guinea pig anti hcn4 antibody  (Alomone Labs)


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    Alomone Labs guinea pig anti hcn4 antibody
    Co-labeling of Ca v 1.3 and <t>HCN4</t> in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (red) and HCN4 (green) in the isolated cranial SAN-RA preparation. ( B ) Left panels: close-up views of the area circled in ( A ); right panels: enlargement with cells expressing Ca v 1.3 or HCN4, from boxed region in left panel. ( C ) Immunolabeling of regions co-expressing Ca v 1.3 and HCN4. The left panel shows the region in accordance with the posterior nodal extension (PNL). The yellow rectangle identifies the region considered for close-up views shown in the left panels. Left panels, from top to bottom, indicate corresponding Ca v 1.3 and HCN4 immunostaining. Yellow arrows indicate SANC co-expressing Ca v 1.3 and HCN4.
    Guinea Pig Anti Hcn4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti hcn4 antibody/product/Alomone Labs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti hcn4 antibody - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells"

    Article Title: L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells

    Journal: Cells

    doi: 10.3390/cells11071114

    Co-labeling of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (red) and HCN4 (green) in the isolated cranial SAN-RA preparation. ( B ) Left panels: close-up views of the area circled in ( A ); right panels: enlargement with cells expressing Ca v 1.3 or HCN4, from boxed region in left panel. ( C ) Immunolabeling of regions co-expressing Ca v 1.3 and HCN4. The left panel shows the region in accordance with the posterior nodal extension (PNL). The yellow rectangle identifies the region considered for close-up views shown in the left panels. Left panels, from top to bottom, indicate corresponding Ca v 1.3 and HCN4 immunostaining. Yellow arrows indicate SANC co-expressing Ca v 1.3 and HCN4.
    Figure Legend Snippet: Co-labeling of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (red) and HCN4 (green) in the isolated cranial SAN-RA preparation. ( B ) Left panels: close-up views of the area circled in ( A ); right panels: enlargement with cells expressing Ca v 1.3 or HCN4, from boxed region in left panel. ( C ) Immunolabeling of regions co-expressing Ca v 1.3 and HCN4. The left panel shows the region in accordance with the posterior nodal extension (PNL). The yellow rectangle identifies the region considered for close-up views shown in the left panels. Left panels, from top to bottom, indicate corresponding Ca v 1.3 and HCN4 immunostaining. Yellow arrows indicate SANC co-expressing Ca v 1.3 and HCN4.

    Techniques Used: Labeling, Immunolabeling, Isolation, Expressing, Immunostaining

    Expression of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (green) and HCN4 (red) in n = 64 isolated SANC with distribution of SANC based on Ca v 1.3 or HCN4 fluorescence intensity. Low fluorescence intensity is related to a lower expression of the channels while high density is related to a higher expression. ( B ) Bright-field image of a SAN-RA (N = 3) preparation used for immunolabeling of Ca v 1.3. ( C ) Immunolabeling of Ca v 1.3 (red) of the preparation in ( B ). ( D ) Immunolabeling of Ca v 1.3 in the enlarged region corresponding to the white circle in ( C ). ( E ) Close-up view of Ca v 1.3-expressing cells (red), together with nuclear labeling of DNA (blue), in the field corresponding to the central square in panel ( D ). The yellow arrow indicates Ca v 1.3 immunoreactive cells with punctate staining. Abbreviations: RA, right atrium; SVC, superior vena cava ; IVC, inferior vena cava ; IAS, inter-atrial septum; CS, coronary sinus (cut-open view); CT, crista terminalis . The white dashed line roughly indicates the CT boundary.
    Figure Legend Snippet: Expression of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (green) and HCN4 (red) in n = 64 isolated SANC with distribution of SANC based on Ca v 1.3 or HCN4 fluorescence intensity. Low fluorescence intensity is related to a lower expression of the channels while high density is related to a higher expression. ( B ) Bright-field image of a SAN-RA (N = 3) preparation used for immunolabeling of Ca v 1.3. ( C ) Immunolabeling of Ca v 1.3 (red) of the preparation in ( B ). ( D ) Immunolabeling of Ca v 1.3 in the enlarged region corresponding to the white circle in ( C ). ( E ) Close-up view of Ca v 1.3-expressing cells (red), together with nuclear labeling of DNA (blue), in the field corresponding to the central square in panel ( D ). The yellow arrow indicates Ca v 1.3 immunoreactive cells with punctate staining. Abbreviations: RA, right atrium; SVC, superior vena cava ; IVC, inferior vena cava ; IAS, inter-atrial septum; CS, coronary sinus (cut-open view); CT, crista terminalis . The white dashed line roughly indicates the CT boundary.

    Techniques Used: Expressing, Immunolabeling, Isolation, Fluorescence, Labeling, Staining

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    Alomone Labs guinea pig anti hcn4 antibody
    Co-labeling of Ca v 1.3 and <t>HCN4</t> in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (red) and HCN4 (green) in the isolated cranial SAN-RA preparation. ( B ) Left panels: close-up views of the area circled in ( A ); right panels: enlargement with cells expressing Ca v 1.3 or HCN4, from boxed region in left panel. ( C ) Immunolabeling of regions co-expressing Ca v 1.3 and HCN4. The left panel shows the region in accordance with the posterior nodal extension (PNL). The yellow rectangle identifies the region considered for close-up views shown in the left panels. Left panels, from top to bottom, indicate corresponding Ca v 1.3 and HCN4 immunostaining. Yellow arrows indicate SANC co-expressing Ca v 1.3 and HCN4.
    Guinea Pig Anti Hcn4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti hcn4 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti hcn4 antibody - by Bioz Stars, 2022-08
    94/100 stars
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    Co-labeling of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (red) and HCN4 (green) in the isolated cranial SAN-RA preparation. ( B ) Left panels: close-up views of the area circled in ( A ); right panels: enlargement with cells expressing Ca v 1.3 or HCN4, from boxed region in left panel. ( C ) Immunolabeling of regions co-expressing Ca v 1.3 and HCN4. The left panel shows the region in accordance with the posterior nodal extension (PNL). The yellow rectangle identifies the region considered for close-up views shown in the left panels. Left panels, from top to bottom, indicate corresponding Ca v 1.3 and HCN4 immunostaining. Yellow arrows indicate SANC co-expressing Ca v 1.3 and HCN4.

    Journal: Cells

    Article Title: L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells

    doi: 10.3390/cells11071114

    Figure Lengend Snippet: Co-labeling of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (red) and HCN4 (green) in the isolated cranial SAN-RA preparation. ( B ) Left panels: close-up views of the area circled in ( A ); right panels: enlargement with cells expressing Ca v 1.3 or HCN4, from boxed region in left panel. ( C ) Immunolabeling of regions co-expressing Ca v 1.3 and HCN4. The left panel shows the region in accordance with the posterior nodal extension (PNL). The yellow rectangle identifies the region considered for close-up views shown in the left panels. Left panels, from top to bottom, indicate corresponding Ca v 1.3 and HCN4 immunostaining. Yellow arrows indicate SANC co-expressing Ca v 1.3 and HCN4.

    Article Snippet: SAN was incubated with anti-Cav1.3 (rabbit, 1:200, obtained from [ ] and anti-HCN4 (guinea pig, 1:200, Alomone labs, Cat. AGP-004) primary antibodies at 4 °C overnight.

    Techniques: Labeling, Immunolabeling, Isolation, Expressing, Immunostaining

    Expression of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (green) and HCN4 (red) in n = 64 isolated SANC with distribution of SANC based on Ca v 1.3 or HCN4 fluorescence intensity. Low fluorescence intensity is related to a lower expression of the channels while high density is related to a higher expression. ( B ) Bright-field image of a SAN-RA (N = 3) preparation used for immunolabeling of Ca v 1.3. ( C ) Immunolabeling of Ca v 1.3 (red) of the preparation in ( B ). ( D ) Immunolabeling of Ca v 1.3 in the enlarged region corresponding to the white circle in ( C ). ( E ) Close-up view of Ca v 1.3-expressing cells (red), together with nuclear labeling of DNA (blue), in the field corresponding to the central square in panel ( D ). The yellow arrow indicates Ca v 1.3 immunoreactive cells with punctate staining. Abbreviations: RA, right atrium; SVC, superior vena cava ; IVC, inferior vena cava ; IAS, inter-atrial septum; CS, coronary sinus (cut-open view); CT, crista terminalis . The white dashed line roughly indicates the CT boundary.

    Journal: Cells

    Article Title: L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells

    doi: 10.3390/cells11071114

    Figure Lengend Snippet: Expression of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (green) and HCN4 (red) in n = 64 isolated SANC with distribution of SANC based on Ca v 1.3 or HCN4 fluorescence intensity. Low fluorescence intensity is related to a lower expression of the channels while high density is related to a higher expression. ( B ) Bright-field image of a SAN-RA (N = 3) preparation used for immunolabeling of Ca v 1.3. ( C ) Immunolabeling of Ca v 1.3 (red) of the preparation in ( B ). ( D ) Immunolabeling of Ca v 1.3 in the enlarged region corresponding to the white circle in ( C ). ( E ) Close-up view of Ca v 1.3-expressing cells (red), together with nuclear labeling of DNA (blue), in the field corresponding to the central square in panel ( D ). The yellow arrow indicates Ca v 1.3 immunoreactive cells with punctate staining. Abbreviations: RA, right atrium; SVC, superior vena cava ; IVC, inferior vena cava ; IAS, inter-atrial septum; CS, coronary sinus (cut-open view); CT, crista terminalis . The white dashed line roughly indicates the CT boundary.

    Article Snippet: SAN was incubated with anti-Cav1.3 (rabbit, 1:200, obtained from [ ] and anti-HCN4 (guinea pig, 1:200, Alomone labs, Cat. AGP-004) primary antibodies at 4 °C overnight.

    Techniques: Expressing, Immunolabeling, Isolation, Fluorescence, Labeling, Staining