anti girk3 kir3 3 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti girk3 kir3 3 antibody
    The effects of acute KET and CLZ administration on βarrestin1 ( A ), βarrestin2 ( B ), Grk2 ( C ), ERK1/2 ( D ), and <t>Girk3</t> ( E ) levels in the PFC. ( F ) The graphs show the correlation between mRNA expression and the βarrestin1 protein level. Representative images of each protein and βactin are shown in the upper panels. Bars represent the mean ± S.E.M. * p
    Anti Girk3 Kir3 3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti girk3 kir3 3 antibody/product/Alomone Labs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti girk3 kir3 3 antibody - by Bioz Stars, 2022-11
    93/100 stars

    Images

    1) Product Images from "Identification of Molecular Markers of Clozapine Action in Ketamine-Induced Cognitive Impairment: A GPCR Signaling PathwayFinder Study"

    Article Title: Identification of Molecular Markers of Clozapine Action in Ketamine-Induced Cognitive Impairment: A GPCR Signaling PathwayFinder Study

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms222212203

    The effects of acute KET and CLZ administration on βarrestin1 ( A ), βarrestin2 ( B ), Grk2 ( C ), ERK1/2 ( D ), and Girk3 ( E ) levels in the PFC. ( F ) The graphs show the correlation between mRNA expression and the βarrestin1 protein level. Representative images of each protein and βactin are shown in the upper panels. Bars represent the mean ± S.E.M. * p
    Figure Legend Snippet: The effects of acute KET and CLZ administration on βarrestin1 ( A ), βarrestin2 ( B ), Grk2 ( C ), ERK1/2 ( D ), and Girk3 ( E ) levels in the PFC. ( F ) The graphs show the correlation between mRNA expression and the βarrestin1 protein level. Representative images of each protein and βactin are shown in the upper panels. Bars represent the mean ± S.E.M. * p

    Techniques Used: Expressing

    The effects of sub-chronic KET and/or CLZ or HAL administration on βarrestin1 ( A ), βarrestin2 ( B ), Grk2 ( C ), ERK1/2 ( D ), and Girk3 ( E ) levels in the PFC, as well as representative membranes of each protein and βactin ( F ). Bars represent the mean ± S.E.M. * p
    Figure Legend Snippet: The effects of sub-chronic KET and/or CLZ or HAL administration on βarrestin1 ( A ), βarrestin2 ( B ), Grk2 ( C ), ERK1/2 ( D ), and Girk3 ( E ) levels in the PFC, as well as representative membranes of each protein and βactin ( F ). Bars represent the mean ± S.E.M. * p

    Techniques Used:

    2) Product Images from "Differential GABAB-Receptor-Mediated Effects in Perisomatic- and Dendrite-Targeting Parvalbumin Interneurons"

    Article Title: Differential GABAB-Receptor-Mediated Effects in Perisomatic- and Dendrite-Targeting Parvalbumin Interneurons

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.1186-12.2013

    Kir3 subunits are present on the plasma membrane of PV-immunoreactive interneurons. A–C , Electron micrographs of consecutive sections illustrating the localization of immunogold particles (arrows) for the Kir3.1 ( A ), Kir3.2 ( B ), and Kir3.3 ( C
    Figure Legend Snippet: Kir3 subunits are present on the plasma membrane of PV-immunoreactive interneurons. A–C , Electron micrographs of consecutive sections illustrating the localization of immunogold particles (arrows) for the Kir3.1 ( A ), Kir3.2 ( B ), and Kir3.3 ( C

    Techniques Used:

    3) Product Images from "Molecular and Cellular Diversity of Neuronal G-Protein-Gated Potassium Channels"

    Article Title: Molecular and Cellular Diversity of Neuronal G-Protein-Gated Potassium Channels

