bdnf  (Alomone Labs)


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    Name:
    Anti BDNF Antibody
    Description:
    Anti BDNF Antibody ANT 010 is a highly specific antibody directed against an epitope of the human protein The antibody can be used in western blot immunocytochemistry and immunohistochemistry applications It has been designed to recognize BDNF from rat human and mouse samples The antibody is specific for BDNF it does not crossreact with NGF NT 3 or NT 4
    Catalog Number:
    ANT-010
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs bdnf
    Anti BDNF Antibody
    Anti BDNF Antibody ANT 010 is a highly specific antibody directed against an epitope of the human protein The antibody can be used in western blot immunocytochemistry and immunohistochemistry applications It has been designed to recognize BDNF from rat human and mouse samples The antibody is specific for BDNF it does not crossreact with NGF NT 3 or NT 4
    https://www.bioz.com/result/bdnf/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bdnf - by Bioz Stars, 2021-09
    95/100 stars

    Images

    1) Product Images from "Astrocyte-Derived BDNF Supports Myelin Protein Synthesis after Cuprizone-Induced Demyelination"

    Article Title: Astrocyte-Derived BDNF Supports Myelin Protein Synthesis after Cuprizone-Induced Demyelination

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.4267-13.2014

    Cuprizone-lesioned mice exhibit an increase in BDNF and myelin protein levels 6 h after a single stereotaxic injection of ACPD. a , Western blots demonstrate BDNF, MBP, and MAG protein levels in the corpus callosum of wild-type mice subjected to a 4 week cuprizone lesion and injected with ACPD or 0.9% saline vehicle. GAPDH is shown as a loading control. b , Graphs represent a densitometric analysis of Western blots normalized to GAPDH and are presented as percentage saline-injected control. Levels of mature BDNF are indicated in this analysis. c , Immunohistochemical analysis of MBP, MAG and PLP reactivity in the corpus callosum reveals strong staining intensity in the intact corpus callosum that is decreased following exposure to cuprizone. This MBP, MAG, and PLP deficit is reversed in cuprizone-lesioned animals 6 h after the administration of ACPD. Western blot data analyzed by ANOVA; ***significantly different from saline-injected control at p
    Figure Legend Snippet: Cuprizone-lesioned mice exhibit an increase in BDNF and myelin protein levels 6 h after a single stereotaxic injection of ACPD. a , Western blots demonstrate BDNF, MBP, and MAG protein levels in the corpus callosum of wild-type mice subjected to a 4 week cuprizone lesion and injected with ACPD or 0.9% saline vehicle. GAPDH is shown as a loading control. b , Graphs represent a densitometric analysis of Western blots normalized to GAPDH and are presented as percentage saline-injected control. Levels of mature BDNF are indicated in this analysis. c , Immunohistochemical analysis of MBP, MAG and PLP reactivity in the corpus callosum reveals strong staining intensity in the intact corpus callosum that is decreased following exposure to cuprizone. This MBP, MAG, and PLP deficit is reversed in cuprizone-lesioned animals 6 h after the administration of ACPD. Western blot data analyzed by ANOVA; ***significantly different from saline-injected control at p

    Techniques Used: Mouse Assay, Injection, Western Blot, Immunohistochemistry, Plasmid Purification, Staining

