anti ncx1 slc8a1 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti ncx1 slc8a1 antibody
    <t>NCX1</t> coordinates with TRPC1 to promote proliferation and migration of human GC cells. Summary data showing the inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (1 mM)-enhanced proliferation ( A – C ) and migration ( G – I ) of GC cells. D–F , J–L Summary data showing the inhibitory effect of either shNCX1, SKF96365 (SKF, 1 μM) or shNCX1 plus SKF on CaCl 2 (1 mM)-enhanced proliferation and migration of GC cells. Scale bar = 200 μm for each image. ( * P
    Anti Ncx1 Slc8a1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ncx1 slc8a1 antibody/product/Alomone Labs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti ncx1 slc8a1 antibody - by Bioz Stars, 2022-12
    94/100 stars

    Images

    1) Product Images from "NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway"

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway

    Journal: Oncogene

    doi: 10.1038/s41388-022-02412-9

    NCX1 coordinates with TRPC1 to promote proliferation and migration of human GC cells. Summary data showing the inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (1 mM)-enhanced proliferation ( A – C ) and migration ( G – I ) of GC cells. D–F , J–L Summary data showing the inhibitory effect of either shNCX1, SKF96365 (SKF, 1 μM) or shNCX1 plus SKF on CaCl 2 (1 mM)-enhanced proliferation and migration of GC cells. Scale bar = 200 μm for each image. ( * P
    Figure Legend Snippet: NCX1 coordinates with TRPC1 to promote proliferation and migration of human GC cells. Summary data showing the inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (1 mM)-enhanced proliferation ( A – C ) and migration ( G – I ) of GC cells. D–F , J–L Summary data showing the inhibitory effect of either shNCX1, SKF96365 (SKF, 1 μM) or shNCX1 plus SKF on CaCl 2 (1 mM)-enhanced proliferation and migration of GC cells. Scale bar = 200 μm for each image. ( * P

    Techniques Used: Migration

    TRPC1/NCX1 coupling induces AKT phosphorylation and promotes GC growth and metastasis. A , D Inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. B , E Inhibitory effects of SKF, shNCX1 or shNCX1 plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. C , F Inhibitory effects of either KB-R, shTRPC1 or shTRPC1 plus KB-R on CaCl 2 -induced AKT phosphorylation in GC cells. CaCl 2 promoted growth of xenografted gastric tumors ( G ), which was attenuated by either KB-R7943 ( H ) or shNCX1 ( I ). J Inhibitory effects of shNCX1 on CaCl 2 -induced gastric tumor metastasis. K Immunohistochemical analysis and histological examination on expression of NCX1 and Ki67 proteins with or without NCX1 knockdown in GC tissues. Scale bar = 100 μm for each image. L , M Summary data comparing expression of NCX1 and Ki67 proteins analyzed by immunohistochemistry between with or without NCX1 knockdown in GC tissues. ( * P
    Figure Legend Snippet: TRPC1/NCX1 coupling induces AKT phosphorylation and promotes GC growth and metastasis. A , D Inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. B , E Inhibitory effects of SKF, shNCX1 or shNCX1 plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. C , F Inhibitory effects of either KB-R, shTRPC1 or shTRPC1 plus KB-R on CaCl 2 -induced AKT phosphorylation in GC cells. CaCl 2 promoted growth of xenografted gastric tumors ( G ), which was attenuated by either KB-R7943 ( H ) or shNCX1 ( I ). J Inhibitory effects of shNCX1 on CaCl 2 -induced gastric tumor metastasis. K Immunohistochemical analysis and histological examination on expression of NCX1 and Ki67 proteins with or without NCX1 knockdown in GC tissues. Scale bar = 100 μm for each image. L , M Summary data comparing expression of NCX1 and Ki67 proteins analyzed by immunohistochemistry between with or without NCX1 knockdown in GC tissues. ( * P

    Techniques Used: Immunohistochemistry, Expressing

    Hp virulence factor and acid stimulate NCX1/TRPC1 coupling in human GC cells. Summary tracings of [Ca 2+ ] cyt time courses in response to extracellular 0 Na + ( A ), NH 4 Cl (5 mM) ( C ) and pH 4.5 ( E ) in the presence of extracellular 2 Ca 2+ or 0 Ca 2+ (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( A , n = 20 cells; C , n = 11 cells; E , n = 11 cells, **** P
    Figure Legend Snippet: Hp virulence factor and acid stimulate NCX1/TRPC1 coupling in human GC cells. Summary tracings of [Ca 2+ ] cyt time courses in response to extracellular 0 Na + ( A ), NH 4 Cl (5 mM) ( C ) and pH 4.5 ( E ) in the presence of extracellular 2 Ca 2+ or 0 Ca 2+ (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( A , n = 20 cells; C , n = 11 cells; E , n = 11 cells, **** P

    Techniques Used:

    NCX1 activation induces phosphorylation of AKT and β-catenin in human GC cells. A , B , E , F Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS) on CaCl 2 (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. C , D , G , H Inhibitory effect of KB-R7943 on NH 4 Cl (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. I , J , M , N Inhibitory effect of shNCX1 on CaCl 2 -induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. K , L , O , P Inhibitory effect of shNCX1 on NH 4 Cl-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. ( * P
    Figure Legend Snippet: NCX1 activation induces phosphorylation of AKT and β-catenin in human GC cells. A , B , E , F Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS) on CaCl 2 (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. C , D , G , H Inhibitory effect of KB-R7943 on NH 4 Cl (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. I , J , M , N Inhibitory effect of shNCX1 on CaCl 2 -induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. K , L , O , P Inhibitory effect of shNCX1 on NH 4 Cl-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. ( * P

