nhe1  (Alomone Labs)


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    Alomone Labs nhe1
    Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα ( a ), ENaCδ ( b ), and <t>NHE1</t> ( c ) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca 2+ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. * p
    Nhe1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhe1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nhe1 - by Bioz Stars, 2022-06
    93/100 stars

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    1) Product Images from "Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells"

    Article Title: Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-07756-x

    Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα ( a ), ENaCδ ( b ), and NHE1 ( c ) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca 2+ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. * p
    Figure Legend Snippet: Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα ( a ), ENaCδ ( b ), and NHE1 ( c ) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca 2+ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. * p

    Techniques Used: Transfection, Concentration Assay

    ENaCα, ENaCδ, and NHE1 mRNA expression in both transfected and untransfected cells. mRNA expressions of ENaCα (a) , ENaCδ (b) , and NHE1 (c) were significantly decreased in siRNA-transfected cells. Each experiment was performed three times. * p
    Figure Legend Snippet: ENaCα, ENaCδ, and NHE1 mRNA expression in both transfected and untransfected cells. mRNA expressions of ENaCα (a) , ENaCδ (b) , and NHE1 (c) were significantly decreased in siRNA-transfected cells. Each experiment was performed three times. * p

    Techniques Used: Expressing, Transfection

    ENaCα, ENaCδ, and NHE1 protein expression in transfected and untransfected cells. ENaCα, ENaCδ, and NHE1 are visualized with a red color; nuclei are stained with a blue color. (a) HBO cells express ENaCα proteins. (b) Percent cells expressing ENaCα in untransfected cells (30.2%, 88 of 291 cells), ENaCα siRNA transfected cells (1.5%, 5 of 327 cells), and scrambled siRNA- transfected cells (27.9%, 69 of 247 cells). (c) The ENaCδ proteins express in HBO cells. (d) Percent cells expressing ENaCδ in untransfected cells (27.8%, 49 of 176 cells), ENaCδ siRNA transfected cells (2.6%, 6 of 232 cells), and scrambled siRNA-transfected cells (22.5%, 29 of 129 cells). (e) Immunoreactivity of NHE1 antibodies demonstrates the presence of NHE1 expression in HBO cells. (f) Percent cells expressing NHE1 in untransfected cells (33.0%, 69 of 209 cells), NHE1 siRNA-transfected cells (1.8%, 5 of 280 cells), and scrambled siRNA-transfected cells (30.6%, 19 of 62 cells). Each experiment was performed three times. * p
    Figure Legend Snippet: ENaCα, ENaCδ, and NHE1 protein expression in transfected and untransfected cells. ENaCα, ENaCδ, and NHE1 are visualized with a red color; nuclei are stained with a blue color. (a) HBO cells express ENaCα proteins. (b) Percent cells expressing ENaCα in untransfected cells (30.2%, 88 of 291 cells), ENaCα siRNA transfected cells (1.5%, 5 of 327 cells), and scrambled siRNA- transfected cells (27.9%, 69 of 247 cells). (c) The ENaCδ proteins express in HBO cells. (d) Percent cells expressing ENaCδ in untransfected cells (27.8%, 49 of 176 cells), ENaCδ siRNA transfected cells (2.6%, 6 of 232 cells), and scrambled siRNA-transfected cells (22.5%, 29 of 129 cells). (e) Immunoreactivity of NHE1 antibodies demonstrates the presence of NHE1 expression in HBO cells. (f) Percent cells expressing NHE1 in untransfected cells (33.0%, 69 of 209 cells), NHE1 siRNA-transfected cells (1.8%, 5 of 280 cells), and scrambled siRNA-transfected cells (30.6%, 19 of 62 cells). Each experiment was performed three times. * p

