kcnj10  (Alomone Labs)


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    Alomone Labs kcnj10
    Kcnj10, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kcnj10/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kcnj10 - by Bioz Stars, 2022-11
    95/100 stars

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    Alomone Labs anti kir4 1 kcnj10 antibody
    <t>Kir4.1,</t> Kir5.1, and pS373-SPAK levels are increased in tubule suspensions isolated from Nedd4-2 KO mice. (A+B) Semi-quantitative assessment of Kir4.1, Kir5.1, and pS373-SPAK levels in tubules isolated from control and Nedd4-2 KO mice treated with 0.5 mM, 3.5 mM, or 8.0 mM K + for 24 h. (A) Data are means ± S.E.M and normalized to control tubules treated with 3.5 mM K + . Difference between control and KO tubules treated with either 0.5 mM, 3.5 mM, or 8.0 mM K + is assessed by 2way ANOVA followed by Tukey’s multiple comparisons test (* p
    Anti Kir4 1 Kcnj10 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kir4 1 kcnj10 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kir4 1 kcnj10 antibody - by Bioz Stars, 2022-11
    95/100 stars
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    Kir4.1, Kir5.1, and pS373-SPAK levels are increased in tubule suspensions isolated from Nedd4-2 KO mice. (A+B) Semi-quantitative assessment of Kir4.1, Kir5.1, and pS373-SPAK levels in tubules isolated from control and Nedd4-2 KO mice treated with 0.5 mM, 3.5 mM, or 8.0 mM K + for 24 h. (A) Data are means ± S.E.M and normalized to control tubules treated with 3.5 mM K + . Difference between control and KO tubules treated with either 0.5 mM, 3.5 mM, or 8.0 mM K + is assessed by 2way ANOVA followed by Tukey’s multiple comparisons test (* p

    Journal: Frontiers in Physiology

    Article Title: The E3 ubiquitin-protein ligase Nedd4-2 regulates the sodium chloride cotransporter NCC but is not required for a potassium-induced reduction of NCC expression

    doi: 10.3389/fphys.2022.971251

    Figure Lengend Snippet: Kir4.1, Kir5.1, and pS373-SPAK levels are increased in tubule suspensions isolated from Nedd4-2 KO mice. (A+B) Semi-quantitative assessment of Kir4.1, Kir5.1, and pS373-SPAK levels in tubules isolated from control and Nedd4-2 KO mice treated with 0.5 mM, 3.5 mM, or 8.0 mM K + for 24 h. (A) Data are means ± S.E.M and normalized to control tubules treated with 3.5 mM K + . Difference between control and KO tubules treated with either 0.5 mM, 3.5 mM, or 8.0 mM K + is assessed by 2way ANOVA followed by Tukey’s multiple comparisons test (* p

    Article Snippet: Immunoblotting was performed using standard methods and the following primary antibodies: rabbit polyclonal antibodies against NCC (SPC-402D, StressMarq), phosphorylated Threonine-58 NCC (pT58-NCC) , Nedd4-2 , phosphorylated Serine373 SPAK/Serine325 OSR1 (pS373-SPAK) (07–2273, Sigma), SPAK/OSR1 , Kir4.1 (APC-035, Alomone), Kir5.1 (LS-C177333, LS Bio), Proteasome 20S (ab3325, Abcam) and Actin (A2066, Sigma) plus a mouse monoclonal NCC antibody ( ) .

    Techniques: Isolation, Mouse Assay

    AAV8BP2-GFP and AAV8-GFP transduced the intermediate cells in the stria vascularis (A) Confocal images of the cochlear lateral wall at the level of the intermediate cell layer are shown (single optical section). Anti-KCNJ10 antibody was used as an intermediate cell marker. AAV8BP2-GFP (n = 9) and AAV8-GFP (n = 9) transduced the intermediate cells in the stria vascularis, but AAV2.7m8-GFP (n = 4), Anc80L65-GFP (n = 5), and AAV2-GFP (n = 4) showed minimal intermediate cell transduction. All images were taken at ∼P30. Surgeries were performed in P0–5 animals and the average surgery age was P1.9. The scale bar represents 50 μm for the lower-magnification images, and 10 μm for the magnified images. White dashed squares indicate areas where magnified images are taken. (B) Quantification of the intermediate cell transduction efficiency with various AAV serotypes. Both individual (open circles) and average results are presented. ∗p

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: AAV8BP2 and AAV8 transduce the mammalian cochlear lateral wall and endolymphatic sac with high efficiency

    doi: 10.1016/j.omtm.2022.07.013

    Figure Lengend Snippet: AAV8BP2-GFP and AAV8-GFP transduced the intermediate cells in the stria vascularis (A) Confocal images of the cochlear lateral wall at the level of the intermediate cell layer are shown (single optical section). Anti-KCNJ10 antibody was used as an intermediate cell marker. AAV8BP2-GFP (n = 9) and AAV8-GFP (n = 9) transduced the intermediate cells in the stria vascularis, but AAV2.7m8-GFP (n = 4), Anc80L65-GFP (n = 5), and AAV2-GFP (n = 4) showed minimal intermediate cell transduction. All images were taken at ∼P30. Surgeries were performed in P0–5 animals and the average surgery age was P1.9. The scale bar represents 50 μm for the lower-magnification images, and 10 μm for the magnified images. White dashed squares indicate areas where magnified images are taken. (B) Quantification of the intermediate cell transduction efficiency with various AAV serotypes. Both individual (open circles) and average results are presented. ∗p

    Article Snippet: For the primary antibodies, SLC12A2 antibodies were used to label marginal cells (1:100, Cat# sc21545; Santa Cruz Biotech, Dallas, TX), and KCNJ10 antibodies for intermediate cells (1:100, Cat# APC-035; Alomone Labs, Jerusalem, Israel).

    Techniques: Marker, Transduction