ikca  (Alomone Labs)


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    Structured Review

    Alomone Labs ikca
    Expression of KCa channels in human villus tissues. Immunohistochemical detection of different types of KCa <t>channels</t> <t>(BKCa</t> α, BKCa β1, <t>IKCa,</t> and SKCa) and the negative control in human villus tissues.
    Ikca, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ikca/product/Alomone Labs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ikca - by Bioz Stars, 2022-12
    93/100 stars

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    1) Product Images from "Effects of KCa channels on biological behavior of trophoblasts"

    Article Title: Effects of KCa channels on biological behavior of trophoblasts

    Journal: Open Life Sciences

    doi: 10.1515/biol-2022-0462

    Expression of KCa channels in human villus tissues. Immunohistochemical detection of different types of KCa channels (BKCa α, BKCa β1, IKCa, and SKCa) and the negative control in human villus tissues.
    Figure Legend Snippet: Expression of KCa channels in human villus tissues. Immunohistochemical detection of different types of KCa channels (BKCa α, BKCa β1, IKCa, and SKCa) and the negative control in human villus tissues.

    Techniques Used: Expressing, Immunohistochemistry, Negative Control

    Localization and expression of KCa channels in HTR-8/SVneo cells. BKCa α and β1 subunits, IKCa channels, SKCa channels, and the control in HTR-8/SVneo cells were observed under the fluorescence microscope. Cells were stained with DAPI to visualize nucleus (blue) and immunolabeled with Anti-KCa antibodies (green), respectively. Arrow indicates the positive staining.
    Figure Legend Snippet: Localization and expression of KCa channels in HTR-8/SVneo cells. BKCa α and β1 subunits, IKCa channels, SKCa channels, and the control in HTR-8/SVneo cells were observed under the fluorescence microscope. Cells were stained with DAPI to visualize nucleus (blue) and immunolabeled with Anti-KCa antibodies (green), respectively. Arrow indicates the positive staining.

    Techniques Used: Expressing, Fluorescence, Microscopy, Staining, Immunolabeling

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    Alomone Labs kca 3 1
    Peptide Lv increases the mRNA and protein levels of K Ca 3.1 ( <t>Kcnn4</t> ) in ECs. Endothelial cells were treated with PBS (vehicle control) or peptide Lv (500 ng/ml) for 4 hours and processed for qPCR or immunoblotting. Peptide Lv increased the mRNA (A) and protein (B) levels of K Ca 3.1 in cultured HUVECs. (C) Peptide Lv also increased the protein level of K Ca 3.1 in HRECs. Student’s t -test was used for statistical analyses; * p
    Kca 3 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kca 3 1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kca 3 1 - by Bioz Stars, 2022-12
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    90
    Alomone Labs anti kcnn4 kca3 1 sk4 antibody
    Peptide Lv increases the mRNA and protein levels of K Ca 3.1 ( <t>Kcnn4</t> ) in ECs. Endothelial cells were treated with PBS (vehicle control) or peptide Lv (500 ng/ml) for 4 hours and processed for qPCR or immunoblotting. Peptide Lv increased the mRNA (A) and protein (B) levels of K Ca 3.1 in cultured HUVECs. (C) Peptide Lv also increased the protein level of K Ca 3.1 in HRECs. Student’s t -test was used for statistical analyses; * p
    Anti Kcnn4 Kca3 1 Sk4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kcnn4 kca3 1 sk4 antibody/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kcnn4 kca3 1 sk4 antibody - by Bioz Stars, 2022-12
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    Peptide Lv increases the mRNA and protein levels of K Ca 3.1 ( Kcnn4 ) in ECs. Endothelial cells were treated with PBS (vehicle control) or peptide Lv (500 ng/ml) for 4 hours and processed for qPCR or immunoblotting. Peptide Lv increased the mRNA (A) and protein (B) levels of K Ca 3.1 in cultured HUVECs. (C) Peptide Lv also increased the protein level of K Ca 3.1 in HRECs. Student’s t -test was used for statistical analyses; * p

