trypsin (Worthington Biochemical)

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Name:

Trypsin

Description:

Supplied as dialyzed and lyophilized powder

Catalog Number:

LS003702

Price:

Source:

Bovine Pancreas

Size:

100 mg

Cas Number:

9002.07.7

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Structured Review

Worthington Biochemical trypsin
Supplied as dialyzed and lyophilized powder
https://www.bioz.com/result/trypsin/product/Worthington Biochemical
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99

trypsin - by Bioz Stars, 2021-10

97/100 stars

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Article Title: Long-term culture of SH-SY5Y neuroblastoma cells in the absence of neurotrophins: A novel model of neuronal ageing
Article Snippet: Cells were trypsinised in 0.05% trypsin (Worthington’s, USA) in calcium and magnesium free buffer solution, and plated on either 96 well culture plates (10,000 cells per well; Nunc, vwr, UK) or on 0.0005% w/v poly-L -lysine-coated 13 mm diameter borosilicate glass coverslips (50,000 cells per coverslip), in DMEM supplemented with 4500 mg/L D-glucose, 10 mg/ml penicillin/ streptomycin and 10% iron-fortified new-born calf serum.

Article Title: Examining the Effects of an Anti-Salmonella Bacteriophage Preparation, BAFASAL®, on Ex-Vivo Human Gut Microbiome Composition and Function Using a Multi-Omics Approach
Article Snippet: For each sample, 50 μg of protein was reduced with 10 mM dithiothreitol (DTT) at 56 °C for 30 min and alkylated in the dark with 20 mM iodoacetamide (IAA) at room temperature for 45 min, followed by 10× dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37 °C for 20 h with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ, USA) per 50 µg protein.

Article Title: Molecular Evolution across Mouse Spermatogenesis
Article Snippet: Briefly, we decapsulated testes and washed them with twice with 1mg/mL collagenase (Worthington Biochemical), 0.004mg/mL DNase I (Qiagen), and GBSS (Sigma), followed by disassociation with 1mg/mL trypsin (Worthington Biochemical) and 0.004mg/mL DNase I.

Article Title: Identification of bone morphogenetic protein 4 in the saliva after the placement of fixed orthodontic appliance
Article Snippet: Pooled protein samples (40 μg) were alkylated (55 mM iodoacetamide in 8 M urea) and digested with 0.8 μg trypsin (Worthington, TPCK treated) in 10-kDa centrifugal filter units.

Incubation:

Article Title: Posttranslational Regulation of Botulinum Neurotoxin Production in Clostridium botulinum Hall A-hyper
Article Snippet: .. In short, culture supernatant samples were treated with 5 μg/ml of trypsin (Worthington Biochemical Corporation) and incubated at 37°C for 1 h to proteolytically activate the BoNT. ..

Passaging:

Article Title: Differential Regulation of Circulating Soluble Receptor for Advanced Glycation End Products (sRAGEs) and Its Ligands S100A8/A9 Four Weeks Post an Exercise Intervention in a Cohort of Young Army Recruits
Article Snippet: .. Cells were harvested for passaging at 2 wk intervals with a trypsin- (0.01%, VMF, Worthington Biochemical Corp., Freehold, NJ, USA) EDTA (0.02%, Sigma, St. Louis, MO, USA) solution and plated. ..

Infection:

Article Title: High expression of CD38 and MHC class II on CD8+ T cells during severe influenza disease reflects bystander activation and trogocytosis
Article Snippet: .. Cells were infected with lung homogenate supernatants at varying dilutions for 45 min at 37°C before the addition of Leibovitz L15 or MEM medium containing 0.9% agarose overlay containing Trypsin (Worthington Biochemical, NJ, USA), as previously described. ..

Staining:

Article Title: Pneumococcal surface adhesion A protein (PsaA) interacts with human Annexin A2 on airway epithelial cells
Article Snippet: .. Corresponding spots from Coomassie stained gel were excised and digested with trypsin (Worthington). ..