Journal: Journal of Neurophysiology
Article Title: Increasing SK2 channel activity impairs associative learning
Figure Lengend Snippet: Trace eyeblink conditioning decreases KCNN2 mRNA and protein levels in the hippocampus. A : KCNN2 protein levels (arbitrary units) as measured by Western analysis. B : KCNN2 mRNA levels normalized to beta 2-microglobulin as measured by qRT-PCR in the hippocampus
Article Snippet: Membranes were probed with one of two SK2 antibodies raised against two independent regions of the full-length SK2 protein (APC-028, 1:500; Alomone Labs, Jerusalem, Israel, aa 542–559; AV35094, Sigma, 1:500, aa 444–493) in 0.2% NFDM, 1% BSA TBS overnight at 4°C, followed by a 1-h incubation at 25°C with a horseradish peroxidase (HRP) conjugated secondary antibody (sc-2313, 1:5,000, Santa Cruz Biotechnology, Santa Cruz, CA) in 0.2% NFDM, 1% BSA TBS-T. Western blots were visualized by enhanced chemiluminescence (Immun-Star HRP, Bio-Rad, Hercules, CA) and developed on film (BioMax, Kodak, Carestream Health, Edison, NJ).
Techniques: Western Blot, Quantitative RT-PCR