anti kv3 1b  (Alomone Labs)


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    Structured Review

    Alomone Labs anti kv3 1b
    Expression of <t>Kv3</t> and Kv4 subunits in GAD65-GFP mouse dorsal horn. (A–C) GFP +ve neurones (A) and <t>Kv3.1b</t> subunit immunfluorescence (B) localized to superficial dorsal horn. Merged images (C) indicate a significant overlap between GFP +ve neurones and Kv3.1b immunoreactivity (grey arrows) although examples exist where GFP fluorescence (white arrows, green) and Kv3.1b fluorescence (black arrows, red) are clearly not co-localized. (D–F) GFP +ve neurones (D) and Kv3.3 subunit immunofluorescence (E) localized to superficial dorsal horn. Merged images (C) indicate no significant overlap between GFP +ve neurones (white arrows, green and Kv3.3 immunoreactivity (black arrows, red). (G–I) GFP +ve neurones (G) and Kv4.2 immunoreactivity (H) localized to superficial dorsal horn. Merged images (I) indicate no association between GFP fluorescence (white arrows, green) and immunoreactivity for Kv4.2 (back arrows, red). (J–L) Merged images for GAD 65 +ve neurones (J, green) and Kv 4.3 (K, red) indicate expression by separate populations. Scale bars: A–F, 20 μm; G–L, 50 μm.
    Anti Kv3 1b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Kv3.1b and Kv3.3 channel subunit expression in murine spinal dorsal horn GABAergic interneurones"

    Article Title: Kv3.1b and Kv3.3 channel subunit expression in murine spinal dorsal horn GABAergic interneurones

    Journal: Journal of Chemical Neuroanatomy

    doi: 10.1016/j.jchemneu.2011.02.003

    Expression of Kv3 and Kv4 subunits in GAD65-GFP mouse dorsal horn. (A–C) GFP +ve neurones (A) and Kv3.1b subunit immunfluorescence (B) localized to superficial dorsal horn. Merged images (C) indicate a significant overlap between GFP +ve neurones and Kv3.1b immunoreactivity (grey arrows) although examples exist where GFP fluorescence (white arrows, green) and Kv3.1b fluorescence (black arrows, red) are clearly not co-localized. (D–F) GFP +ve neurones (D) and Kv3.3 subunit immunofluorescence (E) localized to superficial dorsal horn. Merged images (C) indicate no significant overlap between GFP +ve neurones (white arrows, green and Kv3.3 immunoreactivity (black arrows, red). (G–I) GFP +ve neurones (G) and Kv4.2 immunoreactivity (H) localized to superficial dorsal horn. Merged images (I) indicate no association between GFP fluorescence (white arrows, green) and immunoreactivity for Kv4.2 (back arrows, red). (J–L) Merged images for GAD 65 +ve neurones (J, green) and Kv 4.3 (K, red) indicate expression by separate populations. Scale bars: A–F, 20 μm; G–L, 50 μm.
    Figure Legend Snippet: Expression of Kv3 and Kv4 subunits in GAD65-GFP mouse dorsal horn. (A–C) GFP +ve neurones (A) and Kv3.1b subunit immunfluorescence (B) localized to superficial dorsal horn. Merged images (C) indicate a significant overlap between GFP +ve neurones and Kv3.1b immunoreactivity (grey arrows) although examples exist where GFP fluorescence (white arrows, green) and Kv3.1b fluorescence (black arrows, red) are clearly not co-localized. (D–F) GFP +ve neurones (D) and Kv3.3 subunit immunofluorescence (E) localized to superficial dorsal horn. Merged images (C) indicate no significant overlap between GFP +ve neurones (white arrows, green and Kv3.3 immunoreactivity (black arrows, red). (G–I) GFP +ve neurones (G) and Kv4.2 immunoreactivity (H) localized to superficial dorsal horn. Merged images (I) indicate no association between GFP fluorescence (white arrows, green) and immunoreactivity for Kv4.2 (back arrows, red). (J–L) Merged images for GAD 65 +ve neurones (J, green) and Kv 4.3 (K, red) indicate expression by separate populations. Scale bars: A–F, 20 μm; G–L, 50 μm.

    Techniques Used: Expressing, Fluorescence, Immunofluorescence

    Expression of Kv3 and Kv4 subunits in GAD67-GFP (Δneo) mouse dorsal horn. (A–C) GFP +ve neurones (A) and Kv3.1b subunit immunfluorescence (B) localized to superficial dorsal horn. Merged images (C) indicate that some GFP +ve neurones also express Kv3.1b immunoreactivity (grey arrows) although examples exist where GFP fluorescence (white arrows, green) and Kv3.1b fluorescence (black arrows, red) are not co-localized. (D–F) GFP +ve neurones (D) and Kv3.3 subunit immunofluorescence (E) localized to superficial dorsal horn. Merged images (C) indicate no significant overlap between GFP +ve neurones (white arrows, green and Kv3.3 immunoreactivity (black arrows, red). (G–H) GFP +ve neurones (G) and Kv4.2 immunoreactivity (H) localized to superficial dorsal horn. (J–K) GFP +ve neurones (J) and Kv4.2 immunoreactivity (K) localized to superficial dorsal horn. Merged images (I and L) indicate no association between GFP fluorescence (white arrows, green) and immunoreactivity for Kv4.2 or Kv4.3 (black arrows, red). Scale bars all 20 μm.
    Figure Legend Snippet: Expression of Kv3 and Kv4 subunits in GAD67-GFP (Δneo) mouse dorsal horn. (A–C) GFP +ve neurones (A) and Kv3.1b subunit immunfluorescence (B) localized to superficial dorsal horn. Merged images (C) indicate that some GFP +ve neurones also express Kv3.1b immunoreactivity (grey arrows) although examples exist where GFP fluorescence (white arrows, green) and Kv3.1b fluorescence (black arrows, red) are not co-localized. (D–F) GFP +ve neurones (D) and Kv3.3 subunit immunofluorescence (E) localized to superficial dorsal horn. Merged images (C) indicate no significant overlap between GFP +ve neurones (white arrows, green and Kv3.3 immunoreactivity (black arrows, red). (G–H) GFP +ve neurones (G) and Kv4.2 immunoreactivity (H) localized to superficial dorsal horn. (J–K) GFP +ve neurones (J) and Kv4.2 immunoreactivity (K) localized to superficial dorsal horn. Merged images (I and L) indicate no association between GFP fluorescence (white arrows, green) and immunoreactivity for Kv4.2 or Kv4.3 (black arrows, red). Scale bars all 20 μm.

