anti trpm6 antibody  (Alomone Labs)


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    Name:
    Anti TRPM6 extracellular Antibody
    Description:
    Anti TRPM6 extracellular Antibody ACC 046 is a highly specific antibody directed against an epitope of the mouse protein The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize TRPM6 from mouse rat and human samples
    Catalog Number:
    ACC-046
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunohistochemistry, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs anti trpm6 antibody
    Anti TRPM6 extracellular Antibody
    Anti TRPM6 extracellular Antibody ACC 046 is a highly specific antibody directed against an epitope of the mouse protein The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize TRPM6 from mouse rat and human samples
    https://www.bioz.com/result/anti trpm6 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpm6 antibody - by Bioz Stars, 2021-10
    94/100 stars

    Images

    1) Product Images from "Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry"

    Article Title: Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-21004-w

    TRPM7/TRPM6 protein expression and TRPM7 kinase activity in splenic T cells. ( A ) Western blot analysis of immunoprecipitated TRPM7 from whole cell lysates of WT and KD splenic T cells. T cells were stimulated with PMA/ionomycin or anti-CD3/CD28 antibody coated beads for 48 hrs. Mouse embryonic fibroblasts were used as a positive control. Equal amounts of protein before immunoprecipitation were ensured by probing for actin. ( B ) Incorporation of 32 P into exogenous myelin basic protein (MBP) by TRPM7 immunoprecipitated from WT and KD resting T cells. Equal quantities of MBP were verified by coomassie blue staining. ( C ) Control experiment showing that anti-TRPM6 antibody was able to recognize TRPM6, by immunoprecipitation using anti-TRPM6 antibody in GFP-TRPM6 transfected HEK cells ( D .
    Figure Legend Snippet: TRPM7/TRPM6 protein expression and TRPM7 kinase activity in splenic T cells. ( A ) Western blot analysis of immunoprecipitated TRPM7 from whole cell lysates of WT and KD splenic T cells. T cells were stimulated with PMA/ionomycin or anti-CD3/CD28 antibody coated beads for 48 hrs. Mouse embryonic fibroblasts were used as a positive control. Equal amounts of protein before immunoprecipitation were ensured by probing for actin. ( B ) Incorporation of 32 P into exogenous myelin basic protein (MBP) by TRPM7 immunoprecipitated from WT and KD resting T cells. Equal quantities of MBP were verified by coomassie blue staining. ( C ) Control experiment showing that anti-TRPM6 antibody was able to recognize TRPM6, by immunoprecipitation using anti-TRPM6 antibody in GFP-TRPM6 transfected HEK cells ( D .

    Techniques Used: Expressing, Activity Assay, Western Blot, Immunoprecipitation, Positive Control, Staining, Transfection

    2) Product Images from "The role of calbindin-D28k on renal calcium and magnesium handling during treatment with loop and thiazide diuretics"

    Article Title: The role of calbindin-D28k on renal calcium and magnesium handling during treatment with loop and thiazide diuretics

    Journal: American Journal of Physiology - Renal Physiology

    doi: 10.1152/ajprenal.00057.2015

    Immunofluorescence staining of CBD-28k ( A ), TRPV5 ( B ), and TRPM6 ( C ) in renal tissue of wild-type and CBD-28k KO mice following CTZ and FSM treatment.
    Figure Legend Snippet: Immunofluorescence staining of CBD-28k ( A ), TRPV5 ( B ), and TRPM6 ( C ) in renal tissue of wild-type and CBD-28k KO mice following CTZ and FSM treatment.

    Techniques Used: Immunofluorescence, Staining, Mouse Assay

    Immunoblotting study of CBD-28k ( A ) TRPV5 ( B ), TRPM6 ( C ), and claudin-16 ( D ) in renal tissue of wild-type and CBD-28k KO mice following CTZ and FSM treatment. The whole blots are shown with molecular size markers labeled on the left . The specific bands
    Figure Legend Snippet: Immunoblotting study of CBD-28k ( A ) TRPV5 ( B ), TRPM6 ( C ), and claudin-16 ( D ) in renal tissue of wild-type and CBD-28k KO mice following CTZ and FSM treatment. The whole blots are shown with molecular size markers labeled on the left . The specific bands

    Techniques Used: Mouse Assay, Labeling

    3) Product Images from "Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart"

