p2x4 antibody (Alomone Labs)

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Name:

Anti P2X4 Receptor Antibody

Description:

Anti P2X4 Receptor Antibody APR 002 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunocytochemistry immunohistochemistry immunoprecipitation and indirect flow cytometry applications It has been designed to recognize P2X4 purinergic receptor from rat mouse and human samples

Catalog Number:

APR-002

Price:

397.0

Category:

Primary Antibody

Applications:

Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity:

Affinity purified on immobilized antigen.

Immunogen:

Synthetic peptide

Size:

25 mcl

Antibody Type:

Polyclonal Primary Antibodies

Format:

Lyophilized Powder

Host:

Rabbit

Isotype:

Rabbit IgG

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Structured Review

Alomone Labs p2x4 antibody

p2x4 antibody - by Bioz Stars, 2021-09

95/100 stars

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Incubation:

Article Title: Cystitis-Related Bladder Pain Involves ATP-Dependent HMGB1 Release from Macrophages and Its Downstream H2S/Cav3.2 Signaling in Mice
Article Snippet: .. After incubation for 48 h, protein levels of P2X4 or P2X7 were determined by Western blotting, using an anti-P2X4 rabbit polyclonal antibody (Alomone Labs) (1:1000 dilution) and an anti-P2X7 rabbit polyclonal antibody (alomone labs) (1:3000 dilution) as the primary antibodies, and an HRP-conjugated anti-rabbit IgG antibody (Chemicon International) (1:5000 dilution) as the secondary antibody. ..

Article Title: Inhibition of P2X4R attenuates white matter injury in mice after intracerebral hemorrhage by regulating microglial phenotypes
Article Snippet: .. The membranes were blocked with 5% milk at room temperature for 1 h and incubated overnight with the following primary antibodies: P2X4R (1:1000; APR-002; Alomone), MBP (1:1000; CST#83683S; Cell Signaling Technology), amyloid precursor protein (APP) (1:1000; 32136; Abcam), BDNF (1:1000; 28205-1-AP; Proteintech), p-TrkB (1:1000; ABN1381; Millipore), TrkB (1:100; sc-7268; Santa Cruz), P2X7R (1:1000; APR-004; Alomone), and β-actin (1:5000, 8226; Abcam). ..

Article Title: Astragalin Alleviates Neuropathic Pain by Suppressing P2X4-Mediated Signaling in the Dorsal Root Ganglia of Rats
Article Snippet: .. Sections were incubated overnight at 4°C with mouse anti-GFAP (1:200 dilution; 38014, Signalway Antibody, Nanjing, China) and rabbit anti-P2X4 (1:200; APR-002, Alomone Labs, Jerusalem, Israel) primary antibodies and washed three times for 10 min each in PBS ( ). ..

Article Title: P2X4 Purinergic Receptors as a Therapeutic Target in Aggressive Prostate Cancer
Article Snippet: .. Slides were steamed for 25 minutes in antigen retrieval solution (Cat. No., H-3300, Vector Laboratories, INC. Burlingame, CA) and incubated with rabbit polyclonal anti-human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal anti-mouse P2X4 (Cat. No. APR-002, Alomone Labs, Jerusalem, Israel), rabbit monoclonal anti-androgen receptor antibody (Cat. No. 5153, Cell Signaling, Danvers, MA), rabbit monoclonal anti-CD11b (Cat. No. 6410, Bio SB, Santa Barbara, CA.), or rabbit monoclonal anti-CD3 (Cat. No. RM-9107-S0, ThermoFisher Scientific, Waltham, MA) for 45 minutes at RT. ..

Article Title: Inhibition of P2X4R attenuates white matter injury in mice after intracerebral hemorrhage by regulating microglial phenotypes
Article Snippet: .. After washing with PBS and incubation with 10% donkey serum containing 0.3% Triton X-100 for 1 h at room temperature, the sections were incubated with primary antibodies overnight at 4 °C, including P2X4R (1:200, Alomone, APR-002), Iba-1 (1:500, Abcam, 5076), NeuN (1:1000, Abcam, 177487), GFAP (1:500, Millipore, MAB360), MBP (1:500, Cell Signaling Technology, CST#83683S), Neurofilament-200 (NF-200) (1:1000, Sigma, N4142), CD16/32 (1:100, Abcam, 24235), and Arg-1(1:500, Proteintech, 16001-1-AP). ..

Article Title: Astragalin Alleviates Neuropathic Pain by Suppressing P2X4-Mediated Signaling in the Dorsal Root Ganglia of Rats
Article Snippet: .. Non-specific antigens were blocked by incubating the membrane for 2 h at room temperature in 5% skim milk; this was followed by overnight incubation at 4°C with the following primary antibodies: rabbit anti-P2X4 (1:500; Alomone Labs), rabbit anti-ERK1/2 (1:1,000), and anti-phosphorylated (p)ERK1/2 (1:2,000) (both from Cell Signaling Technology, Danvers, MA, United States); and rabbit anti-tumor necrosis factor receptor (TNF-R)1 (1:800) and mouse anti-GFAP (1:800) (both from Abcam, Cambridge, MA, United States). ..

Western Blot:

Binding Assay:

Article Title: P2X4 Purinergic Receptors as a Therapeutic Target in Aggressive Prostate Cancer
Article Snippet: .. Blots were probed with antibody specific for human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), mouse P2X4 (Cat. No. APR-002, Alomone Labs, Jerusalem, Israel), E-cadherin (Cat. No. 3195, Cell Signaling, Danvers, MA.), Vimentin (Cat. No. 5741, Cell Signaling, Danvers, MA), and cleaved caspase-3 (Cat. No. 9664, Cell Signaling, Danvers, MA). β-Actin (Cat. No. A5441, Sigma-Aldrich, St. Louis, MO) or TATA binding protein (Cat. No. 51841, Abcam, Cambridge, MA) were used to ensure equal loading of proteins in each lane. ..

other:

Article Title: Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia, et al. Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia
Article Snippet: Validation of polyclonal rabbit anti‐P2X4 antibody.