apc101  (Alomone Labs)


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    Alomone Labs apc101
    Apc101, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apc101/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apc101 - by Bioz Stars, 2022-08
    94/100 stars

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    Alomone Labs anti kv1 3
    Inhibition of mitochondrial <t>Kv1.3</t> kills PANC-1 cells. (A) PANC-1 cells were treated or left untreated with different membrane permeant Kv1.3 inhibitors (PAPTP 10 µM, PCARBTP 10 µM, and clofazimine 20 µM) for 24 h. Cell death was determined by staining with an Alexa568 coupled Annexin V by fluorescent microscopy. The images are representative of three different replicates. (B) Quantification of Annexin V-positive cells from the experiments shown in (A) : represented is the percentage of Annexin V-positive cells ± SEM ( n = 3; ** p
    Anti Kv1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv1 3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv1 3 - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    86
    The Jackson Laboratory anti kv1 3 kcna3 extracellular antibody
    <t>Kv1.3</t> and KCa3.1 currents are expressed in D10 cells. ( A ): Representative Kv1.3 K + current in a single D10 cell recorded during a 1.5-s-long test pulse to +50 mV from a holding potential of –120 mV. The superimposed dashed line indicates the best fit single exponential with τ =252 ms. ( B ): Representative Kv1.3 K + currents from a single D10 cell in control solution, and after the equilibration of the block in the presence of 15 pM MgTx (test pulse: +50 mV). ( C ): Voltage ramps from –120 mV to +50 mV (duration: 150 ms) evoked KCa3.1 currents from a single D10 cell. Traces show the current in control solution, after the equilibration of the block in the presence of 250 nM TRAM-34, and after wash-out. The voltage range below the activation threshold of Kv1.3 channels is shown only.
    Anti Kv1 3 Kcna3 Extracellular Antibody, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv1 3 kcna3 extracellular antibody/product/The Jackson Laboratory
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv1 3 kcna3 extracellular antibody - by Bioz Stars, 2022-08
    86/100 stars
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    Image Search Results


    Inhibition of mitochondrial Kv1.3 kills PANC-1 cells. (A) PANC-1 cells were treated or left untreated with different membrane permeant Kv1.3 inhibitors (PAPTP 10 µM, PCARBTP 10 µM, and clofazimine 20 µM) for 24 h. Cell death was determined by staining with an Alexa568 coupled Annexin V by fluorescent microscopy. The images are representative of three different replicates. (B) Quantification of Annexin V-positive cells from the experiments shown in (A) : represented is the percentage of Annexin V-positive cells ± SEM ( n = 3; ** p

    Journal: Frontiers in Oncology

    Article Title: Regulation of Proliferation by a Mitochondrial Potassium Channel in Pancreatic Ductal Adenocarcinoma Cells

    doi: 10.3389/fonc.2017.00239

    Figure Lengend Snippet: Inhibition of mitochondrial Kv1.3 kills PANC-1 cells. (A) PANC-1 cells were treated or left untreated with different membrane permeant Kv1.3 inhibitors (PAPTP 10 µM, PCARBTP 10 µM, and clofazimine 20 µM) for 24 h. Cell death was determined by staining with an Alexa568 coupled Annexin V by fluorescent microscopy. The images are representative of three different replicates. (B) Quantification of Annexin V-positive cells from the experiments shown in (A) : represented is the percentage of Annexin V-positive cells ± SEM ( n = 3; ** p

    Article Snippet: After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-Kv1.3 (1:200, rabbit polyclonal; Alomone Labs APC-101); anti-GAPDH (1:1,000, mouse monoclonal; Millipore MAB374).

    Techniques: Inhibition, Staining, Microscopy

    Sublethal inhibition of mitochondrial Kv1.3 leads to cell cycle alterations. (A) FACS analysis of cell cycle of PANC-1 cells untreated or treated with clofazimine 1 µM and ShK 100 nM for 24 h. The distribution was determined by staining cells with 50 µg/mL Propidium Iodide and the acquisition was performed by a FACSantoII (Beckton Dickson). The plots are representative of three separated determinations. Light blue peaks represent apoptotic cells, while red peaks represent cells in G1 and G2/M phases. S phase is represented by the area with blue straight lines. A sub-G1 peak is due to dead cells. In the graph of untreated cells the labels and the arrows identify the different populations that are reported. (B) Cell cycle distribution in PANC-1 cells pretreated (lower panels) or not (upper panels) with 50 µM Mitotempo or 20 mM N-acetylcysteine (NAC) for 1 h, before the addition of mitochondrial Kv1.3 inhibitors PAPTP and PCARBTP (both at 100 nM) for 24 h. The experiment and the analysis have been performed as in (A) . The plots are representative of three separate measurements.

