p2y2  (Alomone Labs)


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    Name:
    Anti P2Y2 Receptor Antibody
    Description:
    Anti P2Y2 Receptor Antibody APR 010 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot indirect flow cytometry immunoprecipitation immunocytochemistry and immunohistochemistry applications It has been designed to recognize P2RY2 in rat mouse and human samples
    Catalog Number:
    APR-010
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs p2y2
    Anti P2Y2 Receptor Antibody
    Anti P2Y2 Receptor Antibody APR 010 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot indirect flow cytometry immunoprecipitation immunocytochemistry and immunohistochemistry applications It has been designed to recognize P2RY2 in rat mouse and human samples
    https://www.bioz.com/result/p2y2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2y2 - by Bioz Stars, 2021-09
    93/100 stars

    Images

    1) Product Images from "Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries"

    Article Title: Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries

    Journal:

    doi: 10.1038/sj.bjp.0707120

    RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors
    Figure Legend Snippet: RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Sequencing

    RT-PCR and immunoblot analysis of P2Y receptor expression in pig coronary and ear arteries. Agarose gel electrophoresis ( a–c ) showing size marker (MW) and PCR products from coronary (PCA) and ear (PEA) arteries or no template (CON) for p2ry2 (
    Figure Legend Snippet: RT-PCR and immunoblot analysis of P2Y receptor expression in pig coronary and ear arteries. Agarose gel electrophoresis ( a–c ) showing size marker (MW) and PCR products from coronary (PCA) and ear (PEA) arteries or no template (CON) for p2ry2 (

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis, Marker, Polymerase Chain Reaction

    2) Product Images from "Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer"

    Article Title: Impact of CD39 and purinergic signalling on the growth and metastasis of colorectal cancer

    Journal: Purinergic Signalling

    doi: 10.1007/s11302-011-9228-9

    Purinergic studies in human CRC. Protein expression levels of CD39, P2X7 and P2Y2 ( a – c ) and localization of P2Y2 ( d , e ) was determined in human CRC. Changes in protein levels were observed for P2Y2 ( c ), which was significantly less in T3N + M0
    Figure Legend Snippet: Purinergic studies in human CRC. Protein expression levels of CD39, P2X7 and P2Y2 ( a – c ) and localization of P2Y2 ( d , e ) was determined in human CRC. Changes in protein levels were observed for P2Y2 ( c ), which was significantly less in T3N + M0

    Techniques Used: Expressing

    CD39 and P2 receptors in CRC. a In orthotopic tumors, CD39, P2X7 and P2Y2 mRNA appeared at comparable levels to those seen in control livers. b As expected, mouse CD39 mRNA was significantly lower expressed in genetically deficient over wild-type and
    Figure Legend Snippet: CD39 and P2 receptors in CRC. a In orthotopic tumors, CD39, P2X7 and P2Y2 mRNA appeared at comparable levels to those seen in control livers. b As expected, mouse CD39 mRNA was significantly lower expressed in genetically deficient over wild-type and

    Techniques Used:

    3) Product Images from "Nitric Oxide-mediated Modulation of Synaptic Activity by Astrocytic P2Y Receptors"

    Article Title: Nitric Oxide-mediated Modulation of Synaptic Activity by Astrocytic P2Y Receptors

    Journal: The Journal of General Physiology

    doi: 10.1085/jgp.200810043

    Representative traces of [Ca 2+ ] i response in solitary neurons and astrocytes in low density cultures stimulated with 30 μM UTP (A); 100 μM ATP (B). The traces shown are from a single neuron and astrocyte representing 15 neurons and 40 astrocytes from eight experiments. (C and D) Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and synaptophysin (red). P2Y2 and P2Y4 receptors are expressed only in astrocytes. P2Y2 and P2Y4 receptor immunofluorescence was not colocalized with synaptophysin fluorescence at synaptic terminals.
    Figure Legend Snippet: Representative traces of [Ca 2+ ] i response in solitary neurons and astrocytes in low density cultures stimulated with 30 μM UTP (A); 100 μM ATP (B). The traces shown are from a single neuron and astrocyte representing 15 neurons and 40 astrocytes from eight experiments. (C and D) Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and synaptophysin (red). P2Y2 and P2Y4 receptors are expressed only in astrocytes. P2Y2 and P2Y4 receptor immunofluorescence was not colocalized with synaptophysin fluorescence at synaptic terminals.

    Techniques Used: Immunofluorescence, Fluorescence

    Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and neuronal markers anti-MAP-2 (red). Both P2Y2 and P2Y4 receptor immunostaining is not seen in neuronal cell bodies or processes but astrocytes are profusely stained. In merged images, some pixels appear yellow because of the neurons (red) overlying the brightly stained (green) astrocytes.
    Figure Legend Snippet: Confocal images of mixed hippocampal cultures immunostained with anti-P2Y receptors (green) and neuronal markers anti-MAP-2 (red). Both P2Y2 and P2Y4 receptor immunostaining is not seen in neuronal cell bodies or processes but astrocytes are profusely stained. In merged images, some pixels appear yellow because of the neurons (red) overlying the brightly stained (green) astrocytes.

