stim1  (Alomone Labs)


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    Alomone Labs stim1
    Increased TRPC1 and <t>STIM1</t> in MetS are abolished by exercise. ( A ) Representative agarose gel image quantifying TRPC1 mRNA using RT–PCR. ( B ) TRPC1 mRNA normalized to β-actin. STIM1 ( C ) and Orai1 ( D ) mRNA using quantitative RT–PCR
    Stim1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stim1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    stim1 - by Bioz Stars, 2022-05
    93/100 stars

    Images

    1) Product Images from "Exercise training decreases store-operated Ca2+entry associated with metabolic syndrome and coronary atherosclerosis"

    Article Title: Exercise training decreases store-operated Ca2+entry associated with metabolic syndrome and coronary atherosclerosis

    Journal: Cardiovascular Research

    doi: 10.1093/cvr/cvp308

    Increased TRPC1 and STIM1 in MetS are abolished by exercise. ( A ) Representative agarose gel image quantifying TRPC1 mRNA using RT–PCR. ( B ) TRPC1 mRNA normalized to β-actin. STIM1 ( C ) and Orai1 ( D ) mRNA using quantitative RT–PCR
    Figure Legend Snippet: Increased TRPC1 and STIM1 in MetS are abolished by exercise. ( A ) Representative agarose gel image quantifying TRPC1 mRNA using RT–PCR. ( B ) TRPC1 mRNA normalized to β-actin. STIM1 ( C ) and Orai1 ( D ) mRNA using quantitative RT–PCR

    Techniques Used: Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR

    2) Product Images from "Calcium Independent Effect of Orai1 and STIM1 in Non-Hodgkin B Cell Lymphoma Dissemination"

    Article Title: Calcium Independent Effect of Orai1 and STIM1 in Non-Hodgkin B Cell Lymphoma Dissemination

    Journal: Cancers

    doi: 10.3390/cancers10110402

    Stromal Derived Factor 1 (SDF-1) provokes an intracellular Ca 2+ response in the HLY-1 diffuse large B cell lymphoma (DLBCL) cell line involving intracellular Ca 2+ pool mobilization and Orai1/STIM1 extracellular Ca 2+ influx. Ca 2+ responses to SDF-1 (100 ng/mL) were measured using Fluo2-LR-AM Ca 2+ dye and recorded by videomicroscopy (Zeiss LSM 510) using ×25 objective. Black arrows indicate SDF-1 addition. Each trace represents the response of one cell and data are representative of at least three independent experiments. Typical response of unique cell (peak or peak follow by sustained plateau phase) are present as example ( Ab ). Data were processed using GraphPad prism. ( A ) Pharmacological characterization of SDF-1-induced Ca 2+ increase. Cells were recorded in extracellular saline solution (HBSS) containing 2 mM Ca 2+ (2 Ca, ( Aa )) or in Ca 2+ -free HBSS (0 Ca, ( Ac )). Cells were pre-incubated with BTP2 ( Ae ) or GSK7975A ( Af ) at 10 µM for 30 min and recorded in 2 mM Ca 2+ HBSS containing inhibitors. ( Ad , Ag ) Histograms represent areas under curves (AUC) calculated, under various recording conditions, between the application time of SDF-1 and t = 2050 s, and normalized compared to control (2 Ca or shNT). Data are expressed as mean ± SEM, * p
    Figure Legend Snippet: Stromal Derived Factor 1 (SDF-1) provokes an intracellular Ca 2+ response in the HLY-1 diffuse large B cell lymphoma (DLBCL) cell line involving intracellular Ca 2+ pool mobilization and Orai1/STIM1 extracellular Ca 2+ influx. Ca 2+ responses to SDF-1 (100 ng/mL) were measured using Fluo2-LR-AM Ca 2+ dye and recorded by videomicroscopy (Zeiss LSM 510) using ×25 objective. Black arrows indicate SDF-1 addition. Each trace represents the response of one cell and data are representative of at least three independent experiments. Typical response of unique cell (peak or peak follow by sustained plateau phase) are present as example ( Ab ). Data were processed using GraphPad prism. ( A ) Pharmacological characterization of SDF-1-induced Ca 2+ increase. Cells were recorded in extracellular saline solution (HBSS) containing 2 mM Ca 2+ (2 Ca, ( Aa )) or in Ca 2+ -free HBSS (0 Ca, ( Ac )). Cells were pre-incubated with BTP2 ( Ae ) or GSK7975A ( Af ) at 10 µM for 30 min and recorded in 2 mM Ca 2+ HBSS containing inhibitors. ( Ad , Ag ) Histograms represent areas under curves (AUC) calculated, under various recording conditions, between the application time of SDF-1 and t = 2050 s, and normalized compared to control (2 Ca or shNT). Data are expressed as mean ± SEM, * p