    Journal: Frontiers in Oncology

    Article Title: Regulation of Proliferation by a Mitochondrial Potassium Channel in Pancreatic Ductal Adenocarcinoma Cells

    doi: 10.3389/fonc.2017.00239

    Figure Lengend Snippet: Sublethal inhibition of mitochondrial Kv1.3 leads to cell cycle alterations. (A) FACS analysis of cell cycle of PANC-1 cells untreated or treated with clofazimine 1 µM and ShK 100 nM for 24 h. The distribution was determined by staining cells with 50 µg/mL Propidium Iodide and the acquisition was performed by a FACSantoII (Beckton Dickson). The plots are representative of three separated determinations. Light blue peaks represent apoptotic cells, while red peaks represent cells in G1 and G2/M phases. S phase is represented by the area with blue straight lines. A sub-G1 peak is due to dead cells. In the graph of untreated cells the labels and the arrows identify the different populations that are reported. (B) Cell cycle distribution in PANC-1 cells pretreated (lower panels) or not (upper panels) with 50 µM Mitotempo or 20 mM N-acetylcysteine (NAC) for 1 h, before the addition of mitochondrial Kv1.3 inhibitors PAPTP and PCARBTP (both at 100 nM) for 24 h. The experiment and the analysis have been performed as in (A) . The plots are representative of three separate measurements.

    Article Snippet: After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-Kv1.3 (1:200, rabbit polyclonal; Alomone Labs APC-101); anti-GAPDH (1:1,000, mouse monoclonal; Millipore MAB374).

    Techniques: Inhibition, FACS, Staining

    Potassium channel Kv1.3 is expressed in PANC-1 cells. (A) Kv1.3 expression was determined by Western Blot in PANC-1 cells. 60 µg of total protein extract were loaded into a SDS gel and blotted onto a polyvinylidene fluoride (PVDF) membrane. Kv1.3 band was evaluated by immunoblotting with a specific antibody. GAPDH was used as loading control. The blot is a representative image of three different observations. (B) Inhibition of mitochondrial Kv1.3 by different concentration of membrane permeant blockers resulted in a reduction of the MTS signal from PANC-1 cells. Values are reported as percentage respect to untreated sample ± SEM. All compounds were added for 24 h. Staurosporine was used as positive control ( n = 3; *** p

    Journal: Frontiers in Oncology

    Article Title: Regulation of Proliferation by a Mitochondrial Potassium Channel in Pancreatic Ductal Adenocarcinoma Cells

    doi: 10.3389/fonc.2017.00239

    Figure Lengend Snippet: Potassium channel Kv1.3 is expressed in PANC-1 cells. (A) Kv1.3 expression was determined by Western Blot in PANC-1 cells. 60 µg of total protein extract were loaded into a SDS gel and blotted onto a polyvinylidene fluoride (PVDF) membrane. Kv1.3 band was evaluated by immunoblotting with a specific antibody. GAPDH was used as loading control. The blot is a representative image of three different observations. (B) Inhibition of mitochondrial Kv1.3 by different concentration of membrane permeant blockers resulted in a reduction of the MTS signal from PANC-1 cells. Values are reported as percentage respect to untreated sample ± SEM. All compounds were added for 24 h. Staurosporine was used as positive control ( n = 3; *** p

    Article Snippet: After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-Kv1.3 (1:200, rabbit polyclonal; Alomone Labs APC-101); anti-GAPDH (1:1,000, mouse monoclonal; Millipore MAB374).

