Journal: Journal of Cellular and Molecular Medicine
Article Title: Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia. Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia
Figure Lengend Snippet: 3D‐reconstructed confocal laser images of formalin‐fixed paraffin‐embedded canine spinal ganglia (A‐D): Double labelling with glutamine synthetase (GS; green; A, B) or inwardly rectifying potassium channel Kir 4.1 (green; C, D), respectively, and the neuronal marker NeuN (magenta). Nuclei are counterstained with bisbenzimide (blue). The zoomed in pictures (B, D) show that GS‐, respectively, Kir4.1‐positive satellite glial cells (SGCs) tightly envelop NeuN‐positive neurons. For GS/NeuN staining (A‐B), 32 z‐stack frames (5.2 µm total size; approx. 0.16 µm steps) and for Kir4.1/NeuN staining (C‐D), 31 z‐stack frames (5.0 µm total size; approx. 0.16 µm steps) were collected. Scale bars: 20 µm. A movie of 3D confocal reconstructions is provided in Video [Link] , [Link] and Video [Link] , [Link]
Article Snippet: For double labelling of the directly labelled Kir 4.1 (APC‐035‐AG, Alomone laboratories Ltd,) with NG2 (AB5320, Sigma‐Aldrich, Merck KGaA) or periaxin (HPA001868‐100UL, Sigma‐Aldrich, Merck KGaA), non‐labelled antibodies were initially incubated for 24 hours.
Techniques: Formalin-fixed Paraffin-Embedded, Marker, Staining