glua2  (Alomone Labs)


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    Name:
    Anti CACNA2D1 Cavalpha2delta1 extracellular Antibody
    Description:
    Anti CACNA2D1 Cavalpha2delta1 extracellular Antibody is directed against an epitope of rabbit CaVα2δ1 Anti CACNA2D1 CaVα2δ1 extracellular Antibody ACC 015 can be used in western blot and live cell imaging applications It is especially suited to detect CaVα2δ1 in live cells It has been designed to recognize CaVα2δ1 from rat mouse and human samples
    Catalog Number:
    ACC-015
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs glua2
    Anti CACNA2D1 Cavalpha2delta1 extracellular Antibody
    Anti CACNA2D1 Cavalpha2delta1 extracellular Antibody is directed against an epitope of rabbit CaVα2δ1 Anti CACNA2D1 CaVα2δ1 extracellular Antibody ACC 015 can be used in western blot and live cell imaging applications It is especially suited to detect CaVα2δ1 in live cells It has been designed to recognize CaVα2δ1 from rat mouse and human samples
    https://www.bioz.com/result/glua2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glua2 - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly"

    Article Title: α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly

    Journal: Cell reports

    doi: 10.1016/j.celrep.2021.109396

    α2δ-1 reduces surface and synaptic expression of GluA2 and heteromeric GluA1/GluA2 receptors (A) Original blots and quantification show the protein levels of GluA1 and GluA2 in HEK293 cells cotransfected with GluA1/GluA2 and control vector (pcDNA3, P3), α2δ-1, α2δ-2, or α2δ-3 (n = 4 per group). ***p
    Figure Legend Snippet: α2δ-1 reduces surface and synaptic expression of GluA2 and heteromeric GluA1/GluA2 receptors (A) Original blots and quantification show the protein levels of GluA1 and GluA2 in HEK293 cells cotransfected with GluA1/GluA2 and control vector (pcDNA3, P3), α2δ-1, α2δ-2, or α2δ-3 (n = 4 per group). ***p

    Techniques Used: Expressing, Plasmid Preparation

    α2δ-1 disrupts the heteromeric assembly of GluA1/GluA2 and increases GluA2 retention in the endoplasmic reticulum (A and B) Original blots (A) and quantification (B) show that α2δ-1 coexpression diminishes heteromeric GluA1/GluA2 receptors in the ER of HEK293 cells. n = 5 per group. *p
    Figure Legend Snippet: α2δ-1 disrupts the heteromeric assembly of GluA1/GluA2 and increases GluA2 retention in the endoplasmic reticulum (A and B) Original blots (A) and quantification (B) show that α2δ-1 coexpression diminishes heteromeric GluA1/GluA2 receptors in the ER of HEK293 cells. n = 5 per group. *p

    Techniques Used:

