girk1  (Alomone Labs)


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    Structured Review

    Alomone Labs girk1
    Functional comparison of GIRK2a and GIRK2c in HEK cells. ( A ) Whole-cell currents (V hold = −70 mV) evoked by baclofen (100 μM) in HEK cells expressing GABA B R, <t>GIRK1,</t> and either GIRK2a (red) or GIRK2c (blue). No current was evoked by baclofen in cells expressing only GABA B R (control, black). Scale: 500 pA/10 s. ( B ) Summary of baclofen-induced, steady-state current densities (I baclofen , pA/pF) in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c ( t 19 = 1.1, P = 0.29; n = 10–11/group). Individual data points are represented as small squares overlapping the relevant bar in the plot. ( C,D ) Activation ( t 19 = 1.5, P = 0.16) and deactivation ( t 16 = 0.4, P = 0.72) kinetics for baclofen-induced currents in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c (n = 9–11/group). ( E ) Representative concentration-response experiment for a HEK cell expressing GABA B R and GIRK1/GIRK2c. Scale: 500 pA/10 s. ( F,G ) Summary of concentration-response experiments for baclofen-induced currents in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c ( t 17 = 1.0, P = 0.32; n = 9–10/group). Currents were normalized to the response evoked by 100 μM baclofen in each experiment.
    Girk1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/girk1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    girk1 - by Bioz Stars, 2022-07
    94/100 stars

    Images

    1) Product Images from "GIRK2 splice variants and neuronal G protein-gated K+ channels: implications for channel function and behavior"

    Article Title: GIRK2 splice variants and neuronal G protein-gated K+ channels: implications for channel function and behavior

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-01820-2

    Functional comparison of GIRK2a and GIRK2c in HEK cells. ( A ) Whole-cell currents (V hold = −70 mV) evoked by baclofen (100 μM) in HEK cells expressing GABA B R, GIRK1, and either GIRK2a (red) or GIRK2c (blue). No current was evoked by baclofen in cells expressing only GABA B R (control, black). Scale: 500 pA/10 s. ( B ) Summary of baclofen-induced, steady-state current densities (I baclofen , pA/pF) in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c ( t 19 = 1.1, P = 0.29; n = 10–11/group). Individual data points are represented as small squares overlapping the relevant bar in the plot. ( C,D ) Activation ( t 19 = 1.5, P = 0.16) and deactivation ( t 16 = 0.4, P = 0.72) kinetics for baclofen-induced currents in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c (n = 9–11/group). ( E ) Representative concentration-response experiment for a HEK cell expressing GABA B R and GIRK1/GIRK2c. Scale: 500 pA/10 s. ( F,G ) Summary of concentration-response experiments for baclofen-induced currents in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c ( t 17 = 1.0, P = 0.32; n = 9–10/group). Currents were normalized to the response evoked by 100 μM baclofen in each experiment.
    Figure Legend Snippet: Functional comparison of GIRK2a and GIRK2c in HEK cells. ( A ) Whole-cell currents (V hold = −70 mV) evoked by baclofen (100 μM) in HEK cells expressing GABA B R, GIRK1, and either GIRK2a (red) or GIRK2c (blue). No current was evoked by baclofen in cells expressing only GABA B R (control, black). Scale: 500 pA/10 s. ( B ) Summary of baclofen-induced, steady-state current densities (I baclofen , pA/pF) in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c ( t 19 = 1.1, P = 0.29; n = 10–11/group). Individual data points are represented as small squares overlapping the relevant bar in the plot. ( C,D ) Activation ( t 19 = 1.5, P = 0.16) and deactivation ( t 16 = 0.4, P = 0.72) kinetics for baclofen-induced currents in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c (n = 9–11/group). ( E ) Representative concentration-response experiment for a HEK cell expressing GABA B R and GIRK1/GIRK2c. Scale: 500 pA/10 s. ( F,G ) Summary of concentration-response experiments for baclofen-induced currents in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c ( t 17 = 1.0, P = 0.32; n = 9–10/group). Currents were normalized to the response evoked by 100 μM baclofen in each experiment.

    Techniques Used: Functional Assay, Expressing, Activation Assay, Concentration Assay

    2) Product Images from "Activation and inhibition of G protein-coupled inwardly rectifying potassium (Kir3) channels by G protein ?? subunits"

    Article Title: Activation and inhibition of G protein-coupled inwardly rectifying potassium (Kir3) channels by G protein ?? subunits

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    Gβ5-containing dimers inhibit basal GIRK1,4 currents and bind to GIRK1,4 channel cytoplasmic domains. ( A ) Sample current traces from control cells and G1,4 cells expressing either β1γ2 or β5γ2. Inwardly rectifying current was enhanced by β1γ2 but inhibited by β5γ2. ( Inset ) Conductance (±SEM) is decreased in cells transfected with β5γ2 or β5γ11. *, Statistically significant differences from control. *, Significantly different from control ( P
    Figure Legend Snippet: Gβ5-containing dimers inhibit basal GIRK1,4 currents and bind to GIRK1,4 channel cytoplasmic domains. ( A ) Sample current traces from control cells and G1,4 cells expressing either β1γ2 or β5γ2. Inwardly rectifying current was enhanced by β1γ2 but inhibited by β5γ2. ( Inset ) Conductance (±SEM) is decreased in cells transfected with β5γ2 or β5γ11. *, Statistically significant differences from control. *, Significantly different from control ( P

