pregnant (blue, n = 11) and pregnant (red, n = 11) (A) Bar chart showing KCNN3 expression in each patient (B) Values are presented as mean ± S.E.M. * P > 0.05.
    Figure Legend Snippet: KCNN3 expression measured by qRT-PCR. The relative expression of KCNN3 is normalized to the geometric mean of the most stable reference genes, SDHA and TOP1. Bar chart showing expression of KCNN3 in non-pregnant (blue, n = 11) and pregnant (red, n = 11) (A) Bar chart showing KCNN3 expression in each patient (B) Values are presented as mean ± S.E.M. * P > 0.05.

    Techniques Used: Expressing, Quantitative RT-PCR

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    Alomone Labs kca2 3 antibodies
    Expression of <t>SK1–SK3</t> channels in the hypothalamic PVN. A : Western blot analysis showing protein expression of SK1–SK3 channels between rats in the NS control and ANG II-salt HTN groups. B : summary data showing no significant statistical difference in protein expression of SK1–SK3 channels in the punched PVN between the NS control group ( n = 7) and ANG II-salt HTN group ( n = 7). All values were normalized to β-actin.
    Kca2 3 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kca2 3 antibodies/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kca2 3 antibodies - by Bioz Stars, 2022-05
    92/100 stars
      Buy from Supplier

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    Expression of SK1–SK3 channels in the hypothalamic PVN. A : Western blot analysis showing protein expression of SK1–SK3 channels between rats in the NS control and ANG II-salt HTN groups. B : summary data showing no significant statistical difference in protein expression of SK1–SK3 channels in the punched PVN between the NS control group ( n = 7) and ANG II-salt HTN group ( n = 7). All values were normalized to β-actin.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Sympathoexcitation in ANG II-salt hypertension involves reduced SK channel function in the hypothalamic paraventricular nucleus

    doi: 10.1152/ajpheart.00832.2014

    Figure Lengend Snippet: Expression of SK1–SK3 channels in the hypothalamic PVN. A : Western blot analysis showing protein expression of SK1–SK3 channels between rats in the NS control and ANG II-salt HTN groups. B : summary data showing no significant statistical difference in protein expression of SK1–SK3 channels in the punched PVN between the NS control group ( n = 7) and ANG II-salt HTN group ( n = 7). All values were normalized to β-actin.

    Article Snippet: Membranes were blocked with 5% nonfat milk for 1 h at room temperature, washed three times, and probed with primary rabbit KCa2.1, KCa2.2, and KCa2.3 antibodies (Alomone, 1:500) or mouse anti-β-actin (Santa Cruz Biotechnology) overnight at 4°C.

    Techniques: Expressing, Western Blot

    Expression and aldosterone regulation of KCa2.3 in rat eyes.

    Journal: The Journal of Clinical Investigation

    Article Title: Mineralocorticoid receptor is involved in rat and human ocular chorioretinopathy

    doi: 10.1172/JCI61427

    Figure Lengend Snippet: Expression and aldosterone regulation of KCa2.3 in rat eyes.

    Article Snippet: The following primary antibodies were used on cryosections: rabbit anti-KCa2.3 (1:100; Alomone Labs) associated with mouse anti-SMA (1:500; Abcam) or monoclonal antibody against rat CD31 (PECAM-1, 1:50; BD Biosciences — Pharmingen) to identify endothelial cells.

    Techniques: Expressing

    Aldosterone-induced choroidal thickening depends on KCa2.3 activity in rat eyes.

    Journal: The Journal of Clinical Investigation

    Article Title: Mineralocorticoid receptor is involved in rat and human ocular chorioretinopathy

    doi: 10.1172/JCI61427

    Figure Lengend Snippet: Aldosterone-induced choroidal thickening depends on KCa2.3 activity in rat eyes.

    Article Snippet: The following primary antibodies were used on cryosections: rabbit anti-KCa2.3 (1:100; Alomone Labs) associated with mouse anti-SMA (1:500; Abcam) or monoclonal antibody against rat CD31 (PECAM-1, 1:50; BD Biosciences — Pharmingen) to identify endothelial cells.

    Techniques: Activity Assay

    Representative sections of human pregnant myometrial preparation. Weak staining for SK3 (A), but strong positive staining for CD34 (B) corresponding to telocytes intermingling muscle bundles (black arrows). Pregnant myometrium showed loss of SK3 in telocytes. SK3 and CD34 were also detected in vascular endothelium (red arrows). Only normal mast cells stained for CD117 (C; magnification × 600). Section overview labelling for smooth muscles actin (D) Magnification × 200 inset ×600.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Immunolocalization and expression of small-conductance calcium-activated potassium channels in human myometrium

    doi: 10.1111/j.1582-4934.2012.01627.x

    Figure Lengend Snippet: Representative sections of human pregnant myometrial preparation. Weak staining for SK3 (A), but strong positive staining for CD34 (B) corresponding to telocytes intermingling muscle bundles (black arrows). Pregnant myometrium showed loss of SK3 in telocytes. SK3 and CD34 were also detected in vascular endothelium (red arrows). Only normal mast cells stained for CD117 (C; magnification × 600). Section overview labelling for smooth muscles actin (D) Magnification × 200 inset ×600.

