anti p75ntr extracellular antibody (Alomone Labs)
Alomone Labs is a verified supplier 
Alomone Labs manufactures this product 
93
Structured Review
Alomone Labs
anti p75ntr extracellular antibody
Anti P75ntr Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p75ntr extracellular antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Anti P75ntr Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p75ntr extracellular antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti p75ntr extracellular antibody - by Bioz Stars,
2023-09
93/100 stars
Images
1) Product Images from "Upregulation of p75NTR by Histone Deacetylase Inhibitors Sensitizes Human Neuroblastoma Cells to Targeted Immunotoxin-Induced Apoptosis"
Article Title: Upregulation of p75NTR by Histone Deacetylase Inhibitors Sensitizes Human Neuroblastoma Cells to Targeted Immunotoxin-Induced Apoptosis
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms23073849
Figure Legend Snippet: Induction of p75NTR expression in human neuroblastoma cell lines by VPA and entinostat. ( A ) The cells were exposed for 24 h to either vehicle, 1 mM VPA, or 1 µM entinostat (entin) and then analyzed for p75NTR protein levels by Western blot. The values are the mean ± SD of four independent experiments. The position of molecular mass standards is reported in the right side of the blots. *** p < 0.001 vs. control (vehicle) by Student’s t test. ( B ) SH-SY5Y and LAN-1 cells were treated as indicated in ( A ) and then analyzed for CASZ1 protein levels. The values are the mean ± SD of four (SH-SY5Y) and three (LAN-1) experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control (vehicle). # p < 0.05, ## p < 0.01 vs. control (vehicle) by ANOVA followed by Tukey’s test. ( C ) SH-SY5Y and Kelly cells were incubated for 24 h with either vehicle or the indicated concentrations of entinostat. The cell lysates were analyzed for p75NTR protein expression. Values are the mean ± SD of three experiments. * p < 0.05, *** p < 0.001 vs. control (vehicle) by Student’s t test.
Techniques Used: Expressing, Western Blot, Incubation
Figure Legend Snippet: Enhancement of cell surface p75NTR expression by VPA and entinostat. ( A ) The SH-SY5Y cells were incubated for 24 h with either vehicle, 1 mM VPA, or 1 µM entinostat. Thereafter, the cells were treated with the cell impermeant biotinylating agent sulfosuccinimidyl-6-(biotin-amido) hexanoate and the solubilized proteins were isolated by precipitation with streptavidin-conjugated agarose beads. The total cell extract (cell lysate) and precipitated proteins (surface protein) were analyzed for p75NTR by Western blot. The bar graphs report the changes in the cell surface p75NTR levels that were normalized normalized to pan-cadherin (cadherin), a plasma membrane marker. Values are the mean ± SD of three independent experiments. *** p < 0.001 vs. vehicle by Student’s t -test. ( B ) SH-SY5Y cells that were grown on glass coverslips were treated for 24 h with either vehicle ( a ) or 1 µM entinostat (entin) ( b , c ), fixed, and incubated overnight with ATTO-488-conjugated anti-p75NTR (extracellular) antibody. In ( c ) the cells were preincubated with a mouse monoclonal unconjugated antibody directed against the extracellular domain of the receptor before the addition of the labeled antibody. The images were analyzed for p75NTR expression (green color) by fluorescence microscopy. The nuclei were stained in blue with 4′-6-diamidino-2phenylindole dihydrochloride (DAPI). Scale bar = 25 µm. The values that are shown in the scatter plot are the mean ± SD of three independent experiments. *** p < 0.001 vs. vehicle by Student’s t -test.
Techniques Used: Expressing, Incubation, Isolation, Western Blot, Marker, Labeling, Fluorescence, Microscopy, Staining
Figure Legend Snippet: Exposure to p75IgG-Sap induces p75NTR internalization, intracellular saporin-S6 delivery, and citotoxicity in entinostat-treated SH-SY5Y neuroblastoma cells. ( A ) The cells were treated for 24 h with 1 µM entinostat and the cell lysates were analyzed for p75NTR immunoreactivity by Western blot using either a non-targeted preimmune antibody conjugated to saporin-S6 (IgG-Sap) (1:50) or p75IgG-Sap (1:50). The images are representative of three separate experiments. ( B ). The cells were incubated for 24 h with either vehicle or the indicated concentrations of entinostat (entin) and then exposed for additional 24 h with either vehicle or p75IgG-Sap (30 nM). Biotinylated cell surface proteins were isolated and analyzed for p75NTR expression. The values are the mean ± SD of three independent experiments. *** p < 0.001 vs. vehicle; ### , p < 0.001 by ANOVA followed by by Tukey’s test. ( C ) The cells were treated with 1 µM entinostat for 24 h and then exposed to either p75IgG-Sap (30 nM) or IgG-Sap (30 nM) for additional 24 h. The cells were then analyzed for p75NTR (green color) and LAMP1 (red color) localization by immunofluorescence. ( D ) The cells were incubated for 24 h with either vehicle or entinostat (1 µM), exposed to p75IgG-Sap (30 nM) for additional 24 h, and then analyzed for saporin-S6 (green color) and LAMP1 (red color) expression by immunofluorescence. In ( C , D ) the cell nuclei were stained in blue with DAPI. Scale bar = 25 µm. Values that are reported in the scatter plots are the mean ± SD of three independent experiments # p < 0.05; ## p < 0.01 by Student’s t -test. ( E ) The cells were treated for 24 h with either vehicle or 1 µM entinostat and subsequently exposed to the indicated concentrations of p75IgG-Sap for 24 h. Cell viability was assayed by propidium iodide (PI) staining (red color). The values are the mean ± SD of five independent experiments. * p < 0.05 vs. vehicle; # p < 0.05, ### p < 0.001 by ANOVA followed by Tukey’s test. ( F ) The cells were treated for 24 h with either vehicle or 1 µM entinostat and then exposed to either vehicle or 30 nM p75IgG-Sap. The images were obtained by phase-contrast microscopy and are representative of five separate experiments. ( G – J ) Cell viability was determined by a luminescence assay; ( G ) The cells were treated as indicated in ( F ); ( H , I ) The cells were treated with either vehicle or 1 µM entinostat and then incubated for 24 h with vehicle, 30 nM IgG-Sap, or 30 nM saporin-S6 (Sap); ( J ) The cells were treated for 24 h with either vehicle or 1 µM entinostat and then incubated with 30 nM p75IgG-Sap in the absence and in the presence of 100 nM p75IgG added 2 h before the immunotoxin. The values are expressed as percent of control (vehicle) the mean ± SD of four independent experiments. ** p < 0.01, *** p < 0.001 vs. vehicle; ### p < 0.001, ns = not significant by ANOVA followed by Tukey’s test.
Techniques Used: Western Blot, Incubation, Isolation, Expressing, Immunofluorescence, Staining, Microscopy, Luminescence Assay
Figure Legend Snippet: Upregulation of p75NTR and induction of apoptosis by entinostat in neuroblastoma spheroids. ( A , D ) Light microscopy images of IMR-32 and SH-SY5Y multicell spheroids (mcs) that were incubated for 72 h with either vehicle or 1 µM entinostat. The scatter plots report the values of mcs sizes expressed as percent of control (vehicle). Scale bar = 200 µm. ( B , C , E , F ) The spheroids were treated as indicated in ( A , D ) and then analyzed for p75NTR levels and PARP cleavage by Western blot. The values are the mean ± SD of four individual experiments. ** p < 0.01, *** p < 0.001 vs. vehicle by Student’s t -test.
Techniques Used: Light Microscopy, Incubation, Western Blot
Figure Legend Snippet: Expression of p75NTR and histone H3 acetylation in neuroblastoma tumor xenografts and different organs of entinostat-treated mice. ( A , C – F ) Athymic nude mice bearing xenograft of SH-SY5Y cells were treated daily for 10 days with either the vehicle or entinostat (20 mg/kg) by oral gavage. The mice were sacrificed, the tumor xenografts and the indicated organs were resected, and the tissue extracts were analyzed for p75NTR levels by Western blot. ( B ) The levels of acetylated histone H3 were determined in neuroblastoma tumor xenografts of vehicle- and entinostat-treated mice by Western blot. Each lane was loaded with a sample that was obtained from an individual animal (in ( A , B ): four animals that were treated with vehicle and five animals that were treated with entinostat; in ( C – F ), three animals that were treated with either vehicle or entinostat). Scatter plots indicate the absolute values of densitometric ratios and are the mean ± SD of three independent Western blots. * p < 0.05, *** p < 0.001, ns = not significant by Student’s t test.
Techniques Used: Expressing, Western Blot
anti p75ntr extracellular antibody (Alomone Labs)
Alomone Labs is a verified supplier
Alomone Labs manufactures this product
93
Structured Review
Alomone Labs
anti p75ntr extracellular antibody
Anti P75ntr Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p75ntr extracellular antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Anti P75ntr Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p75ntr extracellular antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti p75ntr extracellular antibody - by Bioz Stars,
2023-09
93/100 stars
Images
1) Product Images from "Upregulation of p75NTR by Histone Deacetylase Inhibitors Sensitizes Human Neuroblastoma Cells to Targeted Immunotoxin-Induced Apoptosis"
Article Title: Upregulation of p75NTR by Histone Deacetylase Inhibitors Sensitizes Human Neuroblastoma Cells to Targeted Immunotoxin-Induced Apoptosis
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms23073849
Figure Legend Snippet: Induction of p75NTR expression in human neuroblastoma cell lines by VPA and entinostat. ( A ) The cells were exposed for 24 h to either vehicle, 1 mM VPA, or 1 µM entinostat (entin) and then analyzed for p75NTR protein levels by Western blot. The values are the mean ± SD of four independent experiments. The position of molecular mass standards is reported in the right side of the blots. *** p < 0.001 vs. control (vehicle) by Student’s t test. ( B ) SH-SY5Y and LAN-1 cells were treated as indicated in ( A ) and then analyzed for CASZ1 protein levels. The values are the mean ± SD of four (SH-SY5Y) and three (LAN-1) experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control (vehicle). # p < 0.05, ## p < 0.01 vs. control (vehicle) by ANOVA followed by Tukey’s test. ( C ) SH-SY5Y and Kelly cells were incubated for 24 h with either vehicle or the indicated concentrations of entinostat. The cell lysates were analyzed for p75NTR protein expression. Values are the mean ± SD of three experiments. * p < 0.05, *** p < 0.001 vs. control (vehicle) by Student’s t test.