    Techniques: Expressing, Western Blot, SDS-Gel, Inhibition, Concentration Assay, Positive Control

    Characterization of the Kv1.3 and Kv1.5 mutant channels containing the YS segment. A , average normalized activation and inactivation curves are shown as conductance-voltage relationships for Kv1.3, Kv1.5, the truncated Kv1.3-YS channel, and the chimeras Kv1.5-YS 532 and Kv1.5-YS 613 . All datasets were fitted to Boltzmann functions. Each data point is the mean ± S.E. of 6–11 cells. B , confocal images of non-permeabilized cells transfected with Kv1.3-YS-Cherry, Kv1.5-YS 532 -EGFP, and Kv1.5-YS 613 -EGFP. An extracellular anti-Kv1.3 antibody was used to label Kv1.3-YS ( green ), whereas the extracellular anti-Kv1.5 antibody was used for Kv1.5-YS 532 and Kv1.5-YS 613 chimeras ( red ). Nuclei were stained by Hoechst ( blue ). C , proliferation rate of the indicated channels or GFP-transfected cells (control) was determined by measuring EdU incorporation. Significant differences when comparing to Kv1.3 (*) or to control (#) are indicated. Statistical analysis was performed with one-way ANOVA followed by a Tukey's HSD multiple comparison. Each bar is the average of 9–15 determinations from 5 different assays. D , the average peak current amplitude obtained in cell-attached experiments for Kv1.5 channels and all the Kv1.5 chimeras was plotted against the % of the channels expressed at the plasma membrane ( upper graph ) or their normalized effect on proliferation (taking 100% as the proliferation rate of GFP-transfected HEK cells, lower graph ). The correlation between expression and current was fit to a linear regression curve ( y = 18.54 + 0.0066x, R 2 = 0.85, p = 0.008), but there was no correlation between proliferation and current amplitude ( R 2 = 0.23, p = 0.19).

    Journal: The Journal of Biological Chemistry

    Article Title: Molecular Determinants of Kv1.3 Potassium Channels-induced Proliferation *

    doi: 10.1074/jbc.M115.678995

    Figure Lengend Snippet: Characterization of the Kv1.3 and Kv1.5 mutant channels containing the YS segment. A , average normalized activation and inactivation curves are shown as conductance-voltage relationships for Kv1.3, Kv1.5, the truncated Kv1.3-YS channel, and the chimeras Kv1.5-YS 532 and Kv1.5-YS 613 . All datasets were fitted to Boltzmann functions. Each data point is the mean ± S.E. of 6–11 cells. B , confocal images of non-permeabilized cells transfected with Kv1.3-YS-Cherry, Kv1.5-YS 532 -EGFP, and Kv1.5-YS 613 -EGFP. An extracellular anti-Kv1.3 antibody was used to label Kv1.3-YS ( green ), whereas the extracellular anti-Kv1.5 antibody was used for Kv1.5-YS 532 and Kv1.5-YS 613 chimeras ( red ). Nuclei were stained by Hoechst ( blue ). C , proliferation rate of the indicated channels or GFP-transfected cells (control) was determined by measuring EdU incorporation. Significant differences when comparing to Kv1.3 (*) or to control (#) are indicated. Statistical analysis was performed with one-way ANOVA followed by a Tukey's HSD multiple comparison. Each bar is the average of 9–15 determinations from 5 different assays. D , the average peak current amplitude obtained in cell-attached experiments for Kv1.5 channels and all the Kv1.5 chimeras was plotted against the % of the channels expressed at the plasma membrane ( upper graph ) or their normalized effect on proliferation (taking 100% as the proliferation rate of GFP-transfected HEK cells, lower graph ). The correlation between expression and current was fit to a linear regression curve ( y = 18.54 + 0.0066x, R 2 = 0.85, p = 0.008), but there was no correlation between proliferation and current amplitude ( R 2 = 0.23, p = 0.19).

    Article Snippet: Non-permeabilized cells were incubated with anti-Kv1.3 or anti Kv1.5 extracellular primary antibodies (APC101 or APC150, Alomone Labs), whereas permeabilized cells were incubated with anti-Kv1.3 COOH (75-009, NeuroMab) or anti-Kv1.5 COOH (APC004, Alomone Labs), all at a final concentration of 1:50.

    Techniques: Mutagenesis, Activation Assay, Transfection, Staining, Expressing

    Expression of Kv1.3 in human pancreatic cancer tissue. Expression of Kv1.3 in human pancreatic tissue. Panel ( a ), high expression level of Kv1.3 in human pancreatic cancer tissue. ( n = 33 out of 55, scale bar = 50 µm). Panel ( b ), magnification of panel ( a ), scale bar = 20 µm. Panel ( c ), low expression level of Kv1.3 in human pancreatic cancer tissue. ( n = 22 out of 55, scale bar = 50 µm). Panel ( d ), magnification of panel c, scale bar = 20 µm. Panel ( e ), Immunoglobulin G (IgG) control with magnification (Panel ( f )). Panel ( g ) shows a pancreas adenocarcinoma ductule with high expression of Kv1.3 (brown color) and adjacent normal pancreas parenchyma without Kv1.3 expression (black arrow), scale bar = 50 µm. Panel ( h ), magnification of panel ( g ), scale bar = 20 µm.