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.3484-05.2005

    GIRK subunit proteins are found in the mouse hippocampus. A , Representative immunoblots of hippocampal membrane protein samples from WT, GIRK1 KO (1), GIRK2 (2), GIRK3 (3), and GIRK2/GIRK3 (2/3) KO mice. Blots were probed with antibodies (Ab) for GIRK1, GIRK2, GIRK3, and GABA B(1a/b) . GABA B ), GIRK1 immunoreactivity was visualized as three bands, the lower molecular weight versions thought to represent core (c) and core-glycosylated (g) species. Reductions in the level of the heavily glycosylated (h) GIRK1 species were correlated with the absence of GIRK2 and GIRK3, respectively. The levels of GIRK2 and GIRK3 were also lower in samples from KO mice. B , Densitometric analysis of the impact of GIRK subunit ablation on residual GIRK protein levels in the hippocampus. Hippocampal protein samples were obtained from three separate and complete panels of WT and GIRK KO mice, and the levels of GIRK1 (heavily glycosylated form, h-GIRK1), GIRK2, GIRK3, and GABA B(1) (both isoforms) were determined. There was no effect of GIRK subunit ablation GABA B(1) receptor levels ( F (4,10) = 1.764; p = 0.213) (data not shown). * p
    Figure Legend Snippet: GIRK subunit proteins are found in the mouse hippocampus. A , Representative immunoblots of hippocampal membrane protein samples from WT, GIRK1 KO (1), GIRK2 (2), GIRK3 (3), and GIRK2/GIRK3 (2/3) KO mice. Blots were probed with antibodies (Ab) for GIRK1, GIRK2, GIRK3, and GABA B(1a/b) . GABA B ), GIRK1 immunoreactivity was visualized as three bands, the lower molecular weight versions thought to represent core (c) and core-glycosylated (g) species. Reductions in the level of the heavily glycosylated (h) GIRK1 species were correlated with the absence of GIRK2 and GIRK3, respectively. The levels of GIRK2 and GIRK3 were also lower in samples from KO mice. B , Densitometric analysis of the impact of GIRK subunit ablation on residual GIRK protein levels in the hippocampus. Hippocampal protein samples were obtained from three separate and complete panels of WT and GIRK KO mice, and the levels of GIRK1 (heavily glycosylated form, h-GIRK1), GIRK2, GIRK3, and GABA B(1) (both isoforms) were determined. There was no effect of GIRK subunit ablation GABA B(1) receptor levels ( F (4,10) = 1.764; p = 0.213) (data not shown). * p