    α2δ-1 physically interacts with GluA1 and GluA2 in vitro and in vivo (A) CoIP shows the interaction between α2δ-1 and GluA1 and GluA2 in HEK293 cells. Cells cotransfected with GFP-tagged α2δ-1 and GluA1, GluA2, GluA1/GluA2, or FLAG-stargazin (STG). (B) CoIP shows the interaction of α2δ-1 with homomeric GluA1 or GluA2 in HEK293 cells. P3, control vector. (C) CoIP shows the interaction of α2δ-1 with heteromeric GluA1/GluA2 in HEK293 cells. (D) CoIP shows the interaction of α2δ-1 with GluA1 and GluA2 in the dorsal spinal cord of rats subjected to a sham procedure (S) or spinal nerve ligation (L). (E) CoIP shows the interaction of α2δ-1 with GluA1 and GluA2 subunits in the normal spinal cord tissue of two human donors (S1 and S2). (F) α2δ-1 interacts with GluA1 and GluA2 subunits via its C terminus. HEK293 cells were cotransfected with GluA1/GluA2 and various PC-tagged α2δ-1 constructs. δ-1ΔCT, δ-1 without the C terminus; CT, the C terminus of δ-1; VWA, von Willebrand factor type A domain. (G) CoIP shows that α2δ-1CT-Tat peptide disrupts the α2δ-1 interaction with GluA1 and GluA2 in HEK293 cells. Cell were cotransfected with GluA1/GluA2 and α2δ-1 or FLAG-α2δ-1 and were treated with 1 μM α2δ-1CT-Tat peptide (Pept) or 1 μM Tat-fused Cont peptide for 30 min. ***p
    Figure Legend Snippet: α2δ-1 physically interacts with GluA1 and GluA2 in vitro and in vivo (A) CoIP shows the interaction between α2δ-1 and GluA1 and GluA2 in HEK293 cells. Cells cotransfected with GFP-tagged α2δ-1 and GluA1, GluA2, GluA1/GluA2, or FLAG-stargazin (STG). (B) CoIP shows the interaction of α2δ-1 with homomeric GluA1 or GluA2 in HEK293 cells. P3, control vector. (C) CoIP shows the interaction of α2δ-1 with heteromeric GluA1/GluA2 in HEK293 cells. (D) CoIP shows the interaction of α2δ-1 with GluA1 and GluA2 in the dorsal spinal cord of rats subjected to a sham procedure (S) or spinal nerve ligation (L). (E) CoIP shows the interaction of α2δ-1 with GluA1 and GluA2 subunits in the normal spinal cord tissue of two human donors (S1 and S2). (F) α2δ-1 interacts with GluA1 and GluA2 subunits via its C terminus. HEK293 cells were cotransfected with GluA1/GluA2 and various PC-tagged α2δ-1 constructs. δ-1ΔCT, δ-1 without the C terminus; CT, the C terminus of δ-1; VWA, von Willebrand factor type A domain. (G) CoIP shows that α2δ-1CT-Tat peptide disrupts the α2δ-1 interaction with GluA1 and GluA2 in HEK293 cells. Cell were cotransfected with GluA1/GluA2 and α2δ-1 or FLAG-α2δ-1 and were treated with 1 μM α2δ-1CT-Tat peptide (Pept) or 1 μM Tat-fused Cont peptide for 30 min. ***p

    Techniques Used: In Vitro, In Vivo, Co-Immunoprecipitation Assay, Plasmid Preparation, Ligation, Construct

    Gabapentin and the α2δ-1CT-Tat peptide normalize synaptic expression of GluA2-containing AMPARs in the spinal cord diminished in neuropathic pain (A and B) Original blots and quantification show the effect of gabapentin and α2δ-1CT-Tat peptide on the protein levels of GluA1 and GluA2 in the spinal cord synaptosome (A) and the ER (B) of sham and SNL rats (n = 6 rats per group). Spinal cord slices were treated with vehicle (Cont), 100 μM GBP, 1 μM control peptide (P(−)), or 1 μM α2δ-1CT-Tat peptide (P(+)). **p
    Figure Legend Snippet: Gabapentin and the α2δ-1CT-Tat peptide normalize synaptic expression of GluA2-containing AMPARs in the spinal cord diminished in neuropathic pain (A and B) Original blots and quantification show the effect of gabapentin and α2δ-1CT-Tat peptide on the protein levels of GluA1 and GluA2 in the spinal cord synaptosome (A) and the ER (B) of sham and SNL rats (n = 6 rats per group). Spinal cord slices were treated with vehicle (Cont), 100 μM GBP, 1 μM control peptide (P(−)), or 1 μM α2δ-1CT-Tat peptide (P(+)). **p

    Techniques Used: Expressing

    Gabapentin and the α2δ-1CT-Tat C terminus peptide restore heteromeric GluA1/GluA2 receptors diminished by α2δ-1 coexpression (A and B) Original GCaMP images and signals show intracellular Ca 2+ changes in response to 5 mM glutamate (Glut) in HEK293 cells transfected with GluA1/GluA2 (A) or GluA1/GluA2/α2δ-1 (B). (C) Mean data show effects of treatment with vehicle (n = 54 cells), gabapentin (100 μM, n = 28 cells), α2δ-1CT-Tat peptide (1 μM, n = 26 cells), or control peptide (1 μM, n = 21 cells) on the ratio (ΔF/F0) of GCaMP signals elicited by glutamate. ***p
    Figure Legend Snippet: Gabapentin and the α2δ-1CT-Tat C terminus peptide restore heteromeric GluA1/GluA2 receptors diminished by α2δ-1 coexpression (A and B) Original GCaMP images and signals show intracellular Ca 2+ changes in response to 5 mM glutamate (Glut) in HEK293 cells transfected with GluA1/GluA2 (A) or GluA1/GluA2/α2δ-1 (B). (C) Mean data show effects of treatment with vehicle (n = 54 cells), gabapentin (100 μM, n = 28 cells), α2δ-1CT-Tat peptide (1 μM, n = 26 cells), or control peptide (1 μM, n = 21 cells) on the ratio (ΔF/F0) of GCaMP signals elicited by glutamate. ***p