    Article Title: Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-94856-4

    Effect on incubating human cardiomyocytes in acidic solution on the immunofluorescence of TRPM6 and TPRM7. ( a, b ) Quantification of the intensity of fluorescence at pH = 7.4 ( open symbols ) and at pH = 5.0 ( filled symbols ) expressed in arbitrary units (a.u.) with and without divalent cations, respectively. * P
    Figure Legend Snippet: Effect on incubating human cardiomyocytes in acidic solution on the immunofluorescence of TRPM6 and TPRM7. ( a, b ) Quantification of the intensity of fluorescence at pH = 7.4 ( open symbols ) and at pH = 5.0 ( filled symbols ) expressed in arbitrary units (a.u.) with and without divalent cations, respectively. * P

    Techniques Used: Immunofluorescence, Fluorescence

    Immunofluorescence images depicting co-expression of TRPM6 and TRPM7 proteins in human cardiomyocytes. ( a ) The immunofluorescence of TRPM7 ( green ) and TRPM6 ( red ) in the same LA, RA, LV, and RV cardiomyocyte when using conjugated antibodies (the arrowheads indicate the localization of TRPM6 protein in perinuclear area). ( b ) Quantification of the staining intensity of the immunodetected conjugated antibodies ( spotty ) and non-conjugated antibodies ( smooth ) for both proteins in cardiomyocytes from the four chambers of the heart as indicated. * P
    Figure Legend Snippet: Immunofluorescence images depicting co-expression of TRPM6 and TRPM7 proteins in human cardiomyocytes. ( a ) The immunofluorescence of TRPM7 ( green ) and TRPM6 ( red ) in the same LA, RA, LV, and RV cardiomyocyte when using conjugated antibodies (the arrowheads indicate the localization of TRPM6 protein in perinuclear area). ( b ) Quantification of the staining intensity of the immunodetected conjugated antibodies ( spotty ) and non-conjugated antibodies ( smooth ) for both proteins in cardiomyocytes from the four chambers of the heart as indicated. * P

    Techniques Used: Immunofluorescence, Expressing, Staining

    Representative histotopograms of TRPM6 images in the four chamber walls of the human heart. ( a ) Control subject after traffic accident. ( b ) IHD patient. The intensity of the brownish pigments shows areas with expression of TRPM6 in the heart (× 40 magnification). ( c ) Negative control ( left ) in the LV, and positive control ( right ) of TRPM6 in tumour of the colon (× 40 magnification). Scale bars indicate 100 µm. Other notations are the same as in Fig. 7 .
    Figure Legend Snippet: Representative histotopograms of TRPM6 images in the four chamber walls of the human heart. ( a ) Control subject after traffic accident. ( b ) IHD patient. The intensity of the brownish pigments shows areas with expression of TRPM6 in the heart (× 40 magnification). ( c ) Negative control ( left ) in the LV, and positive control ( right ) of TRPM6 in tumour of the colon (× 40 magnification). Scale bars indicate 100 µm. Other notations are the same as in Fig. 7 .

    Techniques Used: Expressing, Negative Control, Positive Control

    Effect of 2-APB and CAR on the immunofluorescence of TRPM6 and TPRM7 in human cardiomyocytes. ( a – f ): Cardiomyocyte staining with anti-TRPM7 ( a , c , e ) or anti-TRPM6 ( b , d , f ) in the absence ( a , b ) of drugs and in the presence of either 2-APB ( c , d ) or CAR ( e , f ). ( g – j ): Quantification of the intensity of fluorescence without drugs ( open ) and with the drugs ( filled ) expressed in arbitrary units (a.u.). Note lack of influence of the solvent, DMSO, at 500 μmol/L (triangles) but opposite change with 2-APB and CAR on TRPM7 vs. TRPM6, i.e. decrease of the immunofluorescence level of TRPM7 but increase of the TRPM6 fluorescence level. * P
    Figure Legend Snippet: Effect of 2-APB and CAR on the immunofluorescence of TRPM6 and TPRM7 in human cardiomyocytes. ( a – f ): Cardiomyocyte staining with anti-TRPM7 ( a , c , e ) or anti-TRPM6 ( b , d , f ) in the absence ( a , b ) of drugs and in the presence of either 2-APB ( c , d ) or CAR ( e , f ). ( g – j ): Quantification of the intensity of fluorescence without drugs ( open ) and with the drugs ( filled ) expressed in arbitrary units (a.u.). Note lack of influence of the solvent, DMSO, at 500 μmol/L (triangles) but opposite change with 2-APB and CAR on TRPM7 vs. TRPM6, i.e. decrease of the immunofluorescence level of TRPM7 but increase of the TRPM6 fluorescence level. * P

