rabbit polyclonal anti aqp4 antibody  (Alomone Labs)


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    Alomone Labs rabbit polyclonal anti aqp4 antibody
    V1 a R-dependent downregulation of <t>AQP4.</t> (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P
    Rabbit Polyclonal Anti Aqp4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti aqp4 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti aqp4 antibody - by Bioz Stars, 2022-01
    94/100 stars

    Images

    1) Product Images from "Vasopressin receptors V1a and V2 are not osmosensors"

    Article Title: Vasopressin receptors V1a and V2 are not osmosensors

    Journal: Physiological Reports

    doi: 10.14814/phy2.12519

    V1 a R-dependent downregulation of AQP4. (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P
    Figure Legend Snippet: V1 a R-dependent downregulation of AQP4. (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Techniques Used: Expressing, Permeability

    V1 a R-dependent internalization of AQP4. (A) Confocal laser scanning microscopy of oocytes expressing either AQP4 (left panel) or AQP4/V1 a R (right panel) immune-labeled for AQP4. The upper panels are representative images of oocytes exposed to control solution without vasopressin for 80 min. The middle panels are representative images of oocytes kept in control solution for 20 min and then treated with 1 μ mol/L vasopressin for 60 min. The lower panels are representative images of oocytes treated with a 50 mOsm hyperosmolar gradient for 30 sec every 10 min of an 80-min incubation period. (B) Oocyte plasma membrane fluorescence intensity normalized to that of the oocytes kept in control solution, n = 5 experiments with 3–6 oocytes per condition. The indicated groups were compared using one-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P
    Figure Legend Snippet: V1 a R-dependent internalization of AQP4. (A) Confocal laser scanning microscopy of oocytes expressing either AQP4 (left panel) or AQP4/V1 a R (right panel) immune-labeled for AQP4. The upper panels are representative images of oocytes exposed to control solution without vasopressin for 80 min. The middle panels are representative images of oocytes kept in control solution for 20 min and then treated with 1 μ mol/L vasopressin for 60 min. The lower panels are representative images of oocytes treated with a 50 mOsm hyperosmolar gradient for 30 sec every 10 min of an 80-min incubation period. (B) Oocyte plasma membrane fluorescence intensity normalized to that of the oocytes kept in control solution, n = 5 experiments with 3–6 oocytes per condition. The indicated groups were compared using one-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Techniques Used: Confocal Laser Scanning Microscopy, Expressing, Labeling, Incubation, Fluorescence

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    Alomone Labs rabbit polyclonal anti aqp4 antibody
    V1 a R-dependent downregulation of <t>AQP4.</t> (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P
    Rabbit Polyclonal Anti Aqp4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti aqp4 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti aqp4 antibody - by Bioz Stars, 2022-01
    94/100 stars
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    V1 a R-dependent downregulation of AQP4. (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Journal: Physiological Reports

    Article Title: Vasopressin receptors V1a and V2 are not osmosensors

    doi: 10.14814/phy2.12519

    Figure Lengend Snippet: V1 a R-dependent downregulation of AQP4. (A) Volume traces obtained from an uninjected oocyte (left panel) and an AQP4/V1 a R-expressing oocyte challenged with an osmotic gradient of 50 mOsm mannitol for 30 sec. (B) Relative water permeability of oocytes expressing AQP4 (open circles; n = 5) or coexpressing AQP4/V1 a R (filled circles, n = 20) exposed to 1 μ mol/L vasopressin as marked by the black bar. (C) Relative water permeability of oocytes expressing AQP4 (open circles; n = 6) or coexpressing AQP4/V1 a R (filled circles; n = 22) when exposed to repeated osmotic challenges. (D) Relative water permeability of oocytes coexpressing AQP4/mGluR1a and exposed to 500 μ mol/L glutamate as indicated by the black bar (filled symbols, n = 8) or kept in control solution (open symbols, n = 8, not exposed to glutamate). The groups were compared with two-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Article Snippet: Sections of 2 μm were cut on a Leica RM 2126 microtome and immunostaining performed as described previously (Fenton et al. ) using a rabbit polyclonal anti-AQP4 antibody 1:5000 (Alamone Laboratories, Jerusalem, Israel).

    Techniques: Expressing, Permeability

    V1 a R-dependent internalization of AQP4. (A) Confocal laser scanning microscopy of oocytes expressing either AQP4 (left panel) or AQP4/V1 a R (right panel) immune-labeled for AQP4. The upper panels are representative images of oocytes exposed to control solution without vasopressin for 80 min. The middle panels are representative images of oocytes kept in control solution for 20 min and then treated with 1 μ mol/L vasopressin for 60 min. The lower panels are representative images of oocytes treated with a 50 mOsm hyperosmolar gradient for 30 sec every 10 min of an 80-min incubation period. (B) Oocyte plasma membrane fluorescence intensity normalized to that of the oocytes kept in control solution, n = 5 experiments with 3–6 oocytes per condition. The indicated groups were compared using one-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Journal: Physiological Reports

    Article Title: Vasopressin receptors V1a and V2 are not osmosensors

    doi: 10.14814/phy2.12519

    Figure Lengend Snippet: V1 a R-dependent internalization of AQP4. (A) Confocal laser scanning microscopy of oocytes expressing either AQP4 (left panel) or AQP4/V1 a R (right panel) immune-labeled for AQP4. The upper panels are representative images of oocytes exposed to control solution without vasopressin for 80 min. The middle panels are representative images of oocytes kept in control solution for 20 min and then treated with 1 μ mol/L vasopressin for 60 min. The lower panels are representative images of oocytes treated with a 50 mOsm hyperosmolar gradient for 30 sec every 10 min of an 80-min incubation period. (B) Oocyte plasma membrane fluorescence intensity normalized to that of the oocytes kept in control solution, n = 5 experiments with 3–6 oocytes per condition. The indicated groups were compared using one-way analysis of variance (ANOVA) with Šídák’s multiple comparison post hoc test. * P

    Article Snippet: Sections of 2 μm were cut on a Leica RM 2126 microtome and immunostaining performed as described previously (Fenton et al. ) using a rabbit polyclonal anti-AQP4 antibody 1:5000 (Alamone Laboratories, Jerusalem, Israel).

    Techniques: Confocal Laser Scanning Microscopy, Expressing, Labeling, Incubation, Fluorescence