    Techniques Used: Activation Assay

    The enhanced expression of NCX1 and TRPC1 in human primary gastric cancer tissues and cells. Western blot analysis applied to compare the expression levels of NCX1 proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P
    Figure Legend Snippet: The enhanced expression of NCX1 and TRPC1 in human primary gastric cancer tissues and cells. Western blot analysis applied to compare the expression levels of NCX1 proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P

    Techniques Used: Expressing, Western Blot, Staining, Negative Control

    NCX1 activation promotes proliferation and invasion of human GC cells. Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in MKN45 ( A ), AGS ( D ), and SGC7901 ( G ) cells. The inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) on CaCl 2 (1 mM)-induced proliferation ( B , E , H ) and invasion ( C , F , I ) of GC cells, Scale bar=200 μm for each image. Dose-dependently enhanced proliferation of NH 4 Cl (0.1-2 mM) in MKN45 ( J ), AGS ( L ), and SGC7901 ( N ) cells, and the inhibitory effect of KB-R7943 on NH 4 Cl (1 mM)-induced proliferation of MKN45 ( K ), AGS ( M ), and SGC7901 ( O ) cells. P , Q Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in CHO-NCX1 with NCX1 overexpression, and the inhibitory effect of KB-R7943 (0.2 μM) on CaCl 2 (1 mM)-induced proliferation of CHO-NCX1 cells. R–U No effects of CaCl 2 (0.1–2 mM) and NH 4 Cl (0.1–2 mM) on proliferation of CHO-K1 without NCX1 overexpression and GES1 cells. ( * P
    Figure Legend Snippet: NCX1 activation promotes proliferation and invasion of human GC cells. Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in MKN45 ( A ), AGS ( D ), and SGC7901 ( G ) cells. The inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) on CaCl 2 (1 mM)-induced proliferation ( B , E , H ) and invasion ( C , F , I ) of GC cells, Scale bar=200 μm for each image. Dose-dependently enhanced proliferation of NH 4 Cl (0.1-2 mM) in MKN45 ( J ), AGS ( L ), and SGC7901 ( N ) cells, and the inhibitory effect of KB-R7943 on NH 4 Cl (1 mM)-induced proliferation of MKN45 ( K ), AGS ( M ), and SGC7901 ( O ) cells. P , Q Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in CHO-NCX1 with NCX1 overexpression, and the inhibitory effect of KB-R7943 (0.2 μM) on CaCl 2 (1 mM)-induced proliferation of CHO-NCX1 cells. R–U No effects of CaCl 2 (0.1–2 mM) and NH 4 Cl (0.1–2 mM) on proliferation of CHO-K1 without NCX1 overexpression and GES1 cells. ( * P

    Techniques Used: Activation Assay, Over Expression

    CaCl 2 , Hp and virulence factors enhance NCX1 expression in human GC cells. A , G , M Representative time courses of CaCl 2 (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) ( B , H , N ) or shNCX1 ( C , I , O ) on CaCl 2 (2 mM)-enhanced NCX1 expression in GC cells. D , J , P Representative time courses of NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 ( E , K , Q ) or shNCX1 ( F , L , R ) on NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. S , T , U Representative time courses LPS (10 ng/ml)-enhanced NCX1 protein expression in GC cells. V , W Representative time courses H. pylori -enhanced NCX1 protein in GC cells. ( * P
    Figure Legend Snippet: CaCl 2 , Hp and virulence factors enhance NCX1 expression in human GC cells. A , G , M Representative time courses of CaCl 2 (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) ( B , H , N ) or shNCX1 ( C , I , O ) on CaCl 2 (2 mM)-enhanced NCX1 expression in GC cells. D , J , P Representative time courses of NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 ( E , K , Q ) or shNCX1 ( F , L , R ) on NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. S , T , U Representative time courses LPS (10 ng/ml)-enhanced NCX1 protein expression in GC cells. V , W Representative time courses H. pylori -enhanced NCX1 protein in GC cells. ( * P

    Techniques Used: Expressing

    CaCl 2 and NH 4 Cl promote proliferation and invasion of human GC cells through NCX1 activation. Representative images of NCX1 protein expression in GC cells with NCX1 knockdown and summary data of NCX1 protein levels in MKN45 ( A ), AGS ( B ), and SGC7901 ( C ) cells ( * P
    Figure Legend Snippet: CaCl 2 and NH 4 Cl promote proliferation and invasion of human GC cells through NCX1 activation. Representative images of NCX1 protein expression in GC cells with NCX1 knockdown and summary data of NCX1 protein levels in MKN45 ( A ), AGS ( B ), and SGC7901 ( C ) cells ( * P

    Techniques Used: Activation Assay, Expressing

    2) Product Images from "NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway"

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway

    Journal: Oncogene

    doi: 10.1038/s41388-022-02412-9

    NCX1 coordinates with TRPC1 to promote proliferation and migration of human GC cells. Summary data showing the inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (1 mM)-enhanced proliferation ( A – C ) and migration ( G – I ) of GC cells. D–F , J–L Summary data showing the inhibitory effect of either shNCX1, SKF96365 (SKF, 1 μM) or shNCX1 plus SKF on CaCl 2 (1 mM)-enhanced proliferation and migration of GC cells. Scale bar = 200 μm for each image. ( * P
    Figure Legend Snippet: NCX1 coordinates with TRPC1 to promote proliferation and migration of human GC cells. Summary data showing the inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (1 mM)-enhanced proliferation ( A – C ) and migration ( G – I ) of GC cells. D–F , J–L Summary data showing the inhibitory effect of either shNCX1, SKF96365 (SKF, 1 μM) or shNCX1 plus SKF on CaCl 2 (1 mM)-enhanced proliferation and migration of GC cells. Scale bar = 200 μm for each image. ( * P