Techniques Used: Expressing, Western Blot, Incubation
Figure Legend Snippet: Enhancement of cell surface p75NTR expression by VPA and entinostat. ( A ) The SH-SY5Y cells were incubated for 24 h with either vehicle, 1 mM VPA, or 1 µM entinostat. Thereafter, the cells were treated with the cell impermeant biotinylating agent sulfosuccinimidyl-6-(biotin-amido) hexanoate and the solubilized proteins were isolated by precipitation with streptavidin-conjugated agarose beads. The total cell extract (cell lysate) and precipitated proteins (surface protein) were analyzed for p75NTR by Western blot. The bar graphs report the changes in the cell surface p75NTR levels that were normalized normalized to pan-cadherin (cadherin), a plasma membrane marker. Values are the mean ± SD of three independent experiments. *** p < 0.001 vs. vehicle by Student’s t -test. ( B ) SH-SY5Y cells that were grown on glass coverslips were treated for 24 h with either vehicle ( a ) or 1 µM entinostat (entin) ( b , c ), fixed, and incubated overnight with ATTO-488-conjugated anti-p75NTR (extracellular) antibody. In ( c ) the cells were preincubated with a mouse monoclonal unconjugated antibody directed against the extracellular domain of the receptor before the addition of the labeled antibody. The images were analyzed for p75NTR expression (green color) by fluorescence microscopy. The nuclei were stained in blue with 4′-6-diamidino-2phenylindole dihydrochloride (DAPI). Scale bar = 25 µm. The values that are shown in the scatter plot are the mean ± SD of three independent experiments. *** p < 0.001 vs. vehicle by Student’s t -test.
Techniques Used: Expressing, Incubation, Isolation, Western Blot, Marker, Labeling, Fluorescence, Microscopy, Staining
Figure Legend Snippet: Exposure to p75IgG-Sap induces p75NTR internalization, intracellular saporin-S6 delivery, and citotoxicity in entinostat-treated SH-SY5Y neuroblastoma cells. ( A ) The cells were treated for 24 h with 1 µM entinostat and the cell lysates were analyzed for p75NTR immunoreactivity by Western blot using either a non-targeted preimmune antibody conjugated to saporin-S6 (IgG-Sap) (1:50) or p75IgG-Sap (1:50). The images are representative of three separate experiments. ( B ). The cells were incubated for 24 h with either vehicle or the indicated concentrations of entinostat (entin) and then exposed for additional 24 h with either vehicle or p75IgG-Sap (30 nM). Biotinylated cell surface proteins were isolated and analyzed for p75NTR expression. The values are the mean ± SD of three independent experiments. *** p < 0.001 vs. vehicle; ### , p < 0.001 by ANOVA followed by by Tukey’s test. ( C ) The cells were treated with 1 µM entinostat for 24 h and then exposed to either p75IgG-Sap (30 nM) or IgG-Sap (30 nM) for additional 24 h. The cells were then analyzed for p75NTR (green color) and LAMP1 (red color) localization by immunofluorescence. ( D ) The cells were incubated for 24 h with either vehicle or entinostat (1 µM), exposed to p75IgG-Sap (30 nM) for additional 24 h, and then analyzed for saporin-S6 (green color) and LAMP1 (red color) expression by immunofluorescence. In ( C , D ) the cell nuclei were stained in blue with DAPI. Scale bar = 25 µm. Values that are reported in the scatter plots are the mean ± SD of three independent experiments # p < 0.05; ## p < 0.01 by Student’s t -test. ( E ) The cells were treated for 24 h with either vehicle or 1 µM entinostat and subsequently exposed to the indicated concentrations of p75IgG-Sap for 24 h. Cell viability was assayed by propidium iodide (PI) staining (red color). The values are the mean ± SD of five independent experiments. * p < 0.05 vs. vehicle; # p < 0.05, ### p < 0.001 by ANOVA followed by Tukey’s test. ( F ) The cells were treated for 24 h with either vehicle or 1 µM entinostat and then exposed to either vehicle or 30 nM p75IgG-Sap. The images were obtained by phase-contrast microscopy and are representative of five separate experiments. ( G – J ) Cell viability was determined by a luminescence assay; ( G ) The cells were treated as indicated in ( F ); ( H , I ) The cells were treated with either vehicle or 1 µM entinostat and then incubated for 24 h with vehicle, 30 nM IgG-Sap, or 30 nM saporin-S6 (Sap); ( J ) The cells were treated for 24 h with either vehicle or 1 µM entinostat and then incubated with 30 nM p75IgG-Sap in the absence and in the presence of 100 nM p75IgG added 2 h before the immunotoxin. The values are expressed as percent of control (vehicle) the mean ± SD of four independent experiments. ** p < 0.01, *** p < 0.001 vs. vehicle; ### p < 0.001, ns = not significant by ANOVA followed by Tukey’s test.
Techniques Used: Western Blot, Incubation, Isolation, Expressing, Immunofluorescence, Staining, Microscopy, Luminescence Assay
Figure Legend Snippet: Upregulation of p75NTR and induction of apoptosis by entinostat in neuroblastoma spheroids. ( A , D ) Light microscopy images of IMR-32 and SH-SY5Y multicell spheroids (mcs) that were incubated for 72 h with either vehicle or 1 µM entinostat. The scatter plots report the values of mcs sizes expressed as percent of control (vehicle). Scale bar = 200 µm. ( B , C , E , F ) The spheroids were treated as indicated in ( A , D ) and then analyzed for p75NTR levels and PARP cleavage by Western blot. The values are the mean ± SD of four individual experiments. ** p < 0.01, *** p < 0.001 vs. vehicle by Student’s t -test.
Techniques Used: Light Microscopy, Incubation, Western Blot
Figure Legend Snippet: Expression of p75NTR and histone H3 acetylation in neuroblastoma tumor xenografts and different organs of entinostat-treated mice. ( A , C – F ) Athymic nude mice bearing xenograft of SH-SY5Y cells were treated daily for 10 days with either the vehicle or entinostat (20 mg/kg) by oral gavage. The mice were sacrificed, the tumor xenografts and the indicated organs were resected, and the tissue extracts were analyzed for p75NTR levels by Western blot. ( B ) The levels of acetylated histone H3 were determined in neuroblastoma tumor xenografts of vehicle- and entinostat-treated mice by Western blot. Each lane was loaded with a sample that was obtained from an individual animal (in ( A , B ): four animals that were treated with vehicle and five animals that were treated with entinostat; in ( C – F ), three animals that were treated with either vehicle or entinostat). Scatter plots indicate the absolute values of densitometric ratios and are the mean ± SD of three independent Western blots. * p < 0.05, *** p < 0.001, ns = not significant by Student’s t test.
Techniques Used: Expressing, Western Blot
human p75ntr (Alomone Labs)
Alomone Labs is a verified supplier
Alomone Labs manufactures this product
93
Structured Review
Alomone Labs
human p75ntr
Human P75ntr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human p75ntr/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Human P75ntr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human p75ntr/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
human p75ntr - by Bioz Stars,
2023-09
93/100 stars
Images
1) Product Images from "Sedentary Conditions Promote Subregionally Specific Changes in Brain-Derived Neurotrophic Factor in the Rostral Ventrolateral Medulla"
Article Title: Sedentary Conditions Promote Subregionally Specific Changes in Brain-Derived Neurotrophic Factor in the Rostral Ventrolateral Medulla
Journal: Frontiers in Physiology
doi: 10.3389/fphys.2021.756542
Figure Legend Snippet: Primary antibodies.
Techniques Used: Recombinant
![Expression of Glycosylated (Glyco-p75) and non-Glycosylated (nonGlyco-p75) forms of the p75NTR receptor in the RVLM/RVLM RE following 12weeks of sedentary vs. physically active conditions. (A) Representative Western blot of Glyco-p75 (arrow ~75kDa), nonGlyco-p75 (arrow ~50kDa), and GAPDH expression at different rostrocaudal levels of the RVLM and the RVLM RE . (B) Group data from sedentary (black bars) vs. physically active (white bars) conditions ( n =6 ea) demonstrate no significant overall difference in the expression of Glyco-p75 in sedentary compared to active animals [ F (1, 30)=1.150, p =0.309, main effect of group] and there was also no overall significant effect of rostrocaudal distribution [ F (3,30)=1.877, p =0.155; main effect]. The interaction between experimental groups and rostrocaudal levels did not reach a significance [ F (3,30)=2.029, p =0.131], which precluded further post hoc testing. (C) Group data from sedentary vs. physically active conditions demonstrate a significant interaction term [ F (3,30)=3.384, p =0.031] and revealed a significantly lower expression of nonGlyco-p75 in both RVLM subregions ( ** p =0.004 for FN-480 and p =0.006 for FN-240) and in the FN+240 subregion of the RVLM RE ( ** , p =0.001) of sedentary rats. Sedentary rats showed significantly higher expression of nonGlyco-p75 in the FN+480 of the RVLM RE compared to the FN+240 (##, p =0.012) and both RVLM subregions (##, p =0.004 for FN-240 and p <0.001 for FN-480). Physically active rats exhibited significantly higher expression of nonGlyco-p75 in the FN+240 subregion of RVLM RE compared with the FN-480 of RVLM (##, p =0.006). See for results of all rostrocaudal comparisons within groups. (D) Group data showing that the Glyco-p75/nonGlyco-p75 ratio was overall significantly higher in sedentary rats vs. physically active [*, F (1,30)=20.829, p =0.001, main effect] and that there was an overall significant main effect of rostrocaudal distribution [#, F (3,30)=7.205, p <0.001, main effect]. Simple main effect testing revealed a significantly higher Glyco-p75/nonGlyco-p75 ratio in the most caudal FN-480 subregion of the RVLM compared with two subregions (FN+240 and FN+480) of the RVLM RE ( p =0.009 and p <0.001, respectively, see for all simple main effect comparisons). The interaction between main effects did not reach a significance [ F (3,30)=2.895, p =0.051] which precluded further post hoc testing. Data in (C,D) were log10 transformed in order to achieve normal distribution prior to running two-way mixed ANOVAs.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8431/pmc08548431/pmc08548431__fphys-12-756542-g005.jpg "... Glycosylated (Glyco-p75) and non-Glycosylated (nonGlyco-p75) forms of the p75NTR receptor in the RVLM/RVLM RE following 12weeks of ...")