    Techniques Used: Expressing, Fluorescence, Immunofluorescence

    2) Product Images from "Kv3.1b Is a Novel Component of CNS Nodes"

    Article Title: Kv3.1b Is a Novel Component of CNS Nodes

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.23-11-04509.2003

    Distribution and interaction with ankyrin G of Kv3.1b and Kv3.2 subunits. A, B , Membrane proteins (100 μg) from Kv3.1-deficient mouse brain and rat brain, spinal cord, optic nerve, and sciatic nerve were fractioned in SDS gel and immunoblotted for Kv3.1b ( A ) and Kv3.2 ( B ). Kv3.1b was expressed in rat brain and spinal cord, and to a lesser extents in the optic nerve. No band was observed in Kv3.1-deficient mouse. Kv3.2 was detected in the mouse and rat brain and to a lesser extent in the rat spinal cord. C , Brain and spinal cord membranes (200 μg) were immunoprecipitated separately for Kv3.1b or Kv3.2 and then immunoblotted for Kv3.1b. Note that Kv3.1b coimmunoprecipitates extensively with Kv3.2 in the brain but not in the spinal cord. D, E , Brain and spinal cord membranes (200 μg) were immunoprecipitated with rabbit anti-ankyrin G (Ank-G), rabbit anti-Kv3.1b, rabbit anti-Kv3.2, or mouse anti-Kv1.2 antibodies, then immunoblotted for Kv3.1b ( D ) or ankyrin G ( E ). Brain membrane (BM) was used as a positive control. Kv3.1b coimmunoprecipitates with ankyrin G and Kv3.2, but not Kv1.2 ( D ). A low-molecular-weight isoform of ankyrin G was pulled down with Kv3.1b, but not with either Kv3.2 or Kv1.2 ( E ). Molecular weight markers are shown on the right in kilodaltons.
    Figure Legend Snippet: Distribution and interaction with ankyrin G of Kv3.1b and Kv3.2 subunits. A, B , Membrane proteins (100 μg) from Kv3.1-deficient mouse brain and rat brain, spinal cord, optic nerve, and sciatic nerve were fractioned in SDS gel and immunoblotted for Kv3.1b ( A ) and Kv3.2 ( B ). Kv3.1b was expressed in rat brain and spinal cord, and to a lesser extents in the optic nerve. No band was observed in Kv3.1-deficient mouse. Kv3.2 was detected in the mouse and rat brain and to a lesser extent in the rat spinal cord. C , Brain and spinal cord membranes (200 μg) were immunoprecipitated separately for Kv3.1b or Kv3.2 and then immunoblotted for Kv3.1b. Note that Kv3.1b coimmunoprecipitates extensively with Kv3.2 in the brain but not in the spinal cord. D, E , Brain and spinal cord membranes (200 μg) were immunoprecipitated with rabbit anti-ankyrin G (Ank-G), rabbit anti-Kv3.1b, rabbit anti-Kv3.2, or mouse anti-Kv1.2 antibodies, then immunoblotted for Kv3.1b ( D ) or ankyrin G ( E ). Brain membrane (BM) was used as a positive control. Kv3.1b coimmunoprecipitates with ankyrin G and Kv3.2, but not Kv1.2 ( D ). A low-molecular-weight isoform of ankyrin G was pulled down with Kv3.1b, but not with either Kv3.2 or Kv1.2 ( E ). Molecular weight markers are shown on the right in kilodaltons.

    Techniques Used: SDS-Gel, Immunoprecipitation, Positive Control, Molecular Weight

    3) Product Images from "The Axon–Dendrite Targeting of Kv3 (Shaw) Channels Is Determined by a Targeting Motif That Associates with the T1 Domain and Ankyrin G"

    Article Title: The Axon–Dendrite Targeting of Kv3 (Shaw) Channels Is Determined by a Targeting Motif That Associates with the T1 Domain and Ankyrin G

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.3675-07.2007

    Ankyrin G differentially regulates polarized targeting of Kv3.1 splice variants. A – G , Hippocampal neurons at 7 DIV were cotransfected with ankyrin G and Kv3.1 constructs, fixed, and stained with anti-HA antibodies under permeabilized conditions. A , Kv3.1aHA (red) did not colocalize with ankyrin G-GFP (green) in axons. B , Kv3.1bHA (red) colocalized with ankyrin G-GFP (green) in p roximal axons. C , The fluorescence intensity profiles along the axons in A and B indicated by arrows. Arrowheads, The fluorescence peaks attributable to crossing dendrites. D , E , The levels of Kv3.1aHA ( D ) and Kv3.1bHA ( E ) in axons markedly increased and modestly decreased, respectively, in the presence of MB–GFP (green). F , The fluorescence intensity profiles along the axons in D and E indicated by arrows. G , Summary of polarized targeting of Kv3.1aHA and Kv3.1bHA in the presence of GFP and MB–GFP. F axon / F dendrite is average fluorescence intensity in axons/average fluorescence intensity in dendrites. H , Endogenous Kv3.1b channels were localized to distal axons (arrows), in contrast to the localization of endogenous Nav channels in proximal axons. Hippocampal neurons at 30 DIV were costained with polyclonal anti-Kv3.1b (green) and monoclonal anti-pan-Nav channel (red) antibodies. It is important to note that Kv3.1b also localizes in soma and proximal dendrites. I , Fluorescence intensity profiles along the axon indicated by arrows in H . Distance 0, Soma. Scale bars, 100 μm. Student's t test used in the comparison of two groups. * p
    Figure Legend Snippet: Ankyrin G differentially regulates polarized targeting of Kv3.1 splice variants. A – G , Hippocampal neurons at 7 DIV were cotransfected with ankyrin G and Kv3.1 constructs, fixed, and stained with anti-HA antibodies under permeabilized conditions. A , Kv3.1aHA (red) did not colocalize with ankyrin G-GFP (green) in axons. B , Kv3.1bHA (red) colocalized with ankyrin G-GFP (green) in p roximal axons. C , The fluorescence intensity profiles along the axons in A and B indicated by arrows. Arrowheads, The fluorescence peaks attributable to crossing dendrites. D , E , The levels of Kv3.1aHA ( D ) and Kv3.1bHA ( E ) in axons markedly increased and modestly decreased, respectively, in the presence of MB–GFP (green). F , The fluorescence intensity profiles along the axons in D and E indicated by arrows. G , Summary of polarized targeting of Kv3.1aHA and Kv3.1bHA in the presence of GFP and MB–GFP. F axon / F dendrite is average fluorescence intensity in axons/average fluorescence intensity in dendrites. H , Endogenous Kv3.1b channels were localized to distal axons (arrows), in contrast to the localization of endogenous Nav channels in proximal axons. Hippocampal neurons at 30 DIV were costained with polyclonal anti-Kv3.1b (green) and monoclonal anti-pan-Nav channel (red) antibodies. It is important to note that Kv3.1b also localizes in soma and proximal dendrites. I , Fluorescence intensity profiles along the axon indicated by arrows in H . Distance 0, Soma. Scale bars, 100 μm. Student's t test used in the comparison of two groups. * p

