ncx2  (Alomone Labs)


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    Alomone Labs ncx2
    Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of <t>NCX2</t> were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P
    Ncx2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncx2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncx2 - by Bioz Stars, 2022-10
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    1) Product Images from "Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis"

    Article Title: Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis

    Journal: Experimental & Molecular Medicine

    doi: 10.1038/s12276-018-0088-z

    Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of NCX2 were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P
    Figure Legend Snippet: Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of NCX2 were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P

    Techniques Used: Expressing, Immunocytochemistry, Staining, Isolation, Mass Spectrometry

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    Alomone Labs ncx2
    Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of <t>NCX2</t> were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P
    Ncx2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncx2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncx2 - by Bioz Stars, 2022-10
    94/100 stars
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    Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of NCX2 were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P

    Journal: Experimental & Molecular Medicine

    Article Title: Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis

    doi: 10.1038/s12276-018-0088-z

    Figure Lengend Snippet: Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of NCX2 were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P

    Article Snippet: After being blocked with 5% skim milk dissolved in Tris-buffered saline at room temperature for 2 h, the membranes were incubated overnight at 4 °C with various primary antibodies targeting the following proteins: IL-6 (Abcam, Cambridge, UK, ab9324, 1:800), TNF-α (Abcam, ab199013, 1:500), Piezo1 (Alomone labs, Jerusalem, Israel, APC-087, 1:500), NCX1 (Abcam, ab2869, 1:800), NCX2 (Alomone labs, ANX-012, 1:500), NCX3 (Alomone labs, ANX-013, 1:500), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Beyotime, AG019, 1:1000), and α-tubulin (Beyotime, AT819, 1:1000).

    Techniques: Expressing, Immunocytochemistry, Staining, Isolation, Mass Spectrometry