p75ntr  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Alomone Labs p75ntr
    Characterization of ENCDC cultures. Flow cytometry of ENCDC cultures stained with antibodies against the immature neuronal marker, p75 NTR+ ‐FITC demonstrated 95.1% p75 NTR+ ‐positive, 97.3% viability in subculture 3, and 96.1% p75 NTR+ ‐positive, 98.2% viability in subculture 4 (a). Proportion of ENCDC cultures characterized for expression of <t>p75NTR+,</t> pH3, 5‐HT, nNOS, and TH. Scale bar = 20 µm (b). There was a significant increase in the proportion of serotonergic neurons and nitrergic neurons in subculture 5 (gray) compared to subculture 4 (black), but no significant difference in proliferating cells or dopaminergic neurons across the cultures. *p ≤ .05. Values are presented as mean ± SEM (c)
    P75ntr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p75ntr/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p75ntr - by Bioz Stars, 2022-05
    92/100 stars

    Images

    1) Product Images from "Influence of bacterial components on the developmental programming of enteric neurons, et al. Influence of bacterial components on the developmental programming of enteric neurons"

    Article Title: Influence of bacterial components on the developmental programming of enteric neurons, et al. Influence of bacterial components on the developmental programming of enteric neurons

    Journal: Physiological Reports

    doi: 10.14814/phy2.14611

    Characterization of ENCDC cultures. Flow cytometry of ENCDC cultures stained with antibodies against the immature neuronal marker, p75 NTR+ ‐FITC demonstrated 95.1% p75 NTR+ ‐positive, 97.3% viability in subculture 3, and 96.1% p75 NTR+ ‐positive, 98.2% viability in subculture 4 (a). Proportion of ENCDC cultures characterized for expression of p75NTR+, pH3, 5‐HT, nNOS, and TH. Scale bar = 20 µm (b). There was a significant increase in the proportion of serotonergic neurons and nitrergic neurons in subculture 5 (gray) compared to subculture 4 (black), but no significant difference in proliferating cells or dopaminergic neurons across the cultures. *p ≤ .05. Values are presented as mean ± SEM (c)
    Figure Legend Snippet: Characterization of ENCDC cultures. Flow cytometry of ENCDC cultures stained with antibodies against the immature neuronal marker, p75 NTR+ ‐FITC demonstrated 95.1% p75 NTR+ ‐positive, 97.3% viability in subculture 3, and 96.1% p75 NTR+ ‐positive, 98.2% viability in subculture 4 (a). Proportion of ENCDC cultures characterized for expression of p75NTR+, pH3, 5‐HT, nNOS, and TH. Scale bar = 20 µm (b). There was a significant increase in the proportion of serotonergic neurons and nitrergic neurons in subculture 5 (gray) compared to subculture 4 (black), but no significant difference in proliferating cells or dopaminergic neurons across the cultures. *p ≤ .05. Values are presented as mean ± SEM (c)