    Journal: PLOS ONE

    Article Title: Peptide Lv augments intermediate-conductance calcium-dependent potassium channels (KCa3.1) in endothelial cells to promote angiogenesis

    doi: 10.1371/journal.pone.0276744

    Figure Lengend Snippet: Peptide Lv increases the mRNA and protein levels of K Ca 3.1 ( Kcnn4 ) in ECs. Endothelial cells were treated with PBS (vehicle control) or peptide Lv (500 ng/ml) for 4 hours and processed for qPCR or immunoblotting. Peptide Lv increased the mRNA (A) and protein (B) levels of K Ca 3.1 in cultured HUVECs. (C) Peptide Lv also increased the protein level of K Ca 3.1 in HRECs. Student’s t -test was used for statistical analyses; * p

    Article Snippet: The primary antibodies used were rabbit polyclonal anti-KCNN4, (KCa 3.1, #APC-0641:200; Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-KCNN3 (KCa2.3, #APC-025; 1:200; Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-Kir6.1 (1:500; #NBP1-87710, Novus Biologicals, Littleton, CO, USA), and rabbit monoclonal β-actin (1:1000; #4970S, Cell Signaling Technologies, Danvers, MA, USA).

    Techniques: Real-time Polymerase Chain Reaction, Cell Culture

    Expression of KCa channels in human villus tissues. Immunohistochemical detection of different types of KCa channels (BKCa α, BKCa β1, IKCa, and SKCa) and the negative control in human villus tissues.

    Journal: Open Life Sciences

    Article Title: Effects of KCa channels on biological behavior of trophoblasts

    doi: 10.1515/biol-2022-0462

    Figure Lengend Snippet: Expression of KCa channels in human villus tissues. Immunohistochemical detection of different types of KCa channels (BKCa α, BKCa β1, IKCa, and SKCa) and the negative control in human villus tissues.

    Article Snippet: Then, cells were permeabilized with 0.5% TritonX-100 for 5 min before being blocked with 5% BSA for 1 h. The coverslips were incubated with primary antibody against cytokeratin 7 (CK7, 1:50; Abcam, UK) and anti-vimentin (1:50; Abcam), which were used for the confirmation of EVTs, as well as with other primary antibodies against BKCa α (1:50; Alomone Labs), BKCa β1 (1:50; Alomone Labs), IKCa (1:50; Alomone Labs), and SKCa (1:50; Alomone Labs) at 4°C overnight.

    Techniques: Expressing, Immunohistochemistry, Negative Control

    Localization and expression of KCa channels in HTR-8/SVneo cells. BKCa α and β1 subunits, IKCa channels, SKCa channels, and the control in HTR-8/SVneo cells were observed under the fluorescence microscope. Cells were stained with DAPI to visualize nucleus (blue) and immunolabeled with Anti-KCa antibodies (green), respectively. Arrow indicates the positive staining.

    Journal: Open Life Sciences

    Article Title: Effects of KCa channels on biological behavior of trophoblasts

    doi: 10.1515/biol-2022-0462

    Figure Lengend Snippet: Localization and expression of KCa channels in HTR-8/SVneo cells. BKCa α and β1 subunits, IKCa channels, SKCa channels, and the control in HTR-8/SVneo cells were observed under the fluorescence microscope. Cells were stained with DAPI to visualize nucleus (blue) and immunolabeled with Anti-KCa antibodies (green), respectively. Arrow indicates the positive staining.

    Article Snippet: Then, cells were permeabilized with 0.5% TritonX-100 for 5 min before being blocked with 5% BSA for 1 h. The coverslips were incubated with primary antibody against cytokeratin 7 (CK7, 1:50; Abcam, UK) and anti-vimentin (1:50; Abcam), which were used for the confirmation of EVTs, as well as with other primary antibodies against BKCa α (1:50; Alomone Labs), BKCa β1 (1:50; Alomone Labs), IKCa (1:50; Alomone Labs), and SKCa (1:50; Alomone Labs) at 4°C overnight.

    Techniques: Expressing, Fluorescence, Microscopy, Staining, Immunolabeling