    The ACPD effect on myelin protein expression is blocked when astrocyte-derived BDNF is reduced. Cre recombinase + GFAPcreERT2-floxBDNF-ROSA26 mice and Cre recombinase − floxBDNF-ROSA26 controls were injected with tamoxifen and fed cuprizone-laden food for 4 weeks before they received a single stereotaxic injection of either ACPD or saline vehicle. a , Western blots demonstrate MBP and MAG protein in the corpus callosum of these mice. GAPDH is shown as a loading control. b , Graph represents a densitometric analysis of Western blots normalized to GAPDH and are presented as percentage cre- saline-injected control. Western blot data analyzed by ANOVA; *significantly different from saline-injected, cre- controls at p
    Figure Legend Snippet: The ACPD effect on myelin protein expression is blocked when astrocyte-derived BDNF is reduced. Cre recombinase + GFAPcreERT2-floxBDNF-ROSA26 mice and Cre recombinase − floxBDNF-ROSA26 controls were injected with tamoxifen and fed cuprizone-laden food for 4 weeks before they received a single stereotaxic injection of either ACPD or saline vehicle. a , Western blots demonstrate MBP and MAG protein in the corpus callosum of these mice. GAPDH is shown as a loading control. b , Graph represents a densitometric analysis of Western blots normalized to GAPDH and are presented as percentage cre- saline-injected control. Western blot data analyzed by ANOVA; *significantly different from saline-injected, cre- controls at p

    Techniques Used: Expressing, Derivative Assay, Mouse Assay, Injection, Western Blot

    2) Product Images from "Circadian clock genes and respiratory neuroplasticity genes oscillate in the phrenic motor system"

    Article Title: Circadian clock genes and respiratory neuroplasticity genes oscillate in the phrenic motor system

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    doi: 10.1152/ajpregu.00010.2020

    Immunofluorescence ( A ) and semiquantification ( B ) of BDNF protein expression within phrenic motor neurons retrogradely labeled with cholera toxin beta (CtB) in the ventral C3–C5 spinal cord from rats harvested at the end of the rest phase (ZT11; 5 PM) compared with the end of the active phase (ZT23; 5 AM). BDNF, green; CtB, magenta; a.u., arbitrary units. Scale bar = 100 µm. Values represent means ± SD of n = 11 rats/group). * P
    Figure Legend Snippet: Immunofluorescence ( A ) and semiquantification ( B ) of BDNF protein expression within phrenic motor neurons retrogradely labeled with cholera toxin beta (CtB) in the ventral C3–C5 spinal cord from rats harvested at the end of the rest phase (ZT11; 5 PM) compared with the end of the active phase (ZT23; 5 AM). BDNF, green; CtB, magenta; a.u., arbitrary units. Scale bar = 100 µm. Values represent means ± SD of n = 11 rats/group). * P

    Techniques Used: Immunofluorescence, Expressing, Labeling, CtB Assay

    Related Articles

    Incubation:

    Article Title: Treadmill Exercise Improves Motor Function and Short-term Memory by Enhancing Synaptic Plasticity and Neurogenesis in Photothrombotic Stroke Mice
    Article Snippet: .. The membrane was blocked with skim milk, then membrane was incubated by mouse anti-β-actin antibody (1:1,000; Santa Cruz Biotechnology), rabbit anti-PSD-95 antibody (1:1,000; Abcam, Cambridge, UK), rabbit antisynaptophysin (1:1,000; Abcam), rabbit antiBDNF antibody (1:1,000; Alomone Labs, Jerusalem, Israel), and rabbit anti-TrkB antibody (Santa Cruz Biotechnology) at 4°C during overnight. ..

    Article Title: Sildenafil Alleviates Murine Experimental Autoimmune Encephalomyelitis by Triggering Autophagy in the Spinal Cord
    Article Snippet: .. Briefly, samples were incubated overnight at 4°C with anti-p-CREB (Cell Signaling, #9198, 1:100), anti-BDNF (Alomone, ANT-010, 1:100) and anti-MAP1LC3A/B antibody (Biorad, AHP2167, 1:100). ..

    Article Title: Circadian clock genes and respiratory neuroplasticity genes oscillate in the phrenic motor system
    Article Snippet: .. Sections were incubated overnight at 4°C with the following primary antibodies: BMAL1 (Cell Signaling) or BDNF (Alomone) and CtB (Millipore). ..

