rabbit anti npy1r  (Alomone Labs)


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    Alomone Labs rabbit anti npy1r
    Expression of <t>NPY1R</t> in the porcine ventricular myocardium and the effects of infusion of BIBO 3304 on hemodynamic parameters and ARIs. ( A ) NPY1R expression was confirmed in the porcine heart. High expression of NPY1R (green) is evident in the vascular smooth muscle, with moderate expression in intracardiac ganglia and myocardium. NPY-immunoreactive nerve fibers (red) directly appose the NPY1R-immunoreactive vessels and myocardium. ( B ) The presence of NPY1R is confirmed by Western blot analysis of ventricular whole cell lysate and shows no regional differences in expression. BIBO 3304 had no significant effect on resting ( C ) HR or ( D ) dP/dt max , while a modest reduction in ( E ) LVSP (101.6 ± 4.1 to 94.7 ± 5.7 mmHg) was observed. ( F ) Representative polar maps comparing the effects of BIBO 3304 on raw ARIs. ( G ) Global corrected ventricular ARIs were significantly prolonged following the administration of BIBO 3304. Scale bars: 10 μm. A and B : n = 3 animals for all comparisons; comparisons were performed using 1-way ANOVA with post hoc analysis. C – F : n = 7 animals for all comparisons; comparisons were performed using 2-sided paired Student’s t test. “Before BIBO” refers to data recorded just before infusion of BIBO 3304 but after infusion of 1.0 mg/kg propranolol for protocol 3, while “After BIBO” represents data taken after 20 minutes of BIBO 3304 infusion.
    Rabbit Anti Npy1r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti npy1r/product/Alomone Labs
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit anti npy1r - by Bioz Stars, 2022-09
    92/100 stars

    Images

    1) Product Images from "Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y"

    Article Title: Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y

    Journal: JCI Insight

    doi: 10.1172/jci.insight.135519

    Expression of NPY1R in the porcine ventricular myocardium and the effects of infusion of BIBO 3304 on hemodynamic parameters and ARIs. ( A ) NPY1R expression was confirmed in the porcine heart. High expression of NPY1R (green) is evident in the vascular smooth muscle, with moderate expression in intracardiac ganglia and myocardium. NPY-immunoreactive nerve fibers (red) directly appose the NPY1R-immunoreactive vessels and myocardium. ( B ) The presence of NPY1R is confirmed by Western blot analysis of ventricular whole cell lysate and shows no regional differences in expression. BIBO 3304 had no significant effect on resting ( C ) HR or ( D ) dP/dt max , while a modest reduction in ( E ) LVSP (101.6 ± 4.1 to 94.7 ± 5.7 mmHg) was observed. ( F ) Representative polar maps comparing the effects of BIBO 3304 on raw ARIs. ( G ) Global corrected ventricular ARIs were significantly prolonged following the administration of BIBO 3304. Scale bars: 10 μm. A and B : n = 3 animals for all comparisons; comparisons were performed using 1-way ANOVA with post hoc analysis. C – F : n = 7 animals for all comparisons; comparisons were performed using 2-sided paired Student’s t test. “Before BIBO” refers to data recorded just before infusion of BIBO 3304 but after infusion of 1.0 mg/kg propranolol for protocol 3, while “After BIBO” represents data taken after 20 minutes of BIBO 3304 infusion.
    Figure Legend Snippet: Expression of NPY1R in the porcine ventricular myocardium and the effects of infusion of BIBO 3304 on hemodynamic parameters and ARIs. ( A ) NPY1R expression was confirmed in the porcine heart. High expression of NPY1R (green) is evident in the vascular smooth muscle, with moderate expression in intracardiac ganglia and myocardium. NPY-immunoreactive nerve fibers (red) directly appose the NPY1R-immunoreactive vessels and myocardium. ( B ) The presence of NPY1R is confirmed by Western blot analysis of ventricular whole cell lysate and shows no regional differences in expression. BIBO 3304 had no significant effect on resting ( C ) HR or ( D ) dP/dt max , while a modest reduction in ( E ) LVSP (101.6 ± 4.1 to 94.7 ± 5.7 mmHg) was observed. ( F ) Representative polar maps comparing the effects of BIBO 3304 on raw ARIs. ( G ) Global corrected ventricular ARIs were significantly prolonged following the administration of BIBO 3304. Scale bars: 10 μm. A and B : n = 3 animals for all comparisons; comparisons were performed using 1-way ANOVA with post hoc analysis. C – F : n = 7 animals for all comparisons; comparisons were performed using 2-sided paired Student’s t test. “Before BIBO” refers to data recorded just before infusion of BIBO 3304 but after infusion of 1.0 mg/kg propranolol for protocol 3, while “After BIBO” represents data taken after 20 minutes of BIBO 3304 infusion.