    Techniques Used: Transfection

    Related Articles

    Immunoprecipitation:

    Article Title: Increased α2δ−1–NMDA receptor coupling potentiates glutamatergic input to spinal dorsal horn neurons in chemotherapy-induced neuropathic pain
    Article Snippet: .. All samples were washed 3 times with immunoprecipitation buffer and then immunoblotted with rabbit anti–α2δ−1 (#ACC-015, 1:500, Alomone Labs, Jerusalem, Israel; RRID: AB_2039785) and rabbit anti-GluN1 antibodies (#G8913, 1:1,000, Sigma-Aldrich, St. Louis, MO; RRID: AB_259978). ..

    Incubation:

    Article Title: Increased α2δ−1–NMDA receptor coupling potentiates glutamatergic input to spinal dorsal horn neurons in chemotherapy-induced neuropathic pain
    Article Snippet: .. The membrane was treated with 5% nonfat dry milk in Tris-buffered saline (TBS) at 25°C for 1 h and then incubated in TBS supplemented with 0.1% Triton X-100, 1% bovine serum albumin, and rabbit anti-α2δ−1 (#ACC-015, 1:500, Alomone Labs, Jerusalem, Israel), rabbit anti-GluN1 (#G8913, 1:1,000, Sigma-Aldrich), rabbit anti-GAPDH (#14C10, 1:5,000, Cell Signaling Technology, Danvers, MA), or mouse anti-PSD95 (#75–348, 1:1,000, NeuroMab, Davis, CA) antibodies overnight at 4°C. ..

    Article Title: Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development
    Article Snippet: .. Primary antibodies, targeting the respective HA-tagged or endogenous a2δ protein of interest as well as the loading control β-actin, were diluted (as indicated) in 5% [w/v] milk and incubated overnight at 4°C: monoclonal mouse anti-HA-tag (1:1000; OriGene Technologies, catalog #TA180128), polyclonal anti-HA-tag (1:1000; Synaptic Systems, catalog #245003), polyclonal rabbit anti human Cacna2d1 (1:200; Alomone Labs, catalog #ACC-015), polyclonal rabbit anti-Cava2δ3 (extracellular) (1:200; Santa Cruz Biotechnology, catalog #sc-99 324), polyclonal rabbit anti-CACNA2D3 (1:1000; Thermo Fisher Scientific, catalog #PA5-87 802), and monoclonal mouse anti-β-actin (1:2000; Synaptic Systems, catalog #251011). ..

    Western Blot:

    Article Title: Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy
    Article Snippet: .. Protein was analyzed by Western blot on PVDF (1620177, Cav α2δ1; Bio-Rad Laboratories) or nitrocellulose (10600003, Cav α1; GE Healthcare Life Sciences, Little Chalfont St. Giles, United Kingdom), and immunodetection was performed as previously described using antibodies specific for Cav α1 (ACC-003; Alomone Labs, Jerusalem, Israel) or Cav α2δ1 (ACC-015; Alomone Labs) subunits. ..

    Immunodetection:

    Article Title: Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy
    Article Snippet: .. Protein was analyzed by Western blot on PVDF (1620177, Cav α2δ1; Bio-Rad Laboratories) or nitrocellulose (10600003, Cav α1; GE Healthcare Life Sciences, Little Chalfont St. Giles, United Kingdom), and immunodetection was performed as previously described using antibodies specific for Cav α1 (ACC-003; Alomone Labs, Jerusalem, Israel) or Cav α2δ1 (ACC-015; Alomone Labs) subunits. ..

    FLAG-tag:

    Article Title: α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly
    Article Snippet: .. Samples were immunoblotted after being washed three times with IP buffer. α2δ-1 was detected using rabbit anti-α2δ-1 antibody (#C5105, 1:1,000; Sigma-Aldrich), GluA1 was detected using mouse anti-GluA1 antibody (#75-327, 1:1,000; NeuroMab) or rabbit anti-GluA1 antibody (#ACC-015, 1:1,000; Alomone Labs), GluA2 was detected by using mouse anti-GluA2 antibody (#75-002, 1:1,000; NeuroMab) or rabbit anti-GluA2 antibody (#AGC-005, 1:1,000; Alomone Labs), and Flag Tag was detected using mouse anti-Flag antibody (#F1804, 1:1,000; Sigma-Aldrich) or rabbit anti-Flag antibody (#F7425, 1:1,000; Sigma-Aldrich). ..