    Techniques Used: Derivative Assay, Incubation

    Orai1 and STIM1 regulate basal and SDF-1-induced DLBCL cell migration in a Ca 2+ independent manner in vitro. Cell migration was assessed in 96-transwell chemotaxis chambers assay. Histograms represent mean ± SEM from at least 3 independent experiments, * p
    Figure Legend Snippet: Orai1 and STIM1 regulate basal and SDF-1-induced DLBCL cell migration in a Ca 2+ independent manner in vitro. Cell migration was assessed in 96-transwell chemotaxis chambers assay. Histograms represent mean ± SEM from at least 3 independent experiments, * p

    Techniques Used: Migration, In Vitro, Chemotaxis Assay

    STIM1, but not Ca 2+ , regulate DLBCL dissemination in vivo. ( A ) Effect of STIM1 under-expression on HLY-1 cell dissemination. ( B ) Effect of intraperitoneal injection of BTP2 (12 µg/kg) or vehicle, three times per week on HLY-1 cell dissemination. Images were captured with a Nikon Eclipse Ci microscope equipped with a Plan Fluor 10× 0.3 NA objective. Scale bars = 150 μm. Histograms represent the quantification of positive surface for HLA-ABC staining. All tissues were delimited and to evaluate the percentage of positive surface for HLA-ABC staining on tissue, thresholding on positive and negative staining was done using Mercator software. Data are represented as mean ± SEM (n = 10), * p
    Figure Legend Snippet: STIM1, but not Ca 2+ , regulate DLBCL dissemination in vivo. ( A ) Effect of STIM1 under-expression on HLY-1 cell dissemination. ( B ) Effect of intraperitoneal injection of BTP2 (12 µg/kg) or vehicle, three times per week on HLY-1 cell dissemination. Images were captured with a Nikon Eclipse Ci microscope equipped with a Plan Fluor 10× 0.3 NA objective. Scale bars = 150 μm. Histograms represent the quantification of positive surface for HLA-ABC staining. All tissues were delimited and to evaluate the percentage of positive surface for HLA-ABC staining on tissue, thresholding on positive and negative staining was done using Mercator software. Data are represented as mean ± SEM (n = 10), * p

    Techniques Used: In Vivo, Expressing, Injection, Microscopy, Staining, Negative Staining, Software

    Orai1 and STIM1 control DLBCL cell migration through RhoA activation, ROCK, and MLC phosphorylation. ( A ) SDF-1-induced SU-DHL-4 and HLY-1 cell migration is ROCK activation dependent. Cells incubated or not with SDF-1 (100 ng/mL) were pre-treated during 20 min in the presence or not of various inhibitors (FAK inhibitor 1 µM, PD98059 a MEK inhibitor 10 µM, Wortmannin a PI3K inhibitor 10 nM, AKT inhibitor 250 nM, Y27632 a ROCK inhibitor 1 µM). Data are represented as mean ± SEM of 3 independent experiments, * p
    Figure Legend Snippet: Orai1 and STIM1 control DLBCL cell migration through RhoA activation, ROCK, and MLC phosphorylation. ( A ) SDF-1-induced SU-DHL-4 and HLY-1 cell migration is ROCK activation dependent. Cells incubated or not with SDF-1 (100 ng/mL) were pre-treated during 20 min in the presence or not of various inhibitors (FAK inhibitor 1 µM, PD98059 a MEK inhibitor 10 µM, Wortmannin a PI3K inhibitor 10 nM, AKT inhibitor 250 nM, Y27632 a ROCK inhibitor 1 µM). Data are represented as mean ± SEM of 3 independent experiments, * p

    Techniques Used: Migration, Activation Assay, Incubation

    Orai1 and STIM1 expression is altered in extra-nodal DLBCL. ( A ) Representative immunofluorescent staining of STIM1 and Orai1 in normal lymph node and extra-nodal DLBCL under-expressing STIM1 or Orai1. Images were acquired using Leica DMI8 microscope equipped with a 40× oil immersion objective. TMA including samples from normal lymph node (n = 26), nodal (n = 43) and extra-nodal (n = 44) DLBCL was co-immunostained using Orai1 or STIM1 antibodies revealed by donkey anti-rabbit Alexa-488 (in green) and mouse anti-human CD20 and anti-human CD19 revealed by goat anti-mouse Alexa 532 (in red). Nuclei were stained with DAPI (in blue). Scale bar = 50 µm. ( B ) Quantification of STIM1 or Orai1 alteration in DLBCL samples. After acquisition of spot fluorescence on Icys laser scanning cytometer, a segmentation analysis base on phantoms was done to determine the percentage of CD20/CD19 positive cells expressing protein of interest for each spot. The stacked column charts illustrate the intensity grade for STIM1 or Orai1 expression in surgical specimens. * p
    Figure Legend Snippet: Orai1 and STIM1 expression is altered in extra-nodal DLBCL. ( A ) Representative immunofluorescent staining of STIM1 and Orai1 in normal lymph node and extra-nodal DLBCL under-expressing STIM1 or Orai1. Images were acquired using Leica DMI8 microscope equipped with a 40× oil immersion objective. TMA including samples from normal lymph node (n = 26), nodal (n = 43) and extra-nodal (n = 44) DLBCL was co-immunostained using Orai1 or STIM1 antibodies revealed by donkey anti-rabbit Alexa-488 (in green) and mouse anti-human CD20 and anti-human CD19 revealed by goat anti-mouse Alexa 532 (in red). Nuclei were stained with DAPI (in blue). Scale bar = 50 µm. ( B ) Quantification of STIM1 or Orai1 alteration in DLBCL samples. After acquisition of spot fluorescence on Icys laser scanning cytometer, a segmentation analysis base on phantoms was done to determine the percentage of CD20/CD19 positive cells expressing protein of interest for each spot. The stacked column charts illustrate the intensity grade for STIM1 or Orai1 expression in surgical specimens. * p

    Techniques Used: Expressing, Staining, Microscopy, Fluorescence, Cytometry

    3) Product Images from "Mast Cell CRF2 Suppresses Mast Cell Degranulation and Limits the Severity of Anaphylaxis and Stress-Induced Intestinal Permeability"

    Article Title: Mast Cell CRF2 Suppresses Mast Cell Degranulation and Limits the Severity of Anaphylaxis and Stress-Induced Intestinal Permeability

    Journal: The Journal of allergy and clinical immunology

    doi: 10.1016/j.jaci.2018.08.053

    CRF2−/− BMMCs exhibit heightened intracellular Ca 2+ store release and expression of SOCE channels. BMMCs derived from WT and CRF 2 −/− mice were loaded with Fluo 4, and intracellular Ca 2+ levels were measured following stimulation with IgE/DNP. A,B: Representative intracellular Ca 2+ traces for experiments conducted under Ca 2+ -replete (A) or Ca 2+ -free (1 mM EDTA; B) conditions. C: Mean peak change in fluorescence following IgE/DNP stimulus presented as ∆ peak fluorescence. D-I: Representative Western blots and densitometry analysis for STIM1 (D, G), TRPC1 (E, H), and Orai (F, I) in WT and CRF 2 −/− BMMCs. *Significance between groups was determined by an unpaired two-tailed t-test (C, G-I),*p
    Figure Legend Snippet: CRF2−/− BMMCs exhibit heightened intracellular Ca 2+ store release and expression of SOCE channels. BMMCs derived from WT and CRF 2 −/− mice were loaded with Fluo 4, and intracellular Ca 2+ levels were measured following stimulation with IgE/DNP. A,B: Representative intracellular Ca 2+ traces for experiments conducted under Ca 2+ -replete (A) or Ca 2+ -free (1 mM EDTA; B) conditions. C: Mean peak change in fluorescence following IgE/DNP stimulus presented as ∆ peak fluorescence. D-I: Representative Western blots and densitometry analysis for STIM1 (D, G), TRPC1 (E, H), and Orai (F, I) in WT and CRF 2 −/− BMMCs. *Significance between groups was determined by an unpaired two-tailed t-test (C, G-I),*p

