hcn2  (Alomone Labs)


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    Structured Review

    Alomone Labs hcn2
    HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layer 5-6 of mPFC. A-C. Microscopic confocal images showing HCN1-ir ( A ), <t>HCN2-ir</t> ( B ), and HCN4-ir ( C ) locate in PV-ir interneuron in layer 5-6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bar, 20 μm D. High-magnification confocal microscopy images showing that HCN1-ir localize in the soma ( d1 ) and along neurite ( d2-d3 ) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite ( d1 ) is digital magnified for better view of neurite in ( d2 ) and ( d3 ), respectively. Scale bars, 20 μm in ( d1 ) and 1 μm in ( d2 ) and ( d3 ). E. High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digital magnified for better view of neurite in (e2). Scale bars, 20 μm in (e1) and 1 μm in (e2).
    Hcn2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex"

    Article Title: Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex

    Journal: bioRxiv

    doi: 10.1101/2021.05.23.445332

    HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layer 5-6 of mPFC. A-C. Microscopic confocal images showing HCN1-ir ( A ), HCN2-ir ( B ), and HCN4-ir ( C ) locate in PV-ir interneuron in layer 5-6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bar, 20 μm D. High-magnification confocal microscopy images showing that HCN1-ir localize in the soma ( d1 ) and along neurite ( d2-d3 ) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite ( d1 ) is digital magnified for better view of neurite in ( d2 ) and ( d3 ), respectively. Scale bars, 20 μm in ( d1 ) and 1 μm in ( d2 ) and ( d3 ). E. High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digital magnified for better view of neurite in (e2). Scale bars, 20 μm in (e1) and 1 μm in (e2).
    Figure Legend Snippet: HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layer 5-6 of mPFC. A-C. Microscopic confocal images showing HCN1-ir ( A ), HCN2-ir ( B ), and HCN4-ir ( C ) locate in PV-ir interneuron in layer 5-6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bar, 20 μm D. High-magnification confocal microscopy images showing that HCN1-ir localize in the soma ( d1 ) and along neurite ( d2-d3 ) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite ( d1 ) is digital magnified for better view of neurite in ( d2 ) and ( d3 ), respectively. Scale bars, 20 μm in ( d1 ) and 1 μm in ( d2 ) and ( d3 ). E. High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digital magnified for better view of neurite in (e2). Scale bars, 20 μm in (e1) and 1 μm in (e2).

    Techniques Used: Expressing, Staining, Labeling, Confocal Microscopy

    HCN channels are present on GABAergic terminals in the mPFC. A. Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrating the cells in layer 5-6 of the mPFC. Scale bar, 40 μm B and C. Single-plane confocal images showing the HCN1-ir ( B1 ), HCN2-ir ( B2 ), HCN4-ir ( B3 ), and GAD65-ir ( C1-C3 ) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). E. Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bars represent 20 μm.
    Figure Legend Snippet: HCN channels are present on GABAergic terminals in the mPFC. A. Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrating the cells in layer 5-6 of the mPFC. Scale bar, 40 μm B and C. Single-plane confocal images showing the HCN1-ir ( B1 ), HCN2-ir ( B2 ), HCN4-ir ( B3 ), and GAD65-ir ( C1-C3 ) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). E. Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bars represent 20 μm.

    Techniques Used: Staining, Marker, Labeling

    2) Product Images from "Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex"

    Article Title: Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex

    Journal: bioRxiv

    doi: 10.1101/2021.05.23.445332

    HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layer 5-6 of mPFC. A-C. Microscopic confocal images showing HCN1-ir ( A ), HCN2-ir ( B ), and HCN4-ir ( C ) locate in PV-ir interneuron in layer 5-6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bar, 20 μm D. High-magnification confocal microscopy images showing that HCN1-ir localize in the soma ( d1 ) and along neurite ( d2-d3 ) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite ( d1 ) is digital magnified for better view of neurite in ( d2 ) and ( d3 ), respectively. Scale bars, 20 μm in ( d1 ) and 1 μm in ( d2 ) and ( d3 ). E. High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digital magnified for better view of neurite in (e2). Scale bars, 20 μm in (e1) and 1 μm in (e2).
    Figure Legend Snippet: HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layer 5-6 of mPFC. A-C. Microscopic confocal images showing HCN1-ir ( A ), HCN2-ir ( B ), and HCN4-ir ( C ) locate in PV-ir interneuron in layer 5-6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bar, 20 μm D. High-magnification confocal microscopy images showing that HCN1-ir localize in the soma ( d1 ) and along neurite ( d2-d3 ) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite ( d1 ) is digital magnified for better view of neurite in ( d2 ) and ( d3 ), respectively. Scale bars, 20 μm in ( d1 ) and 1 μm in ( d2 ) and ( d3 ). E. High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digital magnified for better view of neurite in (e2). Scale bars, 20 μm in (e1) and 1 μm in (e2).