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    Alomone Labs glua2
    α2δ-1 reduces surface and synaptic expression of <t>GluA2</t> and heteromeric GluA1/GluA2 receptors (A) Original blots and quantification show the protein levels of GluA1 and GluA2 in HEK293 cells cotransfected with GluA1/GluA2 and control vector (pcDNA3, P3), α2δ-1, α2δ-2, or α2δ-3 (n = 4 per group). ***p
    Glua2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glua2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glua2 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

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    α2δ-1 reduces surface and synaptic expression of GluA2 and heteromeric GluA1/GluA2 receptors (A) Original blots and quantification show the protein levels of GluA1 and GluA2 in HEK293 cells cotransfected with GluA1/GluA2 and control vector (pcDNA3, P3), α2δ-1, α2δ-2, or α2δ-3 (n = 4 per group). ***p

    Journal: Cell reports

    Article Title: α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly

    doi: 10.1016/j.celrep.2021.109396

    Figure Lengend Snippet: α2δ-1 reduces surface and synaptic expression of GluA2 and heteromeric GluA1/GluA2 receptors (A) Original blots and quantification show the protein levels of GluA1 and GluA2 in HEK293 cells cotransfected with GluA1/GluA2 and control vector (pcDNA3, P3), α2δ-1, α2δ-2, or α2δ-3 (n = 4 per group). ***p

    Article Snippet: Samples were immunoblotted after being washed three times with IP buffer. α2δ-1 was detected using rabbit anti-α2δ-1 antibody (#C5105, 1:1,000; Sigma-Aldrich), GluA1 was detected using mouse anti-GluA1 antibody (#75-327, 1:1,000; NeuroMab) or rabbit anti-GluA1 antibody (#ACC-015, 1:1,000; Alomone Labs), GluA2 was detected by using mouse anti-GluA2 antibody (#75-002, 1:1,000; NeuroMab) or rabbit anti-GluA2 antibody (#AGC-005, 1:1,000; Alomone Labs), and Flag Tag was detected using mouse anti-Flag antibody (#F1804, 1:1,000; Sigma-Aldrich) or rabbit anti-Flag antibody (#F7425, 1:1,000; Sigma-Aldrich).

    Techniques: Expressing, Plasmid Preparation

    α2δ-1 disrupts the heteromeric assembly of GluA1/GluA2 and increases GluA2 retention in the endoplasmic reticulum (A and B) Original blots (A) and quantification (B) show that α2δ-1 coexpression diminishes heteromeric GluA1/GluA2 receptors in the ER of HEK293 cells. n = 5 per group. *p

    Journal: Cell reports

    Article Title: α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly

    doi: 10.1016/j.celrep.2021.109396

    Figure Lengend Snippet: α2δ-1 disrupts the heteromeric assembly of GluA1/GluA2 and increases GluA2 retention in the endoplasmic reticulum (A and B) Original blots (A) and quantification (B) show that α2δ-1 coexpression diminishes heteromeric GluA1/GluA2 receptors in the ER of HEK293 cells. n = 5 per group. *p

    Article Snippet: Samples were immunoblotted after being washed three times with IP buffer. α2δ-1 was detected using rabbit anti-α2δ-1 antibody (#C5105, 1:1,000; Sigma-Aldrich), GluA1 was detected using mouse anti-GluA1 antibody (#75-327, 1:1,000; NeuroMab) or rabbit anti-GluA1 antibody (#ACC-015, 1:1,000; Alomone Labs), GluA2 was detected by using mouse anti-GluA2 antibody (#75-002, 1:1,000; NeuroMab) or rabbit anti-GluA2 antibody (#AGC-005, 1:1,000; Alomone Labs), and Flag Tag was detected using mouse anti-Flag antibody (#F1804, 1:1,000; Sigma-Aldrich) or rabbit anti-Flag antibody (#F7425, 1:1,000; Sigma-Aldrich).

