anti ncx1  (Alomone Labs)


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    Alomone Labs anti ncx1
    Anti Ncx1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti ncx1  (Alomone Labs)


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    Alomone Labs anti ncx1
    Anti Ncx1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ncx1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    anti ncx1  (Alomone Labs)


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    Alomone Labs anti ncx1
    Western blot analysis applied to compare the expression levels of <t>NCX1</t> proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P < 0.0001, n = 80 patients). Relative <t>NCX1</t> <t>protein</t> levels in GC tissues from the patients with different stages ( G ), tumor sizes ( H ), and lymphatic metastasis ( I ). ( * P < 0.05, n = 80 patients). J Kaplan–Meier analysis of survival ratio of GC patients with low and high NCX1 expression levels ( * P < 0.05, n = 80 patients). K , L Western blot analysis of NCX1 and TRPC1 protein levels in GES1 and GC cell lines. M Immunofluoresence staining images of NCX1 and TRPC1 proteins with primary antibody and without the antibody (negative control) in MKN45, AGS and SGC7901 cells. Scale bar=10 μm for each image. N–Q Co-immunoprecipitation showing the binding of NCX1 and TRPC1 proteins in GC cells.
    Anti Ncx1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ncx1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ncx1 - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "NCX1 coupled with TRPC1 to promote gastric cancer via Ca 2+ /AKT/β-catenin pathway"

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca 2+ /AKT/β-catenin pathway

    Journal: Oncogene

    doi: 10.1038/s41388-022-02412-9

    Western blot analysis applied to compare the expression levels of NCX1 proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P < 0.0001, n = 80 patients). Relative NCX1 protein levels in GC tissues from the patients with different stages ( G ), tumor sizes ( H ), and lymphatic metastasis ( I ). ( * P < 0.05, n = 80 patients). J Kaplan–Meier analysis of survival ratio of GC patients with low and high NCX1 expression levels ( * P < 0.05, n = 80 patients). K , L Western blot analysis of NCX1 and TRPC1 protein levels in GES1 and GC cell lines. M Immunofluoresence staining images of NCX1 and TRPC1 proteins with primary antibody and without the antibody (negative control) in MKN45, AGS and SGC7901 cells. Scale bar=10 μm for each image. N–Q Co-immunoprecipitation showing the binding of NCX1 and TRPC1 proteins in GC cells.
    Figure Legend Snippet: Western blot analysis applied to compare the expression levels of NCX1 proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P < 0.0001, n = 80 patients). Relative NCX1 protein levels in GC tissues from the patients with different stages ( G ), tumor sizes ( H ), and lymphatic metastasis ( I ). ( * P < 0.05, n = 80 patients). J Kaplan–Meier analysis of survival ratio of GC patients with low and high NCX1 expression levels ( * P < 0.05, n = 80 patients). K , L Western blot analysis of NCX1 and TRPC1 protein levels in GES1 and GC cell lines. M Immunofluoresence staining images of NCX1 and TRPC1 proteins with primary antibody and without the antibody (negative control) in MKN45, AGS and SGC7901 cells. Scale bar=10 μm for each image. N–Q Co-immunoprecipitation showing the binding of NCX1 and TRPC1 proteins in GC cells.

    Techniques Used: Western Blot, Expressing, Staining, Negative Control, Immunoprecipitation, Binding Assay

    Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in MKN45 ( A ), AGS ( D ), and SGC7901 ( G ) cells. The inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) on CaCl 2 (1 mM)-induced proliferation ( B , E , H ) and invasion ( C , F , I ) of GC cells, Scale bar=200 μm for each image. Dose-dependently enhanced proliferation of NH 4 Cl (0.1-2 mM) in MKN45 ( J ), AGS ( L ), and SGC7901 ( N ) cells, and the inhibitory effect of KB-R7943 on NH 4 Cl (1 mM)-induced proliferation of MKN45 ( K ), AGS ( M ), and SGC7901 ( O ) cells. P , Q Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in CHO-NCX1 with NCX1 overexpression, and the inhibitory effect of KB-R7943 (0.2 μM) on CaCl 2 (1 mM)-induced proliferation of CHO-NCX1 cells. R–U No effects of CaCl 2 (0.1–2 mM) and NH 4 Cl (0.1–2 mM) on proliferation of CHO-K1 without NCX1 overexpression and GES1 cells. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences).
    Figure Legend Snippet: Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in MKN45 ( A ), AGS ( D ), and SGC7901 ( G ) cells. The inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) on CaCl 2 (1 mM)-induced proliferation ( B , E , H ) and invasion ( C , F , I ) of GC cells, Scale bar=200 μm for each image. Dose-dependently enhanced proliferation of NH 4 Cl (0.1-2 mM) in MKN45 ( J ), AGS ( L ), and SGC7901 ( N ) cells, and the inhibitory effect of KB-R7943 on NH 4 Cl (1 mM)-induced proliferation of MKN45 ( K ), AGS ( M ), and SGC7901 ( O ) cells. P , Q Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in CHO-NCX1 with NCX1 overexpression, and the inhibitory effect of KB-R7943 (0.2 μM) on CaCl 2 (1 mM)-induced proliferation of CHO-NCX1 cells. R–U No effects of CaCl 2 (0.1–2 mM) and NH 4 Cl (0.1–2 mM) on proliferation of CHO-K1 without NCX1 overexpression and GES1 cells. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences).