    Techniques Used: Expressing, Western Blot

    Effects of BSS on hemodynamic and electrophysiological parameters after 1.0 mg/kg propranolol i.v. and BIBO 3304 as compared with propranolol alone. BIBO 3304 completely blocked the effects of BSS on ( A ) HR, while residual effects on ( B ) LVSP and ( C ) dP/dt max continued to be observed. BIBO 3304 augmented the changes in dP/dt max . ( D ) Representative polar maps comparing effects of BSS on raw ventricular ARIs in the setting of high-dose propranolol, with and without BIBO 3304. ( E ) Global corrected ventricular ARIs significantly shortened despite administration of high-dose propranolol, but not after administration of BIBO 3304. Additional treatment with BIBO 3304 significantly reduced BBS-induced shortening of ARIc. (+) prop, 1.0 mg/kg propranolol, (–) BIBO, no NPY1R blockade, (+) BIBO, 0.2 mg/kg + 0.4 mg/kg/h BIBO 3304. n = 10 animals for all comparisons, analyses were performed using the 2-sided paired Student’s t test.
    Figure Legend Snippet: Effects of BSS on hemodynamic and electrophysiological parameters after 1.0 mg/kg propranolol i.v. and BIBO 3304 as compared with propranolol alone. BIBO 3304 completely blocked the effects of BSS on ( A ) HR, while residual effects on ( B ) LVSP and ( C ) dP/dt max continued to be observed. BIBO 3304 augmented the changes in dP/dt max . ( D ) Representative polar maps comparing effects of BSS on raw ventricular ARIs in the setting of high-dose propranolol, with and without BIBO 3304. ( E ) Global corrected ventricular ARIs significantly shortened despite administration of high-dose propranolol, but not after administration of BIBO 3304. Additional treatment with BIBO 3304 significantly reduced BBS-induced shortening of ARIc. (+) prop, 1.0 mg/kg propranolol, (–) BIBO, no NPY1R blockade, (+) BIBO, 0.2 mg/kg + 0.4 mg/kg/h BIBO 3304. n = 10 animals for all comparisons, analyses were performed using the 2-sided paired Student’s t test.

    Techniques Used:

    2) Product Images from "Altered expression of neuropeptide Y receptors caused by focal cortical dysplasia in human intractable epilepsy"

    Article Title: Altered expression of neuropeptide Y receptors caused by focal cortical dysplasia in human intractable epilepsy

    Journal: Oncotarget

    doi: 10.18632/oncotarget.7855

    The expression and distribution of Y1R in different cortical lobes of FCD patients A. - C. Representative images show Y1R (green) and DAPI (blue) in temporal lobe (A), frontal lobe (B) and parietal lobe (C) with upper panels showing control and lower panels showing patients. The last images are the enlarged views from areas indicated by squares. C, control; P, patient. D. The quantification of Y1R fluorescent intensities of A-C. E. Representative Western blots of Y1R proteins in the temporal, frontal and parietal lobe. F. Bar graphs show quantitative data for Y1R signals that are normalized to GAPDH signal. G. Quantitative PCR array analysis of the expression of Y1R in the temporal, frontal and parietal lobe. Data are expressed by means ± SEM. * P
    Figure Legend Snippet: The expression and distribution of Y1R in different cortical lobes of FCD patients A. - C. Representative images show Y1R (green) and DAPI (blue) in temporal lobe (A), frontal lobe (B) and parietal lobe (C) with upper panels showing control and lower panels showing patients. The last images are the enlarged views from areas indicated by squares. C, control; P, patient. D. The quantification of Y1R fluorescent intensities of A-C. E. Representative Western blots of Y1R proteins in the temporal, frontal and parietal lobe. F. Bar graphs show quantitative data for Y1R signals that are normalized to GAPDH signal. G. Quantitative PCR array analysis of the expression of Y1R in the temporal, frontal and parietal lobe. Data are expressed by means ± SEM. * P