    Techniques Used: Migration

    TRPC1/NCX1 coupling induces AKT phosphorylation and promotes GC growth and metastasis. A , D Inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. B , E Inhibitory effects of SKF, shNCX1 or shNCX1 plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. C , F Inhibitory effects of either KB-R, shTRPC1 or shTRPC1 plus KB-R on CaCl 2 -induced AKT phosphorylation in GC cells. CaCl 2 promoted growth of xenografted gastric tumors ( G ), which was attenuated by either KB-R7943 ( H ) or shNCX1 ( I ). J Inhibitory effects of shNCX1 on CaCl 2 -induced gastric tumor metastasis. K Immunohistochemical analysis and histological examination on expression of NCX1 and Ki67 proteins with or without NCX1 knockdown in GC tissues. Scale bar = 100 μm for each image. L , M Summary data comparing expression of NCX1 and Ki67 proteins analyzed by immunohistochemistry between with or without NCX1 knockdown in GC tissues. ( * P
    Figure Legend Snippet: TRPC1/NCX1 coupling induces AKT phosphorylation and promotes GC growth and metastasis. A , D Inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. B , E Inhibitory effects of SKF, shNCX1 or shNCX1 plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. C , F Inhibitory effects of either KB-R, shTRPC1 or shTRPC1 plus KB-R on CaCl 2 -induced AKT phosphorylation in GC cells. CaCl 2 promoted growth of xenografted gastric tumors ( G ), which was attenuated by either KB-R7943 ( H ) or shNCX1 ( I ). J Inhibitory effects of shNCX1 on CaCl 2 -induced gastric tumor metastasis. K Immunohistochemical analysis and histological examination on expression of NCX1 and Ki67 proteins with or without NCX1 knockdown in GC tissues. Scale bar = 100 μm for each image. L , M Summary data comparing expression of NCX1 and Ki67 proteins analyzed by immunohistochemistry between with or without NCX1 knockdown in GC tissues. ( * P

    Techniques Used: Immunohistochemistry, Expressing

    Hp virulence factor and acid stimulate NCX1/TRPC1 coupling in human GC cells. Summary tracings of [Ca 2+ ] cyt time courses in response to extracellular 0 Na + ( A ), NH 4 Cl (5 mM) ( C ) and pH 4.5 ( E ) in the presence of extracellular 2 Ca 2+ or 0 Ca 2+ (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( A , n = 20 cells; C , n = 11 cells; E , n = 11 cells, **** P
    Figure Legend Snippet: Hp virulence factor and acid stimulate NCX1/TRPC1 coupling in human GC cells. Summary tracings of [Ca 2+ ] cyt time courses in response to extracellular 0 Na + ( A ), NH 4 Cl (5 mM) ( C ) and pH 4.5 ( E ) in the presence of extracellular 2 Ca 2+ or 0 Ca 2+ (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( A , n = 20 cells; C , n = 11 cells; E , n = 11 cells, **** P

    Techniques Used:

    NCX1 activation induces phosphorylation of AKT and β-catenin in human GC cells. A , B , E , F Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS) on CaCl 2 (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. C , D , G , H Inhibitory effect of KB-R7943 on NH 4 Cl (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. I , J , M , N Inhibitory effect of shNCX1 on CaCl 2 -induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. K , L , O , P Inhibitory effect of shNCX1 on NH 4 Cl-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. ( * P
    Figure Legend Snippet: NCX1 activation induces phosphorylation of AKT and β-catenin in human GC cells. A , B , E , F Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS) on CaCl 2 (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. C , D , G , H Inhibitory effect of KB-R7943 on NH 4 Cl (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. I , J , M , N Inhibitory effect of shNCX1 on CaCl 2 -induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. K , L , O , P Inhibitory effect of shNCX1 on NH 4 Cl-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. ( * P

    Techniques Used: Activation Assay

    The enhanced expression of NCX1 and TRPC1 in human primary gastric cancer tissues and cells. Western blot analysis applied to compare the expression levels of NCX1 proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P
    Figure Legend Snippet: The enhanced expression of NCX1 and TRPC1 in human primary gastric cancer tissues and cells. Western blot analysis applied to compare the expression levels of NCX1 proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P

