anti na k cl cotransporter type 2  (Alomone Labs)


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    Alomone Labs anti na k cl cotransporter type 2
    Expression of major Na + transporting systems in distal tubule and CD in water-deprived Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of 24 h water-deprived Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against Na + /K + /Cl − <t>cotransporter</t> SLC12A1 , NKCC2 (top) ,and phospho-pT 96 T 101 NKCC2 (bottom) forms. B , C ) Summary graph comparing total renal NKCC2 expression ( B ) and phosphorylated pT 96 T 101 NKCC2 levels ( C ) in Epac WT, Epac1 −/− , and Epac2 −/− mice from Western blots similar to that shown in A . D ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against phosphorylated pT 53 form of thiazide-sensitive Na + /Cl − cotransporter SLC12A3 , NCC (top), and full-length αENaC (bottom). E , F ) Summary graph comparing total renal pT 53 NCC expression ( E ) and αENaC ( F ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in D . Intensity values were normalized to total protein signal after Ponceau red staining. * P
    Anti Na K Cl Cotransporter Type 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti na k cl cotransporter type 2/product/Alomone Labs
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti na k cl cotransporter type 2 - by Bioz Stars, 2022-08
    91/100 stars

    Images

    1) Product Images from "Urinary concentrating defect in mice lacking Epac1 or Epac2"

    Article Title: Urinary concentrating defect in mice lacking Epac1 or Epac2

    Journal: The FASEB Journal

    doi: 10.1096/fj.201800435R

    Expression of major Na + transporting systems in distal tubule and CD in water-deprived Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of 24 h water-deprived Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against Na + /K + /Cl − cotransporter SLC12A1 , NKCC2 (top) ,and phospho-pT 96 T 101 NKCC2 (bottom) forms. B , C ) Summary graph comparing total renal NKCC2 expression ( B ) and phosphorylated pT 96 T 101 NKCC2 levels ( C ) in Epac WT, Epac1 −/− , and Epac2 −/− mice from Western blots similar to that shown in A . D ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against phosphorylated pT 53 form of thiazide-sensitive Na + /Cl − cotransporter SLC12A3 , NCC (top), and full-length αENaC (bottom). E , F ) Summary graph comparing total renal pT 53 NCC expression ( E ) and αENaC ( F ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in D . Intensity values were normalized to total protein signal after Ponceau red staining. * P
    Figure Legend Snippet: Expression of major Na + transporting systems in distal tubule and CD in water-deprived Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of 24 h water-deprived Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against Na + /K + /Cl − cotransporter SLC12A1 , NKCC2 (top) ,and phospho-pT 96 T 101 NKCC2 (bottom) forms. B , C ) Summary graph comparing total renal NKCC2 expression ( B ) and phosphorylated pT 96 T 101 NKCC2 levels ( C ) in Epac WT, Epac1 −/− , and Epac2 −/− mice from Western blots similar to that shown in A . D ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against phosphorylated pT 53 form of thiazide-sensitive Na + /Cl − cotransporter SLC12A3 , NCC (top), and full-length αENaC (bottom). E , F ) Summary graph comparing total renal pT 53 NCC expression ( E ) and αENaC ( F ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in D . Intensity values were normalized to total protein signal after Ponceau red staining. * P

