synaptotagmin  (Alomone Labs)


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    Structured Review

    Alomone Labs synaptotagmin
    WT1-expressing neurons terminate in close proximity to populations of commissurally projecting interneurons. A, A 20-μm-thick section of a spinal cord 46 h after PRV-152 injection into the GS muscle on the left side and stained with antibodies to GFP (green) and WT1 (blue). At this time point, no WT1-expressing neurons on either side of the spinal cord have taken up the tracer. Contralateral region containing WT1-expressing cells (dashed box) is expanded to the right. B, To identify presumptive synapses on genetically defined interneuronal subtypes, we inspected the <t>synaptotagmin+</t> “halo” (indicated by white arrows) surrounding each labeled nuclei for WT1+/synaptotagmin+ terminals. C–F, The 20-μm-thick sections cut from a P0 WT1CreERROSA26tdTomato spinal cord and stained with antibodies to tdTomato (red), the synaptic marker synatotagmin (blue), as well as (green) a nuclear marker of V2a cells (Chx10-, C), V1 cells (En1-, D), V0V cells (Evx1-, E), or DMRT3-expressing dI6 cells (F). WT1-expressing terminals (tdTomato+/synaptotagmin+ processes) were rare or absent nearby Chx10- or En1-expressing cells but were commonly seen in close proximity to Evx1+ and DMRT3+ neurons. C, D, Dashed boxes are expanded to the right. E, F, Arrow in the low-magnification image indicates the specific WT1 cell of interest in the panel to the right. In magnified images, arrows indicate presumptive WT1+ axon terminals. Double labeling of these processes is confirmed in orthogonal views to the right of E, F. Scale bars: Low-magnification images, 100 μm; High-magnification images: A, C, D, 20 μm; B, E, F, 5 μm.
    Synaptotagmin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptotagmin/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    synaptotagmin - by Bioz Stars, 2023-01
    92/100 stars

    Images

    1) Product Images from "WT1 -Expressing Interneurons Regulate Left–Right Alternation during Mammalian Locomotor Activity"

    Article Title: WT1 -Expressing Interneurons Regulate Left–Right Alternation during Mammalian Locomotor Activity

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.0328-18.2018

    WT1-expressing neurons terminate in close proximity to populations of commissurally projecting interneurons. A, A 20-μm-thick section of a spinal cord 46 h after PRV-152 injection into the GS muscle on the left side and stained with antibodies to GFP (green) and WT1 (blue). At this time point, no WT1-expressing neurons on either side of the spinal cord have taken up the tracer. Contralateral region containing WT1-expressing cells (dashed box) is expanded to the right. B, To identify presumptive synapses on genetically defined interneuronal subtypes, we inspected the synaptotagmin+ “halo” (indicated by white arrows) surrounding each labeled nuclei for WT1+/synaptotagmin+ terminals. C–F, The 20-μm-thick sections cut from a P0 WT1CreERROSA26tdTomato spinal cord and stained with antibodies to tdTomato (red), the synaptic marker synatotagmin (blue), as well as (green) a nuclear marker of V2a cells (Chx10-, C), V1 cells (En1-, D), V0V cells (Evx1-, E), or DMRT3-expressing dI6 cells (F). WT1-expressing terminals (tdTomato+/synaptotagmin+ processes) were rare or absent nearby Chx10- or En1-expressing cells but were commonly seen in close proximity to Evx1+ and DMRT3+ neurons. C, D, Dashed boxes are expanded to the right. E, F, Arrow in the low-magnification image indicates the specific WT1 cell of interest in the panel to the right. In magnified images, arrows indicate presumptive WT1+ axon terminals. Double labeling of these processes is confirmed in orthogonal views to the right of E, F. Scale bars: Low-magnification images, 100 μm; High-magnification images: A, C, D, 20 μm; B, E, F, 5 μm.
    Figure Legend Snippet: WT1-expressing neurons terminate in close proximity to populations of commissurally projecting interneurons. A, A 20-μm-thick section of a spinal cord 46 h after PRV-152 injection into the GS muscle on the left side and stained with antibodies to GFP (green) and WT1 (blue). At this time point, no WT1-expressing neurons on either side of the spinal cord have taken up the tracer. Contralateral region containing WT1-expressing cells (dashed box) is expanded to the right. B, To identify presumptive synapses on genetically defined interneuronal subtypes, we inspected the synaptotagmin+ “halo” (indicated by white arrows) surrounding each labeled nuclei for WT1+/synaptotagmin+ terminals. C–F, The 20-μm-thick sections cut from a P0 WT1CreERROSA26tdTomato spinal cord and stained with antibodies to tdTomato (red), the synaptic marker synatotagmin (blue), as well as (green) a nuclear marker of V2a cells (Chx10-, C), V1 cells (En1-, D), V0V cells (Evx1-, E), or DMRT3-expressing dI6 cells (F). WT1-expressing terminals (tdTomato+/synaptotagmin+ processes) were rare or absent nearby Chx10- or En1-expressing cells but were commonly seen in close proximity to Evx1+ and DMRT3+ neurons. C, D, Dashed boxes are expanded to the right. E, F, Arrow in the low-magnification image indicates the specific WT1 cell of interest in the panel to the right. In magnified images, arrows indicate presumptive WT1+ axon terminals. Double labeling of these processes is confirmed in orthogonal views to the right of E, F. Scale bars: Low-magnification images, 100 μm; High-magnification images: A, C, D, 20 μm; B, E, F, 5 μm.