    Techniques Used: Expressing, Western Blot, Staining, Negative Control

    NCX1 activation promotes proliferation and invasion of human GC cells. Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in MKN45 ( A ), AGS ( D ), and SGC7901 ( G ) cells. The inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) on CaCl 2 (1 mM)-induced proliferation ( B , E , H ) and invasion ( C , F , I ) of GC cells, Scale bar=200 μm for each image. Dose-dependently enhanced proliferation of NH 4 Cl (0.1-2 mM) in MKN45 ( J ), AGS ( L ), and SGC7901 ( N ) cells, and the inhibitory effect of KB-R7943 on NH 4 Cl (1 mM)-induced proliferation of MKN45 ( K ), AGS ( M ), and SGC7901 ( O ) cells. P , Q Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in CHO-NCX1 with NCX1 overexpression, and the inhibitory effect of KB-R7943 (0.2 μM) on CaCl 2 (1 mM)-induced proliferation of CHO-NCX1 cells. R–U No effects of CaCl 2 (0.1–2 mM) and NH 4 Cl (0.1–2 mM) on proliferation of CHO-K1 without NCX1 overexpression and GES1 cells. ( * P
    Figure Legend Snippet: NCX1 activation promotes proliferation and invasion of human GC cells. Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in MKN45 ( A ), AGS ( D ), and SGC7901 ( G ) cells. The inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) on CaCl 2 (1 mM)-induced proliferation ( B , E , H ) and invasion ( C , F , I ) of GC cells, Scale bar=200 μm for each image. Dose-dependently enhanced proliferation of NH 4 Cl (0.1-2 mM) in MKN45 ( J ), AGS ( L ), and SGC7901 ( N ) cells, and the inhibitory effect of KB-R7943 on NH 4 Cl (1 mM)-induced proliferation of MKN45 ( K ), AGS ( M ), and SGC7901 ( O ) cells. P , Q Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in CHO-NCX1 with NCX1 overexpression, and the inhibitory effect of KB-R7943 (0.2 μM) on CaCl 2 (1 mM)-induced proliferation of CHO-NCX1 cells. R–U No effects of CaCl 2 (0.1–2 mM) and NH 4 Cl (0.1–2 mM) on proliferation of CHO-K1 without NCX1 overexpression and GES1 cells. ( * P

    Techniques Used: Activation Assay, Over Expression

    CaCl 2 , Hp and virulence factors enhance NCX1 expression in human GC cells. A , G , M Representative time courses of CaCl 2 (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) ( B , H , N ) or shNCX1 ( C , I , O ) on CaCl 2 (2 mM)-enhanced NCX1 expression in GC cells. D , J , P Representative time courses of NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 ( E , K , Q ) or shNCX1 ( F , L , R ) on NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. S , T , U Representative time courses LPS (10 ng/ml)-enhanced NCX1 protein expression in GC cells. V , W Representative time courses H. pylori -enhanced NCX1 protein in GC cells. ( * P
    Figure Legend Snippet: CaCl 2 , Hp and virulence factors enhance NCX1 expression in human GC cells. A , G , M Representative time courses of CaCl 2 (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) ( B , H , N ) or shNCX1 ( C , I , O ) on CaCl 2 (2 mM)-enhanced NCX1 expression in GC cells. D , J , P Representative time courses of NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 ( E , K , Q ) or shNCX1 ( F , L , R ) on NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. S , T , U Representative time courses LPS (10 ng/ml)-enhanced NCX1 protein expression in GC cells. V , W Representative time courses H. pylori -enhanced NCX1 protein in GC cells. ( * P

    Techniques Used: Expressing

    CaCl 2 and NH 4 Cl promote proliferation and invasion of human GC cells through NCX1 activation. Representative images of NCX1 protein expression in GC cells with NCX1 knockdown and summary data of NCX1 protein levels in MKN45 ( A ), AGS ( B ), and SGC7901 ( C ) cells ( * P
    Figure Legend Snippet: CaCl 2 and NH 4 Cl promote proliferation and invasion of human GC cells through NCX1 activation. Representative images of NCX1 protein expression in GC cells with NCX1 knockdown and summary data of NCX1 protein levels in MKN45 ( A ), AGS ( B ), and SGC7901 ( C ) cells ( * P

    Techniques Used: Activation Assay, Expressing

    3) Product Images from "Migraine‐Associated Mutation in the Na,K‐ATPase Leads to Disturbances in Cardiac Metabolism and Reduced Cardiac Function"

    Article Title: Migraine‐Associated Mutation in the Na,K‐ATPase Leads to Disturbances in Cardiac Metabolism and Reduced Cardiac Function

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    doi: 10.1161/JAHA.121.021814

    Changed expression of the Na,K‐ATPase α isoforms in the heart from α 2 +/G301R mice but similar expression of the Na,Ca‐exchanger‐1. The hearts from 3‐ and 8‐month‐old α 2 +/G301R mice showed reduced expression of the Na,K‐ATPase α 2 isoform ( A , n=7; D , n=6), increased expression of Na,K‐ATPase α 1 isoform ( B , n=7; E , n=5 to 6), and no change in the expression of Na,Ca‐exchager‐1 (NCX1; C , n=7; F , n=12) in comparison with wild type (WT). The expression profile was the same in hearts of 3‐month‐old ( A – C ) and 8‐month‐old ( D – F ) mice. Upper part of each panel shows representative Western blot bands for the averaged data shown below. The representative bands are shown from the same membrane, as well as the molecular marker shown to the right. The images cropped to include molecular weights from 250 to 50 kDa, as indicated. Total protein load was detected with the stain‐free gel, and the corresponding representative bands were cropped to the same molecular weight range. Protein expression was compared using unpaired t ‐test. * P
    Figure Legend Snippet: Changed expression of the Na,K‐ATPase α isoforms in the heart from α 2 +/G301R mice but similar expression of the Na,Ca‐exchanger‐1. The hearts from 3‐ and 8‐month‐old α 2 +/G301R mice showed reduced expression of the Na,K‐ATPase α 2 isoform ( A , n=7; D , n=6), increased expression of Na,K‐ATPase α 1 isoform ( B , n=7; E , n=5 to 6), and no change in the expression of Na,Ca‐exchager‐1 (NCX1; C , n=7; F , n=12) in comparison with wild type (WT). The expression profile was the same in hearts of 3‐month‐old ( A – C ) and 8‐month‐old ( D – F ) mice. Upper part of each panel shows representative Western blot bands for the averaged data shown below. The representative bands are shown from the same membrane, as well as the molecular marker shown to the right. The images cropped to include molecular weights from 250 to 50 kDa, as indicated. Total protein load was detected with the stain‐free gel, and the corresponding representative bands were cropped to the same molecular weight range. Protein expression was compared using unpaired t ‐test. * P

