immunoblot  (Alomone Labs)


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    Alomone Labs immunoblot
    Immunoblot, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoblot/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoblot - by Bioz Stars, 2022-05
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    Alomone Labs anti kcnn2 antibody
    Trace eyeblink conditioning decreases <t>KCNN2</t> mRNA and protein levels in the hippocampus. A : KCNN2 protein levels (arbitrary units) as measured by Western analysis. B : KCNN2 mRNA levels normalized to beta 2-microglobulin as measured by qRT-PCR in the hippocampus
    Anti Kcnn2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kcnn2 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kcnn2 antibody - by Bioz Stars, 2022-05
    94/100 stars
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    Trace eyeblink conditioning decreases KCNN2 mRNA and protein levels in the hippocampus. A : KCNN2 protein levels (arbitrary units) as measured by Western analysis. B : KCNN2 mRNA levels normalized to beta 2-microglobulin as measured by qRT-PCR in the hippocampus

    Journal: Journal of Neurophysiology

    Article Title: Increasing SK2 channel activity impairs associative learning

    doi: 10.1152/jn.00025.2012

    Figure Lengend Snippet: Trace eyeblink conditioning decreases KCNN2 mRNA and protein levels in the hippocampus. A : KCNN2 protein levels (arbitrary units) as measured by Western analysis. B : KCNN2 mRNA levels normalized to beta 2-microglobulin as measured by qRT-PCR in the hippocampus

    Article Snippet: Membranes were probed with one of two SK2 antibodies raised against two independent regions of the full-length SK2 protein (APC-028, 1:500; Alomone Labs, Jerusalem, Israel, aa 542–559; AV35094, Sigma, 1:500, aa 444–493) in 0.2% NFDM, 1% BSA TBS overnight at 4°C, followed by a 1-h incubation at 25°C with a horseradish peroxidase (HRP) conjugated secondary antibody (sc-2313, 1:5,000, Santa Cruz Biotechnology, Santa Cruz, CA) in 0.2% NFDM, 1% BSA TBS-T. Western blots were visualized by enhanced chemiluminescence (Immun-Star HRP, Bio-Rad, Hercules, CA) and developed on film (BioMax, Kodak, Carestream Health, Edison, NJ).

    Techniques: Western Blot, Quantitative RT-PCR

    BDNF treatment increases SK2 serine phosphorylation. A, B , Western blots of (1) phosphoserine immunoprecipitates from homogenates of control (con) and BDNF-treated hippocampal slices (lanes 1, 2, 5, 6) and (2) COS7 cell lysates (lanes 3/4, 7/8) that had been probed with intact anti-SK2 (Alomone Labs) ( A ) or anti-SK2 that had been preabsorbed with antigen peptide ( B ). As seen in A , two sharp bands of SK2 immunoreactivity within the COS7 lysate (lanes 3/4; at 105 and 45–50 kDa) correspond with phosphoprotein bands in the hippocampal homogenates that become markedly more robust with BDNF treatment. B shows that antigen-preabsorbed antibody no longer detects (1) these bands in the COS7 lysate or (2) enhanced phosphoserine immunoreactivity at these molecular weights in samples of BDNF-treated hippocampal tissue. C , Western blot of SK2 immunoprecipitates from homogenates of control and BDNF-treated slices that were probed with anti-SK2 (Alomone Labs) show that BDNF does not influence levels of total SK2 immunoreactivity; the two lanes at left and right were loaded with 30 and 10 μl of sample, respectively. IP, Immunoprecipitates; WB, whole brain.