    Techniques Used: Construct, Staining, Fluorescence

    4) Product Images from "Math5 expression and function in the central auditory system"

    Article Title: Math5 expression and function in the central auditory system

    Journal: Molecular and cellular neurosciences

    doi: 10.1016/j.mcn.2007.09.006

    Math5-lacZ is coexpressed with bushy cell markers in the ventral cochlear nucleus A-H. Cryosections through the VCN of M5 +/− mice (panels A, C, E, G) and M5 −/− mice (panels B, D, F, H) were immunostained for β-galactosidase (red) neuronal antigens (green). A-D. The Kv1.1 and Kv3.1b potassium channels are expressed by all Math5-lacZ cells (arrowheads) and a roughly equal number of lacZ -negative cells (arrows). The co-labeled cells represent a subset of globular and spherical bushy cells within the VCN. E, F. The Kcnq4 channel is similarly expressed by all Math5-lacZ positive cells (arrowhead) and a larger number of lacZ -negative cells (arrow). G, H. Calbindin is expressed by a subset of Math5-lacZ positive cells in the VCN (arrowhead). Additional cells are positive for calbindin only (open arrow) or β-gal only (closed arrow). This indicates that the Math5 -expressing cell population is heterogeneous. Abbreviations: VCN, ventral cochlear nucleus. Scale bar, 100 μm.
    Figure Legend Snippet: Math5-lacZ is coexpressed with bushy cell markers in the ventral cochlear nucleus A-H. Cryosections through the VCN of M5 +/− mice (panels A, C, E, G) and M5 −/− mice (panels B, D, F, H) were immunostained for β-galactosidase (red) neuronal antigens (green). A-D. The Kv1.1 and Kv3.1b potassium channels are expressed by all Math5-lacZ cells (arrowheads) and a roughly equal number of lacZ -negative cells (arrows). The co-labeled cells represent a subset of globular and spherical bushy cells within the VCN. E, F. The Kcnq4 channel is similarly expressed by all Math5-lacZ positive cells (arrowhead) and a larger number of lacZ -negative cells (arrow). G, H. Calbindin is expressed by a subset of Math5-lacZ positive cells in the VCN (arrowhead). Additional cells are positive for calbindin only (open arrow) or β-gal only (closed arrow). This indicates that the Math5 -expressing cell population is heterogeneous. Abbreviations: VCN, ventral cochlear nucleus. Scale bar, 100 μm.

    Techniques Used: Mouse Assay, Labeling, Expressing

    5) Product Images from "Kinesin I transports tetramerized Kv3 channels through the axon initial segment via direct binding"

    Article Title: Kinesin I transports tetramerized Kv3 channels through the axon initial segment via direct binding

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    doi: 10.1523/JNEUROSCI.3565-10.2010

    KIF5B-YFP assists CFP-Kv3.1a and CFP-Kv3.1b to penetrate the AIS and enter distal axons via direct binding revealed by the FRET imaging
    Figure Legend Snippet: KIF5B-YFP assists CFP-Kv3.1a and CFP-Kv3.1b to penetrate the AIS and enter distal axons via direct binding revealed by the FRET imaging

    Techniques Used: Binding Assay, Imaging

    Knocking down endogenous KIF5B by siRNA reduces the axonal level of endogenous Kv3.1b
    Figure Legend Snippet: Knocking down endogenous KIF5B by siRNA reduces the axonal level of endogenous Kv3.1b

    Techniques Used:

    6) Product Images from "Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K+ channels"

