hcn2  (Alomone Labs)


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    Name:
    Anti HCN2 Antibody
    Description:
    Anti HCN2 Antibody APC 030 is a highly specific antibody directed against an epitope of the human protein The antibody can be used in western blot immunoprecipitation immunohistochemistry and immunocytochemistry applications It has been designed to recognize HCN2 from human rat and mouse samples
    Catalog Number:
    APC-030
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs hcn2
    Anti HCN2 Antibody
    Anti HCN2 Antibody APC 030 is a highly specific antibody directed against an epitope of the human protein The antibody can be used in western blot immunoprecipitation immunohistochemistry and immunocytochemistry applications It has been designed to recognize HCN2 from human rat and mouse samples
    https://www.bioz.com/result/hcn2/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn2 - by Bioz Stars, 2021-09
    95/100 stars

    Images

    1) Product Images from "CA2+-ACTIVATED ADENYLYL CYCLASE 1 INTRODUCES CA2+-DEPENDENCE TO BETA-ADRENERGIC STIMULATION OF HCN2 CURRENT"

    Article Title: CA2+-ACTIVATED ADENYLYL CYCLASE 1 INTRODUCES CA2+-DEPENDENCE TO BETA-ADRENERGIC STIMULATION OF HCN2 CURRENT

    Journal: Journal of molecular and cellular cardiology

    doi: 10.1016/j.yjmcc.2012.03.010

    Effect of over-expression of AC isoforms on HCN2 current. A . Original recordings of HCN2. The current was evoked by applying hyperpolarizing voltages from −25 to −85 mV for 5 seconds. B. Average fractional activation of measured HCN2 current. The solid lines are the fits to the Boltzmann function. AC1 activation relation differs from GFP and AC6 (p
    Figure Legend Snippet: Effect of over-expression of AC isoforms on HCN2 current. A . Original recordings of HCN2. The current was evoked by applying hyperpolarizing voltages from −25 to −85 mV for 5 seconds. B. Average fractional activation of measured HCN2 current. The solid lines are the fits to the Boltzmann function. AC1 activation relation differs from GFP and AC6 (p

    Techniques Used: Over Expression, Activation Assay

    A. Expression of recombinant adenylyl cyclase in NRVM. Cultures were co-infected with AdmHCN2 and one of the following: AdGFP, FLAG-tagged AdAC1 or FLAG-tagged AdAC6. 72 hours later cells were harvested, and the soluble membrane fraction was isolated. Soluble membranes from cultures infected with AdGFP, AdAC1 or AdAC6 were incubated with anti-FLAG or anti-HCN2 antibodies, separated by PAGE and probed with appropriate antibodies. B . Expression of endogenous HCN2 in NRVM and its co-immunoprecipitation with overexpressed adenylyl cyclases. The top panel shows detection of HCN2 in membrane fraction and the bottom panel – in anti-FLAG-immunoprecipitates.
    Figure Legend Snippet: A. Expression of recombinant adenylyl cyclase in NRVM. Cultures were co-infected with AdmHCN2 and one of the following: AdGFP, FLAG-tagged AdAC1 or FLAG-tagged AdAC6. 72 hours later cells were harvested, and the soluble membrane fraction was isolated. Soluble membranes from cultures infected with AdGFP, AdAC1 or AdAC6 were incubated with anti-FLAG or anti-HCN2 antibodies, separated by PAGE and probed with appropriate antibodies. B . Expression of endogenous HCN2 in NRVM and its co-immunoprecipitation with overexpressed adenylyl cyclases. The top panel shows detection of HCN2 in membrane fraction and the bottom panel – in anti-FLAG-immunoprecipitates.

    Techniques Used: Expressing, Recombinant, Infection, Isolation, Incubation, Polyacrylamide Gel Electrophoresis, Immunoprecipitation

    2) Product Images from "Adenosine and dopamine oppositely modulate a hyperpolarization‐activated current I h in chemosensory neurons of the rat carotid body in co‐culture"

    Article Title: Adenosine and dopamine oppositely modulate a hyperpolarization‐activated current I h in chemosensory neurons of the rat carotid body in co‐culture

    Journal: The Journal of Physiology

    doi: 10.1113/JP274743

    Localization of HCN4 and HCN2 subunits in tissue sections of rat petrosal ganglia In A , confocal immunofluorescence labelling revealed that HCN4 immunoreactivity (HCN4‐ir) is present in many petrosal neurons, and co‐localizes with several neurons expressing tyrosine hydroxylase (TH)‐ir ( B ), a marker for chemoafferent neurons; merged images are shown in C . Note co‐localization of TH‐ir and HCN4‐ir in several neurons in boxed area ( n = 3). In D , many petrosal neurons are positive for HCN2‐ir, but there is no evidence for a significant co‐localization with TH‐ir in the boxed region ( E and F ; n = 3). In control experiments omission of the primary antibody, or preincubation with blocking peptide (see Methods), resulted in the absence of all immunostaining ( n ]
    Figure Legend Snippet: Localization of HCN4 and HCN2 subunits in tissue sections of rat petrosal ganglia In A , confocal immunofluorescence labelling revealed that HCN4 immunoreactivity (HCN4‐ir) is present in many petrosal neurons, and co‐localizes with several neurons expressing tyrosine hydroxylase (TH)‐ir ( B ), a marker for chemoafferent neurons; merged images are shown in C . Note co‐localization of TH‐ir and HCN4‐ir in several neurons in boxed area ( n = 3). In D , many petrosal neurons are positive for HCN2‐ir, but there is no evidence for a significant co‐localization with TH‐ir in the boxed region ( E and F ; n = 3). In control experiments omission of the primary antibody, or preincubation with blocking peptide (see Methods), resulted in the absence of all immunostaining ( n ]

    Techniques Used: Immunofluorescence, Expressing, Marker, Blocking Assay, Immunostaining

    3) Product Images from "Differential expression of hyperpolarization-activated cyclic nucleotide-gated channel subunits during hippocampal development in the mouse"

    Article Title: Differential expression of hyperpolarization-activated cyclic nucleotide-gated channel subunits during hippocampal development in the mouse

    Journal: Molecular Brain

    doi: 10.1186/s13041-015-0103-4

    Expression of HCN2 in PV-positive cells of the hippocampus at P21. (A-B, D-F, H-J, L-N, P) : The images show HCN2 labeling in different fields of the hippocampus and the DG. (A, E, I) : Note that HCN2 expression was more prominent in the sl-m and sp of the CA, particularly in CA3. Interestingly, the alveus did not show HCN2 expression at this developmental stage. In addition, the sl-m and sp of the CA3 (I) presented stronger labeling than in the CA1 (A) and the CA2 (E) . Most PV-immunopositive cells located in the sp of the CA1 (A, B, D) , CA2 (E, F, H) , CA3 (I, J, L) , and hilus of the DG (M, N, P) co-expressed HCN2. A - B , D - F , H - J , L - N , P : white arrows indicate PV-positive/HCN2-positive cells. C , G , K , O : DAPI staining. Scale bars = 20 μm.
    Figure Legend Snippet: Expression of HCN2 in PV-positive cells of the hippocampus at P21. (A-B, D-F, H-J, L-N, P) : The images show HCN2 labeling in different fields of the hippocampus and the DG. (A, E, I) : Note that HCN2 expression was more prominent in the sl-m and sp of the CA, particularly in CA3. Interestingly, the alveus did not show HCN2 expression at this developmental stage. In addition, the sl-m and sp of the CA3 (I) presented stronger labeling than in the CA1 (A) and the CA2 (E) . Most PV-immunopositive cells located in the sp of the CA1 (A, B, D) , CA2 (E, F, H) , CA3 (I, J, L) , and hilus of the DG (M, N, P) co-expressed HCN2. A - B , D - F , H - J , L - N , P : white arrows indicate PV-positive/HCN2-positive cells. C , G , K , O : DAPI staining. Scale bars = 20 μm.

    Techniques Used: Expressing, Labeling, Staining

    Expression of HCN subunits in the hippocampus at P0 and P7. (A-1) : At P0, the expression of HCN1 was strong in the alveus and so , sp , sl , and sr of the CA, and in the GCL of the dentate gyrus (DG). (E-1) : HCN2 expression at P0 was observed in the alveus , sp , sl , and sr of the CA, and in the GCL of the DG. (I-1) : HCN4 showed a similar pattern of expression to HCN1 at P0. Note that migrating cells (yellow arrows) from the ventricular zone (vz, white arrows) expressed all HCN isoforms, but the expression of HCN1 and HCN2 subunits was more prominent than that of HCN4. At P7, immunolabeling for HCN1 (A-2) , HCN2 (E-2) , and HCN4 (I-2) was observed in the sp and slm of the CA, and in the gcl of the DG. HCN1 (B, C, D) and HCN2 (F, G, H) subunits were expressed in neuronal somata, but not in astrocytes. Labeling for HCN4 was observed in the slm , as well as in the border of the slm with the molecular layer ( ml ) of the DG. (B, F, J) : Double immunofluorescence with GFAP showed that most GFAP-positive astrocytes were also immunolabeled with HCN4 in the slm, and in the border of the ml in the DG. B , C , D : yellow arrows indicate HCN1 labeling, white arrows indicate GFAP labeling. F , G , H : yellow arrows indicate HCN2 labeling. J , K , L : white arrow indicates an astrocyte double-labeled for HCN4 and GFAP. Abbreviations: GCL (or gcl), granule cell layer; sl, stratum lucidum; slm, stratum lacunosum moleculare; so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum. Scale bars = 20 μm.
    Figure Legend Snippet: Expression of HCN subunits in the hippocampus at P0 and P7. (A-1) : At P0, the expression of HCN1 was strong in the alveus and so , sp , sl , and sr of the CA, and in the GCL of the dentate gyrus (DG). (E-1) : HCN2 expression at P0 was observed in the alveus , sp , sl , and sr of the CA, and in the GCL of the DG. (I-1) : HCN4 showed a similar pattern of expression to HCN1 at P0. Note that migrating cells (yellow arrows) from the ventricular zone (vz, white arrows) expressed all HCN isoforms, but the expression of HCN1 and HCN2 subunits was more prominent than that of HCN4. At P7, immunolabeling for HCN1 (A-2) , HCN2 (E-2) , and HCN4 (I-2) was observed in the sp and slm of the CA, and in the gcl of the DG. HCN1 (B, C, D) and HCN2 (F, G, H) subunits were expressed in neuronal somata, but not in astrocytes. Labeling for HCN4 was observed in the slm , as well as in the border of the slm with the molecular layer ( ml ) of the DG. (B, F, J) : Double immunofluorescence with GFAP showed that most GFAP-positive astrocytes were also immunolabeled with HCN4 in the slm, and in the border of the ml in the DG. B , C , D : yellow arrows indicate HCN1 labeling, white arrows indicate GFAP labeling. F , G , H : yellow arrows indicate HCN2 labeling. J , K , L : white arrow indicates an astrocyte double-labeled for HCN4 and GFAP. Abbreviations: GCL (or gcl), granule cell layer; sl, stratum lucidum; slm, stratum lacunosum moleculare; so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum. Scale bars = 20 μm.

    Techniques Used: Expressing, Immunolabeling, Labeling, Immunofluorescence

    Expression of HCN2 in PV-positive cells of the hippocampus at P56. (A-B, D, E-F, H-J, L-N, P) : Most PV-immunopositive cells located in the CA1 (A-B, D) , CA2 (E, F, H) , CA3 (I, J, L) , and DG (M, N, P) were labeled with HCN2. (A, E, I) : HCN2 expression was most prominent in the sl-m of the CA1 (A) , as well as in the sp of the CA2 (E) and CA3 (I) . Interestingly, at this stage the alveus showed HCN2 labeling, contrasting with its expression pattern at P21. A - B , D , E - F , H - J , L - N , P : white arrows indicate PV-positive/HCN2-positive cells. C , G , K , O : DAPI staining. Scale bars = 20 μm.
    Figure Legend Snippet: Expression of HCN2 in PV-positive cells of the hippocampus at P56. (A-B, D, E-F, H-J, L-N, P) : Most PV-immunopositive cells located in the CA1 (A-B, D) , CA2 (E, F, H) , CA3 (I, J, L) , and DG (M, N, P) were labeled with HCN2. (A, E, I) : HCN2 expression was most prominent in the sl-m of the CA1 (A) , as well as in the sp of the CA2 (E) and CA3 (I) . Interestingly, at this stage the alveus showed HCN2 labeling, contrasting with its expression pattern at P21. A - B , D , E - F , H - J , L - N , P : white arrows indicate PV-positive/HCN2-positive cells. C , G , K , O : DAPI staining. Scale bars = 20 μm.

    Techniques Used: Expressing, Labeling, Staining

    Expression of HCN subunits in the hippocampal region at E14.5. HCN1 (A) , HCN2 (D) , and HCN4 (G) immunolabeling was more evident in the intermediate zone (iz) than in the ventricular zone (vz). In the hippocampal iz, labeling for HCN2 and HCN4 subunits was higher than for HCN1. B , E , H : DAPI staining in the nucleus. C , F , I : merged images of HCN immunolabeling and DAPI stain. Scale bars = 20 μm.
    Figure Legend Snippet: Expression of HCN subunits in the hippocampal region at E14.5. HCN1 (A) , HCN2 (D) , and HCN4 (G) immunolabeling was more evident in the intermediate zone (iz) than in the ventricular zone (vz). In the hippocampal iz, labeling for HCN2 and HCN4 subunits was higher than for HCN1. B , E , H : DAPI staining in the nucleus. C , F , I : merged images of HCN immunolabeling and DAPI stain. Scale bars = 20 μm.

    Techniques Used: Expressing, Immunolabeling, Labeling, Staining

    Expression of HCN subunits in doublecortin (DCX)-positive cells of the dentate gyrus at P21. (A-L) : These images show our results on double immunofluorescence for HCN subunits and DCX. In the DCX-immunopositive subgranular zone we did not find labeling for HCN1 (A-D) , HCN2 (E-H) , or HCN4 (I-L) . Scale bars = 20 μm.
    Figure Legend Snippet: Expression of HCN subunits in doublecortin (DCX)-positive cells of the dentate gyrus at P21. (A-L) : These images show our results on double immunofluorescence for HCN subunits and DCX. In the DCX-immunopositive subgranular zone we did not find labeling for HCN1 (A-D) , HCN2 (E-H) , or HCN4 (I-L) . Scale bars = 20 μm.

    Techniques Used: Expressing, Immunofluorescence, Labeling

    4) Product Images from "Characterization of small fiber pathology in a mouse model of Fabry disease"

    Article Title: Characterization of small fiber pathology in a mouse model of Fabry disease

    Journal: eLife

    doi: 10.7554/eLife.39300

    Expression, function, and phenotypic reflection of hyperpolarization-activated cyclic nucleotide-gated ion channels in α-galactosidase A deficient mice. ( A ) Boxplots show the results of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene expression in dorsal root ganglia (DRG) of young (3 months) and old (≥12 months) wildtype (WT) and α-galactosidase A deficient (GLA KO) mice. No intergroup difference was found. ( B–E ) Photomicrographs illustrate immunoreactivity of antibodies against HCN2 in DRG of young and old WT and GLA KO mice; ( F ) shows the result of quantification. Old GLA KO and WT mice showed greated HCN2 immunoreactivity compared to young littermates (p
    Figure Legend Snippet: Expression, function, and phenotypic reflection of hyperpolarization-activated cyclic nucleotide-gated ion channels in α-galactosidase A deficient mice. ( A ) Boxplots show the results of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene expression in dorsal root ganglia (DRG) of young (3 months) and old (≥12 months) wildtype (WT) and α-galactosidase A deficient (GLA KO) mice. No intergroup difference was found. ( B–E ) Photomicrographs illustrate immunoreactivity of antibodies against HCN2 in DRG of young and old WT and GLA KO mice; ( F ) shows the result of quantification. Old GLA KO and WT mice showed greated HCN2 immunoreactivity compared to young littermates (p

    Techniques Used: Expressing, Mouse Assay

    5) Product Images from "Differential Distribution and Function of Hyperpolarization-Activated Channels in Sensory Neurons and Mechanosensitive Fibers"

    Article Title: Differential Distribution and Function of Hyperpolarization-Activated Channels in Sensory Neurons and Mechanosensitive Fibers

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.5156-03.2004

    HCN immunoreactivity in club endings of myelinated fibers accompanied by fine unmyelinated fibers. Left, HCN1 (FITC) colocalized (yellow) with peripherin (Rhodamine Red-X) in a club ending but not with fine unmyelinated fiber (arrowhead). Center, HCN2 (Rhodamine Red-X) appeared in both the fine unmyelinated fibers and in a club ending of a myelinated fiber (yellow). Right, Both club endings and fine fibers (arrowhead) labeled with FITC-NF mixture also expressed HCN4 (Rhodamine Red-X). HCN2 and HCN4 (Rhodamine Red-X) are also found in cells surrounding the fibers.
    Figure Legend Snippet: HCN immunoreactivity in club endings of myelinated fibers accompanied by fine unmyelinated fibers. Left, HCN1 (FITC) colocalized (yellow) with peripherin (Rhodamine Red-X) in a club ending but not with fine unmyelinated fiber (arrowhead). Center, HCN2 (Rhodamine Red-X) appeared in both the fine unmyelinated fibers and in a club ending of a myelinated fiber (yellow). Right, Both club endings and fine fibers (arrowhead) labeled with FITC-NF mixture also expressed HCN4 (Rhodamine Red-X). HCN2 and HCN4 (Rhodamine Red-X) are also found in cells surrounding the fibers.

    Techniques Used: Labeling

    HCN1, HCN2, and HCN4 immunoreactivity in nodose neurons. A–C , HCN immunoreactivity identified in 6–10 μm sections of nodose ganglion. HCN1 immunoreactivity was localized to a small subpopulation of neurons and, in most of these cells, was heavily localized at the plasma membrane ( A ). HCN2 ( B ) and HCN4 ( C ) immunoreactivity was present in all neurons in the ganglion. D–F , Single confocal sections through cultured nodose neurons selected for expression of HCN1 ( D ), HCN2 ( E ), and HCN4 ( F ). Heavy labeling at the membrane is again shown for HCN1 ( D ). Patches of HCN2 and HCN4 immunoreactivity were located at the cell perimeter; examples are indicated by the arrows ( E, F ). The light microscopic differential interference contrast image is also shown for each neuron. The calibration bar in C also applies to A and B , whereas calibration in F applies to E . The antibodies were preabsorbed with the immunizing peptide as shown in the figure. A control for nonspecific staining omitted the primary Ab (data not shown).
    Figure Legend Snippet: HCN1, HCN2, and HCN4 immunoreactivity in nodose neurons. A–C , HCN immunoreactivity identified in 6–10 μm sections of nodose ganglion. HCN1 immunoreactivity was localized to a small subpopulation of neurons and, in most of these cells, was heavily localized at the plasma membrane ( A ). HCN2 ( B ) and HCN4 ( C ) immunoreactivity was present in all neurons in the ganglion. D–F , Single confocal sections through cultured nodose neurons selected for expression of HCN1 ( D ), HCN2 ( E ), and HCN4 ( F ). Heavy labeling at the membrane is again shown for HCN1 ( D ). Patches of HCN2 and HCN4 immunoreactivity were located at the cell perimeter; examples are indicated by the arrows ( E, F ). The light microscopic differential interference contrast image is also shown for each neuron. The calibration bar in C also applies to A and B , whereas calibration in F applies to E . The antibodies were preabsorbed with the immunizing peptide as shown in the figure. A control for nonspecific staining omitted the primary Ab (data not shown).

    Techniques Used: Cell Culture, Expressing, Labeling, Staining

    HCN1, HCN2, HCN3, and HCN4 mRNA is expressed in nodose ganglia. PCR products resulting from the amplification of first-strand cDNA prepared with (+) or without (–) RT from nodose ganglia or brain poly A+ RNA with HCN1-, HCN2-, HCN3-, and HCN4-specific oligonucleotides were separated by electrophoresis and transferred to nylon membranes (Ambion). After Southern hybridization with 32 P-labeled specific internal oligomers, the autoradiogram showed a positive signal for all four channels from nodose and rat brain in the (+) RT lanes and no signals in the control (–) RT. The oligonucleotide probes amplify cDNA of 641 bp for HCN1, 638 bp for HCN2, 509 bp for HCN3, and 635 bp for HCN4.
    Figure Legend Snippet: HCN1, HCN2, HCN3, and HCN4 mRNA is expressed in nodose ganglia. PCR products resulting from the amplification of first-strand cDNA prepared with (+) or without (–) RT from nodose ganglia or brain poly A+ RNA with HCN1-, HCN2-, HCN3-, and HCN4-specific oligonucleotides were separated by electrophoresis and transferred to nylon membranes (Ambion). After Southern hybridization with 32 P-labeled specific internal oligomers, the autoradiogram showed a positive signal for all four channels from nodose and rat brain in the (+) RT lanes and no signals in the control (–) RT. The oligonucleotide probes amplify cDNA of 641 bp for HCN1, 638 bp for HCN2, 509 bp for HCN3, and 635 bp for HCN4.

    Techniques Used: Polymerase Chain Reaction, Amplification, Electrophoresis, Hybridization, Labeling

    HCN immunoreactivity in aortic baroreceptor terminals of myelinated fibers. Top, A collapsed Z-series stack of 0.4 μm confocal sections through a bush baroreceptor terminal shows localization of HCN1 (left) and PGP9.5 (right). PGP9.5 is a ubiquitin hydrolase expressed in neuronal–neuroendocrine cells. Middle, Bush ending is colabeled with HCN2 on the left and the neurofilament mixture on the right. Bottom, HCN4 immunoreactivity on the left is localized to the bush ending identified using the neurofilament mixture (right).
    Figure Legend Snippet: HCN immunoreactivity in aortic baroreceptor terminals of myelinated fibers. Top, A collapsed Z-series stack of 0.4 μm confocal sections through a bush baroreceptor terminal shows localization of HCN1 (left) and PGP9.5 (right). PGP9.5 is a ubiquitin hydrolase expressed in neuronal–neuroendocrine cells. Middle, Bush ending is colabeled with HCN2 on the left and the neurofilament mixture on the right. Bottom, HCN4 immunoreactivity on the left is localized to the bush ending identified using the neurofilament mixture (right).

    Techniques Used:

    6) Product Images from "Alterations in hyperpolarization-activated cyclic nucleotidegated cation channel (HCN) expression in the hippocampus following pilocarpine-induced status epilepticus"

    Article Title: Alterations in hyperpolarization-activated cyclic nucleotidegated cation channel (HCN) expression in the hippocampus following pilocarpine-induced status epilepticus

    Journal: BMB Reports

    doi: 10.5483/BMBRep.2012.45.11.091

    The HCN2 immunoreactivity in the hippocampus in the normal and epileptic animal models following SE. HCN2 expressions are detected in some interneurons of CA1-3 and the dentate gyrus (arrows in panel A2-A4), whereas its immunoreactivity is observed in the granule cell layer of the dentate gyrus (A1 and A4). At 12 hrs following SE, HCN2 immunoreactivities in interneuronal populations and the granule cell layer are reduced compared to the control (arrows in panel B2-B4). These expressions are enhanced in CA1 and the dentate gyrus at day 7 following SE (C1-C4). After this time window, HCN2 immunoreactive interneurons are re-declined until 5 weeks following SE (D1-D4). Bar = 280 μm (panels A1, B1, C1, and D1), 50 μm (panels A2-A4, B2-B4, C2-C4, and D2-D4). Quantitative analyses of HCN2 immunoreactivity in the normal and epileptic hippocampi following SE (E, mean ± S.E.M). Significant differences from the control group, *P < 0.05, **P < 0.01.
    Figure Legend Snippet: The HCN2 immunoreactivity in the hippocampus in the normal and epileptic animal models following SE. HCN2 expressions are detected in some interneurons of CA1-3 and the dentate gyrus (arrows in panel A2-A4), whereas its immunoreactivity is observed in the granule cell layer of the dentate gyrus (A1 and A4). At 12 hrs following SE, HCN2 immunoreactivities in interneuronal populations and the granule cell layer are reduced compared to the control (arrows in panel B2-B4). These expressions are enhanced in CA1 and the dentate gyrus at day 7 following SE (C1-C4). After this time window, HCN2 immunoreactive interneurons are re-declined until 5 weeks following SE (D1-D4). Bar = 280 μm (panels A1, B1, C1, and D1), 50 μm (panels A2-A4, B2-B4, C2-C4, and D2-D4). Quantitative analyses of HCN2 immunoreactivity in the normal and epileptic hippocampi following SE (E, mean ± S.E.M). Significant differences from the control group, *P < 0.05, **P < 0.01.

    Techniques Used:

    Related Articles

    Incubation:

    Article Title: In vivo cardiac pacemaker function of differentiated human mesenchymal stem cells from adipose tissue transplanted into porcine hearts
    Article Snippet: .. The sections were incubated with first antibodies overnight at 4°C in 2% BSA/PBS: anti-HCN1 (IgG, Alomone Labs) 1:200, anti-HCN2 (IgG, Alomone Labs) 1:200, anti-HCN4 (IgG, Alomone Labs) 1:200, anti-Cav1.2 (IgG, Abcam) 1:100, anti-Cx31.9 (IgG, Santa Cruz Biotechnology) 1:200, anti-Cx45 (IgG, Santa Cruz Biotechnology) 1:200 and anti-calprotectin (Chemicon, Temecula, CA, United States) 1:200. ..

    Article Title: Oral Administration of Penicillin or Streptomycin May Alter Serum Serotonin Level and Intestinal Motility via Different Mechanisms
    Article Snippet: .. Then tissue sections were incubated with rabbit anti-HCN2 (1:1000, Alomone, Israel) or goat anti-5-HT (1:2000, Abcam, Cambridge, MA, United States) at 4°C for 48 h. After four rinses with PBS, the sections were incubated with donkey anti-rabbit Alexa Fluor 488 secondary antibody (1:1000, Invitrogen, Eugene, OR, United States) or donkey anti-goat Alexa Fluor 568 secondary antibody (1:1000, Invitrogen, Eugene, OR, United States) for 2 h at room temperature. ..

    Article Title: Toward Biological Pacing by Cellular Delivery of Hcn2/SkM1
    Article Snippet: .. Cells were permeabilized with 0.1% Triton X-100 for 8 min and incubated overnight with the following antibodies diluted in 4% goat serum: mouse α-SkM1 antibody (Sigma-Aldrich, S9568, 1: 200) and rabbit α-HCN2 antibody (Alomone, APC-030, 1:200). ..

    Imaging:

    Article Title: Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases †
    Article Snippet: .. For imaging of endogenous HCN2, cells were labeled with mouse anti-β tubulin (1 : 1000, Sigma, T8328) and rabbit anti-HCN2 (1 : 800, Alomone, APC-030) marked with Alexa 488-conjugated anti-mouse IgG (1 : 500; Invitrogen, A11029) and Alexa 647-conjugated anti-rabbit IgG (1 : 500; Invitrogen, A21245), respectively. ..

    Article Title: Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases †
    Article Snippet: .. For imaging of ectopically expressed HCN2, cells were transfected with mEos::HCN2 using Turbofect reagent prior to fixation and labelling with anti-HCN2 antibodies. ..

    Labeling:

    Article Title: Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases †
    Article Snippet: .. For imaging of endogenous HCN2, cells were labeled with mouse anti-β tubulin (1 : 1000, Sigma, T8328) and rabbit anti-HCN2 (1 : 800, Alomone, APC-030) marked with Alexa 488-conjugated anti-mouse IgG (1 : 500; Invitrogen, A11029) and Alexa 647-conjugated anti-rabbit IgG (1 : 500; Invitrogen, A21245), respectively. ..

    Transfection:

    Article Title: Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases †
    Article Snippet: .. For imaging of ectopically expressed HCN2, cells were transfected with mEos::HCN2 using Turbofect reagent prior to fixation and labelling with anti-HCN2 antibodies. ..

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  • 95
    Alomone Labs rabbit α hcn2 antibody
    Domains mediating the interaction of VAPB with <t>HCN2.</t> A ) Schematic illustration of a HCN subunit. The CNBD and some of the truncation constructs studied are indicated. B ) All truncation constructs exhibited a positive interaction, evident from growth on -LWHA dropout medium. C ) Representative current traces and the relative currents for different C-terminal deletions expressed alone or with VAPB. D ) Representative current traces and the relative current amplitudes for the N-terminal truncated NTK HCN2 expressed alone or with VAPB. E ) Relative current amplitudes of NTK HCN2 HA Ex (extracellular HA-tag) expressed alone or with VAPB. F ) Relative surface expression of NTK HCN2 HA Ex expressed alone or with VAPB analyzed as relative light units (RLUs). G ) Schematic illustration, representative traces, and currents of a HCN2 channel chimera with the N terminus of HCN4 ( HCN4-N HCN2) expressed alone or with VAPB. H ) Relative currents of HCN2 expressed alone or coexpressed with VAPB (1.7 ± 0.1), TM VAPB (1.6 ± 0.2), the MSP domain (MSP VAPB ), the MSP with half of the CC domain (MSP-CC 0.5 VAPB ), or with the complete CC domain (MSP-CC VAPB ). I , J ) Relative current amplitudes of HCN2 HA Ex expressed alone or with TM VAPB (1.3 ± 0.1) ( I ) and the respective changes in the relative surface membrane expression analyzed as RLUs, using a single cell chemiluminescence assay (TM VAPB 1.8 ± 0.2) ( J ). All data are presented as means ± sem . The number of experiments ( n ) is indicated in the respective bar graphs. N.s., not significant. * P
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    Domains mediating the interaction of VAPB with HCN2. A ) Schematic illustration of a HCN subunit. The CNBD and some of the truncation constructs studied are indicated. B ) All truncation constructs exhibited a positive interaction, evident from growth on -LWHA dropout medium. C ) Representative current traces and the relative currents for different C-terminal deletions expressed alone or with VAPB. D ) Representative current traces and the relative current amplitudes for the N-terminal truncated NTK HCN2 expressed alone or with VAPB. E ) Relative current amplitudes of NTK HCN2 HA Ex (extracellular HA-tag) expressed alone or with VAPB. F ) Relative surface expression of NTK HCN2 HA Ex expressed alone or with VAPB analyzed as relative light units (RLUs). G ) Schematic illustration, representative traces, and currents of a HCN2 channel chimera with the N terminus of HCN4 ( HCN4-N HCN2) expressed alone or with VAPB. H ) Relative currents of HCN2 expressed alone or coexpressed with VAPB (1.7 ± 0.1), TM VAPB (1.6 ± 0.2), the MSP domain (MSP VAPB ), the MSP with half of the CC domain (MSP-CC 0.5 VAPB ), or with the complete CC domain (MSP-CC VAPB ). I , J ) Relative current amplitudes of HCN2 HA Ex expressed alone or with TM VAPB (1.3 ± 0.1) ( I ) and the respective changes in the relative surface membrane expression analyzed as RLUs, using a single cell chemiluminescence assay (TM VAPB 1.8 ± 0.2) ( J ). All data are presented as means ± sem . The number of experiments ( n ) is indicated in the respective bar graphs. N.s., not significant. * P