Figure Legend Snippet: Expression of Glycosylated (Glyco-p75) and non-Glycosylated (nonGlyco-p75) forms of the p75NTR receptor in the RVLM/RVLM RE following 12weeks of sedentary vs. physically active conditions. (A) Representative Western blot of Glyco-p75 (arrow ~75kDa), nonGlyco-p75 (arrow ~50kDa), and GAPDH expression at different rostrocaudal levels of the RVLM and the RVLM RE . (B) Group data from sedentary (black bars) vs. physically active (white bars) conditions ( n =6 ea) demonstrate no significant overall difference in the expression of Glyco-p75 in sedentary compared to active animals [ F (1, 30)=1.150, p =0.309, main effect of group] and there was also no overall significant effect of rostrocaudal distribution [ F (3,30)=1.877, p =0.155; main effect]. The interaction between experimental groups and rostrocaudal levels did not reach a significance [ F (3,30)=2.029, p =0.131], which precluded further post hoc testing. (C) Group data from sedentary vs. physically active conditions demonstrate a significant interaction term [ F (3,30)=3.384, p =0.031] and revealed a significantly lower expression of nonGlyco-p75 in both RVLM subregions ( ** p =0.004 for FN-480 and p =0.006 for FN-240) and in the FN+240 subregion of the RVLM RE ( ** , p =0.001) of sedentary rats. Sedentary rats showed significantly higher expression of nonGlyco-p75 in the FN+480 of the RVLM RE compared to the FN+240 (##, p =0.012) and both RVLM subregions (##, p =0.004 for FN-240 and p <0.001 for FN-480). Physically active rats exhibited significantly higher expression of nonGlyco-p75 in the FN+240 subregion of RVLM RE compared with the FN-480 of RVLM (##, p =0.006). See for results of all rostrocaudal comparisons within groups. (D) Group data showing that the Glyco-p75/nonGlyco-p75 ratio was overall significantly higher in sedentary rats vs. physically active [*, F (1,30)=20.829, p =0.001, main effect] and that there was an overall significant main effect of rostrocaudal distribution [#, F (3,30)=7.205, p <0.001, main effect]. Simple main effect testing revealed a significantly higher Glyco-p75/nonGlyco-p75 ratio in the most caudal FN-480 subregion of the RVLM compared with two subregions (FN+240 and FN+480) of the RVLM RE ( p =0.009 and p <0.001, respectively, see for all simple main effect comparisons). The interaction between main effects did not reach a significance [ F (3,30)=2.895, p =0.051] which precluded further post hoc testing. Data in (C,D) were log10 transformed in order to achieve normal distribution prior to running two-way mixed ANOVAs.
Techniques Used: Expressing, Western Blot, Transformation Assay
p75ntr (Alomone Labs)
Alomone Labs is a verified supplier
Alomone Labs manufactures this product
93
Structured Review
Alomone Labs
p75ntr
P75ntr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p75ntr/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
P75ntr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p75ntr/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
p75ntr - by Bioz Stars,
2023-09
93/100 stars
Images
1) Product Images from "Sedentary Conditions Promote Subregionally Specific Changes in Brain-Derived Neurotrophic Factor in the Rostral Ventrolateral Medulla"
Article Title: Sedentary Conditions Promote Subregionally Specific Changes in Brain-Derived Neurotrophic Factor in the Rostral Ventrolateral Medulla
Journal: Frontiers in Physiology
doi: 10.3389/fphys.2021.756542
Figure Legend Snippet: Primary antibodies.
Techniques Used: Recombinant
Figure Legend Snippet: Expression of Glycosylated (Glyco-p75) and non-Glycosylated (nonGlyco-p75) forms of the p75NTR receptor in the RVLM/RVLM RE following 12weeks of sedentary vs. physically active conditions. (A) Representative Western blot of Glyco-p75 (arrow ~75kDa), nonGlyco-p75 (arrow ~50kDa), and GAPDH expression at different rostrocaudal levels of the RVLM and the RVLM RE . (B) Group data from sedentary (black bars) vs. physically active (white bars) conditions ( n =6 ea) demonstrate no significant overall difference in the expression of Glyco-p75 in sedentary compared to active animals [ F (1, 30)=1.150, p =0.309, main effect of group] and there was also no overall significant effect of rostrocaudal distribution [ F (3,30)=1.877, p =0.155; main effect]. The interaction between experimental groups and rostrocaudal levels did not reach a significance [ F (3,30)=2.029, p =0.131], which precluded further post hoc testing. (C) Group data from sedentary vs. physically active conditions demonstrate a significant interaction term [ F (3,30)=3.384, p =0.031] and revealed a significantly lower expression of nonGlyco-p75 in both RVLM subregions ( ** p =0.004 for FN-480 and p =0.006 for FN-240) and in the FN+240 subregion of the RVLM RE ( ** , p =0.001) of sedentary rats. Sedentary rats showed significantly higher expression of nonGlyco-p75 in the FN+480 of the RVLM RE compared to the FN+240 (##, p =0.012) and both RVLM subregions (##, p =0.004 for FN-240 and p <0.001 for FN-480). Physically active rats exhibited significantly higher expression of nonGlyco-p75 in the FN+240 subregion of RVLM RE compared with the FN-480 of RVLM (##, p =0.006). See for results of all rostrocaudal comparisons within groups. (D) Group data showing that the Glyco-p75/nonGlyco-p75 ratio was overall significantly higher in sedentary rats vs. physically active [*, F (1,30)=20.829, p =0.001, main effect] and that there was an overall significant main effect of rostrocaudal distribution [#, F (3,30)=7.205, p <0.001, main effect]. Simple main effect testing revealed a significantly higher Glyco-p75/nonGlyco-p75 ratio in the most caudal FN-480 subregion of the RVLM compared with two subregions (FN+240 and FN+480) of the RVLM RE ( p =0.009 and p <0.001, respectively, see for all simple main effect comparisons). The interaction between main effects did not reach a significance [ F (3,30)=2.895, p =0.051] which precluded further post hoc testing. Data in (C,D) were log10 transformed in order to achieve normal distribution prior to running two-way mixed ANOVAs.
Techniques Used: Expressing, Western Blot, Transformation Assay
ant 007 ag (Alomone Labs)
Alomone Labs is a verified supplier
Alomone Labs manufactures this product
93
Structured Review
Alomone Labs
ant 007 ag
Ant 007 Ag, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ant 007 ag/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Ant 007 Ag, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ant 007 ag/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ant 007 ag - by Bioz Stars,
2023-09
93/100 stars
Images
rabbit anti p75ntr atto 488 (Alomone Labs)
Alomone Labs is a verified supplier
Alomone Labs manufactures this product
93
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Alomone Labs
rabbit anti p75ntr atto 488
Rabbit Anti P75ntr Atto 488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti p75ntr atto 488/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Rabbit Anti P75ntr Atto 488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti p75ntr atto 488/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti p75ntr atto 488 - by Bioz Stars,
2023-09
93/100 stars
Images
1) Product Images from "An iPSC model of hereditary sensory neuropathy-1 reveals L-serine-responsive deficits in neuronal ganglioside composition and axoglial interactions"
Article Title: An iPSC model of hereditary sensory neuropathy-1 reveals L-serine-responsive deficits in neuronal ganglioside composition and axoglial interactions
Journal: Cell Reports Medicine
doi: 10.1016/j.xcrm.2021.100345
Figure Legend Snippet: Neurotrophic signaling is impaired in HSN1 neurons (A) Photomicrographs of 8-week-old iPSCdSNs immunocytochemically stained for NF200 (green) and pERM (red) at baseline, after neurotrophic stimulation and after neurotrophic starvation. (B) Quantification of pERM membrane fluorescence intensity determined by profile plot analysis across multiple differentiations. Between groups comparison, ∗p ≤ 0.0001 by 2-way ANOVA (F = 31.51, df = 5). Individual comparisons by Tukey’s post hoc tests: healthy control baseline versus HSN1 baseline, ∗p = 0.0001; healthy control stimulated versus HSN1 stimulated, ∗p ≤ 0.0001; healthy control starved versus HSN1 starved, p = 0.939; and healthy control baseline versus healthy control starved, ∗p = < 0.0001. Each data point represents the mean from an independent differentiation and iPSC line (three iPSC lines analyzed across two differentiations per genotype). (C) Differential interference contrast (DIC) brightfield images of live control and HSN1 iPSCdSNs incubated with a fluorescently tagged anti-p75NTR antibody. (D) Quantification of p75NTR membrane fluorescence intensity determined by profile plot analysis across multiple differentiations. Control versus HSN1 comparison, ∗p = 0.0003 by 2-way ANOVA (F = 38.25, df = 1). Each data point represents the mean from an independent differentiation and iPSC line (three iPSC lines analyzed across two differentiations per genotype). (E) Western blots of 8-week-old iPSCdSNs for ERK and p-ERK with (+NT) and without (−NT) neurotrophic stimulation. (F) Normalized relative intensity of western blots expressed as p-ERK/ERK ratio for each control and HSN1 line, and mean averages (−NT, ∗p = 0.0166 and +NT, ∗p = 0.0008 by Student’s t test). Colors on graphs represent different iPSC lines and all error bars are SEM. All images within a panel are taken at the same magnification.