    Techniques Used: Expressing, Mouse Assay, Western Blot, Staining

    Expression of major Na + transporting systems in distal tubule and CD in Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against Na + /K + /Cl − cotransporter SLC12A1 , NKCC2 (top), and phospho-pT 96 T 101 NKCC2 (bottom) forms. B , C ) Summary graph comparing total renal NKCC2 expression ( B ) and phosphorylated pT 96 T 101 NKCC2 levels ( C ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in A . D ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against phosphorylated pT 53 form of thiazide-sensitive Na + /Cl − cotransporter SLC12A3 , NCC (top), and full-length αENaC (bottom). E , F ) Summary graph comparing total renal pT 53 NCC expression ( E ) and αENaC ( F ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in D . Intensity values were normalized to total protein signal after Ponceau red staining. * P
    Figure Legend Snippet: Expression of major Na + transporting systems in distal tubule and CD in Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against Na + /K + /Cl − cotransporter SLC12A1 , NKCC2 (top), and phospho-pT 96 T 101 NKCC2 (bottom) forms. B , C ) Summary graph comparing total renal NKCC2 expression ( B ) and phosphorylated pT 96 T 101 NKCC2 levels ( C ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in A . D ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against phosphorylated pT 53 form of thiazide-sensitive Na + /Cl − cotransporter SLC12A3 , NCC (top), and full-length αENaC (bottom). E , F ) Summary graph comparing total renal pT 53 NCC expression ( E ) and αENaC ( F ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in D . Intensity values were normalized to total protein signal after Ponceau red staining. * P

    Techniques Used: Expressing, Mouse Assay, Western Blot, Staining

    2) Product Images from "Octopus Cells in the Posteroventral Cochlear Nucleus Provide the Main Excitatory Input to the Superior Paraolivary Nucleus"

    Article Title: Octopus Cells in the Posteroventral Cochlear Nucleus Provide the Main Excitatory Input to the Superior Paraolivary Nucleus

    Journal: Frontiers in Neural Circuits

    doi: 10.3389/fncir.2017.00037

    Immunolabeling of calretinin reveals the trajectory of the intermediate acoustic stria (IAS) from the posteroventral cochlear nucleus (PVCN) to the contralateral superior olivary complex. (A) Micrograph depicting the cell bodies of octopus cells densely immunolabeled for calretinin (asterisks). (B) The cell bodies of neurons of the anteroventral cochlear nucleus (AVCN) are immunonegative for calretinin and are surrounded by abundant calretinin-positive terminals. (C) A dense plexus of calretinin-positive fibers that presumably originate from the contralateral IAS innervates the superior paraolivary nucleus (SPON). Notice that areas bordering the SPON that correspond to the medial nucleus of the trapezoid body (MNTB) and the lateral superior olive (LSO) are largely devoid of labeled fibers. (D) Micrograph of the ventral portion of the IAS, as it approaches its ipsilateral SOC. The thick, calretinin-positive axons presumably belong to octopus cells. (E) Co-staining of calretinin-positive fibers (red) and the postsynaptic marker potassium-chloride cotransporter 2 (KCC2; green) indicates that the SPON is densely innervated by calretinin-positive fibers, which presumably belong to octopus cells. (F) Co-staining of calretinin and KCC2 also demonstrates large calretinin-immunolabeled calyx-like synaptic specializations, which presumably belong to octopus cells, surrounding the cell bodies of neurons of the ventral nucleus of the lateral lemniscus (VNLL). The arrowheads indicate calretinin-positive axons approaching the VNLL. Orientation arrows in D also apply to (A,B) . Orientation arrows in (C) also apply to (E) . Calibration bar in (D) also applies to (C) .
    Figure Legend Snippet: Immunolabeling of calretinin reveals the trajectory of the intermediate acoustic stria (IAS) from the posteroventral cochlear nucleus (PVCN) to the contralateral superior olivary complex. (A) Micrograph depicting the cell bodies of octopus cells densely immunolabeled for calretinin (asterisks). (B) The cell bodies of neurons of the anteroventral cochlear nucleus (AVCN) are immunonegative for calretinin and are surrounded by abundant calretinin-positive terminals. (C) A dense plexus of calretinin-positive fibers that presumably originate from the contralateral IAS innervates the superior paraolivary nucleus (SPON). Notice that areas bordering the SPON that correspond to the medial nucleus of the trapezoid body (MNTB) and the lateral superior olive (LSO) are largely devoid of labeled fibers. (D) Micrograph of the ventral portion of the IAS, as it approaches its ipsilateral SOC. The thick, calretinin-positive axons presumably belong to octopus cells. (E) Co-staining of calretinin-positive fibers (red) and the postsynaptic marker potassium-chloride cotransporter 2 (KCC2; green) indicates that the SPON is densely innervated by calretinin-positive fibers, which presumably belong to octopus cells. (F) Co-staining of calretinin and KCC2 also demonstrates large calretinin-immunolabeled calyx-like synaptic specializations, which presumably belong to octopus cells, surrounding the cell bodies of neurons of the ventral nucleus of the lateral lemniscus (VNLL). The arrowheads indicate calretinin-positive axons approaching the VNLL. Orientation arrows in D also apply to (A,B) . Orientation arrows in (C) also apply to (E) . Calibration bar in (D) also applies to (C) .