    Article Title: Vaccination Prevented Short-Term Memory Loss, but Deteriorated Long-Term Spatial Memory in Alzheimer’s Disease Mice, Independent of Amyloid-β Pathology
    Article Snippet: .. Slices were subsequently incubated for 3 h at 37°C with rabbit anti-BDNF antibody (Alomone, Jerusalem, Israel) in a 1:1000 dilution in PBS containing 1% BSA and 1% normal goat serum. ..

    CtB Assay:

    Article Title: Circadian clock genes and respiratory neuroplasticity genes oscillate in the phrenic motor system
    Article Snippet: .. Sections were incubated overnight at 4°C with the following primary antibodies: BMAL1 (Cell Signaling) or BDNF (Alomone) and CtB (Millipore). ..

    Nucleic Acid Electrophoresis:

    Article Title: The p75NTR neurotrophin receptor is required to organize the mature neuromuscular synapse by regulating synaptic vesicle availability
    Article Snippet: .. For immunoblotting, 20–50 μg of total proteins were loaded in each lane and fractionated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred onto polyvinylidene difluoride (PVDF) membranes (EMD Millipore Corp.), and probed with mouse anti myosin heavy chain (MyHC) (1:1000), mouse anti troponin C (both from Developmental Studies Hybridoma Bank of the University of Iowa, USA), rabbit anti fibronectin (1:5000; Sigma-Aldrich), rabbit anti atrogin-1 (1:500), rabbit anti MuRF-1 (1:500; both from ECM Biosciences, Versailles, KY, USA), mouse anti TrkB (1:500; BD Biosciences, Franklin Lakes, NJ, USA), rabbit anti p-TrkB Y816 (1:500; Merck-Millipore, Burlington, MA, USA), rabbit anti BDNF (1:1000; Alomone Labs, Jerusalem, Israel). ..

    SDS Page:

    Article Title: The p75NTR neurotrophin receptor is required to organize the mature neuromuscular synapse by regulating synaptic vesicle availability
    Article Snippet: .. For immunoblotting, 20–50 μg of total proteins were loaded in each lane and fractionated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred onto polyvinylidene difluoride (PVDF) membranes (EMD Millipore Corp.), and probed with mouse anti myosin heavy chain (MyHC) (1:1000), mouse anti troponin C (both from Developmental Studies Hybridoma Bank of the University of Iowa, USA), rabbit anti fibronectin (1:5000; Sigma-Aldrich), rabbit anti atrogin-1 (1:500), rabbit anti MuRF-1 (1:500; both from ECM Biosciences, Versailles, KY, USA), mouse anti TrkB (1:500; BD Biosciences, Franklin Lakes, NJ, USA), rabbit anti p-TrkB Y816 (1:500; Merck-Millipore, Burlington, MA, USA), rabbit anti BDNF (1:1000; Alomone Labs, Jerusalem, Israel). ..

    Pyrolysis Gas Chromatography:

    Article Title: Myocardial BDNF regulates cardiac bioenergetics through the transcription factor Yin Yang 1
    Article Snippet: .. BDNF antibody was from Alomone Lab (#ANT-010), PGC-1α was from Abcam (#ab106814), and YY1 was from Santa Cruz Biotech (#sc-7341). ..

    Western Blot:

    Article Title: Curdlan Prevents the Cognitive Deficits Induced by a High-Fat Diet in Mice via the Gut-Brain Axis
    Article Snippet: .. Western blot assays were performed using primary antibodies specific for Occludin (ab167161, abcam, United Kingdom, 1: 2,000 dilution), brain derived neurotrophic factor (BDNF) (ANT-010, Alomone labs, Israel, 1:200 dilution), PSD-95 (#3450, Cell Signaling Technology, Boston, MA, United States, 1: 1,000 dilution), and GAPDH (A2077, ABclonal Biotechnology Co., Ltd., United States, 1:2,000 dilution). ..