    Techniques Used: Expressing, Mouse Assay, Western Blot, Marker, Staining, Molecular Weight

    4) Product Images from "The Na+/Ca2+ Exchanger 3 Is Functionally Coupled With the NaV1.6 Voltage-Gated Channel and Promotes an Endoplasmic Reticulum Ca2+ Refilling in a Transgenic Model of Alzheimer’s Disease"

    Article Title: The Na+/Ca2+ Exchanger 3 Is Functionally Coupled With the NaV1.6 Voltage-Gated Channel and Promotes an Endoplasmic Reticulum Ca2+ Refilling in a Transgenic Model of Alzheimer’s Disease

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.775271

    Effect of NCX3 silencing or inhibition by KB-R7943 in WT and Tg2576 primary hippocampal neurons. (A) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded in WT and WT plus siNCX3 (black traces), Tg2576 and Tg2576 plus siNCX3 (grey traces) primary hippocampal neurons at 12 DIV. (B) Quantification of I NCX in the reverse mode of operation represented in A, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. (C) Representative Western blotting experiments and relative quantifications showing the effect of NCX3 silencing (siNCX3) on NCX3, NCX1, and NCX2 protein expression in primary hippocampal neurons (D) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded from WT and WT plus 0.5 μM kB-R7943 (black traces), Tg2576 and Tg2576 plus 0.5 μM kB-R7943 (grey traces) primary hippocampal neurons at 12 DIV. (E) Quantification of I NCX in the reverse mode of operation represented in D, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. The number of cells used for each experimental condition is noted on the bars. (F, G) Representative Western blot of NCX3 protein expression and densitometric quantification of NCX3 truncated band in WT and Tg2576 primary hippocampal neurons at 12 DIV, represented as percentage of WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. Statistical comparisons between groups were performed by one-way ANOVA followed by Newman-Keuls’ test. (* p
    Figure Legend Snippet: Effect of NCX3 silencing or inhibition by KB-R7943 in WT and Tg2576 primary hippocampal neurons. (A) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded in WT and WT plus siNCX3 (black traces), Tg2576 and Tg2576 plus siNCX3 (grey traces) primary hippocampal neurons at 12 DIV. (B) Quantification of I NCX in the reverse mode of operation represented in A, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. (C) Representative Western blotting experiments and relative quantifications showing the effect of NCX3 silencing (siNCX3) on NCX3, NCX1, and NCX2 protein expression in primary hippocampal neurons (D) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded from WT and WT plus 0.5 μM kB-R7943 (black traces), Tg2576 and Tg2576 plus 0.5 μM kB-R7943 (grey traces) primary hippocampal neurons at 12 DIV. (E) Quantification of I NCX in the reverse mode of operation represented in D, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. The number of cells used for each experimental condition is noted on the bars. (F, G) Representative Western blot of NCX3 protein expression and densitometric quantification of NCX3 truncated band in WT and Tg2576 primary hippocampal neurons at 12 DIV, represented as percentage of WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. Statistical comparisons between groups were performed by one-way ANOVA followed by Newman-Keuls’ test. (* p

    Techniques Used: Inhibition, Western Blot, Expressing

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    Alomone Labs anti ncx1 slc8a1 antibody
    <t>NCX1</t> coordinates with TRPC1 to promote proliferation and migration of human GC cells. Summary data showing the inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (1 mM)-enhanced proliferation ( A – C ) and migration ( G – I ) of GC cells. D–F , J–L Summary data showing the inhibitory effect of either shNCX1, SKF96365 (SKF, 1 μM) or shNCX1 plus SKF on CaCl 2 (1 mM)-enhanced proliferation and migration of GC cells. Scale bar = 200 μm for each image. ( * P
    Anti Ncx1 Slc8a1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NCX1 coordinates with TRPC1 to promote proliferation and migration of human GC cells. Summary data showing the inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (1 mM)-enhanced proliferation ( A – C ) and migration ( G – I ) of GC cells. D–F , J–L Summary data showing the inhibitory effect of either shNCX1, SKF96365 (SKF, 1 μM) or shNCX1 plus SKF on CaCl 2 (1 mM)-enhanced proliferation and migration of GC cells. Scale bar = 200 μm for each image. ( * P

    Journal: Oncogene

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway

    doi: 10.1038/s41388-022-02412-9

    Figure Lengend Snippet: NCX1 coordinates with TRPC1 to promote proliferation and migration of human GC cells. Summary data showing the inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (1 mM)-enhanced proliferation ( A – C ) and migration ( G – I ) of GC cells. D–F , J–L Summary data showing the inhibitory effect of either shNCX1, SKF96365 (SKF, 1 μM) or shNCX1 plus SKF on CaCl 2 (1 mM)-enhanced proliferation and migration of GC cells. Scale bar = 200 μm for each image. ( * P

    Article Snippet: The antibodies of co-immunoprecipitation are anti-TRPC1 (No. ACC-010, alomone labs, Israel) and anti-NCX1 (No. ANX-011, alomone labs, Israel).

