hcn1  (Alomone Labs)


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    Alomone Labs hcn1
    Hcn1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcn1/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn1 - by Bioz Stars, 2022-12
    90/100 stars

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    Alomone Labs anti hcn1 antibody
    Electrophysiological properties of novel and de novo variants in <t>HCN1</t> located in or close to transmembrane regions. (A) Representative recordings of currents mediated by mutant HCN1 channels. (B) Current densities of E240G ( n = 16), I380F ( n = 9), and A395G channels ( n = 8) (WT1.0: n = 9). Dunnett’s T3 post hoc test. (C) Activation curves of WT and E240G channels (WT: n = 12; E240G: n = 11), and lines represent Boltzmann functions fit to the data points. (D) Mean time constants of activation obtained for HCN-WT (black) and E240G channels (red). (WT: n = 8; E240G: n = 15). Dunnett’s T3 post hoc test. (E) Bar graph shows mean deactivation time constants of WT and E240G channels recorded at + 10 mV (WT: n = 8; E240G: n = 12). Two-sample t -test. (F) Representative traces of heterozygous channels. (G) Plot of mean current densities of WT/E240G ( n = 13), WT/I380F ( n = 13), WT/A395G ( n = 12), and WT channels ( n = 18). Dunnett’s T3 post hoc test. (H) Activation curves of WT channels ( n = 6, black), WT/I380F channels ( n = 6, blue), WT/E240G ( n = 11, red), and WT/A395G channels ( n = 12, orange). Bonferroni post hoc test. (I,J) Graphs show the effects of the activation (WT/E240G: n = 17; WT/I380F: n = 12; WT/A395G: n = 19; WT0.5: n = 12) and deactivation time constants (WT/I380F: n = 12; WT/E240G: n = 17; WT/A395G n = 17; WT0.5 n = 14) induced by mutant heterozygous channels. Bonferroni post hoc test. All data are presented as mean ± S.E.M. values (n.s. = not significant, * P
    Anti Hcn1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hcn1 antibody/product/Alomone Labs
    Average 95 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti hcn1 antibody - by Bioz Stars, 2022-12
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    Electrophysiological properties of novel and de novo variants in HCN1 located in or close to transmembrane regions. (A) Representative recordings of currents mediated by mutant HCN1 channels. (B) Current densities of E240G ( n = 16), I380F ( n = 9), and A395G channels ( n = 8) (WT1.0: n = 9). Dunnett’s T3 post hoc test. (C) Activation curves of WT and E240G channels (WT: n = 12; E240G: n = 11), and lines represent Boltzmann functions fit to the data points. (D) Mean time constants of activation obtained for HCN-WT (black) and E240G channels (red). (WT: n = 8; E240G: n = 15). Dunnett’s T3 post hoc test. (E) Bar graph shows mean deactivation time constants of WT and E240G channels recorded at + 10 mV (WT: n = 8; E240G: n = 12). Two-sample t -test. (F) Representative traces of heterozygous channels. (G) Plot of mean current densities of WT/E240G ( n = 13), WT/I380F ( n = 13), WT/A395G ( n = 12), and WT channels ( n = 18). Dunnett’s T3 post hoc test. (H) Activation curves of WT channels ( n = 6, black), WT/I380F channels ( n = 6, blue), WT/E240G ( n = 11, red), and WT/A395G channels ( n = 12, orange). Bonferroni post hoc test. (I,J) Graphs show the effects of the activation (WT/E240G: n = 17; WT/I380F: n = 12; WT/A395G: n = 19; WT0.5: n = 12) and deactivation time constants (WT/I380F: n = 12; WT/E240G: n = 17; WT/A395G n = 17; WT0.5 n = 14) induced by mutant heterozygous channels. Bonferroni post hoc test. All data are presented as mean ± S.E.M. values (n.s. = not significant, * P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Novel HCN1 Mutations Associated With Epilepsy and Impacts on Neuronal Excitability

    doi: 10.3389/fnmol.2022.870182

    Figure Lengend Snippet: Electrophysiological properties of novel and de novo variants in HCN1 located in or close to transmembrane regions. (A) Representative recordings of currents mediated by mutant HCN1 channels. (B) Current densities of E240G ( n = 16), I380F ( n = 9), and A395G channels ( n = 8) (WT1.0: n = 9). Dunnett’s T3 post hoc test. (C) Activation curves of WT and E240G channels (WT: n = 12; E240G: n = 11), and lines represent Boltzmann functions fit to the data points. (D) Mean time constants of activation obtained for HCN-WT (black) and E240G channels (red). (WT: n = 8; E240G: n = 15). Dunnett’s T3 post hoc test. (E) Bar graph shows mean deactivation time constants of WT and E240G channels recorded at + 10 mV (WT: n = 8; E240G: n = 12). Two-sample t -test. (F) Representative traces of heterozygous channels. (G) Plot of mean current densities of WT/E240G ( n = 13), WT/I380F ( n = 13), WT/A395G ( n = 12), and WT channels ( n = 18). Dunnett’s T3 post hoc test. (H) Activation curves of WT channels ( n = 6, black), WT/I380F channels ( n = 6, blue), WT/E240G ( n = 11, red), and WT/A395G channels ( n = 12, orange). Bonferroni post hoc test. (I,J) Graphs show the effects of the activation (WT/E240G: n = 17; WT/I380F: n = 12; WT/A395G: n = 19; WT0.5: n = 12) and deactivation time constants (WT/I380F: n = 12; WT/E240G: n = 17; WT/A395G n = 17; WT0.5 n = 14) induced by mutant heterozygous channels. Bonferroni post hoc test. All data are presented as mean ± S.E.M. values (n.s. = not significant, * P

    Article Snippet: They were next incubated with anti-HCN1 antibody (Alomone, 1:500, overnight, 4°C) followed by secondary antibodies (anti-rabbit Cy3, Jackson lab, 6 h, room temperature).

    Techniques: Mutagenesis, Activation Assay

    Neuronal excitability was affected by HCN1 variants. (A) Representative recordings of action potential firing patterns in response to current injection (180 pA). (B) Bar graph summarizes the mean membrane potential in neurons expressing different HCN1 channel types. Analysis was done by Bonferroni post hoc test. (C) Bar graph shows mean input resistance in neurons expressing different HCN1 channel types. Dunnett’s T3 post hoc test. (D) Bar graph shows spike thresholds elicited by current injection were not affected. Analysis was done by Mann–Whitney test. (E) Plot of the mean firing rates of neurons in response to current injections. * P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Novel HCN1 Mutations Associated With Epilepsy and Impacts on Neuronal Excitability

    doi: 10.3389/fnmol.2022.870182

    Figure Lengend Snippet: Neuronal excitability was affected by HCN1 variants. (A) Representative recordings of action potential firing patterns in response to current injection (180 pA). (B) Bar graph summarizes the mean membrane potential in neurons expressing different HCN1 channel types. Analysis was done by Bonferroni post hoc test. (C) Bar graph shows mean input resistance in neurons expressing different HCN1 channel types. Dunnett’s T3 post hoc test. (D) Bar graph shows spike thresholds elicited by current injection were not affected. Analysis was done by Mann–Whitney test. (E) Plot of the mean firing rates of neurons in response to current injections. * P

    Article Snippet: They were next incubated with anti-HCN1 antibody (Alomone, 1:500, overnight, 4°C) followed by secondary antibodies (anti-rabbit Cy3, Jackson lab, 6 h, room temperature).

    Techniques: Injection, Expressing, MANN-WHITNEY

    EEG of four patients carrying HCN1 variants. (A) EEG of patient 2 (I380F) shows sharp waves in the left hemisphere during sleep (top) and spike waves during status epilepticus (bottom). Gray arrows indicate abnormal EEG waves. (B) EEG of patient 3 (A395G) shows sharp and sharp slow waves in the right occipital region during sleep (bottom). (C) EEG of patient 4 (S710Rfs*71) features a slow rhythm of background in the occipital region (top) and a paroxysm of diffused 4–5 Hz slow waves (bottom). (D) EEG of patient 5 (V572A) shows θ rhythm mixed δ waves in the background (top) and diffused 3.5–4 Hz multifocal spike-slow waves during a tonic-clonic seizure, while the patient was having a tonic-clonic seizure in sleep ( Figure 2D ) (bottom).