    Journal: Cancers

    Article Title: Inhibition of a Mitochondrial Potassium Channel in Combination with Gemcitabine and Abraxane Drastically Reduces Pancreatic Ductal Adenocarcinoma in an Immunocompetent Orthotopic Murine Model

    doi: 10.3390/cancers14112618

    Figure Lengend Snippet: Expression of Kv1.3 in human pancreatic cancer tissue. Expression of Kv1.3 in human pancreatic tissue. Panel ( a ), high expression level of Kv1.3 in human pancreatic cancer tissue. ( n = 33 out of 55, scale bar = 50 µm). Panel ( b ), magnification of panel ( a ), scale bar = 20 µm. Panel ( c ), low expression level of Kv1.3 in human pancreatic cancer tissue. ( n = 22 out of 55, scale bar = 50 µm). Panel ( d ), magnification of panel c, scale bar = 20 µm. Panel ( e ), Immunoglobulin G (IgG) control with magnification (Panel ( f )). Panel ( g ) shows a pancreas adenocarcinoma ductule with high expression of Kv1.3 (brown color) and adjacent normal pancreas parenchyma without Kv1.3 expression (black arrow), scale bar = 50 µm. Panel ( h ), magnification of panel ( g ), scale bar = 20 µm.

    Article Snippet: Primary antibodies Kv1.3 (1:100, APC-101, Alomone labs, Jerusalem, Israel) and TOM20 (1:100, MABT166, Sigma, Burlington, MA, USA) were incubated overnight at 4 °C.

    Techniques: Expressing

    Kv1.3 and KCa3.1 currents are expressed in D10 cells. ( A ): Representative Kv1.3 K + current in a single D10 cell recorded during a 1.5-s-long test pulse to +50 mV from a holding potential of –120 mV. The superimposed dashed line indicates the best fit single exponential with τ =252 ms. ( B ): Representative Kv1.3 K + currents from a single D10 cell in control solution, and after the equilibration of the block in the presence of 15 pM MgTx (test pulse: +50 mV). ( C ): Voltage ramps from –120 mV to +50 mV (duration: 150 ms) evoked KCa3.1 currents from a single D10 cell. Traces show the current in control solution, after the equilibration of the block in the presence of 250 nM TRAM-34, and after wash-out. The voltage range below the activation threshold of Kv1.3 channels is shown only.

    Journal: International Journal of Molecular Sciences

    Article Title: Periodic Membrane Potential and Ca2+ Oscillations in T Cells Forming an Immune Synapse

    doi: 10.3390/ijms21051568

    Figure Lengend Snippet: Kv1.3 and KCa3.1 currents are expressed in D10 cells. ( A ): Representative Kv1.3 K + current in a single D10 cell recorded during a 1.5-s-long test pulse to +50 mV from a holding potential of –120 mV. The superimposed dashed line indicates the best fit single exponential with τ =252 ms. ( B ): Representative Kv1.3 K + currents from a single D10 cell in control solution, and after the equilibration of the block in the presence of 15 pM MgTx (test pulse: +50 mV). ( C ): Voltage ramps from –120 mV to +50 mV (duration: 150 ms) evoked KCa3.1 currents from a single D10 cell. Traces show the current in control solution, after the equilibration of the block in the presence of 250 nM TRAM-34, and after wash-out. The voltage range below the activation threshold of Kv1.3 channels is shown only.

    Article Snippet: The primary antibody was raised against the extracellular loop between S1 and S2 segments of the channel protein (amino acids 211–224: KDYPASTSQDSFEA(C)) (cat #: APC-101, 1:200 dilution, Alomone Labs, Jerusalem, Israel), the secondary antibody was a Cy3 conjugated donkey anti-rabbit IgG (1:500 dilution, The Jackson Laboratory, Sacramento, CA USA).

