Prongf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Electroporation-mediated gene delivery of cleavage-resistant pro-nerve growth factor causes retinal neuro- and vascular degeneration"
Article Title: Electroporation-mediated gene delivery of cleavage-resistant pro-nerve growth factor causes retinal neuro- and vascular degeneration
Journal: Molecular Vision
Figure Legend Snippet: Overexpression of proNGF reduced NGF and induced expression of TrkA and p75 NTR , but not sortilin. A , B : Western blot (WB) analysis of rat retinal lysate showed significant (1.5-fold) increase in expression of TrkA and p75 NTR in rats electroporated with pGFP-proNGF123 as compared with those electroporated with pGFP (n=4). C : WB analysis showed that overexpression of pGFP-proNGF123 in rat retinas did not affect sortilin protein expression level as compared with the control rats (n=4). D : WB analysis showed that overexpression of pGFP-proNGF123 significantly reduced NGF expression (n=5) compared with GFP controls. The asterisk represents significant difference as compared with control group at p
Techniques Used: Over Expression, Expressing, Western Blot
2) Product Images from "Time-Dependent Nerve Growth Factor Signaling Changes in the Rat Retina During Optic Nerve Crush-Induced Degeneration of Retinal Ganglion Cells"
Article Title: Time-Dependent Nerve Growth Factor Signaling Changes in the Rat Retina During Optic Nerve Crush-Induced Degeneration of Retinal Ganglion Cells
Journal: International Journal of Molecular Sciences
Figure Legend Snippet: ( A – G ) Biochemical analysis of NGF, TrkA and p75 NTR in the retina from 1 to 14 days after crush ( n = 6 per group). Crush retinas had increased levels of NGF anylate measured by Elisa ( A ) and proNGF quantified by WB analysis ( B ) at 7 d and 14 d. TrkA expression was reduced in Crush retinas at 14 d ( C ) while p75 NTR expression was increased in Crush retinas at all time points analyzed ( D ). Representative images of the WB of the time course in CoEye ( E ) and crushed retina ( F ). ( G ) The specificity of proNGF signal detected in both brain and retinal lysates using anti-proNGF by Alomone (Lanes 1 and 4). No specific signal was found in naïve samples (non reduced and denatured; Lanes 2 and 5), and by pre-incubation with the control peptide provided by Alomone (Lanes 3 and 6). * p
Techniques Used: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Incubation
3) Product Images from "Minocycline Alleviates Death of Oligodendrocytes by Inhibiting Pro-Nerve Growth Factor Production in Microglia after Spinal Cord Injury"
Article Title: Minocycline Alleviates Death of Oligodendrocytes by Inhibiting Pro-Nerve Growth Factor Production in Microglia after Spinal Cord Injury
Journal: The Journal of Neuroscience
Figure Legend Snippet: Minocycline inhibits proNGF expression after SCI. A , B , NGF mRNA ( A ) and protein expression ( B ) were increased after SCI. B , C , Anti-mature-NGF antibody detected both mature (14 kDa) and proNGF (26 kDa) ( B ), and anti-proNGF antibody detected the high-molecular-weight band of 26 kDa ( C ). Note that the extent of induction of proNGF was greater than that observed with mature NGF ( B ). D , Minocycline treatment decreased the level of proNGF expression compared with that observed in vehicle-treated control at 5 d after injury. E , Quantitative analysis of Western blots shows that minocycline significantly inhibited proNGF expression when compared with that in vehicle control at 5 d after injury. Values are mean ± SD of three separate experiments. * p
Techniques Used: Expressing, Molecular Weight, Western Blot
Figure Legend Snippet: A , Microglia-derived proNGF induces apoptosis of oligodendrocytes in culture. Immunocytochemical analysis shows that MBP-positive oligodendrocytes expressed p75 NTR (arrows). Scale bar, 20 μm. Differentiated oligodendrocytes were treated with LPS-treated BV2 cell culture medium. After 24 h, cells were processed for TUNEL and MBP staining. B , Representative photographs show that LPS-treated BV2 cell culture medium or recombinant NGF (as a positive control) induced the apoptotic cell death of oligodendrocytes as revealed by the presence of both TUNEL- and MBP-positive cells (arrows). Note that control shows no TUNEL/MBP-positive cells. Scale bar, 20 μm. C , Quantitative analyses of TUNEL-positive oligodendrocytes show that LPS-induced oligodendrocyte cell death was significantly inhibited by minocycline or SB203580 treatment. Also, oligodendrocyte cell death was significantly attenuated when LPS-treated BV2 cell culture medium subjected to immunoprecipitation using a neutralizing anti-NGF polyclonal antibody or when oligodendrocytes were treated with anti- p75 NTR antibody before treatment with LPS-treated BV2 cell culture medium ( C ). Values are mean ± SD of three separate experiments. * p
Techniques Used: Derivative Assay, Cell Culture, TUNEL Assay, Staining, Recombinant, Positive Control, Immunoprecipitation