    Journal: The FASEB Journal

    Article Title: The VAMP-associated protein VAPB is required for cardiac and neuronal pacemaker channel function

    doi: 10.1096/fj.201800246R

    Figure Lengend Snippet: Domains mediating the interaction of VAPB with HCN2. A ) Schematic illustration of a HCN subunit. The CNBD and some of the truncation constructs studied are indicated. B ) All truncation constructs exhibited a positive interaction, evident from growth on -LWHA dropout medium. C ) Representative current traces and the relative currents for different C-terminal deletions expressed alone or with VAPB. D ) Representative current traces and the relative current amplitudes for the N-terminal truncated NTK HCN2 expressed alone or with VAPB. E ) Relative current amplitudes of NTK HCN2 HA Ex (extracellular HA-tag) expressed alone or with VAPB. F ) Relative surface expression of NTK HCN2 HA Ex expressed alone or with VAPB analyzed as relative light units (RLUs). G ) Schematic illustration, representative traces, and currents of a HCN2 channel chimera with the N terminus of HCN4 ( HCN4-N HCN2) expressed alone or with VAPB. H ) Relative currents of HCN2 expressed alone or coexpressed with VAPB (1.7 ± 0.1), TM VAPB (1.6 ± 0.2), the MSP domain (MSP VAPB ), the MSP with half of the CC domain (MSP-CC 0.5 VAPB ), or with the complete CC domain (MSP-CC VAPB ). I , J ) Relative current amplitudes of HCN2 HA Ex expressed alone or with TM VAPB (1.3 ± 0.1) ( I ) and the respective changes in the relative surface membrane expression analyzed as RLUs, using a single cell chemiluminescence assay (TM VAPB 1.8 ± 0.2) ( J ). All data are presented as means ± sem . The number of experiments ( n ) is indicated in the respective bar graphs. N.s., not significant. * P

    Article Snippet: Untagged HCN2 protein was detected with rabbit α-HCN2 antibody (APC-030, 1:300; Alomone Labs, Jerusalem, Israel) and peroxidase-conjugated goat α-rabbit IgG antibody (32460, 1:2000; Thermo Fisher Scientific) as the secondary antibody.

    Techniques: Construct, Expressing, Chemiluminescence Immunoassay

    VAPB determines surface expression and dendritic localization of HCN2. A ) Live cell imaging of HeLa cells transfected with an N-terminally EGFP-tagged HCN2 carrying an extracellular HA-epitope ( EGFP HCN2 HA Ex ) alone or cotransfected with VAPB or the TM segment of VAPB (TM VAPB ). B ) Chemiluminescence assays of fixed non-permeabilized HeLa cells, analyzing the surface expression as relative light units (RLUs) for EGFP HCN2 HA Ex alone and after cotransfection with VAPB (1.6 ± 0.1). Upper inset illustrates a representative control Western blot showing an unaltered HCN2 prote in expression. C ) Chemiluminescence surface expression assay as in B , but using TM VAPB (1.6 ± 0.1). D ) Immunocytochemistry of HA VAPB transfected cortical neurons. Endogenous HCN2 (green) is colocalizing (white) with HA VAPB (magenta) in the soma and dendrites. Anti–MAP2-staining illustrating an intact neuronal network and dendrites (blue). E ) Immunocytochemistry experiment as in D , but transfecting the ALS8 mutation HA VAPB P56S (magenta), leading to an aggregation of VAPB P56S in the soma of the neurons. Also, HCN2 fluorescence (green) was focused in the soma and dendritic localization was lost, despite an intact neuronal network (α-MAP2, blue). Scale bars, 20 µm ( A , D , E ). All data are presented as means ± sem . The number of experiments ( n ) is indicated in the respective bar graphs. ** P

    Journal: The FASEB Journal

    Article Title: The VAMP-associated protein VAPB is required for cardiac and neuronal pacemaker channel function

    doi: 10.1096/fj.201800246R

    Figure Lengend Snippet: VAPB determines surface expression and dendritic localization of HCN2. A ) Live cell imaging of HeLa cells transfected with an N-terminally EGFP-tagged HCN2 carrying an extracellular HA-epitope ( EGFP HCN2 HA Ex ) alone or cotransfected with VAPB or the TM segment of VAPB (TM VAPB ). B ) Chemiluminescence assays of fixed non-permeabilized HeLa cells, analyzing the surface expression as relative light units (RLUs) for EGFP HCN2 HA Ex alone and after cotransfection with VAPB (1.6 ± 0.1). Upper inset illustrates a representative control Western blot showing an unaltered HCN2 prote in expression. C ) Chemiluminescence surface expression assay as in B , but using TM VAPB (1.6 ± 0.1). D ) Immunocytochemistry of HA VAPB transfected cortical neurons. Endogenous HCN2 (green) is colocalizing (white) with HA VAPB (magenta) in the soma and dendrites. Anti–MAP2-staining illustrating an intact neuronal network and dendrites (blue). E ) Immunocytochemistry experiment as in D , but transfecting the ALS8 mutation HA VAPB P56S (magenta), leading to an aggregation of VAPB P56S in the soma of the neurons. Also, HCN2 fluorescence (green) was focused in the soma and dendritic localization was lost, despite an intact neuronal network (α-MAP2, blue). Scale bars, 20 µm ( A , D , E ). All data are presented as means ± sem . The number of experiments ( n ) is indicated in the respective bar graphs. ** P

    Article Snippet: Untagged HCN2 protein was detected with rabbit α-HCN2 antibody (APC-030, 1:300; Alomone Labs, Jerusalem, Israel) and peroxidase-conjugated goat α-rabbit IgG antibody (32460, 1:2000; Thermo Fisher Scientific) as the secondary antibody.

