kchip2  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs kchip2
    Kchip2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kchip2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kchip2 - by Bioz Stars, 2022-12
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs rabbit polyclonal anti kv4 2 antibody
    <t>Kv4.3</t> immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with <t>antibodies</t> directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.
    Rabbit Polyclonal Anti Kv4 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti kv4 2 antibody/product/Alomone Labs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti kv4 2 antibody - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    Image Search Results


    Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Article Snippet: The following antibodies were used for immunostaining only (all at a dilution of 1:400): rabbit polyclonal anti-Kv4.2 antibody (Alomone, RRID:AB_2040176 ) generated against amino acids 454–469 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877281 ) generated against a synthetic peptide corresponding to amino acids 209–225 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877425 ) generated against a fusion protein corresponding to amino acids 471-630 of rat Kv4.2; rabbit polyclonal anti-Kv1.4 antibody (Alomone, RRID:AB_2040153 ) generated against a GST fusion protein corresponding to amino acid 589-655 of rat Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877317 ) generated against a synthetic peptide corresponding to amino acids 13–37 of rat brain Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877393 ) generated against a fusion peptide of amino acids 336-370 of rat Kv1.4; and mouse monoclonal IgG1 anti-NF-L antibody (Millipore MAB1615) directed against enzymatically dephosphorylated pig neurofilament-L (70 kDa).

    Techniques:

    Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Article Snippet: The following antibodies were used for immunostaining only (all at a dilution of 1:400): rabbit polyclonal anti-Kv4.2 antibody (Alomone, RRID:AB_2040176 ) generated against amino acids 454–469 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877281 ) generated against a synthetic peptide corresponding to amino acids 209–225 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877425 ) generated against a fusion protein corresponding to amino acids 471-630 of rat Kv4.2; rabbit polyclonal anti-Kv1.4 antibody (Alomone, RRID:AB_2040153 ) generated against a GST fusion protein corresponding to amino acid 589-655 of rat Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877317 ) generated against a synthetic peptide corresponding to amino acids 13–37 of rat brain Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877393 ) generated against a fusion peptide of amino acids 336-370 of rat Kv1.4; and mouse monoclonal IgG1 anti-NF-L antibody (Millipore MAB1615) directed against enzymatically dephosphorylated pig neurofilament-L (70 kDa).

    Techniques: Binding Assay, Microscopy, Western Blot, Fluorescence, Molecular Weight, Migration

    Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Article Snippet: The following antibodies were used for immunostaining only (all at a dilution of 1:400): rabbit polyclonal anti-Kv4.2 antibody (Alomone, RRID:AB_2040176 ) generated against amino acids 454–469 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877281 ) generated against a synthetic peptide corresponding to amino acids 209–225 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877425 ) generated against a fusion protein corresponding to amino acids 471-630 of rat Kv4.2; rabbit polyclonal anti-Kv1.4 antibody (Alomone, RRID:AB_2040153 ) generated against a GST fusion protein corresponding to amino acid 589-655 of rat Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877317 ) generated against a synthetic peptide corresponding to amino acids 13–37 of rat brain Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877393 ) generated against a fusion peptide of amino acids 336-370 of rat Kv1.4; and mouse monoclonal IgG1 anti-NF-L antibody (Millipore MAB1615) directed against enzymatically dephosphorylated pig neurofilament-L (70 kDa).

    Techniques: Immunoprecipitation, Western Blot, Electrophoresis, Molecular Weight, Migration