anti slc4a4 rabbit  (Alomone Labs)


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    Alomone Labs anti slc4a4 rabbit
    Evaluation of NBCe1 transport activity. Calibration of the H + ‐sensitive fluorescence signal in cultured cortical astrocytes. Calibration of the fluorescence intensity (F.I) 440/488 ratio signal in the presence of nigericin to equilibrate H + across the cell membrane, monitored at different extracellular pH values, 6.5, 7.0, 7.5, 8.0, and plotted against the pH value and the respective H + concentration. The coefficient of correlation (R2) and the fit equation are indicated in the plot. (b): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, to challenge outwardly directed NBCe1 activity, before and after incubation with 4‐AP (100 µM, 20 min), in control, and in the presence of the TGF‐β receptor II inhibitor SB431542 (10 µM) (b–e). Bar plots of the rate of acidification (c), the rate of alkalinisation (d), and the amplitude (e) as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. (f): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes from wild type (black; WT) and <t>Slc4a4</t> deficient mice (red; NBCe1‐KO) during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, before and after incubation with 2 ng/mL TGF‐β for 60 min. (g): Bar plots of the rate of acidification, and the rate of alkalinisation as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. * p
    Anti Slc4a4 Rabbit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti slc4a4 rabbit/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti slc4a4 rabbit - by Bioz Stars, 2022-05
    93/100 stars

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    1) Product Images from "TGF‐β signaling directly regulates transcription and functional expression of the electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), via Smad4 in mouse astrocytes, et al. TGF‐β signaling directly regulates transcription and functional expression of the electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), via Smad4 in mouse astrocytes"

    Article Title: TGF‐β signaling directly regulates transcription and functional expression of the electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), via Smad4 in mouse astrocytes, et al. TGF‐β signaling directly regulates transcription and functional expression of the electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), via Smad4 in mouse astrocytes

    Journal: Glia

    doi: 10.1002/glia.23168

    Evaluation of NBCe1 transport activity. Calibration of the H + ‐sensitive fluorescence signal in cultured cortical astrocytes. Calibration of the fluorescence intensity (F.I) 440/488 ratio signal in the presence of nigericin to equilibrate H + across the cell membrane, monitored at different extracellular pH values, 6.5, 7.0, 7.5, 8.0, and plotted against the pH value and the respective H + concentration. The coefficient of correlation (R2) and the fit equation are indicated in the plot. (b): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, to challenge outwardly directed NBCe1 activity, before and after incubation with 4‐AP (100 µM, 20 min), in control, and in the presence of the TGF‐β receptor II inhibitor SB431542 (10 µM) (b–e). Bar plots of the rate of acidification (c), the rate of alkalinisation (d), and the amplitude (e) as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. (f): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes from wild type (black; WT) and Slc4a4 deficient mice (red; NBCe1‐KO) during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, before and after incubation with 2 ng/mL TGF‐β for 60 min. (g): Bar plots of the rate of acidification, and the rate of alkalinisation as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. * p
    Figure Legend Snippet: Evaluation of NBCe1 transport activity. Calibration of the H + ‐sensitive fluorescence signal in cultured cortical astrocytes. Calibration of the fluorescence intensity (F.I) 440/488 ratio signal in the presence of nigericin to equilibrate H + across the cell membrane, monitored at different extracellular pH values, 6.5, 7.0, 7.5, 8.0, and plotted against the pH value and the respective H + concentration. The coefficient of correlation (R2) and the fit equation are indicated in the plot. (b): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, to challenge outwardly directed NBCe1 activity, before and after incubation with 4‐AP (100 µM, 20 min), in control, and in the presence of the TGF‐β receptor II inhibitor SB431542 (10 µM) (b–e). Bar plots of the rate of acidification (c), the rate of alkalinisation (d), and the amplitude (e) as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. (f): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes from wild type (black; WT) and Slc4a4 deficient mice (red; NBCe1‐KO) during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, before and after incubation with 2 ng/mL TGF‐β for 60 min. (g): Bar plots of the rate of acidification, and the rate of alkalinisation as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. * p

    Techniques Used: Activity Assay, Fluorescence, Cell Culture, Concentration Assay, Incubation, Mouse Assay

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    Alomone Labs anti slc4a4 rabbit
    Evaluation of NBCe1 transport activity. Calibration of the H + ‐sensitive fluorescence signal in cultured cortical astrocytes. Calibration of the fluorescence intensity (F.I) 440/488 ratio signal in the presence of nigericin to equilibrate H + across the cell membrane, monitored at different extracellular pH values, 6.5, 7.0, 7.5, 8.0, and plotted against the pH value and the respective H + concentration. The coefficient of correlation (R2) and the fit equation are indicated in the plot. (b): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, to challenge outwardly directed NBCe1 activity, before and after incubation with 4‐AP (100 µM, 20 min), in control, and in the presence of the TGF‐β receptor II inhibitor SB431542 (10 µM) (b–e). Bar plots of the rate of acidification (c), the rate of alkalinisation (d), and the amplitude (e) as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. (f): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes from wild type (black; WT) and <t>Slc4a4</t> deficient mice (red; NBCe1‐KO) during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, before and after incubation with 2 ng/mL TGF‐β for 60 min. (g): Bar plots of the rate of acidification, and the rate of alkalinisation as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. * p
    Anti Slc4a4 Rabbit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti slc4a4 rabbit/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti slc4a4 rabbit - by Bioz Stars, 2022-05
    93/100 stars
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    Evaluation of NBCe1 transport activity. Calibration of the H + ‐sensitive fluorescence signal in cultured cortical astrocytes. Calibration of the fluorescence intensity (F.I) 440/488 ratio signal in the presence of nigericin to equilibrate H + across the cell membrane, monitored at different extracellular pH values, 6.5, 7.0, 7.5, 8.0, and plotted against the pH value and the respective H + concentration. The coefficient of correlation (R2) and the fit equation are indicated in the plot. (b): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, to challenge outwardly directed NBCe1 activity, before and after incubation with 4‐AP (100 µM, 20 min), in control, and in the presence of the TGF‐β receptor II inhibitor SB431542 (10 µM) (b–e). Bar plots of the rate of acidification (c), the rate of alkalinisation (d), and the amplitude (e) as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. (f): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes from wild type (black; WT) and Slc4a4 deficient mice (red; NBCe1‐KO) during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, before and after incubation with 2 ng/mL TGF‐β for 60 min. (g): Bar plots of the rate of acidification, and the rate of alkalinisation as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. * p

    Journal: Glia

    Article Title: TGF‐β signaling directly regulates transcription and functional expression of the electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), via Smad4 in mouse astrocytes, et al. TGF‐β signaling directly regulates transcription and functional expression of the electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), via Smad4 in mouse astrocytes

    doi: 10.1002/glia.23168

    Figure Lengend Snippet: Evaluation of NBCe1 transport activity. Calibration of the H + ‐sensitive fluorescence signal in cultured cortical astrocytes. Calibration of the fluorescence intensity (F.I) 440/488 ratio signal in the presence of nigericin to equilibrate H + across the cell membrane, monitored at different extracellular pH values, 6.5, 7.0, 7.5, 8.0, and plotted against the pH value and the respective H + concentration. The coefficient of correlation (R2) and the fit equation are indicated in the plot. (b): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, to challenge outwardly directed NBCe1 activity, before and after incubation with 4‐AP (100 µM, 20 min), in control, and in the presence of the TGF‐β receptor II inhibitor SB431542 (10 µM) (b–e). Bar plots of the rate of acidification (c), the rate of alkalinisation (d), and the amplitude (e) as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. (f): Original recordings of intracellular [H + ] ([H + ] i ) in cultured cortical astrocytes from wild type (black; WT) and Slc4a4 deficient mice (red; NBCe1‐KO) during reduction of external pH and [ HCO 3 − ] from 7.4 and 26 mM to 7.1 and 13 mM, respectively, before and after incubation with 2 ng/mL TGF‐β for 60 min. (g): Bar plots of the rate of acidification, and the rate of alkalinisation as measured upon changing external pH and [ HCO 3 − ] to 7.1 and 13 mM, respectively, and back to pH 7.4 and 26 mM [ HCO 3 − ]. * p

    Article Snippet: 2.1 Antibodies and reagents/chemicals Following antibodies were used as primary antibodies: anti‐SLC4A4 rabbit polyclonal from Alomone labs, (Jerusalem, Israel) for western blots; from Atlas Antibodies (Bromma, Sweden) for immunocytochemistry.

    Techniques: Activity Assay, Fluorescence, Cell Culture, Concentration Assay, Incubation, Mouse Assay