    Article Title: Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K+ channels

    Journal: eLife

    doi: 10.7554/eLife.66491

    Kv3.1b membrane localization requires AnkR. ( A ) Immunolabeling of 12-month-old somatosensory cortex from Ank1 F/F and Ank1 F/F ;Dlx5/6-Cre mice using antibodies against AnkR (red) and Kv3.1b (green). Scalebar, 50 μm. ( B ) Immunoblots of brain homogenates from three 12-month-old control and three 12-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. ( C ) Quantification of Kv3.1b protein normalized to NFM. Error bars indicate mean ± SEM. N=3/group. ( D ) Immunolabeling of 1-month-old somatosensory cortex from Ank1 F/F and Ank1 F/F ;Dlx5/6-Cre mice using antibodies against AnkR (red) and Kv3.1 (green). Scalebar, 50 μm. ( E ) Immunolabeling of 1-month-old somatosensory cortex from Ank1 F/F and Ank1 F/F ;Dlx5/6-Cre mice using antibodies against AnkR (red) and Kv3.2 (green). Scalebar, 50 μm. ( F ) Quantification of Kv3.1 immunofluorescence intensity in somatosensory cortex of control and Ank1 F/F ;Dlx5/6-Cre mice. Error bars indicate mean ± SEM. N=3/group. ( G ) Quantification of Kv3.2 immunofluorescence intensity in somatosensory cortex of control and Ank1 F/F ;Dlx5/6-Cre mice. Error bars indicate mean ± SEM. N=3/group. ( H ) Immunolabeling of 1-month-old somatosensory cortex from Ank1 F/F mice using antibodies against AnkR (red) and Kv3.3 (green). Scalebar, 25 μm. ( I ) Immunostaining of Ank1 F/F spinal cord using antibodies against AnkR (red) and Kv3.1b (green). Scalebars, 10 μm and 1 μm. ( J ) Immunostaining of ventral root nodes of Ranvier in Ank3 +/+ and Ank3 F/F ;Chat-Cre mice using antibodies against AnkR (red), Kv3.3 (green), and NFasc (blue). Scalebar, 1 μm. ( K ) The specific K + channels and spectrins found at nodes of Ranvier is dictated by the Ankyrin scaffold to which they bind. Source data related to Figure 6—figure supplement 1 .
    Figure Legend Snippet: Kv3.1b membrane localization requires AnkR. ( A ) Immunolabeling of 12-month-old somatosensory cortex from Ank1 F/F and Ank1 F/F ;Dlx5/6-Cre mice using antibodies against AnkR (red) and Kv3.1b (green). Scalebar, 50 μm. ( B ) Immunoblots of brain homogenates from three 12-month-old control and three 12-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. ( C ) Quantification of Kv3.1b protein normalized to NFM. Error bars indicate mean ± SEM. N=3/group. ( D ) Immunolabeling of 1-month-old somatosensory cortex from Ank1 F/F and Ank1 F/F ;Dlx5/6-Cre mice using antibodies against AnkR (red) and Kv3.1 (green). Scalebar, 50 μm. ( E ) Immunolabeling of 1-month-old somatosensory cortex from Ank1 F/F and Ank1 F/F ;Dlx5/6-Cre mice using antibodies against AnkR (red) and Kv3.2 (green). Scalebar, 50 μm. ( F ) Quantification of Kv3.1 immunofluorescence intensity in somatosensory cortex of control and Ank1 F/F ;Dlx5/6-Cre mice. Error bars indicate mean ± SEM. N=3/group. ( G ) Quantification of Kv3.2 immunofluorescence intensity in somatosensory cortex of control and Ank1 F/F ;Dlx5/6-Cre mice. Error bars indicate mean ± SEM. N=3/group. ( H ) Immunolabeling of 1-month-old somatosensory cortex from Ank1 F/F mice using antibodies against AnkR (red) and Kv3.3 (green). Scalebar, 25 μm. ( I ) Immunostaining of Ank1 F/F spinal cord using antibodies against AnkR (red) and Kv3.1b (green). Scalebars, 10 μm and 1 μm. ( J ) Immunostaining of ventral root nodes of Ranvier in Ank3 +/+ and Ank3 F/F ;Chat-Cre mice using antibodies against AnkR (red), Kv3.3 (green), and NFasc (blue). Scalebar, 1 μm. ( K ) The specific K + channels and spectrins found at nodes of Ranvier is dictated by the Ankyrin scaffold to which they bind. Source data related to Figure 6—figure supplement 1 .

    Techniques Used: Immunolabeling, Mouse Assay, Western Blot, Immunofluorescence, Immunostaining

    AnkR is highly expressed in the perisomatic region of Pv + interneurons which are surrounded by PNNs, a specialized ECM structure. AnkR is a scaffolding protein that binds to and stabilizes PNN-associated CAMs (including NrCAM and PlexinA4) and ion channels (including Kv3.1b) by linking them to the β1-α2 spectrin-based cytoskeleton. Loss of AnkR results in ( 1 ) altered PNN morphology including reduced WFA intensity and decreased compactness of the nets; ( 2 ) molecular changes including reduced β1 spectrin, PNN-associated NrCAM, and Kv3.1b; ( 3 ) behavioral changes including decreased anxiety-like behaviors in the open field and elevated plus maze; and ( 4 ) electrophysiological changes including decreased AP latency and threshold, broader APs with shallower and delayed AHP, and decreased firing rate during current injection.
    Figure Legend Snippet: AnkR is highly expressed in the perisomatic region of Pv + interneurons which are surrounded by PNNs, a specialized ECM structure. AnkR is a scaffolding protein that binds to and stabilizes PNN-associated CAMs (including NrCAM and PlexinA4) and ion channels (including Kv3.1b) by linking them to the β1-α2 spectrin-based cytoskeleton. Loss of AnkR results in ( 1 ) altered PNN morphology including reduced WFA intensity and decreased compactness of the nets; ( 2 ) molecular changes including reduced β1 spectrin, PNN-associated NrCAM, and Kv3.1b; ( 3 ) behavioral changes including decreased anxiety-like behaviors in the open field and elevated plus maze; and ( 4 ) electrophysiological changes including decreased AP latency and threshold, broader APs with shallower and delayed AHP, and decreased firing rate during current injection.

    Techniques Used: Scaffolding, Injection

    7) Product Images from "Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K+ channels"