    Techniques Used: Over Expression

    Representative images of NCX1 protein expression in GC cells with NCX1 knockdown and summary data of NCX1 protein levels in MKN45 ( A ), AGS ( B ), and SGC7901 ( C ) cells ( * P < 0.05, ** P < 0.01, *** P < 0.001, vs . NC, n = 3). The effect of shNCX1 on CaCl 2 (1 mM)-induced proliferation ( D , F , H ) and invasion ( E , G , I ) of GC cells. The effect of shNCX1 on NH 4 Cl (1 mM)-induced proliferation ( J , L , N ) and invasion ( K , M , O ) of GC cells. Scale bar = 200 μm for each image. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences).
    Figure Legend Snippet: Representative images of NCX1 protein expression in GC cells with NCX1 knockdown and summary data of NCX1 protein levels in MKN45 ( A ), AGS ( B ), and SGC7901 ( C ) cells ( * P < 0.05, ** P < 0.01, *** P < 0.001, vs . NC, n = 3). The effect of shNCX1 on CaCl 2 (1 mM)-induced proliferation ( D , F , H ) and invasion ( E , G , I ) of GC cells. The effect of shNCX1 on NH 4 Cl (1 mM)-induced proliferation ( J , L , N ) and invasion ( K , M , O ) of GC cells. Scale bar = 200 μm for each image. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences).

    Techniques Used: Expressing

    A , G , M Representative time courses of CaCl 2 (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) ( B , H , N ) or shNCX1 ( C , I , O ) on CaCl 2 (2 mM)-enhanced NCX1 expression in GC cells. D , J , P Representative time courses of NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 ( E , K , Q ) or shNCX1 ( F , L , R ) on NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. S , T , U Representative time courses LPS (10 ng/ml)-enhanced NCX1 protein expression in GC cells. V , W Representative time courses H. pylori -enhanced NCX1 protein in GC cells. ( * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3; ns, no significant differences).
    Figure Legend Snippet: A , G , M Representative time courses of CaCl 2 (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) ( B , H , N ) or shNCX1 ( C , I , O ) on CaCl 2 (2 mM)-enhanced NCX1 expression in GC cells. D , J , P Representative time courses of NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 ( E , K , Q ) or shNCX1 ( F , L , R ) on NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. S , T , U Representative time courses LPS (10 ng/ml)-enhanced NCX1 protein expression in GC cells. V , W Representative time courses H. pylori -enhanced NCX1 protein in GC cells. ( * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3; ns, no significant differences).

    Techniques Used: Expressing

    Summary tracings of [Ca 2+ ] cyt time courses in response to extracellular 0 Na + ( A ), NH 4 Cl (5 mM) ( C ) and pH 4.5 ( E ) in the presence of extracellular 2 Ca 2+ or 0 Ca 2+ (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( A , n = 20 cells; C , n = 11 cells; E , n = 11 cells, **** P < 0.0001). Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + ( B ), NH 4 Cl (5 mM) ( D ) and pH 4.5 ( F) in the presence of 2 Ca 2+ or 2 Ca 2+ plus KB-R7943 (KB-R, 30 μM) (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( B , n = 20 cells; D , n = 23 cells; F , n = 26 cells, **** P < 0.0001). Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + in the presence of 2 Ca 2+ or 5Ca 2+ in CHO-NCX1 ( G ) and CHO-K1 ( H ) cells. I Summary data showing the peaks of 0 Na + -increased [Ca 2+ ] cyt signaling described as in G , H ( G , n = 23 cells; H , n = 17 cells, **** P < 0.0001). J Western blot analysis of NCX1 protein levels in CHO-NCX1 and CHO-K1 cells. Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + ( K , L ) and NH 4 Cl (5 mM) ( O , P ) in the presence of 2 Ca 2+ in NC ( K , O ) or shTRPC1 ( L , P ) of SGC7901 cells. Summary data showing the peaks of 0 Na + ( M ) or NH 4 Cl ( Q )-increased [Ca 2+ ] cyt signaling described as in K , L , O , P ( n = 11 cells, **** P < 0.0001). N , R RT-PCR analysis of TRPC1 mRNA levels in NC and shTRPC1 of GC cells ( n = 3, * P < 0.05, **** P < 0.0001). Each one is 3 independent experiments with similar results.
    Figure Legend Snippet: Summary tracings of [Ca 2+ ] cyt time courses in response to extracellular 0 Na + ( A ), NH 4 Cl (5 mM) ( C ) and pH 4.5 ( E ) in the presence of extracellular 2 Ca 2+ or 0 Ca 2+ (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( A , n = 20 cells; C , n = 11 cells; E , n = 11 cells, **** P < 0.0001). Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + ( B ), NH 4 Cl (5 mM) ( D ) and pH 4.5 ( F) in the presence of 2 Ca 2+ or 2 Ca 2+ plus KB-R7943 (KB-R, 30 μM) (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( B , n = 20 cells; D , n = 23 cells; F , n = 26 cells, **** P < 0.0001). Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + in the presence of 2 Ca 2+ or 5Ca 2+ in CHO-NCX1 ( G ) and CHO-K1 ( H ) cells. I Summary data showing the peaks of 0 Na + -increased [Ca 2+ ] cyt signaling described as in G , H ( G , n = 23 cells; H , n = 17 cells, **** P < 0.0001). J Western blot analysis of NCX1 protein levels in CHO-NCX1 and CHO-K1 cells. Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + ( K , L ) and NH 4 Cl (5 mM) ( O , P ) in the presence of 2 Ca 2+ in NC ( K , O ) or shTRPC1 ( L , P ) of SGC7901 cells. Summary data showing the peaks of 0 Na + ( M ) or NH 4 Cl ( Q )-increased [Ca 2+ ] cyt signaling described as in K , L , O , P ( n = 11 cells, **** P < 0.0001). N , R RT-PCR analysis of TRPC1 mRNA levels in NC and shTRPC1 of GC cells ( n = 3, * P < 0.05, **** P < 0.0001). Each one is 3 independent experiments with similar results.