    Techniques Used: Expressing, Transfection

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    Alomone Labs anti girk1
    Confirmation of NIP–β2 subunit interactions. ( A ) Representative blots showing immunoreactivity to dynamin 1, <t>GIRK1,</t> and G oα subunits within the immunoprecipitated complexes from the brain of WT (+/+) and β2 −/−
    Anti Girk1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Confirmation of NIP–β2 subunit interactions. ( A ) Representative blots showing immunoreactivity to dynamin 1, GIRK1, and G oα subunits within the immunoprecipitated complexes from the brain of WT (+/+) and β2 −/−

    Journal:

    Article Title: Intracellular complexes of the ?2 subunit of the nicotinic acetylcholine receptor in brain identified by proteomics

    doi: 10.1073/pnas.0710314104

    Figure Lengend Snippet: Confirmation of NIP–β2 subunit interactions. ( A ) Representative blots showing immunoreactivity to dynamin 1, GIRK1, and G oα subunits within the immunoprecipitated complexes from the brain of WT (+/+) and β2 −/−

    Article Snippet: Western blot analysis was performed with the following primary antibodies: anti-β2 (H-92) (Santa Cruz Biotechnology); anti-α4 (H-133) (Santa Cruz Biotechnology); anti-clathrin HC (Santa Cruz Biotechnology); anti-NSF (Upstate Biotechnology); anti-dynamin 1 (PharMingen–Becton Dickinson); anti-synaptotagmin 1 (Chemicon); anti-GTP-binding protein (G protein) Goα (Santa Cruz Biotechnology), anti-GIRK1 (Alomone Laboratories); and anti-GST (Amersham Pharmacia).

    Techniques: Immunoprecipitation

    Gα i3 GA regulates GIRK1* in excised plasma membrane patches

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: Gα i3 GA regulates GIRK1* in excised plasma membrane patches

    Article Snippet: Blocking of non-specific binding sites was done with donkey immunoglobulin G (IgG, whole molecule, 1/400, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 30 min. Each coverslip was incubated for 1 h with antibodies against GIRK1 or GIRK2 (Alomone Labs, Jerusalem, Israel).

    Techniques:

    Gα i3 regulates GIRK1* activation in whole oocytes

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: Gα i3 regulates GIRK1* activation in whole oocytes

    Article Snippet: Blocking of non-specific binding sites was done with donkey immunoglobulin G (IgG, whole molecule, 1/400, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 30 min. Each coverslip was incubated for 1 h with antibodies against GIRK1 or GIRK2 (Alomone Labs, Jerusalem, Israel).

    Techniques: Activation Assay

    Regulation of GIRK1/2 channels expressed in HEK293 cells

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: Regulation of GIRK1/2 channels expressed in HEK293 cells

    Article Snippet: Blocking of non-specific binding sites was done with donkey immunoglobulin G (IgG, whole molecule, 1/400, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 30 min. Each coverslip was incubated for 1 h with antibodies against GIRK1 or GIRK2 (Alomone Labs, Jerusalem, Israel).

    Techniques:

    The C terminus of GIRK1 is important for Gα i modulation

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: The C terminus of GIRK1 is important for Gα i modulation

    Article Snippet: Blocking of non-specific binding sites was done with donkey immunoglobulin G (IgG, whole molecule, 1/400, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 30 min. Each coverslip was incubated for 1 h with antibodies against GIRK1 or GIRK2 (Alomone Labs, Jerusalem, Israel).

    Techniques:

    Functional differences between homomeric GIRK1 and GIRK2

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: Functional differences between homomeric GIRK1 and GIRK2

    Article Snippet: Blocking of non-specific binding sites was done with donkey immunoglobulin G (IgG, whole molecule, 1/400, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 30 min. Each coverslip was incubated for 1 h with antibodies against GIRK1 or GIRK2 (Alomone Labs, Jerusalem, Israel).

    Techniques: Functional Assay

    Gα i3 GA improves GIRK1/2 activation by added Gβγ protein in excised plasma membrane patches

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: Gα i3 GA improves GIRK1/2 activation by added Gβγ protein in excised plasma membrane patches

    Article Snippet: Blocking of non-specific binding sites was done with donkey immunoglobulin G (IgG, whole molecule, 1/400, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 30 min. Each coverslip was incubated for 1 h with antibodies against GIRK1 or GIRK2 (Alomone Labs, Jerusalem, Israel).

    Techniques: Activation Assay

    Biochemical and functional differences between GIRK1 and GIRK2

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: Biochemical and functional differences between GIRK1 and GIRK2

    Article Snippet: Blocking of non-specific binding sites was done with donkey immunoglobulin G (IgG, whole molecule, 1/400, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 30 min. Each coverslip was incubated for 1 h with antibodies against GIRK1 or GIRK2 (Alomone Labs, Jerusalem, Israel).

