ns1619  (Alomone Labs)


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    Structured Review

    Alomone Labs ns1619
    Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or <t>NS1619</t> in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).
    Ns1619, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ns1619/product/Alomone Labs
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    ns1619 - by Bioz Stars, 2022-12
    92/100 stars

    Images

    1) Product Images from "Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration"

    Article Title: Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00210

    Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).
    Figure Legend Snippet: Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).

    Techniques Used: Migration, Control Assay, Positive Control

    2) Product Images from "Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration"

    Article Title: Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00210

    Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).
    Figure Legend Snippet: Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).

    Techniques Used: Migration, Control Assay, Positive Control

    3) Product Images from "Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration"

    Article Title: Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00210

    Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).
    Figure Legend Snippet: Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).

    Techniques Used: Migration, Control Assay, Positive Control

    4) Product Images from "Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration"

    Article Title: Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00210

    Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).
    Figure Legend Snippet: Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).

    Techniques Used: Migration, Control Assay, Positive Control

    5) Product Images from "Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration"

    Article Title: Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00210

    Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).
    Figure Legend Snippet: Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).

    Techniques Used: Migration, Control Assay, Positive Control

    6) Product Images from "Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration"

    Article Title: Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00210

    Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).
    Figure Legend Snippet: Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).

    Techniques Used: Migration, Control Assay, Positive Control

    7) Product Images from "Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration"

    Article Title: Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00210

    Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).
    Figure Legend Snippet: Effect of PL in rMSC migration. (A) rMSCs monolayers after scratching at 0 and 24 h in control cells and after treatment with IBTX (10 nM) or NS1619 in presence and absence of 5% PL. (B) Number of cells counted in wound area after each treatment was divided by number of cells in wound area in control assay. (C) Effect of PL in cell viability after 24 h of treatment with IBTX or NS1619 in the presence and absence of 5% PL. Doxorubicin 30 μM was used as a positive control. Data were normalized to control cells without treatment and shown as mean ± SEM ( n = 5).

    Techniques Used: Migration, Control Assay, Positive Control

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    Alomone Labs kca 3 1
    Potassium channels regulate the cellular internalization of various TAT-bound cargos. ( A ) TAT-PNA-induced luciferase activity in the indicated cell lines pretreated or not with potassium channel inhibitors (XE-991 or <t>TRAM-34)</t> or genetically invalidated for specific potassium channels. Results are normalized to non-stimulated cells (dashed lines). The independent experiment replicates are color-coded. The p-values correspond to the assessment of the significance of the differences with the control wild-type (WT) condition using ANOVA multiple comparison analysis with Dunnett’s correction. ( B ) Representative microscopy images of WT and KCNQ5 knock-out (KO) Raji cells expressing loxP-RFP-STOP-loxP-GFP and treated or not with 20 μM TAT-Cre for 48 hr. The results correspond to one of three independent experiments. ( C ) Internalization, recorded by flow cytometry, of FITC-D-JNKI1 after 1 hr of incubation in the indicated cell lines genetically invalidated (KO) or not (WT) for specific potassium channels. The results correspond to the median of three independent experiments.
    Kca 3 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kca 3 1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kca 3 1 - by Bioz Stars, 2022-12
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    94
    Alomone Labs anti cacnb2 antibody
    Potassium channels regulate the cellular internalization of various TAT-bound cargos. ( A ) TAT-PNA-induced luciferase activity in the indicated cell lines pretreated or not with potassium channel inhibitors (XE-991 or <t>TRAM-34)</t> or genetically invalidated for specific potassium channels. Results are normalized to non-stimulated cells (dashed lines). The independent experiment replicates are color-coded. The p-values correspond to the assessment of the significance of the differences with the control wild-type (WT) condition using ANOVA multiple comparison analysis with Dunnett’s correction. ( B ) Representative microscopy images of WT and KCNQ5 knock-out (KO) Raji cells expressing loxP-RFP-STOP-loxP-GFP and treated or not with 20 μM TAT-Cre for 48 hr. The results correspond to one of three independent experiments. ( C ) Internalization, recorded by flow cytometry, of FITC-D-JNKI1 after 1 hr of incubation in the indicated cell lines genetically invalidated (KO) or not (WT) for specific potassium channels. The results correspond to the median of three independent experiments.
    Anti Cacnb2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cacnb2 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cacnb2 antibody - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    Image Search Results


    Potassium channels regulate the cellular internalization of various TAT-bound cargos. ( A ) TAT-PNA-induced luciferase activity in the indicated cell lines pretreated or not with potassium channel inhibitors (XE-991 or TRAM-34) or genetically invalidated for specific potassium channels. Results are normalized to non-stimulated cells (dashed lines). The independent experiment replicates are color-coded. The p-values correspond to the assessment of the significance of the differences with the control wild-type (WT) condition using ANOVA multiple comparison analysis with Dunnett’s correction. ( B ) Representative microscopy images of WT and KCNQ5 knock-out (KO) Raji cells expressing loxP-RFP-STOP-loxP-GFP and treated or not with 20 μM TAT-Cre for 48 hr. The results correspond to one of three independent experiments. ( C ) Internalization, recorded by flow cytometry, of FITC-D-JNKI1 after 1 hr of incubation in the indicated cell lines genetically invalidated (KO) or not (WT) for specific potassium channels. The results correspond to the median of three independent experiments.