    Journal: The Journal of Neuroscience

    Article Title: A Novel Mechanism for the Facilitation of Theta-Induced Long-Term Potentiation by Brain-Derived Neurotrophic Factor

    doi: 10.1523/JNEUROSCI.0800-04.2004

    Figure Lengend Snippet: BDNF treatment increases SK2 serine phosphorylation. A, B , Western blots of (1) phosphoserine immunoprecipitates from homogenates of control (con) and BDNF-treated hippocampal slices (lanes 1, 2, 5, 6) and (2) COS7 cell lysates (lanes 3/4, 7/8) that had been probed with intact anti-SK2 (Alomone Labs) ( A ) or anti-SK2 that had been preabsorbed with antigen peptide ( B ). As seen in A , two sharp bands of SK2 immunoreactivity within the COS7 lysate (lanes 3/4; at 105 and 45–50 kDa) correspond with phosphoprotein bands in the hippocampal homogenates that become markedly more robust with BDNF treatment. B shows that antigen-preabsorbed antibody no longer detects (1) these bands in the COS7 lysate or (2) enhanced phosphoserine immunoreactivity at these molecular weights in samples of BDNF-treated hippocampal tissue. C , Western blot of SK2 immunoprecipitates from homogenates of control and BDNF-treated slices that were probed with anti-SK2 (Alomone Labs) show that BDNF does not influence levels of total SK2 immunoreactivity; the two lanes at left and right were loaded with 30 and 10 μl of sample, respectively. IP, Immunoprecipitates; WB, whole brain.

    Article Snippet: Two different SK2 antisera were used to probe Western blots: rabbit anti-SK2 from Alomone Labs (APC-028) ( ) and rabbit anti-SK2 provided by Hans-Günther Knaus (University of Innsbruck, Innsbruck, Austria) ( ).

    Techniques: Western Blot

    ( A ) shows representative immunofluorescent images of rabbit sinus node cells with SK2 and HCN4 immunolabelling. ( B ) shows the relative expression of SK2 and HCN4. There were no significant changes between the SK2 and HCN4 fluorescence intensity. ( C ) shows fluorescence colocalization analysis. One data point represents one animal. Four cells were evaluated and averaged from each animal. Data are presented as mean ± SEM, applied statistical probe was unpaired Student’s t -test ( p ≤ 0.05).

    Journal: Pharmaceuticals

    Article Title: The Inhibition of the Small-Conductance Ca2+-Activated Potassium Channels Decreases the Sinus Node Pacemaking during Beta-Adrenergic Activation

    doi: 10.3390/ph15030313

    Figure Lengend Snippet: ( A ) shows representative immunofluorescent images of rabbit sinus node cells with SK2 and HCN4 immunolabelling. ( B ) shows the relative expression of SK2 and HCN4. There were no significant changes between the SK2 and HCN4 fluorescence intensity. ( C ) shows fluorescence colocalization analysis. One data point represents one animal. Four cells were evaluated and averaged from each animal. Data are presented as mean ± SEM, applied statistical probe was unpaired Student’s t -test ( p ≤ 0.05).

    Article Snippet: SK2 and HCN4 were labelled with anti-KCa2.2 (SK2) (Alomone, Jerusalem, Israel; 1:50) and anti-HCN4 (Alomone; 1:50) primary antibody overnight at 4 °C.

    Techniques: Expressing, Fluorescence

    Cell membrane protein transport inhibitor dynasore increased the expression of membrane SK2 channel protein and alleviated visceral hypersensitivity. (A) Representative images of cultured HT22 hippocampal neurons in vitro . (B) Effect of dynasore (Dyn) on membrane SK2 channel protein expression in vitro . Western blotting results showed that the Dyn significantly increased the expression of membrane SK2 channel protein in HT22 hippocampal neurons (n = 6 rats each, F 2,15 = 52.91, p

    Journal: Frontiers in Pharmacology

    Article Title: Small-Conductance Ca2+-Activated K+ Channels 2 in the Hypothalamic Paraventricular Nucleus Precipitates Visceral Hypersensitivity Induced by Neonatal Colorectal Distension in Rats

    doi: 10.3389/fphar.2020.605618

    Figure Lengend Snippet: Cell membrane protein transport inhibitor dynasore increased the expression of membrane SK2 channel protein and alleviated visceral hypersensitivity. (A) Representative images of cultured HT22 hippocampal neurons in vitro . (B) Effect of dynasore (Dyn) on membrane SK2 channel protein expression in vitro . Western blotting results showed that the Dyn significantly increased the expression of membrane SK2 channel protein in HT22 hippocampal neurons (n = 6 rats each, F 2,15 = 52.91, p