    Techniques Used: Western Blot, Mouse Assay, Molecular Weight

    Subcellular distributions of GIRK subunits in the hippocampus. Electron micrographs show immunolabeling for GIRK1 and GIRK2 in the stratum radiatum of the CA1 area of WT and GIRK KO mice. den, Dendritic shaft; b, bouton; s, dendritic spine. A , B , GIRK1 immunoparticles were observed primarily along the extrasynaptic plasma membrane (arrows) of dendritic spines of CA1 neurons from WT mice, although perisynaptic labeling (crossed arrow) was also frequently observed. GIRK1 labeling was also found associated with ER cisterna of dendritic shafts and with the spine apparatus (double arrowheads) of spines. C , GIRK1 labeling was never detected within the postsynaptic specialization, as demonstrated with postembedding techniques. D , E , GIRK1 immunoparticles at presynaptic sites were localized to the extrasynaptic plasma membrane (arrowheads) and occasionally to the presynaptic membrane specialization of axon terminals establishing putative excitatory synapses on spines, as confirmed in double-labeling experiments with VGluT1. F-H , GIRK2 immunoparticles were found along the extrasynaptic plasma membrane (arrows) of dendritic shafts of CA1 cells from WT mice, mainly associated with dendritic spines. Both perisynaptic ( G , crossed arrows) and synaptic ( H , double arrow) labeling at asymmetrical synapses was detected, the latter confirmed using postembedding techniques ( I ). J , K , GIRK2 immunoparticles were detected at presynaptic sites (arrowheads), mainly in the extra synaptic plasma membrane, and occasionally in the presynaptic membrane specialization of axon terminals establishing putative excitatory synapses on spines, as confirmed using double-labeling experiments with VGluT1. Note also the GIRK2 immunoreactivity associated with ER cisterna of dendritic shafts and the spine apparatus of dendritic spines ( J , double arrowheads). L , Distribution of GIRK1 and GIRK2 on dendritic spines of CA1 neurons. Data are displayed as percentage frequency of particles in 60-nm-wide bins, starting at the edge of the postsynaptic specialization. M-T , Electron micrographs showing GIRK1 immunoreactivity in the stratum radiatum of the CA1 area in sections taken from GIRK2 KO ( M-P ), GIRK3 KO ( Q , R ), GIRK2/GIRK3 KO ( S ), and GIRK1 KO ( T ) mice. Note that GIRK1 immunoreactivity was still observed along the extrasynaptic plasma membrane (arrows) of dendritic spines and shafts of CA1 neurons from GIRK2 KO and GIRK3 KO mice. Immunoparticles for GIRK1 were also observed at perisynaptic (data not shown) positions and at presynaptic sites along the extrasynaptic plasma membrane (arrowheads) of axon terminals establishing putative excitatory synapses on dendritic spines. A higher proportion of immunoparticles for GIRK1 was detected in the ER cisterna of dendritic shafts and the spine apparatus (double arrowhead). Immunoreactivity for GIRK1 was primarily restricted to the ER of CA1 cells in the hippocampus of GIRK2/GIRK3 KO mice and was absent in sections from a GIRK1 KO mouse. Scale bars, 0.2 μm.
    Figure Legend Snippet: Subcellular distributions of GIRK subunits in the hippocampus. Electron micrographs show immunolabeling for GIRK1 and GIRK2 in the stratum radiatum of the CA1 area of WT and GIRK KO mice. den, Dendritic shaft; b, bouton; s, dendritic spine. A , B , GIRK1 immunoparticles were observed primarily along the extrasynaptic plasma membrane (arrows) of dendritic spines of CA1 neurons from WT mice, although perisynaptic labeling (crossed arrow) was also frequently observed. GIRK1 labeling was also found associated with ER cisterna of dendritic shafts and with the spine apparatus (double arrowheads) of spines. C , GIRK1 labeling was never detected within the postsynaptic specialization, as demonstrated with postembedding techniques. D , E , GIRK1 immunoparticles at presynaptic sites were localized to the extrasynaptic plasma membrane (arrowheads) and occasionally to the presynaptic membrane specialization of axon terminals establishing putative excitatory synapses on spines, as confirmed in double-labeling experiments with VGluT1. F-H , GIRK2 immunoparticles were found along the extrasynaptic plasma membrane (arrows) of dendritic shafts of CA1 cells from WT mice, mainly associated with dendritic spines. Both perisynaptic ( G , crossed arrows) and synaptic ( H , double arrow) labeling at asymmetrical synapses was detected, the latter confirmed using postembedding techniques ( I ). J , K , GIRK2 immunoparticles were detected at presynaptic sites (arrowheads), mainly in the extra synaptic plasma membrane, and occasionally in the presynaptic membrane specialization of axon terminals establishing putative excitatory synapses on spines, as confirmed using double-labeling experiments with VGluT1. Note also the GIRK2 immunoreactivity associated with ER cisterna of dendritic shafts and the spine apparatus of dendritic spines ( J , double arrowheads). L , Distribution of GIRK1 and GIRK2 on dendritic spines of CA1 neurons. Data are displayed as percentage frequency of particles in 60-nm-wide bins, starting at the edge of the postsynaptic specialization. M-T , Electron micrographs showing GIRK1 immunoreactivity in the stratum radiatum of the CA1 area in sections taken from GIRK2 KO ( M-P ), GIRK3 KO ( Q , R ), GIRK2/GIRK3 KO ( S ), and GIRK1 KO ( T ) mice. Note that GIRK1 immunoreactivity was still observed along the extrasynaptic plasma membrane (arrows) of dendritic spines and shafts of CA1 neurons from GIRK2 KO and GIRK3 KO mice. Immunoparticles for GIRK1 were also observed at perisynaptic (data not shown) positions and at presynaptic sites along the extrasynaptic plasma membrane (arrowheads) of axon terminals establishing putative excitatory synapses on dendritic spines. A higher proportion of immunoparticles for GIRK1 was detected in the ER cisterna of dendritic shafts and the spine apparatus (double arrowhead). Immunoreactivity for GIRK1 was primarily restricted to the ER of CA1 cells in the hippocampus of GIRK2/GIRK3 KO mice and was absent in sections from a GIRK1 KO mouse. Scale bars, 0.2 μm.

    Techniques Used: Immunolabeling, Mouse Assay, Labeling

    GIRK subunit mRNA distribution in the hippocampus and substantia nigra. GIRK mRNA distributions were evaluated by in situ hybridization in sections from WT and GIRKKO mice. In sections from WT mice, mRNAs for GIRK1 ( A ), GIRK2 ( B ), and GIRK3 ( C ) were clearly evident in CA1 and CA3 pyramidal neurons, as well as granule cells of the dentate gyrus (DG). There was no specific staining for GIRK mRNAs in sections from the appropriate GIRK KO mouse (data not shown). Images are representative of data obtained from three different WT and GIRK KO panels. The WT mRNA distributions of GIRK1 ( D ), GIRK2 ( E ), and GIRK3 ( F ) were also examined in the SN. Scale bars: A-C , 500 μm; D-F , 100 μm.
    Figure Legend Snippet: GIRK subunit mRNA distribution in the hippocampus and substantia nigra. GIRK mRNA distributions were evaluated by in situ hybridization in sections from WT and GIRKKO mice. In sections from WT mice, mRNAs for GIRK1 ( A ), GIRK2 ( B ), and GIRK3 ( C ) were clearly evident in CA1 and CA3 pyramidal neurons, as well as granule cells of the dentate gyrus (DG). There was no specific staining for GIRK mRNAs in sections from the appropriate GIRK KO mouse (data not shown). Images are representative of data obtained from three different WT and GIRK KO panels. The WT mRNA distributions of GIRK1 ( D ), GIRK2 ( E ), and GIRK3 ( F ) were also examined in the SN. Scale bars: A-C , 500 μm; D-F , 100 μm.