    Derivative Assay:

    Article Title: Curdlan Prevents the Cognitive Deficits Induced by a High-Fat Diet in Mice via the Gut-Brain Axis
    Article Snippet: .. Western blot assays were performed using primary antibodies specific for Occludin (ab167161, abcam, United Kingdom, 1: 2,000 dilution), brain derived neurotrophic factor (BDNF) (ANT-010, Alomone labs, Israel, 1:200 dilution), PSD-95 (#3450, Cell Signaling Technology, Boston, MA, United States, 1: 1,000 dilution), and GAPDH (A2077, ABclonal Biotechnology Co., Ltd., United States, 1:2,000 dilution). ..

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    Alomone Labs anti bdnf
    Schematics illustrating the possible neuroprotective mechanisms of <t>BDNF</t> in the hyperglycemia-induced neuroinflammation In the hippocampus of the diabetic brain, hyperglycemia leads to microglial activation and increased levels of inflammatory factors, ultimately resulting in synaptic impairments. BDNF can alleviate the hyperglycemia-induced neuroinflammation via specifically inhibiting the aberrant HMGB1/ <t>RAGE/NF-κB</t> signaling pathway.
    Anti Bdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bdnf/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bdnf - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Schematics illustrating the possible neuroprotective mechanisms of BDNF in the hyperglycemia-induced neuroinflammation In the hippocampus of the diabetic brain, hyperglycemia leads to microglial activation and increased levels of inflammatory factors, ultimately resulting in synaptic impairments. BDNF can alleviate the hyperglycemia-induced neuroinflammation via specifically inhibiting the aberrant HMGB1/ RAGE/NF-κB signaling pathway.

    Journal: Aging and Disease

    Article Title: BDNF Alleviates Neuroinflammation in the Hippocampus of Type 1 Diabetic Mice via Blocking the Aberrant HMGB1/RAGE/NF-κB Pathway

    doi: 10.14336/AD.2018.0707

    Figure Lengend Snippet: Schematics illustrating the possible neuroprotective mechanisms of BDNF in the hyperglycemia-induced neuroinflammation In the hippocampus of the diabetic brain, hyperglycemia leads to microglial activation and increased levels of inflammatory factors, ultimately resulting in synaptic impairments. BDNF can alleviate the hyperglycemia-induced neuroinflammation via specifically inhibiting the aberrant HMGB1/ RAGE/NF-κB signaling pathway.

    Article Snippet: The following primary antibodies were used: anti-HMGB1 (1:5000, Abcam, USA), anti-RAGE (1:1000, Abcam, USA), anti-spinophilin (1:1000, Abcam, USA), anti-synaptophysin (1:1000, Sigma, USA), anti-BDNF (1:200, Alomone labs, Israel), anti- NF-κB p65(1:1000, Cell Signaling Technology, USA), anti-p-TrkB (1:400, Abcam, USA), anti-GAPDH (1:1000, Cell Signaling Technology, USA), anti-actin (1:2000, Sigma, USA),anti-tubulin(1:1000, Cell Signaling Technology, USA).

    Techniques: Activation Assay

    BDNF blocked the hyperglycemia-induced decrease in the expression of spinophilin and synaptophysin of diabetic mice (A) Real time PCR analysis of spinophilin. (B) Real time PCR analysis of synaptophysin. (C) Western blot assay of spinophilin. (D) Western blot assay of synaptophysin. Values are means ± SEM. n = 6-7 per group. * p

    Journal: Aging and Disease

    Article Title: BDNF Alleviates Neuroinflammation in the Hippocampus of Type 1 Diabetic Mice via Blocking the Aberrant HMGB1/RAGE/NF-κB Pathway

    doi: 10.14336/AD.2018.0707

    Figure Lengend Snippet: BDNF blocked the hyperglycemia-induced decrease in the expression of spinophilin and synaptophysin of diabetic mice (A) Real time PCR analysis of spinophilin. (B) Real time PCR analysis of synaptophysin. (C) Western blot assay of spinophilin. (D) Western blot assay of synaptophysin. Values are means ± SEM. n = 6-7 per group. * p