    Techniques Used: Expressing, Staining, Labeling, Confocal Microscopy

    HCN channels are present on GABAergic terminals in the mPFC. A. Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrating the cells in layer 5-6 of the mPFC. Scale bar, 40 μm B and C. Single-plane confocal images showing the HCN1-ir ( B1 ), HCN2-ir ( B2 ), HCN4-ir ( B3 ), and GAD65-ir ( C1-C3 ) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). E. Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bars represent 20 μm.
    Figure Legend Snippet: HCN channels are present on GABAergic terminals in the mPFC. A. Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrating the cells in layer 5-6 of the mPFC. Scale bar, 40 μm B and C. Single-plane confocal images showing the HCN1-ir ( B1 ), HCN2-ir ( B2 ), HCN4-ir ( B3 ), and GAD65-ir ( C1-C3 ) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). E. Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bars represent 20 μm.

    Techniques Used: Staining, Marker, Labeling

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    Alomone Labs polyclonal rabbit rb anti hcn2
    Alteration in HCN channel expression in thalamus following general demyelination. ( A , B ) Immunofluorescence staining of the VB complex (horizontal thalamic sections, 40 μm) comparing the expression of <t>HCN4</t> (A, in red, <t>rb-anti-HCN4,</t> 1:200, Alomone) and <t>HCN2</t> (B, in red, rb-anti-HCN4, 1:200, Alomone) channels between control C3H/HeJ and Day1. The purified ms-anti- neurofilament antibody (SMI312, pan axonal, 1:200, BioLegend) was used as an axonal marker (SMI312, in green). Cell nuclei were stained with DAPI (in blue). ( C , D ) Bar graphs comparing the intensity of the fluorescence signal (using integrated fluorescence intensity values) for SMI312 (C and D upper traces) and HCN4 and HCN2 (lower traces) between the control C3H/HeJ and Day1. ( E ) Immunofluorescence staining of VB in control C3H/HeJ and Day1 with antibodies against TRIP8b (ms-anti-(constant) TRIP8b, 1:50, NeuroMab, in green) and phosphorylated TRIP8b (rb-α-pS237 antibody, 1:100, YenZym). ( F ) Representative bar graph comparing the intensity of the fluorescence signal for total TRIP8b and pS237 between the two groups indicating a significant reduction for phosphorylated TRIP8b, pS237, on Day1. Scale bars indicate 100 μm. VPL, VPM, and TRN stand for ventral-posterior medial, ventral-posterior lateral, and thalamic reticular nucleus, respectively.
    Polyclonal Rabbit Rb Anti Hcn2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    95
    Alomone Labs anti hcn2 antibody
    Effect of chronic training on HCN1, KCN2 and HCN4 protein expression determined by immunocytochemical techniques in sedentary and trained dog. Bar diagram on top left indicates that the relative density of dog cardiomyocytes with HCN4 immunolabelling obtained from the trained group (n=30 cells/6 dogs) is significantly increased compared to that measured in the sedentary group (n=30 cells/6 dogs). Original immunofluorescent images are shown on the left . Bottom panels indicate lack of effect of chronic training on the relative density of dog cardiomyocytes with HCN1 and <t>HCN2</t> immunolabelling (n=30 – 30 cells/6 – 6 dogs for sedentary and trained groups, respectively). Figure 7–Source Data 1 Effect of chronic training on HCN4 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 2 Effect of chronic training on HCN1 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 3 Effect of chronic training on HCN2 protein expression determined by immunocytochemical technique in sedentary and trained dogs.
    Anti Hcn2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hcn2 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hcn2 antibody - by Bioz Stars, 2022-12
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    Image Search Results