    Techniques Used: Expressing, Western Blot, Real-time Polymerase Chain Reaction

    3) Product Images from "Altered expression of neuropeptide Y receptors caused by focal cortical dysplasia in human intractable epilepsy"

    Article Title: Altered expression of neuropeptide Y receptors caused by focal cortical dysplasia in human intractable epilepsy

    Journal: Oncotarget

    doi: 10.18632/oncotarget.7855

    The expression and distribution of Y1R in different cortical lobes of FCD patients A. - C. Representative images show Y1R (green) and DAPI (blue) in temporal lobe (A), frontal lobe (B) and parietal lobe (C) with upper panels showing control and lower panels showing patients. The last images are the enlarged views from areas indicated by squares. C, control; P, patient. D. The quantification of Y1R fluorescent intensities of A-C. E. Representative Western blots of Y1R proteins in the temporal, frontal and parietal lobe. F. Bar graphs show quantitative data for Y1R signals that are normalized to GAPDH signal. G. Quantitative PCR array analysis of the expression of Y1R in the temporal, frontal and parietal lobe. Data are expressed by means ± SEM. * P
    Figure Legend Snippet: The expression and distribution of Y1R in different cortical lobes of FCD patients A. - C. Representative images show Y1R (green) and DAPI (blue) in temporal lobe (A), frontal lobe (B) and parietal lobe (C) with upper panels showing control and lower panels showing patients. The last images are the enlarged views from areas indicated by squares. C, control; P, patient. D. The quantification of Y1R fluorescent intensities of A-C. E. Representative Western blots of Y1R proteins in the temporal, frontal and parietal lobe. F. Bar graphs show quantitative data for Y1R signals that are normalized to GAPDH signal. G. Quantitative PCR array analysis of the expression of Y1R in the temporal, frontal and parietal lobe. Data are expressed by means ± SEM. * P

    Techniques Used: Expressing, Western Blot, Real-time Polymerase Chain Reaction

    4) Product Images from "Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y"

    Article Title: Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y

    Journal: JCI Insight

    doi: 10.1172/jci.insight.135519

    Expression of NPY1R in the porcine ventricular myocardium and the effects of infusion of BIBO 3304 on hemodynamic parameters and ARIs. ( A ) NPY1R expression was confirmed in the porcine heart. High expression of NPY1R (green) is evident in the vascular smooth muscle, with moderate expression in intracardiac ganglia and myocardium. NPY-immunoreactive nerve fibers (red) directly appose the NPY1R-immunoreactive vessels and myocardium. ( B ) The presence of NPY1R is confirmed by Western blot analysis of ventricular whole cell lysate and shows no regional differences in expression. BIBO 3304 had no significant effect on resting ( C ) HR or ( D ) dP/dt max , while a modest reduction in ( E ) LVSP (101.6 ± 4.1 to 94.7 ± 5.7 mmHg) was observed. ( F ) Representative polar maps comparing the effects of BIBO 3304 on raw ARIs. ( G ) Global corrected ventricular ARIs were significantly prolonged following the administration of BIBO 3304. Scale bars: 10 μm. A and B : n = 3 animals for all comparisons; comparisons were performed using 1-way ANOVA with post hoc analysis. C – F : n = 7 animals for all comparisons; comparisons were performed using 2-sided paired Student’s t test. “Before BIBO” refers to data recorded just before infusion of BIBO 3304 but after infusion of 1.0 mg/kg propranolol for protocol 3, while “After BIBO” represents data taken after 20 minutes of BIBO 3304 infusion.
    Figure Legend Snippet: Expression of NPY1R in the porcine ventricular myocardium and the effects of infusion of BIBO 3304 on hemodynamic parameters and ARIs. ( A ) NPY1R expression was confirmed in the porcine heart. High expression of NPY1R (green) is evident in the vascular smooth muscle, with moderate expression in intracardiac ganglia and myocardium. NPY-immunoreactive nerve fibers (red) directly appose the NPY1R-immunoreactive vessels and myocardium. ( B ) The presence of NPY1R is confirmed by Western blot analysis of ventricular whole cell lysate and shows no regional differences in expression. BIBO 3304 had no significant effect on resting ( C ) HR or ( D ) dP/dt max , while a modest reduction in ( E ) LVSP (101.6 ± 4.1 to 94.7 ± 5.7 mmHg) was observed. ( F ) Representative polar maps comparing the effects of BIBO 3304 on raw ARIs. ( G ) Global corrected ventricular ARIs were significantly prolonged following the administration of BIBO 3304. Scale bars: 10 μm. A and B : n = 3 animals for all comparisons; comparisons were performed using 1-way ANOVA with post hoc analysis. C – F : n = 7 animals for all comparisons; comparisons were performed using 2-sided paired Student’s t test. “Before BIBO” refers to data recorded just before infusion of BIBO 3304 but after infusion of 1.0 mg/kg propranolol for protocol 3, while “After BIBO” represents data taken after 20 minutes of BIBO 3304 infusion.

