rabbit polyclonal anti aqp4 antibody  (Alomone Labs)


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    • 86
    Name:
    Anti Aquaporin 4 AQP4 300 314 ATTO Fluor 594 Antibody
    Description:
    Anti Aquaporin 4 AQP4 300 314 Antibody AQP 014 is a highly specific antibody directed against an epitope of rat AQP4 The antibody can be used in western blot immunohistochemistry and immunocytochemistry applications It has been designed to recognize the Aquaporin 4 channel from rat mouse and human samples nAnti Aquaporin 4 AQP4 300 314 ATTO Fluor 594 Antibody AQP 014 AR is directly labeled with an ATTO 594 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa 594 Anti Aquaporin 4 AQP4 300 314 ATTO Fluor 594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers
    Catalog Number:
    AQP-014-AR
    Price:
    686.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal ATTO 594 (Red) Conjugated Primary Antibody
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    		Buy from Supplier

    Structured Review

    Alomone Labs rabbit polyclonal anti aqp4 antibody
    Anti Aquaporin 4 AQP4 300 314 ATTO Fluor 594 Antibody
    Anti Aquaporin 4 AQP4 300 314 Antibody AQP 014 is a highly specific antibody directed against an epitope of rat AQP4 The antibody can be used in western blot immunohistochemistry and immunocytochemistry applications It has been designed to recognize the Aquaporin 4 channel from rat mouse and human samples nAnti Aquaporin 4 AQP4 300 314 ATTO Fluor 594 Antibody AQP 014 AR is directly labeled with an ATTO 594 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa 594 Anti Aquaporin 4 AQP4 300 314 ATTO Fluor 594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers
    https://www.bioz.com/result/rabbit polyclonal anti aqp4 antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti aqp4 antibody - by Bioz Stars, 2021-09
    86/100 stars

    Images

    1) Product Images from "Vasopressin receptors V1a and V2 are not osmosensors"

    Article Title: Vasopressin receptors V1a and V2 are not osmosensors

    Journal: Physiological Reports

    doi: 10.14814/phy2.12519

    V1 a R-dependent downregulation of AQP4. (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P
    Figure Legend Snippet: V1 a R-dependent downregulation of AQP4. (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Techniques Used: Expressing, Permeability

    V1 a R-dependent internalization of AQP4. (A) Confocal laser scanning microscopy of oocytes expressing either AQP4 (left panel) or AQP4/V1 a R (right panel) immune-labeled for AQP4. The upper panels are representative images of oocytes exposed to control solution without vasopressin for 80 min. The middle panels are representative images of oocytes kept in control solution for 20 min and then treated with 1 μ mol/L vasopressin for 60 min. The lower panels are representative images of oocytes treated with a 50 mOsm hyperosmolar gradient for 30 sec every 10 min of an 80-min incubation period. (B) Oocyte plasma membrane fluorescence intensity normalized to that of the oocytes kept in control solution, n = 5 experiments with 3–6 oocytes per condition. The indicated groups were compared using one-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P
    Figure Legend Snippet: V1 a R-dependent internalization of AQP4. (A) Confocal laser scanning microscopy of oocytes expressing either AQP4 (left panel) or AQP4/V1 a R (right panel) immune-labeled for AQP4. The upper panels are representative images of oocytes exposed to control solution without vasopressin for 80 min. The middle panels are representative images of oocytes kept in control solution for 20 min and then treated with 1 μ mol/L vasopressin for 60 min. The lower panels are representative images of oocytes treated with a 50 mOsm hyperosmolar gradient for 30 sec every 10 min of an 80-min incubation period. (B) Oocyte plasma membrane fluorescence intensity normalized to that of the oocytes kept in control solution, n = 5 experiments with 3–6 oocytes per condition. The indicated groups were compared using one-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Techniques Used: Confocal Laser Scanning Microscopy, Expressing, Labeling, Incubation, Fluorescence

    Related Articles

    Immunostaining:

    Article Title: Vasopressin receptors V1a and V2 are not osmosensors
    Article Snippet: .. Sections of 2 μm were cut on a Leica RM 2126 microtome and immunostaining performed as described previously (Fenton et al. ) using a rabbit polyclonal anti-AQP4 antibody 1:5000 (Alamone Laboratories, Jerusalem, Israel). ..

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    • rabbit polyclonal anti aqp4 antibody  (Alomone Labs)
    86
    Alomone Labs rabbit polyclonal anti aqp4 antibody
    V1 a R-dependent downregulation of <t>AQP4.</t> (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P
    Rabbit Polyclonal Anti Aqp4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti aqp4 antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti aqp4 antibody - by Bioz Stars, 2021-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    V1 a R-dependent downregulation of AQP4. (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Journal: Physiological Reports

    Article Title: Vasopressin receptors V1a and V2 are not osmosensors

    doi: 10.14814/phy2.12519

    Figure Lengend Snippet: V1 a R-dependent downregulation of AQP4. (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Article Snippet: Sections of 2 μm were cut on a Leica RM 2126 microtome and immunostaining performed as described previously (Fenton et al. ) using a rabbit polyclonal anti-AQP4 antibody 1:5000 (Alamone Laboratories, Jerusalem, Israel).

    Techniques: Expressing, Permeability

    V1 a R-dependent internalization of AQP4. (A) Confocal laser scanning microscopy of oocytes expressing either AQP4 (left panel) or AQP4/V1 a R (right panel) immune-labeled for AQP4. The upper panels are representative images of oocytes exposed to control solution without vasopressin for 80 min. The middle panels are representative images of oocytes kept in control solution for 20 min and then treated with 1 μ mol/L vasopressin for 60 min. The lower panels are representative images of oocytes treated with a 50 mOsm hyperosmolar gradient for 30 sec every 10 min of an 80-min incubation period. (B) Oocyte plasma membrane fluorescence intensity normalized to that of the oocytes kept in control solution, n = 5 experiments with 3–6 oocytes per condition. The indicated groups were compared using one-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Journal: Physiological Reports

    Article Title: Vasopressin receptors V1a and V2 are not osmosensors

    doi: 10.14814/phy2.12519

    Figure Lengend Snippet: V1 a R-dependent internalization of AQP4. (A) Confocal laser scanning microscopy of oocytes expressing either AQP4 (left panel) or AQP4/V1 a R (right panel) immune-labeled for AQP4. The upper panels are representative images of oocytes exposed to control solution without vasopressin for 80 min. The middle panels are representative images of oocytes kept in control solution for 20 min and then treated with 1 μ mol/L vasopressin for 60 min. The lower panels are representative images of oocytes treated with a 50 mOsm hyperosmolar gradient for 30 sec every 10 min of an 80-min incubation period. (B) Oocyte plasma membrane fluorescence intensity normalized to that of the oocytes kept in control solution, n = 5 experiments with 3–6 oocytes per condition. The indicated groups were compared using one-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Article Snippet: Sections of 2 μm were cut on a Leica RM 2126 microtome and immunostaining performed as described previously (Fenton et al. ) using a rabbit polyclonal anti-AQP4 antibody 1:5000 (Alamone Laboratories, Jerusalem, Israel).

    Techniques: Confocal Laser Scanning Microscopy, Expressing, Labeling, Incubation, Fluorescence