    Article Snippet: In addition, the specificity of the KCa2.3 (SK3) antibody was assessed using a specific blocking peptid (Alomone labs) in accordance with the manufacturer's instructions.

    Techniques: Staining

    Representative immunostaining of formalin-fixed paraffin-embedded human non-pregnant myometrial section (4 μm). Strong positive staining for SK3 (A) and CD34 (B) corresponding to telocytes intermingling muscle bundles (black arrows). SK3 and CD34 were also detected in vascular endothelium (red arrows). Only normal mast cells stained for CD117 (C; magnification × 600) and section overview labelling for smooth muscles actin (D) Magnification × 200 inserts × 600.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Immunolocalization and expression of small-conductance calcium-activated potassium channels in human myometrium

    doi: 10.1111/j.1582-4934.2012.01627.x

    Figure Lengend Snippet: Representative immunostaining of formalin-fixed paraffin-embedded human non-pregnant myometrial section (4 μm). Strong positive staining for SK3 (A) and CD34 (B) corresponding to telocytes intermingling muscle bundles (black arrows). SK3 and CD34 were also detected in vascular endothelium (red arrows). Only normal mast cells stained for CD117 (C; magnification × 600) and section overview labelling for smooth muscles actin (D) Magnification × 200 inserts × 600.

    Article Snippet: In addition, the specificity of the KCa2.3 (SK3) antibody was assessed using a specific blocking peptid (Alomone labs) in accordance with the manufacturer's instructions.

    Techniques: Immunostaining, Formalin-fixed Paraffin-Embedded, Staining

    Double immune-fluorescence labelling for SK3 (red) and smooth muscles actin or CD34 (green) in human non-pregnant (A and B) and pregnant (C and D) myometrium and appendiceal muscularis propria (E and F). interstitial Cajal-like cells (ICLC) displayed co-expression for SK3 and CD34 (yellow reaction product) in human non-pregnant myometrium (B) and appendiceal muscularis propria (F) (arrows), whereas pregnant myometrium lacked SK3 in ICLC and only vascular endothelium displayed co-expression for both SK3 and CD34 (arrow-head). DAPI nuclear labelling in blue. Also, note the lower cellular density in pregnant myometrium. Magnification × 600.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Immunolocalization and expression of small-conductance calcium-activated potassium channels in human myometrium

    doi: 10.1111/j.1582-4934.2012.01627.x

    Figure Lengend Snippet: Double immune-fluorescence labelling for SK3 (red) and smooth muscles actin or CD34 (green) in human non-pregnant (A and B) and pregnant (C and D) myometrium and appendiceal muscularis propria (E and F). interstitial Cajal-like cells (ICLC) displayed co-expression for SK3 and CD34 (yellow reaction product) in human non-pregnant myometrium (B) and appendiceal muscularis propria (F) (arrows), whereas pregnant myometrium lacked SK3 in ICLC and only vascular endothelium displayed co-expression for both SK3 and CD34 (arrow-head). DAPI nuclear labelling in blue. Also, note the lower cellular density in pregnant myometrium. Magnification × 600.

    Article Snippet: In addition, the specificity of the KCa2.3 (SK3) antibody was assessed using a specific blocking peptid (Alomone labs) in accordance with the manufacturer's instructions.

    Techniques: Fluorescence, Expressing

    KCNN3 expression measured by qRT-PCR. The relative expression of KCNN3 is normalized to the geometric mean of the most stable reference genes, SDHA and TOP1. Bar chart showing expression of KCNN3 in non-pregnant (blue, n = 11) and pregnant (red, n = 11) (A) Bar chart showing KCNN3 expression in each patient (B) Values are presented as mean ± S.E.M. * P > 0.05.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Immunolocalization and expression of small-conductance calcium-activated potassium channels in human myometrium

    doi: 10.1111/j.1582-4934.2012.01627.x

    Figure Lengend Snippet: KCNN3 expression measured by qRT-PCR. The relative expression of KCNN3 is normalized to the geometric mean of the most stable reference genes, SDHA and TOP1. Bar chart showing expression of KCNN3 in non-pregnant (blue, n = 11) and pregnant (red, n = 11) (A) Bar chart showing KCNN3 expression in each patient (B) Values are presented as mean ± S.E.M. * P > 0.05.

    Article Snippet: In addition, the specificity of the KCa2.3 (SK3) antibody was assessed using a specific blocking peptid (Alomone labs) in accordance with the manufacturer's instructions.

    Techniques: Expressing, Quantitative RT-PCR