    Techniques: Functional Assay

    The basal activity is Gβγ dependent in GIRK1*, but Gβγ independent in GIRK2

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: The basal activity is Gβγ dependent in GIRK1*, but Gβγ independent in GIRK2

    Article Snippet: Blocking of non-specific binding sites was done with donkey immunoglobulin G (IgG, whole molecule, 1/400, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 30 min. Each coverslip was incubated for 1 h with antibodies against GIRK1 or GIRK2 (Alomone Labs, Jerusalem, Israel).

    Techniques: Activity Assay

    Gα i3 GA improves GIRK1/2 activation by coexpressed Gβγ in whole oocytes

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: Gα i3 GA improves GIRK1/2 activation by coexpressed Gβγ in whole oocytes

    Article Snippet: Blocking of non-specific binding sites was done with donkey immunoglobulin G (IgG, whole molecule, 1/400, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 30 min. Each coverslip was incubated for 1 h with antibodies against GIRK1 or GIRK2 (Alomone Labs, Jerusalem, Israel).

    Techniques: Activation Assay

    Functional comparison of GIRK2a and GIRK2c in HEK cells. ( A ) Whole-cell currents (V hold = −70 mV) evoked by baclofen (100 μM) in HEK cells expressing GABA B R, GIRK1, and either GIRK2a (red) or GIRK2c (blue). No current was evoked by baclofen in cells expressing only GABA B R (control, black). Scale: 500 pA/10 s. ( B ) Summary of baclofen-induced, steady-state current densities (I baclofen , pA/pF) in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c ( t 19 = 1.1, P = 0.29; n = 10–11/group). Individual data points are represented as small squares overlapping the relevant bar in the plot. ( C,D ) Activation ( t 19 = 1.5, P = 0.16) and deactivation ( t 16 = 0.4, P = 0.72) kinetics for baclofen-induced currents in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c (n = 9–11/group). ( E ) Representative concentration-response experiment for a HEK cell expressing GABA B R and GIRK1/GIRK2c. Scale: 500 pA/10 s. ( F,G ) Summary of concentration-response experiments for baclofen-induced currents in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c ( t 17 = 1.0, P = 0.32; n = 9–10/group). Currents were normalized to the response evoked by 100 μM baclofen in each experiment.

    Journal: Scientific Reports

    Article Title: GIRK2 splice variants and neuronal G protein-gated K+ channels: implications for channel function and behavior

    doi: 10.1038/s41598-017-01820-2

    Figure Lengend Snippet: Functional comparison of GIRK2a and GIRK2c in HEK cells. ( A ) Whole-cell currents (V hold = −70 mV) evoked by baclofen (100 μM) in HEK cells expressing GABA B R, GIRK1, and either GIRK2a (red) or GIRK2c (blue). No current was evoked by baclofen in cells expressing only GABA B R (control, black). Scale: 500 pA/10 s. ( B ) Summary of baclofen-induced, steady-state current densities (I baclofen , pA/pF) in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c ( t 19 = 1.1, P = 0.29; n = 10–11/group). Individual data points are represented as small squares overlapping the relevant bar in the plot. ( C,D ) Activation ( t 19 = 1.5, P = 0.16) and deactivation ( t 16 = 0.4, P = 0.72) kinetics for baclofen-induced currents in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c (n = 9–11/group). ( E ) Representative concentration-response experiment for a HEK cell expressing GABA B R and GIRK1/GIRK2c. Scale: 500 pA/10 s. ( F,G ) Summary of concentration-response experiments for baclofen-induced currents in HEK cells expressing GIRK1/GIRK2a or GIRK1/GIRK2c ( t 17 = 1.0, P = 0.32; n = 9–10/group). Currents were normalized to the response evoked by 100 μM baclofen in each experiment.

    Article Snippet: Membranes were blocked in 5% milk/PBS, incubated overnight at 4 °C with rabbit polyclonal antibodies directed against GIRK1 (1:200, Alomone Labs; Jerusalem, Israel), GIRK2 (1:200, Alomone Labs), GIRK3 (1 μg/mL, Frontier Institute Co.; Ishikari, Japan), or β-actin (0.27 μg/mL, Abcam; Cambridge, MA), and diluted in 5% milk/PBS/0.1% Tween 20.

    Techniques: Functional Assay, Expressing, Activation Assay, Concentration Assay

    GIRK1 expression in IB4-binding peptidergic TG neurons

    Journal: European journal of pain (London, England)

    Article Title: Peripheral G protein-coupled inwardly rectifying potassium (GIRK) channels are involved in delta opioid receptor-mediated anti-hyperalgesia in rat masseter muscle

    doi: 10.1002/j.1532-2149.2013.00343.x

    Figure Lengend Snippet: GIRK1 expression in IB4-binding peptidergic TG neurons

    Article Snippet: Each membrane was incubated with GIRK1 antibody (1:200; rabbit polyclonal; Alomone labs), GIRK2 antibody (1:200; rabbit polyclonal; Santa Cruz), respectively.

    Techniques: Expressing, Binding Assay