kir 4 1  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Anti Kir4 1 KCNJ10 ATTO Fluor 488 Antibody
    Description:
    Anti Kir4 1 KCNJ10 Antibody APC 035 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunoprecipitation immunohistochemistry and immunocytochemistry applications It has been designed to recognize Kir4 1 potassium channel from rat mouse and human samples nAnti Kir4 1 KCNJ10 ATTO Fluor 488 Antibody APC 035 AG is directly labeled with an ATTO 488 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 488 label is analogous to the well known dye fluorescein isothiocyanate FITC and can be used with filters typically used to detect FITC Anti Kir4 1 KCNJ10 ATTO Fluor 488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers
    Catalog Number:
    APC-035-AG
    Price:
    686.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal ATTO 488 (Green) Conjugated Primary Antibody
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Alomone Labs kir 4 1
    Anti Kir4 1 KCNJ10 ATTO Fluor 488 Antibody
    Anti Kir4 1 KCNJ10 Antibody APC 035 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunoprecipitation immunohistochemistry and immunocytochemistry applications It has been designed to recognize Kir4 1 potassium channel from rat mouse and human samples nAnti Kir4 1 KCNJ10 ATTO Fluor 488 Antibody APC 035 AG is directly labeled with an ATTO 488 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 488 label is analogous to the well known dye fluorescein isothiocyanate FITC and can be used with filters typically used to detect FITC Anti Kir4 1 KCNJ10 ATTO Fluor 488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers
    https://www.bioz.com/result/kir 4 1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kir 4 1 - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia. Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia"

    Article Title: Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia. Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.16701

    3D‐reconstructed confocal laser images of formalin‐fixed paraffin‐embedded canine spinal ganglia (A‐D): Double labelling with glutamine synthetase (GS; green; A, B) or inwardly rectifying potassium channel Kir 4.1 (green; C, D), respectively, and the neuronal marker NeuN (magenta). Nuclei are counterstained with bisbenzimide (blue). The zoomed in pictures (B, D) show that GS‐, respectively, Kir4.1‐positive satellite glial cells (SGCs) tightly envelop NeuN‐positive neurons. For GS/NeuN staining (A‐B), 32 z‐stack frames (5.2 µm total size; approx. 0.16 µm steps) and for Kir4.1/NeuN staining (C‐D), 31 z‐stack frames (5.0 µm total size; approx. 0.16 µm steps) were collected. Scale bars: 20 µm. A movie of 3D confocal reconstructions is provided in Video   [Link] ,   [Link]  and Video   [Link] ,   [Link]
    Figure Legend Snippet: 3D‐reconstructed confocal laser images of formalin‐fixed paraffin‐embedded canine spinal ganglia (A‐D): Double labelling with glutamine synthetase (GS; green; A, B) or inwardly rectifying potassium channel Kir 4.1 (green; C, D), respectively, and the neuronal marker NeuN (magenta). Nuclei are counterstained with bisbenzimide (blue). The zoomed in pictures (B, D) show that GS‐, respectively, Kir4.1‐positive satellite glial cells (SGCs) tightly envelop NeuN‐positive neurons. For GS/NeuN staining (A‐B), 32 z‐stack frames (5.2 µm total size; approx. 0.16 µm steps) and for Kir4.1/NeuN staining (C‐D), 31 z‐stack frames (5.0 µm total size; approx. 0.16 µm steps) were collected. Scale bars: 20 µm. A movie of 3D confocal reconstructions is provided in Video [Link] , [Link] and Video [Link] , [Link]

    Techniques Used: Formalin-fixed Paraffin-Embedded, Marker, Staining

    3D‐reconstructed confocal laser images of formalin‐fixed paraffin‐embedded murine spinal ganglia (A‐D): Double labelling with glutamine synthetase (GS; green; A, B) or inwardly rectifying potassium channel Kir 4.1 (green; C, D), respectively, and the neuronal marker NeuN (magenta). Nuclei are counterstained with Bisbenzimide (blue). GS‐ / Kir4.1‐positive satellite glial cells (SGCs) form a tight sheath around the neuronal bodies. The zoomed in images (B, D) clearly illustrate the close contact between SGCs and neurons. For GS/NeuN staining (A‐B), 39 z‐stack frames (6.4 µm total size; approx. 0.16 µm steps) and for Kir4.1/NeuN staining (C‐D), 39 z‐stack frames (4.7 µm total size; approx. 0.12 µm steps) were obtained. Scale bars: 20 µm (A, B, C); 10 µm (D). A movie of 3D confocal reconstructions is provided in Video   [Link] ,   [Link]  and Video   [Link] ,   [Link]
    Figure Legend Snippet: 3D‐reconstructed confocal laser images of formalin‐fixed paraffin‐embedded murine spinal ganglia (A‐D): Double labelling with glutamine synthetase (GS; green; A, B) or inwardly rectifying potassium channel Kir 4.1 (green; C, D), respectively, and the neuronal marker NeuN (magenta). Nuclei are counterstained with Bisbenzimide (blue). GS‐ / Kir4.1‐positive satellite glial cells (SGCs) form a tight sheath around the neuronal bodies. The zoomed in images (B, D) clearly illustrate the close contact between SGCs and neurons. For GS/NeuN staining (A‐B), 39 z‐stack frames (6.4 µm total size; approx. 0.16 µm steps) and for Kir4.1/NeuN staining (C‐D), 39 z‐stack frames (4.7 µm total size; approx. 0.12 µm steps) were obtained. Scale bars: 20 µm (A, B, C); 10 µm (D). A movie of 3D confocal reconstructions is provided in Video [Link] , [Link] and Video [Link] , [Link]