    Techniques Used: Immunofluorescence, Staining, Fluorescence

    Comparison of the levels of TRPM6 and TRPM7 in IHD vs. non-IHD. ( a–d , e–h ) Quantification of the intensity of fluorescence in cardiomyocytes obtained from patients with IHD ( filled symbols ) and without such diagnosis ( unfilled symbols ) expressed in arbitrary units (a.u.) in presence/absence of divalent cations, 2 h and 12 h, respectively. In all cells used P
    Figure Legend Snippet: Comparison of the levels of TRPM6 and TRPM7 in IHD vs. non-IHD. ( a–d , e–h ) Quantification of the intensity of fluorescence in cardiomyocytes obtained from patients with IHD ( filled symbols ) and without such diagnosis ( unfilled symbols ) expressed in arbitrary units (a.u.) in presence/absence of divalent cations, 2 h and 12 h, respectively. In all cells used P

    Techniques Used: Fluorescence

    Immunofluorescence of TRPM7 and TRPM6 proteins in all cells used. Image acquisition performed using confocal laser scanning microscope ( a , atria; b , ventricle). Immunofluorescence of confocal z-stack of cardiomyocytes with immunodetected TRPM7 and TRPM6 proteins, respectively. Alexa Fluor 488 and Alexa Fluor 546 for the TRPM7 and TRPM6 protein appear in green and red, respectively. Alexa Fluor 405 for F-actin cytoskeleton appears in surrogate grey. Hoechst 33342 for nuclei appears in blue (the arrowheads indicate the localization of TRPM6 protein in the perinuclear area). ( c , d ) Quantification of immunofluorescence levels of the TRPM7 ( green ) and TRPM6 ( red ) proteins in cardiomyocytes from four chambers of the heart (left atrium, LA; right atrium, RA; left ventricle, LV; and right ventricle, RV), under experimental conditions with ( c ) and without ( d ) divalent cations in the extracellular milieu, respectively. Cardiomyocytes were fixed following 2 h ( filled columns ) or 12 h ( unfilled columns ) after cell isolation. Mean data provided in arbitrary units (a.u.) (Supplementary Table 1 online). A blinded study-design (with the investigator reading the fluorescence not knowing the cell incubation conditions) was used for the detection of protein concentration during various experimental conditions. * P
    Figure Legend Snippet: Immunofluorescence of TRPM7 and TRPM6 proteins in all cells used. Image acquisition performed using confocal laser scanning microscope ( a , atria; b , ventricle). Immunofluorescence of confocal z-stack of cardiomyocytes with immunodetected TRPM7 and TRPM6 proteins, respectively. Alexa Fluor 488 and Alexa Fluor 546 for the TRPM7 and TRPM6 protein appear in green and red, respectively. Alexa Fluor 405 for F-actin cytoskeleton appears in surrogate grey. Hoechst 33342 for nuclei appears in blue (the arrowheads indicate the localization of TRPM6 protein in the perinuclear area). ( c , d ) Quantification of immunofluorescence levels of the TRPM7 ( green ) and TRPM6 ( red ) proteins in cardiomyocytes from four chambers of the heart (left atrium, LA; right atrium, RA; left ventricle, LV; and right ventricle, RV), under experimental conditions with ( c ) and without ( d ) divalent cations in the extracellular milieu, respectively. Cardiomyocytes were fixed following 2 h ( filled columns ) or 12 h ( unfilled columns ) after cell isolation. Mean data provided in arbitrary units (a.u.) (Supplementary Table 1 online). A blinded study-design (with the investigator reading the fluorescence not knowing the cell incubation conditions) was used for the detection of protein concentration during various experimental conditions. * P

    Techniques Used: Immunofluorescence, Laser-Scanning Microscopy, Cell Isolation, Fluorescence, Incubation, Protein Concentration

    TRPM7 and TRPM6 protein levels and RT-qPCR mRNA relative expression levels in human heart tissue homogenates. ( a, b ) TRPM7 and TRPM6 proteins are increased in the walls of all heart chambers with IHD ( filled columns ) vs. non-IHD ( unfilled columns ). A blinded study-design (with the diagnosis unknown to the investigator) was used for the detection of protein concentration in the various samples. Values (mean ± SEM) are in pg/mL and from 3–33 heart tissue homogenates. * P
    Figure Legend Snippet: TRPM7 and TRPM6 protein levels and RT-qPCR mRNA relative expression levels in human heart tissue homogenates. ( a, b ) TRPM7 and TRPM6 proteins are increased in the walls of all heart chambers with IHD ( filled columns ) vs. non-IHD ( unfilled columns ). A blinded study-design (with the diagnosis unknown to the investigator) was used for the detection of protein concentration in the various samples. Values (mean ± SEM) are in pg/mL and from 3–33 heart tissue homogenates. * P