    Alteration in HCN channel expression in thalamus following general demyelination. ( A , B ) Immunofluorescence staining of the VB complex (horizontal thalamic sections, 40 μm) comparing the expression of HCN4 (A, in red, rb-anti-HCN4, 1:200, Alomone) and HCN2 (B, in red, rb-anti-HCN4, 1:200, Alomone) channels between control C3H/HeJ and Day1. The purified ms-anti- neurofilament antibody (SMI312, pan axonal, 1:200, BioLegend) was used as an axonal marker (SMI312, in green). Cell nuclei were stained with DAPI (in blue). ( C , D ) Bar graphs comparing the intensity of the fluorescence signal (using integrated fluorescence intensity values) for SMI312 (C and D upper traces) and HCN4 and HCN2 (lower traces) between the control C3H/HeJ and Day1. ( E ) Immunofluorescence staining of VB in control C3H/HeJ and Day1 with antibodies against TRIP8b (ms-anti-(constant) TRIP8b, 1:50, NeuroMab, in green) and phosphorylated TRIP8b (rb-α-pS237 antibody, 1:100, YenZym). ( F ) Representative bar graph comparing the intensity of the fluorescence signal for total TRIP8b and pS237 between the two groups indicating a significant reduction for phosphorylated TRIP8b, pS237, on Day1. Scale bars indicate 100 μm. VPL, VPM, and TRN stand for ventral-posterior medial, ventral-posterior lateral, and thalamic reticular nucleus, respectively.

    Journal: Cerebral Cortex (New York, NY)

    Article Title: Modulation of pacemaker channel function in a model of thalamocortical hyperexcitability by demyelination and cytokines

    doi: 10.1093/cercor/bhab491

    Figure Lengend Snippet: Alteration in HCN channel expression in thalamus following general demyelination. ( A , B ) Immunofluorescence staining of the VB complex (horizontal thalamic sections, 40 μm) comparing the expression of HCN4 (A, in red, rb-anti-HCN4, 1:200, Alomone) and HCN2 (B, in red, rb-anti-HCN4, 1:200, Alomone) channels between control C3H/HeJ and Day1. The purified ms-anti- neurofilament antibody (SMI312, pan axonal, 1:200, BioLegend) was used as an axonal marker (SMI312, in green). Cell nuclei were stained with DAPI (in blue). ( C , D ) Bar graphs comparing the intensity of the fluorescence signal (using integrated fluorescence intensity values) for SMI312 (C and D upper traces) and HCN4 and HCN2 (lower traces) between the control C3H/HeJ and Day1. ( E ) Immunofluorescence staining of VB in control C3H/HeJ and Day1 with antibodies against TRIP8b (ms-anti-(constant) TRIP8b, 1:50, NeuroMab, in green) and phosphorylated TRIP8b (rb-α-pS237 antibody, 1:100, YenZym). ( F ) Representative bar graph comparing the intensity of the fluorescence signal for total TRIP8b and pS237 between the two groups indicating a significant reduction for phosphorylated TRIP8b, pS237, on Day1. Scale bars indicate 100 μm. VPL, VPM, and TRN stand for ventral-posterior medial, ventral-posterior lateral, and thalamic reticular nucleus, respectively.

    Article Snippet: Sections were washed three times for 10 min in PBS and incubated for 2 h in blocking solution (10% normal goat serum, 3% bovine serum albumin (BSA), 0.3% Triton-X100 in PBS) followed by 48 h of incubation at 4 °C with the following primary antibodies: polyclonal rabbit (rb)-anti-HCN2 (1:200), rb-anti-HCN4 (1:200; Alomone Labs), rb-anti-NeuN (neuronal specific marker, 1:1000; Abcam), mouse purified (ms)-anti-neurofilament marker (pan axonal, cocktail, SMI312; 1:200, BioLegend) and ms-anti-Parvalbumin (PV235, 1:500; Swant).

    Techniques: Expressing, Immunofluorescence, Staining, Purification, Marker, Fluorescence

    Human lymphatic vessels predominantly express HCN2. (a) RT‐PCR analysis of HCN2 (229 base pairs) and HCN3 (589 bp) amplified from thoracic duct (TD) and mesenteric lymphatic vessels (ML); HCN1 (597 bp) and HCN4 (232 bp) were consistently amplified from the control human RNA, dorsal root ganglia (DRG) and heart, respectively, while all lymphatic samples were negative. Samples are presented pairwise as reverse transcriptase positive (+) followed by reverse transcriptase negative (−). HCN2 immunoreactivity (red fluorescence, left panels) was observed in smooth muscle cells of (b) human thoracic duct and (c) mesenteric lymphatic vessels. (d) Antibody specificity was confirmed by the absence of staining when the antibody was preincubated with peptide control (green fluorescent nuclear stain). Scale bars denote 50 μm (top) and 100 μm (middle and bottom), * indicates lumen, and right panels present differential interference contrast (DiC) images of the same section.