Techniques Used: Staining, Fluorescence, Incubation, Western Blot
Figure Legend Snippet:
Techniques Used: Labeling, Recombinant, Expressing, Software
anti p75ntr antibody (Alomone Labs)
Alomone Labs is a verified supplier
Alomone Labs manufactures this product
93
Structured Review
Alomone Labs
anti p75ntr antibody
Anti P75ntr Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p75ntr antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Anti P75ntr Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p75ntr antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti p75ntr antibody - by Bioz Stars,
2023-09
93/100 stars
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p75 (Alomone Labs)
Alomone Labs is a verified supplier
Alomone Labs manufactures this product
94
Structured Review
Alomone Labs
p75
P75, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p75/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
P75, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p75/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
p75 - by Bioz Stars,
2023-09
94/100 stars
Images
1) Product Images from "Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury"
Article Title: Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury
Journal: The Journal of Clinical Investigation
doi: 10.1172/JCI97837
Figure Legend Snippet: (A) Uroplakin-positive (UP+) umbrella cells are the major cell types that undergo apoptosis at 7 hours after SCI. The bladders were processed for TUNEL reaction and subsequently stained with UP or p75 antibodies. Urothelial and muscle layers of the bladder wall are indicated by U and M, respectively, and the bladder lumen is indicated by L. Scale bar: 150 μm. (B) p75 is responsible for umbrella cell apoptosis after SCI as indicated by the lack of TUNEL+ cells in the p75KO. The data were analyzed using repeated measures of 2-way ANOVA after Greenhouse-Geisser correction, which resulted in P < 0.001 comparing the vehicle and LM11A-31 treatments and the WT and p75-null mice. Subsequent pairwise comparisons were made using Bonferroni correction. (C) Intravesical instillation of proNGF blocking antibody blocked umbrella cell apoptosis completely at 7 hours after injury. The data were analyzed by Student’s t tests.
Techniques Used: TUNEL Assay, Staining, Blocking Assay
Figure Legend Snippet: (A) Diagram of the targeting vectors in p75Δ-UP3a and p75c-UP3a mice. Red triangles represent loxP sites, and green P1 and P2 arrows represent PCR primers used in B. (B) Selective deletion of p75 in urothelial cells. Urothelial cells from tamoxifen-treated p75Δ-UP3a and p75c-UP3a mice were scraped into a tube, and the isolated genomic DNA was subjected to PCR using P1 and P2 primers. The primers generate PCR product only when Cre is activated. Note that the PCR band is present only in umbrella cells and not in the bladder minus urothelium in p75Δ-UP3a mice, indicating a selective p75 deletion in the urothelium. (C) p75 is not detected in umbrella cells (U), while it is clearly present in the muscle (M). L, bladder lumen. Scale bar: 150 μm. (D) Umbrella cell apoptosis was completely blocked in p75Δ-UP3a compared with that in p75c-UP3a mice.
Techniques Used: Isolation
Figure Legend Snippet: (A) Representative recordings of intravesical pressure during continuous intravesical infusion of saline and an open urethral outlet in conscious, unrestrained p75c-UP3a and p75Δ-UP3a mice with no SCI and SCI (2 weeks). (B) Overall voiding efficiency in no-SCI p75Δ-UP3a and p75c-UP3a mice was similar, although after SCI, p75Δ-UP3a mice exhibited worse bladder function than p75c-UP3a mice with a 32% drop in voiding efficiency (n = 3–5). (C and D) Under no-SCI condition, p75Δ-UP3a mice had significantly decreased infused volumes (IVs) that induced micturition and intermicturition intervals compared with those observed in p75c-UP3a mice. SCI significantly reduced intermicturition intervals (C) and IVs (D) in p75c-UP3a mice, and these effects were reversed in p75Δ-UP3a mice. Note that left y axes represent no SCI, while right y axes represent SCI groups. (E) No-SCI p75Δ-UP3a mice exhibited significantly increased average, minimum, and threshold bladder pressures compared with control, with no change in maximum micturition pressure. Comparisons among groups were made using ANOVA. When F ratios exceeded the adjusted critical value (P ≤ 0.0125), Bonferroni’s multiple-comparisons test was used to compare means among groups. (F) ProNGF/p75 signaling negatively influences TrpV4-mediated Ca2+ flux in primary mouse urothelial cells. Urothelial cells were incubated with 10 ng/ml of proNGF or vehicle for 30 minutes before Fluo-4 AM loading and GSK1016790A addition at 50 nM (red arrows; n = 4 independent experiments). (G) Quantification of the average peak amplitude of ΔF/F. Comparisons among groups were made using ANOVA, and pairwise comparisons were made by Tukey’s multiple-comparisons test (3–8 cells were included per treatment). (H) GSK1016790A facilitates cell-surface targeting of TrpV4 in HEK293T cells, which is inhibited by proNGF. ProNGF-mediated inhibition is lifted with LM11A-31 preincubation.