    Techniques Used: Expressing, Transfection, Staining

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    Alomone Labs anti na h exchanger 1 nhe 1 extracellular antibody
    Effects of cigarette smoke extract (CSE) treatment and smoking on the activity of Na + /H + <t>exchanger-1</t> (NHE-1) in guinea pig esophageal epithelial cells (EECs). ( a ) Normal guinea pig EECs were pretreated with different concentrations of CSE (1, 10 and 100 µg/mL) for 1 h and the activity of NHE-1 was measured. Representative intracellular pH (pH i ) curves show the recovery from acidosis. ( b ) Summary data of the calculated activity of NHE-1 in guinea pig EECs. The rate of pH recovery ( J (B − )) was calculated as described in Figure 2 b. Data are presented as the mean ± SEM; a: p ≤ 0.05 vs. control; n = 13–18 exp./86–99 ROIs. ( c ) Chronic effect of cigarette smoking was investigated, using smoking chamber. Guinea pigs were exposed to whole body cigarette smoke 4 times a day, 5 days a week, 30 min each time, using a TE2 closed-chamber manual smoking system. After 1, 2, or 4 months of smoking, the animals were sacrificed and NHE-1 activity was measured. ( d ) Summary data of the calculated activity of NHE-1 in guinea pig EECs. The rate of pH recovery ( J (B − )) was calculated as described in Figure 2 b. Data are presented as the mean ± SEM; a: p ≤ 0.05 vs. control; n = 8–16exp./81–210 ROIs.
    Anti Na H Exchanger 1 Nhe 1 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of cigarette smoke extract (CSE) treatment and smoking on the activity of Na + /H + exchanger-1 (NHE-1) in guinea pig esophageal epithelial cells (EECs). ( a ) Normal guinea pig EECs were pretreated with different concentrations of CSE (1, 10 and 100 µg/mL) for 1 h and the activity of NHE-1 was measured. Representative intracellular pH (pH i ) curves show the recovery from acidosis. ( b ) Summary data of the calculated activity of NHE-1 in guinea pig EECs. The rate of pH recovery ( J (B − )) was calculated as described in Figure 2 b. Data are presented as the mean ± SEM; a: p ≤ 0.05 vs. control; n = 13–18 exp./86–99 ROIs. ( c ) Chronic effect of cigarette smoking was investigated, using smoking chamber. Guinea pigs were exposed to whole body cigarette smoke 4 times a day, 5 days a week, 30 min each time, using a TE2 closed-chamber manual smoking system. After 1, 2, or 4 months of smoking, the animals were sacrificed and NHE-1 activity was measured. ( d ) Summary data of the calculated activity of NHE-1 in guinea pig EECs. The rate of pH recovery ( J (B − )) was calculated as described in Figure 2 b. Data are presented as the mean ± SEM; a: p ≤ 0.05 vs. control; n = 8–16exp./81–210 ROIs.

    Journal: International Journal of Molecular Sciences

    Article Title: Inhibition of NHE-1 Increases Smoke-Induced Proliferative Activity of Barrett’s Esophageal Cell Line

    doi: 10.3390/ijms221910581

    Figure Lengend Snippet: Effects of cigarette smoke extract (CSE) treatment and smoking on the activity of Na + /H + exchanger-1 (NHE-1) in guinea pig esophageal epithelial cells (EECs). ( a ) Normal guinea pig EECs were pretreated with different concentrations of CSE (1, 10 and 100 µg/mL) for 1 h and the activity of NHE-1 was measured. Representative intracellular pH (pH i ) curves show the recovery from acidosis. ( b ) Summary data of the calculated activity of NHE-1 in guinea pig EECs. The rate of pH recovery ( J (B − )) was calculated as described in Figure 2 b. Data are presented as the mean ± SEM; a: p ≤ 0.05 vs. control; n = 13–18 exp./86–99 ROIs. ( c ) Chronic effect of cigarette smoking was investigated, using smoking chamber. Guinea pigs were exposed to whole body cigarette smoke 4 times a day, 5 days a week, 30 min each time, using a TE2 closed-chamber manual smoking system. After 1, 2, or 4 months of smoking, the animals were sacrificed and NHE-1 activity was measured. ( d ) Summary data of the calculated activity of NHE-1 in guinea pig EECs. The rate of pH recovery ( J (B − )) was calculated as described in Figure 2 b. Data are presented as the mean ± SEM; a: p ≤ 0.05 vs. control; n = 8–16exp./81–210 ROIs.