    Journal: Physiological Reports

    Article Title: Positive chronotropic action of HCN channel antagonism in human collecting lymphatic vessels). Positive chronotropic action of HCN channel antagonism in human collecting lymphatic vessels

    doi: 10.14814/phy2.15401

    Figure Lengend Snippet: Human lymphatic vessels predominantly express HCN2. (a) RT‐PCR analysis of HCN2 (229 base pairs) and HCN3 (589 bp) amplified from thoracic duct (TD) and mesenteric lymphatic vessels (ML); HCN1 (597 bp) and HCN4 (232 bp) were consistently amplified from the control human RNA, dorsal root ganglia (DRG) and heart, respectively, while all lymphatic samples were negative. Samples are presented pairwise as reverse transcriptase positive (+) followed by reverse transcriptase negative (−). HCN2 immunoreactivity (red fluorescence, left panels) was observed in smooth muscle cells of (b) human thoracic duct and (c) mesenteric lymphatic vessels. (d) Antibody specificity was confirmed by the absence of staining when the antibody was preincubated with peptide control (green fluorescent nuclear stain). Scale bars denote 50 μm (top) and 100 μm (middle and bottom), * indicates lumen, and right panels present differential interference contrast (DiC) images of the same section.

    Article Snippet: Thereafter, tissue was permeabilized with 0.25% Triton X‐100 in PBS for 10 min following by blocking with 2% bovine serum albumin (BSA) in PBS for 20 min. Tissue was incubated overnight in a dark, humidified chamber at 4°C with 1:50 rabbit polyclonal anti‐HCN2 (APC‐030‐AR; Alomone, Israel) preconjugated to ATTO‐594 fluorophore.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Fluorescence, Staining

    Effect of chronic training on HCN1, KCN2 and HCN4 protein expression determined by immunocytochemical techniques in sedentary and trained dog. Bar diagram on top left indicates that the relative density of dog cardiomyocytes with HCN4 immunolabelling obtained from the trained group (n=30 cells/6 dogs) is significantly increased compared to that measured in the sedentary group (n=30 cells/6 dogs). Original immunofluorescent images are shown on the left . Bottom panels indicate lack of effect of chronic training on the relative density of dog cardiomyocytes with HCN1 and HCN2 immunolabelling (n=30 – 30 cells/6 – 6 dogs for sedentary and trained groups, respectively). Figure 7–Source Data 1 Effect of chronic training on HCN4 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 2 Effect of chronic training on HCN1 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 3 Effect of chronic training on HCN2 protein expression determined by immunocytochemical technique in sedentary and trained dogs.

    Journal: bioRxiv

    Article Title: Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibilty in a canine model of elite exercise

    doi: 10.1101/2022.07.13.499876

    Figure Lengend Snippet: Effect of chronic training on HCN1, KCN2 and HCN4 protein expression determined by immunocytochemical techniques in sedentary and trained dog. Bar diagram on top left indicates that the relative density of dog cardiomyocytes with HCN4 immunolabelling obtained from the trained group (n=30 cells/6 dogs) is significantly increased compared to that measured in the sedentary group (n=30 cells/6 dogs). Original immunofluorescent images are shown on the left . Bottom panels indicate lack of effect of chronic training on the relative density of dog cardiomyocytes with HCN1 and HCN2 immunolabelling (n=30 – 30 cells/6 – 6 dogs for sedentary and trained groups, respectively). Figure 7–Source Data 1 Effect of chronic training on HCN4 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 2 Effect of chronic training on HCN1 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 3 Effect of chronic training on HCN2 protein expression determined by immunocytochemical technique in sedentary and trained dogs.

    Article Snippet: After the incubation period, cells were labelled overnight at 4°C with anti-KChIP2 (Alomone, #APC-142, RRID:AB_2756744), anti-Kv4.3 (Alomone, #APC-017, RRID:AB_2040178), anti-HCN1 (Alomone, #APC-056, RRID:AB_2039900), anti-HCN2 (Alomone, #APC-030, RRID:AB_2313726) and anti-HCN4 (Alomone, #APC-052, RRID:AB_2039906) primary antibody diluted to 1:50.

    Techniques: Expressing