antibodies anti tpc1  (Alomone Labs)


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    Name:
    Anti TPCN1 Antibody
    Description:
    Anti TPCN1 Antibody ACC 071 is a highly specific antibody directed against an epitope of the rat Two pore calcium channel protein 1 The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize TPCN1 from rat mouse and human samples
    Catalog Number:
    ACC-071
    Price:
    495.0
    Category:
    Primary Antibody
    Applications:
    Immunohistochemistry, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs antibodies anti tpc1
    Anti TPCN1 Antibody
    Anti TPCN1 Antibody ACC 071 is a highly specific antibody directed against an epitope of the rat Two pore calcium channel protein 1 The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize TPCN1 from rat mouse and human samples
    https://www.bioz.com/result/antibodies anti tpc1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies anti tpc1 - by Bioz Stars, 2021-09
    92/100 stars

    Images

    1) Product Images from "The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction"

    Article Title: The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction

    Journal: Cells

    doi: 10.3390/cells8101144

    Decrease in [Ca 2+ ] i rise in response to NE in SMCs transfected with siRNA against TPC1. ( A ) Kinetics of [Ca 2+ ] i rise in SMCs transfected with siRNA against TPC1 and nontarget siRNA. The curves from one of four transfection experiments are presented. Each point at the curves is an average of six parallel measurements. ( B ) Calcium responses to NE (100 μM) and angiotensin II (0.1 μM) in SMCs transfected with nontarget siRNA, and siRNA against TPC1 and against TPC2. [Ca 2+ ] i rise in SMCs transfected with nontarget siRNA is taken as 100%. The mean values + SEM from four independent transfection experiments with different SMCs preparations are presented. There is significant difference between the responses of SMCs transfected with anti-TPC1 siRNA and with nontarget (** p
    Figure Legend Snippet: Decrease in [Ca 2+ ] i rise in response to NE in SMCs transfected with siRNA against TPC1. ( A ) Kinetics of [Ca 2+ ] i rise in SMCs transfected with siRNA against TPC1 and nontarget siRNA. The curves from one of four transfection experiments are presented. Each point at the curves is an average of six parallel measurements. ( B ) Calcium responses to NE (100 μM) and angiotensin II (0.1 μM) in SMCs transfected with nontarget siRNA, and siRNA against TPC1 and against TPC2. [Ca 2+ ] i rise in SMCs transfected with nontarget siRNA is taken as 100%. The mean values + SEM from four independent transfection experiments with different SMCs preparations are presented. There is significant difference between the responses of SMCs transfected with anti-TPC1 siRNA and with nontarget (** p

    Techniques Used: Transfection

    Levels of TPC1 and TPC2 mRNA relative to β-actin mRNA ( A ). Western-blot of TPC1 and TPC2 proteins ( B ) isolated from rat aorta SMCs and relative levels of TPC1 and TPC2 ( C ). The values panel A are the means of four measurements in different SMCs preparation + SEM (* p
    Figure Legend Snippet: Levels of TPC1 and TPC2 mRNA relative to β-actin mRNA ( A ). Western-blot of TPC1 and TPC2 proteins ( B ) isolated from rat aorta SMCs and relative levels of TPC1 and TPC2 ( C ). The values panel A are the means of four measurements in different SMCs preparation + SEM (* p

    Techniques Used: Western Blot, Isolation

    2) Product Images from "The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction"