    Article Snippet: Membranes were incubated overnight with rabbit polyclonal anti-NHE-1 (Catalogue No. ANX-010, Alomone Labs, Jerusalem, Israel), mouse monoclonal anti-GAPDH (Catalogue No. MAB 374, Sigma Aldrich, Hungary) or mouse monoclonal anti-α-Tubulin antibody (Catalogue No. T9026, Merck, Darmstadt, Germany) followed by the incubation with the appropriate HRP-conjugated secondary antibody (Catalogue No. P0448 goat anti-rabbit and P0161 rabbit anti-mouse, DAKO, Glostrup, Denmark or G-21040 goat anti-mouse, Invitrogen, Watham, MA, USA).

    Techniques: Activity Assay

    Knockdown of Na + /H + exchanger-1 (NHE-1) in human esophageal cell lines. The expression levels of NHE-1 were investigated by RT-PCR ( a ) and immunohistochemistry ( b ) in control cells and in cells treated with specific siRNA for SLC9A1 . The rate of proliferation was determined in the non-treated ( c ) and cigarette smoke extract-treated ( d ) CP-A and CP-D cells. Data represent mean ± SEM of three independent experiments; a = p ≤ 0.05 vs. control.

    Journal: International Journal of Molecular Sciences

    Article Title: Inhibition of NHE-1 Increases Smoke-Induced Proliferative Activity of Barrett’s Esophageal Cell Line

    doi: 10.3390/ijms221910581

    Figure Lengend Snippet: Knockdown of Na + /H + exchanger-1 (NHE-1) in human esophageal cell lines. The expression levels of NHE-1 were investigated by RT-PCR ( a ) and immunohistochemistry ( b ) in control cells and in cells treated with specific siRNA for SLC9A1 . The rate of proliferation was determined in the non-treated ( c ) and cigarette smoke extract-treated ( d ) CP-A and CP-D cells. Data represent mean ± SEM of three independent experiments; a = p ≤ 0.05 vs. control.

    Article Snippet: Membranes were incubated overnight with rabbit polyclonal anti-NHE-1 (Catalogue No. ANX-010, Alomone Labs, Jerusalem, Israel), mouse monoclonal anti-GAPDH (Catalogue No. MAB 374, Sigma Aldrich, Hungary) or mouse monoclonal anti-α-Tubulin antibody (Catalogue No. T9026, Merck, Darmstadt, Germany) followed by the incubation with the appropriate HRP-conjugated secondary antibody (Catalogue No. P0448 goat anti-rabbit and P0161 rabbit anti-mouse, DAKO, Glostrup, Denmark or G-21040 goat anti-mouse, Invitrogen, Watham, MA, USA).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry

    Effects of cigarette smoke extract (CSE) treatment on the mRNA and protein expression of Na + /H + exchanger-1 (NHE-1) in esophageal cell lines. Metaplastic (CP-A) and dysplastic (CP-D) esophageal cell lines were treated with dif-ferent concentrations of CSE (1 and 10 µg/mL) for 6, 24 and 72 h, and the relative gene ( a ) and protein ( b ) expressions of NHE-1 were investigated by real-time PCR and Western blot, respectively. GAPDH was used as a protein-loading control. Data represent mean ± SEM of three independent experiments; a = p ≤ 0.05 vs. control.

    Journal: International Journal of Molecular Sciences

    Article Title: Inhibition of NHE-1 Increases Smoke-Induced Proliferative Activity of Barrett’s Esophageal Cell Line

    doi: 10.3390/ijms221910581

    Figure Lengend Snippet: Effects of cigarette smoke extract (CSE) treatment on the mRNA and protein expression of Na + /H + exchanger-1 (NHE-1) in esophageal cell lines. Metaplastic (CP-A) and dysplastic (CP-D) esophageal cell lines were treated with dif-ferent concentrations of CSE (1 and 10 µg/mL) for 6, 24 and 72 h, and the relative gene ( a ) and protein ( b ) expressions of NHE-1 were investigated by real-time PCR and Western blot, respectively. GAPDH was used as a protein-loading control. Data represent mean ± SEM of three independent experiments; a = p ≤ 0.05 vs. control.

    Article Snippet: Membranes were incubated overnight with rabbit polyclonal anti-NHE-1 (Catalogue No. ANX-010, Alomone Labs, Jerusalem, Israel), mouse monoclonal anti-GAPDH (Catalogue No. MAB 374, Sigma Aldrich, Hungary) or mouse monoclonal anti-α-Tubulin antibody (Catalogue No. T9026, Merck, Darmstadt, Germany) followed by the incubation with the appropriate HRP-conjugated secondary antibody (Catalogue No. P0448 goat anti-rabbit and P0161 rabbit anti-mouse, DAKO, Glostrup, Denmark or G-21040 goat anti-mouse, Invitrogen, Watham, MA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    Effects of cigarette smoke extract (CSE) treatment on the activity of Na + /H + exchanger-1 (NHE-1) in esophageal cell lines. Metaplastic (CP-A) and dysplastic (CP-D) esophageal cell lines were pretreated with different concentrations of CSE (1, 10 and 100 µg/mL) for 1 h, and the activity of NHE-1 was measured. ( a ) Representative intracellular pH (pH i ) curves present the recovery from acidosis in CP-A cells. ( b ) Summary data of the calculated activity of NHE-1 in the different cell lines. The rate of pH recovery ( J (B − )) was calculated as described in Figure 2 b. Data are presented as the mean ± SEM; a = p ≤ 0.05 vs. control; n = 12–14 exp./66–68 ROIs.

    Journal: International Journal of Molecular Sciences

    Article Title: Inhibition of NHE-1 Increases Smoke-Induced Proliferative Activity of Barrett’s Esophageal Cell Line

    doi: 10.3390/ijms221910581

    Figure Lengend Snippet: Effects of cigarette smoke extract (CSE) treatment on the activity of Na + /H + exchanger-1 (NHE-1) in esophageal cell lines. Metaplastic (CP-A) and dysplastic (CP-D) esophageal cell lines were pretreated with different concentrations of CSE (1, 10 and 100 µg/mL) for 1 h, and the activity of NHE-1 was measured. ( a ) Representative intracellular pH (pH i ) curves present the recovery from acidosis in CP-A cells. ( b ) Summary data of the calculated activity of NHE-1 in the different cell lines. The rate of pH recovery ( J (B − )) was calculated as described in Figure 2 b. Data are presented as the mean ± SEM; a = p ≤ 0.05 vs. control; n = 12–14 exp./66–68 ROIs.

    Article Snippet: Membranes were incubated overnight with rabbit polyclonal anti-NHE-1 (Catalogue No. ANX-010, Alomone Labs, Jerusalem, Israel), mouse monoclonal anti-GAPDH (Catalogue No. MAB 374, Sigma Aldrich, Hungary) or mouse monoclonal anti-α-Tubulin antibody (Catalogue No. T9026, Merck, Darmstadt, Germany) followed by the incubation with the appropriate HRP-conjugated secondary antibody (Catalogue No. P0448 goat anti-rabbit and P0161 rabbit anti-mouse, DAKO, Glostrup, Denmark or G-21040 goat anti-mouse, Invitrogen, Watham, MA, USA).

    Techniques: Activity Assay

    Activity, mRNA and protein expression of Na + /H + exchanger-1 (NHE-1) in esophageal cell lines. ( a ) Representative intracellular pH (pH i ) curves present the recovery from acidosis in CP-A and CP-D cells. ( b ) Summary data of the calculated activity of NHE-1 in the different cell lines. The rate of pH recovery ( J (B − )) was calculated from the ΔpH/Δt obtained via linear regression analysis of the pH i measurement performed over the first 60 s of recovery from the lowest pH i level (initial pH i ). The buffering capacity at the initial pH i was used to calculate J (B − ). Data are presented as the mean ± SEM. a: p ≤ 0.05 vs. CP-A; n = 5–11 exp./26–91 region of interests (ROIs). ( c ) mRNA and ( d ) protein expression of NHE-1 in the CP-A and CP-D cells. α-Tubulin was used as a protein-loading control. Data represent mean ± SEM of three independent experiments.

    Journal: International Journal of Molecular Sciences

    Article Title: Inhibition of NHE-1 Increases Smoke-Induced Proliferative Activity of Barrett’s Esophageal Cell Line

    doi: 10.3390/ijms221910581

    Figure Lengend Snippet: Activity, mRNA and protein expression of Na + /H + exchanger-1 (NHE-1) in esophageal cell lines. ( a ) Representative intracellular pH (pH i ) curves present the recovery from acidosis in CP-A and CP-D cells. ( b ) Summary data of the calculated activity of NHE-1 in the different cell lines. The rate of pH recovery ( J (B − )) was calculated from the ΔpH/Δt obtained via linear regression analysis of the pH i measurement performed over the first 60 s of recovery from the lowest pH i level (initial pH i ). The buffering capacity at the initial pH i was used to calculate J (B − ). Data are presented as the mean ± SEM. a: p ≤ 0.05 vs. CP-A; n = 5–11 exp./26–91 region of interests (ROIs). ( c ) mRNA and ( d ) protein expression of NHE-1 in the CP-A and CP-D cells. α-Tubulin was used as a protein-loading control. Data represent mean ± SEM of three independent experiments.

    Article Snippet: Membranes were incubated overnight with rabbit polyclonal anti-NHE-1 (Catalogue No. ANX-010, Alomone Labs, Jerusalem, Israel), mouse monoclonal anti-GAPDH (Catalogue No. MAB 374, Sigma Aldrich, Hungary) or mouse monoclonal anti-α-Tubulin antibody (Catalogue No. T9026, Merck, Darmstadt, Germany) followed by the incubation with the appropriate HRP-conjugated secondary antibody (Catalogue No. P0448 goat anti-rabbit and P0161 rabbit anti-mouse, DAKO, Glostrup, Denmark or G-21040 goat anti-mouse, Invitrogen, Watham, MA, USA).

    Techniques: Activity Assay, Expressing

    Expression of Na + /H + exchanger-1 (NHE-1) in human esophageal samples. ( a ) Representative immunohistoche-mical stainings show the presence of NHE-1 in human esophageal samples. Scale bar represents 100 µm. ( b ) Quantification of DAB intensities were calculated, using a semi-quantitative scoring system. Data represent mean ± SEM of 23–25 specimens/3–6 patients each group; a = p ≤ 0.05 vs. normal; b = p ≤ 0.05 vs. non-smoker. BE: Barrett’s esophagus.

    Journal: International Journal of Molecular Sciences

    Article Title: Inhibition of NHE-1 Increases Smoke-Induced Proliferative Activity of Barrett’s Esophageal Cell Line

    doi: 10.3390/ijms221910581

    Figure Lengend Snippet: Expression of Na + /H + exchanger-1 (NHE-1) in human esophageal samples. ( a ) Representative immunohistoche-mical stainings show the presence of NHE-1 in human esophageal samples. Scale bar represents 100 µm. ( b ) Quantification of DAB intensities were calculated, using a semi-quantitative scoring system. Data represent mean ± SEM of 23–25 specimens/3–6 patients each group; a = p ≤ 0.05 vs. normal; b = p ≤ 0.05 vs. non-smoker. BE: Barrett’s esophagus.

    Article Snippet: Membranes were incubated overnight with rabbit polyclonal anti-NHE-1 (Catalogue No. ANX-010, Alomone Labs, Jerusalem, Israel), mouse monoclonal anti-GAPDH (Catalogue No. MAB 374, Sigma Aldrich, Hungary) or mouse monoclonal anti-α-Tubulin antibody (Catalogue No. T9026, Merck, Darmstadt, Germany) followed by the incubation with the appropriate HRP-conjugated secondary antibody (Catalogue No. P0448 goat anti-rabbit and P0161 rabbit anti-mouse, DAKO, Glostrup, Denmark or G-21040 goat anti-mouse, Invitrogen, Watham, MA, USA).

    Techniques: Expressing

    Representative immunoblotting for NHE1 ( A ), incubation of anti-NHE1 with the blocking peptide ( C ), and total protein content ( B , D ). Lanes: (MW) protein ladder; (1–6) sperm samples, each one corresponding to a different male; (7) pig ovary tissue sample; (8) pig oviduct tissue sample; (9) pig epididymis tissue sample.

    Journal: International Journal of Molecular Sciences

    Article Title: Blocking NHE Channels Reduces the Ability of In Vitro Capacitated Mammalian Sperm to Respond to Progesterone Stimulus

    doi: 10.3390/ijms222312646

    Figure Lengend Snippet: Representative immunoblotting for NHE1 ( A ), incubation of anti-NHE1 with the blocking peptide ( C ), and total protein content ( B , D ). Lanes: (MW) protein ladder; (1–6) sperm samples, each one corresponding to a different male; (7) pig ovary tissue sample; (8) pig oviduct tissue sample; (9) pig epididymis tissue sample.

    Article Snippet: The specificity of the primary antibody was confirmed by separate peptide competition assays; samples were incubated with the NHE1 antibody and its corresponding blocking peptide, which was five times in excess regarding the primary antibody.

    Techniques: Incubation, Blocking Assay

    Immunolocalization of NHE1 in the sperm plasma membrane ( A – C ), negative control ( D – F ), and after the peptide competition assay ( G – H ). NHE1 appears stained in green (FITC; fluorescein isothiocyanate) and nuclei are in blue (DAPI; 4′6′-diamidion-2-phenylindole). Scale bar: 9 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Blocking NHE Channels Reduces the Ability of In Vitro Capacitated Mammalian Sperm to Respond to Progesterone Stimulus

    doi: 10.3390/ijms222312646

    Figure Lengend Snippet: Immunolocalization of NHE1 in the sperm plasma membrane ( A – C ), negative control ( D – F ), and after the peptide competition assay ( G – H ). NHE1 appears stained in green (FITC; fluorescein isothiocyanate) and nuclei are in blue (DAPI; 4′6′-diamidion-2-phenylindole). Scale bar: 9 µm.

    Article Snippet: The specificity of the primary antibody was confirmed by separate peptide competition assays; samples were incubated with the NHE1 antibody and its corresponding blocking peptide, which was five times in excess regarding the primary antibody.

    Techniques: Negative Control, Competitive Binding Assay, Staining

    Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα ( a ), ENaCδ ( b ), and NHE1 ( c ) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca 2+ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. * p

    Journal: Scientific Reports

    Article Title: Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells

    doi: 10.1038/s41598-017-07756-x

    Figure Lengend Snippet: Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα ( a ), ENaCδ ( b ), and NHE1 ( c ) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca 2+ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. * p

    Article Snippet: The cells were fixed with 4% paraformaldehyde for 15 min at room temperature and then permeabilized with 10% methanol and 10% hydrogen peroxide in PBS for 15 min. Then cells were blocked with 3% goat serum, 3% bovine serum albumin in PBS for 1 h and stained with primary antibodies ENaCα and ENaCδ (LSBio) and NHE1 (Alomone Labs) overnight at 4 °C, followed by goat anti-rabbit secondary antibody (IgG Alexa 635, 1:500) for 30 min at room temperature.

    Techniques: Transfection, Concentration Assay

    ENaCα, ENaCδ, and NHE1 mRNA expression in both transfected and untransfected cells. mRNA expressions of ENaCα (a) , ENaCδ (b) , and NHE1 (c) were significantly decreased in siRNA-transfected cells. Each experiment was performed three times. * p

    Journal: Scientific Reports

    Article Title: Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells

    doi: 10.1038/s41598-017-07756-x

    Figure Lengend Snippet: ENaCα, ENaCδ, and NHE1 mRNA expression in both transfected and untransfected cells. mRNA expressions of ENaCα (a) , ENaCδ (b) , and NHE1 (c) were significantly decreased in siRNA-transfected cells. Each experiment was performed three times. * p

    Article Snippet: The cells were fixed with 4% paraformaldehyde for 15 min at room temperature and then permeabilized with 10% methanol and 10% hydrogen peroxide in PBS for 15 min. Then cells were blocked with 3% goat serum, 3% bovine serum albumin in PBS for 1 h and stained with primary antibodies ENaCα and ENaCδ (LSBio) and NHE1 (Alomone Labs) overnight at 4 °C, followed by goat anti-rabbit secondary antibody (IgG Alexa 635, 1:500) for 30 min at room temperature.

    Techniques: Expressing, Transfection

    ENaCα, ENaCδ, and NHE1 protein expression in transfected and untransfected cells. ENaCα, ENaCδ, and NHE1 are visualized with a red color; nuclei are stained with a blue color. (a) HBO cells express ENaCα proteins. (b) Percent cells expressing ENaCα in untransfected cells (30.2%, 88 of 291 cells), ENaCα siRNA transfected cells (1.5%, 5 of 327 cells), and scrambled siRNA- transfected cells (27.9%, 69 of 247 cells). (c) The ENaCδ proteins express in HBO cells. (d) Percent cells expressing ENaCδ in untransfected cells (27.8%, 49 of 176 cells), ENaCδ siRNA transfected cells (2.6%, 6 of 232 cells), and scrambled siRNA-transfected cells (22.5%, 29 of 129 cells). (e) Immunoreactivity of NHE1 antibodies demonstrates the presence of NHE1 expression in HBO cells. (f) Percent cells expressing NHE1 in untransfected cells (33.0%, 69 of 209 cells), NHE1 siRNA-transfected cells (1.8%, 5 of 280 cells), and scrambled siRNA-transfected cells (30.6%, 19 of 62 cells). Each experiment was performed three times. * p

    Journal: Scientific Reports

    Article Title: Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells

    doi: 10.1038/s41598-017-07756-x

    Figure Lengend Snippet: ENaCα, ENaCδ, and NHE1 protein expression in transfected and untransfected cells. ENaCα, ENaCδ, and NHE1 are visualized with a red color; nuclei are stained with a blue color. (a) HBO cells express ENaCα proteins. (b) Percent cells expressing ENaCα in untransfected cells (30.2%, 88 of 291 cells), ENaCα siRNA transfected cells (1.5%, 5 of 327 cells), and scrambled siRNA- transfected cells (27.9%, 69 of 247 cells). (c) The ENaCδ proteins express in HBO cells. (d) Percent cells expressing ENaCδ in untransfected cells (27.8%, 49 of 176 cells), ENaCδ siRNA transfected cells (2.6%, 6 of 232 cells), and scrambled siRNA-transfected cells (22.5%, 29 of 129 cells). (e) Immunoreactivity of NHE1 antibodies demonstrates the presence of NHE1 expression in HBO cells. (f) Percent cells expressing NHE1 in untransfected cells (33.0%, 69 of 209 cells), NHE1 siRNA-transfected cells (1.8%, 5 of 280 cells), and scrambled siRNA-transfected cells (30.6%, 19 of 62 cells). Each experiment was performed three times. * p

    Article Snippet: The cells were fixed with 4% paraformaldehyde for 15 min at room temperature and then permeabilized with 10% methanol and 10% hydrogen peroxide in PBS for 15 min. Then cells were blocked with 3% goat serum, 3% bovine serum albumin in PBS for 1 h and stained with primary antibodies ENaCα and ENaCδ (LSBio) and NHE1 (Alomone Labs) overnight at 4 °C, followed by goat anti-rabbit secondary antibody (IgG Alexa 635, 1:500) for 30 min at room temperature.

    Techniques: Expressing, Transfection, Staining