    Techniques Used: Western Blot, Reverse Transcription Polymerase Chain Reaction

    A , D Inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. B , E Inhibitory effects of SKF, shNCX1 or shNCX1 plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. C , F Inhibitory effects of either KB-R, shTRPC1 or shTRPC1 plus KB-R on CaCl 2 -induced AKT phosphorylation in GC cells. CaCl 2 promoted growth of xenografted gastric tumors ( G ), which was attenuated by either KB-R7943 ( H ) or shNCX1 ( I ). J Inhibitory effects of shNCX1 on CaCl 2 -induced gastric tumor metastasis. K Immunohistochemical analysis and histological examination on expression of NCX1 and Ki67 proteins with or without NCX1 knockdown in GC tissues. Scale bar = 100 μm for each image. L , M Summary data comparing expression of NCX1 and Ki67 proteins analyzed by immunohistochemistry between with or without NCX1 knockdown in GC tissues. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences). N The proposed oncogenic mechanisms of TRPC1/NCX1 coupling via Ca 2+ /AKT/β-catenin pathway in Hp -associated GC.
    Figure Legend Snippet: A , D Inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. B , E Inhibitory effects of SKF, shNCX1 or shNCX1 plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. C , F Inhibitory effects of either KB-R, shTRPC1 or shTRPC1 plus KB-R on CaCl 2 -induced AKT phosphorylation in GC cells. CaCl 2 promoted growth of xenografted gastric tumors ( G ), which was attenuated by either KB-R7943 ( H ) or shNCX1 ( I ). J Inhibitory effects of shNCX1 on CaCl 2 -induced gastric tumor metastasis. K Immunohistochemical analysis and histological examination on expression of NCX1 and Ki67 proteins with or without NCX1 knockdown in GC tissues. Scale bar = 100 μm for each image. L , M Summary data comparing expression of NCX1 and Ki67 proteins analyzed by immunohistochemistry between with or without NCX1 knockdown in GC tissues. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences). N The proposed oncogenic mechanisms of TRPC1/NCX1 coupling via Ca 2+ /AKT/β-catenin pathway in Hp -associated GC.

    Techniques Used: Immunohistochemical staining, Expressing, Immunohistochemistry

    anti ncx1  (Alomone Labs)


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    Alomone Labs anti ncx1
    Western blot analysis applied to compare the expression levels of <t>NCX1</t> proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P < 0.0001, n = 80 patients). Relative <t>NCX1</t> <t>protein</t> levels in GC tissues from the patients with different stages ( G ), tumor sizes ( H ), and lymphatic metastasis ( I ). ( * P < 0.05, n = 80 patients). J Kaplan–Meier analysis of survival ratio of GC patients with low and high NCX1 expression levels ( * P < 0.05, n = 80 patients). K , L Western blot analysis of NCX1 and TRPC1 protein levels in GES1 and GC cell lines. M Immunofluoresence staining images of NCX1 and TRPC1 proteins with primary antibody and without the antibody (negative control) in MKN45, AGS and SGC7901 cells. Scale bar=10 μm for each image. N–Q Co-immunoprecipitation showing the binding of NCX1 and TRPC1 proteins in GC cells.
    Anti Ncx1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ncx1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ncx1 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "NCX1 coupled with TRPC1 to promote gastric cancer via Ca 2+ /AKT/β-catenin pathway"

    Article Title: NCX1 coupled with TRPC1 to promote gastric cancer via Ca 2+ /AKT/β-catenin pathway