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Novel HCN1 Mutations Associated With Epilepsy and Impacts on Neuronal Excitability

    doi: 10.3389/fnmol.2022.870182

    Figure Lengend Snippet: EEG of four patients carrying HCN1 variants. (A) EEG of patient 2 (I380F) shows sharp waves in the left hemisphere during sleep (top) and spike waves during status epilepticus (bottom). Gray arrows indicate abnormal EEG waves. (B) EEG of patient 3 (A395G) shows sharp and sharp slow waves in the right occipital region during sleep (bottom). (C) EEG of patient 4 (S710Rfs*71) features a slow rhythm of background in the occipital region (top) and a paroxysm of diffused 4–5 Hz slow waves (bottom). (D) EEG of patient 5 (V572A) shows θ rhythm mixed δ waves in the background (top) and diffused 3.5–4 Hz multifocal spike-slow waves during a tonic-clonic seizure, while the patient was having a tonic-clonic seizure in sleep ( Figure 2D ) (bottom).

    Article Snippet: They were next incubated with anti-HCN1 antibody (Alomone, 1:500, overnight, 4°C) followed by secondary antibodies (anti-rabbit Cy3, Jackson lab, 6 h, room temperature).

    Techniques:

    Functional changes of the S710Rfs*71 variant. (A) Representative recordings of currents mediated by HCN1 channels. (B) Plot of mean current densities (pA/pF) of WT, S710Rfs*71, and WT/S710Rf*71 channels (WT: n = 14; S710Rfs*71: n = 20; WT/S710Rfs*71: n = 16). (C) Activation curves obtained by normalizing the tail currents of WT, S710Rfs*71, and WT/S710Rf*71 channels (WT: n = 11; S710Rfs*71: n = 13; WT/S710Rf*71: n = 7). (D) Plot of mean activation time constants obtained for HCN1-WT, S710Rfs*71, and WT/S710Rf*71 channels (WT: n = 16; S710Rfs*71: n = 16; WT/S710Rf*71: n = 6). (E) Bar graph shows mean deactivation time constants obtained for HCN1-WT, S710Rfs*71, and WT/S710Rf*71 channels (WT: n = 12; S710Rfs*71: n = 16; WT/S710Rfs*71: n = 6). All the statistical analyses in this figure were performed by one-way ANOVA and Bonferroni post hoc test. * P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Novel HCN1 Mutations Associated With Epilepsy and Impacts on Neuronal Excitability

    doi: 10.3389/fnmol.2022.870182

    Figure Lengend Snippet: Functional changes of the S710Rfs*71 variant. (A) Representative recordings of currents mediated by HCN1 channels. (B) Plot of mean current densities (pA/pF) of WT, S710Rfs*71, and WT/S710Rf*71 channels (WT: n = 14; S710Rfs*71: n = 20; WT/S710Rfs*71: n = 16). (C) Activation curves obtained by normalizing the tail currents of WT, S710Rfs*71, and WT/S710Rf*71 channels (WT: n = 11; S710Rfs*71: n = 13; WT/S710Rf*71: n = 7). (D) Plot of mean activation time constants obtained for HCN1-WT, S710Rfs*71, and WT/S710Rf*71 channels (WT: n = 16; S710Rfs*71: n = 16; WT/S710Rf*71: n = 6). (E) Bar graph shows mean deactivation time constants obtained for HCN1-WT, S710Rfs*71, and WT/S710Rf*71 channels (WT: n = 12; S710Rfs*71: n = 16; WT/S710Rfs*71: n = 6). All the statistical analyses in this figure were performed by one-way ANOVA and Bonferroni post hoc test. * P

    Article Snippet: They were next incubated with anti-HCN1 antibody (Alomone, 1:500, overnight, 4°C) followed by secondary antibodies (anti-rabbit Cy3, Jackson lab, 6 h, room temperature).

    Techniques: Functional Assay, Variant Assay, Activation Assay

    Mutant HCN1 channels distributed differently in cortical neurons. (A–E) The expression of WT ( n = 13), E240G ( n = 7), I380F ( n = 9), A395G ( n = 9), and S710Rfs*71 ( n = 9) channels in cortical neurons, respectively.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Novel HCN1 Mutations Associated With Epilepsy and Impacts on Neuronal Excitability

    doi: 10.3389/fnmol.2022.870182

    Figure Lengend Snippet: Mutant HCN1 channels distributed differently in cortical neurons. (A–E) The expression of WT ( n = 13), E240G ( n = 7), I380F ( n = 9), A395G ( n = 9), and S710Rfs*71 ( n = 9) channels in cortical neurons, respectively.