    Article Snippet: ReagentsThe reagents and antibodies were as follows: rabbit anti-KCa2.2 (APC-028) polyclonal Ab (Alomone Labs, Jerusalem, Israel); mouse anti-GAPDH (AC001) mAb (Abclonal, Woburn, MA, United States); alkaline phosphatase goat anti-rabbit IgG (ZB-2308); alkaline phosphatase horse anti-mouse IgG (ZB-2310); BCA protein assay kit (P0012); sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) sample loading buffer (P0015); BCIP/NBT alkaline phosphatase color development kit (C3206).

    Techniques: Expressing, Cell Culture, In Vitro, Western Blot

    Membrane SK2 channel protein and IAHP were decreased in rats undergoing neonatal CRD. (A) Western blotting data revealed that the PVN SK2 channel protein in membrane fraction was decreased in rats undergoing neonatal and adult CRD vs. rats undergoing adult CRD alone (n = 6 rats each, F 3,20 = 17.25, p

    Journal: Frontiers in Pharmacology

    Article Title: Small-Conductance Ca2+-Activated K+ Channels 2 in the Hypothalamic Paraventricular Nucleus Precipitates Visceral Hypersensitivity Induced by Neonatal Colorectal Distension in Rats

    doi: 10.3389/fphar.2020.605618

    Figure Lengend Snippet: Membrane SK2 channel protein and IAHP were decreased in rats undergoing neonatal CRD. (A) Western blotting data revealed that the PVN SK2 channel protein in membrane fraction was decreased in rats undergoing neonatal and adult CRD vs. rats undergoing adult CRD alone (n = 6 rats each, F 3,20 = 17.25, p

    Article Snippet: ReagentsThe reagents and antibodies were as follows: rabbit anti-KCa2.2 (APC-028) polyclonal Ab (Alomone Labs, Jerusalem, Israel); mouse anti-GAPDH (AC001) mAb (Abclonal, Woburn, MA, United States); alkaline phosphatase goat anti-rabbit IgG (ZB-2308); alkaline phosphatase horse anti-mouse IgG (ZB-2310); BCA protein assay kit (P0012); sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) sample loading buffer (P0015); BCIP/NBT alkaline phosphatase color development kit (C3206).

    Techniques: Western Blot

    PKA activation facilitated the transfer of SK2 channel protein from the cell membrane into the cytoplasm. (A) There was insignificant change in the total PKA expression (n = 6 rats each, F 3,20 = 0.3017, p = 0.8238). (B) The expression of phosphorylated PKA in PVN was significantly increased in rats undergoing neonatal CRD vs. other groups (n = 6 rats each, F 3,20 = 30.44, p

    Journal: Frontiers in Pharmacology

    Article Title: Small-Conductance Ca2+-Activated K+ Channels 2 in the Hypothalamic Paraventricular Nucleus Precipitates Visceral Hypersensitivity Induced by Neonatal Colorectal Distension in Rats

    doi: 10.3389/fphar.2020.605618

    Figure Lengend Snippet: PKA activation facilitated the transfer of SK2 channel protein from the cell membrane into the cytoplasm. (A) There was insignificant change in the total PKA expression (n = 6 rats each, F 3,20 = 0.3017, p = 0.8238). (B) The expression of phosphorylated PKA in PVN was significantly increased in rats undergoing neonatal CRD vs. other groups (n = 6 rats each, F 3,20 = 30.44, p

    Article Snippet: ReagentsThe reagents and antibodies were as follows: rabbit anti-KCa2.2 (APC-028) polyclonal Ab (Alomone Labs, Jerusalem, Israel); mouse anti-GAPDH (AC001) mAb (Abclonal, Woburn, MA, United States); alkaline phosphatase goat anti-rabbit IgG (ZB-2308); alkaline phosphatase horse anti-mouse IgG (ZB-2310); BCA protein assay kit (P0012); sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) sample loading buffer (P0015); BCIP/NBT alkaline phosphatase color development kit (C3206).