    Techniques:

    α2δ-1 physically interacts with GluA1 and GluA2 in vitro and in vivo (A) CoIP shows the interaction between α2δ-1 and GluA1 and GluA2 in HEK293 cells. Cells cotransfected with GFP-tagged α2δ-1 and GluA1, GluA2, GluA1/GluA2, or FLAG-stargazin (STG). (B) CoIP shows the interaction of α2δ-1 with homomeric GluA1 or GluA2 in HEK293 cells. P3, control vector. (C) CoIP shows the interaction of α2δ-1 with heteromeric GluA1/GluA2 in HEK293 cells. (D) CoIP shows the interaction of α2δ-1 with GluA1 and GluA2 in the dorsal spinal cord of rats subjected to a sham procedure (S) or spinal nerve ligation (L). (E) CoIP shows the interaction of α2δ-1 with GluA1 and GluA2 subunits in the normal spinal cord tissue of two human donors (S1 and S2). (F) α2δ-1 interacts with GluA1 and GluA2 subunits via its C terminus. HEK293 cells were cotransfected with GluA1/GluA2 and various PC-tagged α2δ-1 constructs. δ-1ΔCT, δ-1 without the C terminus; CT, the C terminus of δ-1; VWA, von Willebrand factor type A domain. (G) CoIP shows that α2δ-1CT-Tat peptide disrupts the α2δ-1 interaction with GluA1 and GluA2 in HEK293 cells. Cell were cotransfected with GluA1/GluA2 and α2δ-1 or FLAG-α2δ-1 and were treated with 1 μM α2δ-1CT-Tat peptide (Pept) or 1 μM Tat-fused Cont peptide for 30 min. ***p

    Journal: Cell reports

    Article Title: α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly

    doi: 10.1016/j.celrep.2021.109396

    Figure Lengend Snippet: α2δ-1 physically interacts with GluA1 and GluA2 in vitro and in vivo (A) CoIP shows the interaction between α2δ-1 and GluA1 and GluA2 in HEK293 cells. Cells cotransfected with GFP-tagged α2δ-1 and GluA1, GluA2, GluA1/GluA2, or FLAG-stargazin (STG). (B) CoIP shows the interaction of α2δ-1 with homomeric GluA1 or GluA2 in HEK293 cells. P3, control vector. (C) CoIP shows the interaction of α2δ-1 with heteromeric GluA1/GluA2 in HEK293 cells. (D) CoIP shows the interaction of α2δ-1 with GluA1 and GluA2 in the dorsal spinal cord of rats subjected to a sham procedure (S) or spinal nerve ligation (L). (E) CoIP shows the interaction of α2δ-1 with GluA1 and GluA2 subunits in the normal spinal cord tissue of two human donors (S1 and S2). (F) α2δ-1 interacts with GluA1 and GluA2 subunits via its C terminus. HEK293 cells were cotransfected with GluA1/GluA2 and various PC-tagged α2δ-1 constructs. δ-1ΔCT, δ-1 without the C terminus; CT, the C terminus of δ-1; VWA, von Willebrand factor type A domain. (G) CoIP shows that α2δ-1CT-Tat peptide disrupts the α2δ-1 interaction with GluA1 and GluA2 in HEK293 cells. Cell were cotransfected with GluA1/GluA2 and α2δ-1 or FLAG-α2δ-1 and were treated with 1 μM α2δ-1CT-Tat peptide (Pept) or 1 μM Tat-fused Cont peptide for 30 min. ***p

    Article Snippet: Samples were immunoblotted after being washed three times with IP buffer. α2δ-1 was detected using rabbit anti-α2δ-1 antibody (#C5105, 1:1,000; Sigma-Aldrich), GluA1 was detected using mouse anti-GluA1 antibody (#75-327, 1:1,000; NeuroMab) or rabbit anti-GluA1 antibody (#ACC-015, 1:1,000; Alomone Labs), GluA2 was detected by using mouse anti-GluA2 antibody (#75-002, 1:1,000; NeuroMab) or rabbit anti-GluA2 antibody (#AGC-005, 1:1,000; Alomone Labs), and Flag Tag was detected using mouse anti-Flag antibody (#F1804, 1:1,000; Sigma-Aldrich) or rabbit anti-Flag antibody (#F7425, 1:1,000; Sigma-Aldrich).

