p2x4  (Alomone Labs)


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    Structured Review

    Alomone Labs p2x4
    Effect of ethanol on the protein expression of P2X4Rs (A) and P2X7Rs (B) in BV2 cells. Upper panels — representative WBs demonstrating protein bands for the P2X4R (left), P2X7R (right) and Actin. Lower panels - densitometry analyses of the <t>P2X4</t> (left) or P2X7 (right) levels normalized to their corresponding actin levels. Changes were compared to the values for 0 ethanol which were arbitrarily adjusted to 1. Incubation of cells with ethanol for 24 h increased the expression of both P2XRs in a concentration-dependent manner. Significant differences were noted at 50 and 100 mM ethanol for both P2X4 and P2X7Rs. Data are presented as mean ± SEM (n = 5—7). *P
    P2x4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x4/product/Alomone Labs
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    p2x4 - by Bioz Stars, 2022-12
    92/100 stars

    Images

    1) Product Images from "Ethanol differentially modulates P2X4 and P2X7 receptor activity and function in BV2 microglial cells"

    Article Title: Ethanol differentially modulates P2X4 and P2X7 receptor activity and function in BV2 microglial cells

    Journal: Neuropharmacology

    doi: 10.1016/j.neuropharm.2017.09.030

    Effect of ethanol on the protein expression of P2X4Rs (A) and P2X7Rs (B) in BV2 cells. Upper panels — representative WBs demonstrating protein bands for the P2X4R (left), P2X7R (right) and Actin. Lower panels - densitometry analyses of the P2X4 (left) or P2X7 (right) levels normalized to their corresponding actin levels. Changes were compared to the values for 0 ethanol which were arbitrarily adjusted to 1. Incubation of cells with ethanol for 24 h increased the expression of both P2XRs in a concentration-dependent manner. Significant differences were noted at 50 and 100 mM ethanol for both P2X4 and P2X7Rs. Data are presented as mean ± SEM (n = 5—7). *P
    Figure Legend Snippet: Effect of ethanol on the protein expression of P2X4Rs (A) and P2X7Rs (B) in BV2 cells. Upper panels — representative WBs demonstrating protein bands for the P2X4R (left), P2X7R (right) and Actin. Lower panels - densitometry analyses of the P2X4 (left) or P2X7 (right) levels normalized to their corresponding actin levels. Changes were compared to the values for 0 ethanol which were arbitrarily adjusted to 1. Incubation of cells with ethanol for 24 h increased the expression of both P2XRs in a concentration-dependent manner. Significant differences were noted at 50 and 100 mM ethanol for both P2X4 and P2X7Rs. Data are presented as mean ± SEM (n = 5—7). *P

    Techniques Used: Expressing, Incubation, Concentration Assay

    2) Product Images from "Primary cilia mediate mechanotransduction through control of ATP-induced Ca2+ signaling in compressed chondrocytes"

    Article Title: Primary cilia mediate mechanotransduction through control of ATP-induced Ca2+ signaling in compressed chondrocytes

    Journal: The FASEB Journal

    doi: 10.1096/fj.11-193649

    P2, PC1, and PC2 expression in WT and ORPK cells. A ) Western blots of P2 receptors, P2X4, P2X7, P2Y1, and P2Y2. B ) Quantification of P2 receptor expression reveals no significant differences between WT and ORPK chondrocytes. Data were normalized to β-actin.
    Figure Legend Snippet: P2, PC1, and PC2 expression in WT and ORPK cells. A ) Western blots of P2 receptors, P2X4, P2X7, P2Y1, and P2Y2. B ) Quantification of P2 receptor expression reveals no significant differences between WT and ORPK chondrocytes. Data were normalized to β-actin.

    Techniques Used: Expressing, Western Blot

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    • p2x4  (Alomone Labs)
    92
    Alomone Labs p2x4
    Effect of ethanol on the protein expression of P2X4Rs (A) and P2X7Rs (B) in BV2 cells. Upper panels — representative WBs demonstrating protein bands for the P2X4R (left), P2X7R (right) and Actin. Lower panels - densitometry analyses of the <t>P2X4</t> (left) or P2X7 (right) levels normalized to their corresponding actin levels. Changes were compared to the values for 0 ethanol which were arbitrarily adjusted to 1. Incubation of cells with ethanol for 24 h increased the expression of both P2XRs in a concentration-dependent manner. Significant differences were noted at 50 and 100 mM ethanol for both P2X4 and P2X7Rs. Data are presented as mean ± SEM (n = 5—7). *P
    P2x4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x4/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2x4 - by Bioz Stars, 2022-12
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of ethanol on the protein expression of P2X4Rs (A) and P2X7Rs (B) in BV2 cells. Upper panels — representative WBs demonstrating protein bands for the P2X4R (left), P2X7R (right) and Actin. Lower panels - densitometry analyses of the P2X4 (left) or P2X7 (right) levels normalized to their corresponding actin levels. Changes were compared to the values for 0 ethanol which were arbitrarily adjusted to 1. Incubation of cells with ethanol for 24 h increased the expression of both P2XRs in a concentration-dependent manner. Significant differences were noted at 50 and 100 mM ethanol for both P2X4 and P2X7Rs. Data are presented as mean ± SEM (n = 5—7). *P

    Journal: Neuropharmacology

    Article Title: Ethanol differentially modulates P2X4 and P2X7 receptor activity and function in BV2 microglial cells

    doi: 10.1016/j.neuropharm.2017.09.030

    Figure Lengend Snippet: Effect of ethanol on the protein expression of P2X4Rs (A) and P2X7Rs (B) in BV2 cells. Upper panels — representative WBs demonstrating protein bands for the P2X4R (left), P2X7R (right) and Actin. Lower panels - densitometry analyses of the P2X4 (left) or P2X7 (right) levels normalized to their corresponding actin levels. Changes were compared to the values for 0 ethanol which were arbitrarily adjusted to 1. Incubation of cells with ethanol for 24 h increased the expression of both P2XRs in a concentration-dependent manner. Significant differences were noted at 50 and 100 mM ethanol for both P2X4 and P2X7Rs. Data are presented as mean ± SEM (n = 5—7). *P

    Article Snippet: Antibodies to P2X4 and P2X7 proteins were purchased from Alomone Labs (Jerusalem, Israel), antibody to beta actin — from Sigma Co. All other chemicals were of reagent grade.

    Techniques: Expressing, Incubation, Concentration Assay

    P2, PC1, and PC2 expression in WT and ORPK cells. A ) Western blots of P2 receptors, P2X4, P2X7, P2Y1, and P2Y2. B ) Quantification of P2 receptor expression reveals no significant differences between WT and ORPK chondrocytes. Data were normalized to β-actin.

    Journal: The FASEB Journal

    Article Title: Primary cilia mediate mechanotransduction through control of ATP-induced Ca2+ signaling in compressed chondrocytes

    doi: 10.1096/fj.11-193649

    Figure Lengend Snippet: P2, PC1, and PC2 expression in WT and ORPK cells. A ) Western blots of P2 receptors, P2X4, P2X7, P2Y1, and P2Y2. B ) Quantification of P2 receptor expression reveals no significant differences between WT and ORPK chondrocytes. Data were normalized to β-actin.

    Article Snippet: The study used the following antibodies: P2X2, P2X4, P2X7, P2Y1, and P2Y2 (Alomone Laboratories, Jerusalem, Israel); acetylated α-tubulin (clone 6-11B-1, Sigma, Auckland, New Zealand; and C3B9, gift from T. Sherwin, University of Auckland); large T-antigen SV40 (BD Biosciences, Auckland, New Zealand); polycystin-1 (PC1; H-260) and polycystin-2 (PC2; H-280; Santa Cruz Biotechnologies, Santa Cruz, CA, USA); goat anti-rabbit Dylight 488 and goat anti-mouse Dylight 594 (Jackson ImmunoResearch, West Grove, PA, USA); and sheep anti-rabbit horseradish peroxidase (Chemicon, Auckland, New Zealand).

    Techniques: Expressing, Western Blot