    Techniques Used: Expressing, Injection, Staining, Labeling, Marker

    synaptotagmin  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
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  • 92

    Structured Review

    Alomone Labs synaptotagmin
    WT1-expressing neurons terminate in close proximity to populations of commissurally projecting interneurons. A, A 20-μm-thick section of a spinal cord 46 h after PRV-152 injection into the GS muscle on the left side and stained with antibodies to GFP (green) and WT1 (blue). At this time point, no WT1-expressing neurons on either side of the spinal cord have taken up the tracer. Contralateral region containing WT1-expressing cells (dashed box) is expanded to the right. B, To identify presumptive synapses on genetically defined interneuronal subtypes, we inspected the <t>synaptotagmin+</t> “halo” (indicated by white arrows) surrounding each labeled nuclei for WT1+/synaptotagmin+ terminals. C–F, The 20-μm-thick sections cut from a P0 WT1CreERROSA26tdTomato spinal cord and stained with antibodies to tdTomato (red), the synaptic marker synatotagmin (blue), as well as (green) a nuclear marker of V2a cells (Chx10-, C), V1 cells (En1-, D), V0V cells (Evx1-, E), or DMRT3-expressing dI6 cells (F). WT1-expressing terminals (tdTomato+/synaptotagmin+ processes) were rare or absent nearby Chx10- or En1-expressing cells but were commonly seen in close proximity to Evx1+ and DMRT3+ neurons. C, D, Dashed boxes are expanded to the right. E, F, Arrow in the low-magnification image indicates the specific WT1 cell of interest in the panel to the right. In magnified images, arrows indicate presumptive WT1+ axon terminals. Double labeling of these processes is confirmed in orthogonal views to the right of E, F. Scale bars: Low-magnification images, 100 μm; High-magnification images: A, C, D, 20 μm; B, E, F, 5 μm.
    Synaptotagmin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptotagmin/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    synaptotagmin - by Bioz Stars, 2023-01
    92/100 stars

    Images

    1) Product Images from "WT1 -Expressing Interneurons Regulate Left–Right Alternation during Mammalian Locomotor Activity"

    Article Title: WT1 -Expressing Interneurons Regulate Left–Right Alternation during Mammalian Locomotor Activity