    Techniques Used: Flow Cytometry, Staining, Marker, Expressing

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Alomone Labs anti cd271
    Effective and functional Notch3 KD depletes MSLCs and VM-engaging melanoma cells in 1205 Lu xenografts. A. Western blot analyses demonstrated that stem cell markers (e.g., CD133 and <t>CD271)</t> as well as VM maker, CD144, were down-regulated in Notch3 KD 1205 Lu tumors, compared to non-target control (A). Consistent with the observed VM inhibition by Notch3 KD above, the expression of Tie-1, a VM-associated gene, was also down-regulated in Nocth3 KD 1205Lu xenografts by qRT-PCR (B). Of note, the efficacy of Notch3 KD was maintained in the tumor xenografts as shown by Western blotting (A). On the other hand, in A375 melanoma cells where no change in tumorigenicity was appreciated following Notch3 KD, the expression of stem-like cell marker, CD271, was not affected (C). Attempts to further validate CD133 and CD144 in Notch3 KD A375 xenografts using Western blot analysis (C8161 lysate included as a positive control) were unsuccessful due to sensitivity issue ( Fig. 6C ; note that CD133 and CD144 signals were undetectable both in the control and KD xenografts despite maximal loading and prolonged overnight exposure). *, P
    Anti Cd271, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd271/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd271 - by Bioz Stars, 2022-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    Effective and functional Notch3 KD depletes MSLCs and VM-engaging melanoma cells in 1205 Lu xenografts. A. Western blot analyses demonstrated that stem cell markers (e.g., CD133 and CD271) as well as VM maker, CD144, were down-regulated in Notch3 KD 1205 Lu tumors, compared to non-target control (A). Consistent with the observed VM inhibition by Notch3 KD above, the expression of Tie-1, a VM-associated gene, was also down-regulated in Nocth3 KD 1205Lu xenografts by qRT-PCR (B). Of note, the efficacy of Notch3 KD was maintained in the tumor xenografts as shown by Western blotting (A). On the other hand, in A375 melanoma cells where no change in tumorigenicity was appreciated following Notch3 KD, the expression of stem-like cell marker, CD271, was not affected (C). Attempts to further validate CD133 and CD144 in Notch3 KD A375 xenografts using Western blot analysis (C8161 lysate included as a positive control) were unsuccessful due to sensitivity issue ( Fig. 6C ; note that CD133 and CD144 signals were undetectable both in the control and KD xenografts despite maximal loading and prolonged overnight exposure). *, P

    Journal: Laboratory investigation; a journal of technical methods and pathology

    Article Title: Notch3 Signaling-Mediated Melanoma-Endothelial Crosstalk Regulates Melanoma Stem-Like Cell Homeostasis and Niche Morphogenesis

    doi: 10.1038/labinvest.2017.1

    Figure Lengend Snippet: Effective and functional Notch3 KD depletes MSLCs and VM-engaging melanoma cells in 1205 Lu xenografts. A. Western blot analyses demonstrated that stem cell markers (e.g., CD133 and CD271) as well as VM maker, CD144, were down-regulated in Notch3 KD 1205 Lu tumors, compared to non-target control (A). Consistent with the observed VM inhibition by Notch3 KD above, the expression of Tie-1, a VM-associated gene, was also down-regulated in Nocth3 KD 1205Lu xenografts by qRT-PCR (B). Of note, the efficacy of Notch3 KD was maintained in the tumor xenografts as shown by Western blotting (A). On the other hand, in A375 melanoma cells where no change in tumorigenicity was appreciated following Notch3 KD, the expression of stem-like cell marker, CD271, was not affected (C). Attempts to further validate CD133 and CD144 in Notch3 KD A375 xenografts using Western blot analysis (C8161 lysate included as a positive control) were unsuccessful due to sensitivity issue ( Fig. 6C ; note that CD133 and CD144 signals were undetectable both in the control and KD xenografts despite maximal loading and prolonged overnight exposure). *, P

    Article Snippet: Blots were probed with anti-Notch3 (Cell Signaling Technology, D1118 rabbit mAb, Danvers, MA), anti-CD133 (Miltenyi Biotech Inc., clone W6B3C1), anti-CD271 (Alomone Labs, Jerusalem, Israel), anti-CD144 (Cell Signaling Technology) Abs.

    Techniques: Functional Assay, Western Blot, Inhibition, Expressing, Quantitative RT-PCR, Marker, Positive Control

    VEGF-A KD increases VM and MSLC markers in WM1617 melanoma xenografts A, Expression profiling by qRT-PCR revealed concomitant CD144, CD133 and CD271 upregulation in WM1617 VEGF-A KD (sh1) xenografts compared to nontarget controls. A concomitant increase in CD133 and CD271, but not CD144 was also observed in C8161 VEGF-A KD (sh1) xenografts compared to nontarget controls. No change in CD144, CD133 or CD271 expression was observed in A2058 VEGF-A KD (sh1) xenografts compared to nontarget controls. No change in these markers was observed in WM1617, C8161 or A2058 VEGF-A KD (sh1) cells compared to nontarget controls in vitro . *, P