    Article Title: The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction

    Journal: Cells

    doi: 10.3390/cells8101144

    Decrease in [Ca 2+ ] i rise in response to NE in SMCs transfected with siRNA against TPC1. ( A ) Kinetics of [Ca 2+ ] i rise in SMCs transfected with siRNA against TPC1 and nontarget siRNA. The curves from one of four transfection experiments are presented. Each point at the curves is an average of six parallel measurements. ( B ) Calcium responses to NE (100 μM) and angiotensin II (0.1 μM) in SMCs transfected with nontarget siRNA, and siRNA against TPC1 and against TPC2. [Ca 2+ ] i rise in SMCs transfected with nontarget siRNA is taken as 100%. The mean values + SEM from four independent transfection experiments with different SMCs preparations are presented. There is significant difference between the responses of SMCs transfected with anti-TPC1 siRNA and with nontarget (** p
    Figure Legend Snippet: Decrease in [Ca 2+ ] i rise in response to NE in SMCs transfected with siRNA against TPC1. ( A ) Kinetics of [Ca 2+ ] i rise in SMCs transfected with siRNA against TPC1 and nontarget siRNA. The curves from one of four transfection experiments are presented. Each point at the curves is an average of six parallel measurements. ( B ) Calcium responses to NE (100 μM) and angiotensin II (0.1 μM) in SMCs transfected with nontarget siRNA, and siRNA against TPC1 and against TPC2. [Ca 2+ ] i rise in SMCs transfected with nontarget siRNA is taken as 100%. The mean values + SEM from four independent transfection experiments with different SMCs preparations are presented. There is significant difference between the responses of SMCs transfected with anti-TPC1 siRNA and with nontarget (** p

    Techniques Used: Transfection

    Levels of TPC1 and TPC2 mRNA relative to β-actin mRNA ( A ). Western-blot of TPC1 and TPC2 proteins ( B ) isolated from rat aorta SMCs and relative levels of TPC1 and TPC2 ( C ). The values panel A are the means of four measurements in different SMCs preparation + SEM (* p
    Figure Legend Snippet: Levels of TPC1 and TPC2 mRNA relative to β-actin mRNA ( A ). Western-blot of TPC1 and TPC2 proteins ( B ) isolated from rat aorta SMCs and relative levels of TPC1 and TPC2 ( C ). The values panel A are the means of four measurements in different SMCs preparation + SEM (* p

    Techniques Used: Western Blot, Isolation

    Related Articles

    Whole Genome Amplification:

    Article Title: The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction
    Article Snippet: .. Reagents cis -NED 19, trans-NED 19, U73122, and U73343 were acquired from Tocris (Bristol, UK); Fura2/AM, CalciumGreen/AM, and LysoTracker Red DND-99 from ThermoFischer Scientific (Waltham, MA, USA); Mitotracker Deep Red (M22426, Invitrogen), ER-tracker Red (E34250, Invitrogen), WGA Alexa Fluor 594 (Thermo Fisher, W11262), and antibodies anti-TPC1 (#ACC-071) and anti-TPC2 (#ACC-072) from Alomone Labs (Jerusalem, Israel); HRP-conjugated goat anti-rabbit IgG antibodies from BioRad (#170-6515); and the agonists of the receptors from SigmaAldrich (St. Louis, MO, USA). ..

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  • News
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  • Team
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  • 92
    Alomone Labs antibodies anti tpc1
    Decrease in [Ca 2+ ] i rise in response to NE in SMCs transfected with siRNA against <t>TPC1.</t> ( A ) Kinetics of [Ca 2+ ] i rise in SMCs transfected with siRNA against TPC1 and nontarget siRNA. The curves from one of four transfection experiments are presented. Each point at the curves is an average of six parallel measurements. ( B ) Calcium responses to NE (100 μM) and angiotensin II (0.1 μM) in SMCs transfected with nontarget siRNA, and siRNA against TPC1 and against TPC2. [Ca 2+ ] i rise in SMCs transfected with nontarget siRNA is taken as 100%. The mean values + SEM from four independent transfection experiments with different SMCs preparations are presented. There is significant difference between the responses of SMCs transfected with anti-TPC1 siRNA and with nontarget (** p
    Antibodies Anti Tpc1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies anti tpc1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies anti tpc1 - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Decrease in [Ca 2+ ] i rise in response to NE in SMCs transfected with siRNA against TPC1. ( A ) Kinetics of [Ca 2+ ] i rise in SMCs transfected with siRNA against TPC1 and nontarget siRNA. The curves from one of four transfection experiments are presented. Each point at the curves is an average of six parallel measurements. ( B ) Calcium responses to NE (100 μM) and angiotensin II (0.1 μM) in SMCs transfected with nontarget siRNA, and siRNA against TPC1 and against TPC2. [Ca 2+ ] i rise in SMCs transfected with nontarget siRNA is taken as 100%. The mean values + SEM from four independent transfection experiments with different SMCs preparations are presented. There is significant difference between the responses of SMCs transfected with anti-TPC1 siRNA and with nontarget (** p

    Journal: Cells

    Article Title: The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction

    doi: 10.3390/cells8101144

    Figure Lengend Snippet: Decrease in [Ca 2+ ] i rise in response to NE in SMCs transfected with siRNA against TPC1. ( A ) Kinetics of [Ca 2+ ] i rise in SMCs transfected with siRNA against TPC1 and nontarget siRNA. The curves from one of four transfection experiments are presented. Each point at the curves is an average of six parallel measurements. ( B ) Calcium responses to NE (100 μM) and angiotensin II (0.1 μM) in SMCs transfected with nontarget siRNA, and siRNA against TPC1 and against TPC2. [Ca 2+ ] i rise in SMCs transfected with nontarget siRNA is taken as 100%. The mean values + SEM from four independent transfection experiments with different SMCs preparations are presented. There is significant difference between the responses of SMCs transfected with anti-TPC1 siRNA and with nontarget (** p

    Article Snippet: Reagents cis -NED 19, trans-NED 19, U73122, and U73343 were acquired from Tocris (Bristol, UK); Fura2/AM, CalciumGreen/AM, and LysoTracker Red DND-99 from ThermoFischer Scientific (Waltham, MA, USA); Mitotracker Deep Red (M22426, Invitrogen), ER-tracker Red (E34250, Invitrogen), WGA Alexa Fluor 594 (Thermo Fisher, W11262), and antibodies anti-TPC1 (#ACC-071) and anti-TPC2 (#ACC-072) from Alomone Labs (Jerusalem, Israel); HRP-conjugated goat anti-rabbit IgG antibodies from BioRad (#170-6515); and the agonists of the receptors from SigmaAldrich (St. Louis, MO, USA).

    Techniques: Transfection

    Levels of TPC1 and TPC2 mRNA relative to β-actin mRNA ( A ). Western-blot of TPC1 and TPC2 proteins ( B ) isolated from rat aorta SMCs and relative levels of TPC1 and TPC2 ( C ). The values panel A are the means of four measurements in different SMCs preparation + SEM (* p

    Journal: Cells

    Article Title: The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction

    doi: 10.3390/cells8101144

    Figure Lengend Snippet: Levels of TPC1 and TPC2 mRNA relative to β-actin mRNA ( A ). Western-blot of TPC1 and TPC2 proteins ( B ) isolated from rat aorta SMCs and relative levels of TPC1 and TPC2 ( C ). The values panel A are the means of four measurements in different SMCs preparation + SEM (* p

    Article Snippet: Reagents cis -NED 19, trans-NED 19, U73122, and U73343 were acquired from Tocris (Bristol, UK); Fura2/AM, CalciumGreen/AM, and LysoTracker Red DND-99 from ThermoFischer Scientific (Waltham, MA, USA); Mitotracker Deep Red (M22426, Invitrogen), ER-tracker Red (E34250, Invitrogen), WGA Alexa Fluor 594 (Thermo Fisher, W11262), and antibodies anti-TPC1 (#ACC-071) and anti-TPC2 (#ACC-072) from Alomone Labs (Jerusalem, Israel); HRP-conjugated goat anti-rabbit IgG antibodies from BioRad (#170-6515); and the agonists of the receptors from SigmaAldrich (St. Louis, MO, USA).

    Techniques: Western Blot, Isolation