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.0328-18.2018

    WT1-expressing neurons terminate in close proximity to populations of commissurally projecting interneurons. A, A 20-μm-thick section of a spinal cord 46 h after PRV-152 injection into the GS muscle on the left side and stained with antibodies to GFP (green) and WT1 (blue). At this time point, no WT1-expressing neurons on either side of the spinal cord have taken up the tracer. Contralateral region containing WT1-expressing cells (dashed box) is expanded to the right. B, To identify presumptive synapses on genetically defined interneuronal subtypes, we inspected the synaptotagmin+ “halo” (indicated by white arrows) surrounding each labeled nuclei for WT1+/synaptotagmin+ terminals. C–F, The 20-μm-thick sections cut from a P0 WT1CreERROSA26tdTomato spinal cord and stained with antibodies to tdTomato (red), the synaptic marker synatotagmin (blue), as well as (green) a nuclear marker of V2a cells (Chx10-, C), V1 cells (En1-, D), V0V cells (Evx1-, E), or DMRT3-expressing dI6 cells (F). WT1-expressing terminals (tdTomato+/synaptotagmin+ processes) were rare or absent nearby Chx10- or En1-expressing cells but were commonly seen in close proximity to Evx1+ and DMRT3+ neurons. C, D, Dashed boxes are expanded to the right. E, F, Arrow in the low-magnification image indicates the specific WT1 cell of interest in the panel to the right. In magnified images, arrows indicate presumptive WT1+ axon terminals. Double labeling of these processes is confirmed in orthogonal views to the right of E, F. Scale bars: Low-magnification images, 100 μm; High-magnification images: A, C, D, 20 μm; B, E, F, 5 μm.
    Figure Legend Snippet: WT1-expressing neurons terminate in close proximity to populations of commissurally projecting interneurons. A, A 20-μm-thick section of a spinal cord 46 h after PRV-152 injection into the GS muscle on the left side and stained with antibodies to GFP (green) and WT1 (blue). At this time point, no WT1-expressing neurons on either side of the spinal cord have taken up the tracer. Contralateral region containing WT1-expressing cells (dashed box) is expanded to the right. B, To identify presumptive synapses on genetically defined interneuronal subtypes, we inspected the synaptotagmin+ “halo” (indicated by white arrows) surrounding each labeled nuclei for WT1+/synaptotagmin+ terminals. C–F, The 20-μm-thick sections cut from a P0 WT1CreERROSA26tdTomato spinal cord and stained with antibodies to tdTomato (red), the synaptic marker synatotagmin (blue), as well as (green) a nuclear marker of V2a cells (Chx10-, C), V1 cells (En1-, D), V0V cells (Evx1-, E), or DMRT3-expressing dI6 cells (F). WT1-expressing terminals (tdTomato+/synaptotagmin+ processes) were rare or absent nearby Chx10- or En1-expressing cells but were commonly seen in close proximity to Evx1+ and DMRT3+ neurons. C, D, Dashed boxes are expanded to the right. E, F, Arrow in the low-magnification image indicates the specific WT1 cell of interest in the panel to the right. In magnified images, arrows indicate presumptive WT1+ axon terminals. Double labeling of these processes is confirmed in orthogonal views to the right of E, F. Scale bars: Low-magnification images, 100 μm; High-magnification images: A, C, D, 20 μm; B, E, F, 5 μm.

    Techniques Used: Expressing, Injection, Staining, Labeling, Marker

    synaptotagmin  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Alomone Labs synaptotagmin
    WT1-expressing neurons terminate in close proximity to populations of commissurally projecting interneurons. A, A 20-μm-thick section of a spinal cord 46 h after PRV-152 injection into the GS muscle on the left side and stained with antibodies to GFP (green) and WT1 (blue). At this time point, no WT1-expressing neurons on either side of the spinal cord have taken up the tracer. Contralateral region containing WT1-expressing cells (dashed box) is expanded to the right. B, To identify presumptive synapses on genetically defined interneuronal subtypes, we inspected the <t>synaptotagmin+</t> “halo” (indicated by white arrows) surrounding each labeled nuclei for WT1+/synaptotagmin+ terminals. C–F, The 20-μm-thick sections cut from a P0 WT1CreERROSA26tdTomato spinal cord and stained with antibodies to tdTomato (red), the synaptic marker synatotagmin (blue), as well as (green) a nuclear marker of V2a cells (Chx10-, C), V1 cells (En1-, D), V0V cells (Evx1-, E), or DMRT3-expressing dI6 cells (F). WT1-expressing terminals (tdTomato+/synaptotagmin+ processes) were rare or absent nearby Chx10- or En1-expressing cells but were commonly seen in close proximity to Evx1+ and DMRT3+ neurons. C, D, Dashed boxes are expanded to the right. E, F, Arrow in the low-magnification image indicates the specific WT1 cell of interest in the panel to the right. In magnified images, arrows indicate presumptive WT1+ axon terminals. Double labeling of these processes is confirmed in orthogonal views to the right of E, F. Scale bars: Low-magnification images, 100 μm; High-magnification images: A, C, D, 20 μm; B, E, F, 5 μm.
    Synaptotagmin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptotagmin/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    synaptotagmin - by Bioz Stars, 2023-01
    92/100 stars

    Images

    1) Product Images from "WT1 -Expressing Interneurons Regulate Left–Right Alternation during Mammalian Locomotor Activity"

    Article Title: WT1 -Expressing Interneurons Regulate Left–Right Alternation during Mammalian Locomotor Activity

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.0328-18.2018

    WT1-expressing neurons terminate in close proximity to populations of commissurally projecting interneurons. A, A 20-μm-thick section of a spinal cord 46 h after PRV-152 injection into the GS muscle on the left side and stained with antibodies to GFP (green) and WT1 (blue). At this time point, no WT1-expressing neurons on either side of the spinal cord have taken up the tracer. Contralateral region containing WT1-expressing cells (dashed box) is expanded to the right. B, To identify presumptive synapses on genetically defined interneuronal subtypes, we inspected the synaptotagmin+ “halo” (indicated by white arrows) surrounding each labeled nuclei for WT1+/synaptotagmin+ terminals. C–F, The 20-μm-thick sections cut from a P0 WT1CreERROSA26tdTomato spinal cord and stained with antibodies to tdTomato (red), the synaptic marker synatotagmin (blue), as well as (green) a nuclear marker of V2a cells (Chx10-, C), V1 cells (En1-, D), V0V cells (Evx1-, E), or DMRT3-expressing dI6 cells (F). WT1-expressing terminals (tdTomato+/synaptotagmin+ processes) were rare or absent nearby Chx10- or En1-expressing cells but were commonly seen in close proximity to Evx1+ and DMRT3+ neurons. C, D, Dashed boxes are expanded to the right. E, F, Arrow in the low-magnification image indicates the specific WT1 cell of interest in the panel to the right. In magnified images, arrows indicate presumptive WT1+ axon terminals. Double labeling of these processes is confirmed in orthogonal views to the right of E, F. Scale bars: Low-magnification images, 100 μm; High-magnification images: A, C, D, 20 μm; B, E, F, 5 μm.
    Figure Legend Snippet: WT1-expressing neurons terminate in close proximity to populations of commissurally projecting interneurons. A, A 20-μm-thick section of a spinal cord 46 h after PRV-152 injection into the GS muscle on the left side and stained with antibodies to GFP (green) and WT1 (blue). At this time point, no WT1-expressing neurons on either side of the spinal cord have taken up the tracer. Contralateral region containing WT1-expressing cells (dashed box) is expanded to the right. B, To identify presumptive synapses on genetically defined interneuronal subtypes, we inspected the synaptotagmin+ “halo” (indicated by white arrows) surrounding each labeled nuclei for WT1+/synaptotagmin+ terminals. C–F, The 20-μm-thick sections cut from a P0 WT1CreERROSA26tdTomato spinal cord and stained with antibodies to tdTomato (red), the synaptic marker synatotagmin (blue), as well as (green) a nuclear marker of V2a cells (Chx10-, C), V1 cells (En1-, D), V0V cells (Evx1-, E), or DMRT3-expressing dI6 cells (F). WT1-expressing terminals (tdTomato+/synaptotagmin+ processes) were rare or absent nearby Chx10- or En1-expressing cells but were commonly seen in close proximity to Evx1+ and DMRT3+ neurons. C, D, Dashed boxes are expanded to the right. E, F, Arrow in the low-magnification image indicates the specific WT1 cell of interest in the panel to the right. In magnified images, arrows indicate presumptive WT1+ axon terminals. Double labeling of these processes is confirmed in orthogonal views to the right of E, F. Scale bars: Low-magnification images, 100 μm; High-magnification images: A, C, D, 20 μm; B, E, F, 5 μm.

    Techniques Used: Expressing, Injection, Staining, Labeling, Marker