    Techniques Used: Quantitative RT-PCR, Expressing, Protein Concentration

    Related Articles

    Binding Assay:

    Article Title: Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart
    Article Snippet: .. Non-specific binding of antibody was prevented by using blocking buffer containing 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 1 h. Cells were incubated with primary rabbit polyclonal anti-TRPM6 antibody (ACC-046, Alomone labs, Jerusalem, Israel) or rabbit polyclonal anti-TRPM7 antibody (ACC-047, Alomone labs, Jerusalem, Israel) diluted (1:200) in PBS containing 3% BSA in blocking buffer overnight at 4 °C, and washed thereafter with PBS. ..

    Blocking Assay:

    Article Title: Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart
    Article Snippet: .. Non-specific binding of antibody was prevented by using blocking buffer containing 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 1 h. Cells were incubated with primary rabbit polyclonal anti-TRPM6 antibody (ACC-046, Alomone labs, Jerusalem, Israel) or rabbit polyclonal anti-TRPM7 antibody (ACC-047, Alomone labs, Jerusalem, Israel) diluted (1:200) in PBS containing 3% BSA in blocking buffer overnight at 4 °C, and washed thereafter with PBS. ..

    Article Title: The role of calbindin-D28k on renal calcium and magnesium handling during treatment with loop and thiazide diuretics
    Article Snippet: .. The membrane was incubated with an anti-TRPV5 antibody (1:1,000, Abcam), anti-CBD-28k antibody (1:20,000, Swant, Marly, Switzerland), anti-TRPM6 antibody (1:1,000, Alomone Laboratory, Jerusalem, Israel), and anti-CLDN16 antibody (1:3,000, Abcam) after blocking with 10% nonfat milk. ..

    Incubation:

    Article Title: Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart
    Article Snippet: .. Non-specific binding of antibody was prevented by using blocking buffer containing 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 1 h. Cells were incubated with primary rabbit polyclonal anti-TRPM6 antibody (ACC-046, Alomone labs, Jerusalem, Israel) or rabbit polyclonal anti-TRPM7 antibody (ACC-047, Alomone labs, Jerusalem, Israel) diluted (1:200) in PBS containing 3% BSA in blocking buffer overnight at 4 °C, and washed thereafter with PBS. ..

    Article Title: Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry
    Article Snippet: .. Extracts of T cells, mouse embryonic fibroblasts (MEF) or HEK293 cells (untransfected or heterologously expressing GFP tagged human TRPM6 in pEGFP-C1 vector) were obtained as described above for the TRPM7 kinase assay and incubated with anti-TRPM7 antibody (1:500) or anti-TRPM6 antibody (1:500, ACC-046; Alomone labs, Israel) overnight at 4 °C, followed by incubation with protein A sepharose beads for 1 hr. ..

    Article Title: The role of calbindin-D28k on renal calcium and magnesium handling during treatment with loop and thiazide diuretics
    Article Snippet: .. The membrane was incubated with an anti-TRPV5 antibody (1:1,000, Abcam), anti-CBD-28k antibody (1:20,000, Swant, Marly, Switzerland), anti-TRPM6 antibody (1:1,000, Alomone Laboratory, Jerusalem, Israel), and anti-CLDN16 antibody (1:3,000, Abcam) after blocking with 10% nonfat milk. ..

    Expressing:

    Article Title: Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry
    Article Snippet: .. Extracts of T cells, mouse embryonic fibroblasts (MEF) or HEK293 cells (untransfected or heterologously expressing GFP tagged human TRPM6 in pEGFP-C1 vector) were obtained as described above for the TRPM7 kinase assay and incubated with anti-TRPM7 antibody (1:500) or anti-TRPM6 antibody (1:500, ACC-046; Alomone labs, Israel) overnight at 4 °C, followed by incubation with protein A sepharose beads for 1 hr. ..

    Plasmid Preparation:

    Article Title: Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry
    Article Snippet: .. Extracts of T cells, mouse embryonic fibroblasts (MEF) or HEK293 cells (untransfected or heterologously expressing GFP tagged human TRPM6 in pEGFP-C1 vector) were obtained as described above for the TRPM7 kinase assay and incubated with anti-TRPM7 antibody (1:500) or anti-TRPM6 antibody (1:500, ACC-046; Alomone labs, Israel) overnight at 4 °C, followed by incubation with protein A sepharose beads for 1 hr. ..

    Kinase Assay:

    Article Title: Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry
    Article Snippet: .. Extracts of T cells, mouse embryonic fibroblasts (MEF) or HEK293 cells (untransfected or heterologously expressing GFP tagged human TRPM6 in pEGFP-C1 vector) were obtained as described above for the TRPM7 kinase assay and incubated with anti-TRPM7 antibody (1:500) or anti-TRPM6 antibody (1:500, ACC-046; Alomone labs, Israel) overnight at 4 °C, followed by incubation with protein A sepharose beads for 1 hr. ..

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  • 94
    Alomone Labs anti trpm6 antibody
    <t>TRPM7/TRPM6</t> protein expression and TRPM7 kinase activity in splenic T cells. ( A ) Western blot analysis of immunoprecipitated TRPM7 from whole cell lysates of WT and KD splenic T cells. T cells were stimulated with PMA/ionomycin or anti-CD3/CD28 antibody coated beads for 48 hrs. Mouse embryonic fibroblasts were used as a positive control. Equal amounts of protein before immunoprecipitation were ensured by probing for actin. ( B ) Incorporation of 32 P into exogenous myelin basic protein (MBP) by TRPM7 immunoprecipitated from WT and KD resting T cells. Equal quantities of MBP were verified by coomassie blue staining. ( C ) Control experiment showing that anti-TRPM6 antibody was able to recognize TRPM6, by immunoprecipitation using anti-TRPM6 antibody in GFP-TRPM6 transfected HEK cells ( D .
    Anti Trpm6 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpm6 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpm6 antibody - by Bioz Stars, 2021-10
    94/100 stars
      Buy from Supplier

    86
    millipore anti trpm6 antibody
    <t>TRPM7/TRPM6</t> protein expression and TRPM7 kinase activity in splenic T cells. ( A ) Western blot analysis of immunoprecipitated TRPM7 from whole cell lysates of WT and KD splenic T cells. T cells were stimulated with PMA/ionomycin or anti-CD3/CD28 antibody coated beads for 48 hrs. Mouse embryonic fibroblasts were used as a positive control. Equal amounts of protein before immunoprecipitation were ensured by probing for actin. ( B ) Incorporation of 32 P into exogenous myelin basic protein (MBP) by TRPM7 immunoprecipitated from WT and KD resting T cells. Equal quantities of MBP were verified by coomassie blue staining. ( C ) Control experiment showing that anti-TRPM6 antibody was able to recognize TRPM6, by immunoprecipitation using anti-TRPM6 antibody in GFP-TRPM6 transfected HEK cells ( D ). Western blot analysis of TRPM6 immunoprecipitated from WT and KD mouse T cells and kidneys. Full gel images are provided in Supplementary Fig. S6 .
    Anti Trpm6 Antibody, supplied by millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpm6 antibody/product/millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpm6 antibody - by Bioz Stars, 2021-10
    86/100 stars
      Buy from Supplier

    80
    Alomone Labs anti trpm6 extracellular antibody
    Immunofluorescence images suggesting the presence of <t>TRPM6</t> and TRPM7 proteins in pig cardiomyocytes from different cardiac chamber walls. ( A – D ) Immunofluorescence of TRPM7 (left) and TRPM6 (right) in the left atrium (LA), right atrium (RA), left ventricle (LV), and right ventricle (RV) cardiomyocytes when using Alexa Fluor 488 for the TRPM7 and TRPM6 proteins (stained in green), Alexa Fluor 546 for the F-actin cytoskeleton (stained in red), and Hoechst 33342 for the nuclei (stained in blue). Scale bars indicate 20 µm. ( E ) Example of a negative control, where the primary antibody for TRPM6 and/or TRPM7 is not added, but the cardiomyocyte was subjected to Hoechst 33342 and Alexa Fluor 546. Under such conditions, only immunofluorescence of the nuclei (stained in blue) and F actin cytoskeleton (stained in red) is detected. Note: same cardiomyocyte in the left and right (merged image) panels ( F ) Quantification of the staining intensity of the immunodetected fluorescence of TRPM7 and TRPM6 in the four cardiac chamber walls: LA, RA, LV, and RV. The mean data is provided in arbitrary units (a.u.) (see Table 1 ). A blinded study design (with the origin or treatment of cells unknown to the investigator) was used for the detection of immunofluorescence during the various experimental conditions.
    Anti Trpm6 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpm6 extracellular antibody/product/Alomone Labs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpm6 extracellular antibody - by Bioz Stars, 2021-10
    80/100 stars
      Buy from Supplier

    Image Search Results


    TRPM7/TRPM6 protein expression and TRPM7 kinase activity in splenic T cells. ( A ) Western blot analysis of immunoprecipitated TRPM7 from whole cell lysates of WT and KD splenic T cells. T cells were stimulated with PMA/ionomycin or anti-CD3/CD28 antibody coated beads for 48 hrs. Mouse embryonic fibroblasts were used as a positive control. Equal amounts of protein before immunoprecipitation were ensured by probing for actin. ( B ) Incorporation of 32 P into exogenous myelin basic protein (MBP) by TRPM7 immunoprecipitated from WT and KD resting T cells. Equal quantities of MBP were verified by coomassie blue staining. ( C ) Control experiment showing that anti-TRPM6 antibody was able to recognize TRPM6, by immunoprecipitation using anti-TRPM6 antibody in GFP-TRPM6 transfected HEK cells ( D .

    Journal: Scientific Reports

    Article Title: Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry

    doi: 10.1038/s41598-018-21004-w

    Figure Lengend Snippet: TRPM7/TRPM6 protein expression and TRPM7 kinase activity in splenic T cells. ( A ) Western blot analysis of immunoprecipitated TRPM7 from whole cell lysates of WT and KD splenic T cells. T cells were stimulated with PMA/ionomycin or anti-CD3/CD28 antibody coated beads for 48 hrs. Mouse embryonic fibroblasts were used as a positive control. Equal amounts of protein before immunoprecipitation were ensured by probing for actin. ( B ) Incorporation of 32 P into exogenous myelin basic protein (MBP) by TRPM7 immunoprecipitated from WT and KD resting T cells. Equal quantities of MBP were verified by coomassie blue staining. ( C ) Control experiment showing that anti-TRPM6 antibody was able to recognize TRPM6, by immunoprecipitation using anti-TRPM6 antibody in GFP-TRPM6 transfected HEK cells ( D .

    Article Snippet: Extracts of T cells, mouse embryonic fibroblasts (MEF) or HEK293 cells (untransfected or heterologously expressing GFP tagged human TRPM6 in pEGFP-C1 vector) were obtained as described above for the TRPM7 kinase assay and incubated with anti-TRPM7 antibody (1:500) or anti-TRPM6 antibody (1:500, ACC-046; Alomone labs, Israel) overnight at 4 °C, followed by incubation with protein A sepharose beads for 1 hr.

    Techniques: Expressing, Activity Assay, Western Blot, Immunoprecipitation, Positive Control, Staining, Transfection

    Immunofluorescence staining of CBD-28k ( A ), TRPV5 ( B ), and TRPM6 ( C ) in renal tissue of wild-type and CBD-28k KO mice following CTZ and FSM treatment.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: The role of calbindin-D28k on renal calcium and magnesium handling during treatment with loop and thiazide diuretics

    doi: 10.1152/ajprenal.00057.2015

    Figure Lengend Snippet: Immunofluorescence staining of CBD-28k ( A ), TRPV5 ( B ), and TRPM6 ( C ) in renal tissue of wild-type and CBD-28k KO mice following CTZ and FSM treatment.

    Article Snippet: The membrane was incubated with an anti-TRPV5 antibody (1:1,000, Abcam), anti-CBD-28k antibody (1:20,000, Swant, Marly, Switzerland), anti-TRPM6 antibody (1:1,000, Alomone Laboratory, Jerusalem, Israel), and anti-CLDN16 antibody (1:3,000, Abcam) after blocking with 10% nonfat milk.