    Journal: Oncogene

    doi: 10.1038/s41388-022-02412-9

    Western blot analysis applied to compare the expression levels of NCX1 proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P < 0.0001, n = 80 patients). Relative NCX1 protein levels in GC tissues from the patients with different stages ( G ), tumor sizes ( H ), and lymphatic metastasis ( I ). ( * P < 0.05, n = 80 patients). J Kaplan–Meier analysis of survival ratio of GC patients with low and high NCX1 expression levels ( * P < 0.05, n = 80 patients). K , L Western blot analysis of NCX1 and TRPC1 protein levels in GES1 and GC cell lines. M Immunofluoresence staining images of NCX1 and TRPC1 proteins with primary antibody and without the antibody (negative control) in MKN45, AGS and SGC7901 cells. Scale bar=10 μm for each image. N–Q Co-immunoprecipitation showing the binding of NCX1 and TRPC1 proteins in GC cells.
    Figure Legend Snippet: Western blot analysis applied to compare the expression levels of NCX1 proteins between gastric cancer (GC) tissues and adjacent normal (Nor) tissues from 52 GC patients: 34 pairs with high expression ( A ), 13 pairs with low expression ( B ), and 5 pairs without difference ( C ). D Summary data showing the percentage of high, low and indifference of NCX1 expression in GC tissues compared to adjacent tissues. E , F Representative and summary data of immunohistological staining on NCX1 proteins in GC tissues compared to adjacent tissues. Scale bar=100 μm for each image. Negative control: without primary antibody. ( **** P < 0.0001, n = 80 patients). Relative NCX1 protein levels in GC tissues from the patients with different stages ( G ), tumor sizes ( H ), and lymphatic metastasis ( I ). ( * P < 0.05, n = 80 patients). J Kaplan–Meier analysis of survival ratio of GC patients with low and high NCX1 expression levels ( * P < 0.05, n = 80 patients). K , L Western blot analysis of NCX1 and TRPC1 protein levels in GES1 and GC cell lines. M Immunofluoresence staining images of NCX1 and TRPC1 proteins with primary antibody and without the antibody (negative control) in MKN45, AGS and SGC7901 cells. Scale bar=10 μm for each image. N–Q Co-immunoprecipitation showing the binding of NCX1 and TRPC1 proteins in GC cells.

    Techniques Used: Western Blot, Expressing, Staining, Negative Control, Immunoprecipitation, Binding Assay

    Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in MKN45 ( A ), AGS ( D ), and SGC7901 ( G ) cells. The inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) on CaCl 2 (1 mM)-induced proliferation ( B , E , H ) and invasion ( C , F , I ) of GC cells, Scale bar=200 μm for each image. Dose-dependently enhanced proliferation of NH 4 Cl (0.1-2 mM) in MKN45 ( J ), AGS ( L ), and SGC7901 ( N ) cells, and the inhibitory effect of KB-R7943 on NH 4 Cl (1 mM)-induced proliferation of MKN45 ( K ), AGS ( M ), and SGC7901 ( O ) cells. P , Q Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in CHO-NCX1 with NCX1 overexpression, and the inhibitory effect of KB-R7943 (0.2 μM) on CaCl 2 (1 mM)-induced proliferation of CHO-NCX1 cells. R–U No effects of CaCl 2 (0.1–2 mM) and NH 4 Cl (0.1–2 mM) on proliferation of CHO-K1 without NCX1 overexpression and GES1 cells. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences).
    Figure Legend Snippet: Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in MKN45 ( A ), AGS ( D ), and SGC7901 ( G ) cells. The inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) on CaCl 2 (1 mM)-induced proliferation ( B , E , H ) and invasion ( C , F , I ) of GC cells, Scale bar=200 μm for each image. Dose-dependently enhanced proliferation of NH 4 Cl (0.1-2 mM) in MKN45 ( J ), AGS ( L ), and SGC7901 ( N ) cells, and the inhibitory effect of KB-R7943 on NH 4 Cl (1 mM)-induced proliferation of MKN45 ( K ), AGS ( M ), and SGC7901 ( O ) cells. P , Q Dose-dependently enhanced proliferation of CaCl 2 (0.1-2 mM) in CHO-NCX1 with NCX1 overexpression, and the inhibitory effect of KB-R7943 (0.2 μM) on CaCl 2 (1 mM)-induced proliferation of CHO-NCX1 cells. R–U No effects of CaCl 2 (0.1–2 mM) and NH 4 Cl (0.1–2 mM) on proliferation of CHO-K1 without NCX1 overexpression and GES1 cells. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences).

    Techniques Used: Over Expression

    Representative images of NCX1 protein expression in GC cells with NCX1 knockdown and summary data of NCX1 protein levels in MKN45 ( A ), AGS ( B ), and SGC7901 ( C ) cells ( * P < 0.05, ** P < 0.01, *** P < 0.001, vs . NC, n = 3). The effect of shNCX1 on CaCl 2 (1 mM)-induced proliferation ( D , F , H ) and invasion ( E , G , I ) of GC cells. The effect of shNCX1 on NH 4 Cl (1 mM)-induced proliferation ( J , L , N ) and invasion ( K , M , O ) of GC cells. Scale bar = 200 μm for each image. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences).
    Figure Legend Snippet: Representative images of NCX1 protein expression in GC cells with NCX1 knockdown and summary data of NCX1 protein levels in MKN45 ( A ), AGS ( B ), and SGC7901 ( C ) cells ( * P < 0.05, ** P < 0.01, *** P < 0.001, vs . NC, n = 3). The effect of shNCX1 on CaCl 2 (1 mM)-induced proliferation ( D , F , H ) and invasion ( E , G , I ) of GC cells. The effect of shNCX1 on NH 4 Cl (1 mM)-induced proliferation ( J , L , N ) and invasion ( K , M , O ) of GC cells. Scale bar = 200 μm for each image. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences).

    Techniques Used: Expressing

    A , G , M Representative time courses of CaCl 2 (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) ( B , H , N ) or shNCX1 ( C , I , O ) on CaCl 2 (2 mM)-enhanced NCX1 expression in GC cells. D , J , P Representative time courses of NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 ( E , K , Q ) or shNCX1 ( F , L , R ) on NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. S , T , U Representative time courses LPS (10 ng/ml)-enhanced NCX1 protein expression in GC cells. V , W Representative time courses H. pylori -enhanced NCX1 protein in GC cells. ( * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3; ns, no significant differences).
    Figure Legend Snippet: A , G , M Representative time courses of CaCl 2 (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 (KB-R, 1 μM in MKN45, 4 μM in AGS, 8 μM in SGC7901) ( B , H , N ) or shNCX1 ( C , I , O ) on CaCl 2 (2 mM)-enhanced NCX1 expression in GC cells. D , J , P Representative time courses of NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. Inhibitory effect of KB-R7943 ( E , K , Q ) or shNCX1 ( F , L , R ) on NH 4 Cl (2 mM)-enhanced NCX1 protein expression in GC cells. S , T , U Representative time courses LPS (10 ng/ml)-enhanced NCX1 protein expression in GC cells. V , W Representative time courses H. pylori -enhanced NCX1 protein in GC cells. ( * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3; ns, no significant differences).

    Techniques Used: Expressing

    Summary tracings of [Ca 2+ ] cyt time courses in response to extracellular 0 Na + ( A ), NH 4 Cl (5 mM) ( C ) and pH 4.5 ( E ) in the presence of extracellular 2 Ca 2+ or 0 Ca 2+ (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( A , n = 20 cells; C , n = 11 cells; E , n = 11 cells, **** P < 0.0001). Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + ( B ), NH 4 Cl (5 mM) ( D ) and pH 4.5 ( F) in the presence of 2 Ca 2+ or 2 Ca 2+ plus KB-R7943 (KB-R, 30 μM) (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( B , n = 20 cells; D , n = 23 cells; F , n = 26 cells, **** P < 0.0001). Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + in the presence of 2 Ca 2+ or 5Ca 2+ in CHO-NCX1 ( G ) and CHO-K1 ( H ) cells. I Summary data showing the peaks of 0 Na + -increased [Ca 2+ ] cyt signaling described as in G , H ( G , n = 23 cells; H , n = 17 cells, **** P < 0.0001). J Western blot analysis of NCX1 protein levels in CHO-NCX1 and CHO-K1 cells. Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + ( K , L ) and NH 4 Cl (5 mM) ( O , P ) in the presence of 2 Ca 2+ in NC ( K , O ) or shTRPC1 ( L , P ) of SGC7901 cells. Summary data showing the peaks of 0 Na + ( M ) or NH 4 Cl ( Q )-increased [Ca 2+ ] cyt signaling described as in K , L , O , P ( n = 11 cells, **** P < 0.0001). N , R RT-PCR analysis of TRPC1 mRNA levels in NC and shTRPC1 of GC cells ( n = 3, * P < 0.05, **** P < 0.0001). Each one is 3 independent experiments with similar results.
    Figure Legend Snippet: Summary tracings of [Ca 2+ ] cyt time courses in response to extracellular 0 Na + ( A ), NH 4 Cl (5 mM) ( C ) and pH 4.5 ( E ) in the presence of extracellular 2 Ca 2+ or 0 Ca 2+ (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( A , n = 20 cells; C , n = 11 cells; E , n = 11 cells, **** P < 0.0001). Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + ( B ), NH 4 Cl (5 mM) ( D ) and pH 4.5 ( F) in the presence of 2 Ca 2+ or 2 Ca 2+ plus KB-R7943 (KB-R, 30 μM) (left). Summary data showing the peaks of 0 Na + , NH 4 Cl and pH 4.5-increased [Ca 2+ ] cyt signaling in SGC7901 cells (right) ( B , n = 20 cells; D , n = 23 cells; F , n = 26 cells, **** P < 0.0001). Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + in the presence of 2 Ca 2+ or 5Ca 2+ in CHO-NCX1 ( G ) and CHO-K1 ( H ) cells. I Summary data showing the peaks of 0 Na + -increased [Ca 2+ ] cyt signaling described as in G , H ( G , n = 23 cells; H , n = 17 cells, **** P < 0.0001). J Western blot analysis of NCX1 protein levels in CHO-NCX1 and CHO-K1 cells. Summary tracings of [Ca 2+ ] cyt time courses in response to 0 Na + ( K , L ) and NH 4 Cl (5 mM) ( O , P ) in the presence of 2 Ca 2+ in NC ( K , O ) or shTRPC1 ( L , P ) of SGC7901 cells. Summary data showing the peaks of 0 Na + ( M ) or NH 4 Cl ( Q )-increased [Ca 2+ ] cyt signaling described as in K , L , O , P ( n = 11 cells, **** P < 0.0001). N , R RT-PCR analysis of TRPC1 mRNA levels in NC and shTRPC1 of GC cells ( n = 3, * P < 0.05, **** P < 0.0001). Each one is 3 independent experiments with similar results.

    Techniques Used: Western Blot, Reverse Transcription Polymerase Chain Reaction

    A , D Inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. B , E Inhibitory effects of SKF, shNCX1 or shNCX1 plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. C , F Inhibitory effects of either KB-R, shTRPC1 or shTRPC1 plus KB-R on CaCl 2 -induced AKT phosphorylation in GC cells. CaCl 2 promoted growth of xenografted gastric tumors ( G ), which was attenuated by either KB-R7943 ( H ) or shNCX1 ( I ). J Inhibitory effects of shNCX1 on CaCl 2 -induced gastric tumor metastasis. K Immunohistochemical analysis and histological examination on expression of NCX1 and Ki67 proteins with or without NCX1 knockdown in GC tissues. Scale bar = 100 μm for each image. L , M Summary data comparing expression of NCX1 and Ki67 proteins analyzed by immunohistochemistry between with or without NCX1 knockdown in GC tissues. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences). N The proposed oncogenic mechanisms of TRPC1/NCX1 coupling via Ca 2+ /AKT/β-catenin pathway in Hp -associated GC.
    Figure Legend Snippet: A , D Inhibitory effects of either KB-R7943 (KB-R, 1 μM in MKN45, 8 μM in SGC7901), SKF96365 (SKF, 1 μM) or KB-R plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. B , E Inhibitory effects of SKF, shNCX1 or shNCX1 plus SKF on CaCl 2 (2 mM)-induced AKT phosphorylation in GC cells. C , F Inhibitory effects of either KB-R, shTRPC1 or shTRPC1 plus KB-R on CaCl 2 -induced AKT phosphorylation in GC cells. CaCl 2 promoted growth of xenografted gastric tumors ( G ), which was attenuated by either KB-R7943 ( H ) or shNCX1 ( I ). J Inhibitory effects of shNCX1 on CaCl 2 -induced gastric tumor metastasis. K Immunohistochemical analysis and histological examination on expression of NCX1 and Ki67 proteins with or without NCX1 knockdown in GC tissues. Scale bar = 100 μm for each image. L , M Summary data comparing expression of NCX1 and Ki67 proteins analyzed by immunohistochemistry between with or without NCX1 knockdown in GC tissues. ( * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3; ns, no significant differences). N The proposed oncogenic mechanisms of TRPC1/NCX1 coupling via Ca 2+ /AKT/β-catenin pathway in Hp -associated GC.

    Techniques Used: Immunohistochemical staining, Expressing, Immunohistochemistry

    anti ncx1  (Alomone Labs)


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    Alomone Labs anti ncx1
    Gene expression of ion channels, transporters, connexins, adrenergic and cholinergic receptors in cardiac pacemaker cells. Abundance of mRNA transcripts (in%) relative to gene expression of the housekeeping genes GAPDH/HPRT1/ACTB . ( A – C ): HCN channels. ( D ): Na-Ca exchanger <t>NCX1</t> . ( E – G ): calcium channels. ( H ): sodium channel SCN5A . ( I – K ): potassium channels. ( L–N ): connexins. ( O – R ): adrenergic and cholinergic receptors. Data are provided as means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, Diff. prot. A–E versus hRA. # p < 0.05, ## p < 0.01, ### p < 0.001, Diff. prot. B–E versus Diff. prot. A. Abbreviations: hRA: human right atrium, hSAN: human sinoatrial node, Diff. prot. = differentiation protocol.
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    1) Product Images from "Improved Generation of Human Induced Pluripotent Stem Cell-Derived Cardiac Pacemaker Cells Using Novel Differentiation Protocols"

    Article Title: Improved Generation of Human Induced Pluripotent Stem Cell-Derived Cardiac Pacemaker Cells Using Novel Differentiation Protocols

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23137318

    Gene expression of ion channels, transporters, connexins, adrenergic and cholinergic receptors in cardiac pacemaker cells. Abundance of mRNA transcripts (in%) relative to gene expression of the housekeeping genes GAPDH/HPRT1/ACTB . ( A – C ): HCN channels. ( D ): Na-Ca exchanger NCX1 . ( E – G ): calcium channels. ( H ): sodium channel SCN5A . ( I – K ): potassium channels. ( L–N ): connexins. ( O – R ): adrenergic and cholinergic receptors. Data are provided as means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, Diff. prot. A–E versus hRA. # p < 0.05, ## p < 0.01, ### p < 0.001, Diff. prot. B–E versus Diff. prot. A. Abbreviations: hRA: human right atrium, hSAN: human sinoatrial node, Diff. prot. = differentiation protocol.
    Figure Legend Snippet: Gene expression of ion channels, transporters, connexins, adrenergic and cholinergic receptors in cardiac pacemaker cells. Abundance of mRNA transcripts (in%) relative to gene expression of the housekeeping genes GAPDH/HPRT1/ACTB . ( A – C ): HCN channels. ( D ): Na-Ca exchanger NCX1 . ( E – G ): calcium channels. ( H ): sodium channel SCN5A . ( I – K ): potassium channels. ( L–N ): connexins. ( O – R ): adrenergic and cholinergic receptors. Data are provided as means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, Diff. prot. A–E versus hRA. # p < 0.05, ## p < 0.01, ### p < 0.001, Diff. prot. B–E versus Diff. prot. A. Abbreviations: hRA: human right atrium, hSAN: human sinoatrial node, Diff. prot. = differentiation protocol.

    Techniques Used: Expressing

    Assessment of pacemaker-specific gene expression in cardiac pacemaker cells.
    Figure Legend Snippet: Assessment of pacemaker-specific gene expression in cardiac pacemaker cells.

    Techniques Used: Expressing

    Overview of the used TaqMan probes and primers.
    Figure Legend Snippet: Overview of the used TaqMan probes and primers.

    Techniques Used:

    na ca exchanger 1  (Alomone Labs)


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    anti ncx1  (Alomone Labs)


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    Alomone Labs anti ncx1
    Effect of NCX3 silencing or inhibition by KB-R7943 in WT and Tg2576 primary hippocampal neurons. (A) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded in WT and WT plus siNCX3 (black traces), Tg2576 and Tg2576 plus siNCX3 (grey traces) primary hippocampal neurons at 12 DIV. (B) Quantification of I NCX in the reverse mode of operation represented in A, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. (C) Representative Western blotting experiments and relative quantifications showing the effect of NCX3 silencing (siNCX3) on NCX3, <t>NCX1,</t> and NCX2 protein expression in primary hippocampal neurons (D) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded from WT and WT plus 0.5 μM kB-R7943 (black traces), Tg2576 and Tg2576 plus 0.5 μM kB-R7943 (grey traces) primary hippocampal neurons at 12 DIV. (E) Quantification of I NCX in the reverse mode of operation represented in D, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. The number of cells used for each experimental condition is noted on the bars. (F, G) Representative Western blot of NCX3 protein expression and densitometric quantification of NCX3 truncated band in WT and Tg2576 primary hippocampal neurons at 12 DIV, represented as percentage of WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. Statistical comparisons between groups were performed by one-way ANOVA followed by Newman-Keuls’ test. (* p < 0.05 vs. WT; ** p < 0.05 vs. Tg2576 mice).
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    1) Product Images from "The Na + /Ca 2+ Exchanger 3 Is Functionally Coupled With the Na V 1.6 Voltage-Gated Channel and Promotes an Endoplasmic Reticulum Ca 2+ Refilling in a Transgenic Model of Alzheimer’s Disease"

    Article Title: The Na + /Ca 2+ Exchanger 3 Is Functionally Coupled With the Na V 1.6 Voltage-Gated Channel and Promotes an Endoplasmic Reticulum Ca 2+ Refilling in a Transgenic Model of Alzheimer’s Disease

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.775271

    Effect of NCX3 silencing or inhibition by KB-R7943 in WT and Tg2576 primary hippocampal neurons. (A) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded in WT and WT plus siNCX3 (black traces), Tg2576 and Tg2576 plus siNCX3 (grey traces) primary hippocampal neurons at 12 DIV. (B) Quantification of I NCX in the reverse mode of operation represented in A, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. (C) Representative Western blotting experiments and relative quantifications showing the effect of NCX3 silencing (siNCX3) on NCX3, NCX1, and NCX2 protein expression in primary hippocampal neurons (D) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded from WT and WT plus 0.5 μM kB-R7943 (black traces), Tg2576 and Tg2576 plus 0.5 μM kB-R7943 (grey traces) primary hippocampal neurons at 12 DIV. (E) Quantification of I NCX in the reverse mode of operation represented in D, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. The number of cells used for each experimental condition is noted on the bars. (F, G) Representative Western blot of NCX3 protein expression and densitometric quantification of NCX3 truncated band in WT and Tg2576 primary hippocampal neurons at 12 DIV, represented as percentage of WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. Statistical comparisons between groups were performed by one-way ANOVA followed by Newman-Keuls’ test. (* p < 0.05 vs. WT; ** p < 0.05 vs. Tg2576 mice).
    Figure Legend Snippet: Effect of NCX3 silencing or inhibition by KB-R7943 in WT and Tg2576 primary hippocampal neurons. (A) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded in WT and WT plus siNCX3 (black traces), Tg2576 and Tg2576 plus siNCX3 (grey traces) primary hippocampal neurons at 12 DIV. (B) Quantification of I NCX in the reverse mode of operation represented in A, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. (C) Representative Western blotting experiments and relative quantifications showing the effect of NCX3 silencing (siNCX3) on NCX3, NCX1, and NCX2 protein expression in primary hippocampal neurons (D) Representative superimposed traces of I NCX in the reverse and forward modes of operation recorded from WT and WT plus 0.5 μM kB-R7943 (black traces), Tg2576 and Tg2576 plus 0.5 μM kB-R7943 (grey traces) primary hippocampal neurons at 12 DIV. (E) Quantification of I NCX in the reverse mode of operation represented in D, expressed as percentage of variation in comparison to WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. The number of cells used for each experimental condition is noted on the bars. (F, G) Representative Western blot of NCX3 protein expression and densitometric quantification of NCX3 truncated band in WT and Tg2576 primary hippocampal neurons at 12 DIV, represented as percentage of WT. Values are expressed as mean ± SEM of 3 independent experimental sessions. Statistical comparisons between groups were performed by one-way ANOVA followed by Newman-Keuls’ test. (* p < 0.05 vs. WT; ** p < 0.05 vs. Tg2576 mice).