    Journal: Cancer research

    Article Title: Induction of vasculogenic mimicry overrides VEGF-A silencing and enriches stem-like cancer cells in melanoma

    doi: 10.1158/0008-5472.CAN-14-1855

    Figure Lengend Snippet: VEGF-A KD increases VM and MSLC markers in WM1617 melanoma xenografts A, Expression profiling by qRT-PCR revealed concomitant CD144, CD133 and CD271 upregulation in WM1617 VEGF-A KD (sh1) xenografts compared to nontarget controls. A concomitant increase in CD133 and CD271, but not CD144 was also observed in C8161 VEGF-A KD (sh1) xenografts compared to nontarget controls. No change in CD144, CD133 or CD271 expression was observed in A2058 VEGF-A KD (sh1) xenografts compared to nontarget controls. No change in these markers was observed in WM1617, C8161 or A2058 VEGF-A KD (sh1) cells compared to nontarget controls in vitro . *, P

    Article Snippet: Membranes were probed overnight at 4°C with mouse anti-CD133 (Miltenyi Biotech Inc, clone W6B3C1, San Diego, CA) at 1:200, rabbit anti-CD271 (Alomone, Jerusalem, Israel) at 1:1000, rabbit anti-CD144 (Cell Signaling Technology, Danvers, MA) at 1:1000, or mouse anti-beta-actin (Abcam, Cambridge, MA) at 1:5000 followed by probing with the appropriate secondary antibody conjugated to horseradish peroxidase (Jackson Immunoresearch, West Grove, PA).

    Techniques: Expressing, Quantitative RT-PCR, In Vitro

    Characterization of ENCDC cultures. Flow cytometry of ENCDC cultures stained with antibodies against the immature neuronal marker, p75 NTR+ ‐FITC demonstrated 95.1% p75 NTR+ ‐positive, 97.3% viability in subculture 3, and 96.1% p75 NTR+ ‐positive, 98.2% viability in subculture 4 (a). Proportion of ENCDC cultures characterized for expression of p75NTR+, pH3, 5‐HT, nNOS, and TH. Scale bar = 20 µm (b). There was a significant increase in the proportion of serotonergic neurons and nitrergic neurons in subculture 5 (gray) compared to subculture 4 (black), but no significant difference in proliferating cells or dopaminergic neurons across the cultures. *p ≤ .05. Values are presented as mean ± SEM (c)

    Journal: Physiological Reports

    Article Title: Influence of bacterial components on the developmental programming of enteric neurons, et al. Influence of bacterial components on the developmental programming of enteric neurons

    doi: 10.14814/phy2.14611

    Figure Lengend Snippet: Characterization of ENCDC cultures. Flow cytometry of ENCDC cultures stained with antibodies against the immature neuronal marker, p75 NTR+ ‐FITC demonstrated 95.1% p75 NTR+ ‐positive, 97.3% viability in subculture 3, and 96.1% p75 NTR+ ‐positive, 98.2% viability in subculture 4 (a). Proportion of ENCDC cultures characterized for expression of p75NTR+, pH3, 5‐HT, nNOS, and TH. Scale bar = 20 µm (b). There was a significant increase in the proportion of serotonergic neurons and nitrergic neurons in subculture 5 (gray) compared to subculture 4 (black), but no significant difference in proliferating cells or dopaminergic neurons across the cultures. *p ≤ .05. Values are presented as mean ± SEM (c)

    Article Snippet: 2.3 Flow cytometry Cultured cells were stained with p75NTR+‐FITC (Alomone Labs ANT‐007F) and 7‐Aminoactinomycin D (7‐AAD; Invitrogen 00–6993–50).

    Techniques: Flow Cytometry, Staining, Marker, Expressing