ikca  (Alomone Labs)

Journal: PLOS ONE

Article Title: Peptide Lv augments intermediate-conductance calcium-dependent potassium channels (KCa3.1) in endothelial cells to promote angiogenesis

doi: 10.1371/journal.pone.0276744

Figure Lengend Snippet: Peptide Lv increases the mRNA and protein levels of K Ca 3.1 ( Kcnn4 ) in ECs. Endothelial cells were treated with PBS (vehicle control) or peptide Lv (500 ng/ml) for 4 hours and processed for qPCR or immunoblotting. Peptide Lv increased the mRNA (A) and protein (B) levels of K Ca 3.1 in cultured HUVECs. (C) Peptide Lv also increased the protein level of K Ca 3.1 in HRECs. Student’s t -test was used for statistical analyses; * p

Article Snippet: The primary antibodies used were rabbit polyclonal anti-KCNN4, (KCa 3.1, #APC-0641:200; Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-KCNN3 (KCa2.3, #APC-025; 1:200; Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-Kir6.1 (1:500; #NBP1-87710, Novus Biologicals, Littleton, CO, USA), and rabbit monoclonal β-actin (1:1000; #4970S, Cell Signaling Technologies, Danvers, MA, USA).

Techniques: Real-time Polymerase Chain Reaction, Cell Culture

Journal: Open Life Sciences

Article Title: Effects of KCa channels on biological behavior of trophoblasts

doi: 10.1515/biol-2022-0462

Figure Lengend Snippet: Expression of KCa channels in human villus tissues. Immunohistochemical detection of different types of KCa channels (BKCa α, BKCa β1, IKCa, and SKCa) and the negative control in human villus tissues.

Article Snippet: Then, cells were permeabilized with 0.5% TritonX-100 for 5 min before being blocked with 5% BSA for 1 h. The coverslips were incubated with primary antibody against cytokeratin 7 (CK7, 1:50; Abcam, UK) and anti-vimentin (1:50; Abcam), which were used for the confirmation of EVTs, as well as with other primary antibodies against BKCa α (1:50; Alomone Labs), BKCa β1 (1:50; Alomone Labs), IKCa (1:50; Alomone Labs), and SKCa (1:50; Alomone Labs) at 4°C overnight.

Techniques: Expressing, Immunohistochemistry, Negative Control

Journal: Open Life Sciences

Article Title: Effects of KCa channels on biological behavior of trophoblasts

doi: 10.1515/biol-2022-0462

Figure Lengend Snippet: Localization and expression of KCa channels in HTR-8/SVneo cells. BKCa α and β1 subunits, IKCa channels, SKCa channels, and the control in HTR-8/SVneo cells were observed under the fluorescence microscope. Cells were stained with DAPI to visualize nucleus (blue) and immunolabeled with Anti-KCa antibodies (green), respectively. Arrow indicates the positive staining.

Article Snippet: Then, cells were permeabilized with 0.5% TritonX-100 for 5 min before being blocked with 5% BSA for 1 h. The coverslips were incubated with primary antibody against cytokeratin 7 (CK7, 1:50; Abcam, UK) and anti-vimentin (1:50; Abcam), which were used for the confirmation of EVTs, as well as with other primary antibodies against BKCa α (1:50; Alomone Labs), BKCa β1 (1:50; Alomone Labs), IKCa (1:50; Alomone Labs), and SKCa (1:50; Alomone Labs) at 4°C overnight.

Techniques: Expressing, Fluorescence, Microscopy, Staining, Immunolabeling