    Techniques: Migration

    TRPC1/NCX1 coupling induces AKT phosphorylation and promotes GC growth and metastasis. A , D Inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. B , E Inhibitory effects of SKF, shNCX1 or shNCX1 plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. C , F Inhibitory effects of either KB-R, shTRPC1 or shTRPC1 plus KB-R on CaCl 2 -induced AKT phosphorylation in GC cells. CaCl 2 promoted growth of xenografted gastric tumors ( G ), which was attenuated by either KB-R7943 ( H ) or shNCX1 ( I ). J Inhibitory effects of shNCX1 on CaCl 2 -induced gastric tumor metastasis. K Immunohistochemical analysis and histological examination on expression of NCX1 and Ki67 proteins with or without NCX1 knockdown in GC tissues. Scale bar = 100 μm for each image. L , M Summary data comparing expression of NCX1 and Ki67 proteins analyzed by immunohistochemistry between with or without NCX1 knockdown in GC tissues. ( * P

    Journal: Oncogene

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway

    doi: 10.1038/s41388-022-02412-9

    Figure Lengend Snippet: TRPC1/NCX1 coupling induces AKT phosphorylation and promotes GC growth and metastasis. A , D Inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. B , E Inhibitory effects of SKF, shNCX1 or shNCX1 plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. C , F Inhibitory effects of either KB-R, shTRPC1 or shTRPC1 plus KB-R on CaCl 2 -induced AKT phosphorylation in GC cells. CaCl 2 promoted growth of xenografted gastric tumors ( G ), which was attenuated by either KB-R7943 ( H ) or shNCX1 ( I ). J Inhibitory effects of shNCX1 on CaCl 2 -induced gastric tumor metastasis. K Immunohistochemical analysis and histological examination on expression of NCX1 and Ki67 proteins with or without NCX1 knockdown in GC tissues. Scale bar = 100 μm for each image. L , M Summary data comparing expression of NCX1 and Ki67 proteins analyzed by immunohistochemistry between with or without NCX1 knockdown in GC tissues. ( * P

    Article Snippet: The antibodies of co-immunoprecipitation are anti-TRPC1 (No. ACC-010, alomone labs, Israel) and anti-NCX1 (No. ANX-011, alomone labs, Israel).

    Techniques: Immunohistochemistry, Expressing

    Hp virulence factor and acid stimulate NCX1/TRPC1 coupling in human GC cells. Summary tracings of [Ca 2+ ] cyt time courses in response to extracellular 0 Na + ( A ), NH 4 Cl (5 mM) ( C ) and pH 4.5 ( E ) in the presence of extracellular 2 Ca 2+ or 0 Ca 2+ (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( A , n = 20 cells; C , n = 11 cells; E , n = 11 cells, **** P

    Journal: Oncogene

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway

    doi: 10.1038/s41388-022-02412-9

    Figure Lengend Snippet: Hp virulence factor and acid stimulate NCX1/TRPC1 coupling in human GC cells. Summary tracings of [Ca 2+ ] cyt time courses in response to extracellular 0 Na + ( A ), NH 4 Cl (5 mM) ( C ) and pH 4.5 ( E ) in the presence of extracellular 2 Ca 2+ or 0 Ca 2+ (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( A , n = 20 cells; C , n = 11 cells; E , n = 11 cells, **** P

    Article Snippet: The antibodies of co-immunoprecipitation are anti-TRPC1 (No. ACC-010, alomone labs, Israel) and anti-NCX1 (No. ANX-011, alomone labs, Israel).

    Techniques:

    NCX1 activation induces phosphorylation of AKT and β-catenin in human GC cells. A , B , E , F Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS) on CaCl 2 (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. C , D , G , H Inhibitory effect of KB-R7943 on NH 4 Cl (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. I , J , M , N Inhibitory effect of shNCX1 on CaCl 2 -induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. K , L , O , P Inhibitory effect of shNCX1 on NH 4 Cl-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. ( * P

    Journal: Oncogene

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway

    doi: 10.1038/s41388-022-02412-9

    Figure Lengend Snippet: NCX1 activation induces phosphorylation of AKT and β-catenin in human GC cells. A , B , E , F Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS) on CaCl 2 (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. C , D , G , H Inhibitory effect of KB-R7943 on NH 4 Cl (2 mM)-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. I , J , M , N Inhibitory effect of shNCX1 on CaCl 2 -induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. K , L , O , P Inhibitory effect of shNCX1 on NH 4 Cl-induced AKT and β-catenin phosphorylation in MKN45 and AGS cells. ( * P

    Article Snippet: The antibodies of co-immunoprecipitation are anti-TRPC1 (No. ACC-010, alomone labs, Israel) and anti-NCX1 (No. ANX-011, alomone labs, Israel).

    Techniques: Activation Assay

    The enhanced expression of NCX1 and TRPC1 in human primary gastric cancer tissues and cells. Western blot analysis applied to compare the expression levels of NCX1 proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P

    Journal: Oncogene

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway

    doi: 10.1038/s41388-022-02412-9

    Figure Lengend Snippet: The enhanced expression of NCX1 and TRPC1 in human primary gastric cancer tissues and cells. Western blot analysis applied to compare the expression levels of NCX1 proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P

    Article Snippet: The antibodies of co-immunoprecipitation are anti-TRPC1 (No. ACC-010, alomone labs, Israel) and anti-NCX1 (No. ANX-011, alomone labs, Israel).