    Techniques Used: Expressing, Western Blot

    Effects of BSS on hemodynamic and electrophysiological parameters after 1.0 mg/kg propranolol i.v. and BIBO 3304 as compared with propranolol alone. BIBO 3304 completely blocked the effects of BSS on ( A ) HR, while residual effects on ( B ) LVSP and ( C ) dP/dt max continued to be observed. BIBO 3304 augmented the changes in dP/dt max . ( D ) Representative polar maps comparing effects of BSS on raw ventricular ARIs in the setting of high-dose propranolol, with and without BIBO 3304. ( E ) Global corrected ventricular ARIs significantly shortened despite administration of high-dose propranolol, but not after administration of BIBO 3304. Additional treatment with BIBO 3304 significantly reduced BBS-induced shortening of ARIc. (+) prop, 1.0 mg/kg propranolol, (–) BIBO, no NPY1R blockade, (+) BIBO, 0.2 mg/kg + 0.4 mg/kg/h BIBO 3304. n = 10 animals for all comparisons, analyses were performed using the 2-sided paired Student’s t test.
    Figure Legend Snippet: Effects of BSS on hemodynamic and electrophysiological parameters after 1.0 mg/kg propranolol i.v. and BIBO 3304 as compared with propranolol alone. BIBO 3304 completely blocked the effects of BSS on ( A ) HR, while residual effects on ( B ) LVSP and ( C ) dP/dt max continued to be observed. BIBO 3304 augmented the changes in dP/dt max . ( D ) Representative polar maps comparing effects of BSS on raw ventricular ARIs in the setting of high-dose propranolol, with and without BIBO 3304. ( E ) Global corrected ventricular ARIs significantly shortened despite administration of high-dose propranolol, but not after administration of BIBO 3304. Additional treatment with BIBO 3304 significantly reduced BBS-induced shortening of ARIc. (+) prop, 1.0 mg/kg propranolol, (–) BIBO, no NPY1R blockade, (+) BIBO, 0.2 mg/kg + 0.4 mg/kg/h BIBO 3304. n = 10 animals for all comparisons, analyses were performed using the 2-sided paired Student’s t test.

    Techniques Used:

