nhe1  (Alomone Labs)


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    Name:
    Anti Na H Exchanger 1 NHE 1 extracellular Antibody
    Description:
    Anti Na H Exchanger 1 NHE 1 Antibody ANX 010 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunohistochemistry immunocytochemistry live cell imaging and indirect live cell flow cytometry applications It has been designed to recognize NHE 1 from human rat and mouse samples
    Catalog Number:
    ANX-010
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Immunohistochemistry, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs nhe1
    Anti Na H Exchanger 1 NHE 1 extracellular Antibody
    Anti Na H Exchanger 1 NHE 1 Antibody ANX 010 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunohistochemistry immunocytochemistry live cell imaging and indirect live cell flow cytometry applications It has been designed to recognize NHE 1 from human rat and mouse samples
    https://www.bioz.com/result/nhe1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nhe1 - by Bioz Stars, 2021-09
    92/100 stars

    Images

    1) Product Images from "Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells"

    Article Title: Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-07756-x

    Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα ( a ), ENaCδ ( b ), and NHE1 ( c ) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca 2+ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. * p
    Figure Legend Snippet: Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα ( a ), ENaCδ ( b ), and NHE1 ( c ) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca 2+ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. * p

    Techniques Used: Transfection, Concentration Assay

    ENaCα, ENaCδ, and NHE1 mRNA expression in both transfected and untransfected cells. mRNA expressions of ENaCα (a) , ENaCδ (b) , and NHE1 (c) were significantly decreased in siRNA-transfected cells. Each experiment was performed three times. * p
    Figure Legend Snippet: ENaCα, ENaCδ, and NHE1 mRNA expression in both transfected and untransfected cells. mRNA expressions of ENaCα (a) , ENaCδ (b) , and NHE1 (c) were significantly decreased in siRNA-transfected cells. Each experiment was performed three times. * p

    Techniques Used: Expressing, Transfection

    ENaCα, ENaCδ, and NHE1 protein expression in transfected and untransfected cells. ENaCα, ENaCδ, and NHE1 are visualized with a red color; nuclei are stained with a blue color. (a) HBO cells express ENaCα proteins. (b) Percent cells expressing ENaCα in untransfected cells (30.2%, 88 of 291 cells), ENaCα siRNA transfected cells (1.5%, 5 of 327 cells), and scrambled siRNA- transfected cells (27.9%, 69 of 247 cells). (c) The ENaCδ proteins express in HBO cells. (d) Percent cells expressing ENaCδ in untransfected cells (27.8%, 49 of 176 cells), ENaCδ siRNA transfected cells (2.6%, 6 of 232 cells), and scrambled siRNA-transfected cells (22.5%, 29 of 129 cells). (e) Immunoreactivity of NHE1 antibodies demonstrates the presence of NHE1 expression in HBO cells. (f) Percent cells expressing NHE1 in untransfected cells (33.0%, 69 of 209 cells), NHE1 siRNA-transfected cells (1.8%, 5 of 280 cells), and scrambled siRNA-transfected cells (30.6%, 19 of 62 cells). Each experiment was performed three times. * p
    Figure Legend Snippet: ENaCα, ENaCδ, and NHE1 protein expression in transfected and untransfected cells. ENaCα, ENaCδ, and NHE1 are visualized with a red color; nuclei are stained with a blue color. (a) HBO cells express ENaCα proteins. (b) Percent cells expressing ENaCα in untransfected cells (30.2%, 88 of 291 cells), ENaCα siRNA transfected cells (1.5%, 5 of 327 cells), and scrambled siRNA- transfected cells (27.9%, 69 of 247 cells). (c) The ENaCδ proteins express in HBO cells. (d) Percent cells expressing ENaCδ in untransfected cells (27.8%, 49 of 176 cells), ENaCδ siRNA transfected cells (2.6%, 6 of 232 cells), and scrambled siRNA-transfected cells (22.5%, 29 of 129 cells). (e) Immunoreactivity of NHE1 antibodies demonstrates the presence of NHE1 expression in HBO cells. (f) Percent cells expressing NHE1 in untransfected cells (33.0%, 69 of 209 cells), NHE1 siRNA-transfected cells (1.8%, 5 of 280 cells), and scrambled siRNA-transfected cells (30.6%, 19 of 62 cells). Each experiment was performed three times. * p

    Techniques Used: Expressing, Transfection, Staining

    Related Articles

    Staining:

    Article Title: Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde for 15 min at room temperature and then permeabilized with 10% methanol and 10% hydrogen peroxide in PBS for 15 min. Then cells were blocked with 3% goat serum, 3% bovine serum albumin in PBS for 1 h and stained with primary antibodies ENaCα and ENaCδ (LSBio) and NHE1 (Alomone Labs) overnight at 4 °C, followed by goat anti-rabbit secondary antibody (IgG Alexa 635, 1:500) for 30 min at room temperature. ..

    Incubation:

    Article Title: Na+/H+ exchanger isoform 1 activity in AQP2-expressing cells can be either proliferative or anti-proliferative depending on extracellular pH.
    Article Snippet: .. We have previously shown in renal cells that expression of the water channel Aquaporin-2 increases cell proliferation by a regulatory volume mechanism involving Na < sup > + < /sup > /H < sup > + < /sup > exchanger isoform 2.. Here, we investigated if Aquaporin-2 (AQP2) also modulates Na < sup > + < /sup > /H < sup > + < /sup > exchanger isoform 1-dependent cell proliferation. ..

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  • 92
    Alomone Labs nhe1
    Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα ( a ), ENaCδ ( b ), and <t>NHE1</t> ( c ) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca 2+ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. * p
    Nhe1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhe1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nhe1 - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

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    Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα ( a ), ENaCδ ( b ), and NHE1 ( c ) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca 2+ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. * p

    Journal: Scientific Reports

    Article Title: Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells

    doi: 10.1038/s41598-017-07756-x

    Figure Lengend Snippet: Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα ( a ), ENaCδ ( b ), and NHE1 ( c ) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca 2+ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. * p

    Article Snippet: The cells were fixed with 4% paraformaldehyde for 15 min at room temperature and then permeabilized with 10% methanol and 10% hydrogen peroxide in PBS for 15 min. Then cells were blocked with 3% goat serum, 3% bovine serum albumin in PBS for 1 h and stained with primary antibodies ENaCα and ENaCδ (LSBio) and NHE1 (Alomone Labs) overnight at 4 °C, followed by goat anti-rabbit secondary antibody (IgG Alexa 635, 1:500) for 30 min at room temperature.

    Techniques: Transfection, Concentration Assay

    ENaCα, ENaCδ, and NHE1 mRNA expression in both transfected and untransfected cells. mRNA expressions of ENaCα (a) , ENaCδ (b) , and NHE1 (c) were significantly decreased in siRNA-transfected cells. Each experiment was performed three times. * p

    Journal: Scientific Reports

    Article Title: Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells

    doi: 10.1038/s41598-017-07756-x

    Figure Lengend Snippet: ENaCα, ENaCδ, and NHE1 mRNA expression in both transfected and untransfected cells. mRNA expressions of ENaCα (a) , ENaCδ (b) , and NHE1 (c) were significantly decreased in siRNA-transfected cells. Each experiment was performed three times. * p

    Article Snippet: The cells were fixed with 4% paraformaldehyde for 15 min at room temperature and then permeabilized with 10% methanol and 10% hydrogen peroxide in PBS for 15 min. Then cells were blocked with 3% goat serum, 3% bovine serum albumin in PBS for 1 h and stained with primary antibodies ENaCα and ENaCδ (LSBio) and NHE1 (Alomone Labs) overnight at 4 °C, followed by goat anti-rabbit secondary antibody (IgG Alexa 635, 1:500) for 30 min at room temperature.

    Techniques: Expressing, Transfection

    ENaCα, ENaCδ, and NHE1 protein expression in transfected and untransfected cells. ENaCα, ENaCδ, and NHE1 are visualized with a red color; nuclei are stained with a blue color. (a) HBO cells express ENaCα proteins. (b) Percent cells expressing ENaCα in untransfected cells (30.2%, 88 of 291 cells), ENaCα siRNA transfected cells (1.5%, 5 of 327 cells), and scrambled siRNA- transfected cells (27.9%, 69 of 247 cells). (c) The ENaCδ proteins express in HBO cells. (d) Percent cells expressing ENaCδ in untransfected cells (27.8%, 49 of 176 cells), ENaCδ siRNA transfected cells (2.6%, 6 of 232 cells), and scrambled siRNA-transfected cells (22.5%, 29 of 129 cells). (e) Immunoreactivity of NHE1 antibodies demonstrates the presence of NHE1 expression in HBO cells. (f) Percent cells expressing NHE1 in untransfected cells (33.0%, 69 of 209 cells), NHE1 siRNA-transfected cells (1.8%, 5 of 280 cells), and scrambled siRNA-transfected cells (30.6%, 19 of 62 cells). Each experiment was performed three times. * p

    Journal: Scientific Reports

    Article Title: Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells

    doi: 10.1038/s41598-017-07756-x

    Figure Lengend Snippet: ENaCα, ENaCδ, and NHE1 protein expression in transfected and untransfected cells. ENaCα, ENaCδ, and NHE1 are visualized with a red color; nuclei are stained with a blue color. (a) HBO cells express ENaCα proteins. (b) Percent cells expressing ENaCα in untransfected cells (30.2%, 88 of 291 cells), ENaCα siRNA transfected cells (1.5%, 5 of 327 cells), and scrambled siRNA- transfected cells (27.9%, 69 of 247 cells). (c) The ENaCδ proteins express in HBO cells. (d) Percent cells expressing ENaCδ in untransfected cells (27.8%, 49 of 176 cells), ENaCδ siRNA transfected cells (2.6%, 6 of 232 cells), and scrambled siRNA-transfected cells (22.5%, 29 of 129 cells). (e) Immunoreactivity of NHE1 antibodies demonstrates the presence of NHE1 expression in HBO cells. (f) Percent cells expressing NHE1 in untransfected cells (33.0%, 69 of 209 cells), NHE1 siRNA-transfected cells (1.8%, 5 of 280 cells), and scrambled siRNA-transfected cells (30.6%, 19 of 62 cells). Each experiment was performed three times. * p

    Article Snippet: The cells were fixed with 4% paraformaldehyde for 15 min at room temperature and then permeabilized with 10% methanol and 10% hydrogen peroxide in PBS for 15 min. Then cells were blocked with 3% goat serum, 3% bovine serum albumin in PBS for 1 h and stained with primary antibodies ENaCα and ENaCδ (LSBio) and NHE1 (Alomone Labs) overnight at 4 °C, followed by goat anti-rabbit secondary antibody (IgG Alexa 635, 1:500) for 30 min at room temperature.

    Techniques: Expressing, Transfection, Staining