    Techniques: Expressing, Western Blot, Staining, Negative Control

    NCX1 activation promotes proliferation and invasion of human GC cells. Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in MKN45 ( A ), AGS ( D ), and SGC7901 ( G ) cells. The inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) on CaCl 2 (1 mM)-induced proliferation ( B , E , H ) and invasion ( C , F , I ) of GC cells, Scale bar=200 μm for each image. Dose-dependently enhanced proliferation of NH 4 Cl (0.1-2 mM) in MKN45 ( J ), AGS ( L ), and SGC7901 ( N ) cells, and the inhibitory effect of KB-R7943 on NH 4 Cl (1 mM)-induced proliferation of MKN45 ( K ), AGS ( M ), and SGC7901 ( O ) cells. P , Q Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in CHO-NCX1 with NCX1 overexpression, and the inhibitory effect of KB-R7943 (0.2 μM) on CaCl 2 (1 mM)-induced proliferation of CHO-NCX1 cells. R–U No effects of CaCl 2 (0.1–2 mM) and NH 4 Cl (0.1–2 mM) on proliferation of CHO-K1 without NCX1 overexpression and GES1 cells. ( * P

    Journal: Oncogene

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway

    doi: 10.1038/s41388-022-02412-9

    Figure Lengend Snippet: NCX1 activation promotes proliferation and invasion of human GC cells. Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in MKN45 ( A ), AGS ( D ), and SGC7901 ( G ) cells. The inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) on CaCl 2 (1 mM)-induced proliferation ( B , E , H ) and invasion ( C , F , I ) of GC cells, Scale bar=200 μm for each image. Dose-dependently enhanced proliferation of NH 4 Cl (0.1-2 mM) in MKN45 ( J ), AGS ( L ), and SGC7901 ( N ) cells, and the inhibitory effect of KB-R7943 on NH 4 Cl (1 mM)-induced proliferation of MKN45 ( K ), AGS ( M ), and SGC7901 ( O ) cells. P , Q Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in CHO-NCX1 with NCX1 overexpression, and the inhibitory effect of KB-R7943 (0.2 μM) on CaCl 2 (1 mM)-induced proliferation of CHO-NCX1 cells. R–U No effects of CaCl 2 (0.1–2 mM) and NH 4 Cl (0.1–2 mM) on proliferation of CHO-K1 without NCX1 overexpression and GES1 cells. ( * P

    Article Snippet: The antibodies of co-immunoprecipitation are anti-TRPC1 (No. ACC-010, alomone labs, Israel) and anti-NCX1 (No. ANX-011, alomone labs, Israel).

    Techniques: Activation Assay, Over Expression

    CaCl 2 , Hp and virulence factors enhance NCX1 expression in human GC cells. A , G , M Representative time courses of CaCl 2 (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) ( B , H , N ) or shNCX1 ( C , I , O ) on CaCl 2 (2 mM)-enhanced NCX1 expression in GC cells. D , J , P Representative time courses of NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 ( E , K , Q ) or shNCX1 ( F , L , R ) on NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. S , T , U Representative time courses LPS (10 ng/ml)-enhanced NCX1 protein expression in GC cells. V , W Representative time courses H. pylori -enhanced NCX1 protein in GC cells. ( * P

    Journal: Oncogene

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway

    doi: 10.1038/s41388-022-02412-9

    Figure Lengend Snippet: CaCl 2 , Hp and virulence factors enhance NCX1 expression in human GC cells. A , G , M Representative time courses of CaCl 2 (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) ( B , H , N ) or shNCX1 ( C , I , O ) on CaCl 2 (2 mM)-enhanced NCX1 expression in GC cells. D , J , P Representative time courses of NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 ( E , K , Q ) or shNCX1 ( F , L , R ) on NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. S , T , U Representative time courses LPS (10 ng/ml)-enhanced NCX1 protein expression in GC cells. V , W Representative time courses H. pylori -enhanced NCX1 protein in GC cells. ( * P

    Article Snippet: The antibodies of co-immunoprecipitation are anti-TRPC1 (No. ACC-010, alomone labs, Israel) and anti-NCX1 (No. ANX-011, alomone labs, Israel).

    Techniques: Expressing

    CaCl 2 and NH 4 Cl promote proliferation and invasion of human GC cells through NCX1 activation. Representative images of NCX1 protein expression in GC cells with NCX1 knockdown and summary data of NCX1 protein levels in MKN45 ( A ), AGS ( B ), and SGC7901 ( C ) cells ( * P

    Journal: Oncogene

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway

    doi: 10.1038/s41388-022-02412-9

    Figure Lengend Snippet: CaCl 2 and NH 4 Cl promote proliferation and invasion of human GC cells through NCX1 activation. Representative images of NCX1 protein expression in GC cells with NCX1 knockdown and summary data of NCX1 protein levels in MKN45 ( A ), AGS ( B ), and SGC7901 ( C ) cells ( * P

    Article Snippet: The antibodies of co-immunoprecipitation are anti-TRPC1 (No. ACC-010, alomone labs, Israel) and anti-NCX1 (No. ANX-011, alomone labs, Israel).