    Techniques: Activation Assay, Expressing

    SK2 channel activator 1-EBIO decreased the neuronal firing rates and alleviated visceral pain. Rats receiving intra-PVN injection of 1-EBIO (10 μg/0.3 μL) 30 min before behavioral tests. 1-EBIO prevented the decrease of (A) the pain threshold; (B) the membrane SK2 channel protein in rats undergoing neonatal CRD (n = 6 rats each, F 3,20 = 27.4, p

    Journal: Frontiers in Pharmacology

    Article Title: Small-Conductance Ca2+-Activated K+ Channels 2 in the Hypothalamic Paraventricular Nucleus Precipitates Visceral Hypersensitivity Induced by Neonatal Colorectal Distension in Rats

    doi: 10.3389/fphar.2020.605618

    Figure Lengend Snippet: SK2 channel activator 1-EBIO decreased the neuronal firing rates and alleviated visceral pain. Rats receiving intra-PVN injection of 1-EBIO (10 μg/0.3 μL) 30 min before behavioral tests. 1-EBIO prevented the decrease of (A) the pain threshold; (B) the membrane SK2 channel protein in rats undergoing neonatal CRD (n = 6 rats each, F 3,20 = 27.4, p

    Article Snippet: ReagentsThe reagents and antibodies were as follows: rabbit anti-KCa2.2 (APC-028) polyclonal Ab (Alomone Labs, Jerusalem, Israel); mouse anti-GAPDH (AC001) mAb (Abclonal, Woburn, MA, United States); alkaline phosphatase goat anti-rabbit IgG (ZB-2308); alkaline phosphatase horse anti-mouse IgG (ZB-2310); BCA protein assay kit (P0012); sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) sample loading buffer (P0015); BCIP/NBT alkaline phosphatase color development kit (C3206).

    Techniques: Injection

    A schematic diagram summarizing the hypothesis that PKA-regulated membrane SK2 channel in PVN precipitates neonatal CRD-induced visceral hypersensitivity. Activation of PKA facilitates the internalization of SK2 channel and the decrease of IAHP, thereby increasing the firing rate of CRF neurons in PVN, which precipitates neonatal CRD-induced visceral hypersensitivity.

    Journal: Frontiers in Pharmacology

    Article Title: Small-Conductance Ca2+-Activated K+ Channels 2 in the Hypothalamic Paraventricular Nucleus Precipitates Visceral Hypersensitivity Induced by Neonatal Colorectal Distension in Rats

    doi: 10.3389/fphar.2020.605618

    Figure Lengend Snippet: A schematic diagram summarizing the hypothesis that PKA-regulated membrane SK2 channel in PVN precipitates neonatal CRD-induced visceral hypersensitivity. Activation of PKA facilitates the internalization of SK2 channel and the decrease of IAHP, thereby increasing the firing rate of CRF neurons in PVN, which precipitates neonatal CRD-induced visceral hypersensitivity.

    Article Snippet: ReagentsThe reagents and antibodies were as follows: rabbit anti-KCa2.2 (APC-028) polyclonal Ab (Alomone Labs, Jerusalem, Israel); mouse anti-GAPDH (AC001) mAb (Abclonal, Woburn, MA, United States); alkaline phosphatase goat anti-rabbit IgG (ZB-2308); alkaline phosphatase horse anti-mouse IgG (ZB-2310); BCA protein assay kit (P0012); sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) sample loading buffer (P0015); BCIP/NBT alkaline phosphatase color development kit (C3206).

    Techniques: Activation Assay