atto488  (Alomone Labs)


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    Alomone Labs atto488
    Atto488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atto488/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atto488 - by Bioz Stars, 2022-12
    93/100 stars

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    Alomone Labs atto488
    Atto488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atto488/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atto488 - by Bioz Stars, 2022-12
    93/100 stars
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    93
    Alomone Labs rabbit polyclonal anti neurexin 1α
    <t>NRXN1</t> expression in normal multiple organs in humans. The relative expressions of NRXN1 in normal multiple organs of human tissue were examined using qRT-PCR with the SYBR green dye assay. The y-axis shows the NRXN1 expression levels relative to normal lung tissue. The y-axis is set to the same scale as that of Figure 1D so that the expression intensities of both surgical specimens and normal organs can be visually compared. Error bars, SD.
    Rabbit Polyclonal Anti Neurexin 1α, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti neurexin 1α/product/Alomone Labs
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
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    NRXN1 expression in normal multiple organs in humans. The relative expressions of NRXN1 in normal multiple organs of human tissue were examined using qRT-PCR with the SYBR green dye assay. The y-axis shows the NRXN1 expression levels relative to normal lung tissue. The y-axis is set to the same scale as that of Figure 1D so that the expression intensities of both surgical specimens and normal organs can be visually compared. Error bars, SD.

    Journal: Oncotarget

    Article Title: NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer

    doi: 10.18632/oncotarget.27718

    Figure Lengend Snippet: NRXN1 expression in normal multiple organs in humans. The relative expressions of NRXN1 in normal multiple organs of human tissue were examined using qRT-PCR with the SYBR green dye assay. The y-axis shows the NRXN1 expression levels relative to normal lung tissue. The y-axis is set to the same scale as that of Figure 1D so that the expression intensities of both surgical specimens and normal organs can be visually compared. Error bars, SD.

    Article Snippet: Flow cytometry Cells were incubated with 2.5 μL of rabbit anti-NRXN1 polyclonal antibody (ANR-031; Alomone Labs) or an isotype control at 1 × 105 cells/100 μL in PBS with 2% FBS for 30 min in the dark.

    Techniques: Expressing, Quantitative RT-PCR, SYBR Green Assay

    NRXN1 expression in cell lines and surgical specimens of lung tissue including non-SCLC and normal tissues. ( A ) The relative expressions in cell lines were examined using qRT-PCR with the SYBR green dye assay; data for NRXN1 is shown. The log 2 -scale relative gene expression is indicated on the y -axis. Error bars, SD. ( B ) Flow cytometry of NRXN1 on cell lines. Cell surface NRXN1 was assessed using FITC-conjugated anti-rabbit polyclonal antibody following rabbit anti-NRXN1 (Black trace) or IgG isotype control (gray-filled) antibodies. FCM, flow cytometry. ( C ) Percentage of NRXN1-positive cells examined for each cell line using flow cytometry. Cells were stained with rabbit anti-NRXN1 polyclonal antibody followed by FITC conjugated goat anti-rabbit IgG. A one-way analysis of variance (ANOVA) followed by the Tukey test was performed. ( * P

    Journal: Oncotarget

    Article Title: NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer

    doi: 10.18632/oncotarget.27718

    Figure Lengend Snippet: NRXN1 expression in cell lines and surgical specimens of lung tissue including non-SCLC and normal tissues. ( A ) The relative expressions in cell lines were examined using qRT-PCR with the SYBR green dye assay; data for NRXN1 is shown. The log 2 -scale relative gene expression is indicated on the y -axis. Error bars, SD. ( B ) Flow cytometry of NRXN1 on cell lines. Cell surface NRXN1 was assessed using FITC-conjugated anti-rabbit polyclonal antibody following rabbit anti-NRXN1 (Black trace) or IgG isotype control (gray-filled) antibodies. FCM, flow cytometry. ( C ) Percentage of NRXN1-positive cells examined for each cell line using flow cytometry. Cells were stained with rabbit anti-NRXN1 polyclonal antibody followed by FITC conjugated goat anti-rabbit IgG. A one-way analysis of variance (ANOVA) followed by the Tukey test was performed. ( * P

    Article Snippet: Flow cytometry Cells were incubated with 2.5 μL of rabbit anti-NRXN1 polyclonal antibody (ANR-031; Alomone Labs) or an isotype control at 1 × 105 cells/100 μL in PBS with 2% FBS for 30 min in the dark.

    Techniques: Expressing, Quantitative RT-PCR, SYBR Green Assay, Flow Cytometry, Staining

    In vitro growth inhibition of NRXN1-targeted ADC. ( A – F ) In vitro growth inhibition of anti-NRXN1 monoclonal antibody only, secondary ADC only, isotype control antibody (IgG) followed by secondary ADC, and anti-NRXN1 monoclonal antibody followed by secondary ADC on incubation with (A) SHP77, (B) SHP77 KO, (C) HEK293, (D) HEK293-NRXN1, (E) NCI-H526, and (F) PDC. All the assays were performed in triplicate. A two-way ANOVA followed by the Tukey test was performed to assess the difference between IgG with the second ADC group and anti-NRXN1 mAb with the second ADC group. A P value

    Journal: Oncotarget

    Article Title: NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer

    doi: 10.18632/oncotarget.27718

    Figure Lengend Snippet: In vitro growth inhibition of NRXN1-targeted ADC. ( A – F ) In vitro growth inhibition of anti-NRXN1 monoclonal antibody only, secondary ADC only, isotype control antibody (IgG) followed by secondary ADC, and anti-NRXN1 monoclonal antibody followed by secondary ADC on incubation with (A) SHP77, (B) SHP77 KO, (C) HEK293, (D) HEK293-NRXN1, (E) NCI-H526, and (F) PDC. All the assays were performed in triplicate. A two-way ANOVA followed by the Tukey test was performed to assess the difference between IgG with the second ADC group and anti-NRXN1 mAb with the second ADC group. A P value

    Article Snippet: Flow cytometry Cells were incubated with 2.5 μL of rabbit anti-NRXN1 polyclonal antibody (ANR-031; Alomone Labs) or an isotype control at 1 × 105 cells/100 μL in PBS with 2% FBS for 30 min in the dark.

    Techniques: In Vitro, Inhibition, Incubation

    Apoptosis assay of NRXN1-targeted ADC at IC 50 dose calculated by growth inhibition curves. Late apoptotic cells were quantified by Cy7-conjugated annexin-V and PI using flow cytometry. Results were analyzed using a one-way ANOVA followed by the Dunnett multiple comparisons test ( * P

    Journal: Oncotarget

    Article Title: NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer

    doi: 10.18632/oncotarget.27718

    Figure Lengend Snippet: Apoptosis assay of NRXN1-targeted ADC at IC 50 dose calculated by growth inhibition curves. Late apoptotic cells were quantified by Cy7-conjugated annexin-V and PI using flow cytometry. Results were analyzed using a one-way ANOVA followed by the Dunnett multiple comparisons test ( * P

    Article Snippet: Flow cytometry Cells were incubated with 2.5 μL of rabbit anti-NRXN1 polyclonal antibody (ANR-031; Alomone Labs) or an isotype control at 1 × 105 cells/100 μL in PBS with 2% FBS for 30 min in the dark.

    Techniques: Apoptosis Assay, Inhibition, Flow Cytometry