    Article Title: Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K+ channels

    Journal: bioRxiv

    doi: 10.1101/2021.01.21.427626

    Kv3.1b membrane localization requires AnkR. (A) Immunolabeling of 12-month-old somatosensory cortex from Ank1 F/F and Ank1 F/F ;Dlx5/6-Cre mice using antibodies against AnkR (red) and Kv3.1b (green). Scalebar, 50 μm. (B) Immunoblots of brain homogenates from three 12-month-old control and three 12-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (C) Quantification of Kv3.1b protein normalized to NFM. (D) Immunolabeling of 1-month-old somatosensory cortex from Ank1 F/F mice using antibodies against AnkR (red) and Kv3.3 (green). Scalebar, 25 μm. (E) Immunostaining of Ank1 F/F spinal cord using antibodies against AnkR (red) and Kv3.1b (green). Scalebars, 10 μm and 1 μm. (F) Immunostaining of ventral root nodes of Ranvier in Ank3 +/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkR (red), Kv3.3 (green), and NFasc (blue). Scalebar, 1 μm. (G) The specific K + channels and spectrins found at nodes of Ranvier is dictated by the Ankyrin scaffold to which they bind.
    Figure Legend Snippet: Kv3.1b membrane localization requires AnkR. (A) Immunolabeling of 12-month-old somatosensory cortex from Ank1 F/F and Ank1 F/F ;Dlx5/6-Cre mice using antibodies against AnkR (red) and Kv3.1b (green). Scalebar, 50 μm. (B) Immunoblots of brain homogenates from three 12-month-old control and three 12-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (C) Quantification of Kv3.1b protein normalized to NFM. (D) Immunolabeling of 1-month-old somatosensory cortex from Ank1 F/F mice using antibodies against AnkR (red) and Kv3.3 (green). Scalebar, 25 μm. (E) Immunostaining of Ank1 F/F spinal cord using antibodies against AnkR (red) and Kv3.1b (green). Scalebars, 10 μm and 1 μm. (F) Immunostaining of ventral root nodes of Ranvier in Ank3 +/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkR (red), Kv3.3 (green), and NFasc (blue). Scalebar, 1 μm. (G) The specific K + channels and spectrins found at nodes of Ranvier is dictated by the Ankyrin scaffold to which they bind.

    Techniques Used: Immunolabeling, Mouse Assay, Western Blot, Immunostaining

    AnkR is highly expressed in the somatodendritic domain of Pv + interneurons which are surrounded by PNNs, a specialized ECM structure. AnkR is a scaffolding protein that binds to and stabilizes PNN-associated CAMs (including NrCAM and PlexinA4) and ion channels (including Kv3.1b) by linking them to the β 1- α 2 spectrin-based cytoskeleton. Loss of AnkR results in 1) altered PNN morphology including reduced WFA intensity and decreased compactness of the nets; 2) molecular changes including reduced β 1 spectrin, PNN-associated NrCAM, and Kv3.1b; 3) behavioral changes including decreased anxiety-like behaviors in the open field and elevated plus maze; and 4) electrophysiological changes including decreased AP latency and threshold, broader APs with shallower and delayed AHP, and decreased firing rate during current injection.
    Figure Legend Snippet: AnkR is highly expressed in the somatodendritic domain of Pv + interneurons which are surrounded by PNNs, a specialized ECM structure. AnkR is a scaffolding protein that binds to and stabilizes PNN-associated CAMs (including NrCAM and PlexinA4) and ion channels (including Kv3.1b) by linking them to the β 1- α 2 spectrin-based cytoskeleton. Loss of AnkR results in 1) altered PNN morphology including reduced WFA intensity and decreased compactness of the nets; 2) molecular changes including reduced β 1 spectrin, PNN-associated NrCAM, and Kv3.1b; 3) behavioral changes including decreased anxiety-like behaviors in the open field and elevated plus maze; and 4) electrophysiological changes including decreased AP latency and threshold, broader APs with shallower and delayed AHP, and decreased firing rate during current injection.

    Techniques Used: Scaffolding, Injection

    AnkR binds to Kv3.1b K + channels and is both necessary and sufficient for its membrane localization and clustering. ( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and Kv3.1b (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.
    Figure Legend Snippet: AnkR binds to Kv3.1b K + channels and is both necessary and sufficient for its membrane localization and clustering. ( A, B ) Immunostaining of 1-month-old somatosensory cortex for AnkR (red) and Kv3.1b (green). Low magnification images are shown in ( A ) and high magnification images in ( B ). The genotypes analyzed are shown. Scalebars, 50 µm in ( A ) and 10 µm in ( B ). (C) Immunostaining of human cortical biopsies from two separate patients using antibodies against AnkR (red) and Kv3.1b (green), and DAPI (blue) to label nuclei (Nu). Scalebars, 10 µm. (D) Quantification of Kv3.1b immunofluorescence intensity in control and Ank1 F/F ;Dlx5/6-Cre mice. (E) Immunoblots of brain homogenates from 3 one-month-old control and 3 one-month-old AnkR-deficient brains using antibodies against Kv3.1b, AnkR, and NFM. (F) Quantification of Kv3.1b protein normalized to NFM. ( G, H ). Immunoblots of AnkR-GFP immunoprecipitations in cells co-expressing AnkR-GFP with Myc-tagged β 1 spectrin, full length Flag-tagged Kv3.1b, or truncated versions of Flag-tagged Kv3.1b. The amino acids included in the Flag-tagged Kv3.1b truncation mutants are indicated. (I) The consensus AnkR-binding motif present in Kv3.1b and Kv3.3, but not Kv3.2. (J) Immunoblots of Kv3.1b, AnkR, and Kv2.1 immunoprecipitation reactions using antibodies against AnkR and Kv3.1b. (K) Immunostaining of ventral root nodes of Ranvier in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice using antibodies against AnkG (green), Kv7.2 (red), and neurofascin (NFasc, blue) on the left, and AnkR (green), Kv3.1b (red), and NFasc (blue) on the right. Scalebars, 1μm. (L) Quantification of the percentage of nodes of Ranvier labeled for Kv7.2, Kv3.1b, AnkG, and AnkR in Ank3 F/+ and Ank3 F/F ;ChAT-Cre mice. 60-116 nodes/group. Error bars indicate mean ± SEM.