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    Alomone Labs rabbit anti npy1r
    Expression of <t>NPY1R</t> in the porcine ventricular myocardium and the effects of infusion of BIBO 3304 on hemodynamic parameters and ARIs. ( A ) NPY1R expression was confirmed in the porcine heart. High expression of NPY1R (green) is evident in the vascular smooth muscle, with moderate expression in intracardiac ganglia and myocardium. NPY-immunoreactive nerve fibers (red) directly appose the NPY1R-immunoreactive vessels and myocardium. ( B ) The presence of NPY1R is confirmed by Western blot analysis of ventricular whole cell lysate and shows no regional differences in expression. BIBO 3304 had no significant effect on resting ( C ) HR or ( D ) dP/dt max , while a modest reduction in ( E ) LVSP (101.6 ± 4.1 to 94.7 ± 5.7 mmHg) was observed. ( F ) Representative polar maps comparing the effects of BIBO 3304 on raw ARIs. ( G ) Global corrected ventricular ARIs were significantly prolonged following the administration of BIBO 3304. Scale bars: 10 μm. A and B : n = 3 animals for all comparisons; comparisons were performed using 1-way ANOVA with post hoc analysis. C – F : n = 7 animals for all comparisons; comparisons were performed using 2-sided paired Student’s t test. “Before BIBO” refers to data recorded just before infusion of BIBO 3304 but after infusion of 1.0 mg/kg propranolol for protocol 3, while “After BIBO” represents data taken after 20 minutes of BIBO 3304 infusion.
    Rabbit Anti Npy1r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti npy1r/product/Alomone Labs
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit anti npy1r - by Bioz Stars, 2022-09
    92/100 stars
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    Expression of NPY1R in the porcine ventricular myocardium and the effects of infusion of BIBO 3304 on hemodynamic parameters and ARIs. ( A ) NPY1R expression was confirmed in the porcine heart. High expression of NPY1R (green) is evident in the vascular smooth muscle, with moderate expression in intracardiac ganglia and myocardium. NPY-immunoreactive nerve fibers (red) directly appose the NPY1R-immunoreactive vessels and myocardium. ( B ) The presence of NPY1R is confirmed by Western blot analysis of ventricular whole cell lysate and shows no regional differences in expression. BIBO 3304 had no significant effect on resting ( C ) HR or ( D ) dP/dt max , while a modest reduction in ( E ) LVSP (101.6 ± 4.1 to 94.7 ± 5.7 mmHg) was observed. ( F ) Representative polar maps comparing the effects of BIBO 3304 on raw ARIs. ( G ) Global corrected ventricular ARIs were significantly prolonged following the administration of BIBO 3304. Scale bars: 10 μm. A and B : n = 3 animals for all comparisons; comparisons were performed using 1-way ANOVA with post hoc analysis. C – F : n = 7 animals for all comparisons; comparisons were performed using 2-sided paired Student’s t test. “Before BIBO” refers to data recorded just before infusion of BIBO 3304 but after infusion of 1.0 mg/kg propranolol for protocol 3, while “After BIBO” represents data taken after 20 minutes of BIBO 3304 infusion.

    Journal: JCI Insight

    Article Title: Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y

    doi: 10.1172/jci.insight.135519

    Figure Lengend Snippet: Expression of NPY1R in the porcine ventricular myocardium and the effects of infusion of BIBO 3304 on hemodynamic parameters and ARIs. ( A ) NPY1R expression was confirmed in the porcine heart. High expression of NPY1R (green) is evident in the vascular smooth muscle, with moderate expression in intracardiac ganglia and myocardium. NPY-immunoreactive nerve fibers (red) directly appose the NPY1R-immunoreactive vessels and myocardium. ( B ) The presence of NPY1R is confirmed by Western blot analysis of ventricular whole cell lysate and shows no regional differences in expression. BIBO 3304 had no significant effect on resting ( C ) HR or ( D ) dP/dt max , while a modest reduction in ( E ) LVSP (101.6 ± 4.1 to 94.7 ± 5.7 mmHg) was observed. ( F ) Representative polar maps comparing the effects of BIBO 3304 on raw ARIs. ( G ) Global corrected ventricular ARIs were significantly prolonged following the administration of BIBO 3304. Scale bars: 10 μm. A and B : n = 3 animals for all comparisons; comparisons were performed using 1-way ANOVA with post hoc analysis. C – F : n = 7 animals for all comparisons; comparisons were performed using 2-sided paired Student’s t test. “Before BIBO” refers to data recorded just before infusion of BIBO 3304 but after infusion of 1.0 mg/kg propranolol for protocol 3, while “After BIBO” represents data taken after 20 minutes of BIBO 3304 infusion.

    Article Snippet: Slides were then blocked for 1 hour in 3% BSA-TBS/0.2% Triton X-100 with 5% donkey serum and incubated overnight at 4°C with rabbit anti-NPY1R (1:200; Alomone Labs, ANR-021) and mouse anti-NPY (1:500; Abcam, ab112473), followed by 2-hour incubation at room temperature with Alexa Fluor 488–donkey anti-rabbit IgG (1:200; Invitrogen, A-21206) and Alexa Fluor 555–donkey anti-mouse IgG (1:200; Invitrogen, A-31570).