    Techniques Used: Immunolabeling, Labeling, Staining, Marker

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    Alomone Labs anti na k cl cotransporter type 2
    Expression of major Na + transporting systems in distal tubule and CD in water-deprived Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of 24 h water-deprived Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against Na + /K + /Cl − <t>cotransporter</t> SLC12A1 , NKCC2 (top) ,and phospho-pT 96 T 101 NKCC2 (bottom) forms. B , C ) Summary graph comparing total renal NKCC2 expression ( B ) and phosphorylated pT 96 T 101 NKCC2 levels ( C ) in Epac WT, Epac1 −/− , and Epac2 −/− mice from Western blots similar to that shown in A . D ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against phosphorylated pT 53 form of thiazide-sensitive Na + /Cl − cotransporter SLC12A3 , NCC (top), and full-length αENaC (bottom). E , F ) Summary graph comparing total renal pT 53 NCC expression ( E ) and αENaC ( F ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in D . Intensity values were normalized to total protein signal after Ponceau red staining. * P
    Anti Na K Cl Cotransporter Type 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti na k cl cotransporter type 2/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti na k cl cotransporter type 2 - by Bioz Stars, 2022-08
    91/100 stars
      Buy from Supplier

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    Expression of major Na + transporting systems in distal tubule and CD in water-deprived Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of 24 h water-deprived Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against Na + /K + /Cl − cotransporter SLC12A1 , NKCC2 (top) ,and phospho-pT 96 T 101 NKCC2 (bottom) forms. B , C ) Summary graph comparing total renal NKCC2 expression ( B ) and phosphorylated pT 96 T 101 NKCC2 levels ( C ) in Epac WT, Epac1 −/− , and Epac2 −/− mice from Western blots similar to that shown in A . D ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against phosphorylated pT 53 form of thiazide-sensitive Na + /Cl − cotransporter SLC12A3 , NCC (top), and full-length αENaC (bottom). E , F ) Summary graph comparing total renal pT 53 NCC expression ( E ) and αENaC ( F ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in D . Intensity values were normalized to total protein signal after Ponceau red staining. * P

    Journal: The FASEB Journal

    Article Title: Urinary concentrating defect in mice lacking Epac1 or Epac2

    doi: 10.1096/fj.201800435R

    Figure Lengend Snippet: Expression of major Na + transporting systems in distal tubule and CD in water-deprived Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of 24 h water-deprived Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against Na + /K + /Cl − cotransporter SLC12A1 , NKCC2 (top) ,and phospho-pT 96 T 101 NKCC2 (bottom) forms. B , C ) Summary graph comparing total renal NKCC2 expression ( B ) and phosphorylated pT 96 T 101 NKCC2 levels ( C ) in Epac WT, Epac1 −/− , and Epac2 −/− mice from Western blots similar to that shown in A . D ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against phosphorylated pT 53 form of thiazide-sensitive Na + /Cl − cotransporter SLC12A3 , NCC (top), and full-length αENaC (bottom). E , F ) Summary graph comparing total renal pT 53 NCC expression ( E ) and αENaC ( F ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in D . Intensity values were normalized to total protein signal after Ponceau red staining. * P

    Article Snippet: We used the primary antibodies against anti-AQP2 (1:1000, AQP2-002; Alomone Labs, Jerusalem, Israel), anti–Na+ -K+ -Cl− cotransporter type 2 ( SLC12A1 ; NKCC2; rabbit polyclonal, 1:1000, ANT-072; Alomone Labs), anti–NHE-3, pS552 NHE-3 (mouse monoclonal, 1:100, sc-136368, sc-53962; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Na+ /H+ exchanger regulatory factor isoform 1 (NHERF1; mouse monoclonal, 1:1000, sc-271552; Santa Cruz Biotechnology), anti-pT96 T101 NKCC2 (rabbit polyclonal, 1:2000, gift of B. Forbush, Yale University, New Haven, CT, USA), anti-pT53 thiazide-sensitive Na+ -Cl− cotransporter ( SLC12A3 ) (NCC; rabbit polyclonal, 1:1000; p1311-53; PhosphoSolutions, Aurora, CO, USA), anti–α subunit of epithelial Na+ ).

    Techniques: Expressing, Mouse Assay, Western Blot, Staining

    Expression of major Na + transporting systems in distal tubule and CD in Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against Na + /K + /Cl − cotransporter SLC12A1 , NKCC2 (top), and phospho-pT 96 T 101 NKCC2 (bottom) forms. B , C ) Summary graph comparing total renal NKCC2 expression ( B ) and phosphorylated pT 96 T 101 NKCC2 levels ( C ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in A . D ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against phosphorylated pT 53 form of thiazide-sensitive Na + /Cl − cotransporter SLC12A3 , NCC (top), and full-length αENaC (bottom). E , F ) Summary graph comparing total renal pT 53 NCC expression ( E ) and αENaC ( F ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in D . Intensity values were normalized to total protein signal after Ponceau red staining. * P

    Journal: The FASEB Journal

    Article Title: Urinary concentrating defect in mice lacking Epac1 or Epac2

    doi: 10.1096/fj.201800435R

    Figure Lengend Snippet: Expression of major Na + transporting systems in distal tubule and CD in Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against Na + /K + /Cl − cotransporter SLC12A1 , NKCC2 (top), and phospho-pT 96 T 101 NKCC2 (bottom) forms. B , C ) Summary graph comparing total renal NKCC2 expression ( B ) and phosphorylated pT 96 T 101 NKCC2 levels ( C ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in A . D ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with antibodies against phosphorylated pT 53 form of thiazide-sensitive Na + /Cl − cotransporter SLC12A3 , NCC (top), and full-length αENaC (bottom). E , F ) Summary graph comparing total renal pT 53 NCC expression ( E ) and αENaC ( F ) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in D . Intensity values were normalized to total protein signal after Ponceau red staining. * P

    Article Snippet: We used the primary antibodies against anti-AQP2 (1:1000, AQP2-002; Alomone Labs, Jerusalem, Israel), anti–Na+ -K+ -Cl− cotransporter type 2 ( SLC12A1 ; NKCC2; rabbit polyclonal, 1:1000, ANT-072; Alomone Labs), anti–NHE-3, pS552 NHE-3 (mouse monoclonal, 1:100, sc-136368, sc-53962; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Na+ /H+ exchanger regulatory factor isoform 1 (NHERF1; mouse monoclonal, 1:1000, sc-271552; Santa Cruz Biotechnology), anti-pT96 T101 NKCC2 (rabbit polyclonal, 1:2000, gift of B. Forbush, Yale University, New Haven, CT, USA), anti-pT53 thiazide-sensitive Na+ -Cl− cotransporter ( SLC12A3 ) (NCC; rabbit polyclonal, 1:1000; p1311-53; PhosphoSolutions, Aurora, CO, USA), anti–α subunit of epithelial Na+ ).

