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1) Product Images from "Ankyrin-G coordinates assembly of the spectrin-based membrane skeleton, voltage-gated sodium channels, and L1 CAMs at Purkinje neuron initial segments"
Article Title: Ankyrin-G coordinates assembly of the spectrin-based membrane skeleton, voltage-gated sodium channels, and L1 CAMs at Purkinje neuron initial segments
Journal: The Journal of Cell Biology
Figure Legend Snippet: Targeting of β IV spectrin, Na v 1.6, NrCAM, and neurofascin to Purkinje neuron initial segments is disrupted in ankyrin-G cerebellum- specific knockout mice. Cerebellar sections from wild-type (A, D, G, J, and M) or cerebellar ankyrin-G knockout mice (B, C, E, F, H, I, K, L, N, and O) were double labeled with antibodies against calbindin (red in A–F; green in G–L) and either ankyrin-G (green in A–C), βIV spectrin (green in D–F), Na v 1.6 (red in G–I), NrCAM (red in J–L), or neurofascin (M–O). All images except M–O are composites. Bars: (A, B, and E) 5 μm; (C, D, F, G, I, and J–O) 10 μm; and (H) 25 μm.
Techniques Used: Knock-Out, Mouse Assay, Labeling
Figure Legend Snippet: Na v 1.6, ankyrin-G, β IV spectrin, neurofascin, and NrCAM are targeted to axon initial segments of cerebellar Purkinje neurons. Sections of adult rat cerebellum were triple labeled with antibodies against calbindin (blue), ankyrin-G (green), and either Na v 1.6 (B), βIV spectrin (E), neurofascin (H), or NrCAM (K) (red). Composite images are shown in C, F, I, and L. Arrowheads indicate Purkinje cell initial segments. Bars, 10 μm.
Techniques Used: Labeling