    Techniques Used: Formalin-fixed Paraffin-Embedded, Marker, Staining

    Related Articles

    Immunohistochemistry-IF:

    Article Title: Loss of astrocyte polarization upon transient focal brain ischemia as a possible mechanism to counteract early edema formation.
    Article Snippet: .. Brain edema is the main cause of death from brain infarction.. The polarized expression of the water channel protein aquaporin-4 (AQP4) on astroglial endfeet surrounding brain microvessels suggests a role in brain water balance. ..

    Immunohistochemistry:

    Article Title: Loss of astrocyte polarization upon transient focal brain ischemia as a possible mechanism to counteract early edema formation.
    Article Snippet: .. Brain edema is the main cause of death from brain infarction.. The polarized expression of the water channel protein aquaporin-4 (AQP4) on astroglial endfeet surrounding brain microvessels suggests a role in brain water balance. ..

    Purification:

    Article Title: Loss of astrocyte polarization upon transient focal brain ischemia as a possible mechanism to counteract early edema formation.
    Article Snippet: .. Brain edema is the main cause of death from brain infarction.. The polarized expression of the water channel protein aquaporin-4 (AQP4) on astroglial endfeet surrounding brain microvessels suggests a role in brain water balance. ..

    Incubation:

    Article Title: Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia. Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia
    Article Snippet: .. For double labelling of the directly labelled Kir 4.1 (APC‐035‐AG, Alomone laboratories Ltd,) with NG2 (AB5320, Sigma‐Aldrich, Merck KGaA) or periaxin (HPA001868‐100UL, Sigma‐Aldrich, Merck KGaA), non‐labelled antibodies were initially incubated for 24 hours. ..

    Staining:

    Article Title: Loss of astrocyte polarization upon transient focal brain ischemia as a possible mechanism to counteract early edema formation.
    Article Snippet: .. Brain edema is the main cause of death from brain infarction.. The polarized expression of the water channel protein aquaporin-4 (AQP4) on astroglial endfeet surrounding brain microvessels suggests a role in brain water balance. ..

    Blocking Assay:

    Article Title: Loss of astrocyte polarization upon transient focal brain ischemia as a possible mechanism to counteract early edema formation.
    Article Snippet: .. Brain edema is the main cause of death from brain infarction.. The polarized expression of the water channel protein aquaporin-4 (AQP4) on astroglial endfeet surrounding brain microvessels suggests a role in brain water balance. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs kir 4 1
    3D‐reconstructed confocal laser images of formalin‐fixed paraffin‐embedded canine spinal ganglia (A‐D): Double labelling with glutamine synthetase (GS; green; A, B) or inwardly rectifying potassium channel Kir 4.1 (green; C, D), respectively, and the neuronal marker NeuN (magenta). Nuclei are counterstained with bisbenzimide (blue). The zoomed in pictures (B, D) show that GS‐, respectively, Kir4.1‐positive satellite glial cells (SGCs) tightly envelop NeuN‐positive neurons. For GS/NeuN staining (A‐B), 32 z‐stack frames (5.2 µm total size; approx. 0.16 µm steps) and for Kir4.1/NeuN staining (C‐D), 31 z‐stack frames (5.0 µm total size; approx. 0.16 µm steps) were collected. Scale bars: 20 µm. A movie of 3D confocal reconstructions is provided in Video   [Link] ,   [Link]  and Video   [Link] ,   [Link]
    Kir 4 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kir 4 1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kir 4 1 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    3D‐reconstructed confocal laser images of formalin‐fixed paraffin‐embedded canine spinal ganglia (A‐D): Double labelling with glutamine synthetase (GS; green; A, B) or inwardly rectifying potassium channel Kir 4.1 (green; C, D), respectively, and the neuronal marker NeuN (magenta). Nuclei are counterstained with bisbenzimide (blue). The zoomed in pictures (B, D) show that GS‐, respectively, Kir4.1‐positive satellite glial cells (SGCs) tightly envelop NeuN‐positive neurons. For GS/NeuN staining (A‐B), 32 z‐stack frames (5.2 µm total size; approx. 0.16 µm steps) and for Kir4.1/NeuN staining (C‐D), 31 z‐stack frames (5.0 µm total size; approx. 0.16 µm steps) were collected. Scale bars: 20 µm. A movie of 3D confocal reconstructions is provided in Video   [Link] ,   [Link]  and Video   [Link] ,   [Link]