Techniques Used: Incubation, Inhibition
Figure Legend Snippet: (A) LM11A-31 reduced the number of c-fos+ cells in L6 spinal cords after SCI. DCM, dorsal commissure; cc, central canal; MDH, medial dorsal horn. Scale bar: 150 μm. (B) Quantification of A. Comparisons among groups were made using 1-way ANOVA (P ≤ 0.001), and pairwise comparisons were made by Tukey’s multiple-comparisons test. (C) p75 is expressed among tyrosine hydroxylase–positive (TH+) sensory neurons in L6/S1 DRG. DRGs were processed for double immunohistochemistry for TH and p75. Scale bars: 150 μm. The boxed areas are shown in the far right column; scale bars: 37.5 μm. (D) Colocalization of TH and p75 in fibers and neuronal soma in the DCM of L6/S1 spinal cords. Scale bars: 150 μm. The boxed areas are shown in the far right column; scale bars: 37.5 μm. Experiments were performed 3 times, and representative ones are shown.
Techniques Used: Immunohistochemistry
rabbit anti p75 nerve growth factor ngf receptor (Alomone Labs)
Alomone Labs is a verified supplier
Alomone Labs manufactures this product
93
Structured Review
Alomone Labs
rabbit anti p75 nerve growth factor ngf receptor
Rabbit Anti P75 Nerve Growth Factor Ngf Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti p75 nerve growth factor ngf receptor/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Rabbit Anti P75 Nerve Growth Factor Ngf Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti p75 nerve growth factor ngf receptor/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti p75 nerve growth factor ngf receptor - by Bioz Stars,
2023-09
93/100 stars
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anti p75ntr rabbit polyclonal antibodies (Alomone Labs)
Alomone Labs is a verified supplier
Alomone Labs manufactures this product
93
Structured Review
Alomone Labs
anti p75ntr rabbit polyclonal antibodies
Anti P75ntr Rabbit Polyclonal Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p75ntr rabbit polyclonal antibodies/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Anti P75ntr Rabbit Polyclonal Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p75ntr rabbit polyclonal antibodies/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti p75ntr rabbit polyclonal antibodies - by Bioz Stars,
2023-09
93/100 stars
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anti cd271 (Alomone Labs)
Alomone Labs is a verified supplier
Alomone Labs manufactures this product
93
Structured Review
Alomone Labs
anti cd271
Anti Cd271, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd271/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Anti Cd271, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd271/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti cd271 - by Bioz Stars,
2023-09
93/100 stars
Images
1) Product Images from "Notch3 Signaling-Mediated Melanoma-Endothelial Crosstalk Regulates Melanoma Stem-Like Cell Homeostasis and Niche Morphogenesis"
Article Title: Notch3 Signaling-Mediated Melanoma-Endothelial Crosstalk Regulates Melanoma Stem-Like Cell Homeostasis and Niche Morphogenesis
Journal: Laboratory investigation; a journal of technical methods and pathology
doi: 10.1038/labinvest.2017.1
Figure Legend Snippet: Two dimensional (2D) melanoma-EC co-culture model recapitulates MSLC niche in vitro . A. Schematic representation of the 2D MSLC niche model in vitro . GFP-labeled CD133 − non-MSLCs were co-cultured with RFP-labeled human umbilical vein endothelial cells (HUVECs) at 1:1 or 1:4 ratios for 5 days. B. In this model, ECs aligned to form branching tubular networks, reminiscent of the vascular niche in vivo (Magnification, ×100; scale bar, 200 μm). Co-cultured melanoma cells were then segregated from ECs by flow cytometry. C. MSLC (e.g., CD133 and CD271) and VM (e.g., CD144) markers were up-regulated in co-cultured melanoma cells compared to their mono-culture counter parts using qRT-PCR, simulating “dynamic stemness” and VM morphogenesis in vitro . D. Such a niche-inducing phenomenon was most pronounced when melanoma-EC contact is permitted, while EC extracellular matrix (ECM) or soluble factors in transwell cultures alone exhibited limited/partial effects. *, P < 0.05.
Techniques Used: Co-Culture Assay, In Vitro, Labeling, Cell Culture, In Vivo, Flow Cytometry, Quantitative RT-PCR
Figure Legend Snippet: Effective and functional Notch3 KD depletes MSLCs and VM-engaging melanoma cells in 1205 Lu xenografts. A. Western blot analyses demonstrated that stem cell markers (e.g., CD133 and CD271) as well as VM maker, CD144, were down-regulated in Notch3 KD 1205 Lu tumors, compared to non-target control (A). Consistent with the observed VM inhibition by Notch3 KD above, the expression of Tie-1, a VM-associated gene, was also down-regulated in Nocth3 KD 1205Lu xenografts by qRT-PCR (B). Of note, the efficacy of Notch3 KD was maintained in the tumor xenografts as shown by Western blotting (A). On the other hand, in A375 melanoma cells where no change in tumorigenicity was appreciated following Notch3 KD, the expression of stem-like cell marker, CD271, was not affected (C). Attempts to further validate CD133 and CD144 in Notch3 KD A375 xenografts using Western blot analysis (C8161 lysate included as a positive control) were unsuccessful due to sensitivity issue ( ; note that CD133 and CD144 signals were undetectable both in the control and KD xenografts despite maximal loading and prolonged overnight exposure). *, P < 0.05.
Techniques Used: Functional Assay, Western Blot, Inhibition, Expressing, Quantitative RT-PCR, Marker, Positive Control