    Article Snippet: They were next incubated with anti-HCN1 antibody (Alomone, 1:500, overnight, 4°C) followed by secondary antibodies (anti-rabbit Cy3, Jackson lab, 6 h, room temperature).

    Techniques: Mutagenesis, Expressing

    Mutations in HCN1 channels. (A) Structure of HCN1 channels with S1-S6 segments and N/C-terminal region. (B) Pedigrees of patients with HCN1 variants. (C) All variants are highly conserved among different species.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Novel HCN1 Mutations Associated With Epilepsy and Impacts on Neuronal Excitability

    doi: 10.3389/fnmol.2022.870182

    Figure Lengend Snippet: Mutations in HCN1 channels. (A) Structure of HCN1 channels with S1-S6 segments and N/C-terminal region. (B) Pedigrees of patients with HCN1 variants. (C) All variants are highly conserved among different species.

    Article Snippet: They were next incubated with anti-HCN1 antibody (Alomone, 1:500, overnight, 4°C) followed by secondary antibodies (anti-rabbit Cy3, Jackson lab, 6 h, room temperature).

    Techniques:

    Hyperpolarizing voltage sag in cortical neurons expressing WT and mutant channels. (A) Representative recordings of voltage sag in response to a −100 pA hyperpolarizing current injection in WT and mutant HCN1 -transfected neurons. (B) Bar graph shows mean voltage sag ratios for different types of HCN1 channels. *** P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Novel HCN1 Mutations Associated With Epilepsy and Impacts on Neuronal Excitability

    doi: 10.3389/fnmol.2022.870182

    Figure Lengend Snippet: Hyperpolarizing voltage sag in cortical neurons expressing WT and mutant channels. (A) Representative recordings of voltage sag in response to a −100 pA hyperpolarizing current injection in WT and mutant HCN1 -transfected neurons. (B) Bar graph shows mean voltage sag ratios for different types of HCN1 channels. *** P

    Article Snippet: They were next incubated with anti-HCN1 antibody (Alomone, 1:500, overnight, 4°C) followed by secondary antibodies (anti-rabbit Cy3, Jackson lab, 6 h, room temperature).

    Techniques: Expressing, Mutagenesis, Injection, Transfection

    HCN channels are present on GABAergic terminals in the mPFC. (A) Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrate the cells in layers 5–6 of the mPFC. Scale bar: 40 µm. (B,C) Single-plane confocal images showing the HCN1-ir (B1), HCN2-ir (B2), HCN4-ir (B3), and GAD65-ir (C1-C3) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). (D) Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bar: 20 µm.

    Journal: Biology Open

    Article Title: Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex

    doi: 10.1242/bio.058840

    Figure Lengend Snippet: HCN channels are present on GABAergic terminals in the mPFC. (A) Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrate the cells in layers 5–6 of the mPFC. Scale bar: 40 µm. (B,C) Single-plane confocal images showing the HCN1-ir (B1), HCN2-ir (B2), HCN4-ir (B3), and GAD65-ir (C1-C3) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). (D) Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bar: 20 µm.

    Article Snippet: Sequential primary immunolabeling for HCN1, HCN2 or HCN4 was performed using anti-HCN1, HCN2 or HCN4 rabbit antibodies (1:40; Alomone Laboratories, Israel, Product# APC-056, APC-030, APC-057) ( ).

    Techniques: Staining, Marker, Labeling

    HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layers 5–6 of mPFC. (A–C) Microscopic confocal images showing HCN1-ir (A), HCN2-ir (B), and HCN4-ir (C) locate in PV-ir interneuron in layers 5–6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bars: 20 µm. (D) High-magnification confocal microscopy images showing that HCN1-ir localize in the soma (d1) and along neurite (d2-d3) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite (d1) is digitally magnified for a better view of neurite in (d2) and (d3), respectively. Scale bars: 20 µm in (d1) and 1 µm in (d2) and (d3). (E) High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digitally magnified for a better view of neurite in (e2). Scale bars: 20 µm in (e1) and 1 µm in (e2).