    Techniques Used: Immunostaining, Staining

    Related Articles

    Western Blot:

    Article Title: Role for ovarian hormones in purinoceptor-dependent natriuresis
    Article Snippet: .. Representative Western blots for inner medullary homogenates from male, ovary-intact female and OVX Sprague Dawley rats incubated with anti-P2Y2 receptor antibody (A) or anti-P2Y4 receptor antibody (APR-010, APR-006, respectively, Alomone Labs) in the presence (right) and absence (left) of the respective blocking peptide. ..

    Incubation:

    Article Title: Role for ovarian hormones in purinoceptor-dependent natriuresis
    Article Snippet: .. Representative Western blots for inner medullary homogenates from male, ovary-intact female and OVX Sprague Dawley rats incubated with anti-P2Y2 receptor antibody (A) or anti-P2Y4 receptor antibody (APR-010, APR-006, respectively, Alomone Labs) in the presence (right) and absence (left) of the respective blocking peptide. ..

    Article Title: Role for ovarian hormones in purinoceptor-dependent natriuresis
    Article Snippet: .. Inner medulla protein lysates were transferred and incubated with rabbit anti-P2Y2 or anti-P2Y4 receptor primary antibody (APR-010, APR-006, respectively, Alomone Labs) at 1:1500 dilution at 4 °C overnight. ..

    Article Title: Differential Effects of Purinergic Signaling in Gastric Cancer-Derived Cells Through P2Y and P2X Receptors
    Article Snippet: .. Cells were permeabilized with 0.2% Triton X-100 in PBS-CM for 10 min at room temperature (RT) and blocked with PBS–1% bis(trimethylsilyl)acetamide (BSA) for 30 min. Then cells were incubated with primary antibodies against P2X4R, P2X7R, or P2Y2 R (1:100) (Alomone Labs, Jerusalem, Israel) in a humidified chamber overnight at 4°C. ..

    Article Title: Changes in melatonin receptor expression in a murine model of glaucoma
    Article Snippet: .. Then, the following primary antibodies diluted in PBS containing 0.1% TX-100 were incubated at 4 °C overnight: goat anti-MT1 (Santa Cruz Biotechnologies, Inc. Santa Cruz, CA; sc-15204, 1:100), goat anti-MT2 (Alomone Labs, Jerusalem, Israel; APR-010, 1:100), and goat anti-GPR50 (Santa Cruz Biotechnologies; sc-15215, 1:75). ..

    Article Title: Differential Effects of Purinergic Signaling in Gastric Cancer-Derived Cells Through P2Y and P2X Receptors
    Article Snippet: .. Membranes were blocked with buffer [5% nonfat dry milk in Tris-buffered saline (TBS) with 0.05% Tween-20 (TBS-T)] at room temperature for 1 h. Blots were incubated with primary antibodies against P2X4R, P2X7R, P2Y2 R, P2Y1 R (Alomone Labs, Jerusalem, Israel), and β-actin (Sigma-Aldrich, St. Louis, MO, USA) at 4°C overnight. ..

    Blocking Assay:

    Article Title: Role for ovarian hormones in purinoceptor-dependent natriuresis
    Article Snippet: .. Representative Western blots for inner medullary homogenates from male, ovary-intact female and OVX Sprague Dawley rats incubated with anti-P2Y2 receptor antibody (A) or anti-P2Y4 receptor antibody (APR-010, APR-006, respectively, Alomone Labs) in the presence (right) and absence (left) of the respective blocking peptide. ..

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  • About
  • News
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  • Team
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  • 93
    Alomone Labs p2y2
    RT-PCR and sequence analysis of porcine <t>P2Y2</t> , P2Y4 and P2Y6 receptors
    P2y2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2y2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2y2 - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors

    Journal:

    Article Title: Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries

    doi: 10.1038/sj.bjp.0707120

    Figure Lengend Snippet: RT-PCR and sequence analysis of porcine P2Y2 , P2Y4 and P2Y6 receptors

    Article Snippet: Antibodies directed against sequence-specific peptides from P2Y2 , P2Y4 and P2Y6 receptors were from Alomone Labs/Caltag-Medsystems (Buckingham, UK), while HRP-conjugated goat anti-rabbit antibody was from DAKO/Cytomation (Ely, UK).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing

    RT-PCR and immunoblot analysis of P2Y receptor expression in pig coronary and ear arteries. Agarose gel electrophoresis ( a–c ) showing size marker (MW) and PCR products from coronary (PCA) and ear (PEA) arteries or no template (CON) for p2ry2 (

    Journal:

    Article Title: Evidence for the Expression of Multiple Uracil Nucleotide-Stimulated P2 Receptors Coupled to Smooth Muscle Contraction in Porcine Isolated Arteries

    doi: 10.1038/sj.bjp.0707120

    Figure Lengend Snippet: RT-PCR and immunoblot analysis of P2Y receptor expression in pig coronary and ear arteries. Agarose gel electrophoresis ( a–c ) showing size marker (MW) and PCR products from coronary (PCA) and ear (PEA) arteries or no template (CON) for p2ry2 (

    Article Snippet: Antibodies directed against sequence-specific peptides from P2Y2 , P2Y4 and P2Y6 receptors were from Alomone Labs/Caltag-Medsystems (Buckingham, UK), while HRP-conjugated goat anti-rabbit antibody was from DAKO/Cytomation (Ely, UK).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis, Marker, Polymerase Chain Reaction