    Techniques: Blocking Assay, Activation Assay

    Recruitment of PKCθ-GFP and Kv1.3 into the IS. Representative confocal images of a D10 cell alone ( A – D ) or conjugated to a CH12 cell (E-H). Panels from left to right display: ( A , E ): GFP signal of PKCΘ (green), ( B , F ): Cy3 fluorescence of Kv1.3 signal (red), ( C , G ): merge of the PKCθ-GFP and Kv1.3 signals. ( D , H ): bright field image of the cells. Slice thickness was set to 1 μm. The image was taken 20 min after mixing and centrifuging together the two cell types. Scale bar is 10 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Periodic Membrane Potential and Ca2+ Oscillations in T Cells Forming an Immune Synapse

    doi: 10.3390/ijms21051568

    Figure Lengend Snippet: Recruitment of PKCθ-GFP and Kv1.3 into the IS. Representative confocal images of a D10 cell alone ( A – D ) or conjugated to a CH12 cell (E-H). Panels from left to right display: ( A , E ): GFP signal of PKCΘ (green), ( B , F ): Cy3 fluorescence of Kv1.3 signal (red), ( C , G ): merge of the PKCθ-GFP and Kv1.3 signals. ( D , H ): bright field image of the cells. Slice thickness was set to 1 μm. The image was taken 20 min after mixing and centrifuging together the two cell types. Scale bar is 10 µm.

    Article Snippet: The primary antibody was raised against the extracellular loop between S1 and S2 segments of the channel protein (amino acids 211–224: KDYPASTSQDSFEA(C)) (cat #: APC-101, 1:200 dilution, Alomone Labs, Jerusalem, Israel), the secondary antibody was a Cy3 conjugated donkey anti-rabbit IgG (1:500 dilution, The Jackson Laboratory, Sacramento, CA USA).

    Techniques: Fluorescence

    Membrane potential oscillations are sensitive to Kv1.3 and KCa3.1 channel blockers and to extracellular calcium concentration. ( A , B ): Representative membrane potential records (current-clamp) of D10 cells conjugated to CH12 cells. MPO was by induced by depolarizing current injections (arrows) with durations indicated by the lengths of the horizontal lines. The hatched horizontal bar indicates perfusion with 50 nM ChTx ( A ) or with 250 nM TRAM-34 ( B ). ( C ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following the perfusion with 50 nM ChTx (ChTx). ( D ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following or 250 nM TRAM-34 (TRAM-34). Oblique lines connect the corresponding average amplitudes in Ctrl and in the presence of the blockers. ( E ): Effect of ChTx and TRAM-34 on the MPO amplitudes. The MPO amplitudes (Amp) were pooled before (Ctrl ChTx) and after 50 nM ChTx (ChTX) or before (Ctrl TRAM-34) and after 250 nM TRAM-34 (TRAM-34) and averaged (mean ± SEM). Asterisks indicate significant differences (* p

    Journal: International Journal of Molecular Sciences

    Article Title: Periodic Membrane Potential and Ca2+ Oscillations in T Cells Forming an Immune Synapse

    doi: 10.3390/ijms21051568

    Figure Lengend Snippet: Membrane potential oscillations are sensitive to Kv1.3 and KCa3.1 channel blockers and to extracellular calcium concentration. ( A , B ): Representative membrane potential records (current-clamp) of D10 cells conjugated to CH12 cells. MPO was by induced by depolarizing current injections (arrows) with durations indicated by the lengths of the horizontal lines. The hatched horizontal bar indicates perfusion with 50 nM ChTx ( A ) or with 250 nM TRAM-34 ( B ). ( C ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following the perfusion with 50 nM ChTx (ChTx). ( D ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following or 250 nM TRAM-34 (TRAM-34). Oblique lines connect the corresponding average amplitudes in Ctrl and in the presence of the blockers. ( E ): Effect of ChTx and TRAM-34 on the MPO amplitudes. The MPO amplitudes (Amp) were pooled before (Ctrl ChTx) and after 50 nM ChTx (ChTX) or before (Ctrl TRAM-34) and after 250 nM TRAM-34 (TRAM-34) and averaged (mean ± SEM). Asterisks indicate significant differences (* p

    Article Snippet: The primary antibody was raised against the extracellular loop between S1 and S2 segments of the channel protein (amino acids 211–224: KDYPASTSQDSFEA(C)) (cat #: APC-101, 1:200 dilution, Alomone Labs, Jerusalem, Israel), the secondary antibody was a Cy3 conjugated donkey anti-rabbit IgG (1:500 dilution, The Jackson Laboratory, Sacramento, CA USA).

    Techniques: Concentration Assay