    Techniques: Expressing, Western Blot

    Effects of BSS on hemodynamic and electrophysiological parameters after 1.0 mg/kg propranolol i.v. and BIBO 3304 as compared with propranolol alone. BIBO 3304 completely blocked the effects of BSS on ( A ) HR, while residual effects on ( B ) LVSP and ( C ) dP/dt max continued to be observed. BIBO 3304 augmented the changes in dP/dt max . ( D ) Representative polar maps comparing effects of BSS on raw ventricular ARIs in the setting of high-dose propranolol, with and without BIBO 3304. ( E ) Global corrected ventricular ARIs significantly shortened despite administration of high-dose propranolol, but not after administration of BIBO 3304. Additional treatment with BIBO 3304 significantly reduced BBS-induced shortening of ARIc. (+) prop, 1.0 mg/kg propranolol, (–) BIBO, no NPY1R blockade, (+) BIBO, 0.2 mg/kg + 0.4 mg/kg/h BIBO 3304. n = 10 animals for all comparisons, analyses were performed using the 2-sided paired Student’s t test.

    Journal: JCI Insight

    Article Title: Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y

    doi: 10.1172/jci.insight.135519

    Figure Lengend Snippet: Effects of BSS on hemodynamic and electrophysiological parameters after 1.0 mg/kg propranolol i.v. and BIBO 3304 as compared with propranolol alone. BIBO 3304 completely blocked the effects of BSS on ( A ) HR, while residual effects on ( B ) LVSP and ( C ) dP/dt max continued to be observed. BIBO 3304 augmented the changes in dP/dt max . ( D ) Representative polar maps comparing effects of BSS on raw ventricular ARIs in the setting of high-dose propranolol, with and without BIBO 3304. ( E ) Global corrected ventricular ARIs significantly shortened despite administration of high-dose propranolol, but not after administration of BIBO 3304. Additional treatment with BIBO 3304 significantly reduced BBS-induced shortening of ARIc. (+) prop, 1.0 mg/kg propranolol, (–) BIBO, no NPY1R blockade, (+) BIBO, 0.2 mg/kg + 0.4 mg/kg/h BIBO 3304. n = 10 animals for all comparisons, analyses were performed using the 2-sided paired Student’s t test.

    Article Snippet: Slides were then blocked for 1 hour in 3% BSA-TBS/0.2% Triton X-100 with 5% donkey serum and incubated overnight at 4°C with rabbit anti-NPY1R (1:200; Alomone Labs, ANR-021) and mouse anti-NPY (1:500; Abcam, ab112473), followed by 2-hour incubation at room temperature with Alexa Fluor 488–donkey anti-rabbit IgG (1:200; Invitrogen, A-21206) and Alexa Fluor 555–donkey anti-mouse IgG (1:200; Invitrogen, A-31570).

    Techniques:

    The expression and distribution of Y1R in different cortical lobes of FCD patients A. - C. Representative images show Y1R (green) and DAPI (blue) in temporal lobe (A), frontal lobe (B) and parietal lobe (C) with upper panels showing control and lower panels showing patients. The last images are the enlarged views from areas indicated by squares. C, control; P, patient. D. The quantification of Y1R fluorescent intensities of A-C. E. Representative Western blots of Y1R proteins in the temporal, frontal and parietal lobe. F. Bar graphs show quantitative data for Y1R signals that are normalized to GAPDH signal. G. Quantitative PCR array analysis of the expression of Y1R in the temporal, frontal and parietal lobe. Data are expressed by means ± SEM. * P

    Journal: Oncotarget

    Article Title: Altered expression of neuropeptide Y receptors caused by focal cortical dysplasia in human intractable epilepsy

    doi: 10.18632/oncotarget.7855

    Figure Lengend Snippet: The expression and distribution of Y1R in different cortical lobes of FCD patients A. - C. Representative images show Y1R (green) and DAPI (blue) in temporal lobe (A), frontal lobe (B) and parietal lobe (C) with upper panels showing control and lower panels showing patients. The last images are the enlarged views from areas indicated by squares. C, control; P, patient. D. The quantification of Y1R fluorescent intensities of A-C. E. Representative Western blots of Y1R proteins in the temporal, frontal and parietal lobe. F. Bar graphs show quantitative data for Y1R signals that are normalized to GAPDH signal. G. Quantitative PCR array analysis of the expression of Y1R in the temporal, frontal and parietal lobe. Data are expressed by means ± SEM. * P

    Article Snippet: Antibodies against Y1R (1:200, Alomone Labs), Y2R (1:200, Alomone Labs), Y5R (1:200, Alomone Labs), NPY (1:200, Sigma), and GAPDH (1:5000, Sigma) were used in this study.

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction