prongf  (Alomone Labs)


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    Structured Review

    Alomone Labs prongf
    ( A – G ) Biochemical analysis of NGF, TrkA and p75 NTR in the retina from 1 to 14 days after crush ( n = 6 per group). Crush retinas had increased levels of NGF anylate measured by Elisa ( A ) and <t>proNGF</t> quantified by WB analysis ( B ) at 7 d and 14 d. TrkA expression was reduced in Crush retinas at 14 d ( C ) while p75 NTR expression was increased in Crush retinas at all time points analyzed ( D ). Representative images of the WB of the time course in CoEye ( E ) and crushed retina ( F ). ( G ) The specificity of proNGF signal detected in both brain and retinal lysates using anti-proNGF <t>by</t> <t>Alomone</t> (Lanes 1 and 4). No specific signal was found in naïve samples (non reduced and denatured; Lanes 2 and 5), and by pre-incubation with the control peptide provided by Alomone (Lanes 3 and 6). * p
    Prongf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prongf/product/Alomone Labs
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    prongf - by Bioz Stars, 2022-12
    94/100 stars

    Images

    1) Product Images from "Time-Dependent Nerve Growth Factor Signaling Changes in the Rat Retina During Optic Nerve Crush-Induced Degeneration of Retinal Ganglion Cells"

    Article Title: Time-Dependent Nerve Growth Factor Signaling Changes in the Rat Retina During Optic Nerve Crush-Induced Degeneration of Retinal Ganglion Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms18010098

    ( A – G ) Biochemical analysis of NGF, TrkA and p75 NTR in the retina from 1 to 14 days after crush ( n = 6 per group). Crush retinas had increased levels of NGF anylate measured by Elisa ( A ) and proNGF quantified by WB analysis ( B ) at 7 d and 14 d. TrkA expression was reduced in Crush retinas at 14 d ( C ) while p75 NTR expression was increased in Crush retinas at all time points analyzed ( D ). Representative images of the WB of the time course in CoEye ( E ) and crushed retina ( F ). ( G ) The specificity of proNGF signal detected in both brain and retinal lysates using anti-proNGF by Alomone (Lanes 1 and 4). No specific signal was found in naïve samples (non reduced and denatured; Lanes 2 and 5), and by pre-incubation with the control peptide provided by Alomone (Lanes 3 and 6). * p
    Figure Legend Snippet: ( A – G ) Biochemical analysis of NGF, TrkA and p75 NTR in the retina from 1 to 14 days after crush ( n = 6 per group). Crush retinas had increased levels of NGF anylate measured by Elisa ( A ) and proNGF quantified by WB analysis ( B ) at 7 d and 14 d. TrkA expression was reduced in Crush retinas at 14 d ( C ) while p75 NTR expression was increased in Crush retinas at all time points analyzed ( D ). Representative images of the WB of the time course in CoEye ( E ) and crushed retina ( F ). ( G ) The specificity of proNGF signal detected in both brain and retinal lysates using anti-proNGF by Alomone (Lanes 1 and 4). No specific signal was found in naïve samples (non reduced and denatured; Lanes 2 and 5), and by pre-incubation with the control peptide provided by Alomone (Lanes 3 and 6). * p

    Techniques Used: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Incubation

    2) Product Images from "Time-Dependent Nerve Growth Factor Signaling Changes in the Rat Retina During Optic Nerve Crush-Induced Degeneration of Retinal Ganglion Cells"

    Article Title: Time-Dependent Nerve Growth Factor Signaling Changes in the Rat Retina During Optic Nerve Crush-Induced Degeneration of Retinal Ganglion Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms18010098

    ( A – G ) Biochemical analysis of NGF, TrkA and p75 NTR in the retina from 1 to 14 days after crush ( n = 6 per group). Crush retinas had increased levels of NGF anylate measured by Elisa ( A ) and proNGF quantified by WB analysis ( B ) at 7 d and 14 d. TrkA expression was reduced in Crush retinas at 14 d ( C ) while p75 NTR expression was increased in Crush retinas at all time points analyzed ( D ). Representative images of the WB of the time course in CoEye ( E ) and crushed retina ( F ). ( G ) The specificity of proNGF signal detected in both brain and retinal lysates using anti-proNGF by Alomone (Lanes 1 and 4). No specific signal was found in naïve samples (non reduced and denatured; Lanes 2 and 5), and by pre-incubation with the control peptide provided by Alomone (Lanes 3 and 6). * p
    Figure Legend Snippet: ( A – G ) Biochemical analysis of NGF, TrkA and p75 NTR in the retina from 1 to 14 days after crush ( n = 6 per group). Crush retinas had increased levels of NGF anylate measured by Elisa ( A ) and proNGF quantified by WB analysis ( B ) at 7 d and 14 d. TrkA expression was reduced in Crush retinas at 14 d ( C ) while p75 NTR expression was increased in Crush retinas at all time points analyzed ( D ). Representative images of the WB of the time course in CoEye ( E ) and crushed retina ( F ). ( G ) The specificity of proNGF signal detected in both brain and retinal lysates using anti-proNGF by Alomone (Lanes 1 and 4). No specific signal was found in naïve samples (non reduced and denatured; Lanes 2 and 5), and by pre-incubation with the control peptide provided by Alomone (Lanes 3 and 6). * p

    Techniques Used: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Incubation

    3) Product Images from "Electroporation-mediated gene delivery of cleavage-resistant pro-nerve growth factor causes retinal neuro- and vascular degeneration"

    Article Title: Electroporation-mediated gene delivery of cleavage-resistant pro-nerve growth factor causes retinal neuro- and vascular degeneration

    Journal: Molecular Vision

    doi:

    Overexpression of proNGF reduced NGF and induced expression of TrkA and p75 NTR , but not sortilin. A , B : Western blot (WB) analysis of rat retinal lysate showed significant (1.5-fold) increase in expression of TrkA and p75 NTR in rats electroporated with pGFP-proNGF123 as compared with those electroporated with pGFP (n=4). C : WB analysis showed that overexpression of pGFP-proNGF123 in rat retinas did not affect sortilin protein expression level as compared with the control rats (n=4). D : WB analysis showed that overexpression of pGFP-proNGF123 significantly reduced NGF expression (n=5) compared with GFP controls. The asterisk represents significant difference as compared with control group at p
    Figure Legend Snippet: Overexpression of proNGF reduced NGF and induced expression of TrkA and p75 NTR , but not sortilin. A , B : Western blot (WB) analysis of rat retinal lysate showed significant (1.5-fold) increase in expression of TrkA and p75 NTR in rats electroporated with pGFP-proNGF123 as compared with those electroporated with pGFP (n=4). C : WB analysis showed that overexpression of pGFP-proNGF123 in rat retinas did not affect sortilin protein expression level as compared with the control rats (n=4). D : WB analysis showed that overexpression of pGFP-proNGF123 significantly reduced NGF expression (n=5) compared with GFP controls. The asterisk represents significant difference as compared with control group at p