    Article Snippet: The following primary antibodies were used: anti-HMGB1 (1:5000, Abcam, USA), anti-RAGE (1:1000, Abcam, USA), anti-spinophilin (1:1000, Abcam, USA), anti-synaptophysin (1:1000, Sigma, USA), anti-BDNF (1:200, Alomone labs, Israel), anti- NF-κB p65(1:1000, Cell Signaling Technology, USA), anti-p-TrkB (1:400, Abcam, USA), anti-GAPDH (1:1000, Cell Signaling Technology, USA), anti-actin (1:2000, Sigma, USA),anti-tubulin(1:1000, Cell Signaling Technology, USA).

    Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot

    BDNF inhibited HMGB1-mediated activation of the RAGE/NF-κB signaling pathway in the hippocampus of diabetic mice (A) Real time PCR analysis of the HMGB1 receptor TLR2. (B) Real time PCR analysis of the HMGB1 receptor TLR4. (C) Real time PCR analysis of the HMGB1 receptor RAGE. (D) Western blot assay of RAGE. (E) Western blot assay of NF-κB p65. (F) Western blot assay of p-NF-κB p65. The values are expressed as means ± SEM. n = 6-7 in each group. * p

    Journal: Aging and Disease

    Article Title: BDNF Alleviates Neuroinflammation in the Hippocampus of Type 1 Diabetic Mice via Blocking the Aberrant HMGB1/RAGE/NF-κB Pathway

    doi: 10.14336/AD.2018.0707

    Figure Lengend Snippet: BDNF inhibited HMGB1-mediated activation of the RAGE/NF-κB signaling pathway in the hippocampus of diabetic mice (A) Real time PCR analysis of the HMGB1 receptor TLR2. (B) Real time PCR analysis of the HMGB1 receptor TLR4. (C) Real time PCR analysis of the HMGB1 receptor RAGE. (D) Western blot assay of RAGE. (E) Western blot assay of NF-κB p65. (F) Western blot assay of p-NF-κB p65. The values are expressed as means ± SEM. n = 6-7 in each group. * p

    Article Snippet: The following primary antibodies were used: anti-HMGB1 (1:5000, Abcam, USA), anti-RAGE (1:1000, Abcam, USA), anti-spinophilin (1:1000, Abcam, USA), anti-synaptophysin (1:1000, Sigma, USA), anti-BDNF (1:200, Alomone labs, Israel), anti- NF-κB p65(1:1000, Cell Signaling Technology, USA), anti-p-TrkB (1:400, Abcam, USA), anti-GAPDH (1:1000, Cell Signaling Technology, USA), anti-actin (1:2000, Sigma, USA),anti-tubulin(1:1000, Cell Signaling Technology, USA).

    Techniques: Activation Assay, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot

    Cuprizone-lesioned mice exhibit an increase in BDNF and myelin protein levels 6 h after a single stereotaxic injection of ACPD. a , Western blots demonstrate BDNF, MBP, and MAG protein levels in the corpus callosum of wild-type mice subjected to a 4 week cuprizone lesion and injected with ACPD or 0.9% saline vehicle. GAPDH is shown as a loading control. b , Graphs represent a densitometric analysis of Western blots normalized to GAPDH and are presented as percentage saline-injected control. Levels of mature BDNF are indicated in this analysis. c , Immunohistochemical analysis of MBP, MAG and PLP reactivity in the corpus callosum reveals strong staining intensity in the intact corpus callosum that is decreased following exposure to cuprizone. This MBP, MAG, and PLP deficit is reversed in cuprizone-lesioned animals 6 h after the administration of ACPD. Western blot data analyzed by ANOVA; ***significantly different from saline-injected control at p

    Journal: The Journal of Neuroscience

    Article Title: Astrocyte-Derived BDNF Supports Myelin Protein Synthesis after Cuprizone-Induced Demyelination

    doi: 10.1523/JNEUROSCI.4267-13.2014

    Figure Lengend Snippet: Cuprizone-lesioned mice exhibit an increase in BDNF and myelin protein levels 6 h after a single stereotaxic injection of ACPD. a , Western blots demonstrate BDNF, MBP, and MAG protein levels in the corpus callosum of wild-type mice subjected to a 4 week cuprizone lesion and injected with ACPD or 0.9% saline vehicle. GAPDH is shown as a loading control. b , Graphs represent a densitometric analysis of Western blots normalized to GAPDH and are presented as percentage saline-injected control. Levels of mature BDNF are indicated in this analysis. c , Immunohistochemical analysis of MBP, MAG and PLP reactivity in the corpus callosum reveals strong staining intensity in the intact corpus callosum that is decreased following exposure to cuprizone. This MBP, MAG, and PLP deficit is reversed in cuprizone-lesioned animals 6 h after the administration of ACPD. Western blot data analyzed by ANOVA; ***significantly different from saline-injected control at p

    Article Snippet: Antibodies for immunohistochemistry included mouse monoclonal antibodies to CC1 (Calbiochem) MBP (Serotec), Neurofilament light-chain (NF-L; Millipore), and GFAP (Millipore); a goat polyclonal antibody to proteolipid protein (PLP; Santa Cruz Biotechnology); rabbit polyclonal antibodies to BDNF (Alomone Labs), HA (Sigma-Aldrich), MAG (Santa Cruz Biotechnology), mGluR1 (Millipore), mGluR5 (Millipore), and mGluR2/3 (Millipore); a chicken polyclonal antibody to GFAP (Millipore); and a rat monoclonal antibody to CD11b (Abcam).

    Techniques: Mouse Assay, Injection, Western Blot, Immunohistochemistry, Plasmid Purification, Staining

    The ACPD effect on myelin protein expression is blocked when astrocyte-derived BDNF is reduced. Cre recombinase + GFAPcreERT2-floxBDNF-ROSA26 mice and Cre recombinase − floxBDNF-ROSA26 controls were injected with tamoxifen and fed cuprizone-laden food for 4 weeks before they received a single stereotaxic injection of either ACPD or saline vehicle. a , Western blots demonstrate MBP and MAG protein in the corpus callosum of these mice. GAPDH is shown as a loading control. b , Graph represents a densitometric analysis of Western blots normalized to GAPDH and are presented as percentage cre- saline-injected control. Western blot data analyzed by ANOVA; *significantly different from saline-injected, cre- controls at p

    Journal: The Journal of Neuroscience

    Article Title: Astrocyte-Derived BDNF Supports Myelin Protein Synthesis after Cuprizone-Induced Demyelination

    doi: 10.1523/JNEUROSCI.4267-13.2014

    Figure Lengend Snippet: The ACPD effect on myelin protein expression is blocked when astrocyte-derived BDNF is reduced. Cre recombinase + GFAPcreERT2-floxBDNF-ROSA26 mice and Cre recombinase − floxBDNF-ROSA26 controls were injected with tamoxifen and fed cuprizone-laden food for 4 weeks before they received a single stereotaxic injection of either ACPD or saline vehicle. a , Western blots demonstrate MBP and MAG protein in the corpus callosum of these mice. GAPDH is shown as a loading control. b , Graph represents a densitometric analysis of Western blots normalized to GAPDH and are presented as percentage cre- saline-injected control. Western blot data analyzed by ANOVA; *significantly different from saline-injected, cre- controls at p

    Article Snippet: Antibodies for immunohistochemistry included mouse monoclonal antibodies to CC1 (Calbiochem) MBP (Serotec), Neurofilament light-chain (NF-L; Millipore), and GFAP (Millipore); a goat polyclonal antibody to proteolipid protein (PLP; Santa Cruz Biotechnology); rabbit polyclonal antibodies to BDNF (Alomone Labs), HA (Sigma-Aldrich), MAG (Santa Cruz Biotechnology), mGluR1 (Millipore), mGluR5 (Millipore), and mGluR2/3 (Millipore); a chicken polyclonal antibody to GFAP (Millipore); and a rat monoclonal antibody to CD11b (Abcam).