    Journal: eLife

    Article Title: Genetic, cellular, and structural characterization of the membrane potential-dependent cell-penetrating peptide translocation pore

    doi: 10.7554/eLife.69832

    Figure Lengend Snippet: Potassium channels regulate the cellular internalization of various TAT-bound cargos. ( A ) TAT-PNA-induced luciferase activity in the indicated cell lines pretreated or not with potassium channel inhibitors (XE-991 or TRAM-34) or genetically invalidated for specific potassium channels. Results are normalized to non-stimulated cells (dashed lines). The independent experiment replicates are color-coded. The p-values correspond to the assessment of the significance of the differences with the control wild-type (WT) condition using ANOVA multiple comparison analysis with Dunnett’s correction. ( B ) Representative microscopy images of WT and KCNQ5 knock-out (KO) Raji cells expressing loxP-RFP-STOP-loxP-GFP and treated or not with 20 μM TAT-Cre for 48 hr. The results correspond to one of three independent experiments. ( C ) Internalization, recorded by flow cytometry, of FITC-D-JNKI1 after 1 hr of incubation in the indicated cell lines genetically invalidated (KO) or not (WT) for specific potassium channels. The results correspond to the median of three independent experiments.

    Article Snippet: XE-991 and TRAM-34 (Alomone Labs, ref no. X-100 and T-105, respectively) was dissolved in DMSO at 100 mM and stored at –20°C.

    Techniques: Luciferase, Activity Assay, Microscopy, Knock-Out, Expressing, Flow Cytometry, Incubation

    Potassium channels modulate direct cell-penetrating peptide (CPP) translocation, but not endocytosis. ( A ) Same as Figure 2C , but for wild-type (WT), KCNN4 and KCNK5 SKW6.4 knock-out (KO) cells. The results correspond to the average of three independent experiments. ( B ) As panel A, but for WT and KCNN4 KO HeLa cells. The results correspond to the average of three independent experiments. ( C ) Quantitation by flow cytometry of 20 μg/ml AlexaFluor488-transferrin (left) or 200 μg/ml 10 kDa FITC-Dextran (right) internalization in the indicated WT cell lines and their corresponding KO versions, pretreated or not for 30 min with the XE-991 (10 μM) or TRAM-34 (10 μM) potassium channel inhibitors. Transferrin and dextran internalization was allowed to proceed for 60 min (still in the presence of inhibitors when these were used in the 30 min pre-incubation period). To quench membrane-bound fluorescence, cells were incubated with 0.2% trypan blue prior to flow cytometry analysis. The independent experiment replicates are color-coded. ( D ) Assessment of FITC-TAT-RasGAP 317-326 cell surface binding on WT and KCNQ5 KO Raji cells after 60 s of incubation (top), as well as associated peptide internalization after 1 hr of treatment (bottom). The results correspond to at least five independent experiments.

    Journal: eLife

    Article Title: Genetic, cellular, and structural characterization of the membrane potential-dependent cell-penetrating peptide translocation pore

    doi: 10.7554/eLife.69832

    Figure Lengend Snippet: Potassium channels modulate direct cell-penetrating peptide (CPP) translocation, but not endocytosis. ( A ) Same as Figure 2C , but for wild-type (WT), KCNN4 and KCNK5 SKW6.4 knock-out (KO) cells. The results correspond to the average of three independent experiments. ( B ) As panel A, but for WT and KCNN4 KO HeLa cells. The results correspond to the average of three independent experiments. ( C ) Quantitation by flow cytometry of 20 μg/ml AlexaFluor488-transferrin (left) or 200 μg/ml 10 kDa FITC-Dextran (right) internalization in the indicated WT cell lines and their corresponding KO versions, pretreated or not for 30 min with the XE-991 (10 μM) or TRAM-34 (10 μM) potassium channel inhibitors. Transferrin and dextran internalization was allowed to proceed for 60 min (still in the presence of inhibitors when these were used in the 30 min pre-incubation period). To quench membrane-bound fluorescence, cells were incubated with 0.2% trypan blue prior to flow cytometry analysis. The independent experiment replicates are color-coded. ( D ) Assessment of FITC-TAT-RasGAP 317-326 cell surface binding on WT and KCNQ5 KO Raji cells after 60 s of incubation (top), as well as associated peptide internalization after 1 hr of treatment (bottom). The results correspond to at least five independent experiments.

    Article Snippet: XE-991 and TRAM-34 (Alomone Labs, ref no. X-100 and T-105, respectively) was dissolved in DMSO at 100 mM and stored at –20°C.

    Techniques: Translocation Assay, Knock-Out, Quantitation Assay, Flow Cytometry, Incubation, Fluorescence, Binding Assay