    Journal: Biology Open

    Article Title: Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex

    doi: 10.1242/bio.058840

    Figure Lengend Snippet: HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layers 5–6 of mPFC. (A–C) Microscopic confocal images showing HCN1-ir (A), HCN2-ir (B), and HCN4-ir (C) locate in PV-ir interneuron in layers 5–6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bars: 20 µm. (D) High-magnification confocal microscopy images showing that HCN1-ir localize in the soma (d1) and along neurite (d2-d3) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite (d1) is digitally magnified for a better view of neurite in (d2) and (d3), respectively. Scale bars: 20 µm in (d1) and 1 µm in (d2) and (d3). (E) High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digitally magnified for a better view of neurite in (e2). Scale bars: 20 µm in (e1) and 1 µm in (e2).

    Article Snippet: Sequential primary immunolabeling for HCN1, HCN2 or HCN4 was performed using anti-HCN1, HCN2 or HCN4 rabbit antibodies (1:40; Alomone Laboratories, Israel, Product# APC-056, APC-030, APC-057) ( ).

    Techniques: Expressing, Staining, Labeling, Confocal Microscopy

    Conditional deletion of Atoh1 from the En1 domain reduces the density of excitatory cerebellar cell types, but increases the density of inhibitory cerebellar cell types. ( A ) En1 Cre/+ ;Atoh1 fl/- mice lack differentiated granule cells, identified with GABARα6. ( B and C ) En1 Cre/+ ;Atoh1 fl/- mice have a reduction in unipolar brush cells, identified by Calretinin and Tbr2. ( D ) En1 Cre/+ ;Atoh1 fl/- mice have dense staining for NFH that marks Purkinje cells and excitatory nuclei (interposed nucleus shown here). ( E through H ) En1 Cre/+ ;Atoh1 fl/- mice have a high density of inhibitory neurons, revealed with the expression of RORα ( E ), HCN1 ( F ), Neurogranin ( G ), and PV ( H ). All images are representative for N=3 brains for each genotype and were performed in P14 mice.

    Journal: eLife

    Article Title: Maturation of Purkinje cell firing properties relies on neurogenesis of excitatory neurons

    doi: 10.7554/eLife.68045

    Figure Lengend Snippet: Conditional deletion of Atoh1 from the En1 domain reduces the density of excitatory cerebellar cell types, but increases the density of inhibitory cerebellar cell types. ( A ) En1 Cre/+ ;Atoh1 fl/- mice lack differentiated granule cells, identified with GABARα6. ( B and C ) En1 Cre/+ ;Atoh1 fl/- mice have a reduction in unipolar brush cells, identified by Calretinin and Tbr2. ( D ) En1 Cre/+ ;Atoh1 fl/- mice have dense staining for NFH that marks Purkinje cells and excitatory nuclei (interposed nucleus shown here). ( E through H ) En1 Cre/+ ;Atoh1 fl/- mice have a high density of inhibitory neurons, revealed with the expression of RORα ( E ), HCN1 ( F ), Neurogranin ( G ), and PV ( H ). All images are representative for N=3 brains for each genotype and were performed in P14 mice.

    Article Snippet: The following primary antibodies were used for the data described in this manuscript: guinea pig (gp)-α-Calbindin (1:1,000; SySy; #214004); rabbit (rb)-α-gamma-aminobutyric acid receptor α6 (GABARα6; 1:500; Millipore Sigma; #AB5610), rb-α-T-box brain protein 2 (Tbr2; 1:500; Abcam; #AB23345), mouse (ms)-α-Calretinin (1:500; Swant; #6B3); ms-α-Neurofilament Heavy (NFH; 1:1,000; Biolegend; #801701); rb-α-Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 1 (HCN1; 1:500; Alomone Lab; #APC-056); goat (gt)-α-RAR-related orphan receptor alpha (RORα; 1:250; Santa Cruz; #F2510); rb-α-parvalbumin (PV; 1:1,000; Swant; #PV25); rb-α-neurogranin (1:500; Millipore Sigma; #AB5620); ms-α-ZebrinII (1:500; kind gift from Dr. Richard Hawkes, University of Calgary, Calgary, Alberta, Canada); rb-α- PLCβ4 (1:150; Santa Cruz Biotechnology; catalog #sc-20760); rb-α-Vglut1 (1:500; SySy; #135302); rb-α-Vglut2 (1:500; SySy; #135403).

    Techniques: Mouse Assay, Staining, Expressing