    Techniques Used: Immunostaining, Immunofluorescence, Mouse Assay, Western Blot, Expressing, Binding Assay, Immunoprecipitation, Labeling

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    Alomone Labs anti kv3 1b
    Expression of <t>Kv3</t> and Kv4 subunits in GAD65-GFP mouse dorsal horn. (A–C) GFP +ve neurones (A) and <t>Kv3.1b</t> subunit immunfluorescence (B) localized to superficial dorsal horn. Merged images (C) indicate a significant overlap between GFP +ve neurones and Kv3.1b immunoreactivity (grey arrows) although examples exist where GFP fluorescence (white arrows, green) and Kv3.1b fluorescence (black arrows, red) are clearly not co-localized. (D–F) GFP +ve neurones (D) and Kv3.3 subunit immunofluorescence (E) localized to superficial dorsal horn. Merged images (C) indicate no significant overlap between GFP +ve neurones (white arrows, green and Kv3.3 immunoreactivity (black arrows, red). (G–I) GFP +ve neurones (G) and Kv4.2 immunoreactivity (H) localized to superficial dorsal horn. Merged images (I) indicate no association between GFP fluorescence (white arrows, green) and immunoreactivity for Kv4.2 (back arrows, red). (J–L) Merged images for GAD 65 +ve neurones (J, green) and Kv 4.3 (K, red) indicate expression by separate populations. Scale bars: A–F, 20 μm; G–L, 50 μm.
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    Expression of Kv3 and Kv4 subunits in GAD65-GFP mouse dorsal horn. (A–C) GFP +ve neurones (A) and Kv3.1b subunit immunfluorescence (B) localized to superficial dorsal horn. Merged images (C) indicate a significant overlap between GFP +ve neurones and Kv3.1b immunoreactivity (grey arrows) although examples exist where GFP fluorescence (white arrows, green) and Kv3.1b fluorescence (black arrows, red) are clearly not co-localized. (D–F) GFP +ve neurones (D) and Kv3.3 subunit immunofluorescence (E) localized to superficial dorsal horn. Merged images (C) indicate no significant overlap between GFP +ve neurones (white arrows, green and Kv3.3 immunoreactivity (black arrows, red). (G–I) GFP +ve neurones (G) and Kv4.2 immunoreactivity (H) localized to superficial dorsal horn. Merged images (I) indicate no association between GFP fluorescence (white arrows, green) and immunoreactivity for Kv4.2 (back arrows, red). (J–L) Merged images for GAD 65 +ve neurones (J, green) and Kv 4.3 (K, red) indicate expression by separate populations. Scale bars: A–F, 20 μm; G–L, 50 μm.

    Journal: Journal of Chemical Neuroanatomy

    Article Title: Kv3.1b and Kv3.3 channel subunit expression in murine spinal dorsal horn GABAergic interneurones

    doi: 10.1016/j.jchemneu.2011.02.003

    Figure Lengend Snippet: Expression of Kv3 and Kv4 subunits in GAD65-GFP mouse dorsal horn. (A–C) GFP +ve neurones (A) and Kv3.1b subunit immunfluorescence (B) localized to superficial dorsal horn. Merged images (C) indicate a significant overlap between GFP +ve neurones and Kv3.1b immunoreactivity (grey arrows) although examples exist where GFP fluorescence (white arrows, green) and Kv3.1b fluorescence (black arrows, red) are clearly not co-localized. (D–F) GFP +ve neurones (D) and Kv3.3 subunit immunofluorescence (E) localized to superficial dorsal horn. Merged images (C) indicate no significant overlap between GFP +ve neurones (white arrows, green and Kv3.3 immunoreactivity (black arrows, red). (G–I) GFP +ve neurones (G) and Kv4.2 immunoreactivity (H) localized to superficial dorsal horn. Merged images (I) indicate no association between GFP fluorescence (white arrows, green) and immunoreactivity for Kv4.2 (back arrows, red). (J–L) Merged images for GAD 65 +ve neurones (J, green) and Kv 4.3 (K, red) indicate expression by separate populations. Scale bars: A–F, 20 μm; G–L, 50 μm.

    Article Snippet: Sections were permeabilised by the inclusion of 0.1% Triton X-100 (Sigma, UK) in the primary antibody solution, washed in PBS (3 × 10 min) and transferred to either anti-Kv3.1b (Alomone Labs, rabbit polyclonal, 1:1000), anti-Kv3.3 (Alomone Labs, rabbit polyclonal, 1:1000), anti-Kv4.2 (NeuroMab, mouse monoclonal, 1:100) or anti-Kv4.3 (NeuroMab, mouse monoclonal, 1:100) primary antibodies for 12–36 h at 4 °C.

    Techniques: Expressing, Fluorescence, Immunofluorescence

    Expression of Kv3 and Kv4 subunits in GAD67-GFP (Δneo) mouse dorsal horn. (A–C) GFP +ve neurones (A) and Kv3.1b subunit immunfluorescence (B) localized to superficial dorsal horn. Merged images (C) indicate that some GFP +ve neurones also express Kv3.1b immunoreactivity (grey arrows) although examples exist where GFP fluorescence (white arrows, green) and Kv3.1b fluorescence (black arrows, red) are not co-localized. (D–F) GFP +ve neurones (D) and Kv3.3 subunit immunofluorescence (E) localized to superficial dorsal horn. Merged images (C) indicate no significant overlap between GFP +ve neurones (white arrows, green and Kv3.3 immunoreactivity (black arrows, red). (G–H) GFP +ve neurones (G) and Kv4.2 immunoreactivity (H) localized to superficial dorsal horn. (J–K) GFP +ve neurones (J) and Kv4.2 immunoreactivity (K) localized to superficial dorsal horn. Merged images (I and L) indicate no association between GFP fluorescence (white arrows, green) and immunoreactivity for Kv4.2 or Kv4.3 (black arrows, red). Scale bars all 20 μm.

    Journal: Journal of Chemical Neuroanatomy

    Article Title: Kv3.1b and Kv3.3 channel subunit expression in murine spinal dorsal horn GABAergic interneurones

    doi: 10.1016/j.jchemneu.2011.02.003

    Figure Lengend Snippet: Expression of Kv3 and Kv4 subunits in GAD67-GFP (Δneo) mouse dorsal horn. (A–C) GFP +ve neurones (A) and Kv3.1b subunit immunfluorescence (B) localized to superficial dorsal horn. Merged images (C) indicate that some GFP +ve neurones also express Kv3.1b immunoreactivity (grey arrows) although examples exist where GFP fluorescence (white arrows, green) and Kv3.1b fluorescence (black arrows, red) are not co-localized. (D–F) GFP +ve neurones (D) and Kv3.3 subunit immunofluorescence (E) localized to superficial dorsal horn. Merged images (C) indicate no significant overlap between GFP +ve neurones (white arrows, green and Kv3.3 immunoreactivity (black arrows, red). (G–H) GFP +ve neurones (G) and Kv4.2 immunoreactivity (H) localized to superficial dorsal horn. (J–K) GFP +ve neurones (J) and Kv4.2 immunoreactivity (K) localized to superficial dorsal horn. Merged images (I and L) indicate no association between GFP fluorescence (white arrows, green) and immunoreactivity for Kv4.2 or Kv4.3 (black arrows, red). Scale bars all 20 μm.

