trypsin  (Worthington Biochemical)


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  • 97
    Name:
    Trypsin
    Description:
    Supplied as dialyzed and lyophilized powder
    Catalog Number:
    LS003702
    Price:
    23
    Source:
    Bovine Pancreas
    Size:
    100 mg
    Cas Number:
    9002.07.7
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    Structured Review

    Worthington Biochemical trypsin
    Supplied as dialyzed and lyophilized powder
    https://www.bioz.com/result/trypsin/product/Worthington Biochemical
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsin - by Bioz Stars, 2021-07
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication
    Article Snippet: .. A/WSN/33 (WSN, H1N1) and A/Fuzhou/1/2009 (FZ09, H1N1) were propagated in MDCK cells cultured in MEM containing 0.3% bovine serum albumin (BSA, Sigma-Aldrich) and 0.5 μg/ml L-1-tosylamide-2-phenylmethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington, Lakewood, NJ). .. All experiments with H5N1 and H7N9 viruses were conducted within the enhanced animal biosafety level 3 (ABSL3+) facility in the Harbin Veterinary Research Institute (HVRI) of the Chinese Academy of Agricultural Sciences (CAAS), which is approved for such use by the Ministry of Agriculture of China and the China National Accreditation Service for Conformity Assessment.

    Staining:

    Article Title: N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity
    Article Snippet: SDS-PAGE and NanoLC-MS/MS AnalysisFor sample clean-up, a short SDS-PAGE run (NuPAGE™ 4–12% Bis-Tris Protein Gels, Thermo Fisher Scientific) of the samples obtained from the MGL pull-downs after PNGase F treatment was performed. .. Gels were stained with SimplyBlue™ Safe Stain (Invitrogen) for 1 h at room temperature (RT) and washed with distilled water for 3 h. Bands corresponding to the whole lane were cut from the gel, and the proteins were then subjected to reduction with dithiothreitol (10 mM), alkylation with iodoacetamide (50 mM) and in-gel trypsin digestion with trypsin (Worthington Enzymes Lakewood, NJ, United States), using a Proteineer DP digestion robot (Bruker, Billerica, MA, United States) [ ]. .. Tryptic peptides were separated by online C18 nano- High Performance Liquid Chromatography (HPLC)-MS/MS with an Easy nLC 1000 gradient HPLC system (Thermo, Bremen, Germany) coupled to an Orbitrap Fusion LUMOS mass spectrometer (Thermo), as previously described [ ].

    Isolation:

    Article Title: Transplantation of CD15-Enriched Murine Neural Stem Cells Increases Total Engraftment and Shifts Differentiation Toward the Oligodendrocyte Lineage
    Article Snippet: .. NSC cultures were isolated as previously described [ ] with the following modifications: (a) digestion of tissue in 0.25% trypsin (Worthington Biochemical, Lakewood, NJ, ) was performed, and (b) Dulbecco's Modified Eagle's Medium: Nutrient Mixture F-12 (DMEM/F12; 1:1 ratio; Gibco-BRL, Gaithersburg, MD, ) culture medium was used. ..

    Modification:

    Article Title: Transplantation of CD15-Enriched Murine Neural Stem Cells Increases Total Engraftment and Shifts Differentiation Toward the Oligodendrocyte Lineage
    Article Snippet: .. NSC cultures were isolated as previously described [ ] with the following modifications: (a) digestion of tissue in 0.25% trypsin (Worthington Biochemical, Lakewood, NJ, ) was performed, and (b) Dulbecco's Modified Eagle's Medium: Nutrient Mixture F-12 (DMEM/F12; 1:1 ratio; Gibco-BRL, Gaithersburg, MD, ) culture medium was used. ..

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