bkα  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Anti KCNMA1 KCa1 1 1097 1196 Antibody
    Description:
    Anti KCNMA1 KCa1 1 1097 1196 Antibody APC 021 is a highly specific antibody directed against an epitope of the mouse protein The antibody can be used in western blot immunoprecipitation immunohistochemistry and immunocytochemistry applications It has been designed to recognize KCNMA1 from human mouse and rat samples
    Catalog Number:
    APC-021
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
    Purity:
    The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
    Immunogen:
    GST fusion protein
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Alomone Labs bkα
    Anti KCNMA1 KCa1 1 1097 1196 Antibody
    Anti KCNMA1 KCa1 1 1097 1196 Antibody APC 021 is a highly specific antibody directed against an epitope of the mouse protein The antibody can be used in western blot immunoprecipitation immunohistochemistry and immunocytochemistry applications It has been designed to recognize KCNMA1 from human mouse and rat samples
    https://www.bioz.com/result/bkα/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bkα - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Microtubule structures underlying the sarcoplasmic reticulum support peripheral coupling sites to regulate smooth muscle contractility"

    Article Title: Microtubule structures underlying the sarcoplasmic reticulum support peripheral coupling sites to regulate smooth muscle contractility

    Journal: Science signaling

    doi: 10.1126/scisignal.aan2694

    Depolymerization of microtubules decreases the number of BKα and RyR2 colocalization sites. ( A ) Wide-field image of a freshly isolated arterial myocyte immunolabeled for BKα ( n = 12 cells, n = 3 animals). Red box indicates the area where superresolution images were obtained. Scale bar, 10 μm. ( B ) Superresolution localization map obtained after immunolabeling with anti-BKμ (green) and anti-RyR2 (red) antibodies ( n = 12 cells, n = 3 animals). Scale bar, 3 μm. ROIs (yellow boxes) are shown in magnified view (I) and (II) below. Scale bars, 0.2 μm. ( C ) Cluster size distribution histograms of RyR2 and BKα ( n = 11,005 or n = 11,940 particles for RyR2 and BKα, respectively; 12 cells, n = 3 animals). ( D ) Summary of object-based analysis to determine the number of BKα protein cluster centroids per cell that overlay with RyR2 protein clusters in control cells, cells in which a random BKα distribution has been simulated, and cells treated with nocodazole (10 μM). * P ≤ 0.05 compared to control ( n =12 cells per group, 3 animals). Coloc., colocalization.
    Figure Legend Snippet: Depolymerization of microtubules decreases the number of BKα and RyR2 colocalization sites. ( A ) Wide-field image of a freshly isolated arterial myocyte immunolabeled for BKα ( n = 12 cells, n = 3 animals). Red box indicates the area where superresolution images were obtained. Scale bar, 10 μm. ( B ) Superresolution localization map obtained after immunolabeling with anti-BKμ (green) and anti-RyR2 (red) antibodies ( n = 12 cells, n = 3 animals). Scale bar, 3 μm. ROIs (yellow boxes) are shown in magnified view (I) and (II) below. Scale bars, 0.2 μm. ( C ) Cluster size distribution histograms of RyR2 and BKα ( n = 11,005 or n = 11,940 particles for RyR2 and BKα, respectively; 12 cells, n = 3 animals). ( D ) Summary of object-based analysis to determine the number of BKα protein cluster centroids per cell that overlay with RyR2 protein clusters in control cells, cells in which a random BKα distribution has been simulated, and cells treated with nocodazole (10 μM). * P ≤ 0.05 compared to control ( n =12 cells per group, 3 animals). Coloc., colocalization.

    Techniques Used: Isolation, Immunolabeling

    2) Product Images from "G9a Is Essential for Epigenetic Silencing of K+ Channel Genes in Acute-to-Chronic Pain Transition"

    Article Title: G9a Is Essential for Epigenetic Silencing of K+ Channel Genes in Acute-to-Chronic Pain Transition