    Techniques Used: Inhibition, Western Blot, Expressing

    anti ncx1  (Alomone Labs)


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    Gene and protein expression ( A – C ). Expression levels of CACNA1C , SLC8A1 and the connexin members, GJA5 , GJA1 and GJC1 in HL-1 cells transfected with miR-NC (black columns) or miR-199a+miR-22 (white columns). Normalized to miR-NC ( D – F ). Protein expression levels of Cav1.2, <t>NCX1</t> and Cx40, normalized to the endogenous control, tubulin ( G ). Representative WB experiments in HL-1 cells transfected with miR-22+199a and miR-NC. All proteins (Cav1.2, NCX1, connexin 40 and tubulin) were blotted on the same gel. This is a representative image of four independent experiments. Numbers within columns represent the number of independent experiments done. * p < 0.05, *** p < 0.0001, n.s.: non-significant.
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    1) Product Images from "Atrial Fibrillation in Heart Failure Is Associated with High Levels of Circulating microRNA-199a-5p and 22–5p and a Defective Regulation of Intracellular Calcium and Cell-to-Cell Communication"

    Article Title: Atrial Fibrillation in Heart Failure Is Associated with High Levels of Circulating microRNA-199a-5p and 22–5p and a Defective Regulation of Intracellular Calcium and Cell-to-Cell Communication

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms221910377

    Gene and protein expression ( A – C ). Expression levels of CACNA1C , SLC8A1 and the connexin members, GJA5 , GJA1 and GJC1 in HL-1 cells transfected with miR-NC (black columns) or miR-199a+miR-22 (white columns). Normalized to miR-NC ( D – F ). Protein expression levels of Cav1.2, NCX1 and Cx40, normalized to the endogenous control, tubulin ( G ). Representative WB experiments in HL-1 cells transfected with miR-22+199a and miR-NC. All proteins (Cav1.2, NCX1, connexin 40 and tubulin) were blotted on the same gel. This is a representative image of four independent experiments. Numbers within columns represent the number of independent experiments done. * p < 0.05, *** p < 0.0001, n.s.: non-significant.
    Figure Legend Snippet: Gene and protein expression ( A – C ). Expression levels of CACNA1C , SLC8A1 and the connexin members, GJA5 , GJA1 and GJC1 in HL-1 cells transfected with miR-NC (black columns) or miR-199a+miR-22 (white columns). Normalized to miR-NC ( D – F ). Protein expression levels of Cav1.2, NCX1 and Cx40, normalized to the endogenous control, tubulin ( G ). Representative WB experiments in HL-1 cells transfected with miR-22+199a and miR-NC. All proteins (Cav1.2, NCX1, connexin 40 and tubulin) were blotted on the same gel. This is a representative image of four independent experiments. Numbers within columns represent the number of independent experiments done. * p < 0.05, *** p < 0.0001, n.s.: non-significant.

    Techniques Used: Expressing, Transfection

    anti ncx  (Alomone Labs)


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    ncx  (Alomone Labs)


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    anx 011  (Alomone Labs)


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    Alomone Labs anx 011
    Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 <t>(Anx-011,</t> Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.
    Anx 011, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Ionotropic P2X4 Receptor has Unique Properties in the Heart by Mediating the Negative Chronotropic Effect of ATP While Increasing the Ventricular Inotropy"

    Article Title: The Ionotropic P2X4 Receptor has Unique Properties in the Heart by Mediating the Negative Chronotropic Effect of ATP While Increasing the Ventricular Inotropy

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2019.01103

    Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.
    Figure Legend Snippet: Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.

    Techniques Used: Expressing, Immunostaining

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    Alomone Labs anti ncx1
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    Alomone Labs anti ncx
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    Alomone Labs anx 011
    Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 <t>(Anx-011,</t> Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.
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    Image Search Results


    Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.

    Journal: Frontiers in Pharmacology

    Article Title: The Ionotropic P2X4 Receptor has Unique Properties in the Heart by Mediating the Negative Chronotropic Effect of ATP While Increasing the Ventricular Inotropy

    doi: 10.3389/fphar.2019.01103

    Figure Lengend Snippet: Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.

    Article Snippet: NCX1 , Anx-011 , Rabbit (rb) , 1:50 , Alomone.

    Techniques: Expressing, Immunostaining