    Techniques: Activation Assay, Expressing

    Changed expression of the Na,K‐ATPase α isoforms in the heart from α 2 +/G301R mice but similar expression of the Na,Ca‐exchanger‐1. The hearts from 3‐ and 8‐month‐old α 2 +/G301R mice showed reduced expression of the Na,K‐ATPase α 2 isoform ( A , n=7; D , n=6), increased expression of Na,K‐ATPase α 1 isoform ( B , n=7; E , n=5 to 6), and no change in the expression of Na,Ca‐exchager‐1 (NCX1; C , n=7; F , n=12) in comparison with wild type (WT). The expression profile was the same in hearts of 3‐month‐old ( A – C ) and 8‐month‐old ( D – F ) mice. Upper part of each panel shows representative Western blot bands for the averaged data shown below. The representative bands are shown from the same membrane, as well as the molecular marker shown to the right. The images cropped to include molecular weights from 250 to 50 kDa, as indicated. Total protein load was detected with the stain‐free gel, and the corresponding representative bands were cropped to the same molecular weight range. Protein expression was compared using unpaired t ‐test. * P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Migraine‐Associated Mutation in the Na,K‐ATPase Leads to Disturbances in Cardiac Metabolism and Reduced Cardiac Function

    doi: 10.1161/JAHA.121.021814

    Figure Lengend Snippet: Changed expression of the Na,K‐ATPase α isoforms in the heart from α 2 +/G301R mice but similar expression of the Na,Ca‐exchanger‐1. The hearts from 3‐ and 8‐month‐old α 2 +/G301R mice showed reduced expression of the Na,K‐ATPase α 2 isoform ( A , n=7; D , n=6), increased expression of Na,K‐ATPase α 1 isoform ( B , n=7; E , n=5 to 6), and no change in the expression of Na,Ca‐exchager‐1 (NCX1; C , n=7; F , n=12) in comparison with wild type (WT). The expression profile was the same in hearts of 3‐month‐old ( A – C ) and 8‐month‐old ( D – F ) mice. Upper part of each panel shows representative Western blot bands for the averaged data shown below. The representative bands are shown from the same membrane, as well as the molecular marker shown to the right. The images cropped to include molecular weights from 250 to 50 kDa, as indicated. Total protein load was detected with the stain‐free gel, and the corresponding representative bands were cropped to the same molecular weight range. Protein expression was compared using unpaired t ‐test. * P

    Article Snippet: Antibody against the Na,Ca‐exchanger‐1 (1:200; No. ANX‐011; Alomone Labs, Israel).

    Techniques: Expressing, Mouse Assay, Western Blot, Marker, Staining, Molecular Weight

    Effect of NCX3 silencing or inhibition by KB-R7943 in WT and Tg2576 primary hippocampal neurons. (A) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded in WT and WT plus siNCX3 (black traces), Tg2576 and Tg2576 plus siNCX3 (grey traces) primary hippocampal neurons at 12 DIV. (B) Quantification of I NCX in the reverse mode of operation represented in A, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. (C) Representative Western blotting experiments and relative quantifications showing the effect of NCX3 silencing (siNCX3) on NCX3, NCX1, and NCX2 protein expression in primary hippocampal neurons (D) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded from WT and WT plus 0.5 μM kB-R7943 (black traces), Tg2576 and Tg2576 plus 0.5 μM kB-R7943 (grey traces) primary hippocampal neurons at 12 DIV. (E) Quantification of I NCX in the reverse mode of operation represented in D, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. The number of cells used for each experimental condition is noted on the bars. (F, G) Representative Western blot of NCX3 protein expression and densitometric quantification of NCX3 truncated band in WT and Tg2576 primary hippocampal neurons at 12 DIV, represented as percentage of WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. Statistical comparisons between groups were performed by one-way ANOVA followed by Newman-Keuls’ test. (* p

    Journal: Frontiers in Pharmacology

    Article Title: The Na+/Ca2+ Exchanger 3 Is Functionally Coupled With the NaV1.6 Voltage-Gated Channel and Promotes an Endoplasmic Reticulum Ca2+ Refilling in a Transgenic Model of Alzheimer’s Disease

    doi: 10.3389/fphar.2021.775271

    Figure Lengend Snippet: Effect of NCX3 silencing or inhibition by KB-R7943 in WT and Tg2576 primary hippocampal neurons. (A) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded in WT and WT plus siNCX3 (black traces), Tg2576 and Tg2576 plus siNCX3 (grey traces) primary hippocampal neurons at 12 DIV. (B) Quantification of I NCX in the reverse mode of operation represented in A, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. (C) Representative Western blotting experiments and relative quantifications showing the effect of NCX3 silencing (siNCX3) on NCX3, NCX1, and NCX2 protein expression in primary hippocampal neurons (D) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded from WT and WT plus 0.5 μM kB-R7943 (black traces), Tg2576 and Tg2576 plus 0.5 μM kB-R7943 (grey traces) primary hippocampal neurons at 12 DIV. (E) Quantification of I NCX in the reverse mode of operation represented in D, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. The number of cells used for each experimental condition is noted on the bars. (F, G) Representative Western blot of NCX3 protein expression and densitometric quantification of NCX3 truncated band in WT and Tg2576 primary hippocampal neurons at 12 DIV, represented as percentage of WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. Statistical comparisons between groups were performed by one-way ANOVA followed by Newman-Keuls’ test. (* p

    Article Snippet: The nitrocellulose membranes were incubated with the following antibodies: rabbit-polyclonal anti-NCX3, anti-NCX1, anti-NCX2 (1:1,000, Alomone Labs, Israel) and anti-β-actin peroxidase (1:10,000, Sigma-Aldrich, Milan, Italy).

    Techniques: Inhibition, Western Blot, Expressing