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia. Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia

    doi: 10.1111/jcmm.16701

    Figure Lengend Snippet: 3D‐reconstructed confocal laser images of formalin‐fixed paraffin‐embedded canine spinal ganglia (A‐D): Double labelling with glutamine synthetase (GS; green; A, B) or inwardly rectifying potassium channel Kir 4.1 (green; C, D), respectively, and the neuronal marker NeuN (magenta). Nuclei are counterstained with bisbenzimide (blue). The zoomed in pictures (B, D) show that GS‐, respectively, Kir4.1‐positive satellite glial cells (SGCs) tightly envelop NeuN‐positive neurons. For GS/NeuN staining (A‐B), 32 z‐stack frames (5.2 µm total size; approx. 0.16 µm steps) and for Kir4.1/NeuN staining (C‐D), 31 z‐stack frames (5.0 µm total size; approx. 0.16 µm steps) were collected. Scale bars: 20 µm. A movie of 3D confocal reconstructions is provided in Video [Link] , [Link] and Video [Link] , [Link]

    Article Snippet: For double labelling of the directly labelled Kir 4.1 (APC‐035‐AG, Alomone laboratories Ltd,) with NG2 (AB5320, Sigma‐Aldrich, Merck KGaA) or periaxin (HPA001868‐100UL, Sigma‐Aldrich, Merck KGaA), non‐labelled antibodies were initially incubated for 24 hours.

    Techniques: Formalin-fixed Paraffin-Embedded, Marker, Staining

    3D‐reconstructed confocal laser images of formalin‐fixed paraffin‐embedded murine spinal ganglia (A‐D): Double labelling with glutamine synthetase (GS; green; A, B) or inwardly rectifying potassium channel Kir 4.1 (green; C, D), respectively, and the neuronal marker NeuN (magenta). Nuclei are counterstained with Bisbenzimide (blue). GS‐ / Kir4.1‐positive satellite glial cells (SGCs) form a tight sheath around the neuronal bodies. The zoomed in images (B, D) clearly illustrate the close contact between SGCs and neurons. For GS/NeuN staining (A‐B), 39 z‐stack frames (6.4 µm total size; approx. 0.16 µm steps) and for Kir4.1/NeuN staining (C‐D), 39 z‐stack frames (4.7 µm total size; approx. 0.12 µm steps) were obtained. Scale bars: 20 µm (A, B, C); 10 µm (D). A movie of 3D confocal reconstructions is provided in Video   [Link] ,   [Link]  and Video   [Link] ,   [Link]

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia. Phenotypical peculiarities and species‐specific differences of canine and murine satellite glial cells of spinal ganglia

    doi: 10.1111/jcmm.16701

    Figure Lengend Snippet: 3D‐reconstructed confocal laser images of formalin‐fixed paraffin‐embedded murine spinal ganglia (A‐D): Double labelling with glutamine synthetase (GS; green; A, B) or inwardly rectifying potassium channel Kir 4.1 (green; C, D), respectively, and the neuronal marker NeuN (magenta). Nuclei are counterstained with Bisbenzimide (blue). GS‐ / Kir4.1‐positive satellite glial cells (SGCs) form a tight sheath around the neuronal bodies. The zoomed in images (B, D) clearly illustrate the close contact between SGCs and neurons. For GS/NeuN staining (A‐B), 39 z‐stack frames (6.4 µm total size; approx. 0.16 µm steps) and for Kir4.1/NeuN staining (C‐D), 39 z‐stack frames (4.7 µm total size; approx. 0.12 µm steps) were obtained. Scale bars: 20 µm (A, B, C); 10 µm (D). A movie of 3D confocal reconstructions is provided in Video [Link] , [Link] and Video [Link] , [Link]

    Article Snippet: For double labelling of the directly labelled Kir 4.1 (APC‐035‐AG, Alomone laboratories Ltd,) with NG2 (AB5320, Sigma‐Aldrich, Merck KGaA) or periaxin (HPA001868‐100UL, Sigma‐Aldrich, Merck KGaA), non‐labelled antibodies were initially incubated for 24 hours.

    Techniques: Formalin-fixed Paraffin-Embedded, Marker, Staining