    Journal: Nature neuroscience

    doi: 10.1038/nn.4165

    Inhibition of G9a activity normalizes K + channel gene expression in the DRG diminished by nerve injury ( a-d) Effects of intrathecal treatments with vehicle (n = 10), SAHA (50 μg, n = 9), UNC0638 (10 μg, n = 8), GSK503 (5 μg, n = 10), SAHA plus GSK503 (n = 8), SAHA plus UNC0638 (n = 9) or UNC0638 plus GSK503 (n = 8) on the mRNA levels of Kcna4 ( a ), Kcnd2 ( b ), Kcnq2 ( c ), and Kcnma1 ( d ) in the DRG obtained from SNL rats 28 days after surgery. Data from sham control rats were plotted as the control (n = 6 rats). ( e,f ) Effects of nerve injury and UNC0638 on the protein levels of Kv1.4, Kv4.2, Kv7.2 and BKα1 in the L5 and L6 DRG (n = 6 rats in each group). ( g,h ) Effect of G9a-specific siRNA on the G9a and H3K9me2 protein levels in the DRG obtained from SNL rats 24 h after the last injection (n = 5 in each group). ( i,j ) Effects of G9a-specific siRNA on the mRNA levels of G9a, Ezh2, Kcna4, Kcnd2, Kcnq2 and Kcnma1 in the DRG obtained from SNL ( i ) and sham control ( j ) rats 24 h after the last injection (n = 10 in each group). Data are presented as mean ± s.e.m. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc tests ( a-d ), one-way ANOVA ( f,h,i ), or Mann-Whitney test ( j ). * P
    Figure Legend Snippet: Inhibition of G9a activity normalizes K + channel gene expression in the DRG diminished by nerve injury ( a-d) Effects of intrathecal treatments with vehicle (n = 10), SAHA (50 μg, n = 9), UNC0638 (10 μg, n = 8), GSK503 (5 μg, n = 10), SAHA plus GSK503 (n = 8), SAHA plus UNC0638 (n = 9) or UNC0638 plus GSK503 (n = 8) on the mRNA levels of Kcna4 ( a ), Kcnd2 ( b ), Kcnq2 ( c ), and Kcnma1 ( d ) in the DRG obtained from SNL rats 28 days after surgery. Data from sham control rats were plotted as the control (n = 6 rats). ( e,f ) Effects of nerve injury and UNC0638 on the protein levels of Kv1.4, Kv4.2, Kv7.2 and BKα1 in the L5 and L6 DRG (n = 6 rats in each group). ( g,h ) Effect of G9a-specific siRNA on the G9a and H3K9me2 protein levels in the DRG obtained from SNL rats 24 h after the last injection (n = 5 in each group). ( i,j ) Effects of G9a-specific siRNA on the mRNA levels of G9a, Ezh2, Kcna4, Kcnd2, Kcnq2 and Kcnma1 in the DRG obtained from SNL ( i ) and sham control ( j ) rats 24 h after the last injection (n = 10 in each group). Data are presented as mean ± s.e.m. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc tests ( a-d ), one-way ANOVA ( f,h,i ), or Mann-Whitney test ( j ). * P

    Techniques Used: Inhibition, Activity Assay, Expressing, Injection, MANN-WHITNEY

    Related Articles

    Magnetic Beads:

    Article Title: MitoBKCa channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria
    Article Snippet: .. Protein G magnetic beads (100 µl, SureBeads™, Biorad) were washed and saturated with specific polyclonal antibody (4 µg BKCa Ab-Alomone, APC-021) and pre-incubated with 500 µl (2 mg protein) of precleared lysate for 1 h at room temperature. ..

    Incubation:

    Article Title: Nanoscale coupling of junctophilin-2 and ryanodine receptors regulates vascular smooth muscle cell contractility
    Article Snippet: .. Cells were incubated overnight at 4 °C with primary antibodies against JPH2 (Thermo Fisher Scientific 40–5300, 1:400), RyR2 (Thermo Fisher Scientific MA3-916, 1:100), and/or BKα (Alomone Labs APC-021, 1:200) diluted in PBS supplemented with 20% SEA Block, 1% BSA, and 0.05% Triton X. ..

    Blocking Assay:

    Article Title: Nanoscale coupling of junctophilin-2 and ryanodine receptors regulates vascular smooth muscle cell contractility
    Article Snippet: .. Cells were incubated overnight at 4 °C with primary antibodies against JPH2 (Thermo Fisher Scientific 40–5300, 1:400), RyR2 (Thermo Fisher Scientific MA3-916, 1:100), and/or BKα (Alomone Labs APC-021, 1:200) diluted in PBS supplemented with 20% SEA Block, 1% BSA, and 0.05% Triton X. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs bkα
    Depolymerization of microtubules decreases the number of <t>BKα</t> and RyR2 colocalization sites. ( A ) Wide-field image of a freshly isolated arterial myocyte immunolabeled for BKα ( n = 12 cells, n = 3 animals). Red box indicates the area where superresolution images were obtained. Scale bar, 10 μm. ( B ) Superresolution localization map obtained after immunolabeling with anti-BKμ (green) and anti-RyR2 (red) antibodies ( n = 12 cells, n = 3 animals). Scale bar, 3 μm. ROIs (yellow boxes) are shown in magnified view (I) and (II) below. Scale bars, 0.2 μm. ( C ) Cluster size distribution histograms of RyR2 and BKα ( n = 11,005 or n = 11,940 particles for RyR2 and BKα, respectively; 12 cells, n = 3 animals). ( D ) Summary of object-based analysis to determine the number of BKα protein cluster centroids per cell that overlay with RyR2 protein clusters in control cells, cells in which a random BKα distribution has been simulated, and cells treated with nocodazole (10 μM). * P ≤ 0.05 compared to control ( n =12 cells per group, 3 animals). Coloc., colocalization.
    Bkα, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bkα/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bkα - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Depolymerization of microtubules decreases the number of BKα and RyR2 colocalization sites. ( A ) Wide-field image of a freshly isolated arterial myocyte immunolabeled for BKα ( n = 12 cells, n = 3 animals). Red box indicates the area where superresolution images were obtained. Scale bar, 10 μm. ( B ) Superresolution localization map obtained after immunolabeling with anti-BKμ (green) and anti-RyR2 (red) antibodies ( n = 12 cells, n = 3 animals). Scale bar, 3 μm. ROIs (yellow boxes) are shown in magnified view (I) and (II) below. Scale bars, 0.2 μm. ( C ) Cluster size distribution histograms of RyR2 and BKα ( n = 11,005 or n = 11,940 particles for RyR2 and BKα, respectively; 12 cells, n = 3 animals). ( D ) Summary of object-based analysis to determine the number of BKα protein cluster centroids per cell that overlay with RyR2 protein clusters in control cells, cells in which a random BKα distribution has been simulated, and cells treated with nocodazole (10 μM). * P ≤ 0.05 compared to control ( n =12 cells per group, 3 animals). Coloc., colocalization.

    Journal: Science signaling

    Article Title: Microtubule structures underlying the sarcoplasmic reticulum support peripheral coupling sites to regulate smooth muscle contractility

    doi: 10.1126/scisignal.aan2694

    Figure Lengend Snippet: Depolymerization of microtubules decreases the number of BKα and RyR2 colocalization sites. ( A ) Wide-field image of a freshly isolated arterial myocyte immunolabeled for BKα ( n = 12 cells, n = 3 animals). Red box indicates the area where superresolution images were obtained. Scale bar, 10 μm. ( B ) Superresolution localization map obtained after immunolabeling with anti-BKμ (green) and anti-RyR2 (red) antibodies ( n = 12 cells, n = 3 animals). Scale bar, 3 μm. ROIs (yellow boxes) are shown in magnified view (I) and (II) below. Scale bars, 0.2 μm. ( C ) Cluster size distribution histograms of RyR2 and BKα ( n = 11,005 or n = 11,940 particles for RyR2 and BKα, respectively; 12 cells, n = 3 animals). ( D ) Summary of object-based analysis to determine the number of BKα protein cluster centroids per cell that overlay with RyR2 protein clusters in control cells, cells in which a random BKα distribution has been simulated, and cells treated with nocodazole (10 μM). * P ≤ 0.05 compared to control ( n =12 cells per group, 3 animals). Coloc., colocalization.

    Article Snippet: Cells were fixed with 3.2% formaldehyde/0.1% glutaraldehyde–phosphate-buffered saline (PBS), permeabilized and blocked with 0.2% saponin/5% horse serum–PBS, and incubated with primary antibodies against α-tubulin (1:100; MA1-80017, Life Technologies), BKα (1:100; APC-021, Alomone Labs), and RyR2 (1:100; ab2868, Abcam).

    Techniques: Isolation, Immunolabeling