    Techniques Used: Over Expression, Expressing, Western Blot

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    Alomone Labs anti prongf
    <t>proNGF</t> expression in nevi, primary melanoma and metastatic human tissues ( A – F ). Immunohistochemical detection of proNGF, representative pictures are shown for compound nevi ( A ), dysplastic nevi ( B ), thin primary melanomas ( C ), thick primary melanomas ( D ), lymph node metastases ( E ) and distant metastases ( F ). ( G ) Digital quantification of proNGF staining intensities according to grouped pathological subtypes: nevi (h-score = 156.2, IQR 138.9–176.1), primary melanomas (h-score = 129.0, IQR 111.8–1148.1) and metastases (h-score = 115.1, IQR 93.33–130.1). ( H ) proNGF staining intensities for individual pathological subtypes: CN = compound nevi (h-score = 143.4, IQR 126.1–176.0), DN = dysplastic nevi (h-score = 158.6, IQR 143.0–189.6), TnP = thin primary (h-score = 125.3, IQR 111.8–134.6), TkP = thick primary (h-score = 133.6, IQR 109.2–154.1), LNM = lymph node metastasis (h-score = 116.7, IQR 105.9–132.3), DM = distant metastasis (h-score = 101.8, IQR 91.64–129.7). ( I ) Correlation of proNGF and NGF stain intensities. Scale bar = 90 µm. Data are expressed as medians (horizontal line in the center of the box) and box limits indicate the interquartile range (IQR) with the whiskers extending 1.5 times the IQR from the 25th and 75th percentiles; outliers are represented by dots. H-score distributions were compared using the Wilcoxon rank-sum (dichotomous) or Kruskal–Wallis (multiple comparisons) tests ( p
    Anti Prongf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti prongf/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    proNGF expression in nevi, primary melanoma and metastatic human tissues ( A – F ). Immunohistochemical detection of proNGF, representative pictures are shown for compound nevi ( A ), dysplastic nevi ( B ), thin primary melanomas ( C ), thick primary melanomas ( D ), lymph node metastases ( E ) and distant metastases ( F ). ( G ) Digital quantification of proNGF staining intensities according to grouped pathological subtypes: nevi (h-score = 156.2, IQR 138.9–176.1), primary melanomas (h-score = 129.0, IQR 111.8–1148.1) and metastases (h-score = 115.1, IQR 93.33–130.1). ( H ) proNGF staining intensities for individual pathological subtypes: CN = compound nevi (h-score = 143.4, IQR 126.1–176.0), DN = dysplastic nevi (h-score = 158.6, IQR 143.0–189.6), TnP = thin primary (h-score = 125.3, IQR 111.8–134.6), TkP = thick primary (h-score = 133.6, IQR 109.2–154.1), LNM = lymph node metastasis (h-score = 116.7, IQR 105.9–132.3), DM = distant metastasis (h-score = 101.8, IQR 91.64–129.7). ( I ) Correlation of proNGF and NGF stain intensities. Scale bar = 90 µm. Data are expressed as medians (horizontal line in the center of the box) and box limits indicate the interquartile range (IQR) with the whiskers extending 1.5 times the IQR from the 25th and 75th percentiles; outliers are represented by dots. H-score distributions were compared using the Wilcoxon rank-sum (dichotomous) or Kruskal–Wallis (multiple comparisons) tests ( p

    Journal: International Journal of Molecular Sciences

    Article Title: Expression of NGF/proNGF and Their Receptors TrkA, p75NTR and Sortilin in Melanoma

    doi: 10.3390/ijms23084260

    Figure Lengend Snippet: proNGF expression in nevi, primary melanoma and metastatic human tissues ( A – F ). Immunohistochemical detection of proNGF, representative pictures are shown for compound nevi ( A ), dysplastic nevi ( B ), thin primary melanomas ( C ), thick primary melanomas ( D ), lymph node metastases ( E ) and distant metastases ( F ). ( G ) Digital quantification of proNGF staining intensities according to grouped pathological subtypes: nevi (h-score = 156.2, IQR 138.9–176.1), primary melanomas (h-score = 129.0, IQR 111.8–1148.1) and metastases (h-score = 115.1, IQR 93.33–130.1). ( H ) proNGF staining intensities for individual pathological subtypes: CN = compound nevi (h-score = 143.4, IQR 126.1–176.0), DN = dysplastic nevi (h-score = 158.6, IQR 143.0–189.6), TnP = thin primary (h-score = 125.3, IQR 111.8–134.6), TkP = thick primary (h-score = 133.6, IQR 109.2–154.1), LNM = lymph node metastasis (h-score = 116.7, IQR 105.9–132.3), DM = distant metastasis (h-score = 101.8, IQR 91.64–129.7). ( I ) Correlation of proNGF and NGF stain intensities. Scale bar = 90 µm. Data are expressed as medians (horizontal line in the center of the box) and box limits indicate the interquartile range (IQR) with the whiskers extending 1.5 times the IQR from the 25th and 75th percentiles; outliers are represented by dots. H-score distributions were compared using the Wilcoxon rank-sum (dichotomous) or Kruskal–Wallis (multiple comparisons) tests ( p

    Article Snippet: The following primary antibodies were applied: anti-proNGF (0.8 µg/mL, catalogue number ANT005, Alomone labs, Jerusalem, Israel), anti-NGF (13.3 µg/mL, catalogue number ab52918, Abcam, VIC, Australia), anti-sortilin (0.8 µg/mL, catalogue number ANT009, Alomone labs, Jerusalem, Israel), anti-p75NTR (2 µg/mL, catalogue number ANT007, Alomone labs, Jerusalem, Israel), anti-TrkA (1:200 dilution, catalogue number cs-2508, Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Expressing, Immunohistochemistry, Staining

    Knockdown of astrocytic Grin2a inhibits the increase of β‐NGF induced by Aβ. (a) Representative western blot band and quantification showing the effects of astrocytic Grin2a knockdown on β‐NGF precursor, proNGF level. (b) Representative western blot band and quantification showing the effects of astrocytic Grin2a knockdown on β‐NGF level. (c) Scheme diagram of confocal images analysis of β‐NGF in astrocytes (GFAP positive) (the upper) and representative quantification in CA3 subregion of the hippocampus (the lower). Bar, 200 μm. d. Quantification of β‐NGF immunofluorescent intensity in the rat hippocampus. n = 13–15 sections from 3 rats/group.*vs. vehicle, * p

    Journal: Aging Cell

    Article Title: Knockdown of astrocytic Grin2a aggravates β‐amyloid‐induced memory and cognitive deficits through regulating nerve growth factor). Knockdown of astrocytic Grin2a aggravates β‐amyloid‐induced memory and cognitive deficits through regulating nerve growth factor

    doi: 10.1111/acel.13437

    Figure Lengend Snippet: Knockdown of astrocytic Grin2a inhibits the increase of β‐NGF induced by Aβ. (a) Representative western blot band and quantification showing the effects of astrocytic Grin2a knockdown on β‐NGF precursor, proNGF level. (b) Representative western blot band and quantification showing the effects of astrocytic Grin2a knockdown on β‐NGF level. (c) Scheme diagram of confocal images analysis of β‐NGF in astrocytes (GFAP positive) (the upper) and representative quantification in CA3 subregion of the hippocampus (the lower). Bar, 200 μm. d. Quantification of β‐NGF immunofluorescent intensity in the rat hippocampus. n = 13–15 sections from 3 rats/group.*vs. vehicle, * p

    Article Snippet: Membranes were then blocked with 5% skim milk in Tris buffered saline tween 20 (TBST) buffer before overnight incubation at 4℃ with the following primary antibodies: mouse anti‐PSD95 (1:1000, MA1‐045, Thermo Fisher), anti‐GAPDH (1:1000, sc‐32233; Santa Cruz), β‐actin (1:3000, SAB1305554, Sigma); rabbit anti‐GluN2A (1:1000, NBP2‐19551, Novus Biologicals), anti‐furin (1:1000, ab183495; Abcam), anti‐SNAP23 (1:1000, 10825‐1‐AP; proteintech), anti‐VAMP3 (1:1000, 10702‐1‐AP; Proteintech), anti‐p‐NF‐κB (1:1000, 3033; CST), anti‐p‐CREB (1:1000, 9198; CST), anti‐p‐ERK (1:1000, 4370; CST), anti‐synaptophysin (1:1000, 36406; CST), anti‐proNGF (1:1000, ANT‐005, Alomone labs); goat anti‐β‐NGF (1:2000, AF‐556‐NA; R & D Systems), followed by incubation with goat anti‐mouse or anti‐rabbit IgG (H+L)‐HRP, or rabbit anti‐goat IgG (H+L)‐HRP (1:5000, Absin) in TBST.

    Techniques: Western Blot