    Techniques: Expressing, Live Cell Imaging, Transfection, Cotransfection, Western Blot, Immunocytochemistry, Staining, Mutagenesis, Fluorescence

    Codistribution of VAPs with HCN2 and contribution to thalamic I h . A – E ), Distribution of HCN2, VAPB, and VAPA mRNA in mouse brain and spinal cord. ISH analysis of HCN2, VAPB, and VAPA using DIG-labeled riboprobes, revealing mRNA expression of VAPB in cortical areas ( A ), hippocampus ( B ), thalamus ( C ), cerebellum ( D ) (arrows point to interneurons in the granular layer), and spinal cord ( E ). Note the overlapping distribution of VAPB with HCN2 and VAPA mRNA. Am, amygdala; CA, cornu ammonis; DG, dentate gyrus; DH, dorsal horn; gcl, granule cell layer; Hb, habenulae; ic, internal capsule; LG, lateral geniculate ncl.; m, molecular cell layer; pcl, Purkinje cell layer; RTh, reticular thalamic ncl.; Sth, subthalamic ncl.; VB, ventrobasal thalamus; Th, thalamus; VH, ventral horn. F ) Representative current traces elicited in slice patch-clamp experiments of the ventrobasal thalamus (VB) of wild-type animals (control) and VAPB −/− mice. G ) The I h current was significantly reduced in VAPB −/− mice (15.4 ± 1.1 pA/pF) compared with control animals (22.2 ± 2.3 pA/pF). H ) Average activation curves of the VB I h current for control and VAPB −/− mice. V 1/2 of activation for control (−91.6 ± 1.3 mV, n = 8) and VAPB −/− (−87.5 ± 1.2 mV, n = 7). Scale bars: 500 µm ( A–C , E ), 100 µm ( D ). All data are presented as means ± sem . The number of experiments ( n ) is indicated in the respective bar graphs. * P

    Journal: The FASEB Journal

    Article Title: The VAMP-associated protein VAPB is required for cardiac and neuronal pacemaker channel function

    doi: 10.1096/fj.201800246R

    Figure Lengend Snippet: Codistribution of VAPs with HCN2 and contribution to thalamic I h . A – E ), Distribution of HCN2, VAPB, and VAPA mRNA in mouse brain and spinal cord. ISH analysis of HCN2, VAPB, and VAPA using DIG-labeled riboprobes, revealing mRNA expression of VAPB in cortical areas ( A ), hippocampus ( B ), thalamus ( C ), cerebellum ( D ) (arrows point to interneurons in the granular layer), and spinal cord ( E ). Note the overlapping distribution of VAPB with HCN2 and VAPA mRNA. Am, amygdala; CA, cornu ammonis; DG, dentate gyrus; DH, dorsal horn; gcl, granule cell layer; Hb, habenulae; ic, internal capsule; LG, lateral geniculate ncl.; m, molecular cell layer; pcl, Purkinje cell layer; RTh, reticular thalamic ncl.; Sth, subthalamic ncl.; VB, ventrobasal thalamus; Th, thalamus; VH, ventral horn. F ) Representative current traces elicited in slice patch-clamp experiments of the ventrobasal thalamus (VB) of wild-type animals (control) and VAPB −/− mice. G ) The I h current was significantly reduced in VAPB −/− mice (15.4 ± 1.1 pA/pF) compared with control animals (22.2 ± 2.3 pA/pF). H ) Average activation curves of the VB I h current for control and VAPB −/− mice. V 1/2 of activation for control (−91.6 ± 1.3 mV, n = 8) and VAPB −/− (−87.5 ± 1.2 mV, n = 7). Scale bars: 500 µm ( A–C , E ), 100 µm ( D ). All data are presented as means ± sem . The number of experiments ( n ) is indicated in the respective bar graphs. * P

    Article Snippet: Untagged HCN2 protein was detected with rabbit α-HCN2 antibody (APC-030, 1:300; Alomone Labs, Jerusalem, Israel) and peroxidase-conjugated goat α-rabbit IgG antibody (32460, 1:2000; Thermo Fisher Scientific) as the secondary antibody.

    Techniques: In Situ Hybridization, Labeling, Expressing, Patch Clamp, Mouse Assay, Activation Assay

    VAPB selectively increases HCN1 and HCN2 currents. A ) Y2H direct interaction assay. Transformation control (-LW), leucine, and tryptophan dropout. Interaction read-out (-LWHA), additional dropout of histidine and adenine. pAL-Alg5, positive control. pPR3-N, negative control. B ) Topology of VAPB. C ) GST VAPB pull-down of HCN2 EGFP using transfected HeLa cells. D ) GST VAPA, GST VAMP1, or GST VAMP2 pull-down of HCN2 EGFP using transfected HeLa cells. E ) GST VAPA pull-down of HCN2 and endogenous VAPB, using HCN2 EGFP transfected HeLa cells. F ) Pull-down of in vitro translated HCN2 (untagged). G ) Pull-down of HCN2 from rat brain lysates. H , I ) Representative currents ( H ) of HCN2 expressed in oocytes alone or with VAPB and the relative current amplitudes ( I ) analyzed over 3 d. J ) Relative currents of HCN1, HCN2, and HCN4 alone or coexpressed with VAPB. K , L ) Relative currents of different potassium channels ( K ) coexpressed with VAPB and of HCN2 ( L ) coexpressed with VAPA, VAPB, or VAPC. M ) Relative currents of HCN2 coexpressed with a mixture of VAPA/B (1:1). N ) Representative macropatch recordings in different configurations: on cell (o.c.), inside-out after patch excision (i.o.), and after application of 100 µM cAMP (i.o.+100 µM cAMP). O , P ) Activation curves for HCN2 alone ( n = 6) ( O ), recorded as in N , or after coexpression with VAPB ( n = 8) ( P ). Q ) V 1/2 values for HCN2 expressed alone or with VAPB in different patch modes. R ) Relative currents of HCN2 HA Ex alone or with VAPB. S ) Relative surface expression of HCN2 HA Ex expressed alone or with VAPB, analyzed as relative light units (RLUs). T ) Relative currents of HCN2 expressed alone or with VAPB or VAPB P56S . All data are presented as means ± sem . The number of cells ( n ) is indicated in the bar graphs. N.s., not significant. * P

    Journal: The FASEB Journal

    Article Title: The VAMP-associated protein VAPB is required for cardiac and neuronal pacemaker channel function

    doi: 10.1096/fj.201800246R

    Figure Lengend Snippet: VAPB selectively increases HCN1 and HCN2 currents. A ) Y2H direct interaction assay. Transformation control (-LW), leucine, and tryptophan dropout. Interaction read-out (-LWHA), additional dropout of histidine and adenine. pAL-Alg5, positive control. pPR3-N, negative control. B ) Topology of VAPB. C ) GST VAPB pull-down of HCN2 EGFP using transfected HeLa cells. D ) GST VAPA, GST VAMP1, or GST VAMP2 pull-down of HCN2 EGFP using transfected HeLa cells. E ) GST VAPA pull-down of HCN2 and endogenous VAPB, using HCN2 EGFP transfected HeLa cells. F ) Pull-down of in vitro translated HCN2 (untagged). G ) Pull-down of HCN2 from rat brain lysates. H , I ) Representative currents ( H ) of HCN2 expressed in oocytes alone or with VAPB and the relative current amplitudes ( I ) analyzed over 3 d. J ) Relative currents of HCN1, HCN2, and HCN4 alone or coexpressed with VAPB. K , L ) Relative currents of different potassium channels ( K ) coexpressed with VAPB and of HCN2 ( L ) coexpressed with VAPA, VAPB, or VAPC. M ) Relative currents of HCN2 coexpressed with a mixture of VAPA/B (1:1). N ) Representative macropatch recordings in different configurations: on cell (o.c.), inside-out after patch excision (i.o.), and after application of 100 µM cAMP (i.o.+100 µM cAMP). O , P ) Activation curves for HCN2 alone ( n = 6) ( O ), recorded as in N , or after coexpression with VAPB ( n = 8) ( P ). Q ) V 1/2 values for HCN2 expressed alone or with VAPB in different patch modes. R ) Relative currents of HCN2 HA Ex alone or with VAPB. S ) Relative surface expression of HCN2 HA Ex expressed alone or with VAPB, analyzed as relative light units (RLUs). T ) Relative currents of HCN2 expressed alone or with VAPB or VAPB P56S . All data are presented as means ± sem . The number of cells ( n ) is indicated in the bar graphs. N.s., not significant. * P

    Article Snippet: Untagged HCN2 protein was detected with rabbit α-HCN2 antibody (APC-030, 1:300; Alomone Labs, Jerusalem, Israel) and peroxidase-conjugated goat α-rabbit IgG antibody (32460, 1:2000; Thermo Fisher Scientific) as the secondary antibody.

    Techniques: Transformation Assay, Positive Control, Negative Control, Transfection, In Vitro, Activation Assay, Expressing

    Effect of over-expression of AC isoforms on HCN2 current. A . Original recordings of HCN2. The current was evoked by applying hyperpolarizing voltages from −25 to −85 mV for 5 seconds. B. Average fractional activation of measured HCN2 current. The solid lines are the fits to the Boltzmann function. AC1 activation relation differs from GFP and AC6 (p

    Journal: Journal of molecular and cellular cardiology

    Article Title: CA2+-ACTIVATED ADENYLYL CYCLASE 1 INTRODUCES CA2+-DEPENDENCE TO BETA-ADRENERGIC STIMULATION OF HCN2 CURRENT

    doi: 10.1016/j.yjmcc.2012.03.010

    Figure Lengend Snippet: Effect of over-expression of AC isoforms on HCN2 current. A . Original recordings of HCN2. The current was evoked by applying hyperpolarizing voltages from −25 to −85 mV for 5 seconds. B. Average fractional activation of measured HCN2 current. The solid lines are the fits to the Boltzmann function. AC1 activation relation differs from GFP and AC6 (p

    Article Snippet: To explore the possibility of direct interaction with HCN2 channels, we immunoprecipitated AC with anti-FLAG antibodies, separated the obtained proteins by PAGE and probed with anti-HCN2 antibodies ( ; middle panel).

    Techniques: Over Expression, Activation Assay

    A. Expression of recombinant adenylyl cyclase in NRVM. Cultures were co-infected with AdmHCN2 and one of the following: AdGFP, FLAG-tagged AdAC1 or FLAG-tagged AdAC6. 72 hours later cells were harvested, and the soluble membrane fraction was isolated. Soluble membranes from cultures infected with AdGFP, AdAC1 or AdAC6 were incubated with anti-FLAG or anti-HCN2 antibodies, separated by PAGE and probed with appropriate antibodies. B . Expression of endogenous HCN2 in NRVM and its co-immunoprecipitation with overexpressed adenylyl cyclases. The top panel shows detection of HCN2 in membrane fraction and the bottom panel – in anti-FLAG-immunoprecipitates.

    Journal: Journal of molecular and cellular cardiology

    Article Title: CA2+-ACTIVATED ADENYLYL CYCLASE 1 INTRODUCES CA2+-DEPENDENCE TO BETA-ADRENERGIC STIMULATION OF HCN2 CURRENT

    doi: 10.1016/j.yjmcc.2012.03.010

    Figure Lengend Snippet: A. Expression of recombinant adenylyl cyclase in NRVM. Cultures were co-infected with AdmHCN2 and one of the following: AdGFP, FLAG-tagged AdAC1 or FLAG-tagged AdAC6. 72 hours later cells were harvested, and the soluble membrane fraction was isolated. Soluble membranes from cultures infected with AdGFP, AdAC1 or AdAC6 were incubated with anti-FLAG or anti-HCN2 antibodies, separated by PAGE and probed with appropriate antibodies. B . Expression of endogenous HCN2 in NRVM and its co-immunoprecipitation with overexpressed adenylyl cyclases. The top panel shows detection of HCN2 in membrane fraction and the bottom panel – in anti-FLAG-immunoprecipitates.

    Article Snippet: To explore the possibility of direct interaction with HCN2 channels, we immunoprecipitated AC with anti-FLAG antibodies, separated the obtained proteins by PAGE and probed with anti-HCN2 antibodies ( ; middle panel).

    Techniques: Expressing, Recombinant, Infection, Isolation, Incubation, Polyacrylamide Gel Electrophoresis, Immunoprecipitation

    HCN2 and HCN4 are predominant in ORNs

    Journal: The Journal of Physiology

    Article Title: Hyperpolarisation-activated cyclic nucleotide-gated channels regulate the spontaneous firing rate of olfactory receptor neurons and affect glomerular formation in mice

    doi: 10.1113/jphysiol.2012.247361

    Figure Lengend Snippet: HCN2 and HCN4 are predominant in ORNs

    Article Snippet: The following primary antibodies were used in the present study: anti-HCN1 (1:1000, Dr Shigemoto, National Institute for Physiological Sciences, Japan), anti-HCN2, anti-HCN3, anti-HCN4 (1:500, Alomone Labs, Jerusalem, Israel) and anti-olfactory marker protein (OMP, 1:400, Wako, Japan).

    Techniques: