anti bk α subunit antibody  (Alomone Labs)


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    Alomone Labs anti bk α subunit antibody
    Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK <t>α</t> subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P
    Anti Bk α Subunit Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bk α subunit antibody/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bk α subunit antibody - by Bioz Stars, 2022-07
    92/100 stars

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    1) Product Images from "BK channel inactivation gates daytime excitability in the circadian clock"

    Article Title: BK channel inactivation gates daytime excitability in the circadian clock

    Journal: Nature Communications

    doi: 10.1038/ncomms10837

    Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK α subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P
    Figure Legend Snippet: Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK α subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P

    Techniques Used: Expressing, Immunoprecipitation, Western Blot, Positive Control, Negative Control

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    Alomone Labs anti bk α subunit antibody
    Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK <t>α</t> subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P
    Anti Bk α Subunit Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bk α subunit antibody/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bk α subunit antibody - by Bioz Stars, 2022-07
    92/100 stars
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    Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK α subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P

    Journal: Nature Communications

    Article Title: BK channel inactivation gates daytime excitability in the circadian clock

    doi: 10.1038/ncomms10837

    Figure Lengend Snippet: Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK α subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P

    Article Snippet: Supernatant was collected and mixed with 1 μg of anti-BK α subunit antibody (anti-KCa1.1, #1184–1200, Alamone Labs; Jerusalem, Israel) and rotated for 2 h at 4 °C.

    Techniques: Expressing, Immunoprecipitation, Western Blot, Positive Control, Negative Control