    Techniques: In Vitro, In Vivo, Co-Immunoprecipitation Assay, Plasmid Preparation, Ligation, Construct

    Gabapentin and the α2δ-1CT-Tat peptide normalize synaptic expression of GluA2-containing AMPARs in the spinal cord diminished in neuropathic pain (A and B) Original blots and quantification show the effect of gabapentin and α2δ-1CT-Tat peptide on the protein levels of GluA1 and GluA2 in the spinal cord synaptosome (A) and the ER (B) of sham and SNL rats (n = 6 rats per group). Spinal cord slices were treated with vehicle (Cont), 100 μM GBP, 1 μM control peptide (P(−)), or 1 μM α2δ-1CT-Tat peptide (P(+)). **p

    Journal: Cell reports

    Article Title: α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly

    doi: 10.1016/j.celrep.2021.109396

    Figure Lengend Snippet: Gabapentin and the α2δ-1CT-Tat peptide normalize synaptic expression of GluA2-containing AMPARs in the spinal cord diminished in neuropathic pain (A and B) Original blots and quantification show the effect of gabapentin and α2δ-1CT-Tat peptide on the protein levels of GluA1 and GluA2 in the spinal cord synaptosome (A) and the ER (B) of sham and SNL rats (n = 6 rats per group). Spinal cord slices were treated with vehicle (Cont), 100 μM GBP, 1 μM control peptide (P(−)), or 1 μM α2δ-1CT-Tat peptide (P(+)). **p

    Article Snippet: Samples were immunoblotted after being washed three times with IP buffer. α2δ-1 was detected using rabbit anti-α2δ-1 antibody (#C5105, 1:1,000; Sigma-Aldrich), GluA1 was detected using mouse anti-GluA1 antibody (#75-327, 1:1,000; NeuroMab) or rabbit anti-GluA1 antibody (#ACC-015, 1:1,000; Alomone Labs), GluA2 was detected by using mouse anti-GluA2 antibody (#75-002, 1:1,000; NeuroMab) or rabbit anti-GluA2 antibody (#AGC-005, 1:1,000; Alomone Labs), and Flag Tag was detected using mouse anti-Flag antibody (#F1804, 1:1,000; Sigma-Aldrich) or rabbit anti-Flag antibody (#F7425, 1:1,000; Sigma-Aldrich).

    Techniques: Expressing

    Gabapentin and the α2δ-1CT-Tat C terminus peptide restore heteromeric GluA1/GluA2 receptors diminished by α2δ-1 coexpression (A and B) Original GCaMP images and signals show intracellular Ca 2+ changes in response to 5 mM glutamate (Glut) in HEK293 cells transfected with GluA1/GluA2 (A) or GluA1/GluA2/α2δ-1 (B). (C) Mean data show effects of treatment with vehicle (n = 54 cells), gabapentin (100 μM, n = 28 cells), α2δ-1CT-Tat peptide (1 μM, n = 26 cells), or control peptide (1 μM, n = 21 cells) on the ratio (ΔF/F0) of GCaMP signals elicited by glutamate. ***p

    Journal: Cell reports

    Article Title: α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly

    doi: 10.1016/j.celrep.2021.109396

    Figure Lengend Snippet: Gabapentin and the α2δ-1CT-Tat C terminus peptide restore heteromeric GluA1/GluA2 receptors diminished by α2δ-1 coexpression (A and B) Original GCaMP images and signals show intracellular Ca 2+ changes in response to 5 mM glutamate (Glut) in HEK293 cells transfected with GluA1/GluA2 (A) or GluA1/GluA2/α2δ-1 (B). (C) Mean data show effects of treatment with vehicle (n = 54 cells), gabapentin (100 μM, n = 28 cells), α2δ-1CT-Tat peptide (1 μM, n = 26 cells), or control peptide (1 μM, n = 21 cells) on the ratio (ΔF/F0) of GCaMP signals elicited by glutamate. ***p

    Article Snippet: Samples were immunoblotted after being washed three times with IP buffer. α2δ-1 was detected using rabbit anti-α2δ-1 antibody (#C5105, 1:1,000; Sigma-Aldrich), GluA1 was detected using mouse anti-GluA1 antibody (#75-327, 1:1,000; NeuroMab) or rabbit anti-GluA1 antibody (#ACC-015, 1:1,000; Alomone Labs), GluA2 was detected by using mouse anti-GluA2 antibody (#75-002, 1:1,000; NeuroMab) or rabbit anti-GluA2 antibody (#AGC-005, 1:1,000; Alomone Labs), and Flag Tag was detected using mouse anti-Flag antibody (#F1804, 1:1,000; Sigma-Aldrich) or rabbit anti-Flag antibody (#F7425, 1:1,000; Sigma-Aldrich).

    Techniques: Transfection