    Techniques Used: Expressing, Derivative Assay, Mouse Assay, Fluorescence, Western Blot, Two Tailed Test

    4) Product Images from "Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells"

    Article Title: Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells

    Journal: Cancers

    doi: 10.3390/cancers11010083

    Effects of DFMO on the expression of proteins involved in SOCE in colon cancer HT29 cells. HT29 cells were treated with vehicle (control) or DFMO 5 mM, and then cells were lysed and subjected to Western blotting with antibodies against TRPC1, STIM1, STIM2, ORAI1, ORAI2 and ORAI3, followed by reprobing with anti-β-actin antibody for protein loading control. Bar graphs represent specific protein expression normalized to the β-actin content. Data are from n = 3 experiments (* p
    Figure Legend Snippet: Effects of DFMO on the expression of proteins involved in SOCE in colon cancer HT29 cells. HT29 cells were treated with vehicle (control) or DFMO 5 mM, and then cells were lysed and subjected to Western blotting with antibodies against TRPC1, STIM1, STIM2, ORAI1, ORAI2 and ORAI3, followed by reprobing with anti-β-actin antibody for protein loading control. Bar graphs represent specific protein expression normalized to the β-actin content. Data are from n = 3 experiments (* p

    Techniques Used: Expressing, Western Blot

    Effects of DFMO on the expression of genes coding for SOCE molecular players in HT29 cells. mRNA expression levels of selected genes were determined using qRT-PCR of extracts from control and DFMO-treated HT29 cells. β-actin was used as a reference. Data results are mean ± SEM from DFMO-treated cells relative to untreated cells Data are from n = 7, 7, 6, 6, 6, and 5 experiments for TRPC1 , STIM1 , STIM2 , ORAI1 , ORAI2 , and ORAI3 , respectively * p
    Figure Legend Snippet: Effects of DFMO on the expression of genes coding for SOCE molecular players in HT29 cells. mRNA expression levels of selected genes were determined using qRT-PCR of extracts from control and DFMO-treated HT29 cells. β-actin was used as a reference. Data results are mean ± SEM from DFMO-treated cells relative to untreated cells Data are from n = 7, 7, 6, 6, 6, and 5 experiments for TRPC1 , STIM1 , STIM2 , ORAI1 , ORAI2 , and ORAI3 , respectively * p

    Techniques Used: Expressing, Quantitative RT-PCR

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    Alomone Labs stim1
    Increased TRPC1 and <t>STIM1</t> in MetS are abolished by exercise. ( A ) Representative agarose gel image quantifying TRPC1 mRNA using RT–PCR. ( B ) TRPC1 mRNA normalized to β-actin. STIM1 ( C ) and Orai1 ( D ) mRNA using quantitative RT–PCR
    Stim1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stim1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    stim1 - by Bioz Stars, 2022-05
    93/100 stars
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    Increased TRPC1 and STIM1 in MetS are abolished by exercise. ( A ) Representative agarose gel image quantifying TRPC1 mRNA using RT–PCR. ( B ) TRPC1 mRNA normalized to β-actin. STIM1 ( C ) and Orai1 ( D ) mRNA using quantitative RT–PCR

    Journal: Cardiovascular Research

    Article Title: Exercise training decreases store-operated Ca2+entry associated with metabolic syndrome and coronary atherosclerosis

    doi: 10.1093/cvr/cvp308

    Figure Lengend Snippet: Increased TRPC1 and STIM1 in MetS are abolished by exercise. ( A ) Representative agarose gel image quantifying TRPC1 mRNA using RT–PCR. ( B ) TRPC1 mRNA normalized to β-actin. STIM1 ( C ) and Orai1 ( D ) mRNA using quantitative RT–PCR

    Article Snippet: STIM1 and ORAI1 antibodies were from Alomone labs (Rehovet, Israel).

    Techniques: Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR