kcnmb4  (Alomone Labs)


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    Alomone Labs kcnmb4
    BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of <t>KCNMB4</t> in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.
    Kcnmb4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kcnmb4/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kcnmb4 - by Bioz Stars, 2022-05
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    1) Product Images from "Big conductance calcium‐activated potassium channel openers control spasticity without sedation) Big conductance calcium‐activated potassium channel openers control spasticity without sedation"

    Article Title: Big conductance calcium‐activated potassium channel openers control spasticity without sedation) Big conductance calcium‐activated potassium channel openers control spasticity without sedation

    Journal: British Journal of Pharmacology

    doi: 10.1111/bph.13889

    BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of KCNMB4 in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.
    Figure Legend Snippet: BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of KCNMB4 in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.

    Techniques Used: Expressing, Western Blot, Mouse Assay, Injection

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    Alomone Labs kcnmb4
    BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of <t>KCNMB4</t> in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.
    Kcnmb4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kcnmb4/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kcnmb4 - by Bioz Stars, 2022-05
    91/100 stars
      Buy from Supplier

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    Alomone Labs orai2
    Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between <t>ORAI2</t> and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value
    Orai2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orai2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orai2 - by Bioz Stars, 2022-05
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    BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of KCNMB4 in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.

    Journal: British Journal of Pharmacology

    Article Title: Big conductance calcium‐activated potassium channel openers control spasticity without sedation) Big conductance calcium‐activated potassium channel openers control spasticity without sedation

    doi: 10.1111/bph.13889

    Figure Lengend Snippet: BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of KCNMB4 in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.

    Article Snippet: Membranes were washed, blocked for 1 h in blocking buffer (5% non‐fat dried milk) and incubated in blocking buffer with primary antibody using rabbit polyclonal antibodies against mouse/human KCNMA1 (APC‐021, previously validated by lack of activity in big conductance calcium‐activated potassium channel Kcnma1 ‐deficient mice and APC‐151 antibody) and KCNMB4 (APC‐061 antibody, previously validated by lack of activity in Kcnmb4‐deficient mice despite detecting multiple isoforms), which were purchased from Alomone Labs, Jerusalem Israel, whose website reports supporting literature concerning characterization of the antibodies.

    Techniques: Expressing, Western Blot, Mouse Assay, Injection

    Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between ORAI2 and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value

    Journal: PLoS ONE

    Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes

    doi: 10.1371/journal.pone.0208981

    Figure Lengend Snippet: Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between ORAI2 and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value

    Article Snippet: The membranes were blocked with 10% FBS in Tris buffered saline containing 0.1% Tween (TBS-T) for 1 h and incubated overnight at 4°C with primary antibodies against STIM2 (1:200, Cell Signaling Technology, Cat No. 4917), ORAI1 (1:500, Alomone labs, Cat No. ACC-060), ORAI2 (1:500, Alomone labs, Cat No. ACC-061), CaV 1.3 (1:500, Alomone labs, Cat No. ACC-005), CaV 2.3 (1:500, Alomone labs, Cat No. ACC-006) and GAPDH (1:3000; Merck Millipore, Cat No. ABS16).

    Techniques: Expressing

    Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-pregnant controls and pregnant women. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Ca V 1.1 subunit mRNA was not detected in any sample. Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: ORAI1, df = 48, p = 0.003; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI2, H (1, 46) = 28.5, p

    Journal: PLoS ONE

    Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes

    doi: 10.1371/journal.pone.0208981

    Figure Lengend Snippet: Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-pregnant controls and pregnant women. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Ca V 1.1 subunit mRNA was not detected in any sample. Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: ORAI1, df = 48, p = 0.003; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI2, H (1, 46) = 28.5, p

    Article Snippet: The membranes were blocked with 10% FBS in Tris buffered saline containing 0.1% Tween (TBS-T) for 1 h and incubated overnight at 4°C with primary antibodies against STIM2 (1:200, Cell Signaling Technology, Cat No. 4917), ORAI1 (1:500, Alomone labs, Cat No. ACC-060), ORAI2 (1:500, Alomone labs, Cat No. ACC-061), CaV 1.3 (1:500, Alomone labs, Cat No. ACC-005), CaV 2.3 (1:500, Alomone labs, Cat No. ACC-006) and GAPDH (1:3000; Merck Millipore, Cat No. ABS16).

    Techniques: Expressing