Rabbit Anti Sk1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti sk1/product/Alomone Labs
Average 93 stars, based on 1 article reviews
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1) Product Images from "Alterations of Electrophysiological Properties and Ion Channel Expression in Prefrontal Cortex of a Mouse Model of Schizophrenia"
Article Title: Alterations of Electrophysiological Properties and Ion Channel Expression in Prefrontal Cortex of a Mouse Model of Schizophrenia
Journal: Frontiers in Cellular Neuroscience
Figure Legend Snippet: MIA-SI increases the expression levels of Nav1.2 and SK3 channels. (A) Representative immunoblots of Nav1.2, Nav1.6 and tubulin, run on a same gel. Each band represents samples from one animal. (B,C) Average Nav1.2 and Nav1.6 protein levels in PFC in two experimental groups. (D) Averaged ratio of Nav1.2 to the sum of Nav1.2 and Nav1.6 was increased by MIA-SI. (E) Representative immunoblots of SK1, SK2, SK3, and tubulin run on a same gel. (F–H) Average SK1, SK2, and SK3 protein levels in PFC of experimental animals. For each protein, blots were cropped to retain the important bands at the same size of the same membrane. ∗ p
Techniques Used: Expressing, Western Blot
2) Product Images from "Role of the endothelial caveolae microdomain in shear stress–mediated coronary vasorelaxation"
Article Title: Role of the endothelial caveolae microdomain in shear stress–mediated coronary vasorelaxation
Journal: The Journal of Biological Chemistry
Figure Legend Snippet: Subcellular distribution of SK, IK, and TRP channels in freshly isolated nonpassaged BCECs is shown. A , the subcellular distribution of SK1, SK2, SK3, IK, TRPC4, and TRPV4 in freshly isolated BCECs was measured by density gradient fractionation, with fraction 1 being the lightest and fraction 12 the heaviest. Western blots against anti-SK1, anti-SK2, anti-SK3, anti-IK, anti-TRPV4, anti-TRPC4, and anti-Cav-1 antibodies show that SK1, SK3, and TRPV4 are detected in the low-buoyant density, caveolae-rich fractions, whereas SK2, IK, and TRPC4 are mainly found in the high-density non-lipid raft fractions and absent in the caveolae-rich fractions. B , immunoprecipitates of anti-Cav-1 antibody after incubation with the low-buoyant density fraction 5 were blotted against anti-SK1, anti-SK3, anti-IK, and anti-TRPV4 antibodies. Pulldowns using nonimmune IgG served as controls. C, immunoprecipitates of anti-TRPV4 or anti-SK3 antibodies from the low-buoyant density fraction 5 were blotted against anti-Cav-1 antibodies. Pulldown using nonimmune IgG served as controls. D, BCEC in situ proximity ligation assay (PLA). The fluorescent signal generated by labeled complementary oligonucleotide probes after a 100-min amplification reaction at 37 °C in BCECs treated with mouse anti-Cav-1 and rabbit anti-SK3 or rabbit anti-TRPV4 antibodies. The nuclei were counterstained with DAPI, and the PLA signals were visualized at 40× magnification under a Zeiss 510 Meta Confocal Laser Scanning Microscope equipped with DAPI/Texas Red filters and analyzed using Zeiss 510 software.
Techniques Used: Isolation, Fractionation, Western Blot, Incubation, In Situ, Proximity Ligation Assay, Generated, Labeling, Amplification, Laser-Scanning Microscopy, Software