    Techniques: Expressing, Mouse Assay, Western Blot, Staining

    Immunolabeling of calretinin reveals the trajectory of the intermediate acoustic stria (IAS) from the posteroventral cochlear nucleus (PVCN) to the contralateral superior olivary complex. (A) Micrograph depicting the cell bodies of octopus cells densely immunolabeled for calretinin (asterisks). (B) The cell bodies of neurons of the anteroventral cochlear nucleus (AVCN) are immunonegative for calretinin and are surrounded by abundant calretinin-positive terminals. (C) A dense plexus of calretinin-positive fibers that presumably originate from the contralateral IAS innervates the superior paraolivary nucleus (SPON). Notice that areas bordering the SPON that correspond to the medial nucleus of the trapezoid body (MNTB) and the lateral superior olive (LSO) are largely devoid of labeled fibers. (D) Micrograph of the ventral portion of the IAS, as it approaches its ipsilateral SOC. The thick, calretinin-positive axons presumably belong to octopus cells. (E) Co-staining of calretinin-positive fibers (red) and the postsynaptic marker potassium-chloride cotransporter 2 (KCC2; green) indicates that the SPON is densely innervated by calretinin-positive fibers, which presumably belong to octopus cells. (F) Co-staining of calretinin and KCC2 also demonstrates large calretinin-immunolabeled calyx-like synaptic specializations, which presumably belong to octopus cells, surrounding the cell bodies of neurons of the ventral nucleus of the lateral lemniscus (VNLL). The arrowheads indicate calretinin-positive axons approaching the VNLL. Orientation arrows in D also apply to (A,B) . Orientation arrows in (C) also apply to (E) . Calibration bar in (D) also applies to (C) .

    Journal: Frontiers in Neural Circuits

    Article Title: Octopus Cells in the Posteroventral Cochlear Nucleus Provide the Main Excitatory Input to the Superior Paraolivary Nucleus

    doi: 10.3389/fncir.2017.00037

    Figure Lengend Snippet: Immunolabeling of calretinin reveals the trajectory of the intermediate acoustic stria (IAS) from the posteroventral cochlear nucleus (PVCN) to the contralateral superior olivary complex. (A) Micrograph depicting the cell bodies of octopus cells densely immunolabeled for calretinin (asterisks). (B) The cell bodies of neurons of the anteroventral cochlear nucleus (AVCN) are immunonegative for calretinin and are surrounded by abundant calretinin-positive terminals. (C) A dense plexus of calretinin-positive fibers that presumably originate from the contralateral IAS innervates the superior paraolivary nucleus (SPON). Notice that areas bordering the SPON that correspond to the medial nucleus of the trapezoid body (MNTB) and the lateral superior olive (LSO) are largely devoid of labeled fibers. (D) Micrograph of the ventral portion of the IAS, as it approaches its ipsilateral SOC. The thick, calretinin-positive axons presumably belong to octopus cells. (E) Co-staining of calretinin-positive fibers (red) and the postsynaptic marker potassium-chloride cotransporter 2 (KCC2; green) indicates that the SPON is densely innervated by calretinin-positive fibers, which presumably belong to octopus cells. (F) Co-staining of calretinin and KCC2 also demonstrates large calretinin-immunolabeled calyx-like synaptic specializations, which presumably belong to octopus cells, surrounding the cell bodies of neurons of the ventral nucleus of the lateral lemniscus (VNLL). The arrowheads indicate calretinin-positive axons approaching the VNLL. Orientation arrows in D also apply to (A,B) . Orientation arrows in (C) also apply to (E) . Calibration bar in (D) also applies to (C) .

    Article Snippet: Sections were then incubated overnight at 4°C with the primary antibodies [goat anti-calretinin (AB1550; 1:500; Millipore, Solna, Sweden) and rabbit anti-KCC2 (potassium chloride cotransporter 2) (ANT-072; 1:200; Alomone Labs, Jerusalem, Israel)] diluted in a blocking solution that contained 2% normal donkey serum.

    Techniques: Immunolabeling, Labeling, Staining, Marker