    Article Snippet: Sections were permeabilised by the inclusion of 0.1% Triton X-100 (Sigma, UK) in the primary antibody solution, washed in PBS (3 × 10 min) and transferred to either anti-Kv3.1b (Alomone Labs, rabbit polyclonal, 1:1000), anti-Kv3.3 (Alomone Labs, rabbit polyclonal, 1:1000), anti-Kv4.2 (NeuroMab, mouse monoclonal, 1:100) or anti-Kv4.3 (NeuroMab, mouse monoclonal, 1:100) primary antibodies for 12–36 h at 4 °C.

    Techniques: Expressing, Fluorescence, Immunofluorescence

    Distribution and interaction with ankyrin G of Kv3.1b and Kv3.2 subunits. A, B , Membrane proteins (100 μg) from Kv3.1-deficient mouse brain and rat brain, spinal cord, optic nerve, and sciatic nerve were fractioned in SDS gel and immunoblotted for Kv3.1b ( A ) and Kv3.2 ( B ). Kv3.1b was expressed in rat brain and spinal cord, and to a lesser extents in the optic nerve. No band was observed in Kv3.1-deficient mouse. Kv3.2 was detected in the mouse and rat brain and to a lesser extent in the rat spinal cord. C , Brain and spinal cord membranes (200 μg) were immunoprecipitated separately for Kv3.1b or Kv3.2 and then immunoblotted for Kv3.1b. Note that Kv3.1b coimmunoprecipitates extensively with Kv3.2 in the brain but not in the spinal cord. D, E , Brain and spinal cord membranes (200 μg) were immunoprecipitated with rabbit anti-ankyrin G (Ank-G), rabbit anti-Kv3.1b, rabbit anti-Kv3.2, or mouse anti-Kv1.2 antibodies, then immunoblotted for Kv3.1b ( D ) or ankyrin G ( E ). Brain membrane (BM) was used as a positive control. Kv3.1b coimmunoprecipitates with ankyrin G and Kv3.2, but not Kv1.2 ( D ). A low-molecular-weight isoform of ankyrin G was pulled down with Kv3.1b, but not with either Kv3.2 or Kv1.2 ( E ). Molecular weight markers are shown on the right in kilodaltons.

    Journal: The Journal of Neuroscience

    Article Title: Kv3.1b Is a Novel Component of CNS Nodes

    doi: 10.1523/JNEUROSCI.23-11-04509.2003

    Figure Lengend Snippet: Distribution and interaction with ankyrin G of Kv3.1b and Kv3.2 subunits. A, B , Membrane proteins (100 μg) from Kv3.1-deficient mouse brain and rat brain, spinal cord, optic nerve, and sciatic nerve were fractioned in SDS gel and immunoblotted for Kv3.1b ( A ) and Kv3.2 ( B ). Kv3.1b was expressed in rat brain and spinal cord, and to a lesser extents in the optic nerve. No band was observed in Kv3.1-deficient mouse. Kv3.2 was detected in the mouse and rat brain and to a lesser extent in the rat spinal cord. C , Brain and spinal cord membranes (200 μg) were immunoprecipitated separately for Kv3.1b or Kv3.2 and then immunoblotted for Kv3.1b. Note that Kv3.1b coimmunoprecipitates extensively with Kv3.2 in the brain but not in the spinal cord. D, E , Brain and spinal cord membranes (200 μg) were immunoprecipitated with rabbit anti-ankyrin G (Ank-G), rabbit anti-Kv3.1b, rabbit anti-Kv3.2, or mouse anti-Kv1.2 antibodies, then immunoblotted for Kv3.1b ( D ) or ankyrin G ( E ). Brain membrane (BM) was used as a positive control. Kv3.1b coimmunoprecipitates with ankyrin G and Kv3.2, but not Kv1.2 ( D ). A low-molecular-weight isoform of ankyrin G was pulled down with Kv3.1b, but not with either Kv3.2 or Kv1.2 ( E ). Molecular weight markers are shown on the right in kilodaltons.

    Article Snippet: Rabbit antisera against Kv3.1b or Kv3.2 (#APC-014, diluted 1:100; Alomone Labs, Jerusalem, Israel), and mouse monoclonal antibodies raised against Kv1.2 (diluted 1:100; Upstate Biotechnology, Lake Placid, NY), MAG (513, diluted 1:100; Boehringer Mannheim, Indianapolis, IN), and PanNav channels (diluted 1:50; Sigma, St. Louis, MO) were used.

    Techniques: SDS-Gel, Immunoprecipitation, Positive Control, Molecular Weight

    Ankyrin G differentially regulates polarized targeting of Kv3.1 splice variants. A – G , Hippocampal neurons at 7 DIV were cotransfected with ankyrin G and Kv3.1 constructs, fixed, and stained with anti-HA antibodies under permeabilized conditions. A , Kv3.1aHA (red) did not colocalize with ankyrin G-GFP (green) in axons. B , Kv3.1bHA (red) colocalized with ankyrin G-GFP (green) in p roximal axons. C , The fluorescence intensity profiles along the axons in A and B indicated by arrows. Arrowheads, The fluorescence peaks attributable to crossing dendrites. D , E , The levels of Kv3.1aHA ( D ) and Kv3.1bHA ( E ) in axons markedly increased and modestly decreased, respectively, in the presence of MB–GFP (green). F , The fluorescence intensity profiles along the axons in D and E indicated by arrows. G , Summary of polarized targeting of Kv3.1aHA and Kv3.1bHA in the presence of GFP and MB–GFP. F axon / F dendrite is average fluorescence intensity in axons/average fluorescence intensity in dendrites. H , Endogenous Kv3.1b channels were localized to distal axons (arrows), in contrast to the localization of endogenous Nav channels in proximal axons. Hippocampal neurons at 30 DIV were costained with polyclonal anti-Kv3.1b (green) and monoclonal anti-pan-Nav channel (red) antibodies. It is important to note that Kv3.1b also localizes in soma and proximal dendrites. I , Fluorescence intensity profiles along the axon indicated by arrows in H . Distance 0, Soma. Scale bars, 100 μm. Student's t test used in the comparison of two groups. * p