    Techniques: Expressing, Derivative Assay, Mouse Assay, Injection, Western Blot

    Immunofluorescence ( A ) and semiquantification ( B ) of BDNF protein expression within phrenic motor neurons retrogradely labeled with cholera toxin beta (CtB) in the ventral C3–C5 spinal cord from rats harvested at the end of the rest phase (ZT11; 5 PM) compared with the end of the active phase (ZT23; 5 AM). BDNF, green; CtB, magenta; a.u., arbitrary units. Scale bar = 100 µm. Values represent means ± SD of n = 11 rats/group). * P

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Circadian clock genes and respiratory neuroplasticity genes oscillate in the phrenic motor system

    doi: 10.1152/ajpregu.00010.2020

    Figure Lengend Snippet: Immunofluorescence ( A ) and semiquantification ( B ) of BDNF protein expression within phrenic motor neurons retrogradely labeled with cholera toxin beta (CtB) in the ventral C3–C5 spinal cord from rats harvested at the end of the rest phase (ZT11; 5 PM) compared with the end of the active phase (ZT23; 5 AM). BDNF, green; CtB, magenta; a.u., arbitrary units. Scale bar = 100 µm. Values represent means ± SD of n = 11 rats/group). * P

    Article Snippet: Sections were incubated overnight at 4°C with the following primary antibodies: BMAL1 (Cell Signaling) or BDNF (Alomone) and CtB (Millipore).

    Techniques: Immunofluorescence, Expressing, Labeling, CtB Assay

    Effects of chronic curdlan supplementation on synaptic morphology and synaptic proteins in the PFC and hippocampus of mice fed a HF diet. (A,B) Electron micrograph of synaptic ultrastructure in the PFC (A) and hippocampus CA1 region (B) ; (C,D) image analysis of thickness of postsynaptic densities (PSD), width of synaptic cleft and curvature of synaptic interface in the PFC (C) , and hippocampus (D) ; (E–G) protein expression levels of brain derived neurotrophic factor (BDNF) and PSD-95 in the PFC (E,F) and hippocampus (G,H) . Values are mean ± standard error of means. n = 5. * p

    Journal: Frontiers in Neuroscience

    Article Title: Curdlan Prevents the Cognitive Deficits Induced by a High-Fat Diet in Mice via the Gut-Brain Axis

    doi: 10.3389/fnins.2020.00384

    Figure Lengend Snippet: Effects of chronic curdlan supplementation on synaptic morphology and synaptic proteins in the PFC and hippocampus of mice fed a HF diet. (A,B) Electron micrograph of synaptic ultrastructure in the PFC (A) and hippocampus CA1 region (B) ; (C,D) image analysis of thickness of postsynaptic densities (PSD), width of synaptic cleft and curvature of synaptic interface in the PFC (C) , and hippocampus (D) ; (E–G) protein expression levels of brain derived neurotrophic factor (BDNF) and PSD-95 in the PFC (E,F) and hippocampus (G,H) . Values are mean ± standard error of means. n = 5. * p

    Article Snippet: Western blot assays were performed using primary antibodies specific for Occludin (ab167161, abcam, United Kingdom, 1: 2,000 dilution), brain derived neurotrophic factor (BDNF) (ANT-010, Alomone labs, Israel, 1:200 dilution), PSD-95 (#3450, Cell Signaling Technology, Boston, MA, United States, 1: 1,000 dilution), and GAPDH (A2077, ABclonal Biotechnology Co., Ltd., United States, 1:2,000 dilution).

    Techniques: Mouse Assay, Expressing, Derivative Assay