Anti P75ntr Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Novel Technique for Retinal Nerve Cell Regeneration with Electrophysiological Functions Using Human Iris-Derived iPS Cells"
Article Title: Novel Technique for Retinal Nerve Cell Regeneration with Electrophysiological Functions Using Human Iris-Derived iPS Cells
Figure Legend Snippet: Process of differentiation of H-iris iPS cells to nervous system cells. Cells were pre-differentiated from ( a ) H-iris iPS cells to ( b ) neural stem/progenitor cells by adhesive culture. ( c ) Cells sorted with p75NTR were differentiated into neurons. By changing the medium condition, the nerve cells were differentiated into Recoverin-positive cells. ( d ) In contrast, after suspension culture, neurites were elongated by adhesive culture and differentiated into retinal ganglion cells. Bars: 100 μm.
Figure Legend Snippet: Cells sorted with p75NTR can concentrate Recoverin-positive cells. ( a ) Before p75NTR sorting, cells with large cytoplasms ( arrowheads ) were mixed. ( b ) Fixed cells were analyzed for p75NTR and Nestin by FCM. Most of the cells sorted in the strong positive region of p75NTR were double-positive for ( c ) p75NTR and ( d ) Nestin. ( e , f ) The morphology of cells sorted by their strong positivity for ( e ) p75NTR or ( f ) their weak positivity or negativity for p75NTR. ( g ) Relative semi-quantitative analysis of Recoverin gene expression in cells selected by p75NTR, and pre/post-differentiated cells. ( h ) Fluorescent immunostaining of Recoverin in differentiated cells. Bar in panel ( a ): 50 μm, bars in panels ( c , d ): 20 μm, bars in panels ( e , f , h ): 100 μm.
Techniques Used: Expressing, Immunostaining
Figure Legend Snippet: p75NTR-positive cells observed in human iris tissue and cultured cells: ( a ) HE-stained human iris tissue; * The lens side of the iris. ( b ) Differential interference contrast image of iris tissue. ( c ) Fluorescent immunostaining of p75NTR; arrowheads = p75NTR-positive cells. ( d ) Iris-derived cells were cultured for 10 days; the inset shows cells growing from around the pigmented cells on the third day of culture. ( e ) p75NTR-positive cells (region of Gate 1, G1) were isolated with a cell sorter; the blue line represents the histogram of negative cells. ( f ) Morphology of p75NTR-positive sorted cells. Bars in panels ( a – c ), 50 μm; bars in panel ( d )’s inset and panel ( f ), 100 μm; bar in panel ( d ), 200 μm.
Techniques Used: Cell Culture, Staining, Immunostaining, Derivative Assay, Isolation