    Journal: The Journal of Neuroscience

    Article Title: The Axon–Dendrite Targeting of Kv3 (Shaw) Channels Is Determined by a Targeting Motif That Associates with the T1 Domain and Ankyrin G

    doi: 10.1523/JNEUROSCI.3675-07.2007

    Figure Lengend Snippet: Ankyrin G differentially regulates polarized targeting of Kv3.1 splice variants. A – G , Hippocampal neurons at 7 DIV were cotransfected with ankyrin G and Kv3.1 constructs, fixed, and stained with anti-HA antibodies under permeabilized conditions. A , Kv3.1aHA (red) did not colocalize with ankyrin G-GFP (green) in axons. B , Kv3.1bHA (red) colocalized with ankyrin G-GFP (green) in p roximal axons. C , The fluorescence intensity profiles along the axons in A and B indicated by arrows. Arrowheads, The fluorescence peaks attributable to crossing dendrites. D , E , The levels of Kv3.1aHA ( D ) and Kv3.1bHA ( E ) in axons markedly increased and modestly decreased, respectively, in the presence of MB–GFP (green). F , The fluorescence intensity profiles along the axons in D and E indicated by arrows. G , Summary of polarized targeting of Kv3.1aHA and Kv3.1bHA in the presence of GFP and MB–GFP. F axon / F dendrite is average fluorescence intensity in axons/average fluorescence intensity in dendrites. H , Endogenous Kv3.1b channels were localized to distal axons (arrows), in contrast to the localization of endogenous Nav channels in proximal axons. Hippocampal neurons at 30 DIV were costained with polyclonal anti-Kv3.1b (green) and monoclonal anti-pan-Nav channel (red) antibodies. It is important to note that Kv3.1b also localizes in soma and proximal dendrites. I , Fluorescence intensity profiles along the axon indicated by arrows in H . Distance 0, Soma. Scale bars, 100 μm. Student's t test used in the comparison of two groups. * p

    Article Snippet: The following reagents were used: rabbit polyclonal anti-MAP2 (Chemicon, Temecula, CA), rat monoclonal anti-HA antibody (Roche, Indianapolis, IN), mouse monoclonal anti-human CD4 antibody (Caltag, Burlingame, CA), mouse monoclonal anti-pan-Nav channel antibody (Sigma, St. Louis, MO), rabbit polyclonal anti-Kv3.1b antibody (Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-EGFP and anti-6×His antibodies (Invitrogen, Carlsbad, CA), rabbit polyclonal anti-ankyrin G (a kind gift from Dr. Vann Bennett) and anti-Tau1 (Abcam, Cambridge, MA), Cy2-, Cy5- and HRP-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA), Kv3.1a and Kv3.1b (a kind gift from Dr. Bernardo Rudy), transferrin receptor-GFP fusion (TfR–GFP, a kind gift from Dr. Gary Banker), ankyrin G-GFP (a kind gift from Drs. Lori Isom and Vann Bennett).

    Techniques: Construct, Staining, Fluorescence

    Math5-lacZ is coexpressed with bushy cell markers in the ventral cochlear nucleus A-H. Cryosections through the VCN of M5 +/− mice (panels A, C, E, G) and M5 −/− mice (panels B, D, F, H) were immunostained for β-galactosidase (red) neuronal antigens (green). A-D. The Kv1.1 and Kv3.1b potassium channels are expressed by all Math5-lacZ cells (arrowheads) and a roughly equal number of lacZ -negative cells (arrows). The co-labeled cells represent a subset of globular and spherical bushy cells within the VCN. E, F. The Kcnq4 channel is similarly expressed by all Math5-lacZ positive cells (arrowhead) and a larger number of lacZ -negative cells (arrow). G, H. Calbindin is expressed by a subset of Math5-lacZ positive cells in the VCN (arrowhead). Additional cells are positive for calbindin only (open arrow) or β-gal only (closed arrow). This indicates that the Math5 -expressing cell population is heterogeneous. Abbreviations: VCN, ventral cochlear nucleus. Scale bar, 100 μm.

    Journal: Molecular and cellular neurosciences

    Article Title: Math5 expression and function in the central auditory system

    doi: 10.1016/j.mcn.2007.09.006

    Figure Lengend Snippet: Math5-lacZ is coexpressed with bushy cell markers in the ventral cochlear nucleus A-H. Cryosections through the VCN of M5 +/− mice (panels A, C, E, G) and M5 −/− mice (panels B, D, F, H) were immunostained for β-galactosidase (red) neuronal antigens (green). A-D. The Kv1.1 and Kv3.1b potassium channels are expressed by all Math5-lacZ cells (arrowheads) and a roughly equal number of lacZ -negative cells (arrows). The co-labeled cells represent a subset of globular and spherical bushy cells within the VCN. E, F. The Kcnq4 channel is similarly expressed by all Math5-lacZ positive cells (arrowhead) and a larger number of lacZ -negative cells (arrow). G, H. Calbindin is expressed by a subset of Math5-lacZ positive cells in the VCN (arrowhead). Additional cells are positive for calbindin only (open arrow) or β-gal only (closed arrow). This indicates that the Math5 -expressing cell population is heterogeneous. Abbreviations: VCN, ventral cochlear nucleus. Scale bar, 100 μm.

    Article Snippet: Primary rabbit antibodies were: anti-Kcnq4 (gift of Thomas Jentsch, University of Hamburg, Germany, 1:400), anti-Kv1.1 (Alomone Labs, Jerusalem, Israel, 1:300), anti-Kv3.1b (Alomone Labs, 1:750), anti-Math1 (gift of Jane Johnson, University of Texas Southwestern, Dallas, TX, 1:250) and anti-β-galactosidase (Cappell, 1:5000).

    Techniques: Mouse Assay, Labeling, Expressing