    Techniques: Immunofluorescence, Staining, Mouse Assay

    Immunoblotting study of CBD-28k ( A ) TRPV5 ( B ), TRPM6 ( C ), and claudin-16 ( D ) in renal tissue of wild-type and CBD-28k KO mice following CTZ and FSM treatment. The whole blots are shown with molecular size markers labeled on the left . The specific bands

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: The role of calbindin-D28k on renal calcium and magnesium handling during treatment with loop and thiazide diuretics

    doi: 10.1152/ajprenal.00057.2015

    Figure Lengend Snippet: Immunoblotting study of CBD-28k ( A ) TRPV5 ( B ), TRPM6 ( C ), and claudin-16 ( D ) in renal tissue of wild-type and CBD-28k KO mice following CTZ and FSM treatment. The whole blots are shown with molecular size markers labeled on the left . The specific bands

    Article Snippet: The membrane was incubated with an anti-TRPV5 antibody (1:1,000, Abcam), anti-CBD-28k antibody (1:20,000, Swant, Marly, Switzerland), anti-TRPM6 antibody (1:1,000, Alomone Laboratory, Jerusalem, Israel), and anti-CLDN16 antibody (1:3,000, Abcam) after blocking with 10% nonfat milk.

    Techniques: Mouse Assay, Labeling

    TRPM7/TRPM6 protein expression and TRPM7 kinase activity in splenic T cells. ( A ) Western blot analysis of immunoprecipitated TRPM7 from whole cell lysates of WT and KD splenic T cells. T cells were stimulated with PMA/ionomycin or anti-CD3/CD28 antibody coated beads for 48 hrs. Mouse embryonic fibroblasts were used as a positive control. Equal amounts of protein before immunoprecipitation were ensured by probing for actin. ( B ) Incorporation of 32 P into exogenous myelin basic protein (MBP) by TRPM7 immunoprecipitated from WT and KD resting T cells. Equal quantities of MBP were verified by coomassie blue staining. ( C ) Control experiment showing that anti-TRPM6 antibody was able to recognize TRPM6, by immunoprecipitation using anti-TRPM6 antibody in GFP-TRPM6 transfected HEK cells ( D ). Western blot analysis of TRPM6 immunoprecipitated from WT and KD mouse T cells and kidneys. Full gel images are provided in Supplementary Fig. S6 .

    Journal: Scientific Reports

    Article Title: Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry

    doi: 10.1038/s41598-018-21004-w

    Figure Lengend Snippet: TRPM7/TRPM6 protein expression and TRPM7 kinase activity in splenic T cells. ( A ) Western blot analysis of immunoprecipitated TRPM7 from whole cell lysates of WT and KD splenic T cells. T cells were stimulated with PMA/ionomycin or anti-CD3/CD28 antibody coated beads for 48 hrs. Mouse embryonic fibroblasts were used as a positive control. Equal amounts of protein before immunoprecipitation were ensured by probing for actin. ( B ) Incorporation of 32 P into exogenous myelin basic protein (MBP) by TRPM7 immunoprecipitated from WT and KD resting T cells. Equal quantities of MBP were verified by coomassie blue staining. ( C ) Control experiment showing that anti-TRPM6 antibody was able to recognize TRPM6, by immunoprecipitation using anti-TRPM6 antibody in GFP-TRPM6 transfected HEK cells ( D ). Western blot analysis of TRPM6 immunoprecipitated from WT and KD mouse T cells and kidneys. Full gel images are provided in Supplementary Fig. S6 .

    Article Snippet: Extracts of T cells, mouse embryonic fibroblasts (MEF) or HEK293 cells (untransfected or heterologously expressing GFP tagged human TRPM6 in pEGFP-C1 vector) were obtained as described above for the TRPM7 kinase assay and incubated with anti-TRPM7 antibody (1:500) or anti-TRPM6 antibody (1:500, ACC-046; Alomone labs, Israel) overnight at 4 °C, followed by incubation with protein A sepharose beads for 1 hr.

    Techniques: Expressing, Activity Assay, Western Blot, Immunoprecipitation, Positive Control, Staining, Transfection

    Immunofluorescence images suggesting the presence of TRPM6 and TRPM7 proteins in pig cardiomyocytes from different cardiac chamber walls. ( A – D ) Immunofluorescence of TRPM7 (left) and TRPM6 (right) in the left atrium (LA), right atrium (RA), left ventricle (LV), and right ventricle (RV) cardiomyocytes when using Alexa Fluor 488 for the TRPM7 and TRPM6 proteins (stained in green), Alexa Fluor 546 for the F-actin cytoskeleton (stained in red), and Hoechst 33342 for the nuclei (stained in blue). Scale bars indicate 20 µm. ( E ) Example of a negative control, where the primary antibody for TRPM6 and/or TRPM7 is not added, but the cardiomyocyte was subjected to Hoechst 33342 and Alexa Fluor 546. Under such conditions, only immunofluorescence of the nuclei (stained in blue) and F actin cytoskeleton (stained in red) is detected. Note: same cardiomyocyte in the left and right (merged image) panels ( F ) Quantification of the staining intensity of the immunodetected fluorescence of TRPM7 and TRPM6 in the four cardiac chamber walls: LA, RA, LV, and RV. The mean data is provided in arbitrary units (a.u.) (see Table 1 ). A blinded study design (with the origin or treatment of cells unknown to the investigator) was used for the detection of immunofluorescence during the various experimental conditions.

    Journal: International Journal of Molecular Sciences

    Article Title: Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Sensitive TRPM6 and TRPM7-like Current

    doi: 10.3390/ijms22168744

    Figure Lengend Snippet: Immunofluorescence images suggesting the presence of TRPM6 and TRPM7 proteins in pig cardiomyocytes from different cardiac chamber walls. ( A – D ) Immunofluorescence of TRPM7 (left) and TRPM6 (right) in the left atrium (LA), right atrium (RA), left ventricle (LV), and right ventricle (RV) cardiomyocytes when using Alexa Fluor 488 for the TRPM7 and TRPM6 proteins (stained in green), Alexa Fluor 546 for the F-actin cytoskeleton (stained in red), and Hoechst 33342 for the nuclei (stained in blue). Scale bars indicate 20 µm. ( E ) Example of a negative control, where the primary antibody for TRPM6 and/or TRPM7 is not added, but the cardiomyocyte was subjected to Hoechst 33342 and Alexa Fluor 546. Under such conditions, only immunofluorescence of the nuclei (stained in blue) and F actin cytoskeleton (stained in red) is detected. Note: same cardiomyocyte in the left and right (merged image) panels ( F ) Quantification of the staining intensity of the immunodetected fluorescence of TRPM7 and TRPM6 in the four cardiac chamber walls: LA, RA, LV, and RV. The mean data is provided in arbitrary units (a.u.) (see Table 1 ). A blinded study design (with the origin or treatment of cells unknown to the investigator) was used for the detection of immunofluorescence during the various experimental conditions.

    Article Snippet: The cells were permeabilized and incubated with primary rabbit polyclonal anti-TRPM7 (#ACC-047; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-TRPM6 antibody (#ACC-046; Alomone Labs, Jerusalem, Israel) diluted (1:200) in PBS containing 3% BSA in blocking buffer overnight at 4 °C.

    Techniques: Immunofluorescence, Staining, Negative Control, Fluorescence

    Comparison of the expression of TRPM6 and TRPM7 in left ventricular cardiomyocytes incubated for 2 h vs. 12 h in extracellular solutions with ( A , B ) and without ( C , D ) divalent cations (DV and DVF, respectively). ( A , C ) The cardiomyocytes were fixed after 2 h (filled columns) or 12 h (unfilled columns) of cell isolation: Alexa Fluor 488 for the TRPM7 and TRPM6 proteins (stained in green), Alexa Fluor 546 for the F-actin cytoskeleton (stained in red), and Hoechst 33342 for the nuclei (stained in blue). Scale bars indicate 20 µm. ( B , D ) Quantification of the intensity of the fluorescence expressed in arbitrary units (a.u.). # p

    Journal: International Journal of Molecular Sciences

    Article Title: Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Sensitive TRPM6 and TRPM7-like Current

    doi: 10.3390/ijms22168744

    Figure Lengend Snippet: Comparison of the expression of TRPM6 and TRPM7 in left ventricular cardiomyocytes incubated for 2 h vs. 12 h in extracellular solutions with ( A , B ) and without ( C , D ) divalent cations (DV and DVF, respectively). ( A , C ) The cardiomyocytes were fixed after 2 h (filled columns) or 12 h (unfilled columns) of cell isolation: Alexa Fluor 488 for the TRPM7 and TRPM6 proteins (stained in green), Alexa Fluor 546 for the F-actin cytoskeleton (stained in red), and Hoechst 33342 for the nuclei (stained in blue). Scale bars indicate 20 µm. ( B , D ) Quantification of the intensity of the fluorescence expressed in arbitrary units (a.u.). # p

    Article Snippet: The cells were permeabilized and incubated with primary rabbit polyclonal anti-TRPM7 (#ACC-047; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-TRPM6 antibody (#ACC-046; Alomone Labs, Jerusalem, Israel) diluted (1:200) in PBS containing 3% BSA in blocking buffer overnight at 4 °C.

    Techniques: Expressing, Incubation, Cell Isolation, Staining, Fluorescence