    Techniques Used: In Situ Hybridization, Mouse Assay, Staining

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    Alomone Labs anti girk3 kir3 3 antibody
    The effects of acute KET and CLZ administration on βarrestin1 ( A ), βarrestin2 ( B ), Grk2 ( C ), ERK1/2 ( D ), and <t>Girk3</t> ( E ) levels in the PFC. ( F ) The graphs show the correlation between mRNA expression and the βarrestin1 protein level. Representative images of each protein and βactin are shown in the upper panels. Bars represent the mean ± S.E.M. * p
    Anti Girk3 Kir3 3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti girk3 kir3 3 antibody/product/Alomone Labs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti girk3 kir3 3 antibody - by Bioz Stars, 2022-11
    93/100 stars
      Buy from Supplier

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    The effects of acute KET and CLZ administration on βarrestin1 ( A ), βarrestin2 ( B ), Grk2 ( C ), ERK1/2 ( D ), and Girk3 ( E ) levels in the PFC. ( F ) The graphs show the correlation between mRNA expression and the βarrestin1 protein level. Representative images of each protein and βactin are shown in the upper panels. Bars represent the mean ± S.E.M. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Identification of Molecular Markers of Clozapine Action in Ketamine-Induced Cognitive Impairment: A GPCR Signaling PathwayFinder Study

    doi: 10.3390/ijms222212203

    Figure Lengend Snippet: The effects of acute KET and CLZ administration on βarrestin1 ( A ), βarrestin2 ( B ), Grk2 ( C ), ERK1/2 ( D ), and Girk3 ( E ) levels in the PFC. ( F ) The graphs show the correlation between mRNA expression and the βarrestin1 protein level. Representative images of each protein and βactin are shown in the upper panels. Bars represent the mean ± S.E.M. * p

    Article Snippet: Primary antibodies for βarrestin1 (ab31868, Abcam, Cambridge, UK), βarrestin2 (ab31294, Abcam, Cambridge, UK), ERK1/2 (sc-514302, Santa Cruz, CA, USA), Grk2 (sc-562, Santa Cruz, CA, USA), and GIRK 3 (APC-038, Alomone Labs, Jerusalem, Israel) were used at a concentration of 1:200.

    Techniques: Expressing

    The effects of sub-chronic KET and/or CLZ or HAL administration on βarrestin1 ( A ), βarrestin2 ( B ), Grk2 ( C ), ERK1/2 ( D ), and Girk3 ( E ) levels in the PFC, as well as representative membranes of each protein and βactin ( F ). Bars represent the mean ± S.E.M. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Identification of Molecular Markers of Clozapine Action in Ketamine-Induced Cognitive Impairment: A GPCR Signaling PathwayFinder Study

    doi: 10.3390/ijms222212203

    Figure Lengend Snippet: The effects of sub-chronic KET and/or CLZ or HAL administration on βarrestin1 ( A ), βarrestin2 ( B ), Grk2 ( C ), ERK1/2 ( D ), and Girk3 ( E ) levels in the PFC, as well as representative membranes of each protein and βactin ( F ). Bars represent the mean ± S.E.M. * p

    Article Snippet: Primary antibodies for βarrestin1 (ab31868, Abcam, Cambridge, UK), βarrestin2 (ab31294, Abcam, Cambridge, UK), ERK1/2 (sc-514302, Santa Cruz, CA, USA), Grk2 (sc-562, Santa Cruz, CA, USA), and GIRK 3 (APC-038, Alomone Labs, Jerusalem, Israel) were used at a concentration of 1:200.

    Techniques:

    Kir3 subunits are present on the plasma membrane of PV-immunoreactive interneurons. A–C , Electron micrographs of consecutive sections illustrating the localization of immunogold particles (arrows) for the Kir3.1 ( A ), Kir3.2 ( B ), and Kir3.3 ( C

    Journal: The Journal of Neuroscience

    Article Title: Differential GABAB-Receptor-Mediated Effects in Perisomatic- and Dendrite-Targeting Parvalbumin Interneurons

    doi: 10.1523/JNEUROSCI.1186-12.2013

    Figure Lengend Snippet: Kir3 subunits are present on the plasma membrane of PV-immunoreactive interneurons. A–C , Electron micrographs of consecutive sections illustrating the localization of immunogold particles (arrows) for the Kir3.1 ( A ), Kir3.2 ( B ), and Kir3.3 ( C

    Article Snippet: Affinity-purified rabbit polyclonal antibodies were used to detect Kir3.1 and Kir3.3 subunits ( ); a commercially available rabbit polyclonal antibody was used against Kir3.2 (Alomone Labs).

    Techniques: