Article Title: NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer
Figure Lengend Snippet: In vitro growth inhibition of NRXN1-targeted ADC. ( A – F ) In vitro growth inhibition of anti-NRXN1 monoclonal antibody only, secondary ADC only, isotype control antibody (IgG) followed by secondary ADC, and anti-NRXN1 monoclonal antibody followed by secondary ADC on incubation with (A) SHP77, (B) SHP77 KO, (C) HEK293, (D) HEK293-NRXN1, (E) NCI-H526, and (F) PDC. All the assays were performed in triplicate. A two-way ANOVA followed by the Tukey test was performed to assess the difference between IgG with the second ADC group and anti-NRXN1 mAb with the second ADC group. A P value
Article Snippet: Flow cytometry Cells were incubated with 2.5 μL of rabbit anti-NRXN1 polyclonal antibody (ANR-031; Alomone Labs) or an isotype control at 1 × 105 cells/100 μL in PBS with 2% FBS for